AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042317

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042317.t1 MTR_1g053315 99.140 465 4 0 1 465 1 465 0.0 954
MS.gene042317.t1 MTR_3g098980 35.477 451 262 16 18 460 6 435 5.96e-83 263
MS.gene042317.t1 MTR_7g027175 35.354 396 232 15 41 431 36 412 8.24e-71 231
MS.gene042317.t1 MTR_8g096530 33.925 451 260 16 22 459 3 428 7.67e-68 224
MS.gene042317.t1 MTR_3g112180 35.000 440 245 17 25 453 19 428 8.27e-64 213
MS.gene042317.t1 MTR_5g076700 30.664 437 285 12 31 463 20 442 2.98e-59 201
MS.gene042317.t1 MTR_4g007540 31.416 452 273 18 20 457 2 430 9.10e-58 197
MS.gene042317.t1 MTR_8g075610 31.849 449 261 20 26 460 2 419 1.38e-57 196
MS.gene042317.t1 MTR_2g064495 30.599 451 273 18 20 455 2 427 5.45e-57 194
MS.gene042317.t1 MTR_2g105330 30.377 451 274 18 20 455 2 427 1.49e-55 191
MS.gene042317.t1 MTR_1g081660 30.324 432 281 13 31 457 28 444 6.45e-55 190
MS.gene042317.t1 MTR_7g114470 29.864 442 290 12 26 463 19 444 9.01e-55 189
MS.gene042317.t1 MTR_7g114490 31.591 440 277 14 24 453 8 433 1.89e-54 190
MS.gene042317.t1 MTR_3g049150 30.067 449 270 16 18 443 2 429 4.01e-54 187
MS.gene042317.t1 MTR_7g114520 29.977 437 286 13 26 457 19 440 5.63e-54 187
MS.gene042317.t1 MTR_7g114560 30.023 443 286 15 26 463 18 441 1.41e-51 181
MS.gene042317.t1 MTR_7g114550 30.856 444 281 16 26 463 18 441 2.82e-51 180
MS.gene042317.t1 MTR_1g040175 31.362 389 251 10 26 413 17 390 1.52e-50 178
MS.gene042317.t1 MTR_7g084940 28.953 449 286 14 14 453 12 436 5.27e-50 176
MS.gene042317.t1 MTR_1g040215 31.378 392 249 11 26 414 17 391 1.82e-49 175
MS.gene042317.t1 MTR_4g055170 29.464 448 285 17 26 457 20 452 5.99e-49 174
MS.gene042317.t1 MTR_3g071320 32.321 461 258 23 18 457 5 432 7.43e-49 173
MS.gene042317.t1 MTR_4g127650 29.764 467 283 16 18 460 10 455 6.13e-48 171
MS.gene042317.t1 MTR_3g026950 28.132 455 275 16 18 443 2 433 7.11e-48 171
MS.gene042317.t1 MTR_1g040195 29.120 443 265 14 26 463 17 415 2.15e-47 169
MS.gene042317.t1 MTR_4g097090 30.090 442 261 14 47 460 34 455 6.01e-47 169
MS.gene042317.t1 MTR_7g085990 29.032 465 275 15 32 460 11 456 2.16e-46 167
MS.gene042317.t1 MTR_7g085930 28.936 470 269 16 32 460 15 460 5.12e-46 166
MS.gene042317.t1 MTR_2g015430 29.385 439 276 18 33 457 16 434 1.57e-45 164
MS.gene042317.t1 MTR_1g040320 27.891 441 262 13 26 463 17 404 9.55e-45 162
MS.gene042317.t1 MTR_7g084910 29.613 439 262 17 15 443 14 415 1.85e-41 153
MS.gene042317.t1 MTR_7g056087 26.535 456 297 18 18 458 4 436 7.19e-41 152
MS.gene042317.t1 MTR_3g465930 28.665 457 280 17 30 457 19 458 3.96e-39 147
MS.gene042317.t1 MTR_1g040315 27.356 435 254 14 26 457 17 392 2.08e-37 142
MS.gene042317.t1 MTR_3g465990 27.766 461 280 19 26 457 18 454 3.53e-37 142
MS.gene042317.t1 MTR_3g465980 27.983 461 279 19 26 457 18 454 8.25e-37 141
MS.gene042317.t1 MTR_4g013050 28.000 450 241 21 19 445 5 394 8.59e-37 140
MS.gene042317.t1 MTR_3g465910 29.148 446 278 19 34 453 18 451 4.13e-35 137
MS.gene042317.t1 MTR_7g086000 28.200 461 279 18 16 445 2 441 4.49e-34 133
MS.gene042317.t1 MTR_6g053810 28.348 448 275 21 39 457 29 459 1.05e-33 132
MS.gene042317.t1 MTR_7g114510 29.450 309 198 10 158 457 1 298 5.98e-32 124
MS.gene042317.t1 MTR_3g465950 28.009 457 278 20 26 457 18 448 2.31e-31 125
MS.gene042317.t1 MTR_3g465880 28.602 472 293 25 13 457 7 461 1.31e-30 124
MS.gene042317.t1 MTR_7g110610 27.470 415 222 14 23 424 2 350 4.13e-30 121
MS.gene042317.t1 MTR_0502s0010 30.738 244 157 6 117 357 1 235 3.30e-28 112
MS.gene042317.t1 MTR_5g090830 25.123 406 251 15 40 412 29 414 8.59e-25 107
MS.gene042317.t1 MTR_5g026320 24.706 425 287 14 35 447 34 437 9.07e-25 107
MS.gene042317.t1 MTR_5g090810 25.436 401 256 14 40 412 29 414 1.14e-24 106
MS.gene042317.t1 MTR_7g114420 28.014 282 166 10 185 463 3 250 1.53e-23 100
MS.gene042317.t1 MTR_3g465870 30.492 305 175 14 14 301 8 292 2.05e-23 102
MS.gene042317.t1 MTR_5g090880 25.479 365 223 14 78 410 67 414 2.53e-22 99.8
MS.gene042317.t1 MTR_3g086020 24.762 420 255 17 14 418 1 374 2.56e-22 99.4
MS.gene042317.t1 MTR_0042s0190 27.706 231 162 4 183 413 1 226 3.57e-22 96.7
MS.gene042317.t1 MTR_3g450710 25.849 383 241 15 58 412 46 413 5.03e-21 95.9
MS.gene042317.t1 MTR_8g079980 25.057 439 264 21 20 433 5 403 1.69e-20 94.0
MS.gene042317.t1 MTR_3g450690 25.762 361 227 14 78 412 72 417 3.25e-20 93.6
MS.gene042317.t1 MTR_8g079970 24.764 424 252 14 16 412 1 384 3.07e-19 90.1
MS.gene042317.t1 MTR_4g094838 24.528 371 229 14 58 410 45 382 8.06e-19 89.0
MS.gene042317.t1 MTR_0190s0020 24.806 387 248 18 78 446 63 424 1.98e-18 87.8
MS.gene042317.t1 MTR_8g467580 24.832 447 271 22 14 432 1 410 2.05e-18 87.8
MS.gene042317.t1 MTR_4g022825 23.571 420 258 18 60 446 33 422 6.20e-18 86.3
MS.gene042317.t1 MTR_4g094835 24.581 358 220 17 78 410 64 396 6.26e-17 83.2
MS.gene042317.t1 MTR_3g049140 28.505 214 128 8 248 443 20 226 8.55e-17 80.5
MS.gene042317.t1 MTR_8g041770 24.331 411 267 19 21 410 4 391 1.57e-16 82.0
MS.gene042317.t1 MTR_4g094842 23.563 348 240 14 78 410 62 398 4.32e-16 80.5
MS.gene042317.t1 MTR_8g467590 24.201 438 274 22 22 432 4 410 5.02e-16 80.5
MS.gene042317.t1 MTR_0042s0190 28.378 148 104 2 266 413 16 161 1.81e-15 76.3
MS.gene042317.t1 MTR_3g088420 25.193 389 245 21 78 447 55 416 2.32e-15 78.2
MS.gene042317.t1 MTR_6g045553 23.146 445 280 20 51 455 44 466 3.15e-15 78.2
MS.gene042317.t1 MTR_4g094832 24.522 314 197 16 115 410 89 380 4.80e-15 77.4
MS.gene042317.t1 MTR_7g114500 32.857 140 83 5 26 164 18 147 1.43e-14 71.6
MS.gene042317.t1 MTR_2g007880 22.248 427 272 17 16 416 15 407 3.08e-14 75.1
MS.gene042317.t1 MTR_3g101960 24.242 462 277 23 21 445 6 431 3.44e-14 74.7
MS.gene042317.t1 MTR_4g022720 23.944 355 226 15 78 410 55 387 3.66e-14 74.7
MS.gene042317.t1 MTR_4g022670 25.634 355 226 17 78 410 55 393 8.02e-14 73.6
MS.gene042317.t1 MTR_6g015320 25.168 298 194 13 160 437 143 431 1.60e-13 72.8
MS.gene042317.t1 MTR_0042s0230 34.965 143 82 6 26 167 17 149 2.34e-13 68.2
MS.gene042317.t1 MTR_7g014200 22.539 386 254 14 58 409 46 420 2.71e-13 72.0
MS.gene042317.t1 MTR_8g039950 39.640 111 59 4 26 136 17 119 6.79e-13 65.9
MS.gene042317.t1 MTR_1g046770 30.000 180 96 8 279 457 36 186 1.26e-12 67.0
MS.gene042317.t1 MTR_7g114960 22.016 377 252 11 18 381 8 355 1.27e-12 70.1
MS.gene042317.t1 MTR_3g026310 23.492 315 211 11 151 447 129 431 1.59e-12 69.7
MS.gene042317.t1 MTR_7g085960 28.079 203 107 8 279 465 15 194 2.92e-12 65.9
MS.gene042317.t1 MTR_1g094790 23.128 454 269 20 18 432 3 415 5.75e-12 67.8
MS.gene042317.t1 MTR_4g022800 22.905 358 233 15 78 410 55 394 5.76e-12 67.8
MS.gene042317.t1 MTR_7g014160 23.214 392 249 17 58 409 46 425 1.37e-11 66.6
MS.gene042317.t1 MTR_3g049080 33.333 87 52 2 18 104 2 82 8.94e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042317.t1 AT1G03390 66.304 460 144 6 6 460 5 458 0.0 625
MS.gene042317.t1 AT1G27620 36.384 437 240 18 31 458 19 426 1.28e-73 239
MS.gene042317.t1 AT5G41040 34.382 445 262 15 24 458 15 439 3.35e-73 238
MS.gene042317.t1 AT5G41040 34.382 445 262 15 24 458 31 455 4.08e-73 238
MS.gene042317.t1 AT3G48720 32.407 432 269 14 31 459 19 430 2.68e-67 222
MS.gene042317.t1 AT5G63560 33.562 438 255 18 26 453 9 420 1.12e-65 218
MS.gene042317.t1 AT3G03480 33.560 441 262 16 26 457 27 445 4.28e-58 198
MS.gene042317.t1 AT5G17540 32.960 446 259 17 26 457 17 436 9.22e-56 192
MS.gene042317.t1 AT5G48930 30.580 448 280 15 21 457 3 430 3.06e-55 190
MS.gene042317.t1 AT1G28680 30.464 453 286 19 18 457 4 440 2.42e-52 183
MS.gene042317.t1 AT2G40230 30.820 451 267 18 19 453 3 424 1.51e-51 180
MS.gene042317.t1 AT1G65450 28.390 472 292 18 1 455 1 443 5.23e-45 163
MS.gene042317.t1 AT2G19070 29.263 475 278 20 17 460 3 450 2.16e-44 161
MS.gene042317.t1 AT1G28680 30.831 373 238 15 26 392 12 370 5.79e-44 159
MS.gene042317.t1 AT5G57840 28.200 461 274 15 21 455 3 432 1.10e-39 149
MS.gene042317.t1 AT3G62160 30.077 389 233 15 36 415 20 378 6.86e-39 146
MS.gene042317.t1 AT5G07080 26.609 466 281 13 20 457 13 445 1.09e-38 146
MS.gene042317.t1 AT4G31910 28.281 442 275 19 42 458 32 456 3.52e-35 136
MS.gene042317.t1 AT2G23510 29.605 456 274 21 23 457 18 447 9.31e-35 135
MS.gene042317.t1 AT2G25150 27.556 450 288 19 30 457 16 449 1.37e-31 126
MS.gene042317.t1 AT1G24430 23.624 436 279 18 14 433 1 398 3.32e-25 107
MS.gene042317.t1 AT3G47170 25.155 485 278 22 26 463 20 466 6.81e-25 107
MS.gene042317.t1 AT3G26040 26.214 412 257 16 18 413 3 383 1.19e-24 106
MS.gene042317.t1 AT1G65450 26.621 293 189 11 175 455 1 279 1.87e-22 97.4
MS.gene042317.t1 AT5G67160 28.612 353 233 13 70 414 48 389 6.07e-22 98.2
MS.gene042317.t1 AT1G31490 23.134 402 275 16 18 409 6 383 1.93e-20 94.0
MS.gene042317.t1 AT1G78990 24.309 362 234 13 70 412 58 398 7.08e-19 89.4
MS.gene042317.t1 AT5G23940 23.641 423 271 18 16 409 1 400 2.30e-18 87.8
MS.gene042317.t1 AT1G32910 24.533 375 226 15 70 412 52 401 4.99e-18 86.7
MS.gene042317.t1 AT3G50280 25.281 356 232 13 78 414 59 399 7.49e-18 86.3
MS.gene042317.t1 AT3G29590 24.816 407 244 16 86 459 69 446 1.60e-17 85.1
MS.gene042317.t1 AT5G38130 25.474 369 238 18 70 416 66 419 5.90e-17 83.6
MS.gene042317.t1 AT5G16410 24.173 393 249 17 70 438 79 446 2.77e-16 81.6
MS.gene042317.t1 AT3G50300 24.866 374 238 15 67 414 43 399 1.11e-15 79.3
MS.gene042317.t1 AT3G30280 24.324 407 249 19 30 411 15 387 1.45e-15 79.0
MS.gene042317.t1 AT5G47980 23.898 431 253 15 14 413 1 387 1.57e-15 79.0
MS.gene042317.t1 AT5G23970 24.396 455 272 20 34 464 11 417 3.69e-15 77.8
MS.gene042317.t1 AT5G07870 24.638 483 297 23 18 465 8 458 1.31e-14 76.3
MS.gene042317.t1 AT3G29670 23.628 419 265 21 58 448 45 436 2.74e-14 75.1
MS.gene042317.t1 AT4G15390 21.957 460 314 17 11 455 1 430 2.53e-13 72.0
MS.gene042317.t1 AT4G15390 21.957 460 314 17 11 455 1 430 2.53e-13 72.0
MS.gene042317.t1 AT3G50280 26.182 275 172 11 156 414 10 269 6.86e-13 69.7
MS.gene042317.t1 AT1G24420 23.114 411 266 20 18 412 5 381 1.36e-12 69.7
MS.gene042317.t1 AT1G24420 23.114 411 266 20 18 412 5 381 1.36e-12 69.7
MS.gene042317.t1 AT5G47950 29.557 203 123 10 30 229 15 200 3.32e-12 68.6
MS.gene042317.t1 AT5G61160 22.812 377 263 15 55 412 38 405 1.76e-11 66.6
MS.gene042317.t1 AT5G39090 22.296 453 300 16 14 440 1 427 5.52e-11 64.7

Find 92 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTATAATTGTTCTGATTT+TGG 0.098860 2.4:-36292156 MS.gene042317:CDS
AGCCGCAAGATTACGAATTA+AGG 0.212133 2.4:-36292452 MS.gene042317:CDS
ATGCAATGGCTGCACATATT+TGG 0.230199 2.4:-36292503 MS.gene042317:CDS
CCTTCCAGAGAGAGGGTAAT+AGG 0.259302 2.4:+36293085 None:intergenic
AACTTGTTCGCAGCAGATTC+AGG 0.294220 2.4:+36292012 None:intergenic
CCGCGCTCTAGCTGATGTTT+TGG 0.307482 2.4:-36293111 MS.gene042317:CDS
TCAATAGGACCAGCATAAAT+TGG 0.316153 2.4:+36292123 None:intergenic
AGAAGAACAAGGAGCGTTAA+TGG 0.317914 2.4:-36293015 MS.gene042317:CDS
ATTACCAGAGACTACTCTTT+TGG 0.326454 2.4:-36292307 MS.gene042317:CDS
ACTTTGTATTGTTCTTCATT+TGG 0.336455 2.4:+36292906 None:intergenic
TTGACAGGAATAGGCTTTGA+TGG 0.354952 2.4:+36293266 None:intergenic
TCTCTGTTCCAACAAGGTTC+AGG 0.357662 2.4:+36292723 None:intergenic
AATGGTTCCCAAGATGGATT+TGG 0.362060 2.4:+36292942 None:intergenic
CCATAAAACCCTTCTTTCAA+TGG 0.366018 2.4:+36292387 None:intergenic
ATGGGAGCTATGCAGTTTCT+AGG 0.367373 2.4:-36292789 MS.gene042317:CDS
TGCTGCTCCAAATCCATCTT+GGG 0.372169 2.4:-36292949 MS.gene042317:CDS
CCACCAAACTCAAGTTGCTT+TGG 0.374913 2.4:+36292213 None:intergenic
CTAGAATCACCTTCTCCTTC+AGG 0.380164 2.4:+36292066 None:intergenic
AGGCCAAAGCAACTTGAGTT+TGG 0.383129 2.4:-36292216 MS.gene042317:CDS
TCAAACCTCGATGATGTGAT+TGG 0.383794 2.4:-36293221 MS.gene042317:CDS
ATATGTGGAAATTTCACTTC+TGG 0.395042 2.4:+36292678 None:intergenic
TTGCTGCTCCAAATCCATCT+TGG 0.397296 2.4:-36292950 MS.gene042317:CDS
CAAACTTAACAATGAAACTT+TGG 0.400300 2.4:-36292629 MS.gene042317:CDS
TTTGGAAATTTGAATATCAA+TGG 0.400500 2.4:+36292924 None:intergenic
CTTCCAGAGAGAGGGTAATA+GGG 0.404106 2.4:+36293086 None:intergenic
ATGCGATAACACTTCTGAAC+AGG 0.407661 2.4:+36292597 None:intergenic
AATATCAATGGTTCCCAAGA+TGG 0.408999 2.4:+36292936 None:intergenic
AGAAGTGAAATTTCCACATA+TGG 0.416239 2.4:-36292676 MS.gene042317:CDS
TCACCTGGTTTGACAGGAAT+AGG 0.417319 2.4:+36293257 None:intergenic
GGTGTAGGTGTTAAATCAAT+AGG 0.417668 2.4:+36292108 None:intergenic
CGGCCAAGTATTTCTGCAAC+CGG 0.424613 2.4:+36293131 None:intergenic
CTGCACATATTTGGAGATCA+TGG 0.426583 2.4:-36292494 MS.gene042317:CDS
GCTCAACCATTTGATTCTGC+TGG 0.426988 2.4:-36292540 MS.gene042317:CDS
GTTCCCTATTACCCTCTCTC+TGG 0.430323 2.4:-36293089 MS.gene042317:CDS
TATTGCTTGCACAACAAGTA+AGG 0.435787 2.4:-36292352 MS.gene042317:CDS
TTACAGAACCTGAACCTTGT+TGG 0.439465 2.4:-36292731 MS.gene042317:CDS
AAGGTTTCTGATCTTGTGAA+TGG 0.443284 2.4:-36292333 MS.gene042317:CDS
TTGATTATGTTGAAGTTGAT+AGG 0.454882 2.4:-36292236 MS.gene042317:CDS
AAAGAGTCACCTGGTTTGAC+AGG 0.465860 2.4:+36293251 None:intergenic
AACGGAGAATGTGTTGATAA+TGG 0.468370 2.4:+36293292 None:intergenic
AAAATCCACCATTGAAAGAA+GGG 0.480777 2.4:-36292395 MS.gene042317:CDS
AAACCGGTTGCAGAAATACT+TGG 0.486758 2.4:-36293134 MS.gene042317:CDS
AATGCCACTTGTTATAGCTC+AGG 0.506750 2.4:-36292877 MS.gene042317:CDS
AACCTTAATTCGTAATCTTG+CGG 0.509012 2.4:+36292450 None:intergenic
GAACAAGTTTACACAAGCCT+TGG 0.510546 2.4:-36291998 MS.gene042317:CDS
CCAAAGCAACTTGAGTTTGG+TGG 0.511667 2.4:-36292213 MS.gene042317:CDS
TGTGGAAATTTCACTTCTGG+TGG 0.512819 2.4:+36292681 None:intergenic
GTGCAACCAGCAGAATCAAA+TGG 0.517769 2.4:+36292534 None:intergenic
TTCACTTCTGGTGGATCACG+TGG 0.525704 2.4:+36292690 None:intergenic
CCTATTACCCTCTCTCTGGA+AGG 0.526401 2.4:-36293085 MS.gene042317:CDS
TGAAGAACAATACAAAGTGT+TGG 0.527463 2.4:-36292901 MS.gene042317:CDS
TCATTGTATCTGTGATGGAA+TGG 0.531490 2.4:-36292808 MS.gene042317:CDS
TTGCACTACATTTGATGCAA+TGG 0.535105 2.4:-36292517 MS.gene042317:CDS
TAAAACCCTTCTTTCAATGG+TGG 0.535458 2.4:+36292390 None:intergenic
AGAGGGAGTTCTTGAACCCA+TGG 0.536362 2.4:+36293338 None:intergenic
ACATGTGGTGGATTTAGCCT+CGG 0.543992 2.4:-36292843 MS.gene042317:CDS
CGAACCAAAAGAGTAGTCTC+TGG 0.545439 2.4:+36292303 None:intergenic
ATTCTCATGAACTCCATATG+TGG 0.547153 2.4:+36292663 None:intergenic
TGCACATATTTGGAGATCAT+GGG 0.553120 2.4:-36292493 MS.gene042317:CDS
CTATGCAGTTTCTAGGAGCA+TGG 0.553170 2.4:-36292782 MS.gene042317:CDS
GTAAACTTACAATTACTCAA+TGG 0.554348 2.4:-36292191 MS.gene042317:CDS
CATTGTATCTGTGATGGAAT+GGG 0.558261 2.4:-36292807 MS.gene042317:CDS
AATACACAAACTTGAGGTGT+AGG 0.562354 2.4:+36292093 None:intergenic
TGGGGTAAGCCAATTTATGC+TGG 0.566093 2.4:-36292132 MS.gene042317:CDS
GGAATATTCAAAGGATTGAG+AGG 0.566846 2.4:+36293320 None:intergenic
AAGCCTATTCCTGTCAAACC+AGG 0.569590 2.4:-36293260 MS.gene042317:CDS
AGTTGATCTCAAATATTCCT+CGG 0.570133 2.4:+36292260 None:intergenic
TATGCAGTTTCTAGGAGCAT+GGG 0.573221 2.4:-36292781 MS.gene042317:CDS
AATGTGTTGATAATGGTGAC+TGG 0.575398 2.4:+36293299 None:intergenic
CTCTTAACCTTCCAGAGAGA+GGG 0.576626 2.4:+36293078 None:intergenic
ATGGTGACTGGAATATTCAA+AGG 0.586441 2.4:+36293311 None:intergenic
CTTTGTCATTGTATCTGTGA+TGG 0.588884 2.4:-36292813 MS.gene042317:CDS
GATTGATAAAAGTAAACTGT+CGG 0.588937 2.4:+36293182 None:intergenic
GTTGAGCAAGTGATTTCAAG+TGG 0.594391 2.4:+36292556 None:intergenic
TCTCTTAACCTTCCAGAGAG+AGG 0.594993 2.4:+36293077 None:intergenic
AAAATCTCTCTGTTCCAACA+AGG 0.600364 2.4:+36292717 None:intergenic
GAGTTCATGAGAATTGATGA+AGG 0.601902 2.4:-36292654 MS.gene042317:CDS
GAAAGATATAAAGAGTCACC+TGG 0.606953 2.4:+36293242 None:intergenic
TTCGGAGAAGAAGAAGAACA+AGG 0.616846 2.4:-36293026 MS.gene042317:CDS
AATAGGCTTTGATGGAGTAA+CGG 0.618575 2.4:+36293274 None:intergenic
GTTACCTGAGCTATAACAAG+TGG 0.625798 2.4:+36292873 None:intergenic
GGAGAAGGTGATTCTAGTGG+TGG 0.632681 2.4:-36292060 MS.gene042317:CDS
AACATAGCTTTATCTGAACT+AGG 0.638913 2.4:-36292978 MS.gene042317:CDS
CAACATTGAATCAATAACCA+AGG 0.646066 2.4:+36291981 None:intergenic
GAATATTCAAAGGATTGAGA+GGG 0.647428 2.4:+36293321 None:intergenic
GAAGGAGAAGGTGATTCTAG+TGG 0.654729 2.4:-36292063 MS.gene042317:CDS
CATCACAGATACAATGACAA+AGG 0.659886 2.4:+36292814 None:intergenic
AGCTCGACAAAGTGTAACCG+AGG 0.683483 2.4:-36292277 MS.gene042317:CDS
CCAAAACATCAGCTAGAGCG+CGG 0.685011 2.4:+36293111 None:intergenic
AATGACAAAGGCGTAAACCG+AGG 0.712450 2.4:+36292826 None:intergenic
GGGAGTTCTTGAACCCATGG+AGG 0.731086 2.4:+36293341 None:intergenic
GCGCTCCAATCACATCATCG+AGG 0.780384 2.4:+36293216 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATAATTGTTCTGATTT+TGG - chr2.4:36293163-36293182 MS.gene042317:CDS 15.0%
!! TTTGGAAATTTGAATATCAA+TGG + chr2.4:36292398-36292417 None:intergenic 20.0%
! ACTTTGTATTGTTCTTCATT+TGG + chr2.4:36292416-36292435 None:intergenic 25.0%
! CAAACTTAACAATGAAACTT+TGG - chr2.4:36292690-36292709 MS.gene042317:CDS 25.0%
! GATTGATAAAAGTAAACTGT+CGG + chr2.4:36292140-36292159 None:intergenic 25.0%
! GTAAACTTACAATTACTCAA+TGG - chr2.4:36293128-36293147 MS.gene042317:CDS 25.0%
!! ATAATTGTTCTGATTTTGGA+TGG - chr2.4:36293167-36293186 MS.gene042317:CDS 25.0%
!! TAATTGTTCTGATTTTGGAT+GGG - chr2.4:36293168-36293187 MS.gene042317:CDS 25.0%
!! TTGATTATGTTGAAGTTGAT+AGG - chr2.4:36293083-36293102 MS.gene042317:CDS 25.0%
AAAAATCCACCATTGAAAGA+AGG - chr2.4:36292923-36292942 MS.gene042317:CDS 30.0%
AAAATCCACCATTGAAAGAA+GGG - chr2.4:36292924-36292943 MS.gene042317:CDS 30.0%
AACAGCAAAAACAGGAAAAT+TGG - chr2.4:36292565-36292584 MS.gene042317:CDS 30.0%
AACCTTAATTCGTAATCTTG+CGG + chr2.4:36292872-36292891 None:intergenic 30.0%
AAGTACTTCTTTCCAAAAAC+CGG - chr2.4:36292169-36292188 MS.gene042317:CDS 30.0%
AGAAGTGAAATTTCCACATA+TGG - chr2.4:36292643-36292662 MS.gene042317:CDS 30.0%
AGTTGATCTCAAATATTCCT+CGG + chr2.4:36293062-36293081 None:intergenic 30.0%
ATATGTGGAAATTTCACTTC+TGG + chr2.4:36292644-36292663 None:intergenic 30.0%
CAACATTGAATCAATAACCA+AGG + chr2.4:36293341-36293360 None:intergenic 30.0%
GAATATTCAAAGGATTGAGA+GGG + chr2.4:36292001-36292020 None:intergenic 30.0%
GGTAAAAATACACAAACTTG+AGG + chr2.4:36293235-36293254 None:intergenic 30.0%
TGAAGAACAATACAAAGTGT+TGG - chr2.4:36292418-36292437 MS.gene042317:CDS 30.0%
! AATTGTTCTGATTTTGGATG+GGG - chr2.4:36293169-36293188 MS.gene042317:CDS 30.0%
!! AACATAGCTTTATCTGAACT+AGG - chr2.4:36292341-36292360 MS.gene042317:CDS 30.0%
!! GTTTGTGTATTTTTACCTGA+AGG - chr2.4:36293238-36293257 MS.gene042317:CDS 30.0%
!!! GGTAAACTGGAAGTATTTTT+CGG - chr2.4:36292275-36292294 MS.gene042317:CDS 30.0%
AAAATCTCTCTGTTCCAACA+AGG + chr2.4:36292605-36292624 None:intergenic 35.0%
AAGGTTTCTGATCTTGTGAA+TGG - chr2.4:36292986-36293005 MS.gene042317:CDS 35.0%
AATACACAAACTTGAGGTGT+AGG + chr2.4:36293229-36293248 None:intergenic 35.0%
AATATCAATGGTTCCCAAGA+TGG + chr2.4:36292386-36292405 None:intergenic 35.0%
AGGTTAAGAGAGACGAAAAA+CGG - chr2.4:36292254-36292273 MS.gene042317:CDS 35.0%
ATGGTGACTGGAATATTCAA+AGG + chr2.4:36292011-36292030 None:intergenic 35.0%
ATTCTCATGAACTCCATATG+TGG + chr2.4:36292659-36292678 None:intergenic 35.0%
CATCACAGATACAATGACAA+AGG + chr2.4:36292508-36292527 None:intergenic 35.0%
CCATAAAACCCTTCTTTCAA+TGG + chr2.4:36292935-36292954 None:intergenic 35.0%
CTTTGTCATTGTATCTGTGA+TGG - chr2.4:36292506-36292525 MS.gene042317:CDS 35.0%
GAAAGATATAAAGAGTCACC+TGG + chr2.4:36292080-36292099 None:intergenic 35.0%
GAGTTCATGAGAATTGATGA+AGG - chr2.4:36292665-36292684 MS.gene042317:CDS 35.0%
GGAATATTCAAAGGATTGAG+AGG + chr2.4:36292002-36292021 None:intergenic 35.0%
GGTGTAGGTGTTAAATCAAT+AGG + chr2.4:36293214-36293233 None:intergenic 35.0%
TAAAACCCTTCTTTCAATGG+TGG + chr2.4:36292932-36292951 None:intergenic 35.0%
TATTGCTTGCACAACAAGTA+AGG - chr2.4:36292967-36292986 MS.gene042317:CDS 35.0%
TCAATAGGACCAGCATAAAT+TGG + chr2.4:36293199-36293218 None:intergenic 35.0%
TGCACATATTTGGAGATCAT+GGG - chr2.4:36292826-36292845 MS.gene042317:CDS 35.0%
TTGCACTACATTTGATGCAA+TGG - chr2.4:36292802-36292821 MS.gene042317:CDS 35.0%
! AACGGAGAATGTGTTGATAA+TGG + chr2.4:36292030-36292049 None:intergenic 35.0%
! AATAGGCTTTGATGGAGTAA+CGG + chr2.4:36292048-36292067 None:intergenic 35.0%
! ATTACCAGAGACTACTCTTT+TGG - chr2.4:36293012-36293031 MS.gene042317:CDS 35.0%
! CAGGTAACAATTTTCACATG+TGG - chr2.4:36292461-36292480 MS.gene042317:CDS 35.0%
! CATTGTATCTGTGATGGAAT+GGG - chr2.4:36292512-36292531 MS.gene042317:CDS 35.0%
! CCATTGAAAGAAGGGTTTTA+TGG - chr2.4:36292932-36292951 MS.gene042317:CDS 35.0%
! GTAACAATTTTCACATGTGG+TGG - chr2.4:36292464-36292483 MS.gene042317:CDS 35.0%
! TCATTGTATCTGTGATGGAA+TGG - chr2.4:36292511-36292530 MS.gene042317:CDS 35.0%
!! AATGTGTTGATAATGGTGAC+TGG + chr2.4:36292023-36292042 None:intergenic 35.0%
!! AGAAGGGTTTTATGGTAATG+TGG - chr2.4:36292940-36292959 MS.gene042317:CDS 35.0%
!! GTATTTTTACCTGAAGGAGA+AGG - chr2.4:36293244-36293263 MS.gene042317:CDS 35.0%
AAACCGGTTGCAGAAATACT+TGG - chr2.4:36292185-36292204 MS.gene042317:CDS 40.0%
AGAGACGAAAAACGGTAAAC+TGG - chr2.4:36292262-36292281 MS.gene042317:CDS 40.0%
AGCCGCAAGATTACGAATTA+AGG - chr2.4:36292867-36292886 MS.gene042317:CDS 40.0%
ATGCAATGGCTGCACATATT+TGG - chr2.4:36292816-36292835 MS.gene042317:CDS 40.0%
ATGCGATAACACTTCTGAAC+AGG + chr2.4:36292725-36292744 None:intergenic 40.0%
CTGCACATATTTGGAGATCA+TGG - chr2.4:36292825-36292844 MS.gene042317:CDS 40.0%
GAACAAGTTTACACAAGCCT+TGG - chr2.4:36293321-36293340 MS.gene042317:CDS 40.0%
GTTACCTGAGCTATAACAAG+TGG + chr2.4:36292449-36292468 None:intergenic 40.0%
GTTGAGCAAGTGATTTCAAG+TGG + chr2.4:36292766-36292785 None:intergenic 40.0%
TCAAACCTCGATGATGTGAT+TGG - chr2.4:36292098-36292117 MS.gene042317:CDS 40.0%
TGTGGAAATTTCACTTCTGG+TGG + chr2.4:36292641-36292660 None:intergenic 40.0%
TTACAGAACCTGAACCTTGT+TGG - chr2.4:36292588-36292607 MS.gene042317:CDS 40.0%
TTCGGAGAAGAAGAAGAACA+AGG - chr2.4:36292293-36292312 MS.gene042317:CDS 40.0%
! AATGGTTCCCAAGATGGATT+TGG + chr2.4:36292380-36292399 None:intergenic 40.0%
! TATGCAGTTTCTAGGAGCAT+GGG - chr2.4:36292538-36292557 MS.gene042317:CDS 40.0%
! TTGACAGGAATAGGCTTTGA+TGG + chr2.4:36292056-36292075 None:intergenic 40.0%
!! AATGCCACTTGTTATAGCTC+AGG - chr2.4:36292442-36292461 MS.gene042317:CDS 40.0%
!! AGAAGAACAAGGAGCGTTAA+TGG - chr2.4:36292304-36292323 MS.gene042317:CDS 40.0%
!! GTATTTCTGCAACCGGTTTT+TGG + chr2.4:36292184-36292203 None:intergenic 40.0%
AAGCCTATTCCTGTCAAACC+AGG - chr2.4:36292059-36292078 MS.gene042317:CDS 45.0%
AGGCCAAAGCAACTTGAGTT+TGG - chr2.4:36293103-36293122 MS.gene042317:CDS 45.0%
ATGGGAGCTATGCAGTTTCT+AGG - chr2.4:36292530-36292549 MS.gene042317:CDS 45.0%
CCAAAGCAACTTGAGTTTGG+TGG - chr2.4:36293106-36293125 MS.gene042317:CDS 45.0%
CGAACCAAAAGAGTAGTCTC+TGG + chr2.4:36293019-36293038 None:intergenic 45.0%
CTAGAATCACCTTCTCCTTC+AGG + chr2.4:36293256-36293275 None:intergenic 45.0%
CTCTTAACCTTCCAGAGAGA+GGG + chr2.4:36292244-36292263 None:intergenic 45.0%
CTTCCAGAGAGAGGGTAATA+GGG + chr2.4:36292236-36292255 None:intergenic 45.0%
GAAGGAGAAGGTGATTCTAG+TGG - chr2.4:36293256-36293275 MS.gene042317:CDS 45.0%
GCTCAACCATTTGATTCTGC+TGG - chr2.4:36292779-36292798 MS.gene042317:CDS 45.0%
GTGCAACCAGCAGAATCAAA+TGG + chr2.4:36292788-36292807 None:intergenic 45.0%
TCTCTGTTCCAACAAGGTTC+AGG + chr2.4:36292599-36292618 None:intergenic 45.0%
TCTCTTAACCTTCCAGAGAG+AGG + chr2.4:36292245-36292264 None:intergenic 45.0%
TGCTGCTCCAAATCCATCTT+GGG - chr2.4:36292370-36292389 MS.gene042317:CDS 45.0%
TGGGGTAAGCCAATTTATGC+TGG - chr2.4:36293187-36293206 MS.gene042317:CDS 45.0%
TTGCTGCTCCAAATCCATCT+TGG - chr2.4:36292369-36292388 MS.gene042317:CDS 45.0%
! AAAGAGTCACCTGGTTTGAC+AGG + chr2.4:36292071-36292090 None:intergenic 45.0%
! AACTTGTTCGCAGCAGATTC+AGG + chr2.4:36293310-36293329 None:intergenic 45.0%
! AATGACAAAGGCGTAAACCG+AGG + chr2.4:36292496-36292515 None:intergenic 45.0%
! CCACCAAACTCAAGTTGCTT+TGG + chr2.4:36293109-36293128 None:intergenic 45.0%
! CTATGCAGTTTCTAGGAGCA+TGG - chr2.4:36292537-36292556 MS.gene042317:CDS 45.0%
! TCACCTGGTTTGACAGGAAT+AGG + chr2.4:36292065-36292084 None:intergenic 45.0%
!! ACATGTGGTGGATTTAGCCT+CGG - chr2.4:36292476-36292495 MS.gene042317:CDS 45.0%
AGAGGGAGTTCTTGAACCCA+TGG + chr2.4:36291984-36292003 None:intergenic 50.0%
AGCTCGACAAAGTGTAACCG+AGG - chr2.4:36293042-36293061 MS.gene042317:CDS 50.0%
CCAAAACATCAGCTAGAGCG+CGG + chr2.4:36292211-36292230 None:intergenic 50.0%
CCTATTACCCTCTCTCTGGA+AGG - chr2.4:36292234-36292253 MS.gene042317:CDS 50.0%
CCTTCCAGAGAGAGGGTAAT+AGG + chr2.4:36292237-36292256 None:intergenic 50.0%
GGAGAAGGTGATTCTAGTGG+TGG - chr2.4:36293259-36293278 MS.gene042317:CDS 50.0%
GTTCCCTATTACCCTCTCTC+TGG - chr2.4:36292230-36292249 MS.gene042317:CDS 50.0%
TGGGCTGCAACAGCAAAAAC+AGG - chr2.4:36292557-36292576 MS.gene042317:CDS 50.0%
! CGGCCAAGTATTTCTGCAAC+CGG + chr2.4:36292191-36292210 None:intergenic 50.0%
! TTCACTTCTGGTGGATCACG+TGG + chr2.4:36292632-36292651 None:intergenic 50.0%
GCGCTCCAATCACATCATCG+AGG + chr2.4:36292106-36292125 None:intergenic 55.0%
GGGAGTTCTTGAACCCATGG+AGG + chr2.4:36291981-36292000 None:intergenic 55.0%
! CCGCGCTCTAGCTGATGTTT+TGG - chr2.4:36292208-36292227 MS.gene042317:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 36291972 36293369 36291972 ID=MS.gene042317
chr2.4 mRNA 36291972 36293369 36291972 ID=MS.gene042317.t1;Parent=MS.gene042317
chr2.4 exon 36291972 36293369 36291972 ID=MS.gene042317.t1.exon1;Parent=MS.gene042317.t1
chr2.4 CDS 36291972 36293369 36291972 ID=cds.MS.gene042317.t1;Parent=MS.gene042317.t1
Gene Sequence

>MS.gene042317

ATGGCTCCTCCATGGGTTCAAGAACTCCCTCTCAATCCTTTGAATATTCCAGTCACCATTATCAACACATTCTCCGTTACTCCATCAAAGCCTATTCCTGTCAAACCAGGTGACTCTTTATATCTTTCAAACCTCGATGATGTGATTGGAGCGCGCGTCTTCACACCGACAGTTTACTTTTATCAATCAGACAATTCAAGTACTTCTTTCCAAAAACCGGTTGCAGAAATACTTGGCCGCGCTCTAGCTGATGTTTTGGTTCCCTATTACCCTCTCTCTGGAAGGTTAAGAGAGACGAAAAACGGTAAACTGGAAGTATTTTTCGGAGAAGAAGAAGAACAAGGAGCGTTAATGGTTGAAGCAAGATCAAACATAGCTTTATCTGAACTAGGTGATTTTGCTGCTCCAAATCCATCTTGGGAACCATTGATATTCAAATTTCCAAATGAAGAACAATACAAAGTGTTGGAAATGCCACTTGTTATAGCTCAGGTAACAATTTTCACATGTGGTGGATTTAGCCTCGGTTTACGCCTTTGTCATTGTATCTGTGATGGAATGGGAGCTATGCAGTTTCTAGGAGCATGGGCTGCAACAGCAAAAACAGGAAAATTGGTTACAGAACCTGAACCTTGTTGGAACAGAGAGATTTTCAAGCCACGTGATCCACCAGAAGTGAAATTTCCACATATGGAGTTCATGAGAATTGATGAAGGATCAAACTTAACAATGAAACTTTGGAAAACAAAGCCTGTTCAGAAGTGTTATCGCATAAGACGCGAGTTTCAAAACCACTTGAAATCACTTGCTCAACCATTTGATTCTGCTGGTTGCACTACATTTGATGCAATGGCTGCACATATTTGGAGATCATGGGTTAAAGCTCTTGATGTTAAGCCGCAAGATTACGAATTAAGGTTAACATTTTCAGTTAATGCTAGACAGAAACTTAAAAATCCACCATTGAAAGAAGGGTTTTATGGTAATGTGGTTTGTATTGCTTGCACAACAAGTAAGGTTTCTGATCTTGTGAATGGAAAATTACCAGAGACTACTCTTTTGGTTCGTGAAGCTCGACAAAGTGTAACCGAGGAATATTTGAGATCAACTGTTGATTATGTTGAAGTTGATAGGCCAAAGCAACTTGAGTTTGGTGGTAAACTTACAATTACTCAATGGACAAGATTTTCTATTTATAATTGTTCTGATTTTGGATGGGGTAAGCCAATTTATGCTGGTCCTATTGATTTAACACCTACACCTCAAGTTTGTGTATTTTTACCTGAAGGAGAAGGTGATTCTAGTGGTGGTTCTATGATTGTTTGCATTTGCTTGCCTGAATCTGCTGCGAACAAGTTTACACAAGCCTTGGTTATTGATTCAATGTTGTTGACATAG
Protein sequence

>MS.gene042317.t1

MAPPWVQELPLNPLNIPVTIINTFSVTPSKPIPVKPGDSLYLSNLDDVIGARVFTPTVYFYQSDNSSTSFQKPVAEILGRALADVLVPYYPLSGRLRETKNGKLEVFFGEEEEQGALMVEARSNIALSELGDFAAPNPSWEPLIFKFPNEEQYKVLEMPLVIAQVTIFTCGGFSLGLRLCHCICDGMGAMQFLGAWAATAKTGKLVTEPEPCWNREIFKPRDPPEVKFPHMEFMRIDEGSNLTMKLWKTKPVQKCYRIRREFQNHLKSLAQPFDSAGCTTFDAMAAHIWRSWVKALDVKPQDYELRLTFSVNARQKLKNPPLKEGFYGNVVCIACTTSKVSDLVNGKLPETTLLVREARQSVTEEYLRSTVDYVEVDRPKQLEFGGKLTITQWTRFSIYNCSDFGWGKPIYAGPIDLTPTPQVCVFLPEGEGDSSGGSMIVCICLPESAANKFTQALVIDSMLLT