Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85361.t1 | GAU28374.1 | 48.8 | 82 | 5 | 1 | 2 | 46 | 361 | 442 | 6.80E-08 | 65.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85361.t1 | Q9LK65 | 43.9 | 82 | 9 | 1 | 2 | 46 | 386 | 467 | 1.4e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85361.t1 | G7K4G4 | 48.8 | 82 | 5 | 1 | 2 | 46 | 393 | 474 | 4.9e-08 | 65.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene85361 | 0.809637 | 1.71E-50 | -1.69E-46 |
| MS.gene049853 | MS.gene85361 | 0.849787 | 2.58E-60 | -1.69E-46 |
| MS.gene054174 | MS.gene85361 | 0.828768 | 7.48E-55 | -1.69E-46 |
| MS.gene054175 | MS.gene85361 | 0.834748 | 2.50E-56 | -1.69E-46 |
| MS.gene054484 | MS.gene85361 | 0.813043 | 3.13E-51 | -1.69E-46 |
| MS.gene055058 | MS.gene85361 | 0.814207 | 1.73E-51 | -1.69E-46 |
| MS.gene059276 | MS.gene85361 | 0.819873 | 9.24E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATATGCGACGGATCTGC+TGG | 0.409302 | 2.1:+47188385 | MS.gene85361:CDS |
| ATGAGTATGGTGGAACTGTT+GGG | 0.464601 | 2.1:+47188769 | MS.gene85361:CDS |
| GCTGTCGTTCTTAATGAGTA+TGG | 0.485683 | 2.1:+47188756 | MS.gene85361:CDS |
| AATGAGTATGGTGGAACTGT+TGG | 0.522457 | 2.1:+47188768 | MS.gene85361:CDS |
| GCAACATGTTGATAATATGA+TGG | 0.547277 | 2.1:+47188358 | MS.gene85361:CDS |
| TCTGCTGGATTATGTCCAAA+AGG | 0.558858 | 2.1:+47188400 | MS.gene85361:CDS |
| CAACATGTTGATAATATGAT+GGG | 0.620657 | 2.1:+47188359 | MS.gene85361:CDS |
| GTCGTTCTTAATGAGTATGG+TGG | 0.629139 | 2.1:+47188759 | MS.gene85361:CDS |
| TTGAATCTGAAAGGTTCACA+TGG | 0.656775 | 2.1:+47188734 | MS.gene85361:intron |
| GATGGGTATAGCATATGCGA+CGG | 0.657260 | 2.1:+47188376 | MS.gene85361:CDS |
| TGAGTATGGTGGAACTGTTG+GGG | 0.713650 | 2.1:+47188770 | MS.gene85361:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGACATAAATATGAATATA+TGG | + | chr2.1:47188839-47188858 | MS.gene85361:intron | 15.0% |
| !! | AACAATGAGATTGAATTAAT+AGG | - | chr2.1:47188473-47188492 | None:intergenic | 20.0% |
| ! | TCGAAATTGAATTGTTAAAC+TGG | + | chr2.1:47188611-47188630 | MS.gene85361:intron | 25.0% |
| ! | TTAAACTGGTTGAATTGTTT+GGG | + | chr2.1:47188625-47188644 | MS.gene85361:intron | 25.0% |
| !!! | CAACATGTTGATAATATGAT+GGG | + | chr2.1:47188359-47188378 | MS.gene85361:CDS | 25.0% |
| AAGTACCATAATTGGAGATA+TGG | + | chr2.1:47188537-47188556 | MS.gene85361:intron | 30.0% | |
| AATGTCTGTTTGAATCTGAA+AGG | + | chr2.1:47188725-47188744 | MS.gene85361:intron | 30.0% | |
| GCATCAATATCTGTTTAACA+TGG | + | chr2.1:47188656-47188675 | MS.gene85361:intron | 30.0% | |
| GGAGATTTCACTACTATTAT+TGG | + | chr2.1:47188426-47188445 | MS.gene85361:intron | 30.0% | |
| GTTAAACTGGTTGAATTGTT+TGG | + | chr2.1:47188624-47188643 | MS.gene85361:intron | 30.0% | |
| TTACCAGGTAAAAAATATGC+AGG | - | chr2.1:47188571-47188590 | None:intergenic | 30.0% | |
| TTTAACATGGTGCGTAATAT+TGG | + | chr2.1:47188669-47188688 | MS.gene85361:intron | 30.0% | |
| ! | AGATTGGTTGAATTCTAGAA+GGG | - | chr2.1:47188806-47188825 | None:intergenic | 30.0% |
| !! | ACTCTAGAAAGTACCATAAT+TGG | + | chr2.1:47188529-47188548 | MS.gene85361:intron | 30.0% |
| !! | GCAACATGTTGATAATATGA+TGG | + | chr2.1:47188358-47188377 | MS.gene85361:CDS | 30.0% |
| AACAGACATTGAATCTGCAA+AGG | - | chr2.1:47188715-47188734 | None:intergenic | 35.0% | |
| GTAAGCCATATCTCCAATTA+TGG | - | chr2.1:47188545-47188564 | None:intergenic | 35.0% | |
| TAAGATGAAGTCAAGTTACC+AGG | - | chr2.1:47188586-47188605 | None:intergenic | 35.0% | |
| TCAACATGTTGCTCAAAAAC+AGG | - | chr2.1:47188350-47188369 | None:intergenic | 35.0% | |
| TGGATTATGTCCAAAAGGTT+GGG | + | chr2.1:47188405-47188424 | MS.gene85361:intron | 35.0% | |
| TTGAATCTGAAAGGTTCACA+TGG | + | chr2.1:47188734-47188753 | MS.gene85361:intron | 35.0% | |
| ! | CAAACAAACTGATCTGAGAT+TGG | - | chr2.1:47188822-47188841 | None:intergenic | 35.0% |
| ! | CACTACTATTATTGGTTGCA+AGG | + | chr2.1:47188434-47188453 | MS.gene85361:intron | 35.0% |
| ! | GAGATTGGTTGAATTCTAGA+AGG | - | chr2.1:47188807-47188826 | None:intergenic | 35.0% |
| !!! | AAGCCTGCATATTTTTTACC+TGG | + | chr2.1:47188565-47188584 | MS.gene85361:intron | 35.0% |
| CTGGATTATGTCCAAAAGGT+TGG | + | chr2.1:47188404-47188423 | MS.gene85361:intron | 40.0% | |
| GCTGTCGTTCTTAATGAGTA+TGG | + | chr2.1:47188756-47188775 | MS.gene85361:CDS | 40.0% | |
| GTCGTTCTTAATGAGTATGG+TGG | + | chr2.1:47188759-47188778 | MS.gene85361:CDS | 40.0% | |
| TCTGCTGGATTATGTCCAAA+AGG | + | chr2.1:47188400-47188419 | MS.gene85361:CDS | 40.0% | |
| TGGTTGAATTGTTTGGGCTA+TGG | + | chr2.1:47188631-47188650 | MS.gene85361:intron | 40.0% | |
| ! | AATGAGTATGGTGGAACTGT+TGG | + | chr2.1:47188768-47188787 | MS.gene85361:CDS | 40.0% |
| ! | AGTGAAATCTCCCAACCTTT+TGG | - | chr2.1:47188418-47188437 | None:intergenic | 40.0% |
| ! | ATGAGTATGGTGGAACTGTT+GGG | + | chr2.1:47188769-47188788 | MS.gene85361:CDS | 40.0% |
| GATGGGTATAGCATATGCGA+CGG | + | chr2.1:47188376-47188395 | MS.gene85361:CDS | 45.0% | |
| ! | TGAGTATGGTGGAACTGTTG+GGG | + | chr2.1:47188770-47188789 | MS.gene85361:CDS | 45.0% |
| AGCATATGCGACGGATCTGC+TGG | + | chr2.1:47188385-47188404 | MS.gene85361:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 47188332 | 47188904 | 47188332 | ID=MS.gene85361 |
| chr2.1 | mRNA | 47188332 | 47188904 | 47188332 | ID=MS.gene85361.t1;Parent=MS.gene85361 |
| chr2.1 | exon | 47188332 | 47188421 | 47188332 | ID=MS.gene85361.t1.exon1;Parent=MS.gene85361.t1 |
| chr2.1 | CDS | 47188332 | 47188421 | 47188332 | ID=cds.MS.gene85361.t1;Parent=MS.gene85361.t1 |
| chr2.1 | exon | 47188747 | 47188791 | 47188747 | ID=MS.gene85361.t1.exon2;Parent=MS.gene85361.t1 |
| chr2.1 | CDS | 47188747 | 47188791 | 47188747 | ID=cds.MS.gene85361.t1;Parent=MS.gene85361.t1 |
| chr2.1 | exon | 47188875 | 47188904 | 47188875 | ID=MS.gene85361.t1.exon3;Parent=MS.gene85361.t1 |
| chr2.1 | CDS | 47188875 | 47188904 | 47188875 | ID=cds.MS.gene85361.t1;Parent=MS.gene85361.t1 |
| Gene Sequence |
| Protein sequence |
