Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86156.t1 | XP_013451066.1 | 96.8 | 126 | 4 | 0 | 1 | 126 | 980 | 1105 | 5.00E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86156.t1 | A0A072U6U6 | 96.8 | 126 | 4 | 0 | 1 | 126 | 980 | 1105 | 3.6e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050026 | MS.gene86156 | 0.816588 | 5.12E-52 | -1.69E-46 |
| MS.gene051492 | MS.gene86156 | 0.821598 | 3.71E-53 | -1.69E-46 |
| MS.gene052447 | MS.gene86156 | 0.828494 | 8.71E-55 | -1.69E-46 |
| MS.gene05414 | MS.gene86156 | 0.803216 | 3.87E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene86156 | 0.822809 | 1.94E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene86156 | -0.81716 | 3.81E-52 | -1.69E-46 |
| MS.gene05435 | MS.gene86156 | 0.839768 | 1.30E-57 | -1.69E-46 |
| MS.gene057026 | MS.gene86156 | 0.808293 | 3.33E-50 | -1.69E-46 |
| MS.gene05925 | MS.gene86156 | 0.836731 | 7.88E-57 | -1.69E-46 |
| MS.gene059459 | MS.gene86156 | 0.801325 | 9.50E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene86156 | 0.812097 | 5.03E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86156.t1 | MTR_6g015665 | 96.875 | 128 | 4 | 0 | 1 | 128 | 980 | 1107 | 1.09e-79 | 255 |
| MS.gene86156.t1 | MTR_6g015595 | 80.620 | 129 | 25 | 0 | 1 | 129 | 1 | 129 | 2.83e-72 | 216 |
| MS.gene86156.t1 | MTR_6g015505 | 80.620 | 129 | 25 | 0 | 1 | 129 | 703 | 831 | 7.74e-67 | 218 |
| MS.gene86156.t1 | MTR_6g016000 | 76.923 | 130 | 30 | 0 | 1 | 130 | 971 | 1100 | 2.50e-64 | 212 |
| MS.gene86156.t1 | MTR_6g016000 | 76.923 | 130 | 30 | 0 | 1 | 130 | 953 | 1082 | 2.66e-64 | 212 |
| MS.gene86156.t1 | MTR_3g104130 | 72.093 | 129 | 35 | 1 | 1 | 129 | 1 | 128 | 9.50e-64 | 193 |
| MS.gene86156.t1 | MTR_3g104210 | 72.093 | 129 | 35 | 1 | 1 | 129 | 1 | 128 | 9.50e-64 | 193 |
| MS.gene86156.t1 | MTR_6g015455 | 77.519 | 129 | 29 | 0 | 1 | 129 | 1270 | 1398 | 3.79e-63 | 209 |
| MS.gene86156.t1 | MTR_6g015455 | 78.740 | 127 | 26 | 1 | 1 | 127 | 287 | 412 | 4.07e-62 | 206 |
| MS.gene86156.t1 | MTR_6g015765 | 73.810 | 126 | 32 | 1 | 1 | 126 | 1 | 125 | 9.42e-63 | 190 |
| MS.gene86156.t1 | MTR_6g015430 | 67.320 | 153 | 45 | 2 | 1 | 149 | 747 | 898 | 1.44e-62 | 206 |
| MS.gene86156.t1 | MTR_3g104170 | 71.318 | 129 | 36 | 1 | 1 | 129 | 1 | 128 | 1.65e-62 | 190 |
| MS.gene86156.t1 | MTR_3g104240 | 71.318 | 129 | 36 | 1 | 1 | 129 | 1 | 128 | 1.65e-62 | 190 |
| MS.gene86156.t1 | MTR_6g015430 | 67.320 | 153 | 45 | 2 | 1 | 149 | 940 | 1091 | 2.52e-62 | 206 |
| MS.gene86156.t1 | MTR_7g091540 | 73.643 | 129 | 33 | 1 | 1 | 129 | 536 | 663 | 4.79e-62 | 202 |
| MS.gene86156.t1 | MTR_6g015435 | 77.953 | 127 | 27 | 1 | 1 | 127 | 905 | 1030 | 1.26e-61 | 204 |
| MS.gene86156.t1 | MTR_6g015490 | 73.643 | 129 | 33 | 1 | 1 | 129 | 747 | 874 | 3.77e-61 | 202 |
| MS.gene86156.t1 | MTR_6g015490 | 73.643 | 129 | 33 | 1 | 1 | 129 | 973 | 1100 | 6.32e-61 | 202 |
| MS.gene86156.t1 | MTR_6g015680 | 72.868 | 129 | 34 | 1 | 1 | 129 | 932 | 1059 | 1.35e-60 | 201 |
| MS.gene86156.t1 | MTR_6g015675 | 72.441 | 127 | 34 | 1 | 1 | 127 | 343 | 468 | 6.23e-60 | 192 |
| MS.gene86156.t1 | MTR_6g015695 | 72.868 | 129 | 34 | 1 | 1 | 129 | 931 | 1058 | 8.47e-59 | 196 |
| MS.gene86156.t1 | MTR_6g015730 | 72.222 | 126 | 34 | 1 | 1 | 126 | 1204 | 1328 | 9.66e-58 | 193 |
| MS.gene86156.t1 | MTR_6g015510 | 73.438 | 128 | 33 | 1 | 1 | 128 | 919 | 1045 | 2.21e-56 | 189 |
| MS.gene86156.t1 | MTR_6g015775 | 70.492 | 122 | 35 | 1 | 7 | 128 | 1001 | 1121 | 3.66e-56 | 189 |
| MS.gene86156.t1 | MTR_6g015405 | 78.182 | 110 | 24 | 0 | 20 | 129 | 947 | 1056 | 3.94e-54 | 183 |
| MS.gene86156.t1 | MTR_6g015745 | 67.442 | 129 | 41 | 1 | 1 | 129 | 921 | 1048 | 5.04e-54 | 182 |
| MS.gene86156.t1 | MTR_4g014990 | 68.217 | 129 | 38 | 2 | 1 | 129 | 924 | 1049 | 1.08e-51 | 176 |
| MS.gene86156.t1 | MTR_6g015410 | 71.429 | 126 | 35 | 1 | 4 | 129 | 897 | 1021 | 1.67e-51 | 175 |
| MS.gene86156.t1 | MTR_6g015600 | 67.969 | 128 | 36 | 3 | 1 | 128 | 625 | 747 | 2.65e-50 | 171 |
| MS.gene86156.t1 | MTR_0294s0010 | 66.667 | 123 | 39 | 2 | 7 | 129 | 728 | 848 | 1.17e-49 | 170 |
| MS.gene86156.t1 | MTR_4g015030 | 64.062 | 128 | 44 | 2 | 4 | 130 | 946 | 1072 | 4.75e-49 | 168 |
| MS.gene86156.t1 | MTR_4g014570 | 62.698 | 126 | 46 | 1 | 4 | 129 | 967 | 1091 | 5.64e-48 | 165 |
| MS.gene86156.t1 | MTR_2g450830 | 65.833 | 120 | 36 | 1 | 8 | 127 | 751 | 865 | 7.96e-47 | 162 |
| MS.gene86156.t1 | MTR_7g055970 | 64.000 | 125 | 41 | 3 | 4 | 127 | 364 | 485 | 8.36e-46 | 156 |
| MS.gene86156.t1 | MTR_4g015060 | 63.780 | 127 | 45 | 1 | 4 | 130 | 939 | 1064 | 3.50e-45 | 157 |
| MS.gene86156.t1 | MTR_4g068200 | 62.121 | 132 | 46 | 3 | 1 | 129 | 470 | 600 | 6.74e-45 | 155 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1404 | 1528 | 9.90e-45 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1403 | 1527 | 1.05e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1369 | 1493 | 1.09e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1403 | 1527 | 1.10e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1905 | 2029 | 1.13e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1905 | 2029 | 1.13e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1369 | 1493 | 1.14e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1871 | 1995 | 1.17e-44 | 156 |
| MS.gene86156.t1 | MTR_7g088950 | 64.062 | 128 | 42 | 3 | 1 | 127 | 1369 | 1493 | 1.20e-44 | 156 |
| MS.gene86156.t1 | MTR_4g023400 | 61.832 | 131 | 46 | 3 | 1 | 130 | 1398 | 1525 | 1.38e-44 | 155 |
| MS.gene86156.t1 | MTR_6g015580 | 55.333 | 150 | 44 | 4 | 10 | 146 | 739 | 878 | 3.69e-42 | 149 |
| MS.gene86156.t1 | MTR_6g015580 | 55.333 | 150 | 44 | 4 | 10 | 146 | 905 | 1044 | 4.07e-42 | 149 |
| MS.gene86156.t1 | MTR_8g037220 | 53.846 | 130 | 60 | 0 | 4 | 133 | 810 | 939 | 3.14e-41 | 146 |
| MS.gene86156.t1 | MTR_8g037220 | 53.846 | 130 | 60 | 0 | 4 | 133 | 589 | 718 | 3.44e-41 | 146 |
| MS.gene86156.t1 | MTR_4g020535 | 52.419 | 124 | 56 | 1 | 7 | 127 | 870 | 993 | 5.86e-37 | 134 |
| MS.gene86156.t1 | MTR_4g020590 | 50.833 | 120 | 59 | 0 | 10 | 129 | 1395 | 1514 | 1.85e-36 | 132 |
| MS.gene86156.t1 | MTR_4g020590 | 50.833 | 120 | 59 | 0 | 10 | 129 | 1388 | 1507 | 1.96e-36 | 132 |
| MS.gene86156.t1 | MTR_8g012005 | 48.387 | 124 | 64 | 0 | 4 | 127 | 15 | 138 | 1.02e-35 | 125 |
| MS.gene86156.t1 | MTR_3g072140 | 46.825 | 126 | 67 | 0 | 4 | 129 | 703 | 828 | 2.00e-35 | 129 |
| MS.gene86156.t1 | MTR_6g465280 | 70.513 | 78 | 23 | 0 | 46 | 123 | 1 | 78 | 2.08e-35 | 119 |
| MS.gene86156.t1 | MTR_8g011850 | 44.521 | 146 | 72 | 2 | 4 | 148 | 910 | 1047 | 2.69e-35 | 129 |
| MS.gene86156.t1 | MTR_8g012180 | 44.928 | 138 | 76 | 0 | 10 | 147 | 672 | 809 | 5.31e-35 | 128 |
| MS.gene86156.t1 | MTR_8g012180 | 44.928 | 138 | 76 | 0 | 10 | 147 | 881 | 1018 | 6.54e-35 | 128 |
| MS.gene86156.t1 | MTR_8g012080 | 45.528 | 123 | 67 | 0 | 7 | 129 | 882 | 1004 | 8.68e-35 | 127 |
| MS.gene86156.t1 | MTR_8g073730 | 46.212 | 132 | 71 | 0 | 6 | 137 | 882 | 1013 | 1.31e-34 | 127 |
| MS.gene86156.t1 | MTR_8g011710 | 45.070 | 142 | 78 | 0 | 6 | 147 | 904 | 1045 | 2.65e-34 | 126 |
| MS.gene86156.t1 | MTR_4g020700 | 46.281 | 121 | 65 | 0 | 10 | 130 | 1407 | 1527 | 3.50e-34 | 126 |
| MS.gene86156.t1 | MTR_4g020700 | 46.281 | 121 | 65 | 0 | 10 | 130 | 1407 | 1527 | 3.65e-34 | 126 |
| MS.gene86156.t1 | MTR_5g036240 | 47.059 | 119 | 63 | 0 | 11 | 129 | 740 | 858 | 3.74e-34 | 126 |
| MS.gene86156.t1 | MTR_4g020850 | 48.800 | 125 | 64 | 0 | 6 | 130 | 1608 | 1732 | 3.99e-34 | 126 |
| MS.gene86156.t1 | MTR_4g021057 | 50.000 | 120 | 59 | 1 | 10 | 129 | 1428 | 1546 | 6.90e-34 | 125 |
| MS.gene86156.t1 | MTR_0286s0020 | 46.281 | 121 | 65 | 0 | 10 | 130 | 409 | 529 | 1.47e-33 | 124 |
| MS.gene86156.t1 | MTR_4g021023 | 47.500 | 120 | 63 | 0 | 10 | 129 | 1411 | 1530 | 2.70e-33 | 123 |
| MS.gene86156.t1 | MTR_4g021023 | 47.500 | 120 | 63 | 0 | 10 | 129 | 1160 | 1279 | 3.03e-33 | 123 |
| MS.gene86156.t1 | MTR_6g015450 | 48.387 | 124 | 32 | 1 | 4 | 127 | 15 | 106 | 6.42e-33 | 113 |
| MS.gene86156.t1 | MTR_8g011950 | 46.825 | 126 | 67 | 0 | 4 | 129 | 859 | 984 | 7.42e-33 | 122 |
| MS.gene86156.t1 | MTR_8g012200 | 46.774 | 124 | 66 | 0 | 4 | 127 | 887 | 1010 | 1.14e-32 | 122 |
| MS.gene86156.t1 | MTR_8g037235 | 46.154 | 130 | 69 | 1 | 1 | 129 | 793 | 922 | 1.81e-32 | 121 |
| MS.gene86156.t1 | MTR_8g020430 | 49.153 | 118 | 60 | 0 | 4 | 121 | 856 | 973 | 1.85e-32 | 121 |
| MS.gene86156.t1 | MTR_3g022930 | 48.387 | 124 | 64 | 0 | 4 | 127 | 888 | 1011 | 1.99e-32 | 121 |
| MS.gene86156.t1 | MTR_5g029940 | 40.541 | 148 | 79 | 2 | 1 | 148 | 5 | 143 | 2.16e-32 | 113 |
| MS.gene86156.t1 | MTR_5g047530 | 45.238 | 126 | 69 | 0 | 6 | 131 | 883 | 1008 | 2.80e-32 | 120 |
| MS.gene86156.t1 | MTR_8g012190 | 46.721 | 122 | 65 | 0 | 6 | 127 | 878 | 999 | 4.01e-32 | 120 |
| MS.gene86156.t1 | MTR_8g012190 | 46.721 | 122 | 65 | 0 | 6 | 127 | 872 | 993 | 4.50e-32 | 120 |
| MS.gene86156.t1 | MTR_7g025250 | 50.000 | 124 | 62 | 0 | 4 | 127 | 896 | 1019 | 1.67e-31 | 118 |
| MS.gene86156.t1 | MTR_4g020640 | 45.133 | 113 | 62 | 0 | 18 | 130 | 1431 | 1543 | 2.06e-31 | 118 |
| MS.gene86156.t1 | MTR_4g020640 | 45.133 | 113 | 62 | 0 | 18 | 130 | 1431 | 1543 | 2.11e-31 | 118 |
| MS.gene86156.t1 | MTR_5g047480 | 44.444 | 126 | 70 | 0 | 6 | 131 | 883 | 1008 | 2.57e-31 | 118 |
| MS.gene86156.t1 | MTR_2g437560 | 45.161 | 124 | 68 | 0 | 4 | 127 | 856 | 979 | 6.44e-31 | 117 |
| MS.gene86156.t1 | MTR_4g020490 | 44.915 | 118 | 65 | 0 | 10 | 127 | 1533 | 1650 | 1.07e-30 | 116 |
| MS.gene86156.t1 | MTR_4g020550 | 45.133 | 113 | 62 | 0 | 18 | 130 | 1371 | 1483 | 1.19e-30 | 116 |
| MS.gene86156.t1 | MTR_0468s0010 | 47.458 | 118 | 62 | 0 | 10 | 127 | 857 | 974 | 2.72e-30 | 115 |
| MS.gene86156.t1 | MTR_4g021170 | 44.262 | 122 | 68 | 0 | 6 | 127 | 1385 | 1506 | 1.42e-29 | 113 |
| MS.gene86156.t1 | MTR_8g011870 | 43.333 | 120 | 67 | 1 | 11 | 129 | 121 | 240 | 3.64e-29 | 109 |
| MS.gene86156.t1 | MTR_4g021030 | 46.847 | 111 | 56 | 1 | 36 | 143 | 1 | 111 | 9.85e-29 | 103 |
| MS.gene86156.t1 | MTR_0552s0040 | 42.609 | 115 | 66 | 0 | 11 | 125 | 337 | 451 | 2.31e-27 | 106 |
| MS.gene86156.t1 | MTR_4g021095 | 42.609 | 115 | 66 | 0 | 11 | 125 | 1277 | 1391 | 2.33e-27 | 106 |
| MS.gene86156.t1 | MTR_4g017740 | 48.624 | 109 | 53 | 1 | 36 | 141 | 1 | 109 | 3.20e-27 | 100 |
| MS.gene86156.t1 | MTR_5g071610 | 41.463 | 123 | 72 | 0 | 5 | 127 | 878 | 1000 | 2.68e-26 | 103 |
| MS.gene86156.t1 | MTR_4g014580 | 58.242 | 91 | 31 | 2 | 45 | 129 | 606 | 695 | 2.60e-25 | 100 |
| MS.gene86156.t1 | MTR_6g015575 | 42.636 | 129 | 27 | 2 | 1 | 129 | 24 | 105 | 1.18e-22 | 88.6 |
| MS.gene86156.t1 | MTR_6g015655 | 54.545 | 77 | 32 | 3 | 1 | 77 | 1 | 74 | 1.60e-21 | 83.6 |
| MS.gene86156.t1 | MTR_4g015050 | 68.421 | 57 | 17 | 1 | 4 | 60 | 822 | 877 | 2.43e-19 | 84.0 |
| MS.gene86156.t1 | MTR_4g020760 | 38.583 | 127 | 49 | 3 | 4 | 129 | 526 | 624 | 4.82e-19 | 82.8 |
| MS.gene86156.t1 | MTR_6g015720 | 55.000 | 60 | 23 | 2 | 7 | 66 | 773 | 828 | 9.17e-17 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGTGGTTGTTTGCTTCC+TGG | 0.340260 | 6.3:-9611846 | MS.gene86156:CDS |
| TTGAGAATGGTAACAATGTT+TGG | 0.353917 | 6.3:-9611458 | MS.gene86156:CDS |
| GAATTGAAGGTTAACCAATC+TGG | 0.364647 | 6.3:+9611813 | None:intergenic |
| AGAAGAACCTTCAGAATTGA+AGG | 0.423216 | 6.3:+9611800 | None:intergenic |
| ACATTTGCCCTTCCACTTGA+GGG | 0.449002 | 6.3:+9611763 | None:intergenic |
| TGTTGTCTATTCTTCAACTT+CGG | 0.457894 | 6.3:-9611721 | MS.gene86156:CDS |
| CTCTCTCTTGTATAGCTGAA+TGG | 0.462196 | 6.3:+9611641 | None:intergenic |
| TAACAGAATATGGATGAGAA+TGG | 0.463377 | 6.3:-9611870 | None:intergenic |
| GTTATGTTGTAAGTCATCCA+TGG | 0.475057 | 6.3:+9611421 | None:intergenic |
| CTGGTGACAGATATCCAGAT+TGG | 0.477169 | 6.3:-9611827 | MS.gene86156:CDS |
| TGATGGCCTTAGAAACTTGT+TGG | 0.484226 | 6.3:-9611685 | MS.gene86156:CDS |
| AACATTTGCCCTTCCACTTG+AGG | 0.497432 | 6.3:+9611762 | None:intergenic |
| AACAGAATATGGATGAGAAT+GGG | 0.508394 | 6.3:-9611869 | None:intergenic |
| CTGCCCCTCCTCATCTTCAA+AGG | 0.516785 | 6.3:+9611608 | None:intergenic |
| TGGTTAACCTTCAATTCTGA+AGG | 0.522559 | 6.3:-9611807 | MS.gene86156:CDS |
| TCGGATAATATAACATCTGA+TGG | 0.527938 | 6.3:-9611702 | MS.gene86156:CDS |
| TTTGAAGTCCCTCAAGTGGA+AGG | 0.537133 | 6.3:-9611771 | MS.gene86156:CDS |
| ACAACTTCCACTTTGTTACC+AGG | 0.539602 | 6.3:+9611564 | None:intergenic |
| TCATCCATGGGCGTCGCTTG+AGG | 0.553496 | 6.3:+9611434 | None:intergenic |
| CAATCTGGATATCTGTCACC+AGG | 0.564533 | 6.3:+9611828 | None:intergenic |
| AGTAGCCGCCTTTGAAGATG+AGG | 0.567239 | 6.3:-9611616 | MS.gene86156:CDS |
| TGGTCCTCAAGCGACGCCCA+TGG | 0.578818 | 6.3:-9611438 | MS.gene86156:CDS |
| TTATGTTGTAAGTCATCCAT+GGG | 0.590193 | 6.3:+9611422 | None:intergenic |
| AGCCGCCTTTGAAGATGAGG+AGG | 0.598219 | 6.3:-9611613 | MS.gene86156:CDS |
| TTGAAGTCCCTCAAGTGGAA+GGG | 0.598230 | 6.3:-9611770 | MS.gene86156:CDS |
| TATAGAACCTGGTAACAAAG+TGG | 0.620153 | 6.3:-9611571 | MS.gene86156:CDS |
| CATTCAGCTATACAAGAGAG+AGG | 0.647715 | 6.3:-9611640 | MS.gene86156:CDS |
| ATGGATGAGAATGGGAGTGG+TGG | 0.648413 | 6.3:-9611861 | MS.gene86156:CDS |
| GCCGCCTTTGAAGATGAGGA+GGG | 0.652253 | 6.3:-9611612 | MS.gene86156:CDS |
| CCCCTCCTCATCTTCAAAGG+CGG | 0.658665 | 6.3:+9611611 | None:intergenic |
| AATATGGATGAGAATGGGAG+TGG | 0.660717 | 6.3:-9611864 | MS.gene86156:CDS |
| GTAGTATCAAGTATAGAACC+TGG | 0.669829 | 6.3:-9611582 | MS.gene86156:CDS |
| CCGCCTTTGAAGATGAGGAG+GGG | 0.701357 | 6.3:-9611611 | MS.gene86156:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTCATATATGATGAAAAAG+TGG | - | chr6.3:9611799-9611818 | MS.gene86156:CDS | 25.0% |
| TCGGATAATATAACATCTGA+TGG | - | chr6.3:9611593-9611612 | MS.gene86156:CDS | 30.0% | |
| TGTTGTCTATTCTTCAACTT+CGG | - | chr6.3:9611574-9611593 | MS.gene86156:CDS | 30.0% | |
| TTGAGAATGGTAACAATGTT+TGG | - | chr6.3:9611837-9611856 | MS.gene86156:CDS | 30.0% | |
| ! | GTTGGTGAAAAATTACACAA+AGG | - | chr6.3:9611628-9611647 | MS.gene86156:CDS | 30.0% |
| ! | TTTTCACCAACAAGTTTCTA+AGG | + | chr6.3:9611619-9611638 | None:intergenic | 30.0% |
| AGAAGAACCTTCAGAATTGA+AGG | + | chr6.3:9611498-9611517 | None:intergenic | 35.0% | |
| GAATTGAAGGTTAACCAATC+TGG | + | chr6.3:9611485-9611504 | None:intergenic | 35.0% | |
| GTAGTATCAAGTATAGAACC+TGG | - | chr6.3:9611713-9611732 | MS.gene86156:CDS | 35.0% | |
| TATAGAACCTGGTAACAAAG+TGG | - | chr6.3:9611724-9611743 | MS.gene86156:CDS | 35.0% | |
| TGGTTAACCTTCAATTCTGA+AGG | - | chr6.3:9611488-9611507 | MS.gene86156:CDS | 35.0% | |
| !!! | GTTGTCGTTGTTTTTGAGAA+TGG | - | chr6.3:9611749-9611768 | MS.gene86156:CDS | 35.0% |
| ACAACTTCCACTTTGTTACC+AGG | + | chr6.3:9611734-9611753 | None:intergenic | 40.0% | |
| CATTCAGCTATACAAGAGAG+AGG | - | chr6.3:9611655-9611674 | MS.gene86156:CDS | 40.0% | |
| CTCTCTCTTGTATAGCTGAA+TGG | + | chr6.3:9611657-9611676 | None:intergenic | 40.0% | |
| ! | TGATGGCCTTAGAAACTTGT+TGG | - | chr6.3:9611610-9611629 | MS.gene86156:CDS | 40.0% |
| !!! | TACTTTTGAAGTCCCTCAAG+TGG | - | chr6.3:9611520-9611539 | MS.gene86156:CDS | 40.0% |
| AACATTTGCCCTTCCACTTG+AGG | + | chr6.3:9611536-9611555 | None:intergenic | 45.0% | |
| ACATTTGCCCTTCCACTTGA+GGG | + | chr6.3:9611535-9611554 | None:intergenic | 45.0% | |
| CAATCTGGATATCTGTCACC+AGG | + | chr6.3:9611470-9611489 | None:intergenic | 45.0% | |
| CTGGTGACAGATATCCAGAT+TGG | - | chr6.3:9611468-9611487 | MS.gene86156:CDS | 45.0% | |
| TTGAAGTCCCTCAAGTGGAA+GGG | - | chr6.3:9611525-9611544 | MS.gene86156:CDS | 45.0% | |
| ! | TTTGAAGTCCCTCAAGTGGA+AGG | - | chr6.3:9611524-9611543 | MS.gene86156:CDS | 45.0% |
| AGTGGTGGTTGTTTGCTTCC+TGG | - | chr6.3:9611449-9611468 | MS.gene86156:CDS | 50.0% | |
| ATGGATGAGAATGGGAGTGG+TGG | - | chr6.3:9611434-9611453 | MS.gene86156:CDS | 50.0% | |
| ! | AGTAGCCGCCTTTGAAGATG+AGG | - | chr6.3:9611679-9611698 | MS.gene86156:CDS | 50.0% |
| CCCCTCCTCATCTTCAAAGG+CGG | + | chr6.3:9611687-9611706 | None:intergenic | 55.0% | |
| CTGCCCCTCCTCATCTTCAA+AGG | + | chr6.3:9611690-9611709 | None:intergenic | 55.0% | |
| ! | AGCCGCCTTTGAAGATGAGG+AGG | - | chr6.3:9611682-9611701 | MS.gene86156:CDS | 55.0% |
| ! | CCGCCTTTGAAGATGAGGAG+GGG | - | chr6.3:9611684-9611703 | MS.gene86156:CDS | 55.0% |
| ! | GCCGCCTTTGAAGATGAGGA+GGG | - | chr6.3:9611683-9611702 | MS.gene86156:CDS | 55.0% |
| ! | TCATCCATGGGCGTCGCTTG+AGG | + | chr6.3:9611864-9611883 | None:intergenic | 60.0% |
| TGGTCCTCAAGCGACGCCCA+TGG | - | chr6.3:9611857-9611876 | MS.gene86156:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 9611434 | 9611883 | 9611434 | ID=MS.gene86156 |
| chr6.3 | mRNA | 9611434 | 9611883 | 9611434 | ID=MS.gene86156.t1;Parent=MS.gene86156 |
| chr6.3 | exon | 9611434 | 9611883 | 9611434 | ID=MS.gene86156.t1.exon1;Parent=MS.gene86156.t1 |
| chr6.3 | CDS | 9611434 | 9611883 | 9611434 | ID=cds.MS.gene86156.t1;Parent=MS.gene86156.t1 |
| Gene Sequence |
| Protein sequence |
