AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83416

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83416.t1 MTR_8g071280 97.483 596 15 0 1 596 1 596 0.0 1211
MS.gene83416.t1 MTR_5g026930 76.333 600 136 3 1 596 1 598 0.0 961
MS.gene83416.t1 MTR_5g026930 76.447 501 112 3 1 497 1 499 0.0 801
MS.gene83416.t1 MTR_1g101370 49.262 542 262 9 57 588 79 617 0.0 550
MS.gene83416.t1 MTR_8g432680 49.451 546 258 10 52 588 94 630 0.0 541
MS.gene83416.t1 MTR_0019s0120 48.921 556 266 10 43 590 83 628 0.0 533
MS.gene83416.t1 MTR_7g076170 50.947 528 245 10 71 588 102 625 0.0 529
MS.gene83416.t1 MTR_8g100075 45.636 550 283 10 58 595 82 627 9.61e-175 509
MS.gene83416.t1 MTR_8g055840 42.314 605 324 10 10 595 9 607 2.41e-168 493
MS.gene83416.t1 MTR_5g040360 45.109 552 290 8 45 590 57 601 5.07e-167 489
MS.gene83416.t1 MTR_7g006060 45.554 551 282 10 45 582 20 565 6.66e-167 488
MS.gene83416.t1 MTR_4g098890 47.637 529 262 8 66 587 82 602 4.03e-166 514
MS.gene83416.t1 MTR_3g040370 43.023 602 308 12 16 595 16 604 3.33e-165 484
MS.gene83416.t1 MTR_8g432620 41.423 548 293 8 52 588 70 600 2.49e-156 461
MS.gene83416.t1 MTR_7g076150 41.219 558 297 9 45 588 47 587 3.31e-155 458
MS.gene83416.t1 MTR_5g080720 42.831 544 286 11 53 585 108 637 1.37e-152 453
MS.gene83416.t1 MTR_0019s0120 49.351 462 217 9 43 497 83 534 4.44e-151 446
MS.gene83416.t1 MTR_3g068990 42.251 542 286 11 57 587 142 667 8.59e-146 437
MS.gene83416.t1 MTR_1g442840 37.460 622 333 10 11 591 12 618 5.80e-145 433
MS.gene83416.t1 MTR_1g075900 38.782 624 324 11 11 592 11 618 1.39e-144 432
MS.gene83416.t1 MTR_1g029730 42.748 524 269 13 79 587 151 658 9.13e-144 431
MS.gene83416.t1 MTR_1g069785 38.833 600 326 12 11 591 11 588 5.27e-143 427
MS.gene83416.t1 MTR_1g442840 41.833 502 271 6 101 591 14 505 5.34e-143 424
MS.gene83416.t1 MTR_3g096480 42.424 528 267 12 79 588 268 776 2.96e-137 419
MS.gene83416.t1 MTR_5g021100 41.333 525 273 13 79 588 329 833 3.13e-137 420
MS.gene83416.t1 MTR_8g076650 40.146 548 301 11 52 588 359 890 2.90e-136 419
MS.gene83416.t1 MTR_8g093880 40.000 565 299 14 44 588 262 806 1.63e-133 410
MS.gene83416.t1 MTR_5g080720 43.035 481 250 10 53 523 108 574 1.80e-132 399
MS.gene83416.t1 MTR_7g076150 43.384 461 233 8 139 588 1 444 4.99e-132 394
MS.gene83416.t1 MTR_5g083150 38.846 520 286 9 79 588 101 598 1.19e-129 393
MS.gene83416.t1 MTR_3g088790 40.000 525 285 11 79 588 233 742 2.83e-126 389
MS.gene83416.t1 MTR_3g071530 39.808 520 277 11 79 588 153 646 5.96e-126 385
MS.gene83416.t1 MTR_1g051055 36.975 595 333 12 22 588 23 603 4.66e-124 379
MS.gene83416.t1 MTR_1g022255 36.973 522 288 13 78 588 208 699 5.00e-107 337
MS.gene83416.t1 MTR_4g083030 33.213 554 316 13 59 585 75 601 6.28e-94 300
MS.gene83416.t1 MTR_3g074780 30.755 556 337 15 64 588 150 688 3.50e-92 298
MS.gene83416.t1 MTR_5g064080 31.492 543 318 15 73 585 149 667 6.97e-92 297
MS.gene83416.t1 MTR_1g022255 33.504 391 223 11 78 458 208 571 2.20e-60 211
MS.gene83416.t1 MTR_5g033760 45.455 66 36 0 531 596 41 106 2.90e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83416.t1 AT4G19120 73.277 595 148 3 7 596 10 598 0.0 942
MS.gene83416.t1 AT4G19120 73.277 595 148 3 7 596 10 598 0.0 942
MS.gene83416.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene83416.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene83416.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene83416.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene83416.t1 AT4G19120 73.790 496 119 3 7 497 10 499 0.0 785
MS.gene83416.t1 AT4G10440 47.004 534 270 9 70 594 93 622 7.50e-180 523
MS.gene83416.t1 AT4G00750 44.079 608 307 11 18 595 25 629 1.02e-179 523
MS.gene83416.t1 AT1G33170 47.259 529 265 10 70 588 110 634 1.96e-176 514
MS.gene83416.t1 AT2G45750 47.290 535 266 9 67 588 84 615 5.74e-176 513
MS.gene83416.t1 AT4G18030 42.414 613 307 10 10 592 11 607 6.18e-171 499
MS.gene83416.t1 AT1G26850 45.321 545 282 9 61 595 77 615 1.11e-165 486
MS.gene83416.t1 AT1G26850 45.321 545 282 9 61 595 77 615 1.11e-165 486
MS.gene83416.t1 AT4G00740 44.275 524 270 7 73 588 84 593 1.06e-162 478
MS.gene83416.t1 AT4G00740 44.275 524 270 7 73 588 84 593 1.06e-162 478
MS.gene83416.t1 AT2G43200 45.908 501 262 8 70 565 91 587 2.60e-155 459
MS.gene83416.t1 AT2G43200 45.908 501 262 8 70 565 91 587 2.60e-155 459
MS.gene83416.t1 AT2G39750 43.831 543 280 11 56 587 163 691 1.23e-152 455
MS.gene83416.t1 AT1G77260 40.455 571 309 14 28 587 105 655 2.22e-144 433
MS.gene83416.t1 AT3G23300 38.972 603 331 8 16 591 12 604 4.99e-143 428
MS.gene83416.t1 AT3G23300 38.972 603 331 8 16 591 12 604 4.99e-143 428
MS.gene83416.t1 AT1G04430 37.778 630 338 11 1 591 2 616 6.94e-142 425
MS.gene83416.t1 AT1G04430 37.778 630 338 11 1 591 2 616 6.94e-142 425
MS.gene83416.t1 AT1G04430 37.778 630 338 11 1 591 2 616 6.94e-142 425
MS.gene83416.t1 AT5G06050 40.556 540 292 11 61 587 140 663 1.70e-141 426
MS.gene83416.t1 AT4G14360 41.155 537 292 7 69 591 75 601 8.98e-140 419
MS.gene83416.t1 AT4G14360 41.155 537 292 7 69 591 75 601 8.98e-140 419
MS.gene83416.t1 AT2G40280 39.763 591 306 14 21 596 34 589 1.60e-138 416
MS.gene83416.t1 AT3G56080 43.750 496 251 14 79 564 114 591 8.07e-137 412
MS.gene83416.t1 AT2G34300 41.561 551 284 14 56 588 227 757 1.38e-136 416
MS.gene83416.t1 AT2G34300 41.561 551 284 14 56 588 227 757 1.38e-136 416
MS.gene83416.t1 AT2G34300 41.561 551 284 14 56 588 227 757 1.38e-136 416
MS.gene83416.t1 AT1G29470 40.653 551 290 13 56 588 226 757 9.89e-135 412
MS.gene83416.t1 AT1G29470 40.653 551 290 13 56 588 226 757 9.89e-135 412
MS.gene83416.t1 AT5G64030 41.321 530 276 13 79 591 308 819 5.24e-133 409
MS.gene83416.t1 AT5G14430 38.833 600 325 11 17 588 19 604 4.55e-132 400
MS.gene83416.t1 AT5G14430 38.667 600 326 11 17 588 19 604 1.12e-130 396
MS.gene83416.t1 AT3G51070 40.684 526 279 11 79 588 381 889 4.55e-129 400
MS.gene83416.t1 AT3G10200 35.655 603 351 10 6 588 3 588 4.18e-128 389
MS.gene83416.t1 AT1G26850 44.601 426 222 7 61 478 77 496 5.25e-125 378
MS.gene83416.t1 AT3G56080 43.956 455 227 14 79 523 114 550 6.92e-123 374
MS.gene83416.t1 AT5G04060 35.893 599 339 13 17 588 17 597 1.93e-121 372
MS.gene83416.t1 AT3G10200 36.413 552 316 9 6 539 3 537 1.62e-116 359
MS.gene83416.t1 AT3G51070 42.325 456 233 10 79 518 381 822 8.54e-116 364
MS.gene83416.t1 AT1G19430 37.143 525 284 15 78 588 230 722 4.60e-108 341
MS.gene83416.t1 AT1G19430 37.143 525 284 15 78 588 230 722 4.60e-108 341
MS.gene83416.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene83416.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene83416.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene83416.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene83416.t1 AT1G78240 30.642 545 329 13 73 585 149 676 2.24e-88 288
MS.gene83416.t1 AT1G78240 30.642 545 329 13 73 585 149 676 2.24e-88 288
MS.gene83416.t1 AT2G03480 30.851 564 331 13 55 585 64 601 2.35e-86 281
MS.gene83416.t1 AT2G03480 31.150 565 317 14 55 585 64 590 5.79e-86 279
MS.gene83416.t1 AT1G13860 33.477 463 259 14 154 587 2 444 1.62e-78 255
MS.gene83416.t1 AT1G19430 34.684 395 216 13 78 459 230 595 1.08e-63 220
MS.gene83416.t1 AT2G03480 28.152 341 222 6 55 380 64 396 1.80e-42 158

Find 155 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGTTCCATCTTTCATTTC+TGG 0.146770 8.4:-32328733 None:intergenic
TTCTTCGTCCTGGAGGATTT+TGG 0.151747 8.4:+32329536 MS.gene83416:CDS
TTCTATATGGGTGTAATCTT+TGG 0.198314 8.4:+32327838 MS.gene83416:CDS
CTGCTCTTTGGCCATCTAAT+TGG 0.211320 8.4:-32328446 None:intergenic
ATGTGGACTTTCTTTCTATA+TGG 0.220950 8.4:+32327825 MS.gene83416:CDS
CCCATCAAATTCTTTCGATA+TGG 0.245961 8.4:+32329311 MS.gene83416:CDS
TAGCTGGGGTGGTGATTTAC+TGG 0.259336 8.4:+32329167 MS.gene83416:CDS
CGGTAAATATGTTGATTTAA+TGG 0.261309 8.4:+32328699 MS.gene83416:CDS
CAATCAACATTGGTTGAAAA+AGG 0.293240 8.4:+32328624 MS.gene83416:CDS
TCAAACGTGACGGCAGTAAT+TGG 0.299230 8.4:+32329947 MS.gene83416:CDS
ACTTATTGATGATGATTTCT+TGG 0.300333 8.4:-32327914 None:intergenic
ATGGATATGAATACGGAATA+TGG 0.323237 8.4:+32330036 MS.gene83416:CDS
CGAATTCTGTCCATGGAATA+AGG 0.325163 8.4:-32329350 None:intergenic
GCTAGGCTGTCATCGCATTT+AGG 0.327506 8.4:-32329766 None:intergenic
ACATATTTACCGACACCGTT+CGG 0.328019 8.4:-32328688 None:intergenic
TGTGGACTTTCTTTCTATAT+GGG 0.328602 8.4:+32327826 MS.gene83416:CDS
ATTCGAACGGCCATTGATAC+TGG 0.329336 8.4:+32328754 MS.gene83416:CDS
CAACATTCATCTCTGCTCTT+TGG 0.332417 8.4:-32328458 None:intergenic
GGAGAGAAATTCCTCTTTCC+TGG 0.334164 8.4:+32328649 MS.gene83416:CDS
CTTATTGATGATGATTTCTT+GGG 0.337942 8.4:-32327913 None:intergenic
CGTGGTATCCCTGCAATTCT+TGG 0.339517 8.4:+32329261 MS.gene83416:CDS
CATATTTACCGACACCGTTC+GGG 0.351894 8.4:-32328687 None:intergenic
TTACAGAGATGGAAGAAGTA+TGG 0.359526 8.4:+32328332 MS.gene83416:intron
TAGTTCCATCTTTCATTTCT+GGG 0.364638 8.4:-32328732 None:intergenic
TATGACCTCCTTCATCTTGC+AGG 0.378268 8.4:+32330309 MS.gene83416:CDS
TGTTTGTTAATCCAGTCATA+TGG 0.383257 8.4:-32328595 None:intergenic
TTATTGTAACAATCATTAAC+AGG 0.384939 8.4:-32329721 None:intergenic
ACGACAAAACAAGTGCGAAT+TGG 0.387458 8.4:-32329811 None:intergenic
AGCTCAGGTTCAATTCGCTT+TGG 0.387480 8.4:+32329236 MS.gene83416:CDS
CTTATTCCATGGACAGAATT+CGG 0.393587 8.4:+32329351 MS.gene83416:CDS
AGCCACCAGGTCGTAAAATT+CGG 0.398973 8.4:-32330687 None:intergenic
GATCCAAAGTTTAAGAAAAT+AGG 0.401896 8.4:+32329838 MS.gene83416:CDS
GCAAGGACACCAAGAATTGC+AGG 0.407630 8.4:-32329270 None:intergenic
TGAGCTTGGTGGTTATCTCT+TGG 0.408024 8.4:-32329219 None:intergenic
TGAAATGAAGTACGTATTGT+TGG 0.409457 8.4:+32330657 MS.gene83416:CDS
CCTTGATTGATGATCCTGTA+TGG 0.411232 8.4:+32330073 MS.gene83416:CDS
ATCTTATCAGTCCCAAGCTC+AGG 0.412393 8.4:-32330003 None:intergenic
GAAGTACGTATTGTTGGAAA+TGG 0.413240 8.4:+32330663 MS.gene83416:CDS
TGATAGTCAATACTGCATTC+AGG 0.414829 8.4:-32327964 None:intergenic
TGATACAATTCTATCGTTCT+CGG 0.415528 8.4:-32327863 None:intergenic
CCCTGTGGCAATCATATTTG+TGG 0.438188 8.4:+32327798 MS.gene83416:CDS
ATCCTATCGGTTCATAATGT+TGG 0.442728 8.4:+32328355 MS.gene83416:CDS
ACGGCCATTGATACTGGTTG+TGG 0.443198 8.4:+32328760 MS.gene83416:CDS
CTCCATGTCACCTCCCTGCT+CGG 0.455405 8.4:-32330797 None:intergenic
ACTGGATCGTGGAATTCTTA+CGG 0.459338 8.4:+32329185 MS.gene83416:CDS
TCAGAAATGTTCCATATGAC+TGG 0.460287 8.4:+32328584 MS.gene83416:intron
ACCCTTAGCAATAGTTGTAA+TGG 0.466423 8.4:-32330746 None:intergenic
TGTCCATGGAATAAGGCATC+TGG 0.467386 8.4:-32329343 None:intergenic
GGTACCAGCTTCCAATTAGA+TGG 0.467802 8.4:+32328435 MS.gene83416:CDS
GGAGTATACCATGCTGAATC+TGG 0.477004 8.4:-32329790 None:intergenic
GTTTATGATCGAGGCCTCAT+TGG 0.482541 8.4:+32330141 MS.gene83416:CDS
AAGAAGTTGATCCCTGAGCT+TGG 0.483280 8.4:+32329991 MS.gene83416:CDS
TCGAAAGAATTTGATGGGAA+TGG 0.484261 8.4:-32329306 None:intergenic
TGCCATTACAACTATTGCTA+AGG 0.485514 8.4:+32330744 MS.gene83416:CDS
AAATGTGATGGATATGAATA+CGG 0.485516 8.4:+32330029 MS.gene83416:CDS
ATCCTCCAGGACGAAGAATC+CGG 0.487693 8.4:-32329531 None:intergenic
GCCATATCGAAAGAATTTGA+TGG 0.494406 8.4:-32329312 None:intergenic
CGAACCATTGAGATTCGATC+TGG 0.496305 8.4:-32329904 None:intergenic
GAAAAGTGGTAGACAAGAGT+AGG 0.497707 8.4:+32327770 MS.gene83416:CDS
GATCTGCGCTCGTAGTTGAT+TGG 0.499029 8.4:-32329574 None:intergenic
GAAGAATAATGAAGGAAAAG+TGG 0.501394 8.4:+32327756 MS.gene83416:CDS
AGAAGTTGATCCCTGAGCTT+GGG 0.508058 8.4:+32329992 MS.gene83416:CDS
AAGAGATAACCACCAAGCTC+AGG 0.511627 8.4:+32329221 MS.gene83416:CDS
AGCTGGTACCCATCCGGAGG+AGG 0.516830 8.4:-32328422 None:intergenic
TCATTGGAACGTTCCACGAC+TGG 0.519029 8.4:+32330157 MS.gene83416:CDS
TGAAGAGTCCTGCAAGATGA+AGG 0.520104 8.4:-32330317 None:intergenic
TTGGCCATCTAATTGGAAGC+TGG 0.520718 8.4:-32328439 None:intergenic
CTGATCCCAGAAATGAAAGA+TGG 0.520799 8.4:+32328727 MS.gene83416:CDS
GGTGTAACATGCAACCGCTC+GGG 0.520828 8.4:-32329883 None:intergenic
ATTGGAAGCTGGTACCCATC+CGG 0.522262 8.4:-32328428 None:intergenic
CGAATTGAACCTGAGCTTGG+TGG 0.522362 8.4:-32329230 None:intergenic
GCTCCAGATGCCTTATTCCA+TGG 0.526600 8.4:+32329340 MS.gene83416:CDS
TGCTTAGTTCCTCCTCCGGA+TGG 0.527581 8.4:+32328413 MS.gene83416:CDS
TGGTGTAACATGCAACCGCT+CGG 0.527597 8.4:-32329884 None:intergenic
AAATTCCTCTTTCCTGGTGG+AGG 0.529642 8.4:+32328655 MS.gene83416:CDS
AGAATGCTTAGTTCCTCCTC+CGG 0.532704 8.4:+32328409 MS.gene83416:CDS
ACCACATTCATAACCCATAC+AGG 0.533600 8.4:-32330087 None:intergenic
CTCGTAGTTGATTGGTGGAC+CGG 0.535746 8.4:-32329566 None:intergenic
TAGTACCTCCACCAGGAAAG+AGG 0.538108 8.4:-32328660 None:intergenic
CACTCCATGCACTGATCCAA+GGG 0.540931 8.4:+32327993 MS.gene83416:CDS
CACCCCACAACCAGTATCAA+TGG 0.542219 8.4:-32328764 None:intergenic
AAGCGAATTGAACCTGAGCT+TGG 0.543694 8.4:-32329233 None:intergenic
CGGCCATTGATACTGGTTGT+GGG 0.544765 8.4:+32328761 MS.gene83416:CDS
GCAGAGATGAATGTTGGTAC+AGG 0.545315 8.4:+32328465 MS.gene83416:CDS
TACCGAGCAGGGAGGTGACA+TGG 0.545935 8.4:+32330795 MS.gene83416:CDS
TGATAAGATAAGAAATGTGA+TGG 0.548109 8.4:+32330017 MS.gene83416:CDS
CGAATCTCAATGGTTCGTCA+TGG 0.550079 8.4:+32329910 MS.gene83416:CDS
GTTCAATTCGCTTTGGAGCG+TGG 0.551979 8.4:+32329243 MS.gene83416:CDS
ATCATATTTGTGGTGCTATG+TGG 0.553513 8.4:+32327808 MS.gene83416:CDS
CTGCGCTCGTAGTTGATTGG+TGG 0.554325 8.4:-32329571 None:intergenic
ACCACAAATATGATTGCCAC+AGG 0.556765 8.4:-32327799 None:intergenic
TTAATATTCTTGAAATACAC+CGG 0.559268 8.4:+32329512 MS.gene83416:CDS
ACACTCCATGCACTGATCCA+AGG 0.560389 8.4:+32327992 MS.gene83416:CDS
ATACACCGGATTCTTCGTCC+TGG 0.561320 8.4:+32329526 MS.gene83416:CDS
AACTACGAGCGCAGATCGCG+TGG 0.563131 8.4:+32329580 MS.gene83416:CDS
TACACCAGATCGAATCTCAA+TGG 0.564334 8.4:+32329900 MS.gene83416:CDS
CCATATCGAAAGAATTTGAT+GGG 0.566568 8.4:-32329311 None:intergenic
GTCTTACATCAATTTCTAAG+TGG 0.567739 8.4:+32329860 MS.gene83416:CDS
ACTATGTTCCCGAACGGTGT+CGG 0.567956 8.4:+32328679 MS.gene83416:CDS
TCTCGAAAATTGGAGGGCAA+TGG 0.568556 8.4:-32328382 None:intergenic
CTCCCTGTGGTTTATGATCG+AGG 0.568992 8.4:+32330132 MS.gene83416:CDS
ACTGCATTCAGGAAAACTGA+TGG 0.569719 8.4:-32327953 None:intergenic
GAATCTCAATGGTTCGTCAT+GGG 0.570523 8.4:+32329911 MS.gene83416:CDS
CGCACGAGAGCATAGCCACC+AGG 0.572650 8.4:-32330700 None:intergenic
TCGTACAGAAGATACCGAGC+AGG 0.572788 8.4:+32330783 MS.gene83416:CDS
AGGCCTCGATCATAAACCAC+AGG 0.572832 8.4:-32330135 None:intergenic
TCTTCACTGCAGAAAGCCAC+AGG 0.573575 8.4:+32330334 MS.gene83416:CDS
CCATACAGGATCATCAATCA+AGG 0.575427 8.4:-32330073 None:intergenic
TATTTCATAATTACCAGTCG+TGG 0.575538 8.4:-32330170 None:intergenic
GACAAGACCCAAAATCCTCC+AGG 0.575555 8.4:-32329544 None:intergenic
CGGGCGATGAAGAATAATGA+AGG 0.575646 8.4:+32327748 None:intergenic
GCTTAGTTCCTCCTCCGGAT+GGG 0.577164 8.4:+32328414 MS.gene83416:CDS
CAAAGAGCAGAGATGAATGT+TGG 0.578527 8.4:+32328459 MS.gene83416:CDS
ACGAGCGCAGATCGCGTGGA+TGG 0.579590 8.4:+32329584 MS.gene83416:CDS
AACAGGAGACTTCTGCCATA+CGG 0.581022 8.4:-32329704 None:intergenic
TTGGTGTCCTTGCTACACAC+AGG 0.584328 8.4:+32329280 MS.gene83416:CDS
CACCGGATTCTTCGTCCTGG+AGG 0.589366 8.4:+32329529 MS.gene83416:CDS
CTTGATTGATGATCCTGTAT+GGG 0.589605 8.4:+32330074 MS.gene83416:CDS
TTATTGATGATGATTTCTTG+GGG 0.593785 8.4:-32327912 None:intergenic
CTATTGCTAAGGGTATGCGA+TGG 0.603394 8.4:+32330755 MS.gene83416:CDS
GTGGTACCGAATTCTGTCCA+TGG 0.604736 8.4:-32329357 None:intergenic
GAGAAATTCCTCTTTCCTGG+TGG 0.605663 8.4:+32328652 MS.gene83416:CDS
AACAGAAGTCCAATCAACAT+TGG 0.605779 8.4:+32328614 MS.gene83416:CDS
TATTGCTAAGGGTATGCGAT+GGG 0.605922 8.4:+32330756 MS.gene83416:CDS
AGGCTGTCATCGCATTTAGG+TGG 0.605988 8.4:-32329763 None:intergenic
GCCATTACAACTATTGCTAA+GGG 0.606001 8.4:+32330745 MS.gene83416:CDS
TCTTATCAGTCCCAAGCTCA+GGG 0.607315 8.4:-32330002 None:intergenic
GTCATCGCATTTAGGTGGAT+AGG 0.608032 8.4:-32329758 None:intergenic
ATTACCCTTGGATCAGTGCA+TGG 0.610417 8.4:-32327997 None:intergenic
ATGGAAGCCTGTGTGTAGCA+AGG 0.611575 8.4:-32329287 None:intergenic
GGTGGTGATTTACTGGATCG+TGG 0.619774 8.4:+32329174 MS.gene83416:CDS
GGAAGAAGTATGGATCCTAT+CGG 0.630329 8.4:+32328342 MS.gene83416:CDS
TCCTGTATGGGTTATGAATG+TGG 0.630835 8.4:+32330086 MS.gene83416:CDS
TGCTGTGAATACACTCCCTG+TGG 0.631567 8.4:+32330119 MS.gene83416:CDS
ACCATGCTGAATCTGGTGCT+AGG 0.638733 8.4:-32329783 None:intergenic
GCCTAGCACCAGATTCAGCA+TGG 0.641516 8.4:+32329782 MS.gene83416:CDS
CAAGAGTAGGATTGTCCCTG+TGG 0.644091 8.4:+32327783 MS.gene83416:CDS
GAAAGATGGAACTATTCGAA+CGG 0.647942 8.4:+32328741 MS.gene83416:CDS
GGAGGTACTATGTTCCCGAA+CGG 0.648294 8.4:+32328673 MS.gene83416:CDS
CGTACAGAAGATACCGAGCA+GGG 0.654224 8.4:+32330784 MS.gene83416:CDS
TATTGATGATGATTTCTTGG+GGG 0.655952 8.4:-32327911 None:intergenic
GCTAGTACTTTCAAACGTGA+CGG 0.659772 8.4:+32329937 MS.gene83416:CDS
CAAGGACACCAAGAATTGCA+GGG 0.666290 8.4:-32329269 None:intergenic
GGCCATTGATACTGGTTGTG+GGG 0.668748 8.4:+32328762 MS.gene83416:CDS
TTCCAACATTATGAACCGAT+AGG 0.674489 8.4:-32328357 None:intergenic
GATATGAATACGGAATATGG+AGG 0.676561 8.4:+32330039 MS.gene83416:CDS
TGAAGGAGGTCATATGTCCG+AGG 0.677170 8.4:-32330300 None:intergenic
CAATTTCTAAGTGGCCCGAG+CGG 0.677610 8.4:+32329869 MS.gene83416:CDS
AGTCGTGGAACGTTCCAATG+AGG 0.677842 8.4:-32330155 None:intergenic
GGAAGCTGGTACCCATCCGG+AGG 0.678508 8.4:-32328425 None:intergenic
AGAGTCCTGCAAGATGAAGG+AGG 0.687154 8.4:-32330314 None:intergenic
GGCCTCGATCATAAACCACA+GGG 0.703174 8.4:-32330134 None:intergenic
GGGAACATAGTACCTCCACC+AGG 0.712101 8.4:-32328667 None:intergenic
CCACAAATATGATTGCCACA+GGG 0.719216 8.4:-32327798 None:intergenic
ACAGAAGATACCGAGCAGGG+AGG 0.757470 8.4:+32330787 MS.gene83416:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGGTTTAAATATATTTT+TGG + chr8.4:32330399-32330418 MS.gene83416:intron 10.0%
!! AATACTGTTATTAAAACAAA+TGG + chr8.4:32330189-32330208 MS.gene83416:intron 15.0%
!!! AAATTTGTTGATATTGAAAT+TGG + chr8.4:32330481-32330500 MS.gene83416:intron 15.0%
!!! CTGATATTAATTGATTTTTT+TGG - chr8.4:32329071-32329090 None:intergenic 15.0%
!!! GTTACATTTTAATATCTTAT+GGG + chr8.4:32328031-32328050 MS.gene83416:intron 15.0%
!!! TGTTACATTTTAATATCTTA+TGG + chr8.4:32328030-32328049 MS.gene83416:intron 15.0%
!! CCAAGATAATTTATAAAAAC+AGG + chr8.4:32328179-32328198 MS.gene83416:intron 20.0%
!! GTACAAATAACAAAATTAGT+TGG - chr8.4:32329049-32329068 None:intergenic 20.0%
!! TAAAGACTAAAATGATTACA+AGG + chr8.4:32330548-32330567 MS.gene83416:intron 20.0%
!! TTAATATTCTTGAAATACAC+CGG + chr8.4:32329512-32329531 MS.gene83416:CDS 20.0%
!! TTATTGTAACAATCATTAAC+AGG - chr8.4:32329724-32329743 None:intergenic 20.0%
!!! CCTGTTTTTATAAATTATCT+TGG - chr8.4:32328182-32328201 None:intergenic 20.0%
!!! GATTTGAATTTTGTTATGAT+AGG + chr8.4:32330256-32330275 MS.gene83416:intron 20.0%
!!! GTATTTGTACACATTAATTT+TGG + chr8.4:32330382-32330401 MS.gene83416:intron 20.0%
!!! TATAGTTAATTTTACAGAGA+TGG + chr8.4:32328321-32328340 MS.gene83416:intron 20.0%
!!! TATCAGTGTTTTTTTTATCA+CGG + chr8.4:32328810-32328829 MS.gene83416:intron 20.0%
! ATAAGTTGTTTATCCAATCA+GGG + chr8.4:32328292-32328311 MS.gene83416:intron 25.0%
! CTGTTAAAAGTTGCATAAAA+AGG - chr8.4:32329419-32329438 None:intergenic 25.0%
! GATCCAAAGTTTAAGAAAAT+AGG + chr8.4:32329838-32329857 MS.gene83416:CDS 25.0%
! TAATATGCAAGAAAAAGCTA+TGG + chr8.4:32330827-32330846 MS.gene83416:CDS 25.0%
! TATAAGTTGTTTATCCAATC+AGG + chr8.4:32328291-32328310 MS.gene83416:intron 25.0%
! TGATAAGATAAGAAATGTGA+TGG + chr8.4:32330017-32330036 MS.gene83416:CDS 25.0%
! TTCATAGTTACTTGTATTTG+CGG + chr8.4:32330597-32330616 MS.gene83416:intron 25.0%
!! AAAGAGAATTGTAATTTGCA+AGG - chr8.4:32328943-32328962 None:intergenic 25.0%
!! AGACCTATTTTCTTAAACTT+TGG - chr8.4:32329844-32329863 None:intergenic 25.0%
!! ATTTTCTTAAACTTTGGATC+CGG - chr8.4:32329838-32329857 None:intergenic 25.0%
!! CAATATCAACAAATTTTGCA+GGG - chr8.4:32330477-32330496 None:intergenic 25.0%
!! TCAATATCAACAAATTTTGC+AGG - chr8.4:32330478-32330497 None:intergenic 25.0%
!! TTCTGACAATGATTGTTATT+TGG + chr8.4:32330628-32330647 MS.gene83416:intron 25.0%
!!! AAATGTGATGGATATGAATA+CGG + chr8.4:32330029-32330048 MS.gene83416:CDS 25.0%
!!! ACTTATTGATGATGATTTCT+TGG - chr8.4:32327917-32327936 None:intergenic 25.0%
!!! ATTTTAGTCTTTACTTTGAG+GGG - chr8.4:32330541-32330560 None:intergenic 25.0%
!!! CATTTTAGTCTTTACTTTGA+GGG - chr8.4:32330542-32330561 None:intergenic 25.0%
!!! CGGTAAATATGTTGATTTAA+TGG + chr8.4:32328699-32328718 MS.gene83416:CDS 25.0%
!!! CTTATTGATGATGATTTCTT+GGG - chr8.4:32327916-32327935 None:intergenic 25.0%
!!! TCATTTTAGTCTTTACTTTG+AGG - chr8.4:32330543-32330562 None:intergenic 25.0%
!!! TTATTGATGATGATTTCTTG+GGG - chr8.4:32327915-32327934 None:intergenic 25.0%
AAACTATTTGAGAGAGCTTA+TGG + chr8.4:32328103-32328122 MS.gene83416:intron 30.0%
AAATTATCTTGGAGAGCTTA+TGG - chr8.4:32328171-32328190 None:intergenic 30.0%
AACACTGATAAAAACGCAAA+AGG - chr8.4:32328800-32328819 None:intergenic 30.0%
AAGAGCAAAATTCATAGAAG+TGG - chr8.4:32329379-32329398 None:intergenic 30.0%
AGATATAACAGCAAACATGA+CGG - chr8.4:32328560-32328579 None:intergenic 30.0%
ATAGTTCCATCTTTCATTTC+TGG - chr8.4:32328736-32328755 None:intergenic 30.0%
ATGGATATGAATACGGAATA+TGG + chr8.4:32330036-32330055 MS.gene83416:CDS 30.0%
ATGTGGACTTTCTTTCTATA+TGG + chr8.4:32327825-32327844 MS.gene83416:CDS 30.0%
CAGGAAACTTAAAATTATGC+CGG - chr8.4:32328528-32328547 None:intergenic 30.0%
CCATATCGAAAGAATTTGAT+GGG - chr8.4:32329314-32329333 None:intergenic 30.0%
GAAGAATAATGAAGGAAAAG+TGG + chr8.4:32327756-32327775 MS.gene83416:CDS 30.0%
GACGAATGATAATATAAGAC+CGG + chr8.4:32328506-32328525 MS.gene83416:intron 30.0%
GAGCTAGAATATGATTGTTA+AGG - chr8.4:32328966-32328985 None:intergenic 30.0%
GTCTTACATCAATTTCTAAG+TGG + chr8.4:32329860-32329879 MS.gene83416:CDS 30.0%
TAGTTCCATCTTTCATTTCT+GGG - chr8.4:32328735-32328754 None:intergenic 30.0%
TATTTCATAATTACCAGTCG+TGG - chr8.4:32330173-32330192 None:intergenic 30.0%
TGAAATGAAGTACGTATTGT+TGG + chr8.4:32330657-32330676 MS.gene83416:CDS 30.0%
TGATACAATTCTATCGTTCT+CGG - chr8.4:32327866-32327885 None:intergenic 30.0%
TGTGGACTTTCTTTCTATAT+GGG + chr8.4:32327826-32327845 MS.gene83416:CDS 30.0%
TTAATGATCATGTGTCTGTT+TGG + chr8.4:32328074-32328093 MS.gene83416:intron 30.0%
TTCTATATGGGTGTAATCTT+TGG + chr8.4:32327838-32327857 MS.gene83416:CDS 30.0%
! AATATTGCACGTTTTGCTTT+TGG + chr8.4:32330430-32330449 MS.gene83416:intron 30.0%
! ACAAAACTCTTGCTCATTTT+AGG + chr8.4:32329140-32329159 MS.gene83416:intron 30.0%
! CAATCAACATTGGTTGAAAA+AGG + chr8.4:32328624-32328643 MS.gene83416:CDS 30.0%
! CATTCTTTTCTCTCGAAAAT+TGG - chr8.4:32328395-32328414 None:intergenic 30.0%
! TAAAATGAGCAAGAGTTTTG+TGG - chr8.4:32329141-32329160 None:intergenic 30.0%
! TGTTTGTTAATCCAGTCATA+TGG - chr8.4:32328598-32328617 None:intergenic 30.0%
!! TATTGATGATGATTTCTTGG+GGG - chr8.4:32327914-32327933 None:intergenic 30.0%
!!! CTTTTTCAACCAATGTTGAT+TGG - chr8.4:32328626-32328645 None:intergenic 30.0%
AACAGAAGTCCAATCAACAT+TGG + chr8.4:32328614-32328633 MS.gene83416:CDS 35.0%
AACTATACTTAGACCCTGAT+TGG - chr8.4:32328308-32328327 None:intergenic 35.0%
ACCCTTAGCAATAGTTGTAA+TGG - chr8.4:32330749-32330768 None:intergenic 35.0%
CAAAACGTGCAATATTTGCA+AGG - chr8.4:32330426-32330445 None:intergenic 35.0%
CCCATCAAATTCTTTCGATA+TGG + chr8.4:32329311-32329330 MS.gene83416:CDS 35.0%
CTTATTCCATGGACAGAATT+CGG + chr8.4:32329351-32329370 MS.gene83416:CDS 35.0%
GAAAGATGGAACTATTCGAA+CGG + chr8.4:32328741-32328760 MS.gene83416:CDS 35.0%
GAAGTACGTATTGTTGGAAA+TGG + chr8.4:32330663-32330682 MS.gene83416:CDS 35.0%
GATATGAATACGGAATATGG+AGG + chr8.4:32330039-32330058 MS.gene83416:CDS 35.0%
GCCATATCGAAAGAATTTGA+TGG - chr8.4:32329315-32329334 None:intergenic 35.0%
GCCATTACAACTATTGCTAA+GGG + chr8.4:32330745-32330764 MS.gene83416:CDS 35.0%
TCAGAAATGTTCCATATGAC+TGG + chr8.4:32328584-32328603 MS.gene83416:intron 35.0%
TCGAAAGAATTTGATGGGAA+TGG - chr8.4:32329309-32329328 None:intergenic 35.0%
TCTCGTGATAGACAAAAGAA+TGG + chr8.4:32329104-32329123 MS.gene83416:intron 35.0%
TGATAGTCAATACTGCATTC+AGG - chr8.4:32327967-32327986 None:intergenic 35.0%
TGCCATTACAACTATTGCTA+AGG + chr8.4:32330744-32330763 MS.gene83416:CDS 35.0%
TTACAGAGATGGAAGAAGTA+TGG + chr8.4:32328332-32328351 MS.gene83416:intron 35.0%
TTCCAACATTATGAACCGAT+AGG - chr8.4:32328360-32328379 None:intergenic 35.0%
! CAACATTGGTTGAAAAAGGA+AGG + chr8.4:32328628-32328647 MS.gene83416:CDS 35.0%
! CTTGATTGATGATCCTGTAT+GGG + chr8.4:32330074-32330093 MS.gene83416:CDS 35.0%
! CTTTTCTCTCGAAAATTGGA+GGG - chr8.4:32328391-32328410 None:intergenic 35.0%
! TCTTTTCTCTCGAAAATTGG+AGG - chr8.4:32328392-32328411 None:intergenic 35.0%
!! ATCATATTTGTGGTGCTATG+TGG + chr8.4:32327808-32327827 MS.gene83416:CDS 35.0%
!! ATCCTATCGGTTCATAATGT+TGG + chr8.4:32328355-32328374 MS.gene83416:CDS 35.0%
!!! AAGCTTTTTCGACATATCCT+CGG + chr8.4:32330283-32330302 MS.gene83416:CDS 35.0%
!!! ACAGCACTGGTTTTTATGTA+GGG + chr8.4:32328912-32328931 MS.gene83416:intron 35.0%
!!! GTTTTTTTTATCACGGAGCA+CGG + chr8.4:32328817-32328836 MS.gene83416:intron 35.0%
ACATATTTACCGACACCGTT+CGG - chr8.4:32328691-32328710 None:intergenic 40.0%
ACCACAAATATGATTGCCAC+AGG - chr8.4:32327802-32327821 None:intergenic 40.0%
ACCACATTCATAACCCATAC+AGG - chr8.4:32330090-32330109 None:intergenic 40.0%
ACGACAAAACAAGTGCGAAT+TGG - chr8.4:32329814-32329833 None:intergenic 40.0%
ACTGCATTCAGGAAAACTGA+TGG - chr8.4:32327956-32327975 None:intergenic 40.0%
ACTGGATCGTGGAATTCTTA+CGG + chr8.4:32329185-32329204 MS.gene83416:CDS 40.0%
ATGATTTCTTGGGGGAATCA+AGG - chr8.4:32327906-32327925 None:intergenic 40.0%
CAAAGAGCAGAGATGAATGT+TGG + chr8.4:32328459-32328478 MS.gene83416:CDS 40.0%
CAACATTCATCTCTGCTCTT+TGG - chr8.4:32328461-32328480 None:intergenic 40.0%
CACTGCACTAATATTACCCT+TGG - chr8.4:32328012-32328031 None:intergenic 40.0%
CATTAGAATGAGCTGCTTGT+TGG + chr8.4:32330360-32330379 MS.gene83416:intron 40.0%
CCACAAATATGATTGCCACA+GGG - chr8.4:32327801-32327820 None:intergenic 40.0%
CCATACAGGATCATCAATCA+AGG - chr8.4:32330076-32330095 None:intergenic 40.0%
CGAATTCTGTCCATGGAATA+AGG - chr8.4:32329353-32329372 None:intergenic 40.0%
CTGATCCCAGAAATGAAAGA+TGG + chr8.4:32328727-32328746 MS.gene83416:CDS 40.0%
GAAAAGTGGTAGACAAGAGT+AGG + chr8.4:32327770-32327789 MS.gene83416:CDS 40.0%
GAATCTCAATGGTTCGTCAT+GGG + chr8.4:32329911-32329930 MS.gene83416:CDS 40.0%
GCTAGTACTTTCAAACGTGA+CGG + chr8.4:32329937-32329956 MS.gene83416:CDS 40.0%
TAACTACTATTCGACAGCAC+TGG + chr8.4:32328899-32328918 MS.gene83416:intron 40.0%
TACACCAGATCGAATCTCAA+TGG + chr8.4:32329900-32329919 MS.gene83416:CDS 40.0%
TCAAGAATATTAACTCCGCC+TGG - chr8.4:32329505-32329524 None:intergenic 40.0%
TGATTTCTTGGGGGAATCAA+GGG - chr8.4:32327905-32327924 None:intergenic 40.0%
! CCTTGATTGATGATCCTGTA+TGG + chr8.4:32330073-32330092 MS.gene83416:CDS 40.0%
! GGAAGAAGTATGGATCCTAT+CGG + chr8.4:32328342-32328361 MS.gene83416:CDS 40.0%
! GGGACTAGTACAAAACAACA+AGG - chr8.4:32330457-32330476 None:intergenic 40.0%
! TATTGCTAAGGGTATGCGAT+GGG + chr8.4:32330756-32330775 MS.gene83416:CDS 40.0%
! TCACACTGCAATCTTTTACC+AGG + chr8.4:32329484-32329503 MS.gene83416:intron 40.0%
! TCCTGTATGGGTTATGAATG+TGG + chr8.4:32330086-32330105 MS.gene83416:CDS 40.0%
!!! CTCATTTTAGGTTGCTAGCT+GGG + chr8.4:32329152-32329171 MS.gene83416:intron 40.0%
!!! GACAGCACTGGTTTTTATGT+AGG + chr8.4:32328911-32328930 MS.gene83416:intron 40.0%
!!! TCATTTTAGGTTGCTAGCTG+GGG + chr8.4:32329153-32329172 MS.gene83416:intron 40.0%
AAAAAGGCGACATTGCCGTA+TGG + chr8.4:32329689-32329708 MS.gene83416:CDS 45.0%
AAATTCCTCTTTCCTGGTGG+AGG + chr8.4:32328655-32328674 MS.gene83416:CDS 45.0%
AACAGGAGACTTCTGCCATA+CGG - chr8.4:32329707-32329726 None:intergenic 45.0%
AAGAGATAACCACCAAGCTC+AGG + chr8.4:32329221-32329240 MS.gene83416:CDS 45.0%
AAGCGAATTGAACCTGAGCT+TGG - chr8.4:32329236-32329255 None:intergenic 45.0%
AGAATGCTTAGTTCCTCCTC+CGG + chr8.4:32328409-32328428 MS.gene83416:CDS 45.0%
AGCCACCAGGTCGTAAAATT+CGG - chr8.4:32330690-32330709 None:intergenic 45.0%
AGCTCAGGTTCAATTCGCTT+TGG + chr8.4:32329236-32329255 MS.gene83416:CDS 45.0%
ATCTTATCAGTCCCAAGCTC+AGG - chr8.4:32330006-32330025 None:intergenic 45.0%
ATTACCCTTGGATCAGTGCA+TGG - chr8.4:32328000-32328019 None:intergenic 45.0%
ATTCGAACGGCCATTGATAC+TGG + chr8.4:32328754-32328773 MS.gene83416:CDS 45.0%
CAAGGACACCAAGAATTGCA+GGG - chr8.4:32329272-32329291 None:intergenic 45.0%
CATATTTACCGACACCGTTC+GGG - chr8.4:32328690-32328709 None:intergenic 45.0%
CCCTGTGGCAATCATATTTG+TGG + chr8.4:32327798-32327817 MS.gene83416:CDS 45.0%
CGAACCATTGAGATTCGATC+TGG - chr8.4:32329907-32329926 None:intergenic 45.0%
CGAATCTCAATGGTTCGTCA+TGG + chr8.4:32329910-32329929 MS.gene83416:CDS 45.0%
CTGCTCTTTGGCCATCTAAT+TGG - chr8.4:32328449-32328468 None:intergenic 45.0%
GAGAAATTCCTCTTTCCTGG+TGG + chr8.4:32328652-32328671 MS.gene83416:CDS 45.0%
GCAGAGATGAATGTTGGTAC+AGG + chr8.4:32328465-32328484 MS.gene83416:CDS 45.0%
GCTCATTCTAATGTCACCTG+TGG - chr8.4:32330353-32330372 None:intergenic 45.0%
GGAGAGAAATTCCTCTTTCC+TGG + chr8.4:32328649-32328668 MS.gene83416:CDS 45.0%
GGAGTATACCATGCTGAATC+TGG - chr8.4:32329793-32329812 None:intergenic 45.0%
GGTACCAGCTTCCAATTAGA+TGG + chr8.4:32328435-32328454 MS.gene83416:CDS 45.0%
GTCATCGCATTTAGGTGGAT+AGG - chr8.4:32329761-32329780 None:intergenic 45.0%
GTTTATGATCGAGGCCTCAT+TGG + chr8.4:32330141-32330160 MS.gene83416:CDS 45.0%
TATGACCTCCTTCATCTTGC+AGG + chr8.4:32330309-32330328 MS.gene83416:CDS 45.0%
TCAAACGTGACGGCAGTAAT+TGG + chr8.4:32329947-32329966 MS.gene83416:CDS 45.0%
TCTCGAAAATTGGAGGGCAA+TGG - chr8.4:32328385-32328404 None:intergenic 45.0%
TCTTATCAGTCCCAAGCTCA+GGG - chr8.4:32330005-32330024 None:intergenic 45.0%
TGAAGAGTCCTGCAAGATGA+AGG - chr8.4:32330320-32330339 None:intergenic 45.0%
TTGGCCATCTAATTGGAAGC+TGG - chr8.4:32328442-32328461 None:intergenic 45.0%
! AACATGACGGAGTAAGTTGC+AGG - chr8.4:32328547-32328566 None:intergenic 45.0%
! AAGAAGTTGATCCCTGAGCT+TGG + chr8.4:32329991-32330010 MS.gene83416:CDS 45.0%
! AGAAGTTGATCCCTGAGCTT+GGG + chr8.4:32329992-32330011 MS.gene83416:CDS 45.0%
! ATGGACCGAATTTTACGACC+TGG + chr8.4:32330682-32330701 MS.gene83416:CDS 45.0%
! CACTGCAATCTTTTACCAGG+CGG + chr8.4:32329487-32329506 MS.gene83416:intron 45.0%
! CTATTGCTAAGGGTATGCGA+TGG + chr8.4:32330755-32330774 MS.gene83416:CDS 45.0%
! GGAATATGGAGGTTTTGCTG+CGG + chr8.4:32330050-32330069 MS.gene83416:CDS 45.0%
! TCTTCGTCCTGGAGGATTTT+GGG + chr8.4:32329537-32329556 MS.gene83416:CDS 45.0%
! TGAGCTTGGTGGTTATCTCT+TGG - chr8.4:32329222-32329241 None:intergenic 45.0%
! TGTCCATGGAATAAGGCATC+TGG - chr8.4:32329346-32329365 None:intergenic 45.0%
! TTCTTCGTCCTGGAGGATTT+TGG + chr8.4:32329536-32329555 MS.gene83416:CDS 45.0%
!!! GCGCGAGTCTAGTTACTTTT+TGG + chr8.4:32330720-32330739 MS.gene83416:CDS 45.0%
!!! GCTCATTTTAGGTTGCTAGC+TGG + chr8.4:32329151-32329170 MS.gene83416:intron 45.0%
!!! TCGCACTTGTTTTGTCGTTC+CGG + chr8.4:32329816-32329835 MS.gene83416:CDS 45.0%
!! TTTAAATTGTATAATAAAAA+AGG + chr8.4:32329673-32329692 MS.gene83416:CDS 5.0%
ACCATGCTGAATCTGGTGCT+AGG - chr8.4:32329786-32329805 None:intergenic 50.0%
ACTATGTTCCCGAACGGTGT+CGG + chr8.4:32328679-32328698 MS.gene83416:CDS 50.0%
AGAGTCCTGCAAGATGAAGG+AGG - chr8.4:32330317-32330336 None:intergenic 50.0%
AGGCCTCGATCATAAACCAC+AGG - chr8.4:32330138-32330157 None:intergenic 50.0%
AGGCTGTCATCGCATTTAGG+TGG - chr8.4:32329766-32329785 None:intergenic 50.0%
AGTCGTGGAACGTTCCAATG+AGG - chr8.4:32330158-32330177 None:intergenic 50.0%
ATACACCGGATTCTTCGTCC+TGG + chr8.4:32329526-32329545 MS.gene83416:CDS 50.0%
ATCCTCCAGGACGAAGAATC+CGG - chr8.4:32329534-32329553 None:intergenic 50.0%
ATGGAAGCCTGTGTGTAGCA+AGG - chr8.4:32329290-32329309 None:intergenic 50.0%
ATTGGAAGCTGGTACCCATC+CGG - chr8.4:32328431-32328450 None:intergenic 50.0%
CAAGAGTAGGATTGTCCCTG+TGG + chr8.4:32327783-32327802 MS.gene83416:CDS 50.0%
CAATTTCTAAGTGGCCCGAG+CGG + chr8.4:32329869-32329888 MS.gene83416:CDS 50.0%
CACCCCACAACCAGTATCAA+TGG - chr8.4:32328767-32328786 None:intergenic 50.0%
CGAATTGAACCTGAGCTTGG+TGG - chr8.4:32329233-32329252 None:intergenic 50.0%
CGTACAGAAGATACCGAGCA+GGG + chr8.4:32330784-32330803 MS.gene83416:CDS 50.0%
CGTGGTATCCCTGCAATTCT+TGG + chr8.4:32329261-32329280 MS.gene83416:CDS 50.0%
CTCCCTGTGGTTTATGATCG+AGG + chr8.4:32330132-32330151 MS.gene83416:CDS 50.0%
GACAAGACCCAAAATCCTCC+AGG - chr8.4:32329547-32329566 None:intergenic 50.0%
GATCTGCGCTCGTAGTTGAT+TGG - chr8.4:32329577-32329596 None:intergenic 50.0%
GCAAGGACACCAAGAATTGC+AGG - chr8.4:32329273-32329292 None:intergenic 50.0%
GCTAGGCTGTCATCGCATTT+AGG - chr8.4:32329769-32329788 None:intergenic 50.0%
GGCCTCGATCATAAACCACA+GGG - chr8.4:32330137-32330156 None:intergenic 50.0%
GGTGGTGATTTACTGGATCG+TGG + chr8.4:32329174-32329193 MS.gene83416:CDS 50.0%
TAGCTGGGGTGGTGATTTAC+TGG + chr8.4:32329167-32329186 MS.gene83416:CDS 50.0%
TAGTACCTCCACCAGGAAAG+AGG - chr8.4:32328663-32328682 None:intergenic 50.0%
TCACGGAGCACGGAAAATAG+CGG + chr8.4:32328827-32328846 MS.gene83416:intron 50.0%
TCATTGGAACGTTCCACGAC+TGG + chr8.4:32330157-32330176 MS.gene83416:CDS 50.0%
TCGTACAGAAGATACCGAGC+AGG + chr8.4:32330783-32330802 MS.gene83416:CDS 50.0%
TCTTCACTGCAGAAAGCCAC+AGG + chr8.4:32330334-32330353 MS.gene83416:CDS 50.0%
TGAAGGAGGTCATATGTCCG+AGG - chr8.4:32330303-32330322 None:intergenic 50.0%
TGCTGTGAATACACTCCCTG+TGG + chr8.4:32330119-32330138 MS.gene83416:CDS 50.0%
TGGTGTAACATGCAACCGCT+CGG - chr8.4:32329887-32329906 None:intergenic 50.0%
! ACACTCCATGCACTGATCCA+AGG + chr8.4:32327992-32328011 MS.gene83416:CDS 50.0%
! ACGGCCATTGATACTGGTTG+TGG + chr8.4:32328760-32328779 MS.gene83416:CDS 50.0%
! CACTCCATGCACTGATCCAA+GGG + chr8.4:32327993-32328012 MS.gene83416:CDS 50.0%
! CGGCCATTGATACTGGTTGT+GGG + chr8.4:32328761-32328780 MS.gene83416:CDS 50.0%
! CTCGTAGTTGATTGGTGGAC+CGG - chr8.4:32329569-32329588 None:intergenic 50.0%
! GACCGAATTTTACGACCTGG+TGG + chr8.4:32330685-32330704 MS.gene83416:CDS 50.0%
! GCTCCAGATGCCTTATTCCA+TGG + chr8.4:32329340-32329359 MS.gene83416:CDS 50.0%
! GGCCATTGATACTGGTTGTG+GGG + chr8.4:32328762-32328781 MS.gene83416:CDS 50.0%
! GTGGTACCGAATTCTGTCCA+TGG - chr8.4:32329360-32329379 None:intergenic 50.0%
! TTGGTGTCCTTGCTACACAC+AGG + chr8.4:32329280-32329299 MS.gene83416:CDS 50.0%
!! GGAGGATTTTGGGTCTTGTC+CGG + chr8.4:32329547-32329566 MS.gene83416:CDS 50.0%
!! GGAGGTACTATGTTCCCGAA+CGG + chr8.4:32328673-32328692 MS.gene83416:CDS 50.0%
!! GTTCAATTCGCTTTGGAGCG+TGG + chr8.4:32329243-32329262 MS.gene83416:CDS 50.0%
!!! TTTTAGGTTGCTAGCTGGGG+TGG + chr8.4:32329156-32329175 MS.gene83416:intron 50.0%
ACAGAAGATACCGAGCAGGG+AGG + chr8.4:32330787-32330806 MS.gene83416:CDS 55.0%
CTGCGCTCGTAGTTGATTGG+TGG - chr8.4:32329574-32329593 None:intergenic 55.0%
GCCTAGCACCAGATTCAGCA+TGG + chr8.4:32329782-32329801 MS.gene83416:CDS 55.0%
GCTTAGTTCCTCCTCCGGAT+GGG + chr8.4:32328414-32328433 MS.gene83416:CDS 55.0%
GGGAACATAGTACCTCCACC+AGG - chr8.4:32328670-32328689 None:intergenic 55.0%
GGTGTAACATGCAACCGCTC+GGG - chr8.4:32329886-32329905 None:intergenic 55.0%
TGCTTAGTTCCTCCTCCGGA+TGG + chr8.4:32328413-32328432 MS.gene83416:CDS 55.0%
TGGGGGAATCAAGGGATCTC+TGG - chr8.4:32327897-32327916 None:intergenic 55.0%
AACTACGAGCGCAGATCGCG+TGG + chr8.4:32329580-32329599 MS.gene83416:CDS 60.0%
CACCGGATTCTTCGTCCTGG+AGG + chr8.4:32329529-32329548 MS.gene83416:CDS 60.0%
CTCCATGTCACCTCCCTGCT+CGG - chr8.4:32330800-32330819 None:intergenic 60.0%
TACCGAGCAGGGAGGTGACA+TGG + chr8.4:32330795-32330814 MS.gene83416:CDS 60.0%
ACGAGCGCAGATCGCGTGGA+TGG + chr8.4:32329584-32329603 MS.gene83416:CDS 65.0%
AGCTGGTACCCATCCGGAGG+AGG - chr8.4:32328425-32328444 None:intergenic 65.0%
GGAAGCTGGTACCCATCCGG+AGG - chr8.4:32328428-32328447 None:intergenic 65.0%
! CGCACGAGAGCATAGCCACC+AGG - chr8.4:32330703-32330722 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 32327754 32330876 32327754 ID=MS.gene83416
chr8.4 mRNA 32327754 32330876 32327754 ID=MS.gene83416.t1;Parent=MS.gene83416
chr8.4 exon 32327754 32328014 32327754 ID=MS.gene83416.t1.exon1;Parent=MS.gene83416.t1
chr8.4 CDS 32327754 32328014 32327754 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
chr8.4 exon 32328338 32328486 32328338 ID=MS.gene83416.t1.exon2;Parent=MS.gene83416.t1
chr8.4 CDS 32328338 32328486 32328338 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
chr8.4 exon 32328588 32328783 32328588 ID=MS.gene83416.t1.exon3;Parent=MS.gene83416.t1
chr8.4 CDS 32328588 32328783 32328588 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
chr8.4 exon 32329162 32329372 32329162 ID=MS.gene83416.t1.exon4;Parent=MS.gene83416.t1
chr8.4 CDS 32329162 32329372 32329162 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
chr8.4 exon 32329506 32330178 32329506 ID=MS.gene83416.t1.exon5;Parent=MS.gene83416.t1
chr8.4 CDS 32329506 32330178 32329506 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
chr8.4 exon 32330278 32330355 32330278 ID=MS.gene83416.t1.exon6;Parent=MS.gene83416.t1
chr8.4 CDS 32330278 32330355 32330278 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
chr8.4 exon 32330654 32330876 32330654 ID=MS.gene83416.t1.exon7;Parent=MS.gene83416.t1
chr8.4 CDS 32330654 32330876 32330654 ID=cds.MS.gene83416.t1;Parent=MS.gene83416.t1
Gene Sequence

>MS.gene83416

ATGAAGAATAATGAAGGAAAAGTGGTAGACAAGAGTAGGATTGTCCCTGTGGCAATCATATTTGTGGTGCTATGTGGACTTTCTTTCTATATGGGTGTAATCTTTGGTTCCGAGAACGATAGAATTGTATCAATTATCAGCCAGAGATCCCTTGATTCCCCCAAGAAATCATCATCAATAAGTTCTTTGCAAATTAAATCCATCAGTTTTCCTGAATGCAGTATTGACTATCAAGATTACACTCCATGCACTGATCCAAGGGTAATATTAGTGCAGTGTTACATTTTAATATCTTATGGGATTTGTAGAAGAAAGTTTTCTTAATGATCATGTGTCTGTTTGGATCGTAAAACTATTTGAGAGAGCTTATGGAAACAACTTATGATATGTCTATAAGTTGTTTTCAGTTTATTTCCATAAGCTCTCCAAGATAATTTATAAAAACAGGCTATATCTTGTATAAAAACAATTTGTCTTTATTTTATCTTTTATTATAGATATAGCTTATTCACAATCATTTACAATAAGCTCTTATGCTATAAGTTGTTTATCCAATCAGGGTCTAAGTATAGTTAATTTTACAGAGATGGAAGAAGTATGGATCCTATCGGTTCATAATGTTGGAACGCCATTGCCCTCCAATTTTCGAGAGAAAAGAATGCTTAGTTCCTCCTCCGGATGGGTACCAGCTTCCAATTAGATGGCCAAAGAGCAGAGATGAATGTTGGTACAGGTAATTATATCAGTCTGACGACGAATGATAATATAAGACCGGCATAATTTTAAGTTTCCTGCAACTTACTCCGTCATGTTTGCTGTTATATCTAATTTCAGAAATGTTCCATATGACTGGATTAACAAACAGAAGTCCAATCAACATTGGTTGAAAAAGGAAGGAGAGAAATTCCTCTTTCCTGGTGGAGGTACTATGTTCCCGAACGGTGTCGGTAAATATGTTGATTTAATGGAAGCTCTGATCCCAGAAATGAAAGATGGAACTATTCGAACGGCCATTGATACTGGTTGTGGGGTGAGTCTTGCTTCCTTTTGCGTTTTTATCAGTGTTTTTTTTATCACGGAGCACGGAAAATAGCGGTATGCTCAAACTCTGCTACACTACAGTCTACAGCGCTATAGTATCGCTATAACTACTATTCGACAGCACTGGTTTTTATGTAGGGAATAGCCTTGCAAATTACAATTCTCTTTCCTTAACAATCATATTCTAGCTCAATTATATGTGCTTCTTCTTGCAGTTTGCTTTTCTCACTGCATTGTAATATATTTTAAAACCAACTAATTTTGTTATTTGTACCAAAAAAATCAATTAATATCAGTTGCAATTTTCAATCTCGTGATAGACAAAAGAATGGCATCAATTAATCCACAAAACTCTTGCTCATTTTAGGTTGCTAGCTGGGGTGGTGATTTACTGGATCGTGGAATTCTTACGGTTTCTCTTGCTCCAAGAGATAACCACCAAGCTCAGGTTCAATTCGCTTTGGAGCGTGGTATCCCTGCAATTCTTGGTGTCCTTGCTACACACAGGCTTCCATTCCCATCAAATTCTTTCGATATGGCTCATTGCTCCAGATGCCTTATTCCATGGACAGAATTCGGTACCACTTCTATGAATTTTGCTCTTAATCAAACTGAATTAATCCTTTTTATGCAACTTTTAACAGTGATCATGAACTTTACAAGTCTTGTAGCTTCTTAAACTTTGAAATTCACACTGCAATCTTTTACCAGGCGGAGTTAATATTCTTGAAATACACCGGATTCTTCGTCCTGGAGGATTTTGGGTCTTGTCCGGTCCACCAATCAACTACGAGCGCAGATCGCGTGGATGGAACACAACTATCGAAGAGCAAAGATCAACTTACGAGAAATTGCAGAACTTACTGACTTCATTGTGCTTTAAATTGTATAATAAAAAAGGCGACATTGCCGTATGGCAGAAGTCTCCTGTTAATGATTGTTACAATAAGCTGACTAGAGATACCTATCCACCTAAATGCGATGACAGCCTAGCACCAGATTCAGCATGGTATACTCCAATTCGCACTTGTTTTGTCGTTCCGGATCCAAAGTTTAAGAAAATAGGTCTTACATCAATTTCTAAGTGGCCCGAGCGGTTGCATGTTACACCAGATCGAATCTCAATGGTTCGTCATGGGAGTGCTAGTACTTTCAAACGTGACGGCAGTAATTGGAAGAAGCATGTCGCACACTACAAGAAGTTGATCCCTGAGCTTGGGACTGATAAGATAAGAAATGTGATGGATATGAATACGGAATATGGAGGTTTTGCTGCGGCCTTGATTGATGATCCTGTATGGGTTATGAATGTGGTCTCATCTTATGCTGTGAATACACTCCCTGTGGTTTATGATCGAGGCCTCATTGGAACGTTCCACGACTGGTAATTATGAAATACTGTTATTAAAACAAATGGTTTCATTTCTGTATATAATTTTGTCTTATAAATGATCTACTGTTGATTTGAATTTTGTTATGATAGGTGTGAAGCTTTTTCGACATATCCTCGGACATATGACCTCCTTCATCTTGCAGGACTCTTCACTGCAGAAAGCCACAGGTGACATTAGAATGAGCTGCTTGTTGGTATTTGTACACATTAATTTTGGTTTAAATATATTTTTGGTCCTTGCAAATATTGCACGTTTTGCTTTTGGTCCTTGTTGTTTTGTACTAGTCCCTGCAAAATTTGTTGATATTGAAATTGGTGTTTGTAACATGTAAAATGTATTTGATTTTAGTCCCCTCAAAGTAAAGACTAAAATGATTACAAGGCTAATTTGATAAGTGATGATACTTGTTTCATAGTTACTTGTATTTGCGGCTGCTTGATTCTGACAATGATTGTTATTTGGCAGATGTGAAATGAAGTACGTATTGTTGGAAATGGACCGAATTTTACGACCTGGTGGCTATGCTCTCGTGCGCGAGTCTAGTTACTTTTTGGATGCCATTACAACTATTGCTAAGGGTATGCGATGGGAATGTCGTACAGAAGATACCGAGCAGGGAGGTGACATGGAGAAGATACTAATATGCAAGAAAAAGCTATGGTATTCATCAAATAAAGATTCAAGATGA
Protein sequence

>MS.gene83416.t1

MKNNEGKVVDKSRIVPVAIIFVVLCGLSFYMGVIFGSENDRIVSIISQRSLDSPKKSSSISSLQIKSISFPECSIDYQDYTPCTDPRRWKKYGSYRFIMLERHCPPIFERKECLVPPPDGYQLPIRWPKSRDECWYRNVPYDWINKQKSNQHWLKKEGEKFLFPGGGTMFPNGVGKYVDLMEALIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHQAQVQFALERGIPAILGVLATHRLPFPSNSFDMAHCSRCLIPWTEFGGVNILEIHRILRPGGFWVLSGPPINYERRSRGWNTTIEEQRSTYEKLQNLLTSLCFKLYNKKGDIAVWQKSPVNDCYNKLTRDTYPPKCDDSLAPDSAWYTPIRTCFVVPDPKFKKIGLTSISKWPERLHVTPDRISMVRHGSASTFKRDGSNWKKHVAHYKKLIPELGTDKIRNVMDMNTEYGGFAAALIDDPVWVMNVVSSYAVNTLPVVYDRGLIGTFHDWCEAFSTYPRTYDLLHLAGLFTAESHRCEMKYVLLEMDRILRPGGYALVRESSYFLDAITTIAKGMRWECRTEDTEQGGDMEKILICKKKLWYSSNKDSR