AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86677

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86677.t1 MTR_7g027175 98.627 437 6 0 1 437 1 437 0.0 894
MS.gene86677.t1 MTR_3g098980 66.587 419 137 2 20 437 18 434 0.0 585
MS.gene86677.t1 MTR_8g096530 61.449 428 160 3 12 437 4 428 0.0 538
MS.gene86677.t1 MTR_4g007540 34.483 435 266 7 13 435 3 430 2.87e-84 265
MS.gene86677.t1 MTR_2g105330 34.475 438 261 9 13 435 3 429 4.96e-83 262
MS.gene86677.t1 MTR_2g064495 34.247 438 262 9 13 435 3 429 5.03e-82 259
MS.gene86677.t1 MTR_8g075610 34.597 422 262 8 23 435 1 417 9.46e-79 250
MS.gene86677.t1 MTR_5g076700 35.917 387 234 8 20 397 14 395 6.75e-77 247
MS.gene86677.t1 MTR_1g053315 36.456 395 229 14 36 412 41 431 9.45e-76 244
MS.gene86677.t1 MTR_1g081660 34.419 430 255 11 19 431 21 440 6.43e-75 241
MS.gene86677.t1 MTR_7g114520 32.870 432 264 10 20 433 15 438 2.06e-72 235
MS.gene86677.t1 MTR_7g114470 34.367 387 239 8 19 395 14 395 2.63e-72 235
MS.gene86677.t1 MTR_1g040175 33.333 423 264 10 20 429 13 430 1.46e-71 233
MS.gene86677.t1 MTR_1g040215 33.596 381 241 8 23 396 16 391 1.09e-70 231
MS.gene86677.t1 MTR_7g114550 32.637 383 247 5 20 395 14 392 5.63e-68 223
MS.gene86677.t1 MTR_7g084940 30.353 425 279 7 22 435 22 440 1.86e-67 222
MS.gene86677.t1 MTR_7g114490 33.073 384 246 6 19 395 13 392 2.05e-67 223
MS.gene86677.t1 MTR_7g114560 31.854 383 250 6 20 395 14 392 1.24e-65 217
MS.gene86677.t1 MTR_1g040195 33.420 383 224 8 19 397 12 367 1.93e-65 216
MS.gene86677.t1 MTR_3g112180 32.947 431 258 13 22 436 18 433 8.48e-65 215
MS.gene86677.t1 MTR_3g026950 30.769 442 266 12 24 432 10 444 1.44e-63 212
MS.gene86677.t1 MTR_3g049150 31.350 437 272 10 24 435 10 443 6.33e-63 210
MS.gene86677.t1 MTR_1g040320 30.024 423 242 10 20 429 13 394 5.62e-61 204
MS.gene86677.t1 MTR_4g055170 32.366 448 266 15 18 436 14 453 6.97e-61 205
MS.gene86677.t1 MTR_7g056087 30.626 431 281 8 20 435 8 435 1.68e-60 203
MS.gene86677.t1 MTR_7g084910 30.695 417 256 11 22 426 23 418 4.84e-60 202
MS.gene86677.t1 MTR_4g127650 32.506 403 235 9 24 396 18 413 3.35e-58 197
MS.gene86677.t1 MTR_1g040315 32.021 381 212 10 21 396 14 352 2.18e-55 189
MS.gene86677.t1 MTR_3g071320 31.422 436 269 12 20 436 9 433 5.76e-55 189
MS.gene86677.t1 MTR_4g097090 29.777 403 244 9 24 395 17 411 2.51e-48 171
MS.gene86677.t1 MTR_7g086000 30.310 419 240 16 13 391 2 408 3.07e-46 166
MS.gene86677.t1 MTR_3g465990 31.421 401 228 17 23 392 17 401 7.39e-45 162
MS.gene86677.t1 MTR_2g015430 29.056 413 274 11 39 437 29 436 1.72e-44 161
MS.gene86677.t1 MTR_3g465930 32.500 400 229 15 23 392 17 405 2.09e-44 161
MS.gene86677.t1 MTR_3g465980 31.920 401 226 19 23 392 17 401 2.66e-43 158
MS.gene86677.t1 MTR_3g465910 32.250 400 227 15 23 392 17 402 3.41e-43 159
MS.gene86677.t1 MTR_6g053810 31.635 373 229 12 38 392 42 406 1.03e-42 156
MS.gene86677.t1 MTR_0502s0010 36.250 240 141 6 104 338 1 233 1.55e-42 150
MS.gene86677.t1 MTR_7g114510 30.169 295 192 6 149 433 6 296 1.05e-40 147
MS.gene86677.t1 MTR_4g013050 24.887 442 274 11 14 435 3 406 5.95e-40 148
MS.gene86677.t1 MTR_7g085990 28.906 384 231 10 51 396 37 416 1.04e-39 148
MS.gene86677.t1 MTR_3g465950 34.416 308 184 11 65 360 65 366 5.62e-39 146
MS.gene86677.t1 MTR_7g085930 28.894 398 213 12 51 396 41 420 7.93e-39 146
MS.gene86677.t1 MTR_3g465880 28.222 450 283 16 23 436 17 462 3.85e-38 144
MS.gene86677.t1 MTR_7g110610 29.534 386 204 14 23 398 12 339 3.66e-35 134
MS.gene86677.t1 MTR_3g086020 26.866 402 247 15 23 408 12 382 2.42e-34 132
MS.gene86677.t1 MTR_5g090810 27.877 391 240 13 37 393 31 413 7.71e-33 129
MS.gene86677.t1 MTR_0042s0190 31.034 232 149 7 169 395 1 226 5.83e-32 123
MS.gene86677.t1 MTR_5g090830 27.621 391 241 13 37 393 31 413 6.58e-32 127
MS.gene86677.t1 MTR_3g101960 29.841 315 195 10 56 356 56 358 3.48e-29 119
MS.gene86677.t1 MTR_3g101930 26.847 406 252 16 55 432 57 445 3.25e-27 113
MS.gene86677.t1 MTR_4g094828 26.733 404 249 17 58 429 49 437 9.72e-27 112
MS.gene86677.t1 MTR_5g090880 25.118 422 269 15 9 393 4 415 2.28e-26 111
MS.gene86677.t1 MTR_0190s0020 25.450 389 259 10 63 433 58 433 7.22e-26 109
MS.gene86677.t1 MTR_3g102020 28.338 367 227 13 14 356 5 359 1.10e-25 108
MS.gene86677.t1 MTR_4g094842 26.485 404 255 14 54 429 46 435 1.34e-25 108
MS.gene86677.t1 MTR_3g101900 25.248 404 258 13 55 432 53 438 1.54e-25 108
MS.gene86677.t1 MTR_3g465870 31.310 313 189 12 9 302 2 307 2.60e-25 107
MS.gene86677.t1 MTR_3g101970 29.355 310 189 11 56 356 57 345 3.50e-25 107
MS.gene86677.t1 MTR_3g088420 25.897 390 266 12 63 437 50 431 5.08e-25 107
MS.gene86677.t1 MTR_3g049140 32.212 208 125 3 244 435 33 240 8.88e-25 102
MS.gene86677.t1 MTR_7g114420 30.638 235 125 7 169 397 1 203 1.36e-24 102
MS.gene86677.t1 MTR_3g450710 25.455 385 240 12 45 394 41 413 2.10e-24 105
MS.gene86677.t1 MTR_4g094835 25.823 395 249 16 61 429 57 433 3.33e-24 104
MS.gene86677.t1 MTR_3g450690 25.915 355 226 11 68 394 72 417 4.02e-24 104
MS.gene86677.t1 MTR_4g022670 26.873 387 245 13 68 429 55 428 4.12e-24 104
MS.gene86677.t1 MTR_4g022800 25.897 390 256 14 63 429 50 429 4.19e-24 104
MS.gene86677.t1 MTR_4g022720 25.501 349 230 11 63 392 50 387 6.77e-24 103
MS.gene86677.t1 MTR_7g114500 35.971 139 86 2 20 155 14 152 1.16e-23 97.1
MS.gene86677.t1 MTR_4g094838 25.562 356 211 11 61 392 57 382 2.87e-23 101
MS.gene86677.t1 MTR_0042s0230 40.441 136 78 2 19 151 12 147 3.30e-23 95.9
MS.gene86677.t1 MTR_8g079710 26.551 403 246 19 58 429 49 432 1.09e-22 100
MS.gene86677.t1 MTR_4g022290 25.628 398 255 14 58 429 50 432 1.95e-22 99.4
MS.gene86677.t1 MTR_1g094790 24.368 435 277 15 13 413 1 417 2.53e-22 99.4
MS.gene86677.t1 MTR_5g026320 24.876 402 260 15 37 415 41 423 3.67e-22 99.0
MS.gene86677.t1 MTR_3g051790 37.705 122 73 2 20 138 17 138 3.93e-22 93.6
MS.gene86677.t1 MTR_8g041770 26.393 341 232 10 63 392 59 391 4.19e-22 98.6
MS.gene86677.t1 MTR_6g045553 26.625 477 284 22 13 437 12 474 6.34e-22 98.2
MS.gene86677.t1 MTR_7g114960 25.824 364 241 11 12 362 5 352 6.59e-22 98.2
MS.gene86677.t1 MTR_4g094832 26.934 349 222 14 98 429 85 417 2.29e-21 96.3
MS.gene86677.t1 MTR_8g079970 25.122 410 249 16 13 392 1 382 4.14e-21 95.5
MS.gene86677.t1 MTR_7g026860 24.479 384 265 12 63 429 50 425 6.04e-21 95.1
MS.gene86677.t1 MTR_4g022760 28.205 273 178 9 68 327 55 322 7.52e-21 94.4
MS.gene86677.t1 MTR_3g070170 24.523 367 222 11 68 392 66 419 9.35e-21 94.7
MS.gene86677.t1 MTR_8g467590 23.469 392 264 13 20 392 12 386 1.36e-20 94.0
MS.gene86677.t1 MTR_5g081960 23.316 386 228 11 67 393 60 436 1.47e-20 94.4
MS.gene86677.t1 MTR_0042s0190 30.303 165 108 4 234 395 1 161 2.71e-20 89.7
MS.gene86677.t1 MTR_4g022825 26.154 390 243 17 68 429 55 427 4.65e-20 92.4
MS.gene86677.t1 MTR_8g079950 22.667 450 289 16 13 432 1 421 6.69e-20 92.0
MS.gene86677.t1 MTR_5g084870 21.995 441 297 14 13 432 1 415 3.27e-19 89.7
MS.gene86677.t1 MTR_8g467580 23.833 407 258 13 15 392 3 386 1.12e-18 88.2
MS.gene86677.t1 MTR_7g014330 24.148 352 235 10 68 391 62 409 1.86e-18 87.8
MS.gene86677.t1 MTR_3g102010 24.619 394 255 14 25 390 19 398 3.98e-18 86.7
MS.gene86677.t1 MTR_7g014160 24.155 414 257 16 67 435 68 469 6.56e-18 86.3
MS.gene86677.t1 MTR_7g014200 23.833 407 260 14 68 435 69 464 1.22e-17 85.1
MS.gene86677.t1 MTR_2g089735 22.778 360 238 11 68 392 62 416 4.53e-17 83.6
MS.gene86677.t1 MTR_7g014230 24.716 352 233 10 68 391 64 411 8.15e-17 82.8
MS.gene86677.t1 MTR_2g089650 24.930 357 233 10 68 392 65 418 1.30e-16 82.0
MS.gene86677.t1 MTR_5g084900 23.421 380 226 13 68 432 9 338 1.44e-16 81.3
MS.gene86677.t1 MTR_4g007460 31.159 138 82 3 82 217 1 127 2.44e-16 76.3
MS.gene86677.t1 MTR_7g014250 23.729 354 234 11 68 391 62 409 2.50e-16 81.3
MS.gene86677.t1 MTR_3g035000 25.758 198 116 6 202 397 43 211 1.89e-15 77.0
MS.gene86677.t1 MTR_7g014610 22.837 416 263 15 67 435 67 471 1.71e-14 75.5
MS.gene86677.t1 MTR_8g039950 34.906 106 66 2 21 123 14 119 2.54e-14 70.1
MS.gene86677.t1 MTR_6g015815 24.286 350 232 12 68 391 70 412 2.85e-14 75.1
MS.gene86677.t1 MTR_1g011660 23.457 324 225 10 48 359 39 351 3.62e-13 71.2
MS.gene86677.t1 MTR_6g015320 23.134 268 172 8 150 391 147 406 4.17e-13 71.2
MS.gene86677.t1 MTR_2g089670 21.289 357 241 9 67 391 81 429 7.97e-13 70.5
MS.gene86677.t1 MTR_6g016815 25.941 239 150 9 63 290 64 286 3.38e-12 68.2
MS.gene86677.t1 MTR_7g085960 27.451 153 77 3 261 396 11 146 7.97e-12 64.7
MS.gene86677.t1 MTR_2g045550 28.947 152 97 5 15 165 4 145 8.09e-12 63.2
MS.gene86677.t1 MTR_3g100150 23.629 237 160 7 48 275 46 270 9.37e-12 67.0
MS.gene86677.t1 MTR_8g012750 22.520 373 242 11 67 397 61 428 1.03e-11 67.0
MS.gene86677.t1 MTR_3g049080 36.986 73 44 1 24 94 10 82 4.06e-11 59.3
MS.gene86677.t1 MTR_7g013850 21.163 430 270 17 62 436 62 477 9.62e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86677.t1 AT5G41040 73.210 433 110 4 11 437 25 457 0.0 671
MS.gene86677.t1 AT5G41040 73.210 433 110 4 11 437 9 441 0.0 669
MS.gene86677.t1 AT5G63560 61.283 421 153 4 20 437 13 426 0.0 526
MS.gene86677.t1 AT3G48720 55.609 419 182 3 21 437 14 430 2.63e-177 502
MS.gene86677.t1 AT5G48930 34.174 436 268 7 12 435 2 430 1.71e-81 258
MS.gene86677.t1 AT1G65450 36.028 433 253 12 20 436 22 446 5.02e-77 247
MS.gene86677.t1 AT1G03390 35.747 442 264 14 13 437 19 457 7.34e-77 247
MS.gene86677.t1 AT1G27620 33.724 427 260 9 19 435 12 425 1.41e-73 238
MS.gene86677.t1 AT5G17540 32.637 383 247 7 20 395 13 391 5.74e-64 213
MS.gene86677.t1 AT3G03480 34.987 383 231 10 23 395 26 400 1.98e-62 209
MS.gene86677.t1 AT1G28680 32.258 434 270 12 20 435 13 440 6.64e-57 194
MS.gene86677.t1 AT2G19070 32.000 400 238 11 26 396 14 408 1.27e-56 193
MS.gene86677.t1 AT5G57840 32.316 393 235 8 20 390 7 390 5.45e-56 191
MS.gene86677.t1 AT2G40230 30.787 432 271 11 20 435 9 428 1.72e-53 185
MS.gene86677.t1 AT1G28680 32.565 347 222 8 20 360 13 353 4.25e-50 175
MS.gene86677.t1 AT4G31910 30.189 424 259 13 31 423 26 443 5.95e-48 171
MS.gene86677.t1 AT5G07080 30.227 440 269 17 19 433 17 443 5.52e-45 162
MS.gene86677.t1 AT1G65450 32.639 288 176 7 161 436 1 282 6.79e-45 158
MS.gene86677.t1 AT2G25150 30.995 442 264 14 23 435 20 449 3.00e-41 152
MS.gene86677.t1 AT3G62160 29.592 392 238 12 22 398 11 379 6.13e-41 151
MS.gene86677.t1 AT3G47170 28.795 448 284 13 19 433 15 460 6.34e-41 152
MS.gene86677.t1 AT2G23510 28.894 443 272 16 23 436 20 448 4.52e-37 141
MS.gene86677.t1 AT1G78990 27.047 403 245 14 48 414 46 435 1.71e-33 131
MS.gene86677.t1 AT1G24430 27.818 417 254 17 35 432 27 415 8.02e-31 123
MS.gene86677.t1 AT1G31490 27.621 391 249 15 37 410 29 402 3.57e-29 119
MS.gene86677.t1 AT1G32910 24.796 367 247 11 46 393 44 400 9.22e-29 118
MS.gene86677.t1 AT5G16410 26.562 384 244 14 37 400 61 426 8.19e-27 112
MS.gene86677.t1 AT5G07870 28.754 313 196 9 62 356 66 369 1.69e-26 111
MS.gene86677.t1 AT5G42830 28.902 346 218 10 29 356 22 357 3.04e-26 110
MS.gene86677.t1 AT2G39980 26.455 378 215 11 68 392 62 429 5.87e-26 110
MS.gene86677.t1 AT5G23940 27.586 406 258 14 51 429 43 439 9.02e-24 103
MS.gene86677.t1 AT3G29670 24.112 394 271 12 48 419 42 429 1.24e-23 103
MS.gene86677.t1 AT5G07860 27.508 309 195 8 62 356 66 359 1.84e-23 102
MS.gene86677.t1 AT3G50270 26.463 393 250 17 68 434 62 441 4.16e-23 101
MS.gene86677.t1 AT5G61160 25.352 355 234 12 68 399 64 410 7.71e-23 100
MS.gene86677.t1 AT4G15390 23.624 436 286 16 23 432 15 429 1.33e-22 100
MS.gene86677.t1 AT4G15390 23.624 436 286 16 23 432 15 429 1.33e-22 100
MS.gene86677.t1 AT3G29635 24.289 387 267 11 71 436 70 451 1.41e-22 100
MS.gene86677.t1 AT1G24420 24.658 365 243 10 79 432 76 419 3.31e-22 99.0
MS.gene86677.t1 AT1G24420 24.658 365 243 10 79 432 76 419 3.31e-22 99.0
MS.gene86677.t1 AT3G30280 25.000 428 273 20 23 424 13 418 7.76e-21 94.7
MS.gene86677.t1 AT5G47950 24.439 401 254 17 48 432 42 409 5.10e-20 92.0
MS.gene86677.t1 AT3G50280 22.938 388 268 14 68 434 59 436 3.68e-19 89.7
MS.gene86677.t1 AT5G38130 24.251 367 250 12 71 419 82 438 2.51e-18 87.4
MS.gene86677.t1 AT5G23970 23.472 409 259 12 37 421 24 402 3.59e-18 86.7
MS.gene86677.t1 AT5G47980 23.744 438 281 18 23 431 12 425 5.94e-18 86.3
MS.gene86677.t1 AT3G29590 25.220 341 223 13 76 392 69 401 7.41e-18 85.9
MS.gene86677.t1 AT1G65450 45.455 110 55 4 20 125 22 130 1.41e-17 80.5
MS.gene86677.t1 AT4G15400 24.356 427 288 17 23 433 12 419 3.06e-17 84.0
MS.gene86677.t1 AT5G07850 25.364 343 216 14 20 343 27 348 2.21e-16 81.3
MS.gene86677.t1 AT5G67150 22.973 370 238 13 68 407 61 413 2.68e-16 81.3
MS.gene86677.t1 AT3G29680 22.481 387 276 8 68 435 62 443 3.53e-16 80.9
MS.gene86677.t1 AT3G50300 24.064 374 250 13 68 419 59 420 1.24e-15 79.0
MS.gene86677.t1 AT5G39090 22.667 375 269 11 78 436 75 444 3.72e-14 74.7
MS.gene86677.t1 AT1G03495 27.778 288 168 15 68 322 66 346 1.34e-13 72.0
MS.gene86677.t1 AT4G29250 22.844 429 283 17 34 433 33 442 4.65e-13 71.2
MS.gene86677.t1 AT3G50280 22.295 305 217 10 142 434 10 306 6.62e-13 69.7
MS.gene86677.t1 AT1G03940 24.510 408 257 17 68 436 66 461 6.14e-12 67.8
MS.gene86677.t1 AT3G50290 24.906 265 183 7 137 389 9 269 2.35e-11 64.7
MS.gene86677.t1 AT5G39050 21.725 313 219 6 150 436 152 464 6.12e-11 64.7
MS.gene86677.t1 AT4G13840 25.000 224 142 8 68 280 67 275 6.61e-11 64.3

Find 92 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTCCCAAAGGGTATTT+TGG 0.104046 7.4:+74225898 MS.gene86677:CDS
GTCTGGACCGGTTTCCTTGC+CGG 0.230469 7.4:+74226164 MS.gene86677:CDS
CTTCAATGTCAGCAAATTCT+TGG 0.238280 7.4:-74225627 None:intergenic
AGAAGCTTTGTTTCTTGTTC+TGG 0.240151 7.4:-74225841 None:intergenic
GAAAATTCTAATTATCAAAA+TGG 0.257291 7.4:+74224076 MS.gene86677:CDS
CACCATTTCATCTTCATATA+AGG 0.260329 7.4:-74225668 None:intergenic
CCAAGATTTAATGCAGATAT+AGG 0.271308 7.4:+74226278 MS.gene86677:CDS
GTTCATTATTACCCTCTTGC+TGG 0.281316 7.4:+74224301 MS.gene86677:CDS
AAATACCCTTTGGGAAGTTT+TGG 0.290579 7.4:-74225895 None:intergenic
CACTGTATGTTTGATGGAAT+TGG 0.294988 7.4:+74225484 MS.gene86677:CDS
CCGTTTCATACCACCGATTT+CGG 0.298332 7.4:+74226123 MS.gene86677:CDS
AATTGTTCAATGGAAGAAAT+TGG 0.299341 7.4:+74224403 MS.gene86677:CDS
CCTTTCTTTGTTTCTTCAGA+TGG 0.304433 7.4:-74224148 None:intergenic
TGAATAACCTTCACCGTATA+CGG 0.313318 7.4:-74225982 None:intergenic
GAACAAGCCCTTTCCGTATA+CGG 0.314033 7.4:+74225969 MS.gene86677:CDS
AACAAGAGCTAGGCCATCTT+TGG 0.319090 7.4:+74226065 MS.gene86677:CDS
CAAGTGTTGGTTCACTTGTT+AGG 0.323483 7.4:-74224120 None:intergenic
TGGTGCAAATGCTCTATCTT+CGG 0.344041 7.4:-74225607 None:intergenic
TCCATTCCCAAAATACCCTT+TGG 0.350876 7.4:-74225905 None:intergenic
AGAAGCATTAATGTGCTTCT+TGG 0.357845 7.4:+74226228 MS.gene86677:CDS
GGTGCAAATGCTCTATCTTC+GGG 0.375653 7.4:-74225606 None:intergenic
CGATTTCGGTTGGGGAGAAC+CGG 0.379270 7.4:+74226137 MS.gene86677:CDS
GAATGCTTGTGAAGTGATTA+AGG 0.383599 7.4:+74224258 MS.gene86677:CDS
CCTATATCTGCATTAAATCT+TGG 0.386363 7.4:-74226278 None:intergenic
TATTCTTCAGATGCCTCCTT+TGG 0.394177 7.4:+74224492 MS.gene86677:CDS
AAGTGCTTTCAGCATTTGTT+TGG 0.399533 7.4:+74225788 MS.gene86677:CDS
TCCTTCTCCGGCAAGGAAAC+CGG 0.400213 7.4:-74226171 None:intergenic
GATGCCTCCTTTGGTTGCTC+AGG 0.401248 7.4:+74224501 MS.gene86677:CDS
TCATACCACCGATTTCGGTT+GGG 0.412037 7.4:+74226128 MS.gene86677:CDS
CGGTTCTCCCCAACCGAAAT+CGG 0.413358 7.4:-74226136 None:intergenic
TGGGGAGAACCGGTTCTGTC+TGG 0.419323 7.4:+74226147 MS.gene86677:CDS
TTCATATAAGGTACTAGTGT+TGG 0.419653 7.4:-74225656 None:intergenic
TACCTTATATGAAGATGAAA+TGG 0.425519 7.4:+74225666 MS.gene86677:CDS
CCATTCCCAAAATACCCTTT+GGG 0.427070 7.4:-74225904 None:intergenic
AACAAAATCACTTCCTTCTC+CGG 0.435908 7.4:-74226183 None:intergenic
CATACCACCGATTTCGGTTG+GGG 0.437822 7.4:+74226129 MS.gene86677:CDS
TCCAAGGACTCTTGGTATGT+TGG 0.438373 7.4:+74224444 MS.gene86677:CDS
TTCATACCACCGATTTCGGT+TGG 0.441418 7.4:+74226127 MS.gene86677:CDS
TTCATTATTACCCTCTTGCT+GGG 0.443863 7.4:+74224302 MS.gene86677:CDS
TTCTTTGTTTCTTCAGATGG+TGG 0.446811 7.4:-74224145 None:intergenic
AGTATGCTTCGATCGAGAAC+AGG 0.458088 7.4:-74225568 None:intergenic
TGAAGAATATGTTTAGCATC+AGG 0.461984 7.4:-74224478 None:intergenic
AAACCTGATCCAAGGACTCT+TGG 0.464374 7.4:+74224436 MS.gene86677:CDS
ATGAATCACTGTATGTTTGA+TGG 0.464465 7.4:+74225478 MS.gene86677:CDS
ACAAATGCTGTTTGTAAAGC+AGG 0.466739 7.4:+74225937 MS.gene86677:CDS
ATACCAAGAGTCCTTGGATC+AGG 0.478213 7.4:-74224439 None:intergenic
ACCAACATACCAAGAGTCCT+TGG 0.479611 7.4:-74224445 None:intergenic
GGCTGAAGCTAATTGTTCAA+TGG 0.480046 7.4:+74224393 MS.gene86677:CDS
TCAGATGGTGGAACAAGTGT+TGG 0.489688 7.4:-74224133 None:intergenic
ACCGGTTTCCTTGCCGGAGA+AGG 0.494266 7.4:+74226170 MS.gene86677:CDS
CTATGGAGTTTGTGAACTCA+TGG 0.496901 7.4:+74225509 MS.gene86677:CDS
AAAACCTCCACATTTGAACT+TGG 0.504811 7.4:-74225440 None:intergenic
CTCAGAGGGTAAACTGATTG+TGG 0.510531 7.4:+74224339 MS.gene86677:CDS
AAGATCTTCATGACAGGAGC+AGG 0.513671 7.4:-74226255 None:intergenic
ACCTTCACCGTATACGGAAA+GGG 0.514785 7.4:-74225976 None:intergenic
TTGAACCAAAACTTCCCAAA+GGG 0.539116 7.4:+74225890 MS.gene86677:CDS
TCCTCTCTTGACCATGTGAA+AGG 0.539507 7.4:-74226206 None:intergenic
GTTTGATGGAATTGGAGCTA+TGG 0.548532 7.4:+74225492 MS.gene86677:CDS
GAAACCGGTCCAGACAGAAC+CGG 0.551440 7.4:-74226156 None:intergenic
CCGAAATCGGTGGTATGAAA+CGG 0.552192 7.4:-74226123 None:intergenic
AAAAGTGTACAAGCCAAAGA+TGG 0.555885 7.4:-74226078 None:intergenic
AGAACCGGTTCTGTCTGGAC+CGG 0.557075 7.4:+74226152 MS.gene86677:CDS
GCCCTTTCCGTATACGGTGA+AGG 0.557341 7.4:+74225975 MS.gene86677:CDS
TACTGATAGTTACATGAGAT+CGG 0.559146 7.4:+74226023 MS.gene86677:CDS
TATTCAAGATGCAATCAAGA+TGG 0.561804 7.4:+74225999 MS.gene86677:CDS
AGAACAGGAGGCACAGAAAG+TGG 0.562779 7.4:-74225553 None:intergenic
CCATCTGAAGAAACAAAGAA+AGG 0.565357 7.4:+74224148 MS.gene86677:CDS
AAACTGATTGTGGATTGTAC+TGG 0.568236 7.4:+74224349 MS.gene86677:CDS
TCTTGGAAGATCTTCATGAC+AGG 0.573252 7.4:-74226261 None:intergenic
TATGGAGTTTGTGAACTCAT+GGG 0.576378 7.4:+74225510 MS.gene86677:CDS
TCCTTTCACATGGTCAAGAG+AGG 0.581268 7.4:+74226205 MS.gene86677:CDS
AGATTAAGTATAAGCTCAGA+GGG 0.587672 7.4:+74224325 MS.gene86677:CDS
CAGCTGACCAAGTTCAAATG+TGG 0.590914 7.4:+74225433 MS.gene86677:intron
TCTCGATCGAAGCATACTAA+AGG 0.593265 7.4:+74225573 MS.gene86677:CDS
AACCTTCACCGTATACGGAA+AGG 0.593644 7.4:-74225977 None:intergenic
GTGCAAATGCTCTATCTTCG+GGG 0.597277 7.4:-74225605 None:intergenic
AATCACTTCCTTCTCCGGCA+AGG 0.599797 7.4:-74226178 None:intergenic
CTGACCAAGTTCAAATGTGG+AGG 0.605830 7.4:+74225436 MS.gene86677:CDS
TTTGAACCAAAACTTCCCAA+AGG 0.608712 7.4:+74225889 MS.gene86677:CDS
ATACTTAATCTCCCAGCAAG+AGG 0.610225 7.4:-74224313 None:intergenic
TGCAAATGCTCTATCTTCGG+GGG 0.613923 7.4:-74225604 None:intergenic
ATATTACAAAACCTGATCCA+AGG 0.621561 7.4:+74224428 MS.gene86677:CDS
CATACCTGAGCAACCAAAGG+AGG 0.626730 7.4:-74224505 None:intergenic
TACTTAATCTCCCAGCAAGA+GGG 0.629161 7.4:-74224312 None:intergenic
AAACATACCTGAGCAACCAA+AGG 0.647608 7.4:-74224508 None:intergenic
ATGGAGTTTGTGAACTCATG+GGG 0.648584 7.4:+74225511 MS.gene86677:CDS
GAGATTAAGTATAAGCTCAG+AGG 0.659227 7.4:+74224324 MS.gene86677:CDS
ATGCTTCGATCGAGAACAGG+AGG 0.660881 7.4:-74225565 None:intergenic
TTCTCCCCAACCGAAATCGG+TGG 0.671335 7.4:-74226133 None:intergenic
ATTGTGGATTGTACTGGAGA+AGG 0.681300 7.4:+74224355 MS.gene86677:CDS
TCATGGGGAGAACTAGCAAG+AGG 0.683206 7.4:+74225526 MS.gene86677:CDS
AAAGCTTTAGAAGATGATGG+CGG 0.739373 7.4:+74225739 MS.gene86677:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAATTATTTAAAAATTTA+GGG - chr7.4:74224742-74224761 None:intergenic 10.0%
!! GATTAAAAAAATTTAGTTAA+GGG + chr7.4:74224853-74224872 MS.gene86677:intron 10.0%
!! TCCAAATTATTTAAAAATTT+AGG - chr7.4:74224743-74224762 None:intergenic 10.0%
!! TGATTAAAAAAATTTAGTTA+AGG + chr7.4:74224852-74224871 MS.gene86677:intron 10.0%
!!! TTTTTAAATAATTTGGAATT+TGG + chr7.4:74224746-74224765 MS.gene86677:intron 10.0%
!! ACTTCTAAATCATTTATAAT+TGG - chr7.4:74224823-74224842 None:intergenic 15.0%
!! CTTCTAAATCATTTATAATT+GGG - chr7.4:74224822-74224841 None:intergenic 15.0%
!! GAAAATTCTAATTATCAAAA+TGG + chr7.4:74224076-74224095 MS.gene86677:CDS 15.0%
!!! AGTTTTTGTCAAAATTATAA+TGG + chr7.4:74224936-74224955 MS.gene86677:intron 15.0%
!!! CCCTAAATTTTTAAATAATT+TGG + chr7.4:74224739-74224758 MS.gene86677:intron 15.0%
!!! CTATTTGAGAAATTTTAAAT+AGG - chr7.4:74225060-74225079 None:intergenic 15.0%
!! AAATGATTCAATGTATTCAT+AGG + chr7.4:74224644-74224663 MS.gene86677:intron 20.0%
!! ATTTATGTATGATGAACATA+TGG + chr7.4:74225220-74225239 MS.gene86677:intron 20.0%
!!! TTTTTGAAATATAGTTGATG+CGG + chr7.4:74225364-74225383 MS.gene86677:intron 20.0%
! AAAAATATTTCGCACATGTT+TGG - chr7.4:74224994-74225013 None:intergenic 25.0%
! AATTGTTCAATGGAAGAAAT+TGG + chr7.4:74224403-74224422 MS.gene86677:CDS 25.0%
! ACTTCAGTGACAAAAAATAA+AGG - chr7.4:74225105-74225124 None:intergenic 25.0%
! CATACATAAATTCCTATGAA+GGG - chr7.4:74225211-74225230 None:intergenic 25.0%
! TACCTTATATGAAGATGAAA+TGG + chr7.4:74225666-74225685 MS.gene86677:CDS 25.0%
! TCATACATAAATTCCTATGA+AGG - chr7.4:74225212-74225231 None:intergenic 25.0%
! TCATAGGTTACTTGTAATTT+GGG + chr7.4:74224715-74224734 MS.gene86677:intron 25.0%
! TGTATCTCAACTAACATAAA+AGG + chr7.4:74225256-74225275 MS.gene86677:intron 25.0%
! TTCATAGGTTACTTGTAATT+TGG + chr7.4:74224714-74224733 MS.gene86677:intron 25.0%
!! TACTTTTGAACTTAGACATT+TGG - chr7.4:74225134-74225153 None:intergenic 25.0%
!!! AAAAAAGCTTTAGAAGATGA+TGG + chr7.4:74225736-74225755 MS.gene86677:CDS 25.0%
!!! CTTCAATTCTTTTAGTTTCT+CGG - chr7.4:74225716-74225735 None:intergenic 25.0%
!!! TTCAATTCTTTTAGTTTCTC+GGG - chr7.4:74225715-74225734 None:intergenic 25.0%
AGATTAAGTATAAGCTCAGA+GGG + chr7.4:74224325-74224344 MS.gene86677:CDS 30.0%
ATATTACAAAACCTGATCCA+AGG + chr7.4:74224428-74224447 MS.gene86677:CDS 30.0%
ATGAATCACTGTATGTTTGA+TGG + chr7.4:74225478-74225497 MS.gene86677:CDS 30.0%
CACCATTTCATCTTCATATA+AGG - chr7.4:74225671-74225690 None:intergenic 30.0%
TACTGATAGTTACATGAGAT+CGG + chr7.4:74226023-74226042 MS.gene86677:CDS 30.0%
TATTCAAGATGCAATCAAGA+TGG + chr7.4:74225999-74226018 MS.gene86677:CDS 30.0%
TGAAGAATATGTTTAGCATC+AGG - chr7.4:74224481-74224500 None:intergenic 30.0%
TGCTTCAAAAATGAAGAGAA+GGG + chr7.4:74224229-74224248 MS.gene86677:CDS 30.0%
TTGCTTCAAAAATGAAGAGA+AGG + chr7.4:74224228-74224247 MS.gene86677:CDS 30.0%
! ATTTTTGCAGTTGGATTTTC+TGG + chr7.4:74224543-74224562 MS.gene86677:intron 30.0%
! TTCATATAAGGTACTAGTGT+TGG - chr7.4:74225659-74225678 None:intergenic 30.0%
!! TTTGTTAGTTACTTGCTATG+AGG + chr7.4:74224567-74224586 MS.gene86677:intron 30.0%
!!! TACTAGTCTATTTTTGCAGT+TGG + chr7.4:74224534-74224553 MS.gene86677:intron 30.0%
!!! TGTTTTGATCAAGGTTTGAT+AGG - chr7.4:74224183-74224202 None:intergenic 30.0%
!!! TTACAGCAATGTTTTGATCA+AGG - chr7.4:74224192-74224211 None:intergenic 30.0%
AAAACCTCCACATTTGAACT+TGG - chr7.4:74225443-74225462 None:intergenic 35.0%
AACAAAATCACTTCCTTCTC+CGG - chr7.4:74226186-74226205 None:intergenic 35.0%
AAGTGCTTTCAGCATTTGTT+TGG + chr7.4:74225788-74225807 MS.gene86677:CDS 35.0%
CACTGTATGTTTGATGGAAT+TGG + chr7.4:74225484-74225503 MS.gene86677:CDS 35.0%
CCATCTGAAGAAACAAAGAA+AGG + chr7.4:74224148-74224167 MS.gene86677:CDS 35.0%
CTTCAATGTCAGCAAATTCT+TGG - chr7.4:74225630-74225649 None:intergenic 35.0%
GAATGCTTGTGAAGTGATTA+AGG + chr7.4:74224258-74224277 MS.gene86677:CDS 35.0%
GAGATTAAGTATAAGCTCAG+AGG + chr7.4:74224324-74224343 MS.gene86677:CDS 35.0%
TATGGAGTTTGTGAACTCAT+GGG + chr7.4:74225510-74225529 MS.gene86677:CDS 35.0%
TCAAGTGTATCTTTGATGAG+TGG + chr7.4:74224882-74224901 MS.gene86677:intron 35.0%
TGAATAACCTTCACCGTATA+CGG - chr7.4:74225985-74226004 None:intergenic 35.0%
TTCATTATTACCCTCTTGCT+GGG + chr7.4:74224302-74224321 MS.gene86677:CDS 35.0%
TTGAACCAAAACTTCCCAAA+GGG + chr7.4:74225890-74225909 MS.gene86677:CDS 35.0%
TTTGAACCAAAACTTCCCAA+AGG + chr7.4:74225889-74225908 MS.gene86677:CDS 35.0%
! AAAAGTGTACAAGCCAAAGA+TGG - chr7.4:74226081-74226100 None:intergenic 35.0%
! AAACTGATTGTGGATTGTAC+TGG + chr7.4:74224349-74224368 MS.gene86677:CDS 35.0%
! AAATGTGGAGGTTTTTCTCT+TGG + chr7.4:74225448-74225467 MS.gene86677:CDS 35.0%
! ACAAATGCTGTTTGTAAAGC+AGG + chr7.4:74225937-74225956 MS.gene86677:CDS 35.0%
! CCTTTCTTTGTTTCTTCAGA+TGG - chr7.4:74224151-74224170 None:intergenic 35.0%
! CGTCGCAATATTTTATATCG+TGG + chr7.4:74225336-74225355 MS.gene86677:intron 35.0%
! GTACACTTTTGATCACAACT+TGG + chr7.4:74226091-74226110 MS.gene86677:CDS 35.0%
! GTGATTTTGTTCCTTTCACA+TGG + chr7.4:74226195-74226214 MS.gene86677:CDS 35.0%
! TTCTTTGTTTCTTCAGATGG+TGG - chr7.4:74224148-74224167 None:intergenic 35.0%
! TTTTGATCACAACTTGGTCT+AGG + chr7.4:74226097-74226116 MS.gene86677:CDS 35.0%
!! AAAGCTTTAGAAGATGATGG+CGG + chr7.4:74225739-74225758 MS.gene86677:CDS 35.0%
!! AAATACCCTTTGGGAAGTTT+TGG - chr7.4:74225898-74225917 None:intergenic 35.0%
!! AGAAGCATTAATGTGCTTCT+TGG + chr7.4:74226228-74226247 MS.gene86677:CDS 35.0%
!! AGAAGCTTTGTTTCTTGTTC+TGG - chr7.4:74225844-74225863 None:intergenic 35.0%
!! ATCGGCTATCGATTATTTTG+AGG + chr7.4:74226041-74226060 MS.gene86677:CDS 35.0%
!!! AAACTTCCCAAAGGGTATTT+TGG + chr7.4:74225898-74225917 MS.gene86677:CDS 35.0%
!!! AACTTCCCAAAGGGTATTTT+GGG + chr7.4:74225899-74225918 MS.gene86677:CDS 35.0%
!!! AAGCTTCTTTTTGCTGTTGA+TGG + chr7.4:74225856-74225875 MS.gene86677:CDS 35.0%
!!! ATTTTGAGGTAACAAGAGCT+AGG + chr7.4:74226055-74226074 MS.gene86677:CDS 35.0%
!!! TCTTTTTGCTGTTGATGGAA+GGG + chr7.4:74225861-74225880 MS.gene86677:CDS 35.0%
!!! TTCTTTTTGCTGTTGATGGA+AGG + chr7.4:74225860-74225879 MS.gene86677:CDS 35.0%
AAACATACCTGAGCAACCAA+AGG - chr7.4:74224511-74224530 None:intergenic 40.0%
ACAGACACTCCTAAGATTTG+AGG - chr7.4:74224692-74224711 None:intergenic 40.0%
ACTGACACAAATATGGACAC+TGG + chr7.4:74224591-74224610 MS.gene86677:intron 40.0%
ATACTTAATCTCCCAGCAAG+AGG - chr7.4:74224316-74224335 None:intergenic 40.0%
ATGGAGTTTGTGAACTCATG+GGG + chr7.4:74225511-74225530 MS.gene86677:CDS 40.0%
CCATTCCCAAAATACCCTTT+GGG - chr7.4:74225907-74225926 None:intergenic 40.0%
CTATGGAGTTTGTGAACTCA+TGG + chr7.4:74225509-74225528 MS.gene86677:CDS 40.0%
GGCTGAAGCTAATTGTTCAA+TGG + chr7.4:74224393-74224412 MS.gene86677:CDS 40.0%
GTTCATTATTACCCTCTTGC+TGG + chr7.4:74224301-74224320 MS.gene86677:CDS 40.0%
GTTTGATGGAATTGGAGCTA+TGG + chr7.4:74225492-74225511 MS.gene86677:CDS 40.0%
TACTTAATCTCCCAGCAAGA+GGG - chr7.4:74224315-74224334 None:intergenic 40.0%
TATTCTTCAGATGCCTCCTT+TGG + chr7.4:74224492-74224511 MS.gene86677:CDS 40.0%
TCATGTTCCAACTGTAGTTG+TGG - chr7.4:74225390-74225409 None:intergenic 40.0%
TCCATTCCCAAAATACCCTT+TGG - chr7.4:74225908-74225927 None:intergenic 40.0%
TCTCGATCGAAGCATACTAA+AGG + chr7.4:74225573-74225592 MS.gene86677:CDS 40.0%
TCTTGGAAGATCTTCATGAC+AGG - chr7.4:74226264-74226283 None:intergenic 40.0%
TGGTGCAAATGCTCTATCTT+CGG - chr7.4:74225610-74225629 None:intergenic 40.0%
! ATTGTGGATTGTACTGGAGA+AGG + chr7.4:74224355-74224374 MS.gene86677:CDS 40.0%
! TGTATTCATAGGTGTCGTGT+CGG + chr7.4:74224655-74224674 MS.gene86677:intron 40.0%
!! ATGAGGCACTGACACAAATA+TGG + chr7.4:74224584-74224603 MS.gene86677:intron 40.0%
!! CAAGTGTTGGTTCACTTGTT+AGG - chr7.4:74224123-74224142 None:intergenic 40.0%
!! TAGGAGTGTCTGTACTTCAT+AGG + chr7.4:74224699-74224718 MS.gene86677:intron 40.0%
!!! AGAAGGTGCTCTTTTTGTTG+AGG + chr7.4:74224372-74224391 MS.gene86677:CDS 40.0%
!!! GCATTTTTCAAGCCCTTCAT+AGG + chr7.4:74225196-74225215 MS.gene86677:intron 40.0%
AAACCTGATCCAAGGACTCT+TGG + chr7.4:74224436-74224455 MS.gene86677:CDS 45.0%
AACAAGAGCTAGGCCATCTT+TGG + chr7.4:74226065-74226084 MS.gene86677:CDS 45.0%
AACCTTCACCGTATACGGAA+AGG - chr7.4:74225980-74225999 None:intergenic 45.0%
AAGATCTTCATGACAGGAGC+AGG - chr7.4:74226258-74226277 None:intergenic 45.0%
ACCAACATACCAAGAGTCCT+TGG - chr7.4:74224448-74224467 None:intergenic 45.0%
ACCTTCACCGTATACGGAAA+GGG - chr7.4:74225979-74225998 None:intergenic 45.0%
AGTATGCTTCGATCGAGAAC+AGG - chr7.4:74225571-74225590 None:intergenic 45.0%
ATACCAAGAGTCCTTGGATC+AGG - chr7.4:74224442-74224461 None:intergenic 45.0%
CAGCTGACCAAGTTCAAATG+TGG + chr7.4:74225433-74225452 MS.gene86677:intron 45.0%
CCGAAATCGGTGGTATGAAA+CGG - chr7.4:74226126-74226145 None:intergenic 45.0%
CTCAGAGGGTAAACTGATTG+TGG + chr7.4:74224339-74224358 MS.gene86677:CDS 45.0%
CTGACCAAGTTCAAATGTGG+AGG + chr7.4:74225436-74225455 MS.gene86677:CDS 45.0%
GAACAAGCCCTTTCCGTATA+CGG + chr7.4:74225969-74225988 MS.gene86677:CDS 45.0%
GGTGCAAATGCTCTATCTTC+GGG - chr7.4:74225609-74225628 None:intergenic 45.0%
GTGCAAATGCTCTATCTTCG+GGG - chr7.4:74225608-74225627 None:intergenic 45.0%
TCCAAGGACTCTTGGTATGT+TGG + chr7.4:74224444-74224463 MS.gene86677:CDS 45.0%
TCCTCTCTTGACCATGTGAA+AGG - chr7.4:74226209-74226228 None:intergenic 45.0%
TCCTTTCACATGGTCAAGAG+AGG + chr7.4:74226205-74226224 MS.gene86677:CDS 45.0%
TGCAAATGCTCTATCTTCGG+GGG - chr7.4:74225607-74225626 None:intergenic 45.0%
! TCCTAAGATTTGAGGTGTCC+CGG - chr7.4:74224684-74224703 None:intergenic 45.0%
!! CCGTTTCATACCACCGATTT+CGG + chr7.4:74226123-74226142 MS.gene86677:CDS 45.0%
!! TCAGATGGTGGAACAAGTGT+TGG - chr7.4:74224136-74224155 None:intergenic 45.0%
!! TCATACCACCGATTTCGGTT+GGG + chr7.4:74226128-74226147 MS.gene86677:CDS 45.0%
!! TTCATACCACCGATTTCGGT+TGG + chr7.4:74226127-74226146 MS.gene86677:CDS 45.0%
!!! CCCAAAGGGTATTTTGGGAA+TGG + chr7.4:74225904-74225923 MS.gene86677:CDS 45.0%
AATCACTTCCTTCTCCGGCA+AGG - chr7.4:74226181-74226200 None:intergenic 50.0%
ATGCTTCGATCGAGAACAGG+AGG - chr7.4:74225568-74225587 None:intergenic 50.0%
CATACCTGAGCAACCAAAGG+AGG - chr7.4:74224508-74224527 None:intergenic 50.0%
TCATGGGGAGAACTAGCAAG+AGG + chr7.4:74225526-74225545 MS.gene86677:CDS 50.0%
TCCGGGACACCTCAAATCTT+AGG + chr7.4:74224680-74224699 MS.gene86677:intron 50.0%
! AGAACAGGAGGCACAGAAAG+TGG - chr7.4:74225556-74225575 None:intergenic 50.0%
!! CATACCACCGATTTCGGTTG+GGG + chr7.4:74226129-74226148 MS.gene86677:CDS 50.0%
CGATTTCGGTTGGGGAGAAC+CGG + chr7.4:74226137-74226156 MS.gene86677:CDS 55.0%
CGGTTCTCCCCAACCGAAAT+CGG - chr7.4:74226139-74226158 None:intergenic 55.0%
GAAACCGGTCCAGACAGAAC+CGG - chr7.4:74226159-74226178 None:intergenic 55.0%
GATGCCTCCTTTGGTTGCTC+AGG + chr7.4:74224501-74224520 MS.gene86677:CDS 55.0%
GATGCGGCCACAACTACAGT+TGG + chr7.4:74225380-74225399 MS.gene86677:intron 55.0%
GCCCTTTCCGTATACGGTGA+AGG + chr7.4:74225975-74225994 MS.gene86677:CDS 55.0%
GCTCTATCTTCGGGGGATTG+CGG - chr7.4:74225600-74225619 None:intergenic 55.0%
TCCTTCTCCGGCAAGGAAAC+CGG - chr7.4:74226174-74226193 None:intergenic 55.0%
TTCTCCCCAACCGAAATCGG+TGG - chr7.4:74226136-74226155 None:intergenic 55.0%
! AGAACCGGTTCTGTCTGGAC+CGG + chr7.4:74226152-74226171 MS.gene86677:CDS 55.0%
! ATAGGTGTCGTGTCGGTGTC+CGG + chr7.4:74224662-74224681 MS.gene86677:intron 55.0%
! ACCGGTTTCCTTGCCGGAGA+AGG + chr7.4:74226170-74226189 MS.gene86677:CDS 60.0%
! GTCTGGACCGGTTTCCTTGC+CGG + chr7.4:74226164-74226183 MS.gene86677:CDS 60.0%
! TAGGTGTCGTGTCGGTGTCC+GGG + chr7.4:74224663-74224682 MS.gene86677:intron 60.0%
! TGGGGAGAACCGGTTCTGTC+TGG + chr7.4:74226147-74226166 MS.gene86677:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 74224073 74226299 74224073 ID=MS.gene86677
chr7.4 mRNA 74224073 74226299 74224073 ID=MS.gene86677.t1;Parent=MS.gene86677
chr7.4 exon 74224073 74224522 74224073 ID=MS.gene86677.t1.exon1;Parent=MS.gene86677.t1
chr7.4 CDS 74224073 74224522 74224073 ID=cds.MS.gene86677.t1;Parent=MS.gene86677.t1
chr7.4 exon 74225436 74226299 74225436 ID=MS.gene86677.t1.exon2;Parent=MS.gene86677.t1
chr7.4 CDS 74225436 74226299 74225436 ID=cds.MS.gene86677.t1;Parent=MS.gene86677.t1
Gene Sequence

>MS.gene86677

ATGGAAAATTCTAATTATCAAAATGGCTTCAAACAAATTAATGTAAACCTAACAAGTGAACCAACACTTGTTCCACCATCTGAAGAAACAAAGAAAGGAATGTATTTCCTATCAAACCTTGATCAAAACATTGCTGTAATTGTAAGAACTGTTTATTGCTTCAAAAATGAAGAGAAGGGAAATGAGAATGCTTGTGAAGTGATTAAGGATGCATTGAAGAATGTTCTTGTTCATTATTACCCTCTTGCTGGGAGATTAAGTATAAGCTCAGAGGGTAAACTGATTGTGGATTGTACTGGAGAAGGTGCTCTTTTTGTTGAGGCTGAAGCTAATTGTTCAATGGAAGAAATTGGTGATATTACAAAACCTGATCCAAGGACTCTTGGTATGTTGGTTTATGATATTCCTGATGCTAAACATATTCTTCAGATGCCTCCTTTGGTTGCTCAGGTATGTTTTGTTACTAGTCTATTTTTGCAGTTGGATTTTCTGGATTTGTTAGTTACTTGCTATGAGGCACTGACACAAATATGGACACTGGACACGACATAGATAATAAATTGAGAAAATGAAATGATTCAATGTATTCATAGGTGTCGTGTCGGTGTCCGGGACACCTCAAATCTTAGGAGTGTCTGTACTTCATAGGTTACTTGTAATTTGGGTCCCTAAATTTTTAAATAATTTGGAATTTGGTTTCTAAATTTAGAGACACAAATACAAAGTATTTAAAAGTTTAGAAGAGACCCAATTATAAATGATTTAGAAGTTTAGACACTTGATTAAAAAAATTTAGTTAAGGGATCTAATCAAGTGTATCTTTGATGAGTGGCGAGTTTGACTTTGTTGAAGCTCTAAAGTATAGTTTTTGTCAAAATTATAATGGTTCAGCGTGATTATGACAATTTGACTGTCAATCCAAACATGTGCGAAATATTTTTGACAAATTCAGAAATTCAGTTACAAACTTTTTAAATTCGTAGACCTATTTAAAATTTCTCAAATAGTTTTGCGACATAAATAATTAAACCTTTATTTTTTGTCACTGAAGTTCATGACCAAATGTCTAAGTTCAAAAGTATAGAGACTAGATATATGTAAAGAATAGAAATTGTCACATCTAGCATTTTTCAAGCCCTTCATAGGAATTTATGTATGATGAACATATGGTAGTCATTTTGAATGTATCTCAACTAACATAAAAGGTTCAATCATATAGCAAGTCATCATTTTGTTGAATCATAAAGTTTTATGTCACAGTCGCGTCGCAATATTTTATATCGTGGAGAATTTTTTGAAATATAGTTGATGCGGCCACAACTACAGTTGGAACATGATCACTGAGTAAATTTTTACATTTCAGCTGACCAAGTTCAAATGTGGAGGTTTTTCTCTTGGACTATGCATGAATCACTGTATGTTTGATGGAATTGGAGCTATGGAGTTTGTGAACTCATGGGGAGAACTAGCAAGAGGTTTACCACTTTCTGTGCCTCCTGTTCTCGATCGAAGCATACTAAAGGTCCGCAATCCCCCGAAGATAGAGCATTTGCACCAAGAATTTGCTGACATTGAAGACAAATCCAACACTAGTACCTTATATGAAGATGAAATGGTGTATCGATCATTCTGTTTTGATCCCGAGAAACTAAAAGAATTGAAGAAAAAAGCTTTAGAAGATGATGGCGGTGTTCTTGAATCTTGCACAACATTTGAAGTGCTTTCAGCATTTGTTTGGATAGCTAGAACAAAAGCACTGAAAATGTTACCAGAACAAGAAACAAAGCTTCTTTTTGCTGTTGATGGAAGGGCTAAATTTGAACCAAAACTTCCCAAAGGGTATTTTGGGAATGGAATTATACTAACAAATGCTGTTTGTAAAGCAGGTGAAATTACGAACAAGCCCTTTCCGTATACGGTGAAGGTTATTCAAGATGCAATCAAGATGGTTACTGATAGTTACATGAGATCGGCTATCGATTATTTTGAGGTAACAAGAGCTAGGCCATCTTTGGCTTGTACACTTTTGATCACAACTTGGTCTAGGCTTCCGTTTCATACCACCGATTTCGGTTGGGGAGAACCGGTTCTGTCTGGACCGGTTTCCTTGCCGGAGAAGGAAGTGATTTTGTTCCTTTCACATGGTCAAGAGAGGAGAAGCATTAATGTGCTTCTTGGTTTGCCTGCTCCTGTCATGAAGATCTTCCAAGATTTAATGCAGATATAG
Protein sequence

>MS.gene86677.t1

MENSNYQNGFKQINVNLTSEPTLVPPSEETKKGMYFLSNLDQNIAVIVRTVYCFKNEEKGNENACEVIKDALKNVLVHYYPLAGRLSISSEGKLIVDCTGEGALFVEAEANCSMEEIGDITKPDPRTLGMLVYDIPDAKHILQMPPLVAQLTKFKCGGFSLGLCMNHCMFDGIGAMEFVNSWGELARGLPLSVPPVLDRSILKVRNPPKIEHLHQEFADIEDKSNTSTLYEDEMVYRSFCFDPEKLKELKKKALEDDGGVLESCTTFEVLSAFVWIARTKALKMLPEQETKLLFAVDGRAKFEPKLPKGYFGNGIILTNAVCKAGEITNKPFPYTVKVIQDAIKMVTDSYMRSAIDYFEVTRARPSLACTLLITTWSRLPFHTTDFGWGEPVLSGPVSLPEKEVILFLSHGQERRSINVLLGLPAPVMKIFQDLMQI