AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86922

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86922.t1 MTR_1g106210 93.141 277 3 3 1 277 1 261 1.75e-180 497
MS.gene86922.t1 MTR_5g005040 39.333 150 63 5 125 272 109 232 3.38e-18 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86922.t1 AT3G19650 31.731 312 153 9 7 277 10 302 1.64e-16 78.2

Find 66 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCCTTTCATTCCTTTC+TGG 0.052010 1.2:-79187396 None:intergenic
CCATCTCTTCTATATTTCTT+AGG 0.105254 1.2:-79185302 None:intergenic
ATCTGATCATCCTCATTCTT+TGG 0.243825 1.2:-79187666 None:intergenic
TGAGGAAAAGGCCAGCATTT+AGG 0.247990 1.2:+79187292 MS.gene86922:CDS
TTTCTGGAGGGTGGCTAGTA+TGG 0.266311 1.2:-79187380 None:intergenic
TGAGAAATCACTGGCTAAAC+TGG 0.294321 1.2:+79187209 MS.gene86922:CDS
GAGGAAAAGGCCAGCATTTA+GGG 0.296936 1.2:+79187293 MS.gene86922:CDS
TTCTCTGTTTGAGTCTTAAT+AGG 0.302168 1.2:-79187600 None:intergenic
CTTCCTAGGTTCTCGCTTGT+TGG 0.303209 1.2:-79187484 None:intergenic
CATCTCTTCTATATTTCTTA+GGG 0.330904 1.2:-79185301 None:intergenic
AGAGGAAGAGACAAATTTAA+TGG 0.334230 1.2:+79187561 MS.gene86922:CDS
TTTGACCTGTCTCCAACCTT+TGG 0.360011 1.2:-79187170 None:intergenic
GCTTCTCAGGTCTATCTAGA+TGG 0.381632 1.2:-79187440 None:intergenic
TCCTCTGCAATTCGCTTCTC+AGG 0.409939 1.2:-79187453 None:intergenic
TCAGGTCTATCTAGATGGTT+AGG 0.410188 1.2:-79187435 None:intergenic
TGATAATAAGAGCGAGATCT+TGG 0.413507 1.2:-79185407 None:intergenic
AAGATCTCGCTCTTATTATC+AGG 0.426217 1.2:+79185409 MS.gene86922:CDS
GATGAAAATCCATCCTTCCT+AGG 0.435435 1.2:-79187498 None:intergenic
TCCTTTCATTCCTTTCTGGA+GGG 0.447281 1.2:-79187392 None:intergenic
TGGAGGTTGAGATGAATGTT+TGG 0.448773 1.2:-79185387 None:intergenic
GGATGATCAGATTGCAAAGC+TGG 0.457045 1.2:+79187677 MS.gene86922:CDS
TTCCTTTCATTCCTTTCTGG+AGG 0.457901 1.2:-79187393 None:intergenic
CAACACCTTCTGTGAGGAAA+AGG 0.461132 1.2:+79187280 MS.gene86922:CDS
TTTACTGTCACTGTCGCAGC+TGG 0.462931 1.2:-79187351 None:intergenic
GAAGCGAATTGCAGAGGATA+GGG 0.477705 1.2:+79187458 MS.gene86922:CDS
TGGTTCAGAATGATCATTTG+TGG 0.481130 1.2:-79185360 None:intergenic
ATCTTCTTCTCCCTAAATGC+TGG 0.485997 1.2:-79187303 None:intergenic
AAAGGTATATGCTCAAAGTA+AGG 0.495225 1.2:+79187140 MS.gene86922:CDS
AGAATGACGACGCTTCGAAT+CGG 0.503307 1.2:-79185175 None:intergenic
TTTCATTCCTTTCTGGAGGG+TGG 0.506208 1.2:-79187389 None:intergenic
TTTCATCAAGAGTAAGGTAT+GGG 0.512420 1.2:+79187514 MS.gene86922:CDS
TGGTCGAAGCACTGGTCAAA+GGG 0.514223 1.2:+79186991 MS.gene86922:CDS
GTGCAGGTTGGTCGAAGCAC+TGG 0.521659 1.2:+79186983 MS.gene86922:CDS
CCTAAGAAATATAGAAGAGA+TGG 0.536031 1.2:+79185302 MS.gene86922:CDS
TAAGGAGAATAACCAAAGGT+TGG 0.542846 1.2:+79187158 MS.gene86922:CDS
AATAACCAAAGGTTGGAGAC+AGG 0.542889 1.2:+79187165 MS.gene86922:CDS
TTGGTCGAAGCACTGGTCAA+AGG 0.551358 1.2:+79186990 MS.gene86922:CDS
TTAAGACTCAAACAGAGAAG+TGG 0.554278 1.2:+79187604 MS.gene86922:CDS
TAATAAGAGCGAGATCTTGG+AGG 0.562376 1.2:-79185404 None:intergenic
GCAAAGAGATGAGAAATCAC+TGG 0.563837 1.2:+79187200 MS.gene86922:CDS
AAGCGAGAACCTAGGAAGGA+TGG 0.574352 1.2:+79187489 MS.gene86922:CDS
ACCCTCCAGAAAGGAATGAA+AGG 0.576042 1.2:+79187391 MS.gene86922:CDS
TCCAGAAAGGAATGAAAGGA+AGG 0.580588 1.2:+79187395 MS.gene86922:CDS
ATACTAGCCACCCTCCAGAA+AGG 0.585699 1.2:+79187382 MS.gene86922:CDS
GAAACATGATTTGTATCAAG+AGG 0.586742 1.2:+79187626 MS.gene86922:CDS
AGAAGCGAATTGCAGAGGAT+AGG 0.587397 1.2:+79187457 MS.gene86922:CDS
AAATCACTGGCTAAACTGGA+TGG 0.588753 1.2:+79187213 MS.gene86922:CDS
AGATTCTATTCCAAAGAATG+AGG 0.590814 1.2:+79187656 MS.gene86922:CDS
ATGACGACGCTTCGAATCGG+AGG 0.593613 1.2:-79185172 None:intergenic
ACCTGAGAAGCGAATTGCAG+AGG 0.605860 1.2:+79187452 MS.gene86922:CDS
TGAATGGGGCAATACTGTGC+AGG 0.607456 1.2:+79186967 MS.gene86922:CDS
TTCATCAAGAGTAAGGTATG+GGG 0.620262 1.2:+79187515 MS.gene86922:CDS
CAACAAGCGAGAACCTAGGA+AGG 0.622024 1.2:+79187485 MS.gene86922:CDS
GATCTCAGTGGATTCAAGAG+AGG 0.625358 1.2:+79187323 MS.gene86922:CDS
AGAAAGGAATGAAAGGAAGG+AGG 0.636511 1.2:+79187398 MS.gene86922:CDS
TCATCAAGAGTAAGGTATGG+GGG 0.637056 1.2:+79187516 MS.gene86922:CDS
AATCCAACAAGCGAGAACCT+AGG 0.638185 1.2:+79187481 MS.gene86922:CDS
ACTGGTCAAAGGGAAGCTGA+TGG 0.653810 1.2:+79187001 MS.gene86922:CDS
GGTTGAGATGAATGTTTGGA+CGG 0.657350 1.2:-79185383 None:intergenic
CTAAGCTCGATCGTGAACCA+AGG 0.670920 1.2:+79185196 MS.gene86922:CDS
TGGGGCAATACTGTGCAGGT+TGG 0.672427 1.2:+79186971 MS.gene86922:CDS
AAAGTAAGGAGAATAACCAA+AGG 0.679155 1.2:+79187154 MS.gene86922:CDS
TGAGATGAATGTTTGGACGG+TGG 0.681415 1.2:-79185380 None:intergenic
ATGATCCAACACCTTCTGTG+AGG 0.687855 1.2:+79187274 MS.gene86922:CDS
TAGGGAGAAGAAGATCTCAG+TGG 0.726006 1.2:+79187311 MS.gene86922:CDS
GGAGGCAATAGCAATCACAG+AGG 0.738956 1.2:+79187543 MS.gene86922:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCATTTATTTTTAAACAA+AGG - chr1.2:79186155-79186174 None:intergenic 10.0%
!!! AATCATTTTATTTAAATGTT+AGG + chr1.2:79185871-79185890 MS.gene86922:intron 10.0%
!!! ATCATTTTATTTAAATGTTA+GGG + chr1.2:79185872-79185891 MS.gene86922:intron 10.0%
!!! TGATATATATTATTTTCTTT+TGG + chr1.2:79187045-79187064 MS.gene86922:intron 10.0%
!! TAAATGTTAATAAAAACAAC+TGG + chr1.2:79186588-79186607 MS.gene86922:intron 15.0%
!!! GTTGTTTTTATTAACATTTA+TGG - chr1.2:79186588-79186607 None:intergenic 15.0%
!! AACATCTATAGCATATAAAA+AGG - chr1.2:79186842-79186861 None:intergenic 20.0%
!! ACATCTATAGCATATAAAAA+GGG - chr1.2:79186841-79186860 None:intergenic 20.0%
!! GATTTAGATGATTGATTATT+TGG + chr1.2:79185243-79185262 MS.gene86922:intron 20.0%
!! TACCTTTATATAAACAGTAT+TGG + chr1.2:79186747-79186766 MS.gene86922:intron 20.0%
!! TTAATGTGCTTCTTTATATT+TGG + chr1.2:79185463-79185482 MS.gene86922:intron 20.0%
!! TTCCAATACTGTTTATATAA+AGG - chr1.2:79186752-79186771 None:intergenic 20.0%
! AAAACCAATCTCAACATTTA+TGG - chr1.2:79186679-79186698 None:intergenic 25.0%
! ACCCAATACAACAATATATA+CGG - chr1.2:79186555-79186574 None:intergenic 25.0%
! ATGCAAATTTGAATGTAGTT+AGG - chr1.2:79185502-79185521 None:intergenic 25.0%
! CATCTCTTCTATATTTCTTA+GGG - chr1.2:79185304-79185323 None:intergenic 25.0%
! CTAAATCAAGTAGTTAACTT+GGG + chr1.2:79186261-79186280 MS.gene86922:intron 25.0%
! TAATCAATCATCTAAATCCA+TGG - chr1.2:79185241-79185260 None:intergenic 25.0%
! TAGGATTTGCATTATGATTA+AGG - chr1.2:79186453-79186472 None:intergenic 25.0%
! TCTAAATCAAGTAGTTAACT+TGG + chr1.2:79186260-79186279 MS.gene86922:intron 25.0%
! TGGTAAACAATAAATACGTT+GGG + chr1.2:79186338-79186357 MS.gene86922:intron 25.0%
! TTGGAAAGAAAACATCTTAA+TGG + chr1.2:79186766-79186785 MS.gene86922:intron 25.0%
! TTGTCAATAGCATATTATAG+CGG + chr1.2:79185729-79185748 MS.gene86922:intron 25.0%
! TTTATTGTATATGGATGGTT+TGG + chr1.2:79187069-79187088 MS.gene86922:intron 25.0%
!!! GAATTTTTTATCTCATTCTG+AGG - chr1.2:79185846-79185865 None:intergenic 25.0%
!!! GTTTTCCAACATTTTAAGTT+TGG - chr1.2:79185705-79185724 None:intergenic 25.0%
!!! TAAGTGTGTGTTTGTTTTAT+CGG - chr1.2:79186068-79186087 None:intergenic 25.0%
!!! TCTTTTGGCTTTATTGTATA+TGG + chr1.2:79187060-79187079 MS.gene86922:intron 25.0%
!!! TGGTTTTTGAATGTTGATTA+TGG + chr1.2:79185263-79185282 MS.gene86922:intron 25.0%
!!! TGTATGCAAACTATTTTTCT+GGG + chr1.2:79186868-79186887 MS.gene86922:intron 25.0%
AAAGTAAGGAGAATAACCAA+AGG + chr1.2:79187154-79187173 MS.gene86922:CDS 30.0%
AATCTCAACATTTATGGTGA+AGG - chr1.2:79186673-79186692 None:intergenic 30.0%
AATGGACAATGAAGATAACT+TGG + chr1.2:79186784-79186803 MS.gene86922:intron 30.0%
ACATCCATTTATGCAATGTT+TGG + chr1.2:79185933-79185952 MS.gene86922:intron 30.0%
AGAGGAAGAGACAAATTTAA+TGG + chr1.2:79187561-79187580 MS.gene86922:CDS 30.0%
AGATTCTATTCCAAAGAATG+AGG + chr1.2:79187656-79187675 MS.gene86922:CDS 30.0%
ATGTCCAAACATTGCATAAA+TGG - chr1.2:79185940-79185959 None:intergenic 30.0%
CCAAACCAAACTTAAAATGT+TGG + chr1.2:79185697-79185716 MS.gene86922:intron 30.0%
CCATCTCTTCTATATTTCTT+AGG - chr1.2:79185305-79185324 None:intergenic 30.0%
CCTAAGAAATATAGAAGAGA+TGG + chr1.2:79185302-79185321 MS.gene86922:CDS 30.0%
CTGGTAAACAATAAATACGT+TGG + chr1.2:79186337-79186356 MS.gene86922:intron 30.0%
GCCGTATATATTGTTGTATT+GGG + chr1.2:79186551-79186570 MS.gene86922:intron 30.0%
TAAACAATAAATACGTTGGG+AGG + chr1.2:79186341-79186360 MS.gene86922:intron 30.0%
TAACATCGAGAAAATGGAAA+TGG - chr1.2:79186478-79186497 None:intergenic 30.0%
TGATTTCTCTATTCACAAAG+AGG + chr1.2:79186170-79186189 MS.gene86922:intron 30.0%
TGCCGTATATATTGTTGTAT+TGG + chr1.2:79186550-79186569 MS.gene86922:intron 30.0%
TTATCGGTGGAATAAACAAA+CGG - chr1.2:79186052-79186071 None:intergenic 30.0%
TTCACCATAAATGTTGAGAT+TGG + chr1.2:79186672-79186691 MS.gene86922:intron 30.0%
TTCTCTGTTTGAGTCTTAAT+AGG - chr1.2:79187603-79187622 None:intergenic 30.0%
TTTCATCAAGAGTAAGGTAT+GGG + chr1.2:79187514-79187533 MS.gene86922:CDS 30.0%
! AAAGGTATATGCTCAAAGTA+AGG + chr1.2:79187140-79187159 MS.gene86922:CDS 30.0%
! ACTACTTGATTTAGAGCTAA+AGG - chr1.2:79186255-79186274 None:intergenic 30.0%
! ATGGATTTTCATCAAGAGTA+AGG + chr1.2:79187508-79187527 MS.gene86922:CDS 30.0%
! GAAACATGATTTGTATCAAG+AGG + chr1.2:79187626-79187645 MS.gene86922:CDS 30.0%
! TTTTAACAGCATCATGAATG+GGG + chr1.2:79186953-79186972 MS.gene86922:intron 30.0%
! TTTTCATCAAGAGTAAGGTA+TGG + chr1.2:79187513-79187532 MS.gene86922:CDS 30.0%
!! ATGTAGCTTCTTTAAAACCA+CGG - chr1.2:79186002-79186021 None:intergenic 30.0%
!! GTATGCAAACTATTTTTCTG+GGG + chr1.2:79186869-79186888 MS.gene86922:intron 30.0%
!! GTGTATGCAAACTATTTTTC+TGG + chr1.2:79186867-79186886 MS.gene86922:intron 30.0%
!! GTTGATAACATTGTTGGAAA+AGG + chr1.2:79186109-79186128 MS.gene86922:intron 30.0%
!! GTTTGGAATCGAAGAATTTT+GGG - chr1.2:79187117-79187136 None:intergenic 30.0%
!! GTTTTAACAGCATCATGAAT+GGG + chr1.2:79186952-79186971 MS.gene86922:intron 30.0%
!! TGTTTGGAATCGAAGAATTT+TGG - chr1.2:79187118-79187137 None:intergenic 30.0%
!! TTGGCTTTATTGTATATGGA+TGG + chr1.2:79187064-79187083 MS.gene86922:intron 30.0%
!!! CCAACATTTTAAGTTTGGTT+TGG - chr1.2:79185700-79185719 None:intergenic 30.0%
!!! TGTTTTAACAGCATCATGAA+TGG + chr1.2:79186951-79186970 MS.gene86922:intron 30.0%
AACAATATATACGGCAATCC+TGG - chr1.2:79186546-79186565 None:intergenic 35.0%
AAGATCTCGCTCTTATTATC+AGG + chr1.2:79185409-79185428 MS.gene86922:CDS 35.0%
ACTGACCTCCTTATAAATCA+AGG - chr1.2:79186714-79186733 None:intergenic 35.0%
AGTAGCTAACATCGAGAAAA+TGG - chr1.2:79186484-79186503 None:intergenic 35.0%
ATCTGATCATCCTCATTCTT+TGG - chr1.2:79187669-79187688 None:intergenic 35.0%
ATGTAGTTAGGAACAGAAGT+TGG - chr1.2:79185490-79185509 None:intergenic 35.0%
ATGTTAGCTACTACAACATG+TGG + chr1.2:79186492-79186511 MS.gene86922:intron 35.0%
CTGACCTCCTTATAAATCAA+GGG - chr1.2:79186713-79186732 None:intergenic 35.0%
GATGCTGTTAAAACATGCAT+TGG - chr1.2:79186946-79186965 None:intergenic 35.0%
GTTACTTATTCGAGATTACC+AGG + chr1.2:79186525-79186544 MS.gene86922:intron 35.0%
TAAATCCATGGAACTAGAAG+AGG - chr1.2:79185229-79185248 None:intergenic 35.0%
TAAATGGATGTCTCCCTAAA+TGG - chr1.2:79185924-79185943 None:intergenic 35.0%
TAAGGAGAATAACCAAAGGT+TGG + chr1.2:79187158-79187177 MS.gene86922:CDS 35.0%
TCTTCGATTCCAAACAGAAA+AGG + chr1.2:79187122-79187141 MS.gene86922:intron 35.0%
TGACCTCCTTATAAATCAAG+GGG - chr1.2:79186712-79186731 None:intergenic 35.0%
TGATAATAAGAGCGAGATCT+TGG - chr1.2:79185410-79185429 None:intergenic 35.0%
TGGTTCAGAATGATCATTTG+TGG - chr1.2:79185363-79185382 None:intergenic 35.0%
TGTATATCCCTGCATACAAT+TGG + chr1.2:79186300-79186319 MS.gene86922:intron 35.0%
TTAAGACTCAAACAGAGAAG+TGG + chr1.2:79187604-79187623 MS.gene86922:CDS 35.0%
TTATGCAATGTTTGGACATG+TGG + chr1.2:79185941-79185960 MS.gene86922:intron 35.0%
TTCAGATCTCACTCCATTTA+GGG + chr1.2:79185908-79185927 MS.gene86922:intron 35.0%
TTCATCAAGAGTAAGGTATG+GGG + chr1.2:79187515-79187534 MS.gene86922:CDS 35.0%
TTTCAGATCTCACTCCATTT+AGG + chr1.2:79185907-79185926 MS.gene86922:intron 35.0%
! ACAACTGGAATTTGCTTTGA+CGG + chr1.2:79186603-79186622 MS.gene86922:intron 35.0%
! GAGCATATACCTTTTCTGTT+TGG - chr1.2:79187134-79187153 None:intergenic 35.0%
! GGTAATTTTCAGAGAAGAGA+CGG + chr1.2:79187234-79187253 MS.gene86922:CDS 35.0%
!!! TTTTATCTCATTCTGAGGTG+AGG - chr1.2:79185841-79185860 None:intergenic 35.0%
AAATACGTTGGGAGGAAACT+GGG + chr1.2:79186349-79186368 MS.gene86922:intron 40.0%
AAGCTGACCAATTGTATGCA+GGG - chr1.2:79186310-79186329 None:intergenic 40.0%
AATAACCAAAGGTTGGAGAC+AGG + chr1.2:79187165-79187184 MS.gene86922:CDS 40.0%
AATTGCTCACAACTTGTACC+AGG - chr1.2:79186424-79186443 None:intergenic 40.0%
AGAAAGGAATGAAAGGAAGG+AGG + chr1.2:79187398-79187417 MS.gene86922:CDS 40.0%
AGTAGAGCAACCTATTGTAG+CGG + chr1.2:79185783-79185802 MS.gene86922:intron 40.0%
ATCAACCCCACATAGTGAAA+TGG - chr1.2:79186095-79186114 None:intergenic 40.0%
ATCTTCTTCTCCCTAAATGC+TGG - chr1.2:79187306-79187325 None:intergenic 40.0%
CCAAAGCAAACAGTAACGAA+TGG - chr1.2:79186391-79186410 None:intergenic 40.0%
CCATTCGTTACTGTTTGCTT+TGG + chr1.2:79186388-79186407 MS.gene86922:intron 40.0%
CCTTATAAATCAAGGGGCAA+AGG - chr1.2:79186706-79186725 None:intergenic 40.0%
CCTTTGCCCCTTGATTTATA+AGG + chr1.2:79186703-79186722 MS.gene86922:intron 40.0%
CGAGAAAATGGAAATGGTGT+AGG - chr1.2:79186472-79186491 None:intergenic 40.0%
GATGAAAATCCATCCTTCCT+AGG - chr1.2:79187501-79187520 None:intergenic 40.0%
GCAAAGAGATGAGAAATCAC+TGG + chr1.2:79187200-79187219 MS.gene86922:CDS 40.0%
GGTTGAGATGAATGTTTGGA+CGG - chr1.2:79185386-79185405 None:intergenic 40.0%
TAAATACGTTGGGAGGAAAC+TGG + chr1.2:79186348-79186367 MS.gene86922:intron 40.0%
TAATAAGAGCGAGATCTTGG+AGG - chr1.2:79185407-79185426 None:intergenic 40.0%
TACACCTGAAACTTGATGGA+AGG + chr1.2:79185521-79185540 MS.gene86922:intron 40.0%
TAGCTACTACAACATGTGGT+GGG + chr1.2:79186496-79186515 MS.gene86922:intron 40.0%
TATTGTAGCGGCCAAAATAG+AGG + chr1.2:79185795-79185814 MS.gene86922:intron 40.0%
TCAGGTCTATCTAGATGGTT+AGG - chr1.2:79187438-79187457 None:intergenic 40.0%
TCATCAAGAGTAAGGTATGG+GGG + chr1.2:79187516-79187535 MS.gene86922:CDS 40.0%
TCCAGAAAGGAATGAAAGGA+AGG + chr1.2:79187395-79187414 MS.gene86922:CDS 40.0%
TCCTAAGAGCTTAATACCAG+CGG - chr1.2:79185670-79185689 None:intergenic 40.0%
TCCTTCCTTTCATTCCTTTC+TGG - chr1.2:79187399-79187418 None:intergenic 40.0%
TCCTTTCATTCCTTTCTGGA+GGG - chr1.2:79187395-79187414 None:intergenic 40.0%
TCTCACCTTTCAAAGCCATT+TGG + chr1.2:79185976-79185995 MS.gene86922:intron 40.0%
TGCATACACCTGAAACTTGA+TGG + chr1.2:79185517-79185536 MS.gene86922:intron 40.0%
TGGAGGTTGAGATGAATGTT+TGG - chr1.2:79185390-79185409 None:intergenic 40.0%
TGTTTGTGTCAGTACTTCGT+AGG + chr1.2:79185634-79185653 MS.gene86922:intron 40.0%
TTAGCTACTACAACATGTGG+TGG + chr1.2:79186495-79186514 MS.gene86922:intron 40.0%
TTCCTTTCATTCCTTTCTGG+AGG - chr1.2:79187396-79187415 None:intergenic 40.0%
TTGCCCCTTGATTTATAAGG+AGG + chr1.2:79186706-79186725 MS.gene86922:intron 40.0%
TTTACAGCTGTAGATGTGTC+CGG + chr1.2:79185605-79185624 MS.gene86922:intron 40.0%
! AAATCACTGGCTAAACTGGA+TGG + chr1.2:79187213-79187232 MS.gene86922:CDS 40.0%
! AACTTGATGGAAGGCATGTA+TGG + chr1.2:79185530-79185549 MS.gene86922:intron 40.0%
! TAGCTGCCATTTCACTATGT+GGG + chr1.2:79186086-79186105 MS.gene86922:intron 40.0%
! TGAGAAATCACTGGCTAAAC+TGG + chr1.2:79187209-79187228 MS.gene86922:CDS 40.0%
! TTAGCTGCCATTTCACTATG+TGG + chr1.2:79186085-79186104 MS.gene86922:intron 40.0%
!! ACGAAGTACTGACACAAACA+TGG - chr1.2:79185634-79185653 None:intergenic 40.0%
!! TCTTTAAAACCACGGCCAAA+TGG - chr1.2:79185994-79186013 None:intergenic 40.0%
!! TGTGGGGTTGATAACATTGT+TGG + chr1.2:79186103-79186122 MS.gene86922:intron 40.0%
!!! AAACTATTTTTCTGGGGCGT+GGG + chr1.2:79186875-79186894 MS.gene86922:intron 40.0%
!!! GTGTGTGTTTGTTTTATCGG+TGG - chr1.2:79186065-79186084 None:intergenic 40.0%
!!! TCTATTTTGGCCGCTACAAT+AGG - chr1.2:79185796-79185815 None:intergenic 40.0%
AATCCAACAAGCGAGAACCT+AGG + chr1.2:79187481-79187500 MS.gene86922:CDS 45.0%
ACCCTCCAGAAAGGAATGAA+AGG + chr1.2:79187391-79187410 MS.gene86922:CDS 45.0%
ACTGACACAAACATGGACAC+CGG - chr1.2:79185627-79185646 None:intergenic 45.0%
AGAAGCGAATTGCAGAGGAT+AGG + chr1.2:79187457-79187476 MS.gene86922:CDS 45.0%
AGAATGACGACGCTTCGAAT+CGG - chr1.2:79185178-79185197 None:intergenic 45.0%
ATGATCCAACACCTTCTGTG+AGG + chr1.2:79187274-79187293 MS.gene86922:CDS 45.0%
ATTGGTCAGCTTGTACAAGC+TGG + chr1.2:79186318-79186337 MS.gene86922:intron 45.0%
CAACACCTTCTGTGAGGAAA+AGG + chr1.2:79187280-79187299 MS.gene86922:CDS 45.0%
CAAGCTGACCAATTGTATGC+AGG - chr1.2:79186311-79186330 None:intergenic 45.0%
CATGCCTTCCATCAAGTTTC+AGG - chr1.2:79185528-79185547 None:intergenic 45.0%
GAAGCGAATTGCAGAGGATA+GGG + chr1.2:79187458-79187477 MS.gene86922:CDS 45.0%
GAGGAAAAGGCCAGCATTTA+GGG + chr1.2:79187293-79187312 MS.gene86922:CDS 45.0%
GCTTCTCAGGTCTATCTAGA+TGG - chr1.2:79187443-79187462 None:intergenic 45.0%
GGATGATCAGATTGCAAAGC+TGG + chr1.2:79187677-79187696 MS.gene86922:CDS 45.0%
TAGGGAGAAGAAGATCTCAG+TGG + chr1.2:79187311-79187330 MS.gene86922:CDS 45.0%
TCCGCTGGTATTAAGCTCTT+AGG + chr1.2:79185666-79185685 MS.gene86922:intron 45.0%
TGAGATGAATGTTTGGACGG+TGG - chr1.2:79185383-79185402 None:intergenic 45.0%
TGAGGAAAAGGCCAGCATTT+AGG + chr1.2:79187292-79187311 MS.gene86922:CDS 45.0%
TTTGACCTGTCTCCAACCTT+TGG - chr1.2:79187173-79187192 None:intergenic 45.0%
! AGCTGCCATTTCACTATGTG+GGG + chr1.2:79186087-79186106 MS.gene86922:intron 45.0%
! CTAGAAGAGGAAGTGTACCT+TGG - chr1.2:79185216-79185235 None:intergenic 45.0%
! GATCTCAGTGGATTCAAGAG+AGG + chr1.2:79187323-79187342 MS.gene86922:CDS 45.0%
! GCATTGGAAAGCATATACCG+AGG - chr1.2:79186930-79186949 None:intergenic 45.0%
! TGGAAGGCATGTATGGTGTT+TGG + chr1.2:79185537-79185556 MS.gene86922:intron 45.0%
! TTTCATTCCTTTCTGGAGGG+TGG - chr1.2:79187392-79187411 None:intergenic 45.0%
!! CACTTCCTCTTCTAGTTCCA+TGG + chr1.2:79185221-79185240 MS.gene86922:intron 45.0%
!! CTTTTCCTCACAGAAGGTGT+TGG - chr1.2:79187282-79187301 None:intergenic 45.0%
!! GAAGAGACGGTTTTCGAGAA+AGG + chr1.2:79187247-79187266 MS.gene86922:CDS 45.0%
!!! CAAACTATTTTTCTGGGGCG+TGG + chr1.2:79186874-79186893 MS.gene86922:intron 45.0%
!! TATATATCAATATAAAATTA+AGG - chr1.2:79187034-79187053 None:intergenic 5.0%
!!! ATTTAAATAAAATGATTTTA+CGG - chr1.2:79185868-79185887 None:intergenic 5.0%
AAGCGAGAACCTAGGAAGGA+TGG + chr1.2:79187489-79187508 MS.gene86922:CDS 50.0%
ACCTGAGAAGCGAATTGCAG+AGG + chr1.2:79187452-79187471 MS.gene86922:CDS 50.0%
ACTGGTCAAAGGGAAGCTGA+TGG + chr1.2:79187001-79187020 MS.gene86922:CDS 50.0%
AGAGTAAGGTATGGGGGAAG+TGG + chr1.2:79187522-79187541 MS.gene86922:CDS 50.0%
ATACTAGCCACCCTCCAGAA+AGG + chr1.2:79187382-79187401 MS.gene86922:CDS 50.0%
CAACAAGCGAGAACCTAGGA+AGG + chr1.2:79187485-79187504 MS.gene86922:CDS 50.0%
CTAAGCTCGATCGTGAACCA+AGG + chr1.2:79185196-79185215 MS.gene86922:CDS 50.0%
CTTCCTAGGTTCTCGCTTGT+TGG - chr1.2:79187487-79187506 None:intergenic 50.0%
CTTTCAAAGCCATTTGGCCG+TGG + chr1.2:79185982-79186001 MS.gene86922:intron 50.0%
GGAGGCAATAGCAATCACAG+AGG + chr1.2:79187543-79187562 MS.gene86922:CDS 50.0%
GTGAGGCACAATAGCATCTG+CGG - chr1.2:79185824-79185843 None:intergenic 50.0%
TCCTCTGCAATTCGCTTCTC+AGG - chr1.2:79187456-79187475 None:intergenic 50.0%
TGAATGGGGCAATACTGTGC+AGG + chr1.2:79186967-79186986 MS.gene86922:CDS 50.0%
TGCTTTGGCTGTTAACTGCC+TGG + chr1.2:79186403-79186422 MS.gene86922:intron 50.0%
TTTACTGTCACTGTCGCAGC+TGG - chr1.2:79187354-79187373 None:intergenic 50.0%
! ATCTGCGGCAGCCTCTATTT+TGG - chr1.2:79185809-79185828 None:intergenic 50.0%
! CTACTACAACATGTGGTGGG+TGG + chr1.2:79186499-79186518 MS.gene86922:intron 50.0%
! GCATATACCGAGGCACTTGA+AGG - chr1.2:79186920-79186939 None:intergenic 50.0%
! TTTCTGGAGGGTGGCTAGTA+TGG - chr1.2:79187383-79187402 None:intergenic 50.0%
!! CACGGCCAAATGGCTTTGAA+AGG - chr1.2:79185984-79186003 None:intergenic 50.0%
!! CGTAGGTGTTGTTTATCCGC+TGG + chr1.2:79185651-79185670 MS.gene86922:intron 50.0%
!! TGGTCGAAGCACTGGTCAAA+GGG + chr1.2:79186991-79187010 MS.gene86922:CDS 50.0%
!! TTGGTCGAAGCACTGGTCAA+AGG + chr1.2:79186990-79187009 MS.gene86922:CDS 50.0%
ATGACGACGCTTCGAATCGG+AGG - chr1.2:79185175-79185194 None:intergenic 55.0%
GTAAGGTATGGGGGAAGTGG+AGG + chr1.2:79187525-79187544 MS.gene86922:CDS 55.0%
TGCATGTCGCGTTGCAGTCA+GGG - chr1.2:79185761-79185780 None:intergenic 55.0%
TGGGGCAATACTGTGCAGGT+TGG + chr1.2:79186971-79186990 MS.gene86922:CDS 55.0%
TTGCATGTCGCGTTGCAGTC+AGG - chr1.2:79185762-79185781 None:intergenic 55.0%
! ACCGAGGCACTTGAAGGCAT+GGG - chr1.2:79186914-79186933 None:intergenic 55.0%
! TACCGAGGCACTTGAAGGCA+TGG - chr1.2:79186915-79186934 None:intergenic 55.0%
!!! GCTGGCCTTTTCCTCACAGA+AGG - chr1.2:79187288-79187307 None:intergenic 55.0%
CCCCATGCCTTCAAGTGCCT+CGG + chr1.2:79186910-79186929 MS.gene86922:intron 60.0%
GCATGTCGCGTTGCAGTCAG+GGG - chr1.2:79185760-79185779 None:intergenic 60.0%
! CCGAGGCACTTGAAGGCATG+GGG - chr1.2:79186913-79186932 None:intergenic 60.0%
!! GTGCAGGTTGGTCGAAGCAC+TGG + chr1.2:79186983-79187002 MS.gene86922:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 79185156 79187719 79185156 ID=MS.gene86922
chr1.2 mRNA 79185156 79187719 79185156 ID=MS.gene86922.t1;Parent=MS.gene86922
chr1.2 exon 79185156 79185217 79185156 ID=MS.gene86922.t1.exon1;Parent=MS.gene86922.t1
chr1.2 CDS 79185156 79185217 79185156 ID=cds.MS.gene86922.t1;Parent=MS.gene86922.t1
chr1.2 exon 79185301 79185430 79185301 ID=MS.gene86922.t1.exon2;Parent=MS.gene86922.t1
chr1.2 CDS 79185301 79185430 79185301 ID=cds.MS.gene86922.t1;Parent=MS.gene86922.t1
chr1.2 exon 79186962 79187022 79186962 ID=MS.gene86922.t1.exon3;Parent=MS.gene86922.t1
chr1.2 CDS 79186962 79187022 79186962 ID=cds.MS.gene86922.t1;Parent=MS.gene86922.t1
chr1.2 exon 79187139 79187719 79187139 ID=MS.gene86922.t1.exon4;Parent=MS.gene86922.t1
chr1.2 CDS 79187139 79187719 79187139 ID=cds.MS.gene86922.t1;Parent=MS.gene86922.t1
Gene Sequence

>MS.gene86922

ATGTCTCATCGAGATTCCTCCGATTCGAAGCGTCGTCATTCTAAGCTCGATCGTGAACCAAGGTACACTTCCTCTTCTAGTTCCATGGATTTAGATGATTGATTATTTGGTTTTTGAATGTTGATTATGGTGATGTTTGTTTTAGCCCTAAGAAATATAGAAGAGATGGAAAACAAGATAGATATAGAGAAAGAGAAAGATTAACCACAAATGATCATTCTGAACCACCGTCCAAACATTCATCTCAACCTCCAAGATCTCGCTCTTATTATCAGGTCATCTTTATTTTACTCTTGATTGCTTCAGCTTAATGTGCTTCTTTATATTTGGACCAACTTCTGTTCCTAACTACATTCAAATTTGCATACACCTGAAACTTGATGGAAGGCATGTATGGTGTTTGGCACTGAGATATTTGATTGCATTCAATTAATTCATTTTTTCAAGTTTTTACAGCTGTAGATGTGTCCGGTGTCCATGTTTGTGTCAGTACTTCGTAGGTGTTGTTTATCCGCTGGTATTAAGCTCTTAGGAAAGTGCACCAAACCAAACTTAAAATGTTGGAAAACAATGTTGTCAATAGCATATTATAGCGGAGCGACCCCTGACTGCAACGCGACATGCAATAGTAGAGCAACCTATTGTAGCGGCCAAAATAGAGGCTGCCGCAGATGCTATTGTGCCTCACCTCAGAATGAGATAAAAAATTCCGTAAAATCATTTTATTTAAATGTTAGGGCTTTTCATTTAATTTCAGATCTCACTCCATTTAGGGAGACATCCATTTATGCAATGTTTGGACATGTGGTAGACTTGCTTTTCTCACCTTTCAAAGCCATTTGGCCGTGGTTTTAAAGAAGCTACATATTGTAGCATTAAACGAATGTGCGTTGCCGTTTGTTTATTCCACCGATAAAACAAACACACACTTAGCTGCCATTTCACTATGTGGGGTTGATAACATTGTTGGAAAAGGAAAAAAAATATCTTTAGTCTCCTTTGTTTAAAAATAAATGATTTCTCTATTCACAAAGAGGAGAGTTGCATGTGAATTAGCATTAGCATTGCACAAAAACATAACTCAATCATTATTAAACCTTTAGCTCTAAATCAAGTAGTTAACTTGGGTTCACAGTTATTGTTTTGTATATCCCTGCATACAATTGGTCAGCTTGTACAAGCTGGTAAACAATAAATACGTTGGGAGGAAACTGGGAGAAGAACATTGTACACCATTCGTTACTGTTTGCTTTGGCTGTTAACTGCCTGGTACAAGTTGTGAGCAATTTTATAACCTTAATCATAATGCAAATCCTACACCATTTCCATTTTCTCGATGTTAGCTACTACAACATGTGGTGGGTGGAAAGTTACTTATTCGAGATTACCAGGATTGCCGTATATATTGTTGTATTGGGTGAAATACAAACCATAAATGTTAATAAAAACAACTGGAATTTGCTTTGACGGTAAAATATTGTATGAATAATCTATACAGATGACAAAGCTCAAATCCTTCACCATAAATGTTGAGATTGGTTTTTATTCCTTTGCCCCTTGATTTATAAGGAGGTCAGTGTTTATCGTCAAATACCTTTATATAAACAGTATTGGAAAGAAAACATCTTAATGGACAATGAAGATAACTTGGAAAATTCATTTTTTAATTACAGATATAAAATCCCTTTTTATATGCTATAGATGTTAATCAGTGTATGCAAACTATTTTTCTGGGGCGTGGGATTCTTATTCAGCCCCATGCCTTCAAGTGCCTCGGTATATGCTTTCCAATGCATGTTTTAACAGCATCATGAATGGGGCAATACTGTGCAGGTTGGTCGAAGCACTGGTCAAAGGGAAGCTGATGGTGAGTTTCCTTAATTTTATATTGATATATATTATTTTCTTTTGGCTTTATTGTATATGGATGGTTTGGAGATTTACTCGATATACTGAAGCCCAAAATTCTTCGATTCCAAACAGAAAAGGTATATGCTCAAAGTAAGGAGAATAACCAAAGGTTGGAGACAGGTCAAAGTAGAGAGCAAAGAGATGAGAAATCACTGGCTAAACTGGATGGTAATTTTCAGAGAAGAGACGGTTTTCGAGAAAGGAAAGATGATCCAACACCTTCTGTGAGGAAAAGGCCAGCATTTAGGGAGAAGAAGATCTCAGTGGATTCAAGAGAGGCTAATCCAGCTGCGACAGTGACAGTAAAGTCAAGCCATACTAGCCACCCTCCAGAAAGGAATGAAAGGAAGGAGGAAAGAAGCAGCAATCCTAACCATCTAGATAGACCTGAGAAGCGAATTGCAGAGGATAGGGAATCCAACAAGCGAGAACCTAGGAAGGATGGATTTTCATCAAGAGTAAGGTATGGGGGAAGTGGAGGCAATAGCAATCACAGAGGAAGAGACAAATTTAATGGAAGACAAGATAATCATCCTATTAAGACTCAAACAGAGAAGTGGAAACATGATTTGTATCAAGAGGTTAACAAAGATTCTATTCCAAAGAATGAGGATGATCAGATTGCAAAGCTGGAAGCACTCTTAGCTTCATAA
Protein sequence

>MS.gene86922.t1

MSHRDSSDSKRRHSKLDREPSPKKYRRDGKQDRYRERERLTTNDHSEPPSKHSSQPPRSRSYYQHHEWGNTVQVGRSTGQREADEKVYAQSKENNQRLETGQSREQRDEKSLAKLDGNFQRRDGFRERKDDPTPSVRKRPAFREKKISVDSREANPAATVTVKSSHTSHPPERNERKEERSSNPNHLDRPEKRIAEDRESNKREPRKDGFSSRVRYGGSGGNSNHRGRDKFNGRQDNHPIKTQTEKWKHDLYQEVNKDSIPKNEDDQIAKLEALLAS