AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86782

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86782.t1 MTR_3g030540 93.135 437 30 0 4 440 1 437 0.0 831
MS.gene86782.t1 MTR_2g060350 62.247 445 158 6 4 440 1 443 0.0 558
MS.gene86782.t1 MTR_1g052315 50.115 433 202 3 16 438 2 430 9.84e-145 421
MS.gene86782.t1 MTR_7g091850 50.457 438 208 4 9 438 7 443 3.25e-140 409
MS.gene86782.t1 MTR_7g091840 48.942 378 183 5 9 382 7 378 1.47e-118 351
MS.gene86782.t1 MTR_2g103410 46.594 367 193 2 73 436 59 425 2.91e-110 332
MS.gene86782.t1 MTR_8g065030 44.565 368 202 2 49 414 41 408 8.73e-109 329
MS.gene86782.t1 MTR_6g005630 44.886 352 185 6 69 418 41 385 7.74e-97 297
MS.gene86782.t1 MTR_2g049400 41.476 393 217 9 48 437 6 388 5.03e-87 271
MS.gene86782.t1 MTR_4g084050 40.104 384 204 8 63 431 13 385 3.90e-85 266
MS.gene86782.t1 MTR_7g110050 40.111 359 195 6 74 417 68 421 3.61e-84 266
MS.gene86782.t1 MTR_3g084360 39.516 372 210 6 67 431 18 381 8.06e-83 260
MS.gene86782.t1 MTR_1g086390 40.214 373 206 8 73 431 81 450 8.35e-83 263
MS.gene86782.t1 MTR_3g084380 40.489 368 200 7 54 412 2 359 1.25e-82 259
MS.gene86782.t1 MTR_3g438050 42.363 347 188 8 71 411 25 365 2.24e-82 259
MS.gene86782.t1 MTR_6g005630 42.045 352 172 8 69 418 41 362 2.41e-82 259
MS.gene86782.t1 MTR_8g065010 38.320 381 199 7 65 411 94 472 1.12e-81 261
MS.gene86782.t1 MTR_3g054320 40.833 360 192 8 74 425 31 377 1.18e-81 257
MS.gene86782.t1 MTR_4g094715 41.360 353 186 7 74 411 66 412 1.48e-81 259
MS.gene86782.t1 MTR_4g083660 42.175 377 206 10 66 437 2 371 1.89e-81 256
MS.gene86782.t1 MTR_6g028080 40.108 369 195 8 72 427 29 384 2.67e-80 254
MS.gene86782.t1 MTR_5g033680 37.709 358 214 5 61 411 47 402 9.93e-80 254
MS.gene86782.t1 MTR_8g009810 41.600 375 208 9 70 439 32 400 3.62e-79 251
MS.gene86782.t1 MTR_5g034090 37.306 386 206 9 59 411 103 485 1.96e-78 253
MS.gene86782.t1 MTR_5g043350 39.130 368 210 5 66 426 22 382 2.50e-78 249
MS.gene86782.t1 MTR_7g113910 48.062 258 116 1 166 423 95 334 8.42e-78 246
MS.gene86782.t1 MTR_2g435640 38.172 372 215 11 70 432 50 415 1.76e-77 247
MS.gene86782.t1 MTR_2g103650 40.580 345 197 5 72 409 27 370 3.25e-77 246
MS.gene86782.t1 MTR_8g010080 39.888 356 194 7 74 425 32 371 1.20e-76 244
MS.gene86782.t1 MTR_1g088160 39.601 351 195 11 74 415 36 378 2.21e-76 244
MS.gene86782.t1 MTR_8g030930 40.811 370 198 10 55 415 15 372 4.15e-76 243
MS.gene86782.t1 MTR_4g120320 39.370 381 214 12 70 440 49 422 1.11e-73 237
MS.gene86782.t1 MTR_1g012390 38.798 366 207 9 69 426 24 380 3.94e-73 235
MS.gene86782.t1 MTR_2g103410 46.718 259 135 2 73 328 59 317 8.89e-72 229
MS.gene86782.t1 MTR_8g010090 38.375 357 199 8 74 425 32 372 1.06e-70 228
MS.gene86782.t1 MTR_2g032710 38.292 363 211 6 60 411 54 414 3.22e-70 229
MS.gene86782.t1 MTR_2g058670 37.569 362 212 8 73 426 25 380 3.31e-70 228
MS.gene86782.t1 MTR_8g010110 38.375 357 199 8 74 425 32 372 4.26e-70 227
MS.gene86782.t1 MTR_2g058840 37.604 359 214 7 73 426 25 378 4.42e-70 228
MS.gene86782.t1 MTR_8g010040 38.874 373 206 10 70 437 29 384 7.70e-70 226
MS.gene86782.t1 MTR_0157s0060 40.169 356 194 5 77 425 2 345 1.93e-69 224
MS.gene86782.t1 MTR_4g034020 40.169 356 194 5 77 425 2 345 1.93e-69 224
MS.gene86782.t1 MTR_1g013740 36.631 374 210 9 70 437 32 384 6.44e-69 224
MS.gene86782.t1 MTR_8g010070 38.719 359 196 12 74 425 32 373 1.89e-67 220
MS.gene86782.t1 MTR_2g058640 37.709 358 206 8 91 438 41 391 1.28e-66 218
MS.gene86782.t1 MTR_2g029170 38.485 330 194 8 111 437 35 358 2.90e-66 216
MS.gene86782.t1 MTR_8g008765 38.602 329 183 6 74 398 32 345 4.46e-66 215
MS.gene86782.t1 MTR_1g013730 35.657 373 213 9 70 436 24 375 1.53e-65 215
MS.gene86782.t1 MTR_7g035415 41.017 295 154 7 71 357 38 320 6.03e-65 212
MS.gene86782.t1 MTR_5g063430 37.459 307 178 6 63 364 15 312 2.82e-59 196
MS.gene86782.t1 MTR_5g034090 36.634 303 158 7 59 328 103 404 1.61e-57 195
MS.gene86782.t1 MTR_8g077320 39.735 302 137 7 71 357 31 302 2.68e-57 193
MS.gene86782.t1 MTR_8g008700 41.154 260 136 4 150 405 31 277 1.20e-56 189
MS.gene86782.t1 MTR_5g097860 34.056 323 202 7 71 387 23 340 1.80e-55 187
MS.gene86782.t1 MTR_8g010050 33.520 358 182 11 74 425 32 339 6.18e-50 173
MS.gene86782.t1 MTR_8g031180 44.231 208 108 2 211 413 70 274 2.74e-48 167
MS.gene86782.t1 MTR_2g100040 31.356 354 185 11 63 409 14 316 1.10e-41 151
MS.gene86782.t1 MTR_2g042740 28.528 326 198 11 138 437 128 444 5.51e-26 110
MS.gene86782.t1 MTR_4g124980 25.412 425 257 18 54 436 55 461 1.57e-23 103
MS.gene86782.t1 MTR_3g449590 25.000 396 257 12 63 426 37 424 3.51e-22 99.0
MS.gene86782.t1 MTR_1g086030 29.825 285 167 7 125 382 37 315 1.69e-21 96.3
MS.gene86782.t1 MTR_7g110710 29.573 328 191 14 140 440 117 431 7.77e-21 95.1
MS.gene86782.t1 MTR_7g110710 29.573 328 191 14 140 440 117 431 1.21e-20 94.4
MS.gene86782.t1 MTR_4g120730 27.891 294 187 9 159 436 143 427 2.16e-20 93.6
MS.gene86782.t1 MTR_1g086030 31.481 270 152 8 140 382 156 419 1.03e-19 91.7
MS.gene86782.t1 MTR_7g078680 24.923 325 215 8 137 438 126 444 2.90e-19 90.5
MS.gene86782.t1 MTR_5g090160 25.130 386 238 12 63 411 34 405 2.95e-19 90.1
MS.gene86782.t1 MTR_3g096330 26.756 299 186 10 159 438 179 463 1.40e-18 89.0
MS.gene86782.t1 MTR_8g098840 25.279 269 177 8 158 411 146 405 1.78e-18 87.8
MS.gene86782.t1 MTR_8g028725 24.874 398 263 13 71 438 53 444 3.41e-18 87.0
MS.gene86782.t1 MTR_7g088550 28.309 272 166 10 158 411 152 412 7.63e-18 85.9
MS.gene86782.t1 MTR_1g100060 25.589 297 187 10 140 411 167 454 1.01e-17 85.9
MS.gene86782.t1 MTR_1g100050 26.022 269 175 8 158 411 152 411 2.34e-17 84.3
MS.gene86782.t1 MTR_2g435340 25.538 325 208 9 130 436 51 359 1.90e-16 81.3
MS.gene86782.t1 MTR_2g435340 25.397 315 202 8 130 427 116 414 2.93e-16 80.9
MS.gene86782.t1 MTR_1g099850 24.762 315 200 12 140 427 129 433 8.36e-16 79.7
MS.gene86782.t1 MTR_1g100040 25.436 287 183 9 140 404 133 410 2.27e-15 78.6
MS.gene86782.t1 MTR_8g007320 50.847 59 28 1 367 425 22 79 3.07e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86782.t1 AT3G07970 53.675 449 183 10 4 438 1 438 9.25e-156 448
MS.gene86782.t1 AT3G57510 46.759 432 212 7 13 438 11 430 2.59e-129 380
MS.gene86782.t1 AT2G41850 47.465 434 213 7 9 438 8 430 1.97e-120 358
MS.gene86782.t1 AT1G80170 46.015 389 204 4 53 436 33 420 3.73e-116 347
MS.gene86782.t1 AT1G70500 42.694 438 230 9 9 436 5 431 2.31e-107 326
MS.gene86782.t1 AT1G23460 44.855 379 204 4 61 436 31 407 2.79e-107 324
MS.gene86782.t1 AT1G23460 44.855 379 204 4 61 436 55 431 4.12e-107 325
MS.gene86782.t1 AT1G56710 42.553 376 184 9 61 411 29 397 6.18e-91 282
MS.gene86782.t1 AT5G14650 41.047 363 206 7 51 411 30 386 2.24e-88 276
MS.gene86782.t1 AT4G35670 42.582 364 194 7 74 431 25 379 8.49e-87 270
MS.gene86782.t1 AT2G43890 38.000 400 232 10 41 437 3 389 1.17e-85 267
MS.gene86782.t1 AT5G27530 42.618 359 183 6 74 426 54 395 5.42e-85 268
MS.gene86782.t1 AT5G44840 40.111 359 201 6 74 426 25 375 5.81e-84 263
MS.gene86782.t1 AT1G05660 39.683 378 217 9 63 436 18 388 1.26e-83 262
MS.gene86782.t1 AT3G15720 42.120 368 191 9 69 426 20 375 1.57e-83 264
MS.gene86782.t1 AT3G15720 42.120 368 191 9 69 426 19 374 1.57e-83 264
MS.gene86782.t1 AT4G18180 39.062 384 210 8 73 436 39 418 1.67e-82 260
MS.gene86782.t1 AT2G43870 41.755 376 204 13 69 438 17 383 1.79e-82 259
MS.gene86782.t1 AT1G05650 39.617 366 212 8 74 436 29 388 3.65e-82 258
MS.gene86782.t1 AT2G43860 42.785 395 206 13 51 439 18 398 1.10e-81 258
MS.gene86782.t1 AT1G02460 41.783 359 181 9 74 411 90 441 1.64e-81 260
MS.gene86782.t1 AT3G15720 41.689 367 191 9 69 426 19 371 3.37e-81 258
MS.gene86782.t1 AT3G59850 41.779 371 202 12 72 436 66 428 7.00e-81 256
MS.gene86782.t1 AT2G43880 40.000 375 215 9 66 437 24 391 8.58e-81 255
MS.gene86782.t1 AT3G59850 41.779 371 202 12 72 436 22 384 9.63e-81 254
MS.gene86782.t1 AT1G80170 46.071 280 146 3 53 328 33 311 2.10e-80 251
MS.gene86782.t1 AT3G26610 35.748 428 246 10 1 415 1 412 4.45e-79 253
MS.gene86782.t1 AT1G80140 40.708 339 186 7 108 439 6 336 7.46e-79 248
MS.gene86782.t1 AT1G48100 37.640 356 201 7 74 414 81 430 2.60e-77 248
MS.gene86782.t1 AT3G15720 43.750 320 162 7 114 426 3 311 8.13e-77 244
MS.gene86782.t1 AT1G60590 36.314 369 202 6 74 411 124 490 1.26e-76 248
MS.gene86782.t1 AT1G10640 38.798 366 194 6 74 411 120 483 1.36e-76 248
MS.gene86782.t1 AT4G01890 37.666 377 209 8 61 417 53 423 3.31e-76 245
MS.gene86782.t1 AT1G65570 37.968 374 221 9 68 436 26 393 4.02e-76 243
MS.gene86782.t1 AT4G32380 39.255 349 197 7 73 413 26 367 1.21e-75 243
MS.gene86782.t1 AT1G02790 34.325 437 237 12 17 438 19 420 1.69e-75 242
MS.gene86782.t1 AT3G07830 37.845 362 213 8 82 437 30 385 1.03e-74 239
MS.gene86782.t1 AT4G13760 38.035 397 224 11 52 437 15 400 1.06e-74 239
MS.gene86782.t1 AT2G33160 38.060 402 231 10 44 436 7 399 1.27e-74 246
MS.gene86782.t1 AT5G48140 37.097 372 221 8 72 437 20 384 1.72e-74 239
MS.gene86782.t1 AT5G44830 39.692 325 177 4 104 426 9 316 2.97e-74 236
MS.gene86782.t1 AT4G35670 47.510 261 129 2 152 412 1 253 4.82e-74 234
MS.gene86782.t1 AT5G17200 38.482 369 193 7 69 413 35 393 1.43e-73 237
MS.gene86782.t1 AT2G40310 37.531 397 226 11 52 437 15 400 9.01e-73 234
MS.gene86782.t1 AT3G07820 36.828 372 223 8 72 437 19 384 3.99e-72 232
MS.gene86782.t1 AT1G17150 38.211 369 217 8 74 436 34 397 1.57e-71 231
MS.gene86782.t1 AT1G43080 36.476 403 234 11 46 437 9 400 2.26e-71 231
MS.gene86782.t1 AT3G07840 36.193 373 224 8 72 437 20 385 3.10e-70 228
MS.gene86782.t1 AT1G43090 36.432 398 231 11 46 432 9 395 4.47e-70 229
MS.gene86782.t1 AT2G15460 36.203 395 230 11 54 437 17 400 2.32e-69 226
MS.gene86782.t1 AT1G43100 36.432 398 231 11 46 432 9 395 7.79e-69 225
MS.gene86782.t1 AT2G15450 36.020 397 231 12 54 438 17 402 1.63e-68 223
MS.gene86782.t1 AT1G78400 36.639 363 219 8 70 426 32 389 7.31e-68 222
MS.gene86782.t1 AT3G07850 39.402 368 215 7 73 436 70 433 8.58e-68 223
MS.gene86782.t1 AT3G14040 39.024 369 217 7 72 436 70 434 1.68e-67 222
MS.gene86782.t1 AT2G15470 35.696 395 232 11 54 437 17 400 9.70e-67 219
MS.gene86782.t1 AT2G26620 36.193 373 223 9 74 437 32 398 3.96e-65 214
MS.gene86782.t1 AT4G32375 40.794 277 155 3 149 423 9 278 2.97e-63 210
MS.gene86782.t1 AT4G32370 38.312 308 170 8 68 363 29 328 1.48e-62 206
MS.gene86782.t1 AT5G14650 39.286 280 164 5 51 328 30 305 2.41e-62 204
MS.gene86782.t1 AT2G43880 40.000 305 174 8 66 367 24 322 3.27e-61 202
MS.gene86782.t1 AT4G32380 35.447 347 172 7 73 413 26 326 5.29e-60 201
MS.gene86782.t1 AT5G39910 31.186 388 189 6 71 437 34 364 5.75e-53 182
MS.gene86782.t1 AT3G42950 27.960 397 242 13 74 436 67 453 9.60e-27 112
MS.gene86782.t1 AT3G62110 26.570 414 249 15 48 426 27 420 3.64e-26 110
MS.gene86782.t1 AT3G62110 26.570 414 249 15 48 426 27 420 3.64e-26 110
MS.gene86782.t1 AT4G23820 26.884 398 247 12 63 427 31 417 2.52e-24 105
MS.gene86782.t1 AT1G19170 24.464 466 291 16 12 437 32 476 9.68e-24 103
MS.gene86782.t1 AT2G23900 26.568 271 172 8 158 411 158 418 4.85e-19 89.7
MS.gene86782.t1 AT3G48950 25.503 298 187 9 140 411 119 407 8.25e-19 89.0
MS.gene86782.t1 AT4G33440 27.036 307 185 10 154 438 170 459 2.13e-18 87.8
MS.gene86782.t1 AT5G41870 24.806 387 242 13 63 413 35 408 4.80e-18 86.7
MS.gene86782.t1 AT5G49215 27.881 269 170 6 161 414 148 407 1.52e-17 85.1
MS.gene86782.t1 AT5G49215 27.881 269 170 6 161 414 146 405 1.67e-17 84.7
MS.gene86782.t1 AT3G16850 25.503 298 192 8 147 427 131 415 1.21e-16 82.4
MS.gene86782.t1 AT3G06770 26.837 313 196 8 147 440 47 345 1.42e-16 81.3
MS.gene86782.t1 AT4G23500 26.050 238 155 6 158 381 170 400 2.52e-16 81.6
MS.gene86782.t1 AT3G06770 26.837 313 196 8 147 440 65 363 2.77e-16 80.5
MS.gene86782.t1 AT3G06770 26.837 313 196 8 147 440 65 363 2.77e-16 80.5
MS.gene86782.t1 AT3G06770 26.837 313 196 8 147 440 134 432 5.35e-16 80.1
MS.gene86782.t1 AT3G06770 26.837 313 196 8 147 440 135 433 5.67e-16 80.1
MS.gene86782.t1 AT3G61490 24.062 320 220 7 131 433 131 444 6.42e-16 80.1
MS.gene86782.t1 AT3G61490 24.062 320 220 7 131 433 229 542 7.89e-16 80.1
MS.gene86782.t1 AT3G61490 24.062 320 220 7 131 433 116 429 1.23e-15 79.3
MS.gene86782.t1 AT3G61490 24.062 320 220 7 131 433 116 429 1.23e-15 79.3
MS.gene86782.t1 AT3G61490 24.062 320 220 7 131 433 116 429 1.23e-15 79.3

Find 89 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACCCAATACTGCATTAA+AGG 0.186184 3.4:+23857137 MS.gene86782:CDS
GCCTCATTTGGTTTGTGTTT+TGG 0.200442 3.4:+23856499 MS.gene86782:CDS
CAAACCAAATGAGGCTAAAA+TGG 0.240282 3.4:-23856491 None:intergenic
GTTTCATTATGTTAGATCTA+TGG 0.262928 3.4:+23857244 MS.gene86782:intron
ATCTAAATGCAGGGAGGTTC+TGG 0.263116 3.4:+23859842 MS.gene86782:intron
CATGGCCCTGAAAATGTTAT+AGG 0.267346 3.4:-23857114 None:intergenic
GCTCCATATGCTGAAATATT+AGG 0.274821 3.4:-23857283 None:intergenic
TTGAAGGATGCAACAATGTT+TGG 0.280649 3.4:+23858128 MS.gene86782:CDS
ACATGAACAATTTAGTTTCA+TGG 0.283475 3.4:+23856591 MS.gene86782:CDS
TTTACCTTTAATGCAGTATT+GGG 0.302256 3.4:-23857141 None:intergenic
GTGATGTACCAAAACATAAA+AGG 0.305023 3.4:+23860466 MS.gene86782:CDS
AACTTGTCCATCATTAGATT+TGG 0.311900 3.4:-23856686 None:intergenic
ATGCACAACAAATGCATATA+AGG 0.313150 3.4:+23858104 MS.gene86782:CDS
AAACTAAATTGTTCATGTAT+TGG 0.329621 3.4:-23856586 None:intergenic
GATGGTGGTGGTACCATTAA+TGG 0.331489 3.4:+23857355 MS.gene86782:CDS
AATATTAGGCCATGCTTTGA+TGG 0.334356 3.4:-23857269 None:intergenic
GTGAACAGAGCCACACTAAT+AGG 0.355997 3.4:+23859110 MS.gene86782:CDS
CTTTATTCTGACTCCATTAG+TGG 0.361879 3.4:-23859135 None:intergenic
ATTTACCTTTAATGCAGTAT+TGG 0.379250 3.4:-23857142 None:intergenic
ATAATTAACATTGTCACATT+TGG 0.385288 3.4:-23860602 None:intergenic
AGATGATAGACACTATGTTT+AGG 0.399830 3.4:-23857087 None:intergenic
TGCGGCGAAGGCGCGATAGA+TGG 0.401102 3.4:-23856628 None:intergenic
TGAAGGATGCAACAATGTTT+GGG 0.406265 3.4:+23858129 MS.gene86782:CDS
ACGGTTAACGTTGACGACTA+TGG 0.411063 3.4:+23856661 MS.gene86782:CDS
TATTGTAGCATTGGAAGCTT+AGG 0.412870 3.4:+23859050 MS.gene86782:intron
TGGCCTAATATTTCAGCATA+TGG 0.414557 3.4:+23857280 MS.gene86782:CDS
TTCACCAACCTCTCATTGTA+AGG 0.419899 3.4:-23856562 None:intergenic
AATGTTAATTATTCAAATAG+TGG 0.428526 3.4:+23860613 MS.gene86782:CDS
GTGGACCAGGACATGGAATC+AGG 0.439750 3.4:+23858917 MS.gene86782:CDS
TTATGAAAACATTCTCTGAA+TGG 0.443046 3.4:-23858213 None:intergenic
GGTGGTACCATTAATGGAAA+TGG 0.446333 3.4:+23857361 MS.gene86782:CDS
TATGAAAACATTCTCTGAAT+GGG 0.447211 3.4:-23858212 None:intergenic
TTTGTTCATGGGTAACACTT+AGG 0.447678 3.4:-23860646 None:intergenic
TTCATAACCAACAGCACCAT+TGG 0.449118 3.4:+23858229 MS.gene86782:CDS
ACACTAATAGGAACCACTAA+TGG 0.455342 3.4:+23859122 MS.gene86782:CDS
TGCAAGTGAATTCCTTTGCA+TGG 0.456208 3.4:-23860538 None:intergenic
CAAATGCATATAAGGATTGA+AGG 0.456482 3.4:+23858112 MS.gene86782:CDS
CCTTTAATGCAGTATTGGGT+TGG 0.462412 3.4:-23857137 None:intergenic
TTGGCTCTTACTTAGAAGAA+AGG 0.465871 3.4:+23856518 MS.gene86782:CDS
TGGAATGAGGCATGTTCTAA+AGG 0.468725 3.4:+23857048 MS.gene86782:CDS
TTTAGGCATTTGAAATGTCA+TGG 0.469564 3.4:+23857028 MS.gene86782:intron
ACCTGTCCCAATGGTGCTGT+TGG 0.469575 3.4:-23858236 None:intergenic
AACAATTTAGTTTCATGGCA+AGG 0.470742 3.4:+23856596 MS.gene86782:CDS
GTCACAGATCTCATAGTTGA+TGG 0.474884 3.4:+23857337 MS.gene86782:CDS
ATGGAGCAGATAGAAAGCTT+TGG 0.483983 3.4:+23857299 MS.gene86782:CDS
TCAGTGTTGGGACTGTGTCC+TGG 0.491323 3.4:-23858175 None:intergenic
GGAATGAGGCATGTTCTAAA+GGG 0.495884 3.4:+23857049 MS.gene86782:CDS
TCATAACCAACAGCACCATT+GGG 0.496240 3.4:+23858230 MS.gene86782:CDS
ATATGAATTCCATCAGTGTT+GGG 0.505639 3.4:-23858187 None:intergenic
GCAAGTGAATTCCTTTGCAT+GGG 0.507357 3.4:-23860537 None:intergenic
ACAATTTAGTTTCATGGCAA+GGG 0.511066 3.4:+23856597 MS.gene86782:CDS
AACATTGTCACATTTGGCAG+TGG 0.514203 3.4:-23860596 None:intergenic
AATATGAATTCCATCAGTGT+TGG 0.516408 3.4:-23858188 None:intergenic
ATTGTATTTCAATTGTAAGT+GGG 0.522080 3.4:+23858863 MS.gene86782:CDS
TGAATGCAATAATTTGAAAG+TGG 0.522545 3.4:+23858063 MS.gene86782:CDS
ATGAACCTGATTCCATGTCC+TGG 0.522587 3.4:-23858922 None:intergenic
AATGCAGTATTGGGTTGGCA+TGG 0.531497 3.4:-23857132 None:intergenic
TGTAGCATTGGAAGCTTAGG+AGG 0.531742 3.4:+23859053 MS.gene86782:intron
GATTGTATTTCAATTGTAAG+TGG 0.534722 3.4:+23858862 MS.gene86782:CDS
ATTTGAAATGTCATGGAATG+AGG 0.544227 3.4:+23857035 MS.gene86782:CDS
ACCAACAGCACCATTGGGAC+AGG 0.545836 3.4:+23858235 MS.gene86782:CDS
TTTATTTCACAGCCATGCAA+GGG 0.548985 3.4:+23857915 MS.gene86782:intron
TATGGAGCCAAATCTAATGA+TGG 0.549828 3.4:+23856679 MS.gene86782:CDS
TTCTAAAGGGAAGGTTCTTG+TGG 0.553586 3.4:+23857062 MS.gene86782:CDS
ATTAGTGGTTCCTATTAGTG+TGG 0.555263 3.4:-23859120 None:intergenic
TGATGGACAAGTTGATAATG+AGG 0.564129 3.4:+23856696 MS.gene86782:CDS
AGTCAGAATAAAGACTTGGC+AGG 0.564393 3.4:+23859145 MS.gene86782:CDS
GCCAAAACACAAACCAAATG+AGG 0.566883 3.4:-23856500 None:intergenic
TCAACCTTACAATGAGAGGT+TGG 0.581966 3.4:+23856558 MS.gene86782:CDS
AATCTAATAGTCACAGCACC+AGG 0.584074 3.4:+23858157 MS.gene86782:CDS
GGACACAGTCCCAACACTGA+TGG 0.584378 3.4:+23858178 MS.gene86782:CDS
ATTACTTGTGGACCAGGACA+TGG 0.585454 3.4:+23858910 MS.gene86782:CDS
ATCATCAACCTTACAATGAG+AGG 0.592562 3.4:+23856554 MS.gene86782:CDS
CGAGCAACTGATATTACTTG+TGG 0.594503 3.4:+23858898 MS.gene86782:CDS
AGCAAAGCTGTCCCATGCAA+AGG 0.594681 3.4:+23860526 MS.gene86782:CDS
GATCTCATAGTTGATGGTGG+TGG 0.596400 3.4:+23857343 MS.gene86782:CDS
ACTGATATTACTTGTGGACC+AGG 0.614329 3.4:+23858904 MS.gene86782:CDS
AGCATTGGAAGCTTAGGAGG+AGG 0.616219 3.4:+23859056 MS.gene86782:intron
AGAAGCTGAAGTGTCTAATG+TGG 0.616234 3.4:+23859085 MS.gene86782:CDS
ATGGAGTCAGAATAAAGACT+TGG 0.619125 3.4:+23859141 MS.gene86782:CDS
TGAGGCATGTTCTAAAGGGA+AGG 0.623152 3.4:+23857053 MS.gene86782:CDS
TTATTTCACAGCCATGCAAG+GGG 0.654101 3.4:+23857916 MS.gene86782:intron
TCTATGGAACCATCAAAGCA+TGG 0.658530 3.4:+23857260 MS.gene86782:CDS
GATGTGAAATTAACAGCACA+AGG 0.676145 3.4:+23860562 MS.gene86782:CDS
CAAATTGCAGAACTCCGCTG+TGG 0.692865 3.4:+23860432 MS.gene86782:intron
ACAGATCTCATAGTTGATGG+TGG 0.709690 3.4:+23857340 MS.gene86782:CDS
TTCGCCGCAATCTCAAAACA+CGG 0.711068 3.4:+23856642 MS.gene86782:CDS
TATTTCACAGCCATGCAAGG+GGG 0.733319 3.4:+23857917 MS.gene86782:intron
CACATTGCTCAATTCCACAG+CGG 0.766775 3.4:-23860446 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATAAAGTAAAAAAAG+TGG + chr3.4:23859237-23859256 MS.gene86782:intron 10.0%
!! AACTAAAAAATTAAATTAAG+TGG - chr3.4:23859275-23859294 None:intergenic 10.0%
!! ACTAAAAAATTAAATTAAGT+GGG - chr3.4:23859274-23859293 None:intergenic 10.0%
!! GAAAATAAACTAATAATAAA+TGG - chr3.4:23857791-23857810 None:intergenic 10.0%
!!! ATAATGGATTTTTTTTATAA+TGG + chr3.4:23859429-23859448 MS.gene86782:intron 10.0%
!!! TATGGTTTATTTTATAATTT+TGG + chr3.4:23859325-23859344 MS.gene86782:intron 10.0%
!!! TTATTCAATTTAATTTTTCA+TGG + chr3.4:23858006-23858025 MS.gene86782:intron 10.0%
!! AATGTTAATTATTCAAATAG+TGG + chr3.4:23860613-23860632 MS.gene86782:CDS 15.0%
!! AGGTAAATTAAAATGATTTA+TGG + chr3.4:23857157-23857176 MS.gene86782:intron 15.0%
!! ATTAAAATGAATGAAAACAT+GGG - chr3.4:23860219-23860238 None:intergenic 15.0%
!! GAAACTAAACAAATAAAAAA+AGG - chr3.4:23860252-23860271 None:intergenic 15.0%
!! GATTAAATGAATGAATTAAA+AGG - chr3.4:23857450-23857469 None:intergenic 15.0%
!! TACTAATATAATCAAAATCA+TGG + chr3.4:23860408-23860427 MS.gene86782:intron 15.0%
!! TAGTTTAATGAAATGAATTA+AGG + chr3.4:23859205-23859224 MS.gene86782:intron 15.0%
!! TATTAAAATGAATGAAAACA+TGG - chr3.4:23860220-23860239 None:intergenic 15.0%
!! TGAATCAAATTAAAATGATA+AGG - chr3.4:23857859-23857878 None:intergenic 15.0%
!! TTAAAATATTTATGTGATCA+AGG + chr3.4:23856864-23856883 MS.gene86782:intron 15.0%
!!! AATTAGTGATTTGAATTTTA+TGG + chr3.4:23857811-23857830 MS.gene86782:intron 15.0%
!!! AATTAGTTATTTTCTTTACT+TGG - chr3.4:23857643-23857662 None:intergenic 15.0%
!!! ATATTTTTGAGTTATGATTA+TGG - chr3.4:23859921-23859940 None:intergenic 15.0%
!!! ATGAGTTTTATATATTTAAG+TGG - chr3.4:23857199-23857218 None:intergenic 15.0%
!!! ATTTTGGATTTGTTAAATTA+AGG + chr3.4:23857479-23857498 MS.gene86782:intron 15.0%
!!! TAATAAACAAAACATCTTTT+TGG + chr3.4:23858697-23858716 MS.gene86782:intron 15.0%
!!! TATTTTTGAGTTATGATTAT+GGG - chr3.4:23859920-23859939 None:intergenic 15.0%
!! AAACAAATAAAAAAAGGCAA+GGG - chr3.4:23860246-23860265 None:intergenic 20.0%
!! AAACTAAATTGTTCATGTAT+TGG - chr3.4:23856589-23856608 None:intergenic 20.0%
!! AATGAAAAAAAACTCTTTGT+TGG - chr3.4:23859627-23859646 None:intergenic 20.0%
!! ATAATTAACATTGTCACATT+TGG - chr3.4:23860605-23860624 None:intergenic 20.0%
!! ATACTATATATCTAAATGCA+GGG + chr3.4:23859833-23859852 MS.gene86782:intron 20.0%
!! ATTATAGAAATATTGAGGAA+AGG - chr3.4:23857671-23857690 None:intergenic 20.0%
!! ATTGTATTTCAATTGTAAGT+GGG + chr3.4:23858863-23858882 MS.gene86782:CDS 20.0%
!! GATATTTGTTTCCATTTAAA+TGG + chr3.4:23856979-23856998 MS.gene86782:intron 20.0%
!! GTTAAACTTCATGTTAATTT+AGG + chr3.4:23859347-23859366 MS.gene86782:intron 20.0%
!! TAAACAAATAAAAAAAGGCA+AGG - chr3.4:23860247-23860266 None:intergenic 20.0%
!! TAATTATTGTATTGTAGCAT+TGG + chr3.4:23859041-23859060 MS.gene86782:intron 20.0%
!! TGGTTAAAAAAAAATTGATG+CGG + chr3.4:23858992-23859011 MS.gene86782:intron 20.0%
!! TTAATGGAAATGGAAAAATA+TGG + chr3.4:23857371-23857390 MS.gene86782:CDS 20.0%
!! TTTAAAGAGATAAAAAAGAG+TGG - chr3.4:23860331-23860350 None:intergenic 20.0%
!!! ATGAAAATTTAACTTACTGT+TGG - chr3.4:23857945-23857964 None:intergenic 20.0%
!!! ATTTGAGTTAATATCATACA+AGG - chr3.4:23857426-23857445 None:intergenic 20.0%
!!! TATTTTTCCATTTCCATTAA+TGG - chr3.4:23857371-23857390 None:intergenic 20.0%
!!! TCATTGAATTTTGAATTGAT+TGG + chr3.4:23858972-23858991 MS.gene86782:intron 20.0%
!!! TCCTCAATATTTCTATAATT+AGG + chr3.4:23857672-23857691 MS.gene86782:intron 20.0%
!!! TTTTGAAAGATAAACGTTTT+TGG + chr3.4:23859792-23859811 MS.gene86782:intron 20.0%
! AAACTTTAACTAACCAAGTT+TGG + chr3.4:23859492-23859511 MS.gene86782:intron 25.0%
! AAAGGAGAGTAAAGAAATTA+TGG - chr3.4:23857897-23857916 None:intergenic 25.0%
! AAATTAACGAACAAAATCAG+AGG + chr3.4:23860034-23860053 MS.gene86782:intron 25.0%
! AACAAATAAAAAAAGGCAAG+GGG - chr3.4:23860245-23860264 None:intergenic 25.0%
! ATATATTTAAGTGGACATTG+AGG - chr3.4:23857190-23857209 None:intergenic 25.0%
! ATCACTAATCACATTATGAT+TGG + chr3.4:23856905-23856924 MS.gene86782:intron 25.0%
! ATCATAATGTGATTAGTGAT+CGG - chr3.4:23856905-23856924 None:intergenic 25.0%
! ATTTACCTTTAATGCAGTAT+TGG - chr3.4:23857145-23857164 None:intergenic 25.0%
! ATTTGAGAACACCATTTAAA+TGG - chr3.4:23856993-23857012 None:intergenic 25.0%
! CATACTATATATCTAAATGC+AGG + chr3.4:23859832-23859851 MS.gene86782:intron 25.0%
! GAGAAAAAAAAACTTCAACT+AGG - chr3.4:23858954-23858973 None:intergenic 25.0%
! GATTGTATTTCAATTGTAAG+TGG + chr3.4:23858862-23858881 MS.gene86782:CDS 25.0%
! GCCTAATTATAGAAATATTG+AGG - chr3.4:23857676-23857695 None:intergenic 25.0%
! GTTCAAATTAGTTAAGAAGA+AGG - chr3.4:23859605-23859624 None:intergenic 25.0%
! GTTTCATTATGTTAGATCTA+TGG + chr3.4:23857244-23857263 MS.gene86782:intron 25.0%
! TAGTTTACCAAATTATCTCA+AGG + chr3.4:23859290-23859309 MS.gene86782:intron 25.0%
! TATATTTAAGTGGACATTGA+GGG - chr3.4:23857189-23857208 None:intergenic 25.0%
! TATGAAAACATTCTCTGAAT+GGG - chr3.4:23858215-23858234 None:intergenic 25.0%
! TGAATGCAATAATTTGAAAG+TGG + chr3.4:23858063-23858082 MS.gene86782:CDS 25.0%
! TGCATTAGCACTATTATATT+GGG + chr3.4:23859381-23859400 MS.gene86782:intron 25.0%
! TTATGAAAACATTCTCTGAA+TGG - chr3.4:23858216-23858235 None:intergenic 25.0%
! TTGTAAAATCGTGTATTTGT+TGG + chr3.4:23856933-23856952 MS.gene86782:intron 25.0%
! TTTACCTTTAATGCAGTATT+GGG - chr3.4:23857144-23857163 None:intergenic 25.0%
!! ACATGAACAATTTAGTTTCA+TGG + chr3.4:23856591-23856610 MS.gene86782:CDS 25.0%
!! CAAATACACGATTTTACAAT+CGG - chr3.4:23856932-23856951 None:intergenic 25.0%
!! CAAATACACGATTTTACAAT+TGG - chr3.4:23856932-23856951 None:intergenic 25.0%
!! CAATATTTCTATAATTAGGC+TGG + chr3.4:23857676-23857695 MS.gene86782:intron 25.0%
!! TAAAGCCTATAACATTTTCA+GGG + chr3.4:23857109-23857128 MS.gene86782:CDS 25.0%
!! TGTTAATTTAGGACCAAATT+AGG + chr3.4:23859358-23859377 MS.gene86782:intron 25.0%
!! TTGACATGTTTGAAGTAATA+AGG - chr3.4:23859546-23859565 None:intergenic 25.0%
!!! ATTCCTCACTATTTTTGTTA+AGG + chr3.4:23858835-23858854 MS.gene86782:intron 25.0%
!!! ATTTCTCATTTTTGCACTTT+AGG + chr3.4:23857011-23857030 MS.gene86782:intron 25.0%
!!! GGAAAATTTGTTGCATATAT+AGG + chr3.4:23858027-23858046 MS.gene86782:intron 25.0%
!!! TTAAGACAAGAAGTCATTTT+AGG + chr3.4:23858671-23858690 MS.gene86782:intron 25.0%
AAAAAAGAAATGTCAAGAGC+AGG + chr3.4:23859949-23859968 MS.gene86782:CDS 30.0%
AACTTGTCCATCATTAGATT+TGG - chr3.4:23856689-23856708 None:intergenic 30.0%
AATATGAATTCCATCAGTGT+TGG - chr3.4:23858191-23858210 None:intergenic 30.0%
AATGTTACATGGACTCATAA+TGG + chr3.4:23859413-23859432 MS.gene86782:intron 30.0%
AATTTAGCGAAAACAATGTG+TGG + chr3.4:23856720-23856739 MS.gene86782:intron 30.0%
ACAAATAAAAAAAGGCAAGG+GGG - chr3.4:23860244-23860263 None:intergenic 30.0%
ACAAATCCAAAATCAATGCA+CGG - chr3.4:23857472-23857491 None:intergenic 30.0%
AGATGATAGACACTATGTTT+AGG - chr3.4:23857090-23857109 None:intergenic 30.0%
ATATGAATTCCATCAGTGTT+GGG - chr3.4:23858190-23858209 None:intergenic 30.0%
ATGCACAACAAATGCATATA+AGG + chr3.4:23858104-23858123 MS.gene86782:CDS 30.0%
ATGGAAATGGAAAAATATGG+TGG + chr3.4:23857374-23857393 MS.gene86782:CDS 30.0%
ATTATGAGTCCATGTAACAT+TGG - chr3.4:23859414-23859433 None:intergenic 30.0%
CAAATGCATATAAGGATTGA+AGG + chr3.4:23858112-23858131 MS.gene86782:CDS 30.0%
GAAGTAATAAGGTTAGAAAC+TGG - chr3.4:23859535-23859554 None:intergenic 30.0%
GTGATGTACCAAAACATAAA+AGG + chr3.4:23860466-23860485 MS.gene86782:CDS 30.0%
GTGCATTAGCACTATTATAT+TGG + chr3.4:23859380-23859399 MS.gene86782:intron 30.0%
TAGACCTAAAACTCAAGTTT+AGG - chr3.4:23860066-23860085 None:intergenic 30.0%
TCAAGGACTTCAAGTAAATA+TGG + chr3.4:23859307-23859326 MS.gene86782:intron 30.0%
TCACCTTAACAAAAATAGTG+AGG - chr3.4:23858841-23858860 None:intergenic 30.0%
TGGAAATGGAAAAATATGGT+GGG + chr3.4:23857375-23857394 MS.gene86782:CDS 30.0%
TTGAAGTCCTTGAGATAATT+TGG - chr3.4:23859300-23859319 None:intergenic 30.0%
TTGAATGTCACTCTACAAAA+CGG - chr3.4:23856838-23856857 None:intergenic 30.0%
! AACAATTTAGTTTCATGGCA+AGG + chr3.4:23856596-23856615 MS.gene86782:CDS 30.0%
! ACAACCTAAACTTGAGTTTT+AGG + chr3.4:23860059-23860078 MS.gene86782:intron 30.0%
! ACAATTTAGTTTCATGGCAA+GGG + chr3.4:23856597-23856616 MS.gene86782:CDS 30.0%
! ATTTGAAATGTCATGGAATG+AGG + chr3.4:23857035-23857054 MS.gene86782:CDS 30.0%
! CACTTGTTCCTTTTATGTTT+TGG - chr3.4:23860477-23860496 None:intergenic 30.0%
! CTAAAGCCTATAACATTTTC+AGG + chr3.4:23857108-23857127 MS.gene86782:CDS 30.0%
! TTTAGGCATTTGAAATGTCA+TGG + chr3.4:23857028-23857047 MS.gene86782:intron 30.0%
!!! GCTCTTGACATTTCTTTTTT+TGG - chr3.4:23859949-23859968 None:intergenic 30.0%
!!! TTTAATCCGTGCATTGATTT+TGG + chr3.4:23857463-23857482 MS.gene86782:intron 30.0%
AATATTAGGCCATGCTTTGA+TGG - chr3.4:23857272-23857291 None:intergenic 35.0%
AATGAATGAAAACATGGGCT+TGG - chr3.4:23860214-23860233 None:intergenic 35.0%
ACACTAATAGGAACCACTAA+TGG + chr3.4:23859122-23859141 MS.gene86782:CDS 35.0%
AGACTTGGCAGGTAAATTTA+TGG + chr3.4:23859156-23859175 MS.gene86782:intron 35.0%
ATCATCAACCTTACAATGAG+AGG + chr3.4:23856554-23856573 MS.gene86782:CDS 35.0%
ATGGAGTCAGAATAAAGACT+TGG + chr3.4:23859141-23859160 MS.gene86782:CDS 35.0%
ATTAGTGGTTCCTATTAGTG+TGG - chr3.4:23859123-23859142 None:intergenic 35.0%
CAAACCAAATGAGGCTAAAA+TGG - chr3.4:23856494-23856513 None:intergenic 35.0%
CAAATAAAAAAAGGCAAGGG+GGG - chr3.4:23860243-23860262 None:intergenic 35.0%
CTATATATCTAAATGCAGGG+AGG + chr3.4:23859836-23859855 MS.gene86782:intron 35.0%
CTTTATTCTGACTCCATTAG+TGG - chr3.4:23859138-23859157 None:intergenic 35.0%
CTTTGTAAACTTGCCAAACT+TGG - chr3.4:23859508-23859527 None:intergenic 35.0%
GATGTGAAATTAACAGCACA+AGG + chr3.4:23860562-23860581 MS.gene86782:CDS 35.0%
GCTCCATATGCTGAAATATT+AGG - chr3.4:23857286-23857305 None:intergenic 35.0%
TAGTGCTAATGCACCTAATT+TGG - chr3.4:23859374-23859393 None:intergenic 35.0%
TATAGAGCAACACATGATGT+GGG + chr3.4:23860108-23860127 MS.gene86782:intron 35.0%
TATGGAGCCAAATCTAATGA+TGG + chr3.4:23856679-23856698 MS.gene86782:CDS 35.0%
TGAAGGATGCAACAATGTTT+GGG + chr3.4:23858129-23858148 MS.gene86782:CDS 35.0%
TGGCCTAATATTTCAGCATA+TGG + chr3.4:23857280-23857299 MS.gene86782:CDS 35.0%
TTGAAGGATGCAACAATGTT+TGG + chr3.4:23858128-23858147 MS.gene86782:CDS 35.0%
TTGCATGGCTGTGAAATAAA+AGG - chr3.4:23857915-23857934 None:intergenic 35.0%
TTTATTTCACAGCCATGCAA+GGG + chr3.4:23857915-23857934 MS.gene86782:intron 35.0%
! ATCAGTTGCTCGTACATTTT+TGG - chr3.4:23858890-23858909 None:intergenic 35.0%
! TATCTTTTTACCTGTCCCAA+TGG - chr3.4:23858248-23858267 None:intergenic 35.0%
! TATTGTAGCATTGGAAGCTT+AGG + chr3.4:23859050-23859069 MS.gene86782:intron 35.0%
! TTGGCTCTTACTTAGAAGAA+AGG + chr3.4:23856518-23856537 MS.gene86782:CDS 35.0%
!! TGATGGACAAGTTGATAATG+AGG + chr3.4:23856696-23856715 MS.gene86782:CDS 35.0%
!! TTCACCATTTTAGCCTCATT+TGG + chr3.4:23856487-23856506 MS.gene86782:CDS 35.0%
!! TTTTATTTCACAGCCATGCA+AGG + chr3.4:23857914-23857933 MS.gene86782:intron 35.0%
!!! TTAACCGTGTTTTGAGATTG+CGG - chr3.4:23856649-23856668 None:intergenic 35.0%
AACATTGTCACATTTGGCAG+TGG - chr3.4:23860599-23860618 None:intergenic 40.0%
AATCTAATAGTCACAGCACC+AGG + chr3.4:23858157-23858176 MS.gene86782:CDS 40.0%
ACTGATATTACTTGTGGACC+AGG + chr3.4:23858904-23858923 MS.gene86782:CDS 40.0%
AGTCAGAATAAAGACTTGGC+AGG + chr3.4:23859145-23859164 MS.gene86782:CDS 40.0%
ATAGAGCAACACATGATGTG+GGG + chr3.4:23860109-23860128 MS.gene86782:intron 40.0%
ATGGAGCAGATAGAAAGCTT+TGG + chr3.4:23857299-23857318 MS.gene86782:CDS 40.0%
CATGGCCCTGAAAATGTTAT+AGG - chr3.4:23857117-23857136 None:intergenic 40.0%
CCAACCCAATACTGCATTAA+AGG + chr3.4:23857137-23857156 MS.gene86782:CDS 40.0%
CGAGCAACTGATATTACTTG+TGG + chr3.4:23858898-23858917 MS.gene86782:CDS 40.0%
GCAAGTGAATTCCTTTGCAT+GGG - chr3.4:23860540-23860559 None:intergenic 40.0%
GCCAAAACACAAACCAAATG+AGG - chr3.4:23856503-23856522 None:intergenic 40.0%
GGAAGTTGACCAATGTTACA+TGG + chr3.4:23859402-23859421 MS.gene86782:intron 40.0%
GGTGGTACCATTAATGGAAA+TGG + chr3.4:23857361-23857380 MS.gene86782:CDS 40.0%
GTATAGAGCAACACATGATG+TGG + chr3.4:23860107-23860126 MS.gene86782:intron 40.0%
GTCACAGATCTCATAGTTGA+TGG + chr3.4:23857337-23857356 MS.gene86782:CDS 40.0%
TCAACCTTACAATGAGAGGT+TGG + chr3.4:23856558-23856577 MS.gene86782:CDS 40.0%
TCATAACCAACAGCACCATT+GGG + chr3.4:23858230-23858249 MS.gene86782:CDS 40.0%
TCTATGGAACCATCAAAGCA+TGG + chr3.4:23857260-23857279 MS.gene86782:CDS 40.0%
TGCAAGTGAATTCCTTTGCA+TGG - chr3.4:23860541-23860560 None:intergenic 40.0%
TGGAATGAGGCATGTTCTAA+AGG + chr3.4:23857048-23857067 MS.gene86782:CDS 40.0%
TGTGATTAGTGATCGGAACA+AGG - chr3.4:23856898-23856917 None:intergenic 40.0%
TTATTTCACAGCCATGCAAG+GGG + chr3.4:23857916-23857935 MS.gene86782:intron 40.0%
TTCACCAACCTCTCATTGTA+AGG - chr3.4:23856565-23856584 None:intergenic 40.0%
TTCAGAGCAACACAAGATGT+GGG + chr3.4:23860133-23860152 MS.gene86782:intron 40.0%
TTCATAACCAACAGCACCAT+TGG + chr3.4:23858229-23858248 MS.gene86782:CDS 40.0%
TTTCAGAGCAACACAAGATG+TGG + chr3.4:23860132-23860151 MS.gene86782:intron 40.0%
! AGAAGCTGAAGTGTCTAATG+TGG + chr3.4:23859085-23859104 MS.gene86782:CDS 40.0%
! CCTTTAATGCAGTATTGGGT+TGG - chr3.4:23857140-23857159 None:intergenic 40.0%
! GGAATGAGGCATGTTCTAAA+GGG + chr3.4:23857049-23857068 MS.gene86782:CDS 40.0%
! TTCTAAAGGGAAGGTTCTTG+TGG + chr3.4:23857062-23857081 MS.gene86782:CDS 40.0%
!! ACAGATCTCATAGTTGATGG+TGG + chr3.4:23857340-23857359 MS.gene86782:CDS 40.0%
!!! GCCTCATTTGGTTTGTGTTT+TGG + chr3.4:23856499-23856518 MS.gene86782:CDS 40.0%
ACGGTTAACGTTGACGACTA+TGG + chr3.4:23856661-23856680 MS.gene86782:CDS 45.0%
ATCTAAATGCAGGGAGGTTC+TGG + chr3.4:23859842-23859861 MS.gene86782:intron 45.0%
ATGAACCTGATTCCATGTCC+TGG - chr3.4:23858925-23858944 None:intergenic 45.0%
ATTACTTGTGGACCAGGACA+TGG + chr3.4:23858910-23858929 MS.gene86782:CDS 45.0%
GTGAACAGAGCCACACTAAT+AGG + chr3.4:23859110-23859129 MS.gene86782:CDS 45.0%
TATTTCACAGCCATGCAAGG+GGG + chr3.4:23857917-23857936 MS.gene86782:intron 45.0%
TCAGAGCAACACAAGATGTG+GGG + chr3.4:23860134-23860153 MS.gene86782:intron 45.0%
TTCGCCGCAATCTCAAAACA+CGG + chr3.4:23856642-23856661 MS.gene86782:CDS 45.0%
! TGTAGCATTGGAAGCTTAGG+AGG + chr3.4:23859053-23859072 MS.gene86782:intron 45.0%
!! AATGCAGTATTGGGTTGGCA+TGG - chr3.4:23857135-23857154 None:intergenic 45.0%
!! GATCTCATAGTTGATGGTGG+TGG + chr3.4:23857343-23857362 MS.gene86782:CDS 45.0%
!! GATGGTGGTGGTACCATTAA+TGG + chr3.4:23857355-23857374 MS.gene86782:CDS 45.0%
!! TGAGGCATGTTCTAAAGGGA+AGG + chr3.4:23857053-23857072 MS.gene86782:CDS 45.0%
!! AAATTTATTTACAAATTAAA+AGG - chr3.4:23857702-23857721 None:intergenic 5.0%
!! AACTATTTATTATTATTTAT+TGG - chr3.4:23858760-23858779 None:intergenic 5.0%
!! ACTATTTATTATTATTTATT+GGG - chr3.4:23858759-23858778 None:intergenic 5.0%
!!! TTAATTTTTTTTTAATTGAT+TGG + chr3.4:23859018-23859037 MS.gene86782:intron 5.0%
!!! TTATTATTTTTTTTTTTTGA+GGG - chr3.4:23858783-23858802 None:intergenic 5.0%
!!! TTTATTATTTTTTTTTTTTG+AGG - chr3.4:23858784-23858803 None:intergenic 5.0%
AGCAAAGCTGTCCCATGCAA+AGG + chr3.4:23860526-23860545 MS.gene86782:CDS 50.0%
CAGAGCAACACAAGATGTGG+GGG + chr3.4:23860135-23860154 MS.gene86782:intron 50.0%
! AGCATTGGAAGCTTAGGAGG+AGG + chr3.4:23859056-23859075 MS.gene86782:intron 50.0%
!!! GTGTTTTGAGATTGCGGCGA+AGG - chr3.4:23856643-23856662 None:intergenic 50.0%
ACCAACAGCACCATTGGGAC+AGG + chr3.4:23858235-23858254 MS.gene86782:CDS 55.0%
GGACACAGTCCCAACACTGA+TGG + chr3.4:23858178-23858197 MS.gene86782:CDS 55.0%
GTGGACCAGGACATGGAATC+AGG + chr3.4:23858917-23858936 MS.gene86782:CDS 55.0%
! TCAGTGTTGGGACTGTGTCC+TGG - chr3.4:23858178-23858197 None:intergenic 55.0%
!! ACCTGTCCCAATGGTGCTGT+TGG - chr3.4:23858239-23858258 None:intergenic 55.0%
! ACTGTTGGTGCCCCCTTGCA+TGG - chr3.4:23857930-23857949 None:intergenic 60.0%
! TGCGGCGAAGGCGCGATAGA+TGG - chr3.4:23856631-23856650 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 23856448 23860663 23856448 ID=MS.gene86782
chr3.4 mRNA 23856448 23860663 23856448 ID=MS.gene86782.t1;Parent=MS.gene86782
chr3.4 exon 23856448 23856717 23856448 ID=MS.gene86782.t1.exon1;Parent=MS.gene86782.t1
chr3.4 CDS 23856448 23856717 23856448 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23857033 23857158 23857033 ID=MS.gene86782.t1.exon2;Parent=MS.gene86782.t1
chr3.4 CDS 23857033 23857158 23857033 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23857259 23857426 23857259 ID=MS.gene86782.t1.exon3;Parent=MS.gene86782.t1
chr3.4 CDS 23857259 23857426 23857259 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23857927 23857947 23857927 ID=MS.gene86782.t1.exon4;Parent=MS.gene86782.t1
chr3.4 CDS 23857927 23857947 23857927 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23858049 23858256 23858049 ID=MS.gene86782.t1.exon5;Parent=MS.gene86782.t1
chr3.4 CDS 23858049 23858256 23858049 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23858857 23858938 23858857 ID=MS.gene86782.t1.exon6;Parent=MS.gene86782.t1
chr3.4 CDS 23858857 23858938 23858857 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23859058 23859166 23859058 ID=MS.gene86782.t1.exon7;Parent=MS.gene86782.t1
chr3.4 CDS 23859058 23859166 23859058 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23859854 23859970 23859854 ID=MS.gene86782.t1.exon8;Parent=MS.gene86782.t1
chr3.4 CDS 23859854 23859970 23859854 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
chr3.4 exon 23860442 23860663 23860442 ID=MS.gene86782.t1.exon9;Parent=MS.gene86782.t1
chr3.4 CDS 23860442 23860663 23860442 ID=cds.MS.gene86782.t1;Parent=MS.gene86782.t1
Gene Sequence

>MS.gene86782

ATGAGAAAGATGTATTCACAAAGCATAATTCTTTCACTTTTCACCATTTTAGCCTCATTTGGTTTGTGTTTTGGCTCTTACTTAGAAGAAAGGCATTATTTAACAAATCATCAACCTTACAATGAGAGGTTGGTGAAGCCAATACATGAACAATTTAGTTTCATGGCAAGGGCTCATCATCCATCTATCGCGCCTTCGCCGCAATCTCAAAACACGGTTAACGTTGACGACTATGGAGCCAAATCTAATGATGGACAAGTTGATAATGAGGTAATTTAGCGAAAACAATGTGTGGTATATTTTTACTGAAGTTGTATTTGAATCTCACGTTGTCATGATTCATGAGTTATGCAATGCTTATATGTGTTTTTGTCTCATCTTACAAGTCCGTTTTGTAGAGTGACATTCAAATTCATTTAAAATATTTATGTGATCAAGGTTTAACTGCCTTGTTCCGATCACTAATCACATTATGATTGGTCCAATTGTAAAATCGTGTATTTGTTGGTCCGATTGTAAAATCGTGTATTTGATATTTGTTTCCATTTAAATGGTGTTCTCAAATTTCTCATTTTTGCACTTTAGGCATTTGAAATGTCATGGAATGAGGCATGTTCTAAAGGGAAGGTTCTTGTGGTACCTAAACATAGTGTCTATCATCTAAAGCCTATAACATTTTCAGGGCCATGCCAACCCAATACTGCATTAAAGGTAAATTAAAATGATTTATGGTTATAACCCTCAATGTCCACTTAAATATATAAAACTCATTGTAACGTTTTTGTGTTTAATTACTGTTTCATTATGTTAGATCTATGGAACCATCAAAGCATGGCCTAATATTTCAGCATATGGAGCAGATAGAAAGCTTTGGATCAAGTTTGAGAATGTCACAGATCTCATAGTTGATGGTGGTGGTACCATTAATGGAAATGGAAAAATATGGTGGGAGAATTCTTGCAAAGTGAATGAAAGCCTTGTATGATATTAACTCAAATTCCTTTTAATTCATTCATTTAATCCGTGCATTGATTTTGGATTTGTTAAATTAAGGAAAAATATATTTTGTCATTTTTTGTTTCTTGTTTACTTTTTAGTATTAACTAAAAATCGTATACTATTATACACTTTAATTTTTTTCTTTTCAAAAATTTATTTTGTTTATACAAACATAAAGAAAATAACTTCTTTGCCAAGTAAAGAAAATAACTAATTATTCACCTTTCCTCAATATTTCTATAATTAGGCTGGCCTTTTAATTTGTAAATAAATTTTTTTTACAAATAAAGAAAATAACTTACAAAGAAACATAAAGAATTGTTTTTAAGAAATTTTAATAACCATTTATTATTAGTTTATTTTCAATTAGTGATTTGAATTTTATGGAATCATTATATAGACTTTTTTTCCTTATCATTTTAATTTGATTCAAATTTTAAATACTGACCATAATTTCTTTACTCTCCTTTTATTTCACAGCCATGCAAGGGGGCACCAACAGTAAGTTAAATTTTCATTTTTGTGAACTTTATTACATGCTTTTCTGAAATACTCATCTTTATTCAATTTAATTTTTCATGGAAAATTTGTTGCATATATAGGCTGTGACTTTCGATGAATGCAATAATTTGAAAGTGGATAAGATAAGATTCAGAAATGCACAACAAATGCATATAAGGATTGAAGGATGCAACAATGTTTGGGCATCAAATCTAATAGTCACAGCACCAGGACACAGTCCCAACACTGATGGAATTCATATTACCCATTCAGAGAATGTTTTCATAACCAACAGCACCATTGGGACAGGTAAAAAGATATATAAATGAAAAAAAATGTCACATTATAATATCATTTTTCATAATTTATGTGTTTTCATATTATAATATCATTTTTTTTAAAATCATAACTAATTATATGATGAAAAATTATTAGACGTTATAGTAGAGTAATTTTGCATTGTTTGTGCATAATAATTAAGTAAATCAAAGATTATTAGATTATGCACAATTTATTTATTTTTTTAAAGAATTATGCACAATTTCTAAACAAATTATTCAAAATGTATAATTTTTCTTAAAAAATATGTAGCATTTGTATTTTCTTTAAATGACGCACATATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTACTCTAATCATTAAAAATGAATTATTATTTTTATTTTTATAGAAACTAACTTTAAGACAAGAAGTCATTTTAGGAAGTAATAAACAAAACATCTTTTTGGTAGTGAATAAACAAAACATCTATTATAACTTTATTACCCAATAAATAATAATAAATAGTTCCCTCAAAAAAAAAAAATAATAAATAGTTACTTGAATTTTTCAATTTTCTAATTTATTCCTCACTATTTTTGTTAAGGTGATGATTGTATTTCAATTGTAAGTGGGTCCAAAAATGTACGAGCAACTGATATTACTTGTGGACCAGGACATGGAATCAGGTTCATGACTCTCCTAGTTGAAGTTTTTTTTTCTCATTGAATTTTGAATTGATTGGTTAAAAAAAAATTGATGCGGTAATTAATTTTTTTTTAATTGATTGGTAATTATTGTATTGTAGCATTGGAAGCTTAGGAGGAGGTAACACAGAAGCTGAAGTGTCTAATGTGGAAGTGAACAGAGCCACACTAATAGGAACCACTAATGGAGTCAGAATAAAGACTTGGCAGGTAAATTTATGGCAATTAAACACTCTACAACTTTGCAATAGTTTAATGAAATGAATTAAGGCTCATTTATAAATTATAAAGTAAAAAAAGTGGAATTGATGCTACCCACTTAATTTAATTTTTTAGTTTACCAAATTATCTCAAGGACTTCAAGTAAATATGGTTTATTTTATAATTTTGGTTAAACTTCATGTTAATTTAGGACCAAATTAGGTGCATTAGCACTATTATATTGGGAAGTTGACCAATGTTACATGGACTCATAATGGATTTTTTTTATAATGGTTGATTTTCATTTCTAACTAAACATAAAGTTTGACAAGCAAAACTTTAACTAACCAAGTTTGGCAAGTTTACAAAGTCTACCAGTTTCTAACCTTATTACTTCAAACATGTCAATAACATTTTATATTGTGTTGTTCAACTTTCTTTAGTCCTTCTTCTTAACTAATTTGAACCAACAAAGAGTTTTTTTTCATTTTTTTTTTTTATAATTTTAGATGTGCATATACTTGATGAACATTAACTTTGACTAGACTATAAGAATGTATTTAAAAAAATTGATAAAAATTTATTCATTGTTATATTAGTAGTGTAGAGCTTAACTTGTTAGCTTTACTATTGATTTTGAAAGATAAACGTTTTTGGTCTAATGTCTTATTTTTCATACTATATATCTAAATGCAGGGAGGTTCTGGCTATGCAAGAAACATCAAATTTATGAACATAGTCATGCGAAATGTGACTAATCCCATAATCATAACTCAAAAATATTGTGACCAAAAAAAGAAATGTCAAGAGCAGGTAATTGTTTTTAAATGTCTTAACTACTTTTATTTTTGATATTATTTGAAGAAGAAAAAAATAAAATTAACGAACAAAATCAGAGGAAACAACCTAAACTTGAGTTTTAGGTCTAAAAACAAATTATCAAAAATATGTATAGAGCAACACATGATGTGGGGTTTCAGAGCAACACAAGATGTGGGGGTATAAAGAGCAATTATCTATAAAAAATTCTTTTTCATTTTACAAATGTTTTCACCAAGCCCATGTTTTCATTCATTTTAATACCCCCCTTGCCTTTTTTTATTTGTTTAGTTTCATGATTCAAAGTTCAAACTACAATTTATGCTTTTATAAAATTTGACTGCGTTTAGTCCACTCTTTTTTATCTCTTTAAATATTTGTTACTAATTTAATTTTTTTAAAATGTAGTTATAATACTGTTTAAAATTCGTTACTAATATAATCAAAATCATGGTCAAATTGCAGAACTCCGCTGTGGAATTGAGCAATGTGATGTACCAAAACATAAAAGGAACAAGTGCTTCAGAAGTTGCAATCAAACTTGAATGTAGCAAAGCTGTCCCATGCAAAGGAATTCACTTGCAAGATGTGAAATTAACAGCACAAGGCAATGATAACACCACTGCCAAATGTGACAATGTTAATTATTCAAATAGTGGAATGTTCTTTCCTAAGTGTTACCCATGA
Protein sequence

>MS.gene86782.t1

MRKMYSQSIILSLFTILASFGLCFGSYLEERHYLTNHQPYNERLVKPIHEQFSFMARAHHPSIAPSPQSQNTVNVDDYGAKSNDGQVDNEAFEMSWNEACSKGKVLVVPKHSVYHLKPITFSGPCQPNTALKIYGTIKAWPNISAYGADRKLWIKFENVTDLIVDGGGTINGNGKIWWENSCKVNESLPCKGAPTAVTFDECNNLKVDKIRFRNAQQMHIRIEGCNNVWASNLIVTAPGHSPNTDGIHITHSENVFITNSTIGTGDDCISIVSGSKNVRATDITCGPGHGISIGSLGGGNTEAEVSNVEVNRATLIGTTNGVRIKTWQGGSGYARNIKFMNIVMRNVTNPIIITQKYCDQKKKCQEQNSAVELSNVMYQNIKGTSASEVAIKLECSKAVPCKGIHLQDVKLTAQGNDNTTAKCDNVNYSNSGMFFPKCYP