AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069436

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069436.t1 MTR_1g053315 99.355 465 3 0 1 465 1 465 0.0 956
MS.gene069436.t1 MTR_3g098980 35.033 451 264 16 18 460 6 435 1.12e-81 259
MS.gene069436.t1 MTR_7g027175 35.354 396 232 15 41 431 36 412 1.09e-70 231
MS.gene069436.t1 MTR_8g096530 34.660 427 244 15 22 435 3 407 7.16e-67 221
MS.gene069436.t1 MTR_3g112180 34.773 440 246 17 25 453 19 428 2.97e-62 209
MS.gene069436.t1 MTR_5g076700 30.394 431 284 11 31 457 20 438 3.08e-58 199
MS.gene069436.t1 MTR_4g007540 31.818 440 263 18 20 445 2 418 3.14e-57 195
MS.gene069436.t1 MTR_8g075610 32.203 413 240 17 47 445 19 405 8.64e-57 194
MS.gene069436.t1 MTR_2g064495 30.995 442 265 18 20 446 2 418 9.80e-57 194
MS.gene069436.t1 MTR_2g105330 30.769 442 266 18 20 446 2 418 2.54e-55 190
MS.gene069436.t1 MTR_1g081660 30.324 432 281 13 31 457 28 444 4.08e-54 187
MS.gene069436.t1 MTR_7g114490 31.364 440 278 14 24 453 8 433 7.49e-54 188
MS.gene069436.t1 MTR_3g049150 30.067 449 270 16 18 443 2 429 1.02e-53 187
MS.gene069436.t1 MTR_7g114470 29.358 436 290 11 26 457 19 440 1.51e-53 186
MS.gene069436.t1 MTR_7g114520 29.861 432 286 12 31 457 21 440 2.91e-53 186
MS.gene069436.t1 MTR_7g114550 32.041 387 245 12 31 413 20 392 1.06e-50 179
MS.gene069436.t1 MTR_7g114560 29.519 437 286 14 26 457 18 437 1.29e-50 178
MS.gene069436.t1 MTR_1g040175 31.620 389 250 10 26 413 17 390 1.51e-50 178
MS.gene069436.t1 MTR_3g026950 28.132 455 275 16 18 443 2 433 2.06e-49 175
MS.gene069436.t1 MTR_1g040215 31.122 392 250 11 26 414 17 391 2.14e-49 175
MS.gene069436.t1 MTR_4g055170 29.103 457 296 16 17 457 8 452 5.14e-49 174
MS.gene069436.t1 MTR_7g084940 28.953 449 286 14 14 453 12 436 5.81e-49 174
MS.gene069436.t1 MTR_3g071320 32.251 462 257 24 18 457 5 432 9.13e-49 173
MS.gene069436.t1 MTR_4g127650 29.787 470 283 17 18 462 10 457 6.65e-48 171
MS.gene069436.t1 MTR_4g097090 30.435 460 269 16 33 462 19 457 1.77e-47 170
MS.gene069436.t1 MTR_1g040195 29.120 443 265 14 26 463 17 415 2.27e-47 169
MS.gene069436.t1 MTR_2g015430 29.596 446 279 19 26 457 10 434 1.64e-45 164
MS.gene069436.t1 MTR_7g085990 28.601 479 282 17 18 460 2 456 4.85e-45 164
MS.gene069436.t1 MTR_1g040320 28.345 441 260 13 26 463 17 404 8.98e-45 162
MS.gene069436.t1 MTR_7g085930 28.512 484 276 18 18 460 6 460 1.02e-44 163
MS.gene069436.t1 MTR_7g084910 29.613 439 262 17 15 443 14 415 5.56e-41 152
MS.gene069436.t1 MTR_7g056087 26.974 456 295 18 18 458 4 436 7.04e-41 152
MS.gene069436.t1 MTR_3g465930 28.665 457 280 17 30 457 19 458 5.30e-39 147
MS.gene069436.t1 MTR_1g040315 27.438 441 256 15 26 463 17 396 2.35e-37 141
MS.gene069436.t1 MTR_4g013050 28.326 466 247 22 19 457 5 410 2.69e-37 141
MS.gene069436.t1 MTR_3g465990 28.139 462 277 20 26 457 18 454 2.95e-37 142
MS.gene069436.t1 MTR_3g465980 28.355 462 276 20 26 457 18 454 7.40e-37 141
MS.gene069436.t1 MTR_7g086000 27.443 481 297 18 16 465 2 461 6.73e-35 135
MS.gene069436.t1 MTR_3g465910 28.942 463 289 21 13 453 7 451 2.25e-34 135
MS.gene069436.t1 MTR_6g053810 28.856 402 253 19 77 457 70 459 5.21e-33 130
MS.gene069436.t1 MTR_7g114510 29.450 309 198 10 158 457 1 298 1.86e-31 123
MS.gene069436.t1 MTR_3g465950 28.670 436 262 20 26 439 18 426 4.80e-31 125
MS.gene069436.t1 MTR_3g465880 28.541 473 292 26 13 457 7 461 2.18e-30 123
MS.gene069436.t1 MTR_7g110610 28.771 358 188 12 75 424 52 350 8.56e-30 120
MS.gene069436.t1 MTR_0502s0010 30.738 244 157 6 117 357 1 235 2.70e-28 112
MS.gene069436.t1 MTR_5g026320 24.590 427 289 14 35 449 34 439 4.60e-25 107
MS.gene069436.t1 MTR_5g090830 25.123 406 251 15 40 412 29 414 8.59e-25 107
MS.gene069436.t1 MTR_5g090810 25.436 401 256 14 40 412 29 414 1.10e-24 106
MS.gene069436.t1 MTR_3g465870 30.719 306 173 15 14 301 8 292 1.58e-23 102
MS.gene069436.t1 MTR_3g086020 25.120 418 256 17 14 418 1 374 1.25e-22 100
MS.gene069436.t1 MTR_7g114420 27.536 276 165 9 185 457 3 246 1.46e-22 97.4
MS.gene069436.t1 MTR_5g090880 25.479 365 223 14 78 410 67 414 2.85e-22 99.8
MS.gene069436.t1 MTR_0042s0190 27.706 231 162 4 183 413 1 226 3.89e-22 96.7
MS.gene069436.t1 MTR_3g450710 25.849 383 241 15 58 412 46 413 5.37e-21 95.9
MS.gene069436.t1 MTR_8g079980 25.285 439 263 21 20 433 5 403 1.05e-20 94.7
MS.gene069436.t1 MTR_3g450690 25.762 361 227 14 78 412 72 417 2.83e-20 93.6
MS.gene069436.t1 MTR_8g079970 25.000 424 251 14 16 412 1 384 2.05e-19 90.9
MS.gene069436.t1 MTR_4g094838 24.528 371 229 14 58 410 45 382 6.96e-19 89.0
MS.gene069436.t1 MTR_8g467580 24.330 448 270 22 16 432 1 410 1.49e-18 88.2
MS.gene069436.t1 MTR_0190s0020 24.806 387 248 18 78 446 63 424 1.91e-18 87.8
MS.gene069436.t1 MTR_4g022825 23.571 420 258 18 60 446 33 422 6.48e-18 86.3
MS.gene069436.t1 MTR_4g094835 24.581 358 220 17 78 410 64 396 6.79e-17 83.2
MS.gene069436.t1 MTR_3g049140 28.505 214 128 8 248 443 20 226 7.07e-17 80.5
MS.gene069436.t1 MTR_8g041770 24.331 411 267 19 21 410 4 391 3.32e-16 80.9
MS.gene069436.t1 MTR_4g094842 23.563 348 240 14 78 410 62 398 4.40e-16 80.5
MS.gene069436.t1 MTR_8g467590 24.429 438 273 22 22 432 4 410 7.45e-16 80.1
MS.gene069436.t1 MTR_0042s0190 28.378 148 104 2 266 413 16 161 2.09e-15 75.9
MS.gene069436.t1 MTR_3g088420 25.193 389 245 21 78 447 55 416 2.45e-15 78.2
MS.gene069436.t1 MTR_6g045553 23.559 399 246 19 51 409 44 423 4.09e-15 77.8
MS.gene069436.t1 MTR_4g094832 24.522 314 197 16 115 410 89 380 4.84e-15 77.4
MS.gene069436.t1 MTR_3g101960 24.359 468 281 23 14 445 1 431 1.62e-14 75.9
MS.gene069436.t1 MTR_7g114500 33.077 130 79 4 36 164 25 147 1.85e-14 71.2
MS.gene069436.t1 MTR_4g022720 23.944 355 226 15 78 410 55 387 3.35e-14 74.7
MS.gene069436.t1 MTR_2g007880 22.482 427 271 17 16 416 15 407 3.99e-14 74.7
MS.gene069436.t1 MTR_4g022670 25.634 355 226 17 78 410 55 393 7.20e-14 73.9
MS.gene069436.t1 MTR_0042s0230 35.664 143 81 6 26 167 17 149 1.08e-13 68.9
MS.gene069436.t1 MTR_6g015320 25.168 298 194 13 160 437 143 431 1.28e-13 73.2
MS.gene069436.t1 MTR_7g014200 22.539 386 254 14 58 409 46 420 2.86e-13 72.0
MS.gene069436.t1 MTR_7g085960 28.571 203 106 8 279 465 15 194 3.23e-13 68.9
MS.gene069436.t1 MTR_8g039950 38.739 111 60 4 26 136 17 119 7.93e-13 65.9
MS.gene069436.t1 MTR_3g026310 23.423 333 221 12 151 465 129 445 7.99e-13 70.5
MS.gene069436.t1 MTR_1g046770 30.000 180 96 8 279 457 36 186 4.96e-12 65.5
MS.gene069436.t1 MTR_4g022800 22.905 358 233 15 78 410 55 394 5.46e-12 67.8
MS.gene069436.t1 MTR_7g114960 21.485 377 254 11 18 381 8 355 6.07e-12 67.8
MS.gene069436.t1 MTR_1g094790 23.348 454 268 20 18 432 3 415 6.63e-12 67.8
MS.gene069436.t1 MTR_7g014160 23.214 392 249 17 58 409 46 425 1.22e-11 67.0
MS.gene069436.t1 MTR_2g045550 28.049 164 96 7 18 180 4 146 1.30e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069436.t1 AT1G03390 65.870 460 146 6 6 460 5 458 0.0 620
MS.gene069436.t1 AT5G41040 35.225 423 249 14 41 458 37 439 4.86e-73 237
MS.gene069436.t1 AT5G41040 34.813 428 244 16 41 458 53 455 8.59e-73 237
MS.gene069436.t1 AT1G27620 37.123 431 230 19 26 447 17 415 3.53e-72 235
MS.gene069436.t1 AT3G48720 32.176 432 270 14 31 459 19 430 3.67e-66 219
MS.gene069436.t1 AT5G63560 33.105 438 257 18 26 453 9 420 3.31e-64 214
MS.gene069436.t1 AT3G03480 33.333 441 263 16 26 457 27 445 1.41e-57 197
MS.gene069436.t1 AT5G17540 32.735 446 260 17 26 457 17 436 5.26e-55 190
MS.gene069436.t1 AT5G48930 30.963 436 270 15 21 445 3 418 1.59e-54 188
MS.gene069436.t1 AT2G40230 32.251 431 247 18 26 440 13 414 4.50e-51 179
MS.gene069436.t1 AT1G28680 30.365 438 277 18 33 457 18 440 4.85e-51 179
MS.gene069436.t1 AT1G65450 28.390 472 292 18 1 455 1 443 2.94e-44 161
MS.gene069436.t1 AT2G19070 29.913 458 267 19 17 445 3 435 7.68e-44 160
MS.gene069436.t1 AT1G28680 30.601 366 235 14 33 392 18 370 1.32e-43 158
MS.gene069436.t1 AT3G62160 30.077 389 233 15 36 415 20 378 5.83e-39 146
MS.gene069436.t1 AT5G57840 28.319 452 267 15 30 455 12 432 7.48e-39 146
MS.gene069436.t1 AT5G07080 25.966 466 284 13 20 457 13 445 4.75e-37 141
MS.gene069436.t1 AT4G31910 28.507 442 274 19 42 458 32 456 2.37e-35 137
MS.gene069436.t1 AT2G23510 29.386 456 275 21 23 457 18 447 1.06e-34 135
MS.gene069436.t1 AT2G25150 27.540 443 287 18 33 457 23 449 2.82e-31 125
MS.gene069436.t1 AT1G24430 22.936 436 282 18 14 433 1 398 5.81e-25 107
MS.gene069436.t1 AT3G26040 25.610 410 262 14 18 413 3 383 1.12e-24 106
MS.gene069436.t1 AT3G47170 25.155 485 278 22 26 463 20 466 1.24e-24 106
MS.gene069436.t1 AT1G65450 26.621 293 189 11 175 455 1 279 3.07e-22 97.1
MS.gene069436.t1 AT5G67160 28.612 353 233 13 70 414 48 389 5.38e-22 98.6
MS.gene069436.t1 AT1G31490 22.637 402 277 16 18 409 6 383 9.12e-20 92.0
MS.gene069436.t1 AT1G78990 24.242 363 235 13 70 413 58 399 7.14e-19 89.4
MS.gene069436.t1 AT5G23940 23.404 423 272 18 16 409 1 400 4.74e-18 87.0
MS.gene069436.t1 AT1G32910 24.533 375 226 15 70 412 52 401 4.77e-18 86.7
MS.gene069436.t1 AT3G50280 25.281 356 232 13 78 414 59 399 7.49e-18 86.3
MS.gene069436.t1 AT3G29590 24.938 401 239 16 86 453 69 440 4.74e-17 83.6
MS.gene069436.t1 AT5G38130 25.474 369 238 18 70 416 66 419 7.20e-17 83.2
MS.gene069436.t1 AT5G16410 24.173 393 249 17 70 438 79 446 3.26e-16 81.3
MS.gene069436.t1 AT3G50300 24.798 371 236 15 70 414 46 399 1.22e-15 79.3
MS.gene069436.t1 AT5G47980 24.130 431 252 15 14 413 1 387 1.70e-15 79.0
MS.gene069436.t1 AT3G30280 24.324 407 249 19 30 411 15 387 2.40e-15 78.6
MS.gene069436.t1 AT5G23970 24.703 421 258 18 33 432 13 395 4.26e-15 77.4
MS.gene069436.t1 AT5G07870 25.183 409 250 20 70 448 64 446 7.28e-15 77.0
MS.gene069436.t1 AT3G29670 23.798 416 262 21 58 445 45 433 3.39e-14 74.7
MS.gene069436.t1 AT4G15390 22.596 416 281 15 11 411 1 390 3.43e-13 71.6
MS.gene069436.t1 AT4G15390 22.596 416 281 15 11 411 1 390 3.43e-13 71.6
MS.gene069436.t1 AT3G50280 26.182 275 172 11 156 414 10 269 6.03e-13 70.1
MS.gene069436.t1 AT1G24420 23.227 409 268 20 18 412 5 381 2.36e-12 68.9
MS.gene069436.t1 AT1G24420 23.227 409 268 20 18 412 5 381 2.36e-12 68.9
MS.gene069436.t1 AT5G47950 28.713 202 126 9 30 229 15 200 2.95e-12 68.9
MS.gene069436.t1 AT5G61160 22.812 377 263 15 55 412 38 405 1.76e-11 66.6
MS.gene069436.t1 AT5G39090 22.199 473 310 17 14 460 1 441 2.62e-11 65.9

Find 92 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTATAATTGTTCTGATTT+TGG 0.098860 2.2:-30642524 MS.gene069436:CDS
AGCCGCAAGATTACGAATTA+AGG 0.212133 2.2:-30642820 MS.gene069436:CDS
ATGCAATGGCTGCACATATT+TGG 0.230199 2.2:-30642871 MS.gene069436:CDS
CCTTCCAGAGAGAGGGTAAT+AGG 0.259302 2.2:+30643453 None:intergenic
CCGCGCTCTAGCTGATGTTT+TGG 0.307482 2.2:-30643479 MS.gene069436:CDS
AGAAGAACAAGGTGCATTAA+TGG 0.315201 2.2:-30643383 MS.gene069436:CDS
TCAATAGGACCAGCATAAAT+TGG 0.316153 2.2:+30642491 None:intergenic
ATTACCAGAGACTACTCTTT+TGG 0.326454 2.2:-30642675 MS.gene069436:CDS
AACTTGTTCGCAGCAAATTC+AGG 0.331842 2.2:+30642380 None:intergenic
ACTTTGTATTGTTCTTCATT+TGG 0.336455 2.2:+30643274 None:intergenic
TTGACAGGAATAGGCTTTGA+TGG 0.354952 2.2:+30643634 None:intergenic
TCTCTGTTCCAACAAGGTTC+AGG 0.357662 2.2:+30643091 None:intergenic
AATGGTTCCCAAGATGGATT+TGG 0.362060 2.2:+30643310 None:intergenic
CCATAAAACCCTTCTTTCAA+TGG 0.366018 2.2:+30642755 None:intergenic
ATGGGAGCTATGCAGTTTCT+AGG 0.367373 2.2:-30643157 MS.gene069436:CDS
TGCTGCTCCAAATCCATCTT+GGG 0.372169 2.2:-30643317 MS.gene069436:CDS
CCACCAAACTCAAGTTGCTT+TGG 0.374913 2.2:+30642581 None:intergenic
CTAGAATCACCTTCTCCTTC+AGG 0.380164 2.2:+30642434 None:intergenic
AGGCCAAAGCAACTTGAGTT+TGG 0.383129 2.2:-30642584 MS.gene069436:CDS
TTGCTGCTCCAAATCCATCT+TGG 0.397296 2.2:-30643318 MS.gene069436:CDS
TCAAACCTCGATGACGTGAT+TGG 0.399128 2.2:-30643589 MS.gene069436:CDS
CAAACTTAACAATGAAACTT+TGG 0.400300 2.2:-30642997 MS.gene069436:CDS
TTTGGAAATTTGAATATCAA+TGG 0.400500 2.2:+30643292 None:intergenic
ATGTGTGGAAATTTCACTTC+TGG 0.401320 2.2:+30643046 None:intergenic
CTTCCAGAGAGAGGGTAATA+GGG 0.404106 2.2:+30643454 None:intergenic
ATGCGATAACACTTCTGAAC+AGG 0.407661 2.2:+30642965 None:intergenic
AATATCAATGGTTCCCAAGA+TGG 0.408999 2.2:+30643304 None:intergenic
GGTGTAGGTGTTAAATCAAT+AGG 0.417668 2.2:+30642476 None:intergenic
CGGCCAAGTATTTCTGCAAC+CGG 0.424613 2.2:+30643499 None:intergenic
CTGCACATATTTGGAGATCA+TGG 0.426583 2.2:-30642862 MS.gene069436:CDS
TCACCTGGTTTGACAGGAAT+AGG 0.426764 2.2:+30643625 None:intergenic
GTTCCCTATTACCCTCTCTC+TGG 0.430323 2.2:-30643457 MS.gene069436:CDS
GCTCAACCATTTGACTCTGC+TGG 0.430629 2.2:-30642908 MS.gene069436:CDS
TATTGCTTGCACAACAAGTA+AGG 0.435787 2.2:-30642720 MS.gene069436:CDS
TTACAGAACCTGAACCTTGT+TGG 0.439465 2.2:-30643099 MS.gene069436:CDS
AAGGTTTCTGATCTTGTGAA+TGG 0.443284 2.2:-30642701 MS.gene069436:CDS
TTGATTATGTTGAAGTTGAT+AGG 0.454882 2.2:-30642604 MS.gene069436:CDS
AATGGAGAATGTGTTGATAA+TGG 0.455938 2.2:+30643660 None:intergenic
AAAATCCACCATTGAAAGAA+GGG 0.480777 2.2:-30642763 MS.gene069436:CDS
AAACCGGTTGCAGAAATACT+TGG 0.486758 2.2:-30643502 MS.gene069436:CDS
AAACAGTCACCTGGTTTGAC+AGG 0.489597 2.2:+30643619 None:intergenic
AATGCCACTTGTTATAGCTC+AGG 0.506750 2.2:-30643245 MS.gene069436:CDS
AACCTTAATTCGTAATCTTG+CGG 0.509012 2.2:+30642818 None:intergenic
CCAAAGCAACTTGAGTTTGG+TGG 0.511667 2.2:-30642581 MS.gene069436:CDS
GTGCAACCAGCAGAGTCAAA+TGG 0.513159 2.2:+30642902 None:intergenic
TTCACTTCTGGTGGATCACG+TGG 0.525704 2.2:+30643058 None:intergenic
CCTATTACCCTCTCTCTGGA+AGG 0.526401 2.2:-30643453 MS.gene069436:CDS
TGAAGAACAATACAAAGTGT+TGG 0.527463 2.2:-30643269 MS.gene069436:CDS
TCATTGTATCTGTGATGGAA+TGG 0.531490 2.2:-30643176 MS.gene069436:CDS
TTGCACTACATTTGATGCAA+TGG 0.535105 2.2:-30642885 MS.gene069436:CDS
TAAAACCCTTCTTTCAATGG+TGG 0.535458 2.2:+30642758 None:intergenic
AGAGGGAGTTCTTGAACCCA+TGG 0.536362 2.2:+30643706 None:intergenic
ACATGTGGTGGATTTAGCCT+CGG 0.543992 2.2:-30643211 MS.gene069436:CDS
CGAACCAAAAGAGTAGTCTC+TGG 0.545439 2.2:+30642671 None:intergenic
TGTGGAAATTTCACTTCTGG+TGG 0.551121 2.2:+30643049 None:intergenic
TGCACATATTTGGAGATCAT+GGG 0.553120 2.2:-30642861 MS.gene069436:CDS
CTATGCAGTTTCTAGGAGCA+TGG 0.553170 2.2:-30643150 MS.gene069436:CDS
GTAAACTTACAATTACTCAA+TGG 0.554348 2.2:-30642559 MS.gene069436:CDS
CATTGTATCTGTGATGGAAT+GGG 0.558261 2.2:-30643175 MS.gene069436:CDS
AATACACAAACTTGAGGTGT+AGG 0.562354 2.2:+30642461 None:intergenic
AATAGGCTTTGATGGAGTAA+TGG 0.565700 2.2:+30643642 None:intergenic
TGGGGTAAGCCAATTTATGC+TGG 0.566093 2.2:-30642500 MS.gene069436:CDS
GGAATATTCAAAGGATTGAG+AGG 0.566846 2.2:+30643688 None:intergenic
AAGCCTATTCCTGTCAAACC+AGG 0.569590 2.2:-30643628 MS.gene069436:CDS
AGTTGATCTCAAATATTCCT+CGG 0.570133 2.2:+30642628 None:intergenic
TATGCAGTTTCTAGGAGCAT+GGG 0.573221 2.2:-30643149 MS.gene069436:CDS
AATGTGTTGATAATGGTGAC+TGG 0.575398 2.2:+30643667 None:intergenic
CTCTTAACCTTCCAGAGAGA+GGG 0.576626 2.2:+30643446 None:intergenic
ATGGTGACTGGAATATTCAA+AGG 0.586441 2.2:+30643679 None:intergenic
GAGTTCATGAGAATTGATGA+AGG 0.588836 2.2:-30643022 MS.gene069436:CDS
CTTTGTCATTGTATCTGTGA+TGG 0.588884 2.2:-30643181 MS.gene069436:CDS
GTTGAGCAAGTGATTTCAAG+TGG 0.594391 2.2:+30642924 None:intergenic
TCTCTTAACCTTCCAGAGAG+AGG 0.594993 2.2:+30643445 None:intergenic
GAAAGATATAAACAGTCACC+TGG 0.597824 2.2:+30643610 None:intergenic
AAAATCTCTCTGTTCCAACA+AGG 0.600364 2.2:+30643085 None:intergenic
CAACACTGAATCAATAAGCA+AGG 0.611495 2.2:+30642349 None:intergenic
ATTCTCATGAACTCCATGTG+TGG 0.615728 2.2:+30643031 None:intergenic
AGCGCGTGTCTTCACACCGA+CGG 0.618699 2.2:-30643566 MS.gene069436:CDS
GATTGATAAAAGTAAACCGT+CGG 0.622240 2.2:+30643550 None:intergenic
GTTACCTGAGCTATAACAAG+TGG 0.625798 2.2:+30643241 None:intergenic
AGAAGTGAAATTTCCACACA+TGG 0.632485 2.2:-30643044 MS.gene069436:CDS
GGAGAAGGTGATTCTAGTGG+TGG 0.632681 2.2:-30642428 MS.gene069436:CDS
AACATAGCTTTATCTGAACT+AGG 0.638913 2.2:-30643346 MS.gene069436:CDS
TTCGGAGAAGAAGAAGAACA+AGG 0.639458 2.2:-30643394 MS.gene069436:CDS
GAATATTCAAAGGATTGAGA+GGG 0.647428 2.2:+30643689 None:intergenic
GAAGGAGAAGGTGATTCTAG+TGG 0.654729 2.2:-30642431 MS.gene069436:CDS
CATCACAGATACAATGACAA+AGG 0.659886 2.2:+30643182 None:intergenic
AGCTCGACAAAGTGTAACCG+AGG 0.683483 2.2:-30642645 MS.gene069436:CDS
CCAAAACATCAGCTAGAGCG+CGG 0.685011 2.2:+30643479 None:intergenic
AATGACAAAGGCGTAAACCG+AGG 0.712450 2.2:+30643194 None:intergenic
GGGAGTTCTTGAACCCATGG+AGG 0.731086 2.2:+30643709 None:intergenic
GCGCTCCAATCACGTCATCG+AGG 0.738564 2.2:+30643584 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATAATTGTTCTGATTT+TGG - chr2.2:30643531-30643550 MS.gene069436:CDS 15.0%
!! TTTGGAAATTTGAATATCAA+TGG + chr2.2:30642766-30642785 None:intergenic 20.0%
! ACTTTGTATTGTTCTTCATT+TGG + chr2.2:30642784-30642803 None:intergenic 25.0%
! CAAACTTAACAATGAAACTT+TGG - chr2.2:30643058-30643077 MS.gene069436:CDS 25.0%
! GTAAACTTACAATTACTCAA+TGG - chr2.2:30643496-30643515 MS.gene069436:CDS 25.0%
!! ATAATTGTTCTGATTTTGGA+TGG - chr2.2:30643535-30643554 MS.gene069436:CDS 25.0%
!! TAATTGTTCTGATTTTGGAT+GGG - chr2.2:30643536-30643555 MS.gene069436:CDS 25.0%
!! TTGATTATGTTGAAGTTGAT+AGG - chr2.2:30643451-30643470 MS.gene069436:CDS 25.0%
AAAAATCCACCATTGAAAGA+AGG - chr2.2:30643291-30643310 MS.gene069436:CDS 30.0%
AAAATCCACCATTGAAAGAA+GGG - chr2.2:30643292-30643311 MS.gene069436:CDS 30.0%
AACAGCAAAAACAGGAAAAT+TGG - chr2.2:30642933-30642952 MS.gene069436:CDS 30.0%
AACCTTAATTCGTAATCTTG+CGG + chr2.2:30643240-30643259 None:intergenic 30.0%
AAGTACTTCTTTCCAAAAAC+CGG - chr2.2:30642537-30642556 MS.gene069436:CDS 30.0%
AGTTGATCTCAAATATTCCT+CGG + chr2.2:30643430-30643449 None:intergenic 30.0%
GAATATTCAAAGGATTGAGA+GGG + chr2.2:30642369-30642388 None:intergenic 30.0%
GATTGATAAAAGTAAACCGT+CGG + chr2.2:30642508-30642527 None:intergenic 30.0%
GGTAAAAATACACAAACTTG+AGG + chr2.2:30643603-30643622 None:intergenic 30.0%
TGAAGAACAATACAAAGTGT+TGG - chr2.2:30642786-30642805 MS.gene069436:CDS 30.0%
! AATGGAGAATGTGTTGATAA+TGG + chr2.2:30642398-30642417 None:intergenic 30.0%
! AATTGTTCTGATTTTGGATG+GGG - chr2.2:30643537-30643556 MS.gene069436:CDS 30.0%
!! AACATAGCTTTATCTGAACT+AGG - chr2.2:30642709-30642728 MS.gene069436:CDS 30.0%
!! GTTTGTGTATTTTTACCTGA+AGG - chr2.2:30643606-30643625 MS.gene069436:CDS 30.0%
!!! GGTAAACTGGAAGTATTTTT+CGG - chr2.2:30642643-30642662 MS.gene069436:CDS 30.0%
AAAATCTCTCTGTTCCAACA+AGG + chr2.2:30642973-30642992 None:intergenic 35.0%
AAGGTTTCTGATCTTGTGAA+TGG - chr2.2:30643354-30643373 MS.gene069436:CDS 35.0%
AATACACAAACTTGAGGTGT+AGG + chr2.2:30643597-30643616 None:intergenic 35.0%
AATATCAATGGTTCCCAAGA+TGG + chr2.2:30642754-30642773 None:intergenic 35.0%
AGAAGAACAAGGTGCATTAA+TGG - chr2.2:30642672-30642691 MS.gene069436:CDS 35.0%
AGAAGTGAAATTTCCACACA+TGG - chr2.2:30643011-30643030 MS.gene069436:CDS 35.0%
AGGTTAAGAGAGACGAAAAA+CGG - chr2.2:30642622-30642641 MS.gene069436:CDS 35.0%
ATGGTGACTGGAATATTCAA+AGG + chr2.2:30642379-30642398 None:intergenic 35.0%
ATGTGTGGAAATTTCACTTC+TGG + chr2.2:30643012-30643031 None:intergenic 35.0%
CAACACTGAATCAATAAGCA+AGG + chr2.2:30643709-30643728 None:intergenic 35.0%
CATCACAGATACAATGACAA+AGG + chr2.2:30642876-30642895 None:intergenic 35.0%
CCATAAAACCCTTCTTTCAA+TGG + chr2.2:30643303-30643322 None:intergenic 35.0%
CTTTGTCATTGTATCTGTGA+TGG - chr2.2:30642874-30642893 MS.gene069436:CDS 35.0%
GAAAGATATAAACAGTCACC+TGG + chr2.2:30642448-30642467 None:intergenic 35.0%
GAGTTCATGAGAATTGATGA+AGG - chr2.2:30643033-30643052 MS.gene069436:CDS 35.0%
GGAATATTCAAAGGATTGAG+AGG + chr2.2:30642370-30642389 None:intergenic 35.0%
GGTGTAGGTGTTAAATCAAT+AGG + chr2.2:30643582-30643601 None:intergenic 35.0%
TAAAACCCTTCTTTCAATGG+TGG + chr2.2:30643300-30643319 None:intergenic 35.0%
TATTGCTTGCACAACAAGTA+AGG - chr2.2:30643335-30643354 MS.gene069436:CDS 35.0%
TCAATAGGACCAGCATAAAT+TGG + chr2.2:30643567-30643586 None:intergenic 35.0%
TGCACATATTTGGAGATCAT+GGG - chr2.2:30643194-30643213 MS.gene069436:CDS 35.0%
TTGCACTACATTTGATGCAA+TGG - chr2.2:30643170-30643189 MS.gene069436:CDS 35.0%
! AATAGGCTTTGATGGAGTAA+TGG + chr2.2:30642416-30642435 None:intergenic 35.0%
! ATTACCAGAGACTACTCTTT+TGG - chr2.2:30643380-30643399 MS.gene069436:CDS 35.0%
! CAGGTAACAATTTTCACATG+TGG - chr2.2:30642829-30642848 MS.gene069436:CDS 35.0%
! CATTGTATCTGTGATGGAAT+GGG - chr2.2:30642880-30642899 MS.gene069436:CDS 35.0%
! CCATTGAAAGAAGGGTTTTA+TGG - chr2.2:30643300-30643319 MS.gene069436:CDS 35.0%
! GTAACAATTTTCACATGTGG+TGG - chr2.2:30642832-30642851 MS.gene069436:CDS 35.0%
! TCATTGTATCTGTGATGGAA+TGG - chr2.2:30642879-30642898 MS.gene069436:CDS 35.0%
!! AATGTGTTGATAATGGTGAC+TGG + chr2.2:30642391-30642410 None:intergenic 35.0%
!! AGAAGGGTTTTATGGTAATG+TGG - chr2.2:30643308-30643327 MS.gene069436:CDS 35.0%
!! GTATTTTTACCTGAAGGAGA+AGG - chr2.2:30643612-30643631 MS.gene069436:CDS 35.0%
AAACCGGTTGCAGAAATACT+TGG - chr2.2:30642553-30642572 MS.gene069436:CDS 40.0%
AGAGACGAAAAACGGTAAAC+TGG - chr2.2:30642630-30642649 MS.gene069436:CDS 40.0%
AGCCGCAAGATTACGAATTA+AGG - chr2.2:30643235-30643254 MS.gene069436:CDS 40.0%
ATGCAATGGCTGCACATATT+TGG - chr2.2:30643184-30643203 MS.gene069436:CDS 40.0%
ATGCGATAACACTTCTGAAC+AGG + chr2.2:30643093-30643112 None:intergenic 40.0%
ATTCTCATGAACTCCATGTG+TGG + chr2.2:30643027-30643046 None:intergenic 40.0%
CTGCACATATTTGGAGATCA+TGG - chr2.2:30643193-30643212 MS.gene069436:CDS 40.0%
GTTACCTGAGCTATAACAAG+TGG + chr2.2:30642817-30642836 None:intergenic 40.0%
GTTGAGCAAGTGATTTCAAG+TGG + chr2.2:30643134-30643153 None:intergenic 40.0%
TGTGGAAATTTCACTTCTGG+TGG + chr2.2:30643009-30643028 None:intergenic 40.0%
TTACAGAACCTGAACCTTGT+TGG - chr2.2:30642956-30642975 MS.gene069436:CDS 40.0%
TTCGGAGAAGAAGAAGAACA+AGG - chr2.2:30642661-30642680 MS.gene069436:CDS 40.0%
! AACTTGTTCGCAGCAAATTC+AGG + chr2.2:30643678-30643697 None:intergenic 40.0%
! AATGGTTCCCAAGATGGATT+TGG + chr2.2:30642748-30642767 None:intergenic 40.0%
! TATGCAGTTTCTAGGAGCAT+GGG - chr2.2:30642906-30642925 MS.gene069436:CDS 40.0%
! TTGACAGGAATAGGCTTTGA+TGG + chr2.2:30642424-30642443 None:intergenic 40.0%
!! AATGCCACTTGTTATAGCTC+AGG - chr2.2:30642810-30642829 MS.gene069436:CDS 40.0%
!! GTATTTCTGCAACCGGTTTT+TGG + chr2.2:30642552-30642571 None:intergenic 40.0%
AAGCCTATTCCTGTCAAACC+AGG - chr2.2:30642427-30642446 MS.gene069436:CDS 45.0%
AGGCCAAAGCAACTTGAGTT+TGG - chr2.2:30643471-30643490 MS.gene069436:CDS 45.0%
ATGGGAGCTATGCAGTTTCT+AGG - chr2.2:30642898-30642917 MS.gene069436:CDS 45.0%
CCAAAGCAACTTGAGTTTGG+TGG - chr2.2:30643474-30643493 MS.gene069436:CDS 45.0%
CGAACCAAAAGAGTAGTCTC+TGG + chr2.2:30643387-30643406 None:intergenic 45.0%
CTAGAATCACCTTCTCCTTC+AGG + chr2.2:30643624-30643643 None:intergenic 45.0%
CTCTTAACCTTCCAGAGAGA+GGG + chr2.2:30642612-30642631 None:intergenic 45.0%
CTTCCAGAGAGAGGGTAATA+GGG + chr2.2:30642604-30642623 None:intergenic 45.0%
GAAGGAGAAGGTGATTCTAG+TGG - chr2.2:30643624-30643643 MS.gene069436:CDS 45.0%
TCAAACCTCGATGACGTGAT+TGG - chr2.2:30642466-30642485 MS.gene069436:CDS 45.0%
TCTCTGTTCCAACAAGGTTC+AGG + chr2.2:30642967-30642986 None:intergenic 45.0%
TCTCTTAACCTTCCAGAGAG+AGG + chr2.2:30642613-30642632 None:intergenic 45.0%
TGCTGCTCCAAATCCATCTT+GGG - chr2.2:30642738-30642757 MS.gene069436:CDS 45.0%
TGGGGTAAGCCAATTTATGC+TGG - chr2.2:30643555-30643574 MS.gene069436:CDS 45.0%
TTGCTGCTCCAAATCCATCT+TGG - chr2.2:30642737-30642756 MS.gene069436:CDS 45.0%
! AAACAGTCACCTGGTTTGAC+AGG + chr2.2:30642439-30642458 None:intergenic 45.0%
! AATGACAAAGGCGTAAACCG+AGG + chr2.2:30642864-30642883 None:intergenic 45.0%
! CCACCAAACTCAAGTTGCTT+TGG + chr2.2:30643477-30643496 None:intergenic 45.0%
! CTATGCAGTTTCTAGGAGCA+TGG - chr2.2:30642905-30642924 MS.gene069436:CDS 45.0%
! TCACCTGGTTTGACAGGAAT+AGG + chr2.2:30642433-30642452 None:intergenic 45.0%
!! ACATGTGGTGGATTTAGCCT+CGG - chr2.2:30642844-30642863 MS.gene069436:CDS 45.0%
AGAGGGAGTTCTTGAACCCA+TGG + chr2.2:30642352-30642371 None:intergenic 50.0%
AGCTCGACAAAGTGTAACCG+AGG - chr2.2:30643410-30643429 MS.gene069436:CDS 50.0%
CCAAAACATCAGCTAGAGCG+CGG + chr2.2:30642579-30642598 None:intergenic 50.0%
CCTATTACCCTCTCTCTGGA+AGG - chr2.2:30642602-30642621 MS.gene069436:CDS 50.0%
CCTTCCAGAGAGAGGGTAAT+AGG + chr2.2:30642605-30642624 None:intergenic 50.0%
GCTCAACCATTTGACTCTGC+TGG - chr2.2:30643147-30643166 MS.gene069436:CDS 50.0%
GGAGAAGGTGATTCTAGTGG+TGG - chr2.2:30643627-30643646 MS.gene069436:CDS 50.0%
GTGCAACCAGCAGAGTCAAA+TGG + chr2.2:30643156-30643175 None:intergenic 50.0%
GTTCCCTATTACCCTCTCTC+TGG - chr2.2:30642598-30642617 MS.gene069436:CDS 50.0%
TGGGCTGCAACAGCAAAAAC+AGG - chr2.2:30642925-30642944 MS.gene069436:CDS 50.0%
! CGGCCAAGTATTTCTGCAAC+CGG + chr2.2:30642559-30642578 None:intergenic 50.0%
! TTCACTTCTGGTGGATCACG+TGG + chr2.2:30643000-30643019 None:intergenic 50.0%
GGGAGTTCTTGAACCCATGG+AGG + chr2.2:30642349-30642368 None:intergenic 55.0%
! CCGCGCTCTAGCTGATGTTT+TGG - chr2.2:30642576-30642595 MS.gene069436:CDS 55.0%
AGCGCGTGTCTTCACACCGA+CGG - chr2.2:30642489-30642508 MS.gene069436:CDS 60.0%
GCGCTCCAATCACGTCATCG+AGG + chr2.2:30642474-30642493 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 30642340 30643737 30642340 ID=MS.gene069436
chr2.2 mRNA 30642340 30643737 30642340 ID=MS.gene069436.t1;Parent=MS.gene069436
chr2.2 exon 30642340 30643737 30642340 ID=MS.gene069436.t1.exon1;Parent=MS.gene069436.t1
chr2.2 CDS 30642340 30643737 30642340 ID=cds.MS.gene069436.t1;Parent=MS.gene069436.t1
Gene Sequence

>MS.gene069436

ATGGCTCCTCCATGGGTTCAAGAACTCCCTCTCAATCCTTTGAATATTCCAGTCACCATTATCAACACATTCTCCATTACTCCATCAAAGCCTATTCCTGTCAAACCAGGTGACTGTTTATATCTTTCAAACCTCGATGACGTGATTGGAGCGCGTGTCTTCACACCGACGGTTTACTTTTATCAATCAGATAATTCAAGTACTTCTTTCCAAAAACCGGTTGCAGAAATACTTGGCCGCGCTCTAGCTGATGTTTTGGTTCCCTATTACCCTCTCTCTGGAAGGTTAAGAGAGACGAAAAACGGTAAACTGGAAGTATTTTTCGGAGAAGAAGAAGAACAAGGTGCATTAATGGTTGAAGCAAGATCAAACATAGCTTTATCTGAACTAGGTGATTTTGCTGCTCCAAATCCATCTTGGGAACCATTGATATTCAAATTTCCAAATGAAGAACAATACAAAGTGTTGGAAATGCCACTTGTTATAGCTCAGGTAACAATTTTCACATGTGGTGGATTTAGCCTCGGTTTACGCCTTTGTCATTGTATCTGTGATGGAATGGGAGCTATGCAGTTTCTAGGAGCATGGGCTGCAACAGCAAAAACAGGAAAATTGGTTACAGAACCTGAACCTTGTTGGAACAGAGAGATTTTCAAGCCACGTGATCCACCAGAAGTGAAATTTCCACACATGGAGTTCATGAGAATTGATGAAGGATCAAACTTAACAATGAAACTTTGGAAAACAAAGCCTGTTCAGAAGTGTTATCGCATAAGACGCGAGTTTCAAAACCACTTGAAATCACTTGCTCAACCATTTGACTCTGCTGGTTGCACTACATTTGATGCAATGGCTGCACATATTTGGAGATCATGGGTTAAAGCTCTTGATGTTAAGCCGCAAGATTACGAATTAAGGTTAACATTTTCAGTTAATGCTAGACAGAAGCTTAAAAATCCACCATTGAAAGAAGGGTTTTATGGTAATGTGGTTTGTATTGCTTGCACAACAAGTAAGGTTTCTGATCTTGTGAATGGAAAATTACCAGAGACTACTCTTTTGGTTCGTGAAGCTCGACAAAGTGTAACCGAGGAATATTTGAGATCAACTGTTGATTATGTTGAAGTTGATAGGCCAAAGCAACTTGAGTTTGGTGGTAAACTTACAATTACTCAATGGACAAGATTTTCTATTTATAATTGTTCTGATTTTGGATGGGGTAAGCCAATTTATGCTGGTCCTATTGATTTAACACCTACACCTCAAGTTTGTGTATTTTTACCTGAAGGAGAAGGTGATTCTAGTGGTGGTTCTATGATTGTTTGCATTTGCTTGCCTGAATTTGCTGCGAACAAGTTTACACAAGCCTTGCTTATTGATTCAGTGTTGTTGACATAG
Protein sequence

>MS.gene069436.t1

MAPPWVQELPLNPLNIPVTIINTFSITPSKPIPVKPGDCLYLSNLDDVIGARVFTPTVYFYQSDNSSTSFQKPVAEILGRALADVLVPYYPLSGRLRETKNGKLEVFFGEEEEQGALMVEARSNIALSELGDFAAPNPSWEPLIFKFPNEEQYKVLEMPLVIAQVTIFTCGGFSLGLRLCHCICDGMGAMQFLGAWAATAKTGKLVTEPEPCWNREIFKPRDPPEVKFPHMEFMRIDEGSNLTMKLWKTKPVQKCYRIRREFQNHLKSLAQPFDSAGCTTFDAMAAHIWRSWVKALDVKPQDYELRLTFSVNARQKLKNPPLKEGFYGNVVCIACTTSKVSDLVNGKLPETTLLVREARQSVTEEYLRSTVDYVEVDRPKQLEFGGKLTITQWTRFSIYNCSDFGWGKPIYAGPIDLTPTPQVCVFLPEGEGDSSGGSMIVCICLPEFAANKFTQALLIDSVLLT