AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86230.t1 MTR_5g032780 98.899 636 7 0 1 636 1 636 0.0 1298
MS.gene86230.t1 MTR_8g069330 71.743 637 138 9 1 636 1 596 0.0 866
MS.gene86230.t1 MTR_4g134210 48.000 525 227 7 1 524 1 480 7.52e-162 477
MS.gene86230.t1 MTR_5g015790 46.250 560 251 9 1 551 1 519 3.72e-159 471
MS.gene86230.t1 MTR_1g014070 47.036 523 236 10 1 518 1 487 1.78e-152 454
MS.gene86230.t1 MTR_1g106430 42.804 542 270 7 1 537 1 507 1.63e-146 439
MS.gene86230.t1 MTR_2g027410 40.758 633 284 12 1 610 1 565 3.37e-146 436
MS.gene86230.t1 MTR_4g093850 39.852 542 276 8 5 519 23 541 2.04e-126 388
MS.gene86230.t1 MTR_2g009340 39.229 571 288 10 5 539 23 570 1.04e-125 387
MS.gene86230.t1 MTR_2g027410 39.535 559 249 11 75 610 1 493 4.33e-119 363
MS.gene86230.t1 MTR_2g018750 40.038 532 280 9 1 523 1 502 1.59e-113 353
MS.gene86230.t1 MTR_1g064360 38.241 523 283 7 1 523 1 483 3.09e-111 345
MS.gene86230.t1 MTR_1g094850 39.171 531 274 11 1 519 1 494 1.38e-110 345
MS.gene86230.t1 MTR_5g016060 37.244 537 241 10 22 529 36 505 2.68e-108 340
MS.gene86230.t1 MTR_8g090220 35.962 520 259 7 16 515 42 507 7.01e-108 335
MS.gene86230.t1 MTR_8g090220 35.145 552 273 8 16 536 42 539 1.08e-107 339
MS.gene86230.t1 MTR_8g078900 38.031 518 266 9 28 517 22 512 1.26e-105 334
MS.gene86230.t1 MTR_2g084595 37.917 509 282 8 24 524 21 503 2.14e-104 330
MS.gene86230.t1 MTR_8g078900 38.264 507 258 9 39 517 3 482 4.12e-103 327
MS.gene86230.t1 MTR_7g073980 38.189 508 269 10 22 524 29 496 1.11e-101 323
MS.gene86230.t1 MTR_8g078900 39.738 458 239 7 70 517 42 472 5.00e-101 321
MS.gene86230.t1 MTR_4g022330 36.106 565 294 10 22 581 30 532 1.44e-97 311
MS.gene86230.t1 MTR_4g093850 35.845 491 265 7 5 468 23 490 5.96e-95 302
MS.gene86230.t1 MTR_1g088885 37.196 535 275 14 12 530 18 507 2.00e-93 301
MS.gene86230.t1 MTR_4g095430 36.217 497 276 7 23 519 10 465 4.08e-93 299
MS.gene86230.t1 MTR_7g108290 35.884 588 314 14 12 584 18 557 1.56e-90 295
MS.gene86230.t1 MTR_2g048390 37.006 481 261 9 1 468 1 452 3.35e-89 285
MS.gene86230.t1 MTR_3g062540 34.269 499 271 7 21 519 19 460 6.41e-86 280
MS.gene86230.t1 MTR_2g084595 35.619 452 259 8 24 469 21 446 1.11e-80 262
MS.gene86230.t1 MTR_5g016060 35.463 454 199 9 105 529 3 391 1.15e-80 264
MS.gene86230.t1 MTR_8g078900 33.966 474 258 8 23 468 17 463 1.73e-79 259
MS.gene86230.t1 MTR_3g062540 33.755 474 257 7 46 519 2 418 1.29e-77 256
MS.gene86230.t1 MTR_5g075640 33.142 522 295 9 22 539 21 492 1.21e-76 255
MS.gene86230.t1 MTR_8g078900 35.697 409 226 6 70 468 42 423 1.06e-74 246
MS.gene86230.t1 MTR_1g082690 31.225 506 267 11 27 525 59 490 5.94e-72 242
MS.gene86230.t1 MTR_5g075640 32.530 498 282 8 46 539 2 449 1.57e-69 235
MS.gene86230.t1 MTR_4g011530 38.511 309 149 4 211 519 46 313 2.69e-66 224
MS.gene86230.t1 MTR_2g102730 38.835 309 150 4 211 519 24 293 3.02e-62 213
MS.gene86230.t1 MTR_4g045767 29.075 454 251 13 74 519 52 442 8.89e-47 173
MS.gene86230.t1 MTR_4g074190 27.481 524 296 13 27 523 4 470 9.93e-46 171
MS.gene86230.t1 MTR_4g074190 27.766 479 264 11 70 523 3 424 1.06e-43 164
MS.gene86230.t1 MTR_1g035720 25.832 511 281 12 64 541 36 481 3.44e-41 157
MS.gene86230.t1 MTR_4g074190 26.539 471 262 13 27 470 4 417 5.95e-36 140
MS.gene86230.t1 MTR_1g105870 32.161 199 119 4 33 230 38 221 5.77e-21 93.6
MS.gene86230.t1 MTR_2g010890 24.821 419 247 10 94 505 1 358 1.12e-20 95.9
MS.gene86230.t1 MTR_4g080720 64.912 57 20 0 1 57 1 57 1.73e-19 83.2
MS.gene86230.t1 MTR_2g048375 32.680 153 85 5 485 634 1 138 8.39e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86230.t1 AT1G67900 73.023 645 150 11 1 636 1 630 0.0 864
MS.gene86230.t1 AT1G67900 73.023 645 150 11 1 636 1 630 0.0 864
MS.gene86230.t1 AT1G67900 73.023 645 150 11 1 636 1 630 0.0 864
MS.gene86230.t1 AT1G67900 73.023 645 150 11 1 636 1 630 0.0 864
MS.gene86230.t1 AT1G67900 73.023 645 150 11 1 636 1 630 0.0 864
MS.gene86230.t1 AT1G67900 73.023 645 150 11 1 636 1 630 0.0 864
MS.gene86230.t1 AT3G26490 49.085 656 244 15 1 635 1 587 0.0 561
MS.gene86230.t1 AT3G26490 49.085 656 244 15 1 635 1 587 0.0 561
MS.gene86230.t1 AT3G26490 49.085 656 244 15 1 635 1 587 0.0 561
MS.gene86230.t1 AT3G26490 53.061 490 176 13 1 469 1 457 3.97e-161 472
MS.gene86230.t1 AT2G14820 45.149 536 247 8 1 528 1 497 3.06e-152 454
MS.gene86230.t1 AT2G14820 45.149 536 247 8 1 528 1 497 3.06e-152 454
MS.gene86230.t1 AT5G47800 42.561 531 262 6 1 531 1 488 1.42e-146 436
MS.gene86230.t1 AT5G47800 42.561 531 262 6 1 531 1 488 1.42e-146 436
MS.gene86230.t1 AT5G47800 42.561 531 262 6 1 531 1 488 1.42e-146 436
MS.gene86230.t1 AT5G47800 42.561 531 262 6 1 531 114 601 7.50e-145 436
MS.gene86230.t1 AT5G67440 43.595 523 246 8 1 520 1 477 1.87e-142 427
MS.gene86230.t1 AT5G67440 43.595 523 246 8 1 520 1 477 1.87e-142 427
MS.gene86230.t1 AT4G37590 40.336 595 263 12 1 582 1 516 6.65e-141 422
MS.gene86230.t1 AT4G31820 42.533 529 241 10 1 523 1 472 1.64e-132 401
MS.gene86230.t1 AT5G67440 43.568 482 224 7 41 520 5 440 1.49e-127 387
MS.gene86230.t1 AT2G23050 41.210 529 230 11 1 523 1 454 8.86e-127 383
MS.gene86230.t1 AT5G64330 37.651 579 289 8 7 519 33 605 1.21e-121 376
MS.gene86230.t1 AT5G64330 35.913 646 332 11 7 584 33 664 4.00e-121 377
MS.gene86230.t1 AT5G64330 37.306 579 289 9 39 549 6 578 4.02e-116 360
MS.gene86230.t1 AT4G31820 41.807 476 215 9 53 523 1 419 5.34e-116 356
MS.gene86230.t1 AT1G30440 39.429 525 291 9 1 517 1 506 5.58e-114 356
MS.gene86230.t1 AT5G03250 37.594 532 297 6 1 523 1 506 6.78e-113 351
MS.gene86230.t1 AT5G13600 40.377 530 276 10 1 523 1 497 7.81e-111 345
MS.gene86230.t1 AT5G67385 38.976 508 258 8 15 519 16 474 9.46e-106 333
MS.gene86230.t1 AT5G48800 38.856 507 250 9 22 523 37 488 2.45e-98 313
MS.gene86230.t1 AT5G67385 38.025 476 245 7 47 519 1 429 1.23e-96 307
MS.gene86230.t1 AT5G67385 38.025 476 245 7 47 519 1 429 1.23e-96 307
MS.gene86230.t1 AT5G67385 38.025 476 245 7 47 519 1 429 1.23e-96 307
MS.gene86230.t1 AT5G67385 38.025 476 245 7 47 519 1 429 1.23e-96 307
MS.gene86230.t1 AT5G10250 34.549 521 258 8 22 519 46 506 9.03e-93 299
MS.gene86230.t1 AT3G08570 37.255 510 276 11 13 519 22 490 5.98e-92 297
MS.gene86230.t1 AT5G66560 35.846 544 259 10 28 517 21 528 2.39e-91 297
MS.gene86230.t1 AT1G03010 37.972 503 272 12 24 519 7 476 1.00e-90 293
MS.gene86230.t1 AT3G44820 33.447 589 332 15 26 591 23 574 1.21e-90 295
MS.gene86230.t1 AT1G03010 37.972 503 272 12 24 519 34 503 2.03e-90 293
MS.gene86230.t1 AT1G03010 37.597 516 280 13 11 519 22 502 2.11e-90 293
MS.gene86230.t1 AT5G66560 35.835 533 252 10 39 517 3 499 1.10e-89 291
MS.gene86230.t1 AT5G10250 34.638 511 256 9 22 519 46 491 1.60e-89 290
MS.gene86230.t1 AT5G66560 38.478 460 235 9 70 517 66 489 1.84e-89 291
MS.gene86230.t1 AT2G23050 39.952 413 171 8 113 523 1 338 1.18e-86 275
MS.gene86230.t1 AT3G49970 36.344 465 216 11 39 502 7 392 2.86e-86 279
MS.gene86230.t1 AT3G49970 36.344 465 216 11 39 502 7 392 2.86e-86 279
MS.gene86230.t1 AT3G08660 34.808 520 257 12 8 519 21 466 2.03e-83 273
MS.gene86230.t1 AT2G47860 35.341 498 282 10 24 519 35 494 2.08e-81 270
MS.gene86230.t1 AT3G50840 34.921 504 276 8 28 529 18 471 1.74e-78 260
MS.gene86230.t1 AT2G30520 32.265 499 284 9 21 519 25 469 3.93e-78 260
MS.gene86230.t1 AT2G47860 33.460 526 277 11 24 519 35 517 2.73e-76 256
MS.gene86230.t1 AT5G03250 36.190 420 233 6 113 523 1 394 4.91e-76 251
MS.gene86230.t1 AT3G08660 34.858 459 225 10 69 519 14 406 1.41e-73 246
MS.gene86230.t1 AT2G30520 32.889 450 250 8 70 519 24 421 8.43e-72 241
MS.gene86230.t1 AT2G30520 32.889 450 250 8 70 519 24 421 8.43e-72 241
MS.gene86230.t1 AT3G50840 33.874 493 258 9 47 529 1 435 1.05e-71 241
MS.gene86230.t1 AT3G50840 33.874 493 258 9 47 529 1 435 1.05e-71 241
MS.gene86230.t1 AT5G66560 32.465 499 241 10 28 469 21 480 6.59e-65 221
MS.gene86230.t1 AT1G52770 39.677 310 152 4 211 519 28 303 2.81e-64 219
MS.gene86230.t1 AT3G08660 31.557 469 243 12 8 470 21 417 8.09e-61 209
MS.gene86230.t1 AT3G15570 37.705 305 151 4 220 519 38 308 2.94e-59 206
MS.gene86230.t1 AT2G47860 32.662 447 261 10 24 468 35 443 4.90e-58 202
MS.gene86230.t1 AT2G47860 33.092 414 232 9 113 519 1 376 8.38e-55 195
MS.gene86230.t1 AT2G47860 33.092 414 232 9 113 519 1 376 8.38e-55 195
MS.gene86230.t1 AT2G47860 30.998 471 262 11 24 469 35 467 5.28e-53 189
MS.gene86230.t1 AT3G19850 26.483 472 265 8 69 517 51 463 1.65e-48 178
MS.gene86230.t1 AT5G48130 26.049 453 269 9 70 517 76 467 2.34e-42 162
MS.gene86230.t1 AT3G03510 30.000 450 207 12 82 519 8 361 8.58e-42 157
MS.gene86230.t1 AT1G50280 24.353 464 277 8 70 513 18 427 2.96e-39 151
MS.gene86230.t1 AT1G50280 24.353 464 277 8 70 513 51 460 4.55e-39 151
MS.gene86230.t1 AT5G17580 27.853 517 303 11 8 517 2 455 7.40e-39 151
MS.gene86230.t1 AT5G17580 28.032 503 292 11 22 517 1 440 1.98e-38 150
MS.gene86230.t1 AT3G22104 25.926 486 276 15 28 502 6 418 8.39e-32 130
MS.gene86230.t1 AT3G22104 24.638 414 231 11 98 502 2 343 1.09e-22 101
MS.gene86230.t1 AT3G22104 24.638 414 231 11 98 502 2 343 1.09e-22 101
MS.gene86230.t1 AT3G22104 24.638 414 231 11 98 502 2 343 1.09e-22 101

Find 112 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTAGCATAGACCTTTAT+TGG 0.142674 5.1:+23336139 MS.gene86230:CDS
CTTCTTTCTATTTCCGTTCT+TGG 0.164934 5.1:-23336259 None:intergenic
CTTTCACTCTTGTTCAGTTC+TGG 0.171118 5.1:-23337985 None:intergenic
GAGGATAATTGGAGTGTTTC+AGG 0.173097 5.1:+23338258 MS.gene86230:CDS
TTTCCGTTCTTGGAAATATT+TGG 0.180699 5.1:-23336249 None:intergenic
CATGATGAATTTGATGAAAA+TGG 0.208213 5.1:+23338336 MS.gene86230:CDS
AATCAGGAAGTTGAACTATT+TGG 0.235212 5.1:-23335690 None:intergenic
ACTTGAACAACAACTCTTAA+TGG 0.251309 5.1:-23338087 None:intergenic
TCCAAGTTTCATAAACTTCA+TGG 0.285144 5.1:-23335076 None:intergenic
AGAGTTGAAGACTTTGATTT+TGG 0.291628 5.1:-23338291 None:intergenic
TTTCAACTTCTTCAGTCATT+TGG 0.299710 5.1:-23335822 None:intergenic
TGATCATATCTTGCAAATTC+TGG 0.304119 5.1:-23336834 None:intergenic
GTATTCAAGGTGAGGATAAT+TGG 0.310231 5.1:+23338247 MS.gene86230:CDS
AGCTTGCTTCCAACAGAAAA+AGG 0.312871 5.1:+23336369 MS.gene86230:CDS
TGAAGAAGCATTGAGAATAT+TGG 0.313880 5.1:-23336433 None:intergenic
TTCAAAGCATAGGCTTCTAT+TGG 0.325252 5.1:+23336335 MS.gene86230:CDS
ATGACTGAAGAAGTTGAAAA+AGG 0.332313 5.1:+23335826 MS.gene86230:CDS
CTATCATTGTTCTCCAATAA+AGG 0.335482 5.1:-23336152 None:intergenic
TGAAAATTTATGCATCTAGA+TGG 0.337434 5.1:+23336223 MS.gene86230:CDS
TCTACACTTCGGAAGCTATA+AGG 0.341634 5.1:+23335115 MS.gene86230:CDS
GCCATGAAGTTTATGAAACT+TGG 0.345527 5.1:+23335075 None:intergenic
TCCGAAGTGTAGAAAGTATC+TGG 0.347377 5.1:-23335105 None:intergenic
CCACAAAGGCGTTGCATTTG+TGG 0.361175 5.1:+23335929 MS.gene86230:CDS
TCCACTCCACCCGGAAAATC+AGG 0.369267 5.1:-23335706 None:intergenic
TGTGGTCTGAAGACTTACTA+TGG 0.375499 5.1:-23335911 None:intergenic
TGTGACAAACTCACCTTTGT+AGG 0.375606 5.1:-23336015 None:intergenic
GTGTCGCCAATGAGATTTGC+AGG 0.381277 5.1:-23336198 None:intergenic
TGAAAGAAGGCGGTCACGTT+CGG 0.382002 5.1:+23336650 MS.gene86230:CDS
CAAGCTAGAGCAGCAGCATC+TGG 0.386191 5.1:+23338123 MS.gene86230:CDS
TCTTTGCAGGCATAAGCATT+TGG 0.386345 5.1:-23335632 None:intergenic
TTGGTAAATGATGATGGAAT+TGG 0.390964 5.1:+23338360 MS.gene86230:CDS
ATTCTGAGCATAATCTTTGC+AGG 0.392741 5.1:-23335645 None:intergenic
CCACAAATGCAACGCCTTTG+TGG 0.394661 5.1:-23335929 None:intergenic
CTTGAACAACAACTCTTAAT+GGG 0.398186 5.1:-23338086 None:intergenic
CATCTCTTGCAACTTCTTGA+AGG 0.405047 5.1:-23336767 None:intergenic
TGAGTTGGTGATGACTATAT+TGG 0.420160 5.1:+23336566 MS.gene86230:CDS
GTGATCTCATAATTCAAGTA+AGG 0.425248 5.1:+23335264 MS.gene86230:CDS
TGCTTCTTCATCTTCAAAGA+TGG 0.426814 5.1:+23336446 MS.gene86230:CDS
TGTCACATAGTCATTCTAGA+AGG 0.445951 5.1:+23336031 MS.gene86230:CDS
AGCTCTACTACAAGTATTCA+AGG 0.447165 5.1:+23338234 MS.gene86230:CDS
GGCATAAGCATTTGGATAGC+AGG 0.447706 5.1:-23335624 None:intergenic
TTGTGTAGGAAGAGCACAAA+TGG 0.459576 5.1:-23338403 None:intergenic
TGAATTTGATGAAAATGGTT+TGG 0.459746 5.1:+23338341 MS.gene86230:CDS
TACCTTGAGATAAATGTCAA+TGG 0.464242 5.1:-23336874 None:intergenic
ACAATGATAGCAATCAAATC+TGG 0.464906 5.1:+23336165 MS.gene86230:CDS
AACGATCTCGGAGGGCTAGT+TGG 0.466201 5.1:-23336618 None:intergenic
TTCAATTCTTGCATCCTCCA+TGG 0.467888 5.1:+23335877 MS.gene86230:CDS
TCAAAAGTCTTCAACGATCT+CGG 0.468557 5.1:-23336630 None:intergenic
GCATTTGTGGTCTGAAGACT+TGG 0.469383 5.1:+23335942 MS.gene86230:CDS
ACTTTGAATTACAAGAAAGT+AGG 0.470597 5.1:+23336685 MS.gene86230:CDS
ATTTGGTGATGAGATGAATC+AGG 0.473504 5.1:-23335673 None:intergenic
ATGCTTTGAAGTTATTTCGC+TGG 0.473982 5.1:-23336322 None:intergenic
GAAAGTTTGAGAATCAAATC+AGG 0.487700 5.1:+23336084 MS.gene86230:CDS
AAGAAGGGTAGGACTTCAAT+TGG 0.492020 5.1:+23336476 MS.gene86230:CDS
AAATGTCAATGGCTCTATAG+AGG 0.499230 5.1:-23336863 None:intergenic
AGAATCAAATCAGGAAGTAA+AGG 0.499839 5.1:+23336093 MS.gene86230:CDS
AAGGTTCCTGCAAATCTCAT+TGG 0.503101 5.1:+23336192 MS.gene86230:CDS
TTGGAACAGTTCATGTTACA+AGG 0.510924 5.1:+23336585 MS.gene86230:CDS
AATGGTTTGGTAAATGATGA+TGG 0.512979 5.1:+23338354 MS.gene86230:CDS
ATTCTTGCATCCTCCATGGT+TGG 0.519380 5.1:+23335881 MS.gene86230:CDS
GCTAGAGCAGCAGCATCTGG+TGG 0.522418 5.1:+23338126 MS.gene86230:CDS
TTGAATTACAAGAAAGTAGG+AGG 0.526848 5.1:+23336688 MS.gene86230:CDS
ATTGAAAGTGGCAAAACTTG+TGG 0.529222 5.1:+23336737 MS.gene86230:CDS
GCGAAATAACTTCAAAGCAT+AGG 0.531559 5.1:+23336325 MS.gene86230:CDS
CTTGCAGCTACAATGTTGTA+AGG 0.538418 5.1:-23335781 None:intergenic
GCATAAGCATTTGGATAGCA+GGG 0.539705 5.1:-23335623 None:intergenic
AATAGTTTCAATGCATCTGC+TGG 0.540399 5.1:-23335971 None:intergenic
TTGCATCCTCCATGGTTGGA+AGG 0.541728 5.1:+23335885 MS.gene86230:CDS
ACATAGTTCAAAATTGAAAG+TGG 0.541920 5.1:+23336725 MS.gene86230:CDS
TCCAGATACTTTCTACACTT+CGG 0.550104 5.1:+23335104 MS.gene86230:CDS
AGCCATTGACATTTATCTCA+AGG 0.550508 5.1:+23336872 MS.gene86230:CDS
TGATCTCATAATTCAAGTAA+GGG 0.550839 5.1:+23335265 MS.gene86230:CDS
GTGACAAACTCACCTTTGTA+GGG 0.551753 5.1:-23336014 None:intergenic
TTGTGTGCTAAGTTCTGCTA+TGG 0.551870 5.1:+23335739 MS.gene86230:CDS
CAAAGATGGAATTAGCAAGA+AGG 0.552149 5.1:+23336460 MS.gene86230:CDS
AAACTTGATTCACAAGCTTG+AGG 0.555629 5.1:+23335849 MS.gene86230:CDS
AAAGATGGAATTAGCAAGAA+GGG 0.557589 5.1:+23336461 MS.gene86230:CDS
TTACCAAATATTTCCAAGAA+CGG 0.560310 5.1:+23336246 MS.gene86230:CDS
CAGAACTGAACAAGAGTGAA+AGG 0.560948 5.1:+23337986 MS.gene86230:CDS
GATCTCGGAGGGCTAGTTGG+TGG 0.563074 5.1:-23336615 None:intergenic
TGAACTATGTGATGCTGAAG+AGG 0.563744 5.1:-23336712 None:intergenic
ATAGTCATTCTAGAAGGATG+AGG 0.566210 5.1:+23336037 MS.gene86230:CDS
TCAAATGCTTCCACTCCACC+CGG 0.574131 5.1:-23335715 None:intergenic
AAGTGGCAAAACTTGTGGAT+AGG 0.577058 5.1:+23336742 MS.gene86230:CDS
ATCAGGAAGTAAAGGATGGT+GGG 0.580568 5.1:+23336101 MS.gene86230:CDS
ATGGAATTAGCAAGAAGGGT+AGG 0.583335 5.1:+23336465 MS.gene86230:CDS
CTATGGTATCCTTCCAACCA+TGG 0.584707 5.1:-23335894 None:intergenic
AGCAATCAAATCTGGTGGCA+AGG 0.587341 5.1:+23336173 MS.gene86230:CDS
GTCTGAGTCTAACTCAGACT+CGG 0.598165 5.1:+23336299 MS.gene86230:CDS
AATCAGGAAGTAAAGGATGG+TGG 0.603373 5.1:+23336100 MS.gene86230:CDS
TACTACAAGTATTCAAGGTG+AGG 0.607107 5.1:+23338239 MS.gene86230:CDS
AAGGGTAGGACTTCAATTGG+AGG 0.609288 5.1:+23336479 MS.gene86230:CDS
AGTCTTGAGCCACCCTACAA+AGG 0.609787 5.1:+23336002 MS.gene86230:CDS
TAGTCATTCTAGAAGGATGA+GGG 0.613006 5.1:+23336038 MS.gene86230:CDS
TCAAATCAGGAAGTAAAGGA+TGG 0.616909 5.1:+23336097 MS.gene86230:CDS
CATTTGTGGTCTGAAGACTT+GGG 0.618302 5.1:+23335943 MS.gene86230:CDS
TACAGTCTATGATGTTGAGT+TGG 0.618402 5.1:+23336551 MS.gene86230:CDS
AAGTCTTCAACGATCTCGGA+GGG 0.622487 5.1:-23336626 None:intergenic
ATGATAGCAATCAAATCTGG+TGG 0.634663 5.1:+23336168 MS.gene86230:CDS
GATCTCATAATTCAAGTAAG+GGG 0.642036 5.1:+23335266 MS.gene86230:CDS
GTCTGAGTTAGACTCAGACT+CGG 0.643618 5.1:-23336295 None:intergenic
TCTAAGAATGAAGCTTGCTG+AGG 0.644686 5.1:+23338311 MS.gene86230:CDS
AAAGCATTGCTTACAACACA+TGG 0.648620 5.1:+23338177 MS.gene86230:CDS
CATAAGCATTTGGATAGCAG+GGG 0.668690 5.1:-23335622 None:intergenic
AAAGTCTTCAACGATCTCGG+AGG 0.669614 5.1:-23336627 None:intergenic
ACAAACTCACCTTTGTAGGG+TGG 0.674969 5.1:-23336011 None:intergenic
TCATTTGTATAAGAAAGTGA+AGG 0.677583 5.1:-23336528 None:intergenic
AAGTAGATATCTTCTTCACA+AGG 0.678976 5.1:+23335289 MS.gene86230:CDS
TGGTATCCTTCCAACCATGG+AGG 0.717381 5.1:-23335891 None:intergenic
TGGTGATGAGATGAATCAGG+AGG 0.725233 5.1:-23335670 None:intergenic
AGTAAGTCTTCAGACCACAA+AGG 0.726529 5.1:+23335915 MS.gene86230:CDS
TATTAATAGATCATTCACTG+TGG 0.782652 5.1:-23336505 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTATATACTTATGA+AGG - chr5.1:23335558-23335577 None:intergenic 10.0%
!!! AAATACAATAAACATTTTAT+AGG - chr5.1:23336909-23336928 None:intergenic 10.0%
!!! AAGTATATAATTTTTTACTT+TGG + chr5.1:23335562-23335581 MS.gene86230:intron 10.0%
!!! AGGTTTTTTTATTTTTATTT+TGG + chr5.1:23337552-23337571 MS.gene86230:intron 10.0%
!!! TAATTTAAGTTTTTTTTTCA+AGG + chr5.1:23337595-23337614 MS.gene86230:intron 10.0%
!!! AATTTACTAAGCATTTTTTT+AGG - chr5.1:23338429-23338448 None:intergenic 15.0%
!!! ATTTTTATCAAGGTATAAAA+TGG - chr5.1:23336945-23336964 None:intergenic 15.0%
!!! TTGAATAAGACAATATTTTA+AGG - chr5.1:23337220-23337239 None:intergenic 15.0%
!! AAAAAATGCTTAGTAAATTG+TGG + chr5.1:23338430-23338449 MS.gene86230:CDS 20.0%
!! AAACATGTAAAGAAAAAAAG+TGG + chr5.1:23337121-23337140 MS.gene86230:intron 20.0%
!! AAATAGAGTGAATTCATAAA+TGG - chr5.1:23337477-23337496 None:intergenic 20.0%
!! AGATAATTTCATAACTCATT+TGG + chr5.1:23337331-23337350 MS.gene86230:intron 20.0%
!! ATATAGGACTAATAAAAACT+AGG + chr5.1:23337692-23337711 MS.gene86230:intron 20.0%
!! CAAAATTAAACAATCCAAAA+AGG - chr5.1:23335191-23335210 None:intergenic 20.0%
!! TGATAAAAATATATCTCAGA+TGG + chr5.1:23336955-23336974 MS.gene86230:intron 20.0%
!!! ATACTTCACTTTCAAATTTT+AGG + chr5.1:23337157-23337176 MS.gene86230:intron 20.0%
!!! TCTGAGATATATTTTTATCA+AGG - chr5.1:23336955-23336974 None:intergenic 20.0%
!!! TTGTCTTATTCAACATTTAT+TGG + chr5.1:23337227-23337246 MS.gene86230:intron 20.0%
! AAAATAACGAGCTTGAAATA+TGG - chr5.1:23337836-23337855 None:intergenic 25.0%
! ACATAGTTCAAAATTGAAAG+TGG + chr5.1:23336725-23336744 MS.gene86230:CDS 25.0%
! ACTAATATGACACATTGATA+CGG - chr5.1:23335487-23335506 None:intergenic 25.0%
! ACTTTGAATTACAAGAAAGT+AGG + chr5.1:23336685-23336704 MS.gene86230:CDS 25.0%
! AGTCCTATATTATTTGAACA+TGG - chr5.1:23337682-23337701 None:intergenic 25.0%
! CATGATGAATTTGATGAAAA+TGG + chr5.1:23338336-23338355 MS.gene86230:CDS 25.0%
! CTACCATGTTCAAATAATAT+AGG + chr5.1:23337676-23337695 MS.gene86230:intron 25.0%
! TATTAATAGATCATTCACTG+TGG - chr5.1:23336508-23336527 None:intergenic 25.0%
! TCATTTGTATAAGAAAGTGA+AGG - chr5.1:23336531-23336550 None:intergenic 25.0%
! TGAAAATTTATGCATCTAGA+TGG + chr5.1:23336223-23336242 MS.gene86230:CDS 25.0%
! TGAATTTGATGAAAATGGTT+TGG + chr5.1:23338341-23338360 MS.gene86230:CDS 25.0%
! TGATCTCATAATTCAAGTAA+GGG + chr5.1:23335265-23335284 MS.gene86230:CDS 25.0%
! TTACCAAATATTTCCAAGAA+CGG + chr5.1:23336246-23336265 MS.gene86230:CDS 25.0%
! TTCAACATTTATTGGAAGTT+AGG + chr5.1:23337235-23337254 MS.gene86230:intron 25.0%
!!! TAACATGTACTTTTAAGTTC+AGG + chr5.1:23337532-23337551 MS.gene86230:intron 25.0%
!!! TATGTGTGTTTTTTCTTTAC+AGG - chr5.1:23337099-23337118 None:intergenic 25.0%
!!! TTATGTGTTTTTTAACATGC+AGG + chr5.1:23337957-23337976 MS.gene86230:intron 25.0%
AAAGATGGAATTAGCAAGAA+GGG + chr5.1:23336461-23336480 MS.gene86230:CDS 30.0%
AACTTAGCATAGACCTTTAT+TGG + chr5.1:23336139-23336158 MS.gene86230:CDS 30.0%
AAGTAGATATCTTCTTCACA+AGG + chr5.1:23335289-23335308 MS.gene86230:CDS 30.0%
AATCAGGAAGTTGAACTATT+TGG - chr5.1:23335693-23335712 None:intergenic 30.0%
ACAATGATAGCAATCAAATC+TGG + chr5.1:23336165-23336184 MS.gene86230:CDS 30.0%
ACTTGAACAACAACTCTTAA+TGG - chr5.1:23338090-23338109 None:intergenic 30.0%
AGAATCAAATCAGGAAGTAA+AGG + chr5.1:23336093-23336112 MS.gene86230:CDS 30.0%
AGGATATGAAACAGAATTAG+TGG + chr5.1:23337177-23337196 MS.gene86230:intron 30.0%
ATACATGAATATGTGACTAC+AGG + chr5.1:23337892-23337911 MS.gene86230:intron 30.0%
ATGACTGAAGAAGTTGAAAA+AGG + chr5.1:23335826-23335845 MS.gene86230:CDS 30.0%
CAACTATATCAGTCAAGATA+AGG - chr5.1:23337299-23337318 None:intergenic 30.0%
CACAAACACAAATACAAACA+CGG - chr5.1:23335524-23335543 None:intergenic 30.0%
CATCATTATAGAGTTCATTG+AGG - chr5.1:23337512-23337531 None:intergenic 30.0%
CTATCATTGTTCTCCAATAA+AGG - chr5.1:23336155-23336174 None:intergenic 30.0%
CTTGAACAACAACTCTTAAT+GGG - chr5.1:23338089-23338108 None:intergenic 30.0%
GAAAGTTTGAGAATCAAATC+AGG + chr5.1:23336084-23336103 MS.gene86230:CDS 30.0%
GACTAATAAAAACTAGGCTA+TGG + chr5.1:23337698-23337717 MS.gene86230:intron 30.0%
GATCTCATAATTCAAGTAAG+GGG + chr5.1:23335266-23335285 MS.gene86230:CDS 30.0%
GTGATCTCATAATTCAAGTA+AGG + chr5.1:23335264-23335283 MS.gene86230:CDS 30.0%
TACCTTGAGATAAATGTCAA+TGG - chr5.1:23336877-23336896 None:intergenic 30.0%
TCTATCTTAATACTCGTTGA+GGG - chr5.1:23337006-23337025 None:intergenic 30.0%
TGATCATATCTTGCAAATTC+TGG - chr5.1:23336837-23336856 None:intergenic 30.0%
TTAATAGAAGGGATTAAGCA+CGG - chr5.1:23337033-23337052 None:intergenic 30.0%
TTGAATTACAAGAAAGTAGG+AGG + chr5.1:23336688-23336707 MS.gene86230:CDS 30.0%
TTTCAACTTCTTCAGTCATT+TGG - chr5.1:23335825-23335844 None:intergenic 30.0%
TTTCCGTTCTTGGAAATATT+TGG - chr5.1:23336252-23336271 None:intergenic 30.0%
!! AATGGTTTGGTAAATGATGA+TGG + chr5.1:23338354-23338373 MS.gene86230:CDS 30.0%
!! AGAGTTGAAGACTTTGATTT+TGG - chr5.1:23338294-23338313 None:intergenic 30.0%
!! GAAGAAAATTTTCTAGCATG+AGG + chr5.1:23337720-23337739 MS.gene86230:intron 30.0%
!! GTTCTTTTCTTTGTTTATGC+AGG + chr5.1:23335219-23335238 MS.gene86230:intron 30.0%
!! TGAAGAAGCATTGAGAATAT+TGG - chr5.1:23336436-23336455 None:intergenic 30.0%
!! TGTCATATTAGTGTTGTTTC+TGG + chr5.1:23335495-23335514 MS.gene86230:intron 30.0%
!! TGTGTTGTTGTATGTTTTGA+TGG - chr5.1:23338210-23338229 None:intergenic 30.0%
!! TTGGTAAATGATGATGGAAT+TGG + chr5.1:23338360-23338379 MS.gene86230:CDS 30.0%
!!! AGCATTTTTTTAGGTTGTGT+AGG - chr5.1:23338420-23338439 None:intergenic 30.0%
!!! TATTTTAAGGTCAAGAGAGT+TGG - chr5.1:23337207-23337226 None:intergenic 30.0%
AAACTTGATTCACAAGCTTG+AGG + chr5.1:23335849-23335868 MS.gene86230:CDS 35.0%
AATAGTTTCAATGCATCTGC+TGG - chr5.1:23335974-23335993 None:intergenic 35.0%
ACGTATTGTTACATTTGGGA+AGG + chr5.1:23335438-23335457 MS.gene86230:intron 35.0%
AGCCATTGACATTTATCTCA+AGG + chr5.1:23336872-23336891 MS.gene86230:CDS 35.0%
AGCTCTACTACAAGTATTCA+AGG + chr5.1:23338234-23338253 MS.gene86230:CDS 35.0%
ATGATAGCAATCAAATCTGG+TGG + chr5.1:23336168-23336187 MS.gene86230:CDS 35.0%
ATTAGTAGTTCGTTACGAGA+GGG - chr5.1:23337064-23337083 None:intergenic 35.0%
ATTCTGAGCATAATCTTTGC+AGG - chr5.1:23335648-23335667 None:intergenic 35.0%
ATTGAAAGTGGCAAAACTTG+TGG + chr5.1:23336737-23336756 MS.gene86230:CDS 35.0%
ATTTGTGTTTGTGTCTGTGT+CGG + chr5.1:23335531-23335550 MS.gene86230:intron 35.0%
CAAAGATGGAATTAGCAAGA+AGG + chr5.1:23336460-23336479 MS.gene86230:CDS 35.0%
CACACTATCAATTCATGAGA+AGG - chr5.1:23337425-23337444 None:intergenic 35.0%
CATTATAGAGTTCATTGAGG+TGG - chr5.1:23337509-23337528 None:intergenic 35.0%
CATTTATTGGAAGTTAGGTG+TGG + chr5.1:23337240-23337259 MS.gene86230:intron 35.0%
CTCAACGAGTATTAAGATAG+AGG + chr5.1:23337005-23337024 MS.gene86230:intron 35.0%
CTCTATCTTAATACTCGTTG+AGG - chr5.1:23337007-23337026 None:intergenic 35.0%
CTTCTTTCTATTTCCGTTCT+TGG - chr5.1:23336262-23336281 None:intergenic 35.0%
GCGAAATAACTTCAAAGCAT+AGG + chr5.1:23336325-23336344 MS.gene86230:CDS 35.0%
GTATTCAAGGTGAGGATAAT+TGG + chr5.1:23338247-23338266 MS.gene86230:CDS 35.0%
TACAGTCTATGATGTTGAGT+TGG + chr5.1:23336551-23336570 MS.gene86230:CDS 35.0%
TACTACAAGTATTCAAGGTG+AGG + chr5.1:23338239-23338258 MS.gene86230:CDS 35.0%
TCAAAAGTCTTCAACGATCT+CGG - chr5.1:23336633-23336652 None:intergenic 35.0%
TCAAATCAGGAAGTAAAGGA+TGG + chr5.1:23336097-23336116 MS.gene86230:CDS 35.0%
TCCAGATACTTTCTACACTT+CGG + chr5.1:23335104-23335123 MS.gene86230:CDS 35.0%
TGCTTCTTCATCTTCAAAGA+TGG + chr5.1:23336446-23336465 MS.gene86230:CDS 35.0%
TGTCACATAGTCATTCTAGA+AGG + chr5.1:23336031-23336050 MS.gene86230:CDS 35.0%
TTCAAAGCATAGGCTTCTAT+TGG + chr5.1:23336335-23336354 MS.gene86230:CDS 35.0%
TTGGAACAGTTCATGTTACA+AGG + chr5.1:23336585-23336604 MS.gene86230:CDS 35.0%
! AAATGTCAATGGCTCTATAG+AGG - chr5.1:23336866-23336885 None:intergenic 35.0%
! ACATAGTACTGGAACTATGA+TGG - chr5.1:23337402-23337421 None:intergenic 35.0%
! AGTTCAACTTCCTGATTTTC+CGG + chr5.1:23335696-23335715 MS.gene86230:CDS 35.0%
! ATAGTCATTCTAGAAGGATG+AGG + chr5.1:23336037-23336056 MS.gene86230:CDS 35.0%
! TAGATGATGCATCTCCTTTT+TGG + chr5.1:23335174-23335193 MS.gene86230:intron 35.0%
! TAGTCATTCTAGAAGGATGA+GGG + chr5.1:23336038-23336057 MS.gene86230:CDS 35.0%
!! AAAGCATTGCTTACAACACA+TGG + chr5.1:23338177-23338196 MS.gene86230:CDS 35.0%
!! ATGCTTTGAAGTTATTTCGC+TGG - chr5.1:23336325-23336344 None:intergenic 35.0%
!! ATTTGGTGATGAGATGAATC+AGG - chr5.1:23335676-23335695 None:intergenic 35.0%
!! CGTTGAAGACTTTTGAAAGA+AGG + chr5.1:23336637-23336656 MS.gene86230:CDS 35.0%
!! TGAAGACTTTTGAAAGAAGG+CGG + chr5.1:23336640-23336659 MS.gene86230:CDS 35.0%
!! TGAGTTGGTGATGACTATAT+TGG + chr5.1:23336566-23336585 MS.gene86230:CDS 35.0%
AAGAAGGGTAGGACTTCAAT+TGG + chr5.1:23336476-23336495 MS.gene86230:CDS 40.0%
AAGGTTCCTGCAAATCTCAT+TGG + chr5.1:23336192-23336211 MS.gene86230:CDS 40.0%
AAGTGGCAAAACTTGTGGAT+AGG + chr5.1:23336742-23336761 MS.gene86230:CDS 40.0%
AATCAGGAAGTAAAGGATGG+TGG + chr5.1:23336100-23336119 MS.gene86230:CDS 40.0%
ACCTCCTGTTAGAACTCATT+CGG + chr5.1:23337630-23337649 MS.gene86230:intron 40.0%
AGGGGGAAATCGTTAATAGA+AGG - chr5.1:23337045-23337064 None:intergenic 40.0%
AGTAAGTCTTCAGACCACAA+AGG + chr5.1:23335915-23335934 MS.gene86230:CDS 40.0%
ATCAGGAAGTAAAGGATGGT+GGG + chr5.1:23336101-23336120 MS.gene86230:CDS 40.0%
ATGGAATTAGCAAGAAGGGT+AGG + chr5.1:23336465-23336484 MS.gene86230:CDS 40.0%
CAGAACTGAACAAGAGTGAA+AGG + chr5.1:23337986-23338005 MS.gene86230:CDS 40.0%
CATAAGCATTTGGATAGCAG+GGG - chr5.1:23335625-23335644 None:intergenic 40.0%
CATCTCTTGCAACTTCTTGA+AGG - chr5.1:23336770-23336789 None:intergenic 40.0%
CATTTGTGGTCTGAAGACTT+GGG + chr5.1:23335943-23335962 MS.gene86230:CDS 40.0%
CCAAATGTAACAATACGTGC+CGG - chr5.1:23335436-23335455 None:intergenic 40.0%
CGGCACGTATTGTTACATTT+GGG + chr5.1:23335434-23335453 MS.gene86230:intron 40.0%
CTTGCAGCTACAATGTTGTA+AGG - chr5.1:23335784-23335803 None:intergenic 40.0%
CTTTCACTCTTGTTCAGTTC+TGG - chr5.1:23337988-23338007 None:intergenic 40.0%
GAGTTCATTGAGGTGGAAAT+CGG - chr5.1:23337502-23337521 None:intergenic 40.0%
GATTAGTAGTTCGTTACGAG+AGG - chr5.1:23337065-23337084 None:intergenic 40.0%
GCATAAGCATTTGGATAGCA+GGG - chr5.1:23335626-23335645 None:intergenic 40.0%
GGGGGAAATCGTTAATAGAA+GGG - chr5.1:23337044-23337063 None:intergenic 40.0%
GTGACAAACTCACCTTTGTA+GGG - chr5.1:23336017-23336036 None:intergenic 40.0%
TCATGAGAAGGACATAGTAC+TGG - chr5.1:23337413-23337432 None:intergenic 40.0%
TCTACACTTCGGAAGCTATA+AGG + chr5.1:23335115-23335134 MS.gene86230:CDS 40.0%
TCTTTGCAGGCATAAGCATT+TGG - chr5.1:23335635-23335654 None:intergenic 40.0%
TGAACTATGTGATGCTGAAG+AGG - chr5.1:23336715-23336734 None:intergenic 40.0%
TGACTGCAAAAAGCTCTCAA+TGG + chr5.1:23338029-23338048 MS.gene86230:CDS 40.0%
TGTGACAAACTCACCTTTGT+AGG - chr5.1:23336018-23336037 None:intergenic 40.0%
TGTGGTCTGAAGACTTACTA+TGG - chr5.1:23335914-23335933 None:intergenic 40.0%
TTAGTAGTTCGTTACGAGAG+GGG - chr5.1:23337063-23337082 None:intergenic 40.0%
TTCAATTCTTGCATCCTCCA+TGG + chr5.1:23335877-23335896 MS.gene86230:CDS 40.0%
TTGTGTGCTAAGTTCTGCTA+TGG + chr5.1:23335739-23335758 MS.gene86230:CDS 40.0%
TTTGAACATGGTAGCTCAGT+TGG - chr5.1:23337670-23337689 None:intergenic 40.0%
! AGCTTGCTTCCAACAGAAAA+AGG + chr5.1:23336369-23336388 MS.gene86230:CDS 40.0%
! GAGGATAATTGGAGTGTTTC+AGG + chr5.1:23338258-23338277 MS.gene86230:CDS 40.0%
! GTACTGACGCTTCAAATTGA+AGG + chr5.1:23335384-23335403 MS.gene86230:intron 40.0%
! GTTCAACTTCCTGATTTTCC+GGG + chr5.1:23335697-23335716 MS.gene86230:CDS 40.0%
! TGAGGCGTCACTATAATGAT+TGG + chr5.1:23337738-23337757 MS.gene86230:intron 40.0%
! TTGTGTAGGAAGAGCACAAA+TGG - chr5.1:23338406-23338425 None:intergenic 40.0%
!! GGTGTTGGCTCAAAATTCAA+CGG + chr5.1:23337809-23337828 MS.gene86230:intron 40.0%
!! TCCGAAGTGTAGAAAGTATC+TGG - chr5.1:23335108-23335127 None:intergenic 40.0%
!! TCTAAGAATGAAGCTTGCTG+AGG + chr5.1:23338311-23338330 MS.gene86230:CDS 40.0%
AAAGTCTTCAACGATCTCGG+AGG - chr5.1:23336630-23336649 None:intergenic 45.0%
AAGGGTAGGACTTCAATTGG+AGG + chr5.1:23336479-23336498 MS.gene86230:CDS 45.0%
AAGTCTTCAACGATCTCGGA+GGG - chr5.1:23336629-23336648 None:intergenic 45.0%
ACAAACTCACCTTTGTAGGG+TGG - chr5.1:23336014-23336033 None:intergenic 45.0%
AGCAATCAAATCTGGTGGCA+AGG + chr5.1:23336173-23336192 MS.gene86230:CDS 45.0%
ATTCTTGCATCCTCCATGGT+TGG + chr5.1:23335881-23335900 MS.gene86230:CDS 45.0%
CATTCACGCAGTCAGTTCAT+TGG + chr5.1:23337784-23337803 MS.gene86230:intron 45.0%
CCGGCACGTATTGTTACATT+TGG + chr5.1:23335433-23335452 MS.gene86230:intron 45.0%
CTATGGTATCCTTCCAACCA+TGG - chr5.1:23335897-23335916 None:intergenic 45.0%
GCATTTGTGGTCTGAAGACT+TGG + chr5.1:23335942-23335961 MS.gene86230:CDS 45.0%
GGCATAAGCATTTGGATAGC+AGG - chr5.1:23335627-23335646 None:intergenic 45.0%
GTAGCTCAGTGATACGAGTT+GGG + chr5.1:23336979-23336998 MS.gene86230:intron 45.0%
GTCAAGATAAGGAGTCAGTC+AGG - chr5.1:23337288-23337307 None:intergenic 45.0%
GTCAGTCAGGCCAAAATTTC+TGG - chr5.1:23337275-23337294 None:intergenic 45.0%
GTCAGTTCATTGGTAGGTGT+TGG + chr5.1:23337794-23337813 MS.gene86230:intron 45.0%
GTCTGAGTCTAACTCAGACT+CGG + chr5.1:23336299-23336318 MS.gene86230:CDS 45.0%
GTCTGAGTTAGACTCAGACT+CGG - chr5.1:23336298-23336317 None:intergenic 45.0%
TAGTAGTTCGTTACGAGAGG+GGG - chr5.1:23337062-23337081 None:intergenic 45.0%
TGTAGCTCAGTGATACGAGT+TGG + chr5.1:23336978-23336997 MS.gene86230:intron 45.0%
! ACCGAATGAGTTCTAACAGG+AGG - chr5.1:23337634-23337653 None:intergenic 45.0%
! AGTCAGGCCAAAATTTCTGG+TGG - chr5.1:23337272-23337291 None:intergenic 45.0%
! ATTTTGTGCTGCATGCATGC+AGG - chr5.1:23338058-23338077 None:intergenic 45.0%
! GAAACAGCGCCTTTTTCTGT+TGG - chr5.1:23336381-23336400 None:intergenic 45.0%
! GACACCGAATGAGTTCTAAC+AGG - chr5.1:23337637-23337656 None:intergenic 45.0%
! GACTGACCCACCAGAAATTT+TGG + chr5.1:23337262-23337281 MS.gene86230:intron 45.0%
! TGAGTTCTAACAGGAGGTTC+TGG - chr5.1:23337628-23337647 None:intergenic 45.0%
! TGGTGATGAGATGAATCAGG+AGG - chr5.1:23335673-23335692 None:intergenic 45.0%
!! GTCAGGCCAAAATTTCTGGT+GGG - chr5.1:23337271-23337290 None:intergenic 45.0%
AGTCTTGAGCCACCCTACAA+AGG + chr5.1:23336002-23336021 MS.gene86230:CDS 50.0%
CACGCAGTCAGTTCATTGGT+AGG + chr5.1:23337788-23337807 MS.gene86230:intron 50.0%
CCACAAATGCAACGCCTTTG+TGG - chr5.1:23335932-23335951 None:intergenic 50.0%
GTCAGACACTTACACGACAC+CGG + chr5.1:23335414-23335433 MS.gene86230:intron 50.0%
GTGTCGCCAATGAGATTTGC+AGG - chr5.1:23336201-23336220 None:intergenic 50.0%
TCAAATGCTTCCACTCCACC+CGG - chr5.1:23335718-23335737 None:intergenic 50.0%
TGAAAGAAGGCGGTCACGTT+CGG + chr5.1:23336650-23336669 MS.gene86230:CDS 50.0%
TGGTATCCTTCCAACCATGG+AGG - chr5.1:23335894-23335913 None:intergenic 50.0%
TTGCATCCTCCATGGTTGGA+AGG + chr5.1:23335885-23335904 MS.gene86230:CDS 50.0%
! ACATGGTAGCTCAGTTGGTG+AGG - chr5.1:23337665-23337684 None:intergenic 50.0%
! CAACTTCCTGATTTTCCGGG+TGG + chr5.1:23335700-23335719 MS.gene86230:CDS 50.0%
!! CCACAAAGGCGTTGCATTTG+TGG + chr5.1:23335929-23335948 MS.gene86230:CDS 50.0%
CAAGCTAGAGCAGCAGCATC+TGG + chr5.1:23338123-23338142 MS.gene86230:CDS 55.0%
GAACTGACTGCGTGAATGCG+TGG - chr5.1:23337782-23337801 None:intergenic 55.0%
TCCACTCCACCCGGAAAATC+AGG - chr5.1:23335709-23335728 None:intergenic 55.0%
! AACGATCTCGGAGGGCTAGT+TGG - chr5.1:23336621-23336640 None:intergenic 55.0%
! TCCTGATTTTCCGGGTGGAG+TGG + chr5.1:23335705-23335724 MS.gene86230:CDS 55.0%
GCTAGAGCAGCAGCATCTGG+TGG + chr5.1:23338126-23338145 MS.gene86230:CDS 60.0%
!! GATCTCGGAGGGCTAGTTGG+TGG - chr5.1:23336618-23336637 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 23335078 23338485 23335078 ID=MS.gene86230
chr5.1 mRNA 23335078 23338485 23335078 ID=MS.gene86230.t1;Parent=MS.gene86230
chr5.1 exon 23335078 23335136 23335078 ID=MS.gene86230.t1.exon1;Parent=MS.gene86230.t1
chr5.1 CDS 23335078 23335136 23335078 ID=cds.MS.gene86230.t1;Parent=MS.gene86230.t1
chr5.1 exon 23335241 23335310 23335241 ID=MS.gene86230.t1.exon2;Parent=MS.gene86230.t1
chr5.1 CDS 23335241 23335310 23335241 ID=cds.MS.gene86230.t1;Parent=MS.gene86230.t1
chr5.1 exon 23335619 23336893 23335619 ID=MS.gene86230.t1.exon3;Parent=MS.gene86230.t1
chr5.1 CDS 23335619 23336893 23335619 ID=cds.MS.gene86230.t1;Parent=MS.gene86230.t1
chr5.1 exon 23337979 23338485 23337979 ID=MS.gene86230.t1.exon4;Parent=MS.gene86230.t1
chr5.1 CDS 23337979 23338485 23337979 ID=cds.MS.gene86230.t1;Parent=MS.gene86230.t1
Gene Sequence

>MS.gene86230

ATGAAGTTTATGAAACTTGGATCTCGTCCAGATACTTTCTACACTTCGGAAGCTATAAGGTAAGACATTAACAAAAATAAATATCATCTTTGTTCTTAGATGATGCATCTCCTTTTTGGATTGTTTAATTTTGTAACATGAGTTCTTTTCTTTGTTTATGCAGGACAATCTCTTCTGAAGTTTCTAGTGATCTCATAATTCAAGTAAGGGGAAGTAGATATCTTCTTCACAAGGTATGTTTCTTGAAAGTAGAATTAACTCATCAAGTTTCTTTTATTTATTTATTGAATTGTATATTGCTATGTAGTACTGACGCTTCAAATTGAAGGCTTCTCAGTCAGACACTTACACGACACCGGCACGTATTGTTACATTTGGGAAGGAAAAAAAAATTAAAAATTAATATCCGTATCAATGTGTCATATTAGTGTTGTTTCTGGTGTCCGTGTTTGTATTTGTGTTTGTGTCTGTGTCGGTCCTTCATAAGTATATAATTTTTTACTTTGGTAACTTATCTGAAGTTCATGCTCATGTTTTTCAGTTTCCCCTGCTATCCAAATGCTTATGCCTGCAAAGATTATGCTCAGAATCACCTCCTGATTCATCTCATCACCAAATAGTTCAACTTCCTGATTTTCCGGGTGGAGTGGAAGCATTTGAGTTGTGTGCTAAGTTCTGCTATGGAATCACAATTACTCTAAGTCCTTACAACATTGTAGCTGCAAGATGTGCTGCTGAATATCTCCAAATGACTGAAGAAGTTGAAAAAGGAAACTTGATTCACAAGCTTGAGGTTTTTTTCAATTCTTGCATCCTCCATGGTTGGAAGGATACCATAGTAAGTCTTCAGACCACAAAGGCGTTGCATTTGTGGTCTGAAGACTTGGGAATTACCAGCAGATGCATTGAAACTATTGCGTCAAAAGTCTTGAGCCACCCTACAAAGGTGAGTTTGTCACATAGTCATTCTAGAAGGATGAGGGATGACATATCTTGCAATGATACAGAAAGTTTGAGAATCAAATCAGGAAGTAAAGGATGGTGGGCTGAAGATTTAGCAGAACTTAGCATAGACCTTTATTGGAGAACAATGATAGCAATCAAATCTGGTGGCAAGGTTCCTGCAAATCTCATTGGCGACACATTGAAAATTTATGCATCTAGATGGTTACCAAATATTTCCAAGAACGGAAATAGAAAGAAGAATGTTTCGCTAGCCGAGTCTGAGTCTAACTCAGACTCGGCCAGCGAAATAACTTCAAAGCATAGGCTTCTATTGGAGTCAATAGTAAGCTTGCTTCCAACAGAAAAAGGCGCTGTTTCTTGTAGTTTTCTTTTGAAACTATTGAAAGCATCCAATATTCTCAATGCTTCTTCATCTTCAAAGATGGAATTAGCAAGAAGGGTAGGACTTCAATTGGAGGAAGCCACAGTGAATGATCTATTAATACCTTCACTTTCTTATACAAATGATACAGTCTATGATGTTGAGTTGGTGATGACTATATTGGAACAGTTCATGTTACAAGGACAAAGTCCACCAACTAGCCCTCCGAGATCGTTGAAGACTTTTGAAAGAAGGCGGTCACGTTCGGCTGAGAATATTAACTTTGAATTACAAGAAAGTAGGAGGTCCTCTTCAGCATCACATAGTTCAAAATTGAAAGTGGCAAAACTTGTGGATAGGTACCTTCAAGAAGTTGCAAGAGATGTGAATTTTTCATTGTCAAAATTCATTGCTCTTGCTGATATTATACCAGAATTTGCAAGATATGATCATGATGACCTCTATAGAGCCATTGACATTTATCTCAAGGTAAAATTCATTCCTATAAAATGTTTATTGTATTTTTTTTAAGTCACTCCATTTTATACCTTGATAAAAATATATCTCAGATGGTGTAGCTCAGTGATACGAGTTGGGACCCTCAACGAGTATTAAGATAGAGGCTCCGTGCTTAATCCCTTCTATTAACGATTTCCCCCTCTCGTAACGAACTACTAATCACTAACATTTGCCTGTAAAGAAAAAACACACATAAAAAACATGTAAAGAAAAAAAGTGGTTGTACATAATTCATACTTCACTTTCAAATTTTAGGATATGAAACAGAATTAGTGGATAACCAACTCTCTTGACCTTAAAATATTGTCTTATTCAACATTTATTGGAAGTTAGGTGTGGACTGACCCACCAGAAATTTTGGCCTGACTGACTCCTTATCTTGACTGATATAGTTGCAGTATGTACTCAGATAATTTCATAACTCATTTGGCTAAAAACGTGAAGAAAAATTTCTTGTCAAAACTGTTACAGAGTTCCATCATAGTTCCAGTACTATGTCCTTCTCATGAATTGATAGTGTGTCTCTTCAATTTTACATCACAAGTTTTGTCCATTTATGAATTCACTCTATTTAGCCGATTTCCACCTCAATGAACTCTATAATGATGTAACATGTACTTTTAAGTTCAGGTTTTTTTATTTTTATTTTGGCTATACAAATTAGTAAATTTTAATTTAAGTTTTTTTTTCAAGGATTGAATCCAGAACCTCCTGTTAGAACTCATTCGGTGTCTCTTACCTCACCAACTGAGCTACCATGTTCAAATAATATAGGACTAATAAAAACTAGGCTATGGAAGAAAATTTTCTAGCATGAGGCGTCACTATAATGATTGGACTTACTCTTGTCAAATTCCACGCATTCACGCAGTCAGTTCATTGGTAGGTGTTGGCTCAAAATTCAACGGTCCATATTTCAAGCTCGTTATTTTAGAGTTCAAAAAATTTCTTAACTGATGTGCGTGCGAATACATGAATATGTGACTACAGGCTGCAGCAATAGATTTATTATTATTATTATCATTGCAAAAATTTATGTGTTTTTTAACATGCAGGCTCATCCAGAACTGAACAAGAGTGAAAGGAAAAGACTATGTAGAATTCTTGACTGCAAAAAGCTCTCAATGGAAGCCTGCATGCATGCAGCACAAAATGAGCTACTCCCATTAAGAGTTGTTGTTCAAGTTCTGTTCTTTGAGCAAGCTAGAGCAGCAGCATCTGGTGGCAAAGTAACAGAACTACAAAGCAATATCAAAGCATTGCTTACAACACATGGTATTGATCCATCAAAACATACAACAACACAGTTAAGCTCTACTACAAGTATTCAAGGTGAGGATAATTGGAGTGTTTCAGGTTTCAAGTCACCAAAATCAAAGTCTTCAACTCTAAGAATGAAGCTTGCTGAGGATCATGATGAATTTGATGAAAATGGTTTGGTAAATGATGATGGAATTGGAAGAAACTCTAAATTTAAGTCCATTTGTGCTCTTCCTACACAACCTAAAAAAATGCTTAGTAAATTGTGGTCTACTAATAGAACTGCTACTGAAAAAAATTGA
Protein sequence

>MS.gene86230.t1

MKFMKLGSRPDTFYTSEAIRTISSEVSSDLIIQVRGSRYLLHKFPLLSKCLCLQRLCSESPPDSSHHQIVQLPDFPGGVEAFELCAKFCYGITITLSPYNIVAARCAAEYLQMTEEVEKGNLIHKLEVFFNSCILHGWKDTIVSLQTTKALHLWSEDLGITSRCIETIASKVLSHPTKVSLSHSHSRRMRDDISCNDTESLRIKSGSKGWWAEDLAELSIDLYWRTMIAIKSGGKVPANLIGDTLKIYASRWLPNISKNGNRKKNVSLAESESNSDSASEITSKHRLLLESIVSLLPTEKGAVSCSFLLKLLKASNILNASSSSKMELARRVGLQLEEATVNDLLIPSLSYTNDTVYDVELVMTILEQFMLQGQSPPTSPPRSLKTFERRRSRSAENINFELQESRRSSSASHSSKLKVAKLVDRYLQEVARDVNFSLSKFIALADIIPEFARYDHDDLYRAIDIYLKAHPELNKSERKRLCRILDCKKLSMEACMHAAQNELLPLRVVVQVLFFEQARAAASGGKVTELQSNIKALLTTHGIDPSKHTTTQLSSTTSIQGEDNWSVSGFKSPKSKSSTLRMKLAEDHDEFDENGLVNDDGIGRNSKFKSICALPTQPKKMLSKLWSTNRTATEKN