AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86993

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86993.t1 MTR_2g104940 98.387 310 5 0 1 310 1 310 0.0 619
MS.gene86993.t1 MTR_4g006730 83.548 310 43 3 4 310 1 305 0.0 506
MS.gene86993.t1 MTR_4g006730 85.124 242 28 3 4 242 1 237 7.06e-142 401
MS.gene86993.t1 MTR_4g006730 87.156 218 28 0 93 310 1 218 6.16e-132 374
MS.gene86993.t1 MTR_4g006630 86.697 218 29 0 93 310 1 218 2.95e-131 372
MS.gene86993.t1 MTR_1g097840 58.224 304 107 4 11 310 13 300 4.38e-110 322
MS.gene86993.t1 MTR_1g097840 58.475 236 82 2 11 242 13 236 4.90e-82 249
MS.gene86993.t1 MTR_8g087710 48.473 262 130 2 45 303 4 263 1.50e-76 235
MS.gene86993.t1 MTR_8g087720 51.542 227 105 2 80 303 38 262 1.41e-74 230
MS.gene86993.t1 MTR_2g017660 51.046 239 113 2 76 310 35 273 2.23e-74 230
MS.gene86993.t1 MTR_2g017590 49.206 252 119 3 56 303 14 260 7.95e-73 226
MS.gene86993.t1 MTR_2g017620 49.206 252 119 3 56 303 2 248 2.97e-72 224
MS.gene86993.t1 MTR_2g017620 49.206 252 119 3 56 303 16 262 3.15e-72 224
MS.gene86993.t1 MTR_4g006650 82.979 141 15 1 81 221 5 136 4.23e-72 226
MS.gene86993.t1 MTR_7g010650 46.121 232 122 1 75 306 32 260 1.92e-71 222
MS.gene86993.t1 MTR_4g084880 44.697 264 134 4 56 310 8 268 2.22e-70 219
MS.gene86993.t1 MTR_2g017570 51.584 221 102 2 76 293 34 252 2.16e-69 217
MS.gene86993.t1 MTR_2g017610 52.535 217 98 2 76 289 34 248 2.80e-69 217
MS.gene86993.t1 MTR_2g017620 54.348 184 77 2 124 303 1 181 2.29e-60 191
MS.gene86993.t1 MTR_5g079570 45.495 222 116 3 76 293 36 256 2.54e-57 186
MS.gene86993.t1 MTR_3g008920 47.685 216 109 3 76 289 22 235 3.03e-55 180
MS.gene86993.t1 MTR_5g063930 43.779 217 116 2 80 292 37 251 5.29e-55 180
MS.gene86993.t1 MTR_8g087720 53.012 166 73 2 80 242 38 201 1.80e-50 166
MS.gene86993.t1 MTR_1g070385 40.175 229 137 0 78 306 60 288 4.43e-48 172
MS.gene86993.t1 MTR_8g087720 50.685 146 67 2 80 222 38 181 6.01e-39 136
MS.gene86993.t1 MTR_7g010650 43.243 148 84 0 75 222 32 179 1.80e-38 135
MS.gene86993.t1 MTR_2g101370 39.286 224 116 8 84 293 24 241 3.18e-32 120
MS.gene86993.t1 MTR_1g006490 37.073 205 109 7 85 277 24 220 6.17e-29 111
MS.gene86993.t1 MTR_8g013680 35.749 207 115 4 84 277 23 224 4.00e-27 106
MS.gene86993.t1 MTR_1g077540 34.404 218 135 3 84 293 25 242 5.51e-26 103
MS.gene86993.t1 MTR_1g006590 36.098 205 111 8 85 277 24 220 6.88e-25 100
MS.gene86993.t1 MTR_6g069040 32.287 223 143 3 84 298 25 247 2.59e-24 99.4
MS.gene86993.t1 MTR_2g094270 31.120 241 132 7 71 278 22 261 2.40e-23 97.4
MS.gene86993.t1 MTR_5g012810 34.783 207 116 4 84 277 23 223 5.03e-23 95.9
MS.gene86993.t1 MTR_3g118010 30.453 243 142 7 73 288 19 261 8.66e-23 95.9
MS.gene86993.t1 MTR_4g063090 33.168 202 127 4 85 278 26 227 1.65e-21 91.7
MS.gene86993.t1 MTR_1g115405 29.075 227 153 3 84 302 25 251 7.33e-21 90.1
MS.gene86993.t1 MTR_2g094270 30.973 226 125 6 71 266 22 246 1.83e-20 89.4
MS.gene86993.t1 MTR_7g101190 28.452 239 140 7 71 278 21 259 1.42e-18 84.3
MS.gene86993.t1 MTR_7g103030 33.010 206 121 6 84 277 25 225 1.91e-18 83.2
MS.gene86993.t1 MTR_3g070210 29.508 244 144 8 73 288 37 280 3.68e-18 83.2
MS.gene86993.t1 MTR_8g098375 29.487 234 137 8 73 278 34 267 1.29e-17 81.6
MS.gene86993.t1 MTR_4g415300 29.717 212 131 7 84 278 60 270 1.80e-16 78.6
MS.gene86993.t1 MTR_5g010150 30.189 212 131 6 84 278 56 267 2.62e-16 78.2
MS.gene86993.t1 MTR_4g059390 27.197 239 141 7 73 278 21 259 3.05e-16 77.8
MS.gene86993.t1 MTR_3g070210 30.093 216 126 7 73 263 37 252 1.21e-15 75.9
MS.gene86993.t1 MTR_7g023350 27.753 227 156 4 84 302 25 251 1.27e-15 75.5
MS.gene86993.t1 MTR_5g082070 28.692 237 135 8 73 278 37 270 3.56e-15 74.7
MS.gene86993.t1 MTR_2g060360 28.571 224 145 6 84 296 23 242 9.48e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86993.t1 AT1G80760 77.564 312 58 4 4 310 1 305 6.48e-167 466
MS.gene86993.t1 AT4G10380 67.416 267 75 3 46 310 48 304 4.94e-119 344
MS.gene86993.t1 AT5G37820 46.484 256 129 4 52 303 16 267 7.96e-72 224
MS.gene86993.t1 AT5G37820 46.484 256 129 4 52 303 52 303 1.20e-71 224
MS.gene86993.t1 AT4G19030 43.686 293 151 5 29 309 2 292 1.51e-70 221
MS.gene86993.t1 AT5G37810 46.964 247 125 3 59 303 25 267 5.68e-70 219
MS.gene86993.t1 AT4G18910 41.472 299 146 4 29 309 2 289 2.78e-69 218
MS.gene86993.t1 AT1G31885 45.600 250 128 2 68 309 31 280 2.52e-66 211
MS.gene86993.t1 AT1G31885 46.903 226 112 2 92 309 1 226 2.85e-61 196
MS.gene86993.t1 AT2G34390 40.217 276 148 5 46 310 13 282 1.79e-54 179
MS.gene86993.t1 AT2G34390 42.083 240 123 4 81 310 7 240 1.49e-51 170
MS.gene86993.t1 AT4G19030 44.000 225 112 5 29 241 2 224 2.54e-49 164
MS.gene86993.t1 AT4G18910 42.241 232 105 4 29 242 2 222 1.99e-48 161
MS.gene86993.t1 AT3G06100 38.208 212 130 1 80 290 46 257 1.45e-43 150
MS.gene86993.t1 AT3G06100 38.389 211 129 1 84 293 2 212 5.97e-43 147
MS.gene86993.t1 AT4G19030 41.748 206 106 5 29 222 2 205 1.49e-38 136
MS.gene86993.t1 AT2G34390 38.942 208 113 4 46 242 13 217 9.03e-35 126
MS.gene86993.t1 AT3G16240 37.198 207 112 5 84 277 23 224 1.14e-29 113
MS.gene86993.t1 AT2G34390 37.368 190 105 4 46 224 13 199 8.63e-28 108
MS.gene86993.t1 AT2G25810 35.885 209 116 7 80 277 19 220 3.33e-26 104
MS.gene86993.t1 AT5G47450 34.783 230 124 7 84 292 23 247 3.69e-26 104
MS.gene86993.t1 AT2G29870 46.957 115 58 1 196 310 22 133 1.10e-25 100
MS.gene86993.t1 AT4G17340 33.816 207 119 4 84 277 23 224 2.58e-24 99.4
MS.gene86993.t1 AT3G26520 34.783 207 117 6 84 277 26 227 7.81e-23 95.5
MS.gene86993.t1 AT2G36830 34.783 207 117 6 84 277 25 226 8.49e-23 95.5
MS.gene86993.t1 AT4G35100 30.864 243 141 7 73 288 20 262 4.05e-22 94.0
MS.gene86993.t1 AT4G35100 30.864 243 141 7 73 288 20 262 4.05e-22 94.0
MS.gene86993.t1 AT2G16850 30.328 244 141 7 73 288 18 260 4.65e-22 94.0
MS.gene86993.t1 AT5G60660 28.689 244 141 7 71 282 21 263 1.70e-20 89.7
MS.gene86993.t1 AT3G06100 34.532 139 90 1 156 293 41 179 7.65e-20 85.9
MS.gene86993.t1 AT3G54820 30.833 240 133 7 71 278 20 258 1.90e-19 87.0
MS.gene86993.t1 AT1G73190 30.493 223 142 4 84 293 28 250 1.41e-18 84.0
MS.gene86993.t1 AT4G01470 30.275 218 134 5 84 288 25 237 2.29e-18 83.2
MS.gene86993.t1 AT1G17810 29.956 227 142 6 84 295 28 252 8.73e-18 82.0
MS.gene86993.t1 AT2G37170 27.308 260 145 8 51 278 10 257 1.34e-17 81.6
MS.gene86993.t1 AT3G53420 27.917 240 140 7 71 278 21 259 1.60e-17 81.6
MS.gene86993.t1 AT3G53420 27.917 240 140 7 71 278 21 259 1.60e-17 81.6
MS.gene86993.t1 AT2G37170 27.308 260 145 8 51 278 53 300 2.97e-17 81.3
MS.gene86993.t1 AT2G45960 31.915 235 129 9 73 278 34 266 8.06e-17 79.3
MS.gene86993.t1 AT2G37180 26.923 260 146 8 51 278 10 257 1.08e-16 79.0
MS.gene86993.t1 AT4G23400 30.189 212 130 6 84 278 57 267 1.31e-16 79.0
MS.gene86993.t1 AT4G00430 29.487 234 136 8 73 278 35 267 1.43e-16 79.0
MS.gene86993.t1 AT1G31885 41.758 91 53 0 66 156 29 119 2.03e-16 75.1
MS.gene86993.t1 AT1G01620 30.660 212 129 6 84 278 56 266 2.41e-16 78.2
MS.gene86993.t1 AT2G39010 28.333 240 137 7 73 278 20 258 2.52e-16 78.2
MS.gene86993.t1 AT3G61430 31.455 213 126 7 84 278 56 266 3.33e-16 77.8
MS.gene86993.t1 AT3G61430 31.455 213 126 7 84 278 56 266 3.33e-16 77.8
MS.gene86993.t1 AT3G47440 27.586 232 151 5 84 303 27 253 2.32e-15 74.7
MS.gene86993.t1 AT1G01620 32.402 179 106 5 114 278 17 194 1.39e-14 72.0
MS.gene86993.t1 AT2G45960 30.594 219 126 7 73 266 34 251 2.07e-14 72.8
MS.gene86993.t1 AT2G45960 31.818 220 122 8 73 266 34 251 2.55e-14 72.0

Find 90 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTGACGCTCCTGTTAT+TGG 0.213047 2.3:-164025 None:intergenic
CATCTTCTAAGTTCCATTCT+TGG 0.216409 2.3:-162844 None:intergenic
GTGACTCCACCACTCATAAA+AGG 0.246044 2.3:-163530 None:intergenic
TCTCCGAGCCTTGTGTCTTT+TGG 0.294166 2.3:-163146 None:intergenic
CTTTGCATTATCATCTTCTT+CGG 0.298809 2.3:-164170 None:intergenic
CAGATAGGAGCCGAGTTTAT+AGG 0.306620 2.3:+163078 MS.gene86993:intron
CCAGGAGTGCCTGGTGTTGC+TGG 0.322227 2.3:-162725 None:intergenic
CCTGGTGTTGCTGGTGTTGA+TGG 0.322832 2.3:-162716 None:intergenic
GTATATTGGTGCACAAGTTT+TGG 0.339591 2.3:+163466 MS.gene86993:CDS
AGATAGGAGCCGAGTTTATA+GGG 0.358525 2.3:+163079 MS.gene86993:intron
GCTGGACCTAGAGTTCTTAC+TGG 0.377381 2.3:-164049 None:intergenic
CTGCGTTGCAGGCCAATAAC+AGG 0.388557 2.3:+164013 MS.gene86993:intron
CTCTCCACCGGCCACATCTC+TGG 0.392505 2.3:+163219 MS.gene86993:CDS
ACCGTTCCTTCCGGAGGATA+TGG 0.393092 2.3:+163551 MS.gene86993:CDS
TAGTTATTTGCAGCTATAGC+TGG 0.401428 2.3:-164067 None:intergenic
ATTGGGTGTGCTACCTCTAC+TGG 0.401500 2.3:+163174 MS.gene86993:CDS
CAAATAACTACAAAGCCATA+TGG 0.404031 2.3:+164080 MS.gene86993:CDS
ACATTGATTCTGATGTTTGC+TGG 0.409739 2.3:+163102 MS.gene86993:CDS
CAAGGCTCGGAGACTCTGAT+TGG 0.413496 2.3:+163156 MS.gene86993:CDS
TTTGCATTATCATCTTCTTC+GGG 0.414167 2.3:-164169 None:intergenic
TGCAAGAGGCACAGGAGGAG+GGG 0.422658 2.3:-162903 None:intergenic
CATTGATTCTGATGTTTGCT+GGG 0.434089 2.3:+163103 MS.gene86993:CDS
CTCAAGAATTGTAAATGCTT+CGG 0.434143 2.3:+162818 MS.gene86993:CDS
CCACCTAGAGCTCCAAGTAT+TGG 0.435294 2.3:-164118 None:intergenic
GGTGGAGTCACCGTTCCTTC+CGG 0.437337 2.3:+163542 MS.gene86993:CDS
GTGCTGCATTTGCTCTCAAA+GGG 0.443470 2.3:+163498 MS.gene86993:CDS
TTTGCAAGAGGCACAGGAGG+AGG 0.446500 2.3:-162905 None:intergenic
TATCTCTTAGCTCCAATACT+TGG 0.455159 2.3:+164106 MS.gene86993:CDS
AGTTGATCATCTTCTAAAGC+TGG 0.459544 2.3:-164215 None:intergenic
TTGCAGGTGCCTATGTATAT+TGG 0.461576 2.3:+163452 MS.gene86993:intron
CTTCAATGCAGCAAAAGAAA+TGG 0.468532 2.3:-163265 None:intergenic
ATGCTCAATATACTAATAGC+AGG 0.472157 2.3:+163894 MS.gene86993:CDS
TTGCAAGAGGCACAGGAGGA+GGG 0.478192 2.3:-162904 None:intergenic
AGAGGTACACCAGGAGTGCC+TGG 0.482854 2.3:-162734 None:intergenic
AAATAACTACAAAGCCATAT+GGG 0.491686 2.3:+164081 MS.gene86993:CDS
AATGCTTCGGTGTCCAAGAA+TGG 0.493128 2.3:+162831 MS.gene86993:CDS
CTTGGAGCTCTAGGTGGGGC+AGG 0.496173 2.3:+164124 MS.gene86993:CDS
GCAAGAGGCACAGGAGGAGG+GGG 0.497170 2.3:-162902 None:intergenic
GCATTTACAATTCTTGAGTA+GGG 0.497185 2.3:-162813 None:intergenic
GCAAAAGAAATGGTGACTGC+AGG 0.502542 2.3:-163255 None:intergenic
CAAGAATGGAACTTAGAAGA+TGG 0.514046 2.3:+162845 MS.gene86993:CDS
CCAGCAACACCAGGCACTCC+TGG 0.515961 2.3:+162725 MS.gene86993:CDS
GTTGATCATCTTCTAAAGCT+GGG 0.517734 2.3:-164214 None:intergenic
AATGGAACATATTAATAATG+AGG 0.519868 2.3:+162688 None:intergenic
ATGAACCCAGTAAGAACTCT+AGG 0.522426 2.3:+164043 MS.gene86993:CDS
TAATCTAATGTTCGTTGTCA+CGG 0.524885 2.3:+163607 MS.gene86993:CDS
GCTCCAATACTTGGAGCTCT+AGG 0.532633 2.3:+164115 MS.gene86993:CDS
AGCATTTACAATTCTTGAGT+AGG 0.536216 2.3:-162814 None:intergenic
TGACTCCACCACTCATAAAA+GGG 0.537986 2.3:-163529 None:intergenic
GCTTTGGTTACATGCTTCCA+CGG 0.548483 2.3:-163294 None:intergenic
TGTGCTGCATTTGCTCTCAA+AGG 0.550088 2.3:+163497 MS.gene86993:CDS
AAGGCTCGGAGACTCTGATT+GGG 0.552417 2.3:+163157 MS.gene86993:CDS
GGTTTAAGTCTGAGAGAAGC+GGG 0.553612 2.3:+162765 MS.gene86993:CDS
AATGTTCGTTGTCACGGCAG+TGG 0.554743 2.3:+163613 MS.gene86993:CDS
AAGTCTGAGAGAAGCGGGAA+TGG 0.555069 2.3:+162770 MS.gene86993:CDS
CAGAGATGTGGCCGGTGGAG+AGG 0.557528 2.3:-163218 None:intergenic
CTGGACCTAGAGTTCTTACT+GGG 0.557635 2.3:-164048 None:intergenic
TGCTACCTCTACTGGTCTTG+CGG 0.560160 2.3:+163182 MS.gene86993:CDS
CAATACTTGGAGCTCTAGGT+GGG 0.562103 2.3:+164119 MS.gene86993:CDS
GAACTAGCAGGAATTGCAGT+GGG 0.569469 2.3:+163861 MS.gene86993:CDS
CCAATACTTGGAGCTCTAGG+TGG 0.572700 2.3:+164118 MS.gene86993:CDS
ACCATATCCTCCGGAAGGAA+CGG 0.576185 2.3:-163552 None:intergenic
AGCTAAGAGATAGACCCATA+TGG 0.576739 2.3:-164095 None:intergenic
CCATCAACACCAGCAACACC+AGG 0.577155 2.3:+162716 MS.gene86993:CDS
CTGCATTGAAGCACTTCCCG+TGG 0.578067 2.3:+163277 MS.gene86993:CDS
CATAACCGCAAGACCAGTAG+AGG 0.586587 2.3:-163187 None:intergenic
CATTAATGAGCAGGTAACTG+TGG 0.587885 2.3:-162876 None:intergenic
ATGATCATCATCCTCTCCAC+CGG 0.588983 2.3:+163207 MS.gene86993:CDS
CAAAAGAAATGGTGACTGCA+GGG 0.592708 2.3:-163254 None:intergenic
AAAAGCTTGACCATATCCTC+CGG 0.599263 2.3:-163561 None:intergenic
ACTTGTGCACCAATATACAT+AGG 0.600697 2.3:-163461 None:intergenic
AATCAATGTCCCTATAAACT+CGG 0.603006 2.3:-163088 None:intergenic
CTTTGGTTACATGCTTCCAC+GGG 0.610743 2.3:-163293 None:intergenic
AGCACCAGAGATGTGGCCGG+TGG 0.615438 2.3:-163223 None:intergenic
TGAGATGAGCACCAGAGATG+TGG 0.617163 2.3:-163230 None:intergenic
TGCTGCATTTGCTCTCAAAG+GGG 0.620449 2.3:+163499 MS.gene86993:CDS
GGAGTCACCGTTCCTTCCGG+AGG 0.622617 2.3:+163545 MS.gene86993:CDS
ACTCACAGCTCTTGTGTCGG+TGG 0.623869 2.3:-163636 None:intergenic
GGGTTTAAGTCTGAGAGAAG+CGG 0.625558 2.3:+162764 MS.gene86993:CDS
ATGTAGGTAGGAGAACTAGC+AGG 0.625936 2.3:+163849 MS.gene86993:intron
ATTAATGAGCAGGTAACTGT+GGG 0.626119 2.3:-162875 None:intergenic
AGAACTAGCAGGAATTGCAG+TGG 0.628860 2.3:+163860 MS.gene86993:CDS
TTGATCATCTTCTAAAGCTG+GGG 0.642052 2.3:-164213 None:intergenic
GAACCAAAAGACACAAGGCT+CGG 0.650309 2.3:+163143 MS.gene86993:CDS
GCTTGACCATATCCTCCGGA+AGG 0.651188 2.3:-163557 None:intergenic
AATACTTGGAGCTCTAGGTG+GGG 0.656773 2.3:+164120 MS.gene86993:CDS
TGCTCAATATACTAATAGCA+GGG 0.657754 2.3:+163895 MS.gene86993:CDS
AATACTCACAGCTCTTGTGT+CGG 0.671145 2.3:-163639 None:intergenic
ATGAGCACCAGAGATGTGGC+CGG 0.675762 2.3:-163226 None:intergenic
ATTGTGAACCAAAAGACACA+AGG 0.703696 2.3:+163138 MS.gene86993:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTATAAATTATAATTGC+AGG + chr2.3:163436-163455 MS.gene86993:intron 15.0%
!! TAATTTCAACAACTTTAATT+AGG - chr2.3:163346-163365 None:intergenic 15.0%
!!! CATATGTTCAAATTTTAATT+TGG - chr2.3:163764-163783 None:intergenic 15.0%
!! ACAACTTTAATTAGGAAATT+AGG - chr2.3:163338-163357 None:intergenic 20.0%
!! ATAACCATAAACATCAATTA+GGG - chr2.3:163831-163850 None:intergenic 20.0%
!! TATTAGCTAAAAGTTACTAT+AGG - chr2.3:163669-163688 None:intergenic 20.0%
!! TTAAAATTTGAACATATGCT+TGG + chr2.3:163766-163785 MS.gene86993:intron 20.0%
! AAATAACTACAAAGCCATAT+GGG + chr2.3:164081-164100 MS.gene86993:CDS 25.0%
! AGTTTGAAAAGAAAAAGAGA+AGG + chr2.3:163707-163726 MS.gene86993:intron 25.0%
! ATATATATGTTCATGACAAG+TGG - chr2.3:163984-164003 None:intergenic 25.0%
! ATTGATGTTTATGGTTATGT+AGG + chr2.3:163833-163852 MS.gene86993:intron 25.0%
! ATTTATGTGTGTCTAATCTT+TGG + chr2.3:162978-162997 MS.gene86993:intron 25.0%
! CATAACCATAAACATCAATT+AGG - chr2.3:163832-163851 None:intergenic 25.0%
! TATATATGTTCATGACAAGT+GGG - chr2.3:163983-164002 None:intergenic 25.0%
! TGTCATGAACATATATATGA+TGG + chr2.3:163986-164005 MS.gene86993:intron 25.0%
!!! ATATATACCTTTTTTGCAAG+AGG - chr2.3:162920-162939 None:intergenic 25.0%
AATCAATGTCCCTATAAACT+CGG - chr2.3:163091-163110 None:intergenic 30.0%
AGCATTTACAATTCTTGAGT+AGG - chr2.3:162817-162836 None:intergenic 30.0%
ATATATGTTCATGACAAGTG+GGG - chr2.3:163982-164001 None:intergenic 30.0%
ATGCTCAATATACTAATAGC+AGG + chr2.3:163894-163913 MS.gene86993:CDS 30.0%
ATGTTTATGGTTATGTAGGT+AGG + chr2.3:163837-163856 MS.gene86993:intron 30.0%
CAAATAACTACAAAGCCATA+TGG + chr2.3:164080-164099 MS.gene86993:CDS 30.0%
CTCAAGAATTGTAAATGCTT+CGG + chr2.3:162818-162837 MS.gene86993:CDS 30.0%
CTTTGCATTATCATCTTCTT+CGG - chr2.3:164173-164192 None:intergenic 30.0%
GCATTTACAATTCTTGAGTA+GGG - chr2.3:162816-162835 None:intergenic 30.0%
TAATCTAATGTTCGTTGTCA+CGG + chr2.3:163607-163626 MS.gene86993:CDS 30.0%
TAATTAGGAAATTAGGCTGA+AGG - chr2.3:163331-163350 None:intergenic 30.0%
TACTTAGCACTTCAATGATA+TGG - chr2.3:163024-163043 None:intergenic 30.0%
TGCTCAATATACTAATAGCA+GGG + chr2.3:163895-163914 MS.gene86993:CDS 30.0%
TTTGCATTATCATCTTCTTC+GGG - chr2.3:164172-164191 None:intergenic 30.0%
AAACTACCTGTGTTACAGAT+AGG + chr2.3:163063-163082 MS.gene86993:intron 35.0%
ACTTGTGCACCAATATACAT+AGG - chr2.3:163464-163483 None:intergenic 35.0%
ATTAATGAGCAGGTAACTGT+GGG - chr2.3:162878-162897 None:intergenic 35.0%
ATTGTGAACCAAAAGACACA+AGG + chr2.3:163138-163157 MS.gene86993:CDS 35.0%
CAAGAATGGAACTTAGAAGA+TGG + chr2.3:162845-162864 MS.gene86993:CDS 35.0%
CATCTTCTAAGTTCCATTCT+TGG - chr2.3:162847-162866 None:intergenic 35.0%
CTTCAATGCAGCAAAAGAAA+TGG - chr2.3:163268-163287 None:intergenic 35.0%
GAAATTAGGCTGAAGGATTA+AGG - chr2.3:163324-163343 None:intergenic 35.0%
GAAGGATTAAGGAAAAGCTT+TGG - chr2.3:163313-163332 None:intergenic 35.0%
GAGTCCCTAATTGATGTTTA+TGG + chr2.3:163824-163843 MS.gene86993:intron 35.0%
GATGATATTATCCCCTATGT+CGG - chr2.3:163742-163761 None:intergenic 35.0%
TAGTTATTTGCAGCTATAGC+TGG - chr2.3:164070-164089 None:intergenic 35.0%
TATCTCTTAGCTCCAATACT+TGG + chr2.3:164106-164125 MS.gene86993:CDS 35.0%
! ATCTGTAACACAGGTAGTTT+TGG - chr2.3:163063-163082 None:intergenic 35.0%
! GTATATTGGTGCACAAGTTT+TGG + chr2.3:163466-163485 MS.gene86993:CDS 35.0%
! GTTTTTCACCCTTTTATGAG+TGG + chr2.3:163521-163540 MS.gene86993:CDS 35.0%
!! ACATTGATTCTGATGTTTGC+TGG + chr2.3:163102-163121 MS.gene86993:CDS 35.0%
!! CATTGATTCTGATGTTTGCT+GGG + chr2.3:163103-163122 MS.gene86993:CDS 35.0%
AAAAGCTTGACCATATCCTC+CGG - chr2.3:163564-163583 None:intergenic 40.0%
AATACTCACAGCTCTTGTGT+CGG - chr2.3:163642-163661 None:intergenic 40.0%
AGATAGGAGCCGAGTTTATA+GGG + chr2.3:163079-163098 MS.gene86993:intron 40.0%
AGCTAAGAGATAGACCCATA+TGG - chr2.3:164098-164117 None:intergenic 40.0%
ATGAACCCAGTAAGAACTCT+AGG + chr2.3:164043-164062 MS.gene86993:CDS 40.0%
CAAAAGAAATGGTGACTGCA+GGG - chr2.3:163257-163276 None:intergenic 40.0%
CATTAATGAGCAGGTAACTG+TGG - chr2.3:162879-162898 None:intergenic 40.0%
GACTTAAACCCACCAAAAAG+AGG - chr2.3:162755-162774 None:intergenic 40.0%
GAGAGTGATAAGTGAGAGTT+AGG - chr2.3:163932-163951 None:intergenic 40.0%
TGACTCCACCACTCATAAAA+GGG - chr2.3:163532-163551 None:intergenic 40.0%
TTAATTGCTCTTCACGCGAA+GGG - chr2.3:163802-163821 None:intergenic 40.0%
TTCATTGACGCTCCTGTTAT+TGG - chr2.3:164028-164047 None:intergenic 40.0%
TTGCAGGTGCCTATGTATAT+TGG + chr2.3:163452-163471 MS.gene86993:intron 40.0%
! TTTCACCCTTTTATGAGTGG+TGG + chr2.3:163524-163543 MS.gene86993:CDS 40.0%
!!! CTTTTGGTTCACAATAGCAG+TGG - chr2.3:163133-163152 None:intergenic 40.0%
AAGGAAGATGAAGCCGACAT+AGG + chr2.3:163726-163745 MS.gene86993:intron 45.0%
AGAACTAGCAGGAATTGCAG+TGG + chr2.3:163860-163879 MS.gene86993:CDS 45.0%
AGGAAGATGAAGCCGACATA+GGG + chr2.3:163727-163746 MS.gene86993:intron 45.0%
ATGATCATCATCCTCTCCAC+CGG + chr2.3:163207-163226 MS.gene86993:CDS 45.0%
ATGTAGGTAGGAGAACTAGC+AGG + chr2.3:163849-163868 MS.gene86993:intron 45.0%
CAGATAGGAGCCGAGTTTAT+AGG + chr2.3:163078-163097 MS.gene86993:intron 45.0%
CTGGACCTAGAGTTCTTACT+GGG - chr2.3:164051-164070 None:intergenic 45.0%
CTTTGGTTACATGCTTCCAC+GGG - chr2.3:163296-163315 None:intergenic 45.0%
GAACCAAAAGACACAAGGCT+CGG + chr2.3:163143-163162 MS.gene86993:CDS 45.0%
GAACTAGCAGGAATTGCAGT+GGG + chr2.3:163861-163880 MS.gene86993:CDS 45.0%
GCAAAAGAAATGGTGACTGC+AGG - chr2.3:163258-163277 None:intergenic 45.0%
GCTTTGGTTACATGCTTCCA+CGG - chr2.3:163297-163316 None:intergenic 45.0%
GGGTTTAAGTCTGAGAGAAG+CGG + chr2.3:162764-162783 MS.gene86993:CDS 45.0%
GGTTTAAGTCTGAGAGAAGC+GGG + chr2.3:162765-162784 MS.gene86993:CDS 45.0%
GTGACTCCACCACTCATAAA+AGG - chr2.3:163533-163552 None:intergenic 45.0%
GTGCTGCATTTGCTCTCAAA+GGG + chr2.3:163498-163517 MS.gene86993:CDS 45.0%
GTTAATTGCTCTTCACGCGA+AGG - chr2.3:163803-163822 None:intergenic 45.0%
GTTCATGACAAGTGGGGATA+GGG - chr2.3:163976-163995 None:intergenic 45.0%
TAATTGCTCTTCACGCGAAG+GGG - chr2.3:163801-163820 None:intergenic 45.0%
TCCTGTGCCTCTTGCAAAAA+AGG + chr2.3:162910-162929 MS.gene86993:CDS 45.0%
TGCTGCATTTGCTCTCAAAG+GGG + chr2.3:163499-163518 MS.gene86993:CDS 45.0%
TGTGCTGCATTTGCTCTCAA+AGG + chr2.3:163497-163516 MS.gene86993:CDS 45.0%
TGTTCATGACAAGTGGGGAT+AGG - chr2.3:163977-163996 None:intergenic 45.0%
TTCATGACAAGTGGGGATAG+GGG - chr2.3:163975-163994 None:intergenic 45.0%
! ACTCCTGGTGTACCTCTTTT+TGG + chr2.3:162740-162759 MS.gene86993:CDS 45.0%
!! AATACTTGGAGCTCTAGGTG+GGG + chr2.3:164120-164139 MS.gene86993:CDS 45.0%
!! AATGCTTCGGTGTCCAAGAA+TGG + chr2.3:162831-162850 MS.gene86993:CDS 45.0%
!! CAATACTTGGAGCTCTAGGT+GGG + chr2.3:164119-164138 MS.gene86993:CDS 45.0%
!! CTGGTGTACCTCTTTTTGGT+GGG + chr2.3:162744-162763 MS.gene86993:CDS 45.0%
!!! ACCTTTTTTGCAAGAGGCAC+AGG - chr2.3:162914-162933 None:intergenic 45.0%
!!! TTTTTTGCAAGAGGCACAGG+AGG - chr2.3:162911-162930 None:intergenic 45.0%
AAGGCTCGGAGACTCTGATT+GGG + chr2.3:163157-163176 MS.gene86993:CDS 50.0%
AAGTCTGAGAGAAGCGGGAA+TGG + chr2.3:162770-162789 MS.gene86993:CDS 50.0%
AATGTTCGTTGTCACGGCAG+TGG + chr2.3:163613-163632 MS.gene86993:CDS 50.0%
ACCATATCCTCCGGAAGGAA+CGG - chr2.3:163555-163574 None:intergenic 50.0%
ATTGGGTGTGCTACCTCTAC+TGG + chr2.3:163174-163193 MS.gene86993:CDS 50.0%
CATAACCGCAAGACCAGTAG+AGG - chr2.3:163190-163209 None:intergenic 50.0%
CCACCTAGAGCTCCAAGTAT+TGG - chr2.3:164121-164140 None:intergenic 50.0%
GCTCCAATACTTGGAGCTCT+AGG + chr2.3:164115-164134 MS.gene86993:CDS 50.0%
GCTGGACCTAGAGTTCTTAC+TGG - chr2.3:164052-164071 None:intergenic 50.0%
GGAAGATGAAGCCGACATAG+GGG + chr2.3:163728-163747 MS.gene86993:intron 50.0%
TCATGACAAGTGGGGATAGG+GGG - chr2.3:163974-163993 None:intergenic 50.0%
TCGGCTCCTATCTGTAACAC+AGG - chr2.3:163072-163091 None:intergenic 50.0%
TGAGATGAGCACCAGAGATG+TGG - chr2.3:163233-163252 None:intergenic 50.0%
! CCACCAAAAAGAGGTACACC+AGG - chr2.3:162746-162765 None:intergenic 50.0%
! CCTGGTGTACCTCTTTTTGG+TGG + chr2.3:162743-162762 MS.gene86993:CDS 50.0%
!! CCAATACTTGGAGCTCTAGG+TGG + chr2.3:164118-164137 MS.gene86993:CDS 50.0%
!! TCTCCGAGCCTTGTGTCTTT+TGG - chr2.3:163149-163168 None:intergenic 50.0%
!! TGCTACCTCTACTGGTCTTG+CGG + chr2.3:163182-163201 MS.gene86993:CDS 50.0%
AAGTGGGGATAGGGGGAAGA+AGG - chr2.3:163967-163986 None:intergenic 55.0%
ACCGTTCCTTCCGGAGGATA+TGG + chr2.3:163551-163570 MS.gene86993:CDS 55.0%
ACTCACAGCTCTTGTGTCGG+TGG - chr2.3:163639-163658 None:intergenic 55.0%
AGGGGGTGGCATTAATGAGC+AGG - chr2.3:162888-162907 None:intergenic 55.0%
ATGAGCACCAGAGATGTGGC+CGG - chr2.3:163229-163248 None:intergenic 55.0%
CAAGGCTCGGAGACTCTGAT+TGG + chr2.3:163156-163175 MS.gene86993:CDS 55.0%
CCATCAACACCAGCAACACC+AGG + chr2.3:162716-162735 MS.gene86993:CDS 55.0%
CTGCGTTGCAGGCCAATAAC+AGG + chr2.3:164013-164032 MS.gene86993:intron 55.0%
GCTTGACCATATCCTCCGGA+AGG - chr2.3:163560-163579 None:intergenic 55.0%
! ATGATGGTGCACTGCGTTGC+AGG + chr2.3:164002-164021 MS.gene86993:intron 55.0%
! CTGCATTGAAGCACTTCCCG+TGG + chr2.3:163277-163296 MS.gene86993:CDS 55.0%
! TTGCAAGAGGCACAGGAGGA+GGG - chr2.3:162907-162926 None:intergenic 55.0%
! TTTGCAAGAGGCACAGGAGG+AGG - chr2.3:162908-162927 None:intergenic 55.0%
!! CCTGGTGTTGCTGGTGTTGA+TGG - chr2.3:162719-162738 None:intergenic 55.0%
GGTGGAGTCACCGTTCCTTC+CGG + chr2.3:163542-163561 MS.gene86993:CDS 60.0%
! TGCAAGAGGCACAGGAGGAG+GGG - chr2.3:162906-162925 None:intergenic 60.0%
!! AGAGGTACACCAGGAGTGCC+TGG - chr2.3:162737-162756 None:intergenic 60.0%
AGCACCAGAGATGTGGCCGG+TGG - chr2.3:163226-163245 None:intergenic 65.0%
CAGAGATGTGGCCGGTGGAG+AGG - chr2.3:163221-163240 None:intergenic 65.0%
CCAGCAACACCAGGCACTCC+TGG + chr2.3:162725-162744 MS.gene86993:CDS 65.0%
CTCTCCACCGGCCACATCTC+TGG + chr2.3:163219-163238 MS.gene86993:CDS 65.0%
GGAGTCACCGTTCCTTCCGG+AGG + chr2.3:163545-163564 MS.gene86993:CDS 65.0%
! GCAAGAGGCACAGGAGGAGG+GGG - chr2.3:162905-162924 None:intergenic 65.0%
!! CCAGGAGTGCCTGGTGTTGC+TGG - chr2.3:162728-162747 None:intergenic 65.0%
!! CTTGGAGCTCTAGGTGGGGC+AGG + chr2.3:164124-164143 MS.gene86993:CDS 65.0%
! AGAGGCACAGGAGGAGGGGG+TGG - chr2.3:162902-162921 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 162689 164231 162689 ID=MS.gene86993
chr2.3 mRNA 162689 164231 162689 ID=MS.gene86993.t1;Parent=MS.gene86993
chr2.3 exon 162689 162931 162689 ID=MS.gene86993.t1.exon1;Parent=MS.gene86993.t1
chr2.3 CDS 162689 162931 162689 ID=cds.MS.gene86993.t1;Parent=MS.gene86993.t1
chr2.3 exon 163081 163305 163081 ID=MS.gene86993.t1.exon2;Parent=MS.gene86993.t1
chr2.3 CDS 163081 163305 163081 ID=cds.MS.gene86993.t1;Parent=MS.gene86993.t1
chr2.3 exon 163458 163652 163458 ID=MS.gene86993.t1.exon3;Parent=MS.gene86993.t1
chr2.3 CDS 163458 163652 163458 ID=cds.MS.gene86993.t1;Parent=MS.gene86993.t1
chr2.3 exon 163855 163916 163855 ID=MS.gene86993.t1.exon4;Parent=MS.gene86993.t1
chr2.3 CDS 163855 163916 163855 ID=cds.MS.gene86993.t1;Parent=MS.gene86993.t1
chr2.3 exon 164024 164231 164024 ID=MS.gene86993.t1.exon5;Parent=MS.gene86993.t1
chr2.3 CDS 164024 164231 164024 ID=cds.MS.gene86993.t1;Parent=MS.gene86993.t1
Gene Sequence

>MS.gene86993

ATGGAACATATTAATAATGAGGAAATTCCATCAACACCAGCAACACCAGGCACTCCTGGTGTACCTCTTTTTGGTGGGTTTAAGTCTGAGAGAAGCGGGAATGGTAATAATAAATATAAGAAGTCCCTACTCAAGAATTGTAAATGCTTCGGTGTCCAAGAATGGAACTTAGAAGATGGAGCTCTACCCACAGTTACCTGCTCATTAATGCCACCCCCTCCTCCTGTGCCTCTTGCAAAAAAGGTATATATTACTTTTATTATTATATACATATAATACAACTCTGTATATTTATGTGTGTCTAATCTTTGGTCTAATGTGATCAATGTAGACCATATCATTGAAGTGCTAAGTAGTAGTAATTGATTAATCCAAAACTACCTGTGTTACAGATAGGAGCCGAGTTTATAGGGACATTGATTCTGATGTTTGCTGGGACAGCCACTGCTATTGTGAACCAAAAGACACAAGGCTCGGAGACTCTGATTGGGTGTGCTACCTCTACTGGTCTTGCGGTTATGATCATCATCCTCTCCACCGGCCACATCTCTGGTGCTCATCTCAACCCTGCAGTCACCATTTCTTTTGCTGCATTGAAGCACTTCCCGTGGAAGCATGTAACCAAAGCTTTTCCTTAATCCTTCAGCCTAATTTCCTAATTAAAGTTGTTGAAATTAACATGAATTAATTAAAGCTGTACTTAATTTGCATGTTTAATAAATTATAAGCATGCATGTGTGTAAATATAAAGTATAAATTATAATTGCAGGTGCCTATGTATATTGGTGCACAAGTTTTGGCATCAATATGTGCTGCATTTGCTCTCAAAGGGGTTTTTCACCCTTTTATGAGTGGTGGAGTCACCGTTCCTTCCGGAGGATATGGTCAAGCTTTTGCTTTAGAATTCATTATCAGCTTTAATCTAATGTTCGTTGTCACGGCAGTGGCCACCGACACAAGAGCTGTGAGTATTTGTTCCTATAGTAACTTTTAGCTAATAAATACACAAACTCATTCAAGTTTGAAAAGAAAAAGAGAAGGAAGATGAAGCCGACATAGGGGATAATATCATCCAAATTAAAATTTGAACATATGCTTGGATGAAAAATCCCCTTCGCGTGAAGAGCAATTAACAGAGTCCCTAATTGATGTTTATGGTTATGTAGGTAGGAGAACTAGCAGGAATTGCAGTGGGAGCAACTGTGATGCTCAATATACTAATAGCAGGGTATGTCTATGTCCTAACTCTCACTTATCACTCTCAACATACTCTCTCCTTCTTCCCCCTATCCCCACTTGTCATGAACATATATATGATGGTGCACTGCGTTGCAGGCCAATAACAGGAGCGTCAATGAACCCAGTAAGAACTCTAGGTCCAGCTATAGCTGCAAATAACTACAAAGCCATATGGGTCTATCTCTTAGCTCCAATACTTGGAGCTCTAGGTGGGGCAGGAACTTATACTGCAGTCAAGCTTCCCGAAGAAGATGATAATGCAAAGACTAATGCTTCTTCAAATCACCCCAGCTTTAGAAGATGA
Protein sequence

>MS.gene86993.t1

MEHINNEEIPSTPATPGTPGVPLFGGFKSERSGNGNNKYKKSLLKNCKCFGVQEWNLEDGALPTVTCSLMPPPPPVPLAKKIGAEFIGTLILMFAGTATAIVNQKTQGSETLIGCATSTGLAVMIIILSTGHISGAHLNPAVTISFAALKHFPWKHVPMYIGAQVLASICAAFALKGVFHPFMSGGVTVPSGGYGQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPITGASMNPVRTLGPAIAANNYKAIWVYLLAPILGALGGAGTYTAVKLPEEDDNAKTNASSNHPSFRR