AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66891

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66891.t1 MTR_1g053315 98.925 465 5 0 1 465 1 465 0.0 952
MS.gene66891.t1 MTR_3g098980 35.698 451 261 16 18 460 6 435 1.70e-83 264
MS.gene66891.t1 MTR_7g027175 35.101 396 233 15 41 431 36 412 4.31e-71 232
MS.gene66891.t1 MTR_8g096530 34.136 457 261 17 22 463 3 434 3.58e-68 224
MS.gene66891.t1 MTR_3g112180 34.773 440 246 17 25 453 19 428 3.58e-63 212
MS.gene66891.t1 MTR_5g076700 30.394 431 284 11 31 457 20 438 2.49e-59 201
MS.gene66891.t1 MTR_4g007540 31.637 452 272 18 20 457 2 430 2.18e-58 198
MS.gene66891.t1 MTR_2g064495 30.921 456 274 19 20 460 2 431 4.07e-57 195
MS.gene66891.t1 MTR_8g075610 31.306 444 261 19 26 455 2 415 2.54e-56 192
MS.gene66891.t1 MTR_2g105330 30.702 456 275 19 20 460 2 431 8.56e-56 191
MS.gene66891.t1 MTR_1g081660 30.556 432 280 13 31 457 28 444 1.92e-55 191
MS.gene66891.t1 MTR_7g114520 30.385 441 279 15 26 457 19 440 1.24e-54 189
MS.gene66891.t1 MTR_7g114490 31.696 448 281 15 24 460 8 441 1.26e-54 190
MS.gene66891.t1 MTR_3g049150 30.067 449 270 16 18 443 2 429 2.73e-54 188
MS.gene66891.t1 MTR_7g114470 29.358 436 290 11 26 457 19 440 3.04e-54 188
MS.gene66891.t1 MTR_7g114560 29.748 437 285 14 26 457 18 437 9.49e-52 181
MS.gene66891.t1 MTR_1g040175 31.362 389 251 10 26 413 17 390 3.29e-51 180
MS.gene66891.t1 MTR_7g114550 29.977 437 284 14 26 457 18 437 3.47e-51 180
MS.gene66891.t1 MTR_7g084940 28.539 445 293 13 14 453 12 436 5.55e-50 176
MS.gene66891.t1 MTR_4g055170 29.688 448 284 17 26 457 20 452 9.17e-50 176
MS.gene66891.t1 MTR_1g040215 31.378 392 249 11 26 414 17 391 5.10e-49 174
MS.gene66891.t1 MTR_4g127650 29.724 471 282 17 18 462 10 457 3.37e-48 172
MS.gene66891.t1 MTR_3g026950 28.132 455 275 16 18 443 2 433 3.71e-48 172
MS.gene66891.t1 MTR_3g071320 32.104 461 259 23 18 457 5 432 7.29e-48 171
MS.gene66891.t1 MTR_1g040195 29.345 443 264 14 26 463 17 415 1.53e-47 169
MS.gene66891.t1 MTR_4g097090 29.955 444 263 14 47 462 34 457 3.21e-47 169
MS.gene66891.t1 MTR_7g085990 29.032 465 275 15 32 460 11 456 4.61e-46 166
MS.gene66891.t1 MTR_7g085930 28.936 470 269 16 32 460 15 460 6.16e-46 166
MS.gene66891.t1 MTR_1g040320 28.118 441 261 13 26 463 17 404 3.15e-45 163
MS.gene66891.t1 MTR_2g015430 28.929 439 278 18 33 457 16 434 1.31e-44 162
MS.gene66891.t1 MTR_7g084910 29.613 439 262 17 15 443 14 415 2.10e-41 153
MS.gene66891.t1 MTR_7g056087 26.974 456 295 18 18 458 4 436 2.21e-41 153
MS.gene66891.t1 MTR_3g465930 28.884 457 279 16 30 457 19 458 4.00e-39 147
MS.gene66891.t1 MTR_1g040315 27.438 441 256 14 26 463 17 396 1.69e-37 142
MS.gene66891.t1 MTR_4g013050 27.556 450 243 20 19 445 5 394 5.68e-37 140
MS.gene66891.t1 MTR_3g465990 28.416 461 277 20 26 457 18 454 9.78e-37 140
MS.gene66891.t1 MTR_3g465980 28.698 453 286 19 26 457 18 454 4.67e-36 139
MS.gene66891.t1 MTR_3g465910 29.372 446 277 19 34 453 18 451 3.60e-35 137
MS.gene66891.t1 MTR_7g086000 27.651 481 296 18 16 465 2 461 6.22e-35 135
MS.gene66891.t1 MTR_6g053810 28.125 448 276 21 39 457 29 459 5.74e-34 133
MS.gene66891.t1 MTR_7g114510 29.450 309 198 10 158 457 1 298 1.01e-32 127
MS.gene66891.t1 MTR_3g465950 28.540 452 282 20 26 457 18 448 2.98e-31 125
MS.gene66891.t1 MTR_3g465880 27.515 487 279 26 13 457 7 461 1.63e-30 123
MS.gene66891.t1 MTR_7g110610 27.470 415 222 14 23 424 2 350 1.01e-29 120
MS.gene66891.t1 MTR_0502s0010 30.738 244 157 6 117 357 1 235 6.86e-28 111
MS.gene66891.t1 MTR_5g090810 25.871 402 253 15 40 412 29 414 3.11e-25 108
MS.gene66891.t1 MTR_5g090830 25.369 406 250 15 40 412 29 414 4.98e-25 107
MS.gene66891.t1 MTR_5g026320 24.471 425 288 14 35 447 34 437 5.47e-24 104
MS.gene66891.t1 MTR_7g114420 27.536 276 165 9 185 457 3 246 1.28e-23 100
MS.gene66891.t1 MTR_3g465870 31.333 300 179 14 14 301 8 292 1.94e-23 102
MS.gene66891.t1 MTR_3g086020 24.641 418 258 17 14 418 1 374 1.65e-22 99.8
MS.gene66891.t1 MTR_0042s0190 27.273 231 163 4 183 413 1 226 3.09e-22 97.1
MS.gene66891.t1 MTR_5g090880 25.753 365 222 14 78 410 67 414 3.37e-22 99.4
MS.gene66891.t1 MTR_3g450710 25.849 383 241 15 58 412 46 413 2.08e-21 97.1
MS.gene66891.t1 MTR_3g450690 25.762 361 227 14 78 412 72 417 1.96e-20 94.0
MS.gene66891.t1 MTR_8g079980 24.829 439 265 21 20 433 5 403 2.59e-20 93.6
MS.gene66891.t1 MTR_4g094838 24.528 371 229 14 58 410 45 382 2.51e-19 90.5
MS.gene66891.t1 MTR_8g079970 24.009 429 249 14 16 412 1 384 9.05e-19 89.0
MS.gene66891.t1 MTR_0190s0020 24.806 387 248 18 78 446 63 424 1.15e-18 88.6
MS.gene66891.t1 MTR_4g022825 23.571 420 258 18 60 446 33 422 3.16e-18 87.0
MS.gene66891.t1 MTR_8g467580 24.832 447 271 22 14 432 1 410 3.27e-18 87.0
MS.gene66891.t1 MTR_3g049140 28.505 214 128 8 248 443 20 226 1.20e-16 80.1
MS.gene66891.t1 MTR_8g041770 24.331 411 267 19 21 410 4 391 1.88e-16 81.6
MS.gene66891.t1 MTR_4g094842 23.782 349 238 15 78 410 62 398 3.95e-16 80.9
MS.gene66891.t1 MTR_8g467590 24.429 438 273 22 22 432 4 410 4.23e-16 80.9
MS.gene66891.t1 MTR_0042s0190 28.378 148 104 2 266 413 16 161 1.88e-15 75.9
MS.gene66891.t1 MTR_3g088420 25.193 389 245 21 78 447 55 416 1.92e-15 78.6
MS.gene66891.t1 MTR_6g045553 23.371 445 279 20 51 455 44 466 3.24e-15 78.2
MS.gene66891.t1 MTR_4g094832 24.522 314 197 15 115 410 89 380 3.83e-15 77.8
MS.gene66891.t1 MTR_7g114500 32.857 140 83 5 26 164 18 147 1.48e-14 71.2
MS.gene66891.t1 MTR_4g022720 23.944 355 226 15 78 410 55 387 1.96e-14 75.5
MS.gene66891.t1 MTR_3g101960 24.242 462 277 23 21 445 6 431 3.23e-14 75.1
MS.gene66891.t1 MTR_4g022670 25.634 355 226 17 78 410 55 393 4.21e-14 74.3
MS.gene66891.t1 MTR_0042s0230 34.965 143 82 6 26 167 17 149 2.61e-13 67.8
MS.gene66891.t1 MTR_7g114960 22.281 377 251 11 18 381 8 355 4.26e-13 71.6
MS.gene66891.t1 MTR_6g015320 24.138 319 212 13 160 458 143 451 5.20e-13 71.2
MS.gene66891.t1 MTR_8g039950 39.640 111 59 4 26 136 17 119 7.13e-13 65.9
MS.gene66891.t1 MTR_7g014200 22.280 386 255 14 58 409 46 420 1.12e-12 70.1
MS.gene66891.t1 MTR_1g046770 30.000 180 96 8 279 457 36 186 1.23e-12 67.4
MS.gene66891.t1 MTR_1g094790 23.714 447 279 19 16 432 1 415 2.11e-12 69.3
MS.gene66891.t1 MTR_3g026310 24.024 333 219 13 151 465 129 445 2.43e-12 68.9
MS.gene66891.t1 MTR_7g085960 28.079 203 107 8 279 465 15 194 3.06e-12 65.9
MS.gene66891.t1 MTR_4g022800 22.905 358 233 15 78 410 55 394 1.24e-11 67.0
MS.gene66891.t1 MTR_7g014160 22.959 392 250 17 58 409 46 425 5.06e-11 65.1
MS.gene66891.t1 MTR_3g049080 33.333 87 52 2 18 104 2 82 8.94e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66891.t1 AT1G03390 66.087 460 145 6 6 460 5 458 0.0 624
MS.gene66891.t1 AT5G41040 34.607 445 261 15 24 458 15 439 2.90e-74 240
MS.gene66891.t1 AT5G41040 34.607 445 261 15 24 458 31 455 4.11e-74 240
MS.gene66891.t1 AT1G27620 36.156 437 241 18 31 458 19 426 2.87e-73 238
MS.gene66891.t1 AT3G48720 32.639 432 268 14 31 459 19 430 1.48e-67 223
MS.gene66891.t1 AT5G63560 33.333 438 256 18 26 453 9 420 1.21e-65 217
MS.gene66891.t1 AT3G03480 33.333 441 263 16 26 457 27 445 9.39e-58 197
MS.gene66891.t1 AT5G17540 32.960 446 259 17 26 457 17 436 2.43e-56 194
MS.gene66891.t1 AT5G48930 30.512 449 281 15 21 458 3 431 1.05e-54 189
MS.gene66891.t1 AT1G28680 30.464 453 286 19 18 457 4 440 3.74e-52 182
MS.gene66891.t1 AT2G40230 30.599 451 268 18 19 453 3 424 8.02e-52 181
MS.gene66891.t1 AT1G65450 28.390 472 292 18 1 455 1 443 5.23e-45 163
MS.gene66891.t1 AT2G19070 29.684 475 276 20 17 460 3 450 1.14e-44 162
MS.gene66891.t1 AT1G28680 30.831 373 238 15 26 392 12 370 6.49e-44 159
MS.gene66891.t1 AT5G57840 27.609 460 278 14 21 455 3 432 1.18e-39 148
MS.gene66891.t1 AT3G62160 30.198 404 213 17 36 415 20 378 8.31e-39 146
MS.gene66891.t1 AT5G07080 26.866 469 276 15 20 457 13 445 8.66e-39 146
MS.gene66891.t1 AT2G23510 30.044 456 272 21 23 457 18 447 9.74e-36 138
MS.gene66891.t1 AT4G31910 28.281 442 275 19 42 458 32 456 5.24e-35 136
MS.gene66891.t1 AT2G25150 27.778 450 287 19 30 457 16 449 3.65e-32 128
MS.gene66891.t1 AT1G24430 23.624 436 279 18 14 433 1 398 9.09e-26 109
MS.gene66891.t1 AT3G47170 25.567 485 276 22 26 463 20 466 1.60e-25 109
MS.gene66891.t1 AT3G26040 25.854 410 261 15 18 413 3 383 9.24e-25 106
MS.gene66891.t1 AT5G67160 28.895 353 232 13 70 414 48 389 1.16e-22 100
MS.gene66891.t1 AT1G65450 26.621 293 189 11 175 455 1 279 2.10e-22 97.4
MS.gene66891.t1 AT1G31490 23.383 402 274 16 18 409 6 383 2.17e-20 94.0
MS.gene66891.t1 AT1G78990 24.309 362 234 13 70 412 58 398 5.69e-19 89.7
MS.gene66891.t1 AT1G32910 24.533 375 226 15 70 412 52 401 2.25e-18 87.8
MS.gene66891.t1 AT5G23940 23.404 423 272 18 16 409 1 400 3.82e-18 87.4
MS.gene66891.t1 AT3G50280 25.000 356 233 13 78 414 59 399 9.66e-18 85.9
MS.gene66891.t1 AT3G29590 24.691 405 247 15 86 459 69 446 1.41e-17 85.1
MS.gene66891.t1 AT5G16410 24.173 393 249 17 70 438 79 446 1.92e-16 82.0
MS.gene66891.t1 AT5G38130 25.474 369 238 18 70 416 66 419 2.05e-16 81.6
MS.gene66891.t1 AT3G50300 24.866 374 238 15 67 414 43 399 3.50e-16 80.9
MS.gene66891.t1 AT3G30280 24.570 407 248 19 30 411 15 387 1.04e-15 79.7
MS.gene66891.t1 AT5G47980 23.898 431 253 15 14 413 1 387 1.75e-15 79.0
MS.gene66891.t1 AT5G23970 24.396 455 272 20 34 464 11 417 6.50e-15 77.0
MS.gene66891.t1 AT5G07870 24.638 483 297 23 18 465 8 458 2.48e-14 75.5
MS.gene66891.t1 AT3G29670 23.544 412 274 21 58 448 45 436 2.89e-14 75.1
MS.gene66891.t1 AT4G15390 21.957 460 314 17 11 455 1 430 3.19e-13 72.0
MS.gene66891.t1 AT4G15390 21.957 460 314 17 11 455 1 430 3.19e-13 72.0
MS.gene66891.t1 AT3G50280 25.818 275 173 11 156 414 10 269 7.05e-13 69.7
MS.gene66891.t1 AT1G24420 23.114 411 266 20 18 412 5 381 1.24e-12 70.1
MS.gene66891.t1 AT1G24420 23.114 411 266 20 18 412 5 381 1.24e-12 70.1
MS.gene66891.t1 AT5G47950 29.557 203 123 10 30 229 15 200 3.50e-12 68.6
MS.gene66891.t1 AT5G61160 23.947 380 255 17 55 412 38 405 3.11e-11 65.9

Find 95 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTATAATTGTTCTGATTT+TGG 0.098860 2.3:-37619050 MS.gene66891:CDS
AGCCGCAAGATTACGAATTA+AGG 0.212133 2.3:-37619346 MS.gene66891:CDS
CCTTCCTGAGAGAGGGTAAT+AGG 0.223581 2.3:+37619979 None:intergenic
ATGCAATGGCTGCGCATATT+TGG 0.242400 2.3:-37619397 MS.gene66891:CDS
AACTTGTTTGCAGCAGATTC+AGG 0.302184 2.3:+37618906 None:intergenic
CCGCGCTCTAGCTGATGTTT+TGG 0.307482 2.3:-37620005 MS.gene66891:CDS
AGAAGAACAAGGTGCATTAA+TGG 0.315201 2.3:-37619909 MS.gene66891:CDS
ATTACCAGAGACTACTCTTT+TGG 0.318295 2.3:-37619201 MS.gene66891:CDS
TCGATAGGACCAGCATAAAT+TGG 0.322973 2.3:+37619017 None:intergenic
ACTTTGTATTGTTCTTCATT+TGG 0.336455 2.3:+37619800 None:intergenic
TCTCTGTTCCAACAAGGTTC+AGG 0.357662 2.3:+37619617 None:intergenic
CTAGAATCACCTTCCCCTTC+AGG 0.359822 2.3:+37618960 None:intergenic
AATGGTTCCCAAGATGGATT+TGG 0.362060 2.3:+37619836 None:intergenic
ATGGGAGCTATGCAGTTTCT+AGG 0.367373 2.3:-37619683 MS.gene66891:CDS
TGCTGCTCCAAATCCATCTT+GGG 0.372169 2.3:-37619843 MS.gene66891:CDS
CCACCAAACTCAAGTTGCTT+TGG 0.374913 2.3:+37619107 None:intergenic
CAAACTTAACAACGAAACTT+TGG 0.378578 2.3:-37619523 MS.gene66891:CDS
AGGCCAAAGCAACTTGAGTT+TGG 0.383129 2.3:-37619110 MS.gene66891:CDS
TCAAACCTCGATGATGTGAT+TGG 0.383794 2.3:-37620115 MS.gene66891:CDS
TTGCTGCTCCAAATCCATCT+TGG 0.397296 2.3:-37619844 MS.gene66891:CDS
TTTGGAAATTTGAATATCAA+TGG 0.400500 2.3:+37619818 None:intergenic
CTTCCTGAGAGAGGGTAATA+GGG 0.401623 2.3:+37619980 None:intergenic
AATATCAATGGTTCCCAAGA+TGG 0.408999 2.3:+37619830 None:intergenic
ATGTGTGGAAATTTCACTTC+TGG 0.413267 2.3:+37619572 None:intergenic
TCACCTGGTTTGACAGGAAT+AGG 0.422152 2.3:+37620151 None:intergenic
CGGCCAAGTATTTCTGCAAC+CGG 0.424613 2.3:+37620025 None:intergenic
GCTCAACCATTTGATTCTGC+TGG 0.426988 2.3:-37619434 MS.gene66891:CDS
TATTGCTTGCACAACAAGTA+AGG 0.435787 2.3:-37619246 MS.gene66891:CDS
TTACAGAACCTGAACCTTGT+TGG 0.439465 2.3:-37619625 MS.gene66891:CDS
TTACACTTTGTCGAGCTTCA+CGG 0.441582 2.3:+37619177 None:intergenic
CTGCGCATATTTGGAGATCA+TGG 0.441679 2.3:-37619388 MS.gene66891:CDS
AAGGTTTCTGATCTTGTGAA+TGG 0.443284 2.3:-37619227 MS.gene66891:CDS
TTGACAGGAATAGGCTTTGA+GGG 0.448807 2.3:+37620160 None:intergenic
TTGATTATGTTGAAGTTGAT+AGG 0.454882 2.3:-37619130 MS.gene66891:CDS
ATGCGATAACACTTCTGAAT+GGG 0.465756 2.3:+37619491 None:intergenic
AACGGAGAATGTGTTGATAA+TGG 0.468370 2.3:+37620186 None:intergenic
GTTCCCTATTACCCTCTCTC+AGG 0.472374 2.3:-37619983 MS.gene66891:CDS
TTTGACAGGAATAGGCTTTG+AGG 0.477278 2.3:+37620159 None:intergenic
AAACCGGTTGCAGAAATACT+TGG 0.486758 2.3:-37620028 MS.gene66891:CDS
AAAATCCACCATTAAAAGAA+GGG 0.489251 2.3:-37619289 MS.gene66891:CDS
AAACTGTCACCTGGTTTGAC+AGG 0.489983 2.3:+37620145 None:intergenic
AATGCCACTTGTTATAGCTC+AGG 0.506750 2.3:-37619771 MS.gene66891:CDS
AACCTTAATTCGTAATCTTG+CGG 0.509012 2.3:+37619344 None:intergenic
CCAAAGCAACTTGAGTTTGG+TGG 0.511667 2.3:-37619107 MS.gene66891:CDS
GTGCAACCAGCAGAATCAAA+TGG 0.517769 2.3:+37619428 None:intergenic
TTCACTTCTGGCGGATCACG+TGG 0.518723 2.3:+37619584 None:intergenic
TATGCGATAACACTTCTGAA+TGG 0.524313 2.3:+37619490 None:intergenic
TGAAGAACAATACAAAGTGT+TGG 0.527463 2.3:-37619795 MS.gene66891:CDS
TCATTGTATCTGTGATGGAA+TGG 0.531490 2.3:-37619702 MS.gene66891:CDS
TTGCACTACATTTGATGCAA+TGG 0.535105 2.3:-37619411 MS.gene66891:CDS
AGAGGGAGTTCTTGAACCCA+TGG 0.536362 2.3:+37620232 None:intergenic
ACATGTGGTGGATTTAGCCT+CGG 0.541509 2.3:-37619737 MS.gene66891:CDS
CTATGCAGTTTCTAGGAGCA+TGG 0.553170 2.3:-37619676 MS.gene66891:CDS
GTAAACTTACAATTACTCAA+TGG 0.554348 2.3:-37619085 MS.gene66891:CDS
CATTGTATCTGTGATGGAAT+GGG 0.558261 2.3:-37619701 MS.gene66891:CDS
TGCGCATATTTGGAGATCAT+GGG 0.559912 2.3:-37619387 MS.gene66891:CDS
TGGGGTAAGCCAATTTATGC+TGG 0.566093 2.3:-37619026 MS.gene66891:CDS
GGAATATTCAAAGGATTGAG+AGG 0.566846 2.3:+37620214 None:intergenic
AAGCCTATTCCTGTCAAACC+AGG 0.567417 2.3:-37620154 MS.gene66891:CDS
AATACACAAACTTGAGGTGT+AGG 0.567724 2.3:+37618987 None:intergenic
AGTTGATCTCAAATATTCCT+CGG 0.570133 2.3:+37619154 None:intergenic
CGGACCAAAAGAGTAGTCTC+TGG 0.571949 2.3:+37619197 None:intergenic
TATGCAGTTTCTAGGAGCAT+GGG 0.573221 2.3:-37619675 MS.gene66891:CDS
AATGTGTTGATAATGGTGAC+TGG 0.575398 2.3:+37620193 None:intergenic
TGTGGAAATTTCACTTCTGG+CGG 0.576582 2.3:+37619575 None:intergenic
CTCTTAACCTTCCTGAGAGA+GGG 0.576626 2.3:+37619972 None:intergenic
CCTATTACCCTCTCTCAGGA+AGG 0.581255 2.3:-37619979 MS.gene66891:CDS
ATGGTGACTGGAATATTCAA+AGG 0.586441 2.3:+37620205 None:intergenic
GAGTTCATGAGAATTGATGA+AGG 0.588836 2.3:-37619548 MS.gene66891:CDS
CTTTGTCATTGTATCTGTGA+TGG 0.588884 2.3:-37619707 MS.gene66891:CDS
GAAAGATATAAACTGTCACC+TGG 0.589251 2.3:+37620136 None:intergenic
TCTCTTAACCTTCCTGAGAG+AGG 0.592973 2.3:+37619971 None:intergenic
GTTGAGCAAGTGATTTCAAG+TGG 0.594391 2.3:+37619450 None:intergenic
AAAATCTCTCTGTTCCAACA+AGG 0.600364 2.3:+37619611 None:intergenic
AGAAGTGAAATTTCCACACA+TGG 0.607526 2.3:-37619570 MS.gene66891:CDS
CAACACTGAATCAATAAGCA+AGG 0.611495 2.3:+37618875 None:intergenic
GTAACAATCTTCACATGTGG+TGG 0.614672 2.3:-37619749 MS.gene66891:CDS
ATTCTCATGAACTCCATGTG+TGG 0.615728 2.3:+37619557 None:intergenic
GGTGTAGGCGTTAAATCGAT+AGG 0.616810 2.3:+37619002 None:intergenic
AATAGGCTTTGAGGGAGTAA+CGG 0.620871 2.3:+37620168 None:intergenic
AGCACGCGTCTTCACACCGA+CGG 0.621733 2.3:-37620092 MS.gene66891:CDS
GGGGAAGGTGATTCTAGTGG+TGG 0.625246 2.3:-37618954 MS.gene66891:CDS
GTTACCTGAGCTATAACAAG+TGG 0.625798 2.3:+37619767 None:intergenic
AACATAGCTTTATCTGAACT+AGG 0.638913 2.3:-37619872 MS.gene66891:CDS
TTCGGAGAAGAAGAAGAACA+AGG 0.639458 2.3:-37619920 MS.gene66891:CDS
GAATATTCAAAGGATTGAGA+GGG 0.647428 2.3:+37620215 None:intergenic
GAAGGGGAAGGTGATTCTAG+TGG 0.657025 2.3:-37618957 MS.gene66891:CDS
CATCACAGATACAATGACAA+AGG 0.659886 2.3:+37619708 None:intergenic
CAGGTAACAATCTTCACATG+TGG 0.661644 2.3:-37619752 MS.gene66891:CDS
GATTGATAAAAGTAAACCGT+CGG 0.672214 2.3:+37620076 None:intergenic
AGCTCGACAAAGTGTAACCG+AGG 0.683483 2.3:-37619171 MS.gene66891:CDS
CCAAAACATCAGCTAGAGCG+CGG 0.685011 2.3:+37620005 None:intergenic
AATGACAAAGGCGTAAACCG+AGG 0.712450 2.3:+37619720 None:intergenic
GGGAGTTCTTGAACCCATGG+AGG 0.731086 2.3:+37620235 None:intergenic
GTGCTCCAATCACATCATCG+AGG 0.780384 2.3:+37620110 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATAATTGTTCTGATTT+TGG - chr2.3:37620057-37620076 MS.gene66891:CDS 15.0%
!! TTTGGAAATTTGAATATCAA+TGG + chr2.3:37619292-37619311 None:intergenic 20.0%
! AAAAATCCACCATTAAAAGA+AGG - chr2.3:37619817-37619836 MS.gene66891:CDS 25.0%
! AAAATCCACCATTAAAAGAA+GGG - chr2.3:37619818-37619837 MS.gene66891:CDS 25.0%
! ACTTTGTATTGTTCTTCATT+TGG + chr2.3:37619310-37619329 None:intergenic 25.0%
! GTAAACTTACAATTACTCAA+TGG - chr2.3:37620022-37620041 MS.gene66891:CDS 25.0%
!! ATAATTGTTCTGATTTTGGA+TGG - chr2.3:37620061-37620080 MS.gene66891:CDS 25.0%
!! TAATTGTTCTGATTTTGGAT+GGG - chr2.3:37620062-37620081 MS.gene66891:CDS 25.0%
!! TTGATTATGTTGAAGTTGAT+AGG - chr2.3:37619977-37619996 MS.gene66891:CDS 25.0%
!!! GGTAAATTGGAAGTATTTTT+CGG - chr2.3:37619169-37619188 MS.gene66891:CDS 25.0%
!!! TTTGTGTATTTTTACCTGAA+GGG - chr2.3:37620133-37620152 MS.gene66891:CDS 25.0%
AACAGCAAAAACAGGAAAAT+TGG - chr2.3:37619459-37619478 MS.gene66891:CDS 30.0%
AACCTTAATTCGTAATCTTG+CGG + chr2.3:37619766-37619785 None:intergenic 30.0%
AAGTACTTCTTTCCAAAAAC+CGG - chr2.3:37619063-37619082 MS.gene66891:CDS 30.0%
AGAGACGAAAAATGGTAAAT+TGG - chr2.3:37619156-37619175 MS.gene66891:CDS 30.0%
AGTTGATCTCAAATATTCCT+CGG + chr2.3:37619956-37619975 None:intergenic 30.0%
CAAACTTAACAACGAAACTT+TGG - chr2.3:37619584-37619603 MS.gene66891:CDS 30.0%
GAATATTCAAAGGATTGAGA+GGG + chr2.3:37618895-37618914 None:intergenic 30.0%
GATTGATAAAAGTAAACCGT+CGG + chr2.3:37619034-37619053 None:intergenic 30.0%
GGTAAAAATACACAAACTTG+AGG + chr2.3:37620129-37620148 None:intergenic 30.0%
TGAAGAACAATACAAAGTGT+TGG - chr2.3:37619312-37619331 MS.gene66891:CDS 30.0%
! AATTGTTCTGATTTTGGATG+GGG - chr2.3:37620063-37620082 MS.gene66891:CDS 30.0%
! CCATTAAAAGAAGGGTTTTA+CGG - chr2.3:37619826-37619845 MS.gene66891:CDS 30.0%
! TAAAACCCTTCTTTTAATGG+TGG + chr2.3:37619826-37619845 None:intergenic 30.0%
!! AACATAGCTTTATCTGAACT+AGG - chr2.3:37619235-37619254 MS.gene66891:CDS 30.0%
!! GACGGTTTACTTTTATCAAT+CGG - chr2.3:37619033-37619052 MS.gene66891:CDS 30.0%
!! GTTTGTGTATTTTTACCTGA+AGG - chr2.3:37620132-37620151 MS.gene66891:CDS 30.0%
!! TTGTGTATTTTTACCTGAAG+GGG - chr2.3:37620134-37620153 MS.gene66891:CDS 30.0%
AAAATCTCTCTGTTCCAACA+AGG + chr2.3:37619499-37619518 None:intergenic 35.0%
AAGGTTTCTGATCTTGTGAA+TGG - chr2.3:37619880-37619899 MS.gene66891:CDS 35.0%
AATACACAAACTTGAGGTGT+AGG + chr2.3:37620123-37620142 None:intergenic 35.0%
AATATCAATGGTTCCCAAGA+TGG + chr2.3:37619280-37619299 None:intergenic 35.0%
AGAAGAACAAGGTGCATTAA+TGG - chr2.3:37619198-37619217 MS.gene66891:CDS 35.0%
AGAAGTGAAATTTCCACACA+TGG - chr2.3:37619537-37619556 MS.gene66891:CDS 35.0%
AGGTTAAGAGAGACGAAAAA+TGG - chr2.3:37619148-37619167 MS.gene66891:CDS 35.0%
ATGCGATAACACTTCTGAAT+GGG + chr2.3:37619619-37619638 None:intergenic 35.0%
ATGGTGACTGGAATATTCAA+AGG + chr2.3:37618905-37618924 None:intergenic 35.0%
ATGTGTGGAAATTTCACTTC+TGG + chr2.3:37619538-37619557 None:intergenic 35.0%
CAACACTGAATCAATAAGCA+AGG + chr2.3:37620235-37620254 None:intergenic 35.0%
CATCACAGATACAATGACAA+AGG + chr2.3:37619402-37619421 None:intergenic 35.0%
CTTTGTCATTGTATCTGTGA+TGG - chr2.3:37619400-37619419 MS.gene66891:CDS 35.0%
GAAAGATATAAACTGTCACC+TGG + chr2.3:37618974-37618993 None:intergenic 35.0%
GAGTTCATGAGAATTGATGA+AGG - chr2.3:37619559-37619578 MS.gene66891:CDS 35.0%
GGAATATTCAAAGGATTGAG+AGG + chr2.3:37618896-37618915 None:intergenic 35.0%
TATGCGATAACACTTCTGAA+TGG + chr2.3:37619620-37619639 None:intergenic 35.0%
TATTGCTTGCACAACAAGTA+AGG - chr2.3:37619861-37619880 MS.gene66891:CDS 35.0%
TTGCACTACATTTGATGCAA+TGG - chr2.3:37619696-37619715 MS.gene66891:CDS 35.0%
! AACGGAGAATGTGTTGATAA+TGG + chr2.3:37618924-37618943 None:intergenic 35.0%
! ATTACCAGAGACTACTCTTT+TGG - chr2.3:37619906-37619925 MS.gene66891:CDS 35.0%
! CATTGTATCTGTGATGGAAT+GGG - chr2.3:37619406-37619425 MS.gene66891:CDS 35.0%
! CCGTAAAACCCTTCTTTTAA+TGG + chr2.3:37619829-37619848 None:intergenic 35.0%
! TCATTGTATCTGTGATGGAA+TGG - chr2.3:37619405-37619424 MS.gene66891:CDS 35.0%
!! AATGTGTTGATAATGGTGAC+TGG + chr2.3:37618917-37618936 None:intergenic 35.0%
AAACCGGTTGCAGAAATACT+TGG - chr2.3:37619079-37619098 MS.gene66891:CDS 40.0%
AGCCGCAAGATTACGAATTA+AGG - chr2.3:37619761-37619780 MS.gene66891:CDS 40.0%
ATTCTCATGAACTCCATGTG+TGG + chr2.3:37619553-37619572 None:intergenic 40.0%
CAGGTAACAATCTTCACATG+TGG - chr2.3:37619355-37619374 MS.gene66891:CDS 40.0%
GTAACAATCTTCACATGTGG+TGG - chr2.3:37619358-37619377 MS.gene66891:CDS 40.0%
GTTACCTGAGCTATAACAAG+TGG + chr2.3:37619343-37619362 None:intergenic 40.0%
GTTGAGCAAGTGATTTCAAG+TGG + chr2.3:37619660-37619679 None:intergenic 40.0%
TCAAACCTCGATGATGTGAT+TGG - chr2.3:37618992-37619011 MS.gene66891:CDS 40.0%
TCGATAGGACCAGCATAAAT+TGG + chr2.3:37620093-37620112 None:intergenic 40.0%
TGCGCATATTTGGAGATCAT+GGG - chr2.3:37619720-37619739 MS.gene66891:CDS 40.0%
TGTGGAAATTTCACTTCTGG+CGG + chr2.3:37619535-37619554 None:intergenic 40.0%
TTACACTTTGTCGAGCTTCA+CGG + chr2.3:37619933-37619952 None:intergenic 40.0%
TTACAGAACCTGAACCTTGT+TGG - chr2.3:37619482-37619501 MS.gene66891:CDS 40.0%
TTCGGAGAAGAAGAAGAACA+AGG - chr2.3:37619187-37619206 MS.gene66891:CDS 40.0%
TTTGACAGGAATAGGCTTTG+AGG + chr2.3:37618951-37618970 None:intergenic 40.0%
! AACTTGTTTGCAGCAGATTC+AGG + chr2.3:37620204-37620223 None:intergenic 40.0%
! AATGGTTCCCAAGATGGATT+TGG + chr2.3:37619274-37619293 None:intergenic 40.0%
! AGAAGGGTTTTACGGTAATG+TGG - chr2.3:37619834-37619853 MS.gene66891:CDS 40.0%
! TATGCAGTTTCTAGGAGCAT+GGG - chr2.3:37619432-37619451 MS.gene66891:CDS 40.0%
! TTGACAGGAATAGGCTTTGA+GGG + chr2.3:37618950-37618969 None:intergenic 40.0%
!! AATAGGCTTTGAGGGAGTAA+CGG + chr2.3:37618942-37618961 None:intergenic 40.0%
!! AATGCCACTTGTTATAGCTC+AGG - chr2.3:37619336-37619355 MS.gene66891:CDS 40.0%
!! GTATTTCTGCAACCGGTTTT+TGG + chr2.3:37619078-37619097 None:intergenic 40.0%
!! GTATTTTTACCTGAAGGGGA+AGG - chr2.3:37620138-37620157 MS.gene66891:CDS 40.0%
AAGCCTATTCCTGTCAAACC+AGG - chr2.3:37618953-37618972 MS.gene66891:CDS 45.0%
AGGCCAAAGCAACTTGAGTT+TGG - chr2.3:37619997-37620016 MS.gene66891:CDS 45.0%
ATGCAATGGCTGCGCATATT+TGG - chr2.3:37619710-37619729 MS.gene66891:CDS 45.0%
ATGGGAGCTATGCAGTTTCT+AGG - chr2.3:37619424-37619443 MS.gene66891:CDS 45.0%
CCAAAGCAACTTGAGTTTGG+TGG - chr2.3:37620000-37620019 MS.gene66891:CDS 45.0%
CTCTTAACCTTCCTGAGAGA+GGG + chr2.3:37619138-37619157 None:intergenic 45.0%
CTGCGCATATTTGGAGATCA+TGG - chr2.3:37619719-37619738 MS.gene66891:CDS 45.0%
CTTCCTGAGAGAGGGTAATA+GGG + chr2.3:37619130-37619149 None:intergenic 45.0%
GCTCAACCATTTGATTCTGC+TGG - chr2.3:37619673-37619692 MS.gene66891:CDS 45.0%
GGTGTAGGCGTTAAATCGAT+AGG + chr2.3:37620108-37620127 None:intergenic 45.0%
GTGCAACCAGCAGAATCAAA+TGG + chr2.3:37619682-37619701 None:intergenic 45.0%
TCTCTGTTCCAACAAGGTTC+AGG + chr2.3:37619493-37619512 None:intergenic 45.0%
TCTCTTAACCTTCCTGAGAG+AGG + chr2.3:37619139-37619158 None:intergenic 45.0%
TGCTGCTCCAAATCCATCTT+GGG - chr2.3:37619264-37619283 MS.gene66891:CDS 45.0%
TGGGGTAAGCCAATTTATGC+TGG - chr2.3:37620081-37620100 MS.gene66891:CDS 45.0%
TTGCTGCTCCAAATCCATCT+TGG - chr2.3:37619263-37619282 MS.gene66891:CDS 45.0%
! AAACTGTCACCTGGTTTGAC+AGG + chr2.3:37618965-37618984 None:intergenic 45.0%
! AATGACAAAGGCGTAAACCG+AGG + chr2.3:37619390-37619409 None:intergenic 45.0%
! CCACCAAACTCAAGTTGCTT+TGG + chr2.3:37620003-37620022 None:intergenic 45.0%
! CTATGCAGTTTCTAGGAGCA+TGG - chr2.3:37619431-37619450 MS.gene66891:CDS 45.0%
! TCACCTGGTTTGACAGGAAT+AGG + chr2.3:37618959-37618978 None:intergenic 45.0%
!! ACATGTGGTGGATTTAGCCT+CGG - chr2.3:37619370-37619389 MS.gene66891:CDS 45.0%
AGAGGGAGTTCTTGAACCCA+TGG + chr2.3:37618878-37618897 None:intergenic 50.0%
AGCTCGACAAAGTGTAACCG+AGG - chr2.3:37619936-37619955 MS.gene66891:CDS 50.0%
CCAAAACATCAGCTAGAGCG+CGG + chr2.3:37619105-37619124 None:intergenic 50.0%
CCTATTACCCTCTCTCAGGA+AGG - chr2.3:37619128-37619147 MS.gene66891:CDS 50.0%
CCTTCCTGAGAGAGGGTAAT+AGG + chr2.3:37619131-37619150 None:intergenic 50.0%
CGGACCAAAAGAGTAGTCTC+TGG + chr2.3:37619913-37619932 None:intergenic 50.0%
CTAGAATCACCTTCCCCTTC+AGG + chr2.3:37620150-37620169 None:intergenic 50.0%
GAAGGGGAAGGTGATTCTAG+TGG - chr2.3:37620150-37620169 MS.gene66891:CDS 50.0%
GTGCTCCAATCACATCATCG+AGG + chr2.3:37619000-37619019 None:intergenic 50.0%
GTTCCCTATTACCCTCTCTC+AGG - chr2.3:37619124-37619143 MS.gene66891:CDS 50.0%
TGGGCTGCAACAGCAAAAAC+AGG - chr2.3:37619451-37619470 MS.gene66891:CDS 50.0%
! CGGCCAAGTATTTCTGCAAC+CGG + chr2.3:37619085-37619104 None:intergenic 50.0%
GGGAGTTCTTGAACCCATGG+AGG + chr2.3:37618875-37618894 None:intergenic 55.0%
GGGGAAGGTGATTCTAGTGG+TGG - chr2.3:37620153-37620172 MS.gene66891:CDS 55.0%
! CCGCGCTCTAGCTGATGTTT+TGG - chr2.3:37619102-37619121 MS.gene66891:CDS 55.0%
! TTCACTTCTGGCGGATCACG+TGG + chr2.3:37619526-37619545 None:intergenic 55.0%
AGCACGCGTCTTCACACCGA+CGG - chr2.3:37619015-37619034 MS.gene66891:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 37618866 37620263 37618866 ID=MS.gene66891
chr2.3 mRNA 37618866 37620263 37618866 ID=MS.gene66891.t1;Parent=MS.gene66891
chr2.3 exon 37618866 37620263 37618866 ID=MS.gene66891.t1.exon1;Parent=MS.gene66891.t1
chr2.3 CDS 37618866 37620263 37618866 ID=cds.MS.gene66891.t1;Parent=MS.gene66891.t1
Gene Sequence

>MS.gene66891

ATGGCTCCTCCATGGGTTCAAGAACTCCCTCTCAATCCTTTGAATATTCCAGTCACCATTATCAACACATTCTCCGTTACTCCCTCAAAGCCTATTCCTGTCAAACCAGGTGACAGTTTATATCTTTCAAACCTCGATGATGTGATTGGAGCACGCGTCTTCACACCGACGGTTTACTTTTATCAATCGGACAATTCAAGTACTTCTTTCCAAAAACCGGTTGCAGAAATACTTGGCCGCGCTCTAGCTGATGTTTTGGTTCCCTATTACCCTCTCTCAGGAAGGTTAAGAGAGACGAAAAATGGTAAATTGGAAGTATTTTTCGGAGAAGAAGAAGAACAAGGTGCATTAATGGTTGAAGCAAGATCAAACATAGCTTTATCTGAACTAGGTGATTTTGCTGCTCCAAATCCATCTTGGGAACCATTGATATTCAAATTTCCAAATGAAGAACAATACAAAGTGTTGGAAATGCCACTTGTTATAGCTCAGGTAACAATCTTCACATGTGGTGGATTTAGCCTCGGTTTACGCCTTTGTCATTGTATCTGTGATGGAATGGGAGCTATGCAGTTTCTAGGAGCATGGGCTGCAACAGCAAAAACAGGAAAATTGGTTACAGAACCTGAACCTTGTTGGAACAGAGAGATTTTCAAGCCACGTGATCCGCCAGAAGTGAAATTTCCACACATGGAGTTCATGAGAATTGATGAAGGATCAAACTTAACAACGAAACTTTGGAAAACAAAGCCCATTCAGAAGTGTTATCGCATAAGACGCGAGTTTCAAAACCACTTGAAATCACTTGCTCAACCATTTGATTCTGCTGGTTGCACTACATTTGATGCAATGGCTGCGCATATTTGGAGATCATGGGTTAAAGCTCTTGATGTTAAGCCGCAAGATTACGAATTAAGGTTAACATTTTCAGTTAATGCTAGACAGAAGCTTAAAAATCCACCATTAAAAGAAGGGTTTTACGGTAATGTGGTTTGTATTGCTTGCACAACAAGTAAGGTTTCTGATCTTGTGAATGGAAAATTACCAGAGACTACTCTTTTGGTCCGTGAAGCTCGACAAAGTGTAACCGAGGAATATTTGAGATCAACTGTTGATTATGTTGAAGTTGATAGGCCAAAGCAACTTGAGTTTGGTGGTAAACTTACAATTACTCAATGGACAAGATTTTCTATTTATAATTGTTCTGATTTTGGATGGGGTAAGCCAATTTATGCTGGTCCTATCGATTTAACGCCTACACCTCAAGTTTGTGTATTTTTACCTGAAGGGGAAGGTGATTCTAGTGGTGGTTCTATGATTGTTTGCATTTGCTTGCCTGAATCTGCTGCAAACAAGTTTGCACAAGCCTTGCTTATTGATTCAGTGTTGTTGACATAG
Protein sequence

>MS.gene66891.t1

MAPPWVQELPLNPLNIPVTIINTFSVTPSKPIPVKPGDSLYLSNLDDVIGARVFTPTVYFYQSDNSSTSFQKPVAEILGRALADVLVPYYPLSGRLRETKNGKLEVFFGEEEEQGALMVEARSNIALSELGDFAAPNPSWEPLIFKFPNEEQYKVLEMPLVIAQVTIFTCGGFSLGLRLCHCICDGMGAMQFLGAWAATAKTGKLVTEPEPCWNREIFKPRDPPEVKFPHMEFMRIDEGSNLTTKLWKTKPIQKCYRIRREFQNHLKSLAQPFDSAGCTTFDAMAAHIWRSWVKALDVKPQDYELRLTFSVNARQKLKNPPLKEGFYGNVVCIACTTSKVSDLVNGKLPETTLLVREARQSVTEEYLRSTVDYVEVDRPKQLEFGGKLTITQWTRFSIYNCSDFGWGKPIYAGPIDLTPTPQVCVFLPEGEGDSSGGSMIVCICLPESAANKFAQALLIDSVLLT