AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86383

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86383.t1 MTR_2g103600 97.468 316 8 0 1 316 1 316 0.0 625
MS.gene86383.t1 MTR_4g021755 56.184 283 114 5 30 310 41 315 2.20e-101 303
MS.gene86383.t1 MTR_7g498280 56.357 291 118 4 15 304 20 302 1.09e-100 299
MS.gene86383.t1 MTR_7g061780 54.255 282 120 4 30 310 25 298 1.76e-95 286
MS.gene86383.t1 MTR_2g097210 55.636 275 114 3 30 302 43 311 2.48e-90 275
MS.gene86383.t1 MTR_2g097210 56.818 264 106 3 30 291 43 300 2.92e-89 269
MS.gene86383.t1 MTR_1g101310 48.366 306 138 3 3 304 5 294 1.33e-88 268
MS.gene86383.t1 MTR_3g073530 43.985 266 141 3 33 297 42 300 1.69e-55 185
MS.gene86383.t1 MTR_6g089790 68.421 57 16 1 191 247 1 55 6.01e-17 74.3
MS.gene86383.t1 MTR_2g088315 46.053 76 36 1 107 182 13 83 1.63e-15 70.9
MS.gene86383.t1 MTR_2g088290 30.435 207 90 8 51 252 1 158 2.08e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86383.t1 AT1G52580 60.797 301 109 7 1 299 1 294 4.19e-120 348
MS.gene86383.t1 AT2G29050 61.011 277 99 4 27 302 50 318 1.26e-108 321
MS.gene86383.t1 AT2G29050 55.932 295 93 5 27 292 50 336 1.55e-102 304
MS.gene86383.t1 AT1G63120 57.312 253 96 4 28 278 28 270 1.21e-97 291
MS.gene86383.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene86383.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene86383.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene86383.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene86383.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene86383.t1 AT5G07250 50.649 308 132 5 11 306 25 324 6.12e-90 272
MS.gene86383.t1 AT3G53780 50.554 271 122 4 30 300 63 321 2.44e-84 259
MS.gene86383.t1 AT3G53780 51.145 262 116 4 30 291 63 312 2.97e-83 254
MS.gene86383.t1 AT4G23070 51.971 279 123 5 27 304 25 293 8.39e-75 233
MS.gene86383.t1 AT1G12750 55.615 187 73 4 29 211 16 196 9.79e-66 206
MS.gene86383.t1 AT1G12750 56.983 179 69 3 29 205 16 188 1.20e-65 205
MS.gene86383.t1 AT3G53780 50.000 206 94 2 95 300 1 197 3.82e-64 204
MS.gene86383.t1 AT1G77860 41.132 265 136 5 33 290 48 299 1.89e-59 194
MS.gene86383.t1 AT1G77860 41.132 265 136 5 33 290 48 299 2.71e-59 195
MS.gene86383.t1 AT5G07250 53.140 207 79 4 11 205 25 225 2.60e-58 189
MS.gene86383.t1 AT1G25290 34.375 160 91 5 103 255 161 313 1.75e-14 73.6
MS.gene86383.t1 AT1G25290 34.416 154 92 4 103 255 161 306 3.24e-14 72.8
MS.gene86383.t1 AT5G38510 29.193 161 105 4 96 254 219 372 1.09e-11 65.5
MS.gene86383.t1 AT5G38510 29.193 161 105 4 96 254 219 372 1.09e-11 65.5
MS.gene86383.t1 AT5G38510 29.193 161 105 4 96 254 219 372 1.09e-11 65.5

Find 80 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCTTTGGTATCAATATT+TGG 0.097106 2.3:+1437284 MS.gene86383:CDS
GGAGTTCTATATTTGCTTTC+TGG 0.112592 2.3:+1438187 MS.gene86383:CDS
GTTCGCCTTGAGAATGAATT+TGG 0.205071 2.3:+1438044 MS.gene86383:CDS
TCTCATCATTGGCCTAAATT+TGG 0.218525 2.3:+1438737 MS.gene86383:CDS
CTATATTTGCTTTCTGGCTT+TGG 0.237472 2.3:+1438193 MS.gene86383:CDS
TTGGATTTGGCAGTGAAAAT+TGG 0.260847 2.3:+1438166 MS.gene86383:intron
GCACCAACTGATACGGTATT+TGG 0.268428 2.3:-1438259 None:intergenic
TTGGACTATCTATTTAAATA+AGG 0.284285 2.3:+1438336 MS.gene86383:CDS
ATACACGAACATTGCAATAT+TGG 0.288693 2.3:-1437217 None:intergenic
TTCTGCAGGTTGAGGGTATT+AGG 0.315192 2.3:+1437900 MS.gene86383:intron
TAAATCCAACAGCCAAATTT+AGG 0.316777 2.3:-1438749 None:intergenic
TGCCTTTGGTATCAATATTT+GGG 0.351007 2.3:+1437285 MS.gene86383:CDS
AGAAAGTAGGCGGTGGTGGC+GGG 0.367689 2.3:-1437140 None:intergenic
TCAGTCATTACGCTACTTAT+CGG 0.370474 2.3:+1438948 MS.gene86383:CDS
AAACGTAAATCCAAGTATAA+AGG 0.381415 2.3:+1438900 MS.gene86383:CDS
TAGAAAGTAGGCGGTGGTGG+CGG 0.386984 2.3:-1437141 None:intergenic
CATATAGGAGGATTCCTATC+AGG 0.389877 2.3:+1438795 MS.gene86383:CDS
ACCGTATCAGTTGGTGCATC+GGG 0.403381 2.3:+1438265 MS.gene86383:CDS
GAATAAAAGACTAAACATAT+TGG 0.408803 2.3:-1438015 None:intergenic
TATTTGCTTTCTGGCTTTGG+TGG 0.424233 2.3:+1438196 MS.gene86383:CDS
ACTTGCATGTTTCTTCATGC+AGG 0.433987 2.3:+1437975 MS.gene86383:CDS
AGGCTATCAGTATTTCTTCC+TGG 0.441269 2.3:+1438920 MS.gene86383:CDS
ACTTTCAGTATTCGCTAAGA+AGG 0.444351 2.3:-1439329 None:intergenic
GTTTACATTCTGCAGGTTGA+GGG 0.447403 2.3:+1437893 MS.gene86383:intron
GAAAGTAGGCGGTGGTGGCG+GGG 0.449589 2.3:-1437139 None:intergenic
ATTTAAATAGATAGTCCAAT+TGG 0.450252 2.3:-1438332 None:intergenic
CAGTCATTACGCTACTTATC+GGG 0.457261 2.3:+1438949 MS.gene86383:CDS
ATAACGAGCCGCCTCAATAT+CGG 0.458145 2.3:-1437115 None:intergenic
ATACTGATAGCCTTTATACT+TGG 0.460587 2.3:-1438910 None:intergenic
TTGTTCACATAACCAAATTG+AGG 0.462978 2.3:-1438849 None:intergenic
ATTGGCCTAAATTTGGCTGT+TGG 0.471687 2.3:+1438744 MS.gene86383:CDS
AAAGTAGGCGGTGGTGGCGG+GGG 0.473559 2.3:-1437138 None:intergenic
TCATCTTCATTCAAATAGCC+AGG 0.474228 2.3:-1437261 None:intergenic
TTAGTGAGCTTCTAACCAAT+TGG 0.474947 2.3:+1438317 MS.gene86383:CDS
TACCGTATCAGTTGGTGCAT+CGG 0.475291 2.3:+1438264 MS.gene86383:CDS
ACAAGTCTACTTCTCATCAT+TGG 0.480296 2.3:+1438726 MS.gene86383:CDS
TTCAATGAAAACCCTCTCCT+TGG 0.483625 2.3:+1437327 MS.gene86383:CDS
ATGTAGAAAGTAGGCGGTGG+TGG 0.484370 2.3:-1437144 None:intergenic
TGGTGGGTGAAAATGAAGTA+TGG 0.484851 2.3:+1437943 MS.gene86383:CDS
TGAATGAAGATGATTGCCTT+TGG 0.484862 2.3:+1437271 MS.gene86383:CDS
TCATTTGACAGATATGCCTA+TGG 0.495016 2.3:+1439265 MS.gene86383:intron
TGTTCACATAACCAAATTGA+GGG 0.495056 2.3:-1438848 None:intergenic
CGCCAACACAATGGTTCTCA+TGG 0.513113 2.3:+1437172 MS.gene86383:CDS
GATTCTCACGTGCGGACAGA+AGG 0.516174 2.3:-1437351 None:intergenic
AAAATCCAAATTCATTCTCA+AGG 0.516279 2.3:-1438049 None:intergenic
GTGGACAATTCAGCTCATAT+AGG 0.523918 2.3:+1438780 MS.gene86383:CDS
AACACAATGGTTCTCATGGT+TGG 0.531112 2.3:+1437176 MS.gene86383:CDS
GGACAGAAGGGCCAAGGAGA+GGG 0.532109 2.3:-1437338 None:intergenic
CATGAGAACCATTGTGTTGG+CGG 0.546872 2.3:-1437171 None:intergenic
ACCCGATGCACCAACTGATA+CGG 0.550141 2.3:-1438266 None:intergenic
GTCGCCGTTCTCCGATATTG+AGG 0.551647 2.3:+1437104 MS.gene86383:CDS
GGTATTAGGAGCTCTTGAAA+AGG 0.553959 2.3:+1437914 MS.gene86383:CDS
TTCCCAAATATTGATACCAA+AGG 0.563541 2.3:-1437287 None:intergenic
ACGTGCGGACAGAAGGGCCA+AGG 0.568851 2.3:-1437344 None:intergenic
ATGTATATCAATGATTGCCC+TGG 0.571703 2.3:+1437243 MS.gene86383:CDS
AACCATGAGAACCATTGTGT+TGG 0.576399 2.3:-1437174 None:intergenic
TCTTGAAAAGGACCTTGTGG+TGG 0.579909 2.3:+1437926 MS.gene86383:CDS
GTACAATGTAGCTAAGCCAT+AGG 0.586121 2.3:-1439281 None:intergenic
GGCGGTGGCATGTAGAAAGT+AGG 0.588387 2.3:-1437153 None:intergenic
GACAATTCAGCTCATATAGG+AGG 0.594629 2.3:+1438783 MS.gene86383:CDS
GGTTAGAAGCTCACTAAGCA+TGG 0.598778 2.3:-1438311 None:intergenic
GCGTAATGACTGACAAGACC+AGG 0.609130 2.3:-1438938 None:intergenic
ATTCTCACGTGCGGACAGAA+GGG 0.610517 2.3:-1437350 None:intergenic
CGGACAGAAGGGCCAAGGAG+AGG 0.611548 2.3:-1437339 None:intergenic
CATCTTCATTCAAATAGCCA+GGG 0.618324 2.3:-1437260 None:intergenic
GCCGCCTCAATATCGGAGAA+CGG 0.618640 2.3:-1437108 None:intergenic
GAGAACCATTGTGTTGGCGG+TGG 0.622152 2.3:-1437168 None:intergenic
GCACCAAATACCGTATCAGT+TGG 0.622766 2.3:+1438256 MS.gene86383:CDS
GGCTTAGCTACATTGTACAT+AGG 0.638607 2.3:+1439286 MS.gene86383:CDS
AGCTCTTGAAAAGGACCTTG+TGG 0.646384 2.3:+1437923 MS.gene86383:CDS
TGAGCTGAATTGTCCACATG+AGG 0.649223 2.3:-1438774 None:intergenic
GGCATGTAGAAAGTAGGCGG+TGG 0.649400 2.3:-1437147 None:intergenic
CTTGAAAAGGACCTTGTGGT+GGG 0.656591 2.3:+1437927 MS.gene86383:CDS
AACAACAAATATATACCACC+AGG 0.669042 2.3:+1438867 MS.gene86383:CDS
GCCGTTCTCCGATATTGAGG+CGG 0.669086 2.3:+1437107 MS.gene86383:CDS
ACATGCCACCGCCAACACAA+TGG 0.675117 2.3:+1437163 MS.gene86383:CDS
TGTTGGATTTATACCTCATG+TGG 0.677933 2.3:+1438761 MS.gene86383:CDS
GCATTAAAATAACAAAACCA+AGG 0.678293 2.3:-1438824 None:intergenic
GGTGGCATGTAGAAAGTAGG+CGG 0.681757 2.3:-1437150 None:intergenic
TGGGTGAAAATGAAGTATGG+CGG 0.777515 2.3:+1437946 MS.gene86383:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAAAAAAAAATACATA+GGG + chr2.3:1438393-1438412 MS.gene86383:intron 10.0%
!! TTACTTATTATATTTATTCT+TGG + chr2.3:1438147-1438166 MS.gene86383:intron 10.0%
!! AATTTGAATTTAATCTGTAT+TGG + chr2.3:1439212-1439231 MS.gene86383:intron 15.0%
!! ATTATATTTATTCTTGGATT+TGG + chr2.3:1438153-1438172 MS.gene86383:intron 15.0%
!! GAAAGAAAAAAAAAATACAT+AGG + chr2.3:1438392-1438411 MS.gene86383:intron 15.0%
!! TTTATGTTCAATTATAATGA+TGG + chr2.3:1439189-1439208 MS.gene86383:intron 15.0%
!! AAAAAAAAAATACATAGGGT+GGG + chr2.3:1438397-1438416 MS.gene86383:intron 20.0%
!! ATTTAAATAGATAGTCCAAT+TGG - chr2.3:1438335-1438354 None:intergenic 20.0%
!! GAATAAAAGACTAAACATAT+TGG - chr2.3:1438018-1438037 None:intergenic 20.0%
!! TCAAAGTTAATCTTGTAAAT+TGG + chr2.3:1438489-1438508 MS.gene86383:intron 20.0%
!! TTGGACTATCTATTTAAATA+AGG + chr2.3:1438336-1438355 MS.gene86383:CDS 20.0%
!!! ATGTTTAGTCTTTTATTCAT+AGG + chr2.3:1438020-1438039 MS.gene86383:CDS 20.0%
!!! CAAACGTTTTTAATAATTTG+AGG + chr2.3:1439098-1439117 MS.gene86383:intron 20.0%
!!! CATCATCATTTTAAATATCA+TGG + chr2.3:1437407-1437426 MS.gene86383:intron 20.0%
!!! CTCAATTTTTTGTTATATTG+CGG - chr2.3:1437632-1437651 None:intergenic 20.0%
!!! TTGTTATCTATTTTGCATTT+GGG + chr2.3:1438223-1438242 MS.gene86383:CDS 20.0%
!!! TTTGTTATCTATTTTGCATT+TGG + chr2.3:1438222-1438241 MS.gene86383:CDS 20.0%
! AAAATCCAAATTCATTCTCA+AGG - chr2.3:1438052-1438071 None:intergenic 25.0%
! AAACGTAAATCCAAGTATAA+AGG + chr2.3:1438900-1438919 MS.gene86383:CDS 25.0%
! ATTACATCAAAAGTTAAAGG+AGG - chr2.3:1439242-1439261 None:intergenic 25.0%
! CGCAATATAACAAAAAATTG+AGG + chr2.3:1437630-1437649 MS.gene86383:intron 25.0%
! GAAAAAAAAAATACATAGGG+TGG + chr2.3:1438396-1438415 MS.gene86383:intron 25.0%
! GCAATTACATCAAAAGTTAA+AGG - chr2.3:1439245-1439264 None:intergenic 25.0%
! GCATTAAAATAACAAAACCA+AGG - chr2.3:1438827-1438846 None:intergenic 25.0%
! GCTTTCTCATTAAAATATCA+TGG + chr2.3:1437453-1437472 MS.gene86383:intron 25.0%
! TGCCGAAATATAAAAAATTG+CGG + chr2.3:1437702-1437721 MS.gene86383:intron 25.0%
!! ATATTGGCTAAAAAAATGAG+TGG - chr2.3:1437204-1437223 None:intergenic 25.0%
!! TTTTATATTTCGGCAAAATG+CGG - chr2.3:1437697-1437716 None:intergenic 25.0%
!!! ATCTTTTTACTTTTGAACCA+AGG + chr2.3:1438646-1438665 MS.gene86383:intron 25.0%
!!! CACCGCAATTTTTTATATTT+CGG - chr2.3:1437707-1437726 None:intergenic 25.0%
!!! TGAAATGATAGTGATTTGTT+TGG - chr2.3:1439044-1439063 None:intergenic 25.0%
!!! TTTGTCAATTGGTTGTTTTA+GGG + chr2.3:1437863-1437882 MS.gene86383:intron 25.0%
!!! TTTTGTCAATTGGTTGTTTT+AGG + chr2.3:1437862-1437881 MS.gene86383:intron 25.0%
AAACAACCAATTGACAAAAG+AGG - chr2.3:1437861-1437880 None:intergenic 30.0%
AACAACAAATATATACCACC+AGG + chr2.3:1438867-1438886 MS.gene86383:CDS 30.0%
ATAATGTTCAACAATGTACC+TGG + chr2.3:1437431-1437450 MS.gene86383:intron 30.0%
ATACACGAACATTGCAATAT+TGG - chr2.3:1437220-1437239 None:intergenic 30.0%
ATACTGATAGCCTTTATACT+TGG - chr2.3:1438913-1438932 None:intergenic 30.0%
ATTTGAGGACTTTGATTGAT+TGG + chr2.3:1439113-1439132 MS.gene86383:intron 30.0%
CCATAAGCTTGATTATAAAG+AGG + chr2.3:1438536-1438555 MS.gene86383:intron 30.0%
CCTCTTTATAATCAAGCTTA+TGG - chr2.3:1438539-1438558 None:intergenic 30.0%
CTTCTATTGTCTTACTTAGA+TGG + chr2.3:1437750-1437769 MS.gene86383:intron 30.0%
TAAATCCAACAGCCAAATTT+AGG - chr2.3:1438752-1438771 None:intergenic 30.0%
TCAAAAACACAGAGACAATT+TGG + chr2.3:1438576-1438595 MS.gene86383:intron 30.0%
TGCCTTTGGTATCAATATTT+GGG + chr2.3:1437285-1437304 MS.gene86383:CDS 30.0%
TGTTCACATAACCAAATTGA+GGG - chr2.3:1438851-1438870 None:intergenic 30.0%
TTATTGTGTTTACATTCTGC+AGG + chr2.3:1437886-1437905 MS.gene86383:intron 30.0%
TTCCCAAATATTGATACCAA+AGG - chr2.3:1437290-1437309 None:intergenic 30.0%
TTGCCTTTGGTATCAATATT+TGG + chr2.3:1437284-1437303 MS.gene86383:CDS 30.0%
TTGTTCACATAACCAAATTG+AGG - chr2.3:1438852-1438871 None:intergenic 30.0%
! TTTCATCCTCTTTTGTCAAT+TGG + chr2.3:1437852-1437871 MS.gene86383:intron 30.0%
!! AATGATAGTGATTTGTTTGG+CGG - chr2.3:1439041-1439060 None:intergenic 30.0%
!!! GATATTTTAATGAGAAAGCC+AGG - chr2.3:1437452-1437471 None:intergenic 30.0%
AACCAAGGAAAAATCCTGAT+AGG - chr2.3:1438812-1438831 None:intergenic 35.0%
ACAAGTCTACTTCTCATCAT+TGG + chr2.3:1438726-1438745 MS.gene86383:CDS 35.0%
ACTTTCAGTATTCGCTAAGA+AGG - chr2.3:1439332-1439351 None:intergenic 35.0%
ACTTTGAACTGTACTGTGTA+AGG - chr2.3:1438476-1438495 None:intergenic 35.0%
ATGTATATCAATGATTGCCC+TGG + chr2.3:1437243-1437262 MS.gene86383:CDS 35.0%
ATGTGAGAGAGAAATTGAGA+GGG - chr2.3:1437381-1437400 None:intergenic 35.0%
ATTATGTCAAATGCATGTCC+TGG + chr2.3:1437555-1437574 MS.gene86383:intron 35.0%
CATCTTCATTCAAATAGCCA+GGG - chr2.3:1437263-1437282 None:intergenic 35.0%
CTTTGAACTGTACTGTGTAA+GGG - chr2.3:1438475-1438494 None:intergenic 35.0%
GGAGTTCTATATTTGCTTTC+TGG + chr2.3:1438187-1438206 MS.gene86383:CDS 35.0%
TATGTGAGAGAGAAATTGAG+AGG - chr2.3:1437382-1437401 None:intergenic 35.0%
TCAGTCATTACGCTACTTAT+CGG + chr2.3:1438948-1438967 MS.gene86383:CDS 35.0%
TCATCTTCATTCAAATAGCC+AGG - chr2.3:1437264-1437283 None:intergenic 35.0%
TCATTTGACAGATATGCCTA+TGG + chr2.3:1439265-1439284 MS.gene86383:intron 35.0%
TGAATGAAGATGATTGCCTT+TGG + chr2.3:1437271-1437290 MS.gene86383:CDS 35.0%
TGTTGGATTTATACCTCATG+TGG + chr2.3:1438761-1438780 MS.gene86383:CDS 35.0%
TTAGTGAGCTTCTAACCAAT+TGG + chr2.3:1438317-1438336 MS.gene86383:CDS 35.0%
TTGGATTTGGCAGTGAAAAT+TGG + chr2.3:1438166-1438185 MS.gene86383:intron 35.0%
TTTGAACTGTACTGTGTAAG+GGG - chr2.3:1438474-1438493 None:intergenic 35.0%
! TCTCATCATTGGCCTAAATT+TGG + chr2.3:1438737-1438756 MS.gene86383:CDS 35.0%
! TTACGTTTTGCATCATATCC+TGG - chr2.3:1438888-1438907 None:intergenic 35.0%
! TTCCTATCAGGATTTTTCCT+TGG + chr2.3:1438807-1438826 MS.gene86383:CDS 35.0%
!! ATTTTAATGCGCCCTCAATT+TGG + chr2.3:1438837-1438856 MS.gene86383:CDS 35.0%
!! CTATATTTGCTTTCTGGCTT+TGG + chr2.3:1438193-1438212 MS.gene86383:CDS 35.0%
!!! ACATTTTAAAACCCCATGCA+TGG - chr2.3:1437588-1437607 None:intergenic 35.0%
!!! ATGCATGGGGTTTTAAAATG+TGG + chr2.3:1437587-1437606 MS.gene86383:intron 35.0%
AACACAATGGTTCTCATGGT+TGG + chr2.3:1437176-1437195 MS.gene86383:CDS 40.0%
AACCATGAGAACCATTGTGT+TGG - chr2.3:1437177-1437196 None:intergenic 40.0%
ACTTGCATGTTTCTTCATGC+AGG + chr2.3:1437975-1437994 MS.gene86383:CDS 40.0%
AGGCTATCAGTATTTCTTCC+TGG + chr2.3:1438920-1438939 MS.gene86383:CDS 40.0%
ATCTGTTAGAACAACAGCCT+TGG - chr2.3:1438666-1438685 None:intergenic 40.0%
CAGTCATTACGCTACTTATC+GGG + chr2.3:1438949-1438968 MS.gene86383:CDS 40.0%
CATATAGGAGGATTCCTATC+AGG + chr2.3:1438795-1438814 MS.gene86383:CDS 40.0%
CCTACAATACATGCATGCAT+AGG + chr2.3:1437802-1437821 MS.gene86383:intron 40.0%
CCTATGCATGCATGTATTGT+AGG - chr2.3:1437805-1437824 None:intergenic 40.0%
GACAATTCAGCTCATATAGG+AGG + chr2.3:1438783-1438802 MS.gene86383:CDS 40.0%
GCACACTGTAGAAGTAAACA+TGG - chr2.3:1438699-1438718 None:intergenic 40.0%
GGCTTAGCTACATTGTACAT+AGG + chr2.3:1439286-1439305 MS.gene86383:CDS 40.0%
GGTATTAGGAGCTCTTGAAA+AGG + chr2.3:1437914-1437933 MS.gene86383:CDS 40.0%
GTACAATGTAGCTAAGCCAT+AGG - chr2.3:1439284-1439303 None:intergenic 40.0%
GTGGACAATTCAGCTCATAT+AGG + chr2.3:1438780-1438799 MS.gene86383:CDS 40.0%
GTTCGCCTTGAGAATGAATT+TGG + chr2.3:1438044-1438063 MS.gene86383:CDS 40.0%
TGGGTGAAAATGAAGTATGG+CGG + chr2.3:1437946-1437965 MS.gene86383:CDS 40.0%
TGTTTACATTCTGCAGGTTG+AGG + chr2.3:1437892-1437911 MS.gene86383:intron 40.0%
TTCAATGAAAACCCTCTCCT+TGG + chr2.3:1437327-1437346 MS.gene86383:CDS 40.0%
! AGGAGAGGGTTTTCATTGAA+AGG - chr2.3:1437327-1437346 None:intergenic 40.0%
! ATTGGCCTAAATTTGGCTGT+TGG + chr2.3:1438744-1438763 MS.gene86383:CDS 40.0%
! GTTTACATTCTGCAGGTTGA+GGG + chr2.3:1437893-1437912 MS.gene86383:intron 40.0%
! TGGTGGGTGAAAATGAAGTA+TGG + chr2.3:1437943-1437962 MS.gene86383:CDS 40.0%
!! TATTTGCTTTCTGGCTTTGG+TGG + chr2.3:1438196-1438215 MS.gene86383:CDS 40.0%
AGCTCTTGAAAAGGACCTTG+TGG + chr2.3:1437923-1437942 MS.gene86383:CDS 45.0%
ATAACGAGCCGCCTCAATAT+CGG - chr2.3:1437118-1437137 None:intergenic 45.0%
CATGAGAACCATTGTGTTGG+CGG - chr2.3:1437174-1437193 None:intergenic 45.0%
CCTGGATTACTTCCATGCAT+GGG + chr2.3:1437573-1437592 MS.gene86383:intron 45.0%
CTGGATTACTTCCATGCATG+GGG + chr2.3:1437574-1437593 MS.gene86383:intron 45.0%
CTTGAAAAGGACCTTGTGGT+GGG + chr2.3:1437927-1437946 MS.gene86383:CDS 45.0%
GCACCAAATACCGTATCAGT+TGG + chr2.3:1438256-1438275 MS.gene86383:CDS 45.0%
GCACCAACTGATACGGTATT+TGG - chr2.3:1438262-1438281 None:intergenic 45.0%
GGTTAGAAGCTCACTAAGCA+TGG - chr2.3:1438314-1438333 None:intergenic 45.0%
TCCTGGATTACTTCCATGCA+TGG + chr2.3:1437572-1437591 MS.gene86383:intron 45.0%
TCTTGAAAAGGACCTTGTGG+TGG + chr2.3:1437926-1437945 MS.gene86383:CDS 45.0%
TGAGCTGAATTGTCCACATG+AGG - chr2.3:1438777-1438796 None:intergenic 45.0%
! CGTTTTGCATCATATCCTGG+TGG - chr2.3:1438885-1438904 None:intergenic 45.0%
! CTTCATTTTCACCCACCACA+AGG - chr2.3:1437941-1437960 None:intergenic 45.0%
! TACCGTATCAGTTGGTGCAT+CGG + chr2.3:1438264-1438283 MS.gene86383:CDS 45.0%
! TCACGGAACACTGTCCTTTT+TGG - chr2.3:1438429-1438448 None:intergenic 45.0%
!! CTCTGTGTTTTTGACCGTAG+AGG - chr2.3:1438570-1438589 None:intergenic 45.0%
!! GGTGCACTTTTTGGACTTCT+GGG + chr2.3:1438286-1438305 MS.gene86383:CDS 45.0%
!! TTCTGCAGGTTGAGGGTATT+AGG + chr2.3:1437900-1437919 MS.gene86383:intron 45.0%
AAGGACAGTGTTCCGTGACT+CGG + chr2.3:1438431-1438450 MS.gene86383:intron 50.0%
ACCCGATGCACCAACTGATA+CGG - chr2.3:1438269-1438288 None:intergenic 50.0%
AGGGAGAAGATTCTCACGTG+CGG - chr2.3:1437362-1437381 None:intergenic 50.0%
ATGTAGAAAGTAGGCGGTGG+TGG - chr2.3:1437147-1437166 None:intergenic 50.0%
ATTCTCACGTGCGGACAGAA+GGG - chr2.3:1437353-1437372 None:intergenic 50.0%
CCCATGCATGGAAGTAATCC+AGG - chr2.3:1437576-1437595 None:intergenic 50.0%
CGCCAACACAATGGTTCTCA+TGG + chr2.3:1437172-1437191 MS.gene86383:CDS 50.0%
GCGTAATGACTGACAAGACC+AGG - chr2.3:1438941-1438960 None:intergenic 50.0%
GGTGGCATGTAGAAAGTAGG+CGG - chr2.3:1437153-1437172 None:intergenic 50.0%
! ACCGTATCAGTTGGTGCATC+GGG + chr2.3:1438265-1438284 MS.gene86383:CDS 50.0%
!! AAGAGGCGTTGTAGCCTCTA+CGG + chr2.3:1438553-1438572 MS.gene86383:intron 50.0%
!! GGGTGCACTTTTTGGACTTC+TGG + chr2.3:1438285-1438304 MS.gene86383:CDS 50.0%
ACATGCCACCGCCAACACAA+TGG + chr2.3:1437163-1437182 MS.gene86383:CDS 55.0%
AGGACAGTGTTCCGTGACTC+GGG + chr2.3:1438432-1438451 MS.gene86383:intron 55.0%
AGGGTGGGTGTGTGCCAAAA+AGG + chr2.3:1438412-1438431 MS.gene86383:intron 55.0%
GATTCTCACGTGCGGACAGA+AGG - chr2.3:1437354-1437373 None:intergenic 55.0%
GCCGCCTCAATATCGGAGAA+CGG - chr2.3:1437111-1437130 None:intergenic 55.0%
GCCGTTCTCCGATATTGAGG+CGG + chr2.3:1437107-1437126 MS.gene86383:CDS 55.0%
GGCATGTAGAAAGTAGGCGG+TGG - chr2.3:1437150-1437169 None:intergenic 55.0%
GGCGGTGGCATGTAGAAAGT+AGG - chr2.3:1437156-1437175 None:intergenic 55.0%
GTCGCCGTTCTCCGATATTG+AGG + chr2.3:1437104-1437123 MS.gene86383:CDS 55.0%
! GAGAACCATTGTGTTGGCGG+TGG - chr2.3:1437171-1437190 None:intergenic 55.0%
! GGTGCATCGGGTGCACTTTT+TGG + chr2.3:1438277-1438296 MS.gene86383:CDS 55.0%
! TAGAAAGTAGGCGGTGGTGG+CGG - chr2.3:1437144-1437163 None:intergenic 55.0%
GGACAGAAGGGCCAAGGAGA+GGG - chr2.3:1437341-1437360 None:intergenic 60.0%
GGACAGTGTTCCGTGACTCG+GGG + chr2.3:1438433-1438452 MS.gene86383:intron 60.0%
! AGAAAGTAGGCGGTGGTGGC+GGG - chr2.3:1437143-1437162 None:intergenic 60.0%
ACGTGCGGACAGAAGGGCCA+AGG - chr2.3:1437347-1437366 None:intergenic 65.0%
CGGACAGAAGGGCCAAGGAG+AGG - chr2.3:1437342-1437361 None:intergenic 65.0%
! GAAAGTAGGCGGTGGTGGCG+GGG - chr2.3:1437142-1437161 None:intergenic 65.0%
! GTTCCGTGACTCGGGGTGCA+GGG + chr2.3:1438440-1438459 MS.gene86383:intron 65.0%
! TGTTCCGTGACTCGGGGTGC+AGG + chr2.3:1438439-1438458 MS.gene86383:intron 65.0%
! TTCCGTGACTCGGGGTGCAG+GGG + chr2.3:1438441-1438460 MS.gene86383:intron 65.0%
!! AAAGTAGGCGGTGGTGGCGG+GGG - chr2.3:1437141-1437160 None:intergenic 65.0%
! TCCGTGACTCGGGGTGCAGG+GGG + chr2.3:1438442-1438461 MS.gene86383:intron 70.0%
CCCCCCTGCACCCCGAGTCA+CGG - chr2.3:1438446-1438465 None:intergenic 75.0%
! CCGTGACTCGGGGTGCAGGG+GGG + chr2.3:1438443-1438462 MS.gene86383:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 1437093 1439354 1437093 ID=MS.gene86383
chr2.3 mRNA 1437093 1439354 1437093 ID=MS.gene86383.t1;Parent=MS.gene86383
chr2.3 exon 1437093 1437364 1437093 ID=MS.gene86383.t1.exon1;Parent=MS.gene86383.t1
chr2.3 CDS 1437093 1437364 1437093 ID=cds.MS.gene86383.t1;Parent=MS.gene86383.t1
chr2.3 exon 1437908 1438071 1437908 ID=MS.gene86383.t1.exon2;Parent=MS.gene86383.t1
chr2.3 CDS 1437908 1438071 1437908 ID=cds.MS.gene86383.t1;Parent=MS.gene86383.t1
chr2.3 exon 1438179 1438357 1438179 ID=MS.gene86383.t1.exon3;Parent=MS.gene86383.t1
chr2.3 CDS 1438179 1438357 1438179 ID=cds.MS.gene86383.t1;Parent=MS.gene86383.t1
chr2.3 exon 1438714 1438970 1438714 ID=MS.gene86383.t1.exon4;Parent=MS.gene86383.t1
chr2.3 CDS 1438714 1438970 1438714 ID=cds.MS.gene86383.t1;Parent=MS.gene86383.t1
chr2.3 exon 1439276 1439354 1439276 ID=MS.gene86383.t1.exon5;Parent=MS.gene86383.t1
chr2.3 CDS 1439276 1439354 1439276 ID=cds.MS.gene86383.t1;Parent=MS.gene86383.t1
Gene Sequence

>MS.gene86383

ATGGGGAAAAAGTCGCCGTTCTCCGATATTGAGGCGGCTCGTTATCCCCCGCCACCACCGCCTACTTTCTACATGCCACCGCCAACACAATGGTTCTCATGGTTGGTGCCACTCATTTTTTTAGCCAATATTGCAATGTTCGTGTATAGCATGTATATCAATGATTGCCCTGGCTATTTGAATGAAGATGATTGCCTTTGGTATCAATATTTGGGAAGATTTTCTTTTCAACCTTTCAATGAAAACCCTCTCCTTGGCCCTTCTGTCCGCACGTGAGAATCTTCTCCCTCTCAATTTCTCTCTCACATAATCATCATCATCATTTTAAATATCATGGTATAATGTTCAACAATGTACCTGGCTTTCTCATTAAAATATCATGGTATATTGCATTTTCATATTGAAAAGTTAAGATTTTTGAAATGATGAACATTTAGAATTTTTGAATCATCAATTTGAATGATTATGTCAAATGCATGTCCTGGATTACTTCCATGCATGGGGTTTTAAAATGTGGTTTGCAACAGCAATTGCAGCCGCAATATAACAAAAAATTGAGGTCTCTGCAATGACGTCGCAGCTACAATATCAACTGAATCGACCGCATTTTGCCGAAATATAAAAAATTGCGGTGCGCAACAGCAATTTAAAACTATGCTTCTATTGTCTTACTTAGATGGTATTCAACATAAGACATAAAATGAAAATGCCTACAATACATGCATGCATAGGAAGAAGACGAAAAAAAACAGTGTTAAGTTTCATCCTCTTTTGTCAATTGGTTGTTTTAGGGTTATTGTGTTTACATTCTGCAGGTTGAGGGTATTAGGAGCTCTTGAAAAGGACCTTGTGGTGGGTGAAAATGAAGTATGGCGGTTCTTTACTTGCATGTTTCTTCATGCAGGAGTTATACATTTACTTGCCAATATGTTTAGTCTTTTATTCATAGGAGTTCGCCTTGAGAATGAATTTGGATTTTGTAAGCATATCTCAATTTCTATTGAGTTTTTTTTTTCAACAAGTTGAAACTCTTTTCTATCTTTGTTTAGTTGATTTACTTATTATATTTATTCTTGGATTTGGCAGTGAAAATTGGAGTTCTATATTTGCTTTCTGGCTTTGGTGGAAGTTTGTTATCTATTTTGCATTTGGGAGATGCTAAAGCACCAAATACCGTATCAGTTGGTGCATCGGGTGCACTTTTTGGACTTCTGGGAGCCATGCTTAGTGAGCTTCTAACCAATTGGACTATCTATTTAAATAAGGTACTTTATGATGATATATTTTGTTGTCTTGAAAGAAAGAAAAAAAAAATACATAGGGTGGGTGTGTGCCAAAAAGGACAGTGTTCCGTGACTCGGGGTGCAGGGGGGAGAAACCCCTTACACAGTACAGTTCAAAGTTAATCTTGTAAATTGGACTGTAGCATTTAAGTACTGTAAACCATAAGCTTGATTATAAAGAGGCGTTGTAGCCTCTACGGTCAAAAACACAGAGACAATTTGGTCATATCAAGTGATCAAATATGTGTTTTCTCTACTTGCTTCTTTTGTATCTTTTTACTTTTGAACCAAGGCTGTTGTTCTAACAGATAGATTTGAAACCATGTTTACTTCTACAGTGTGCAGCTCTAACAAGTCTACTTCTCATCATTGGCCTAAATTTGGCTGTTGGATTTATACCTCATGTGGACAATTCAGCTCATATAGGAGGATTCCTATCAGGATTTTTCCTTGGTTTTGTTATTTTAATGCGCCCTCAATTTGGTTATGTGAACAACAAATATATACCACCAGGATATGATGCAAAACGTAAATCCAAGTATAAAGGCTATCAGTATTTCTTCCTGGTCTTGTCAGTCATTACGCTACTTATCGGGTAAGTAATTCATGATTTTCTTTGAGTTTAATTTCGATACAGTGTTGTCAATGTAAACAAATTTACACCGCCAAACAAATCACTATCATTTCATGTGTGTGACTTTTTGAATACTTATGATTAAAGTCAAACGTTTTTAATAATTTGAGGACTTTGATTGATTGGCAATGTAAAAAGTATCAACAGTGACAATGTATATAAATTTAATCATTTCTTTTTTTATGTTCAATTATAATGATGGAATTTGAATTTAATCTGTATTGGTTAACCTCCTTTAACTTTTGATGTAATTGCTCATTTGACAGATATGCCTATGGCTTAGCTACATTGTACATAGGAGAATCAAATGATGTATTTTCCTTCTTAGCGAATACTGAAAGTTAA
Protein sequence

>MS.gene86383.t1

MGKKSPFSDIEAARYPPPPPPTFYMPPPTQWFSWLVPLIFLANIAMFVYSMYINDCPGYLNEDDCLWYQYLGRFSFQPFNENPLLGPSVRTLRVLGALEKDLVVGENEVWRFFTCMFLHAGVIHLLANMFSLLFIGVRLENEFGFLKIGVLYLLSGFGGSLLSILHLGDAKAPNTVSVGASGALFGLLGAMLSELLTNWTIYLNKCAALTSLLLIIGLNLAVGFIPHVDNSAHIGGFLSGFFLGFVILMRPQFGYVNNKYIPPGYDAKRKSKYKGYQYFFLVLSVITLLIGYAYGLATLYIGESNDVFSFLANTES