Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88025.t1 | XP_004492881.1 | 40.1 | 177 | 76 | 3 | 25 | 172 | 74 | 249 | 2.20E-16 | 95.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88025.t1 | A0A392LY79 | 48.9 | 182 | 73 | 4 | 12 | 181 | 203 | 376 | 5.1e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene88025 | -0.813019 | 3.16E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene88025 | -0.818007 | 2.45E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene88025 | -0.820739 | 5.85E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene88025 | -0.832767 | 7.83E-56 | -1.69E-46 |
| MS.gene050330 | MS.gene88025 | 0.812549 | 4.01E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene88025 | 0.830963 | 2.18E-55 | -1.69E-46 |
| MS.gene050807 | MS.gene88025 | -0.826628 | 2.44E-54 | -1.69E-46 |
| MS.gene050987 | MS.gene88025 | -0.808127 | 3.61E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene88025 | -0.801101 | 1.06E-48 | -1.69E-46 |
| MS.gene051983 | MS.gene88025 | -0.801293 | 9.64E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene88025 | -0.817331 | 3.49E-52 | -1.69E-46 |
| MS.gene052309 | MS.gene88025 | 0.810363 | 1.20E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene88025 | -0.805014 | 1.64E-49 | -1.69E-46 |
| MS.gene053085 | MS.gene88025 | -0.806051 | 9.92E-50 | -1.69E-46 |
| MS.gene054942 | MS.gene88025 | -0.813886 | 2.04E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene88025 | 0.809008 | 2.34E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene88025 | -0.809737 | 1.63E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene88025 | -0.83197 | 1.23E-55 | -1.69E-46 |
| MS.gene059569 | MS.gene88025 | -0.826189 | 3.11E-54 | -1.69E-46 |
| MS.gene060572 | MS.gene88025 | -0.801811 | 7.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88025.t1 | MTR_7g081150 | 42.000 | 100 | 49 | 2 | 53 | 152 | 212 | 302 | 2.07e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTGATATGGCATGCTTTC+GGG | 0.197077 | 4.3:+69748389 | MS.gene88025:CDS |
| AGAAATCTATGCTTATCTTT+TGG | 0.235647 | 4.3:+69748296 | MS.gene88025:CDS |
| CTGGCAGATTGGCAACTTAT+TGG | 0.248113 | 4.3:-69748452 | None:intergenic |
| TCCTTGCACTTCTAGTGATA+TGG | 0.294087 | 4.3:+69748377 | MS.gene88025:CDS |
| CTAGTGATATGGCATGCTTT+CGG | 0.382639 | 4.3:+69748388 | MS.gene88025:CDS |
| CCGTCCTTGCTCAGGTTGTC+CGG | 0.394563 | 4.3:+69748208 | MS.gene88025:CDS |
| TCTTTCTCTGCGTGATAGAT+TGG | 0.399237 | 4.3:-69747977 | None:intergenic |
| AGTAAGGTTTCAAGGTCTAT+TGG | 0.404978 | 4.3:-69747953 | None:intergenic |
| TGATTGAAGATGCAACAAAA+TGG | 0.411390 | 4.3:-69748320 | None:intergenic |
| ATGTGCAGTCCATTGTTATC+CGG | 0.415512 | 4.3:-69748037 | None:intergenic |
| TACCTGCAATGAAGATGTTT+CGG | 0.417070 | 4.3:+69747674 | None:intergenic |
| CCATATGGCCGTCCTTGCTC+AGG | 0.421763 | 4.3:+69748200 | MS.gene88025:CDS |
| TGCGTGATAGATTGGAAGTA+AGG | 0.424769 | 4.3:-69747969 | None:intergenic |
| TTATTGATCACATCTTCATC+TGG | 0.432963 | 4.3:-69748424 | None:intergenic |
| CCTGAGCAAGGACGGCCATA+TGG | 0.435865 | 4.3:-69748200 | None:intergenic |
| ACAAATAAACACCATAAAAC+CGG | 0.436981 | 4.3:-69748227 | None:intergenic |
| TATGCTGCTCCATATGTTGC+AGG | 0.452102 | 4.3:-69748125 | None:intergenic |
| TATTTGTGCCGTTGCTGCAA+TGG | 0.492504 | 4.3:+69748243 | MS.gene88025:CDS |
| AGATTGGAAGTAAGGTTTCA+AGG | 0.495918 | 4.3:-69747961 | None:intergenic |
| TTGATCACATCTTCATCTGG+TGG | 0.516218 | 4.3:-69748421 | None:intergenic |
| CCGGACAACCTGAGCAAGGA+CGG | 0.520130 | 4.3:-69748208 | None:intergenic |
| GGGATGACCGCCTTTCCATA+TGG | 0.520131 | 4.3:+69748185 | MS.gene88025:CDS |
| ATGTAACATGTTGTCCCTCC+CGG | 0.524389 | 4.3:+69748018 | MS.gene88025:CDS |
| GACAAGAACCATTGCAGCAA+CGG | 0.526821 | 4.3:-69748251 | None:intergenic |
| TGTGCAGTCCATTGTTATCC+GGG | 0.548984 | 4.3:-69748036 | None:intergenic |
| AGGTTTCAAGGTCTATTGGT+AGG | 0.553409 | 4.3:-69747949 | None:intergenic |
| GTGCATAAGATGAAAGCCAT+TGG | 0.555210 | 4.3:-69748075 | None:intergenic |
| ACGCAGAGAAAGACAATCAA+AGG | 0.571451 | 4.3:+69747987 | MS.gene88025:CDS |
| CAGTCCATTGTTATCCGGGA+GGG | 0.596015 | 4.3:-69748032 | None:intergenic |
| GATTTCTGCATACTCGTGAA+AGG | 0.607818 | 4.3:-69748280 | None:intergenic |
| GCAAGGACGGCCATATGGAA+AGG | 0.623069 | 4.3:-69748195 | None:intergenic |
| TTGTCCCTCCCGGATAACAA+TGG | 0.623917 | 4.3:+69748028 | MS.gene88025:CDS |
| TGCGTATGAAAATCATCCAA+TGG | 0.624324 | 4.3:+69748059 | MS.gene88025:CDS |
| AAAACCGGACAACCTGAGCA+AGG | 0.646626 | 4.3:-69748212 | None:intergenic |
| GCATACTCGTGAAAGGTGCA+CGG | 0.652146 | 4.3:-69748273 | None:intergenic |
| GCCATATCACTAGAAGTGCA+AGG | 0.653658 | 4.3:-69748378 | None:intergenic |
| AGGACGGCCATATGGAAAGG+CGG | 0.693360 | 4.3:-69748192 | None:intergenic |
| GCAGTCCATTGTTATCCGGG+AGG | 0.697924 | 4.3:-69748033 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATAACCTTCATGA+TGG | + | chr4.3:69747717-69747736 | MS.gene88025:intron | 20.0% |
| ! | AAATTCATACATAGCATAGA+GGG | - | chr4.3:69747903-69747922 | None:intergenic | 25.0% |
| ! | ACAAATAAACACCATAAAAC+CGG | - | chr4.3:69748230-69748249 | None:intergenic | 25.0% |
| ! | ACAAATAGAAGTCAAATTAG+AGG | - | chr4.3:69747804-69747823 | None:intergenic | 25.0% |
| ! | CAAATAGAAGTCAAATTAGA+GGG | - | chr4.3:69747803-69747822 | None:intergenic | 25.0% |
| ! | TGAAAAAATATCCATCATGA+AGG | - | chr4.3:69747731-69747750 | None:intergenic | 25.0% |
| ! | TTCTATTTGTGTTATGATGT+AGG | + | chr4.3:69747814-69747833 | MS.gene88025:intron | 25.0% |
| !! | AGAAATCTATGCTTATCTTT+TGG | + | chr4.3:69748296-69748315 | MS.gene88025:CDS | 25.0% |
| !! | GAAATCTATGCTTATCTTTT+GGG | + | chr4.3:69748297-69748316 | MS.gene88025:CDS | 25.0% |
| !!! | TTTTTATTTCCTGCAACATA+TGG | + | chr4.3:69748116-69748135 | MS.gene88025:CDS | 25.0% |
| AATTCATACATAGCATAGAG+GGG | - | chr4.3:69747902-69747921 | None:intergenic | 30.0% | |
| GAAATTCATACATAGCATAG+AGG | - | chr4.3:69747904-69747923 | None:intergenic | 30.0% | |
| GATGACATTTATCATTCCTT+TGG | + | chr4.3:69747777-69747796 | MS.gene88025:intron | 30.0% | |
| GGAAATAAAAACAAGGAGAT+TGG | - | chr4.3:69748107-69748126 | None:intergenic | 30.0% | |
| TGATTGAAGATGCAACAAAA+TGG | - | chr4.3:69748323-69748342 | None:intergenic | 30.0% | |
| TGTTGCAGGAAATAAAAACA+AGG | - | chr4.3:69748114-69748133 | None:intergenic | 30.0% | |
| TTATTGATCACATCTTCATC+TGG | - | chr4.3:69748427-69748446 | None:intergenic | 30.0% | |
| AGATTGGAAGTAAGGTTTCA+AGG | - | chr4.3:69747964-69747983 | None:intergenic | 35.0% | |
| AGTAAGGTTTCAAGGTCTAT+TGG | - | chr4.3:69747956-69747975 | None:intergenic | 35.0% | |
| CGGTAACTTAACAATTACTC+TGG | + | chr4.3:69747694-69747713 | MS.gene88025:intron | 35.0% | |
| TGCGTATGAAAATCATCCAA+TGG | + | chr4.3:69748059-69748078 | MS.gene88025:CDS | 35.0% | |
| AAGTCAAATTAGAGGGCCAA+AGG | - | chr4.3:69747796-69747815 | None:intergenic | 40.0% | |
| ACGCAGAGAAAGACAATCAA+AGG | + | chr4.3:69747987-69748006 | MS.gene88025:CDS | 40.0% | |
| AGGTTTCAAGGTCTATTGGT+AGG | - | chr4.3:69747952-69747971 | None:intergenic | 40.0% | |
| ATGTGCAGTCCATTGTTATC+CGG | - | chr4.3:69748040-69748059 | None:intergenic | 40.0% | |
| CTAGTGATATGGCATGCTTT+CGG | + | chr4.3:69748388-69748407 | MS.gene88025:CDS | 40.0% | |
| GATTTCTGCATACTCGTGAA+AGG | - | chr4.3:69748283-69748302 | None:intergenic | 40.0% | |
| GTGCATAAGATGAAAGCCAT+TGG | - | chr4.3:69748078-69748097 | None:intergenic | 40.0% | |
| TAGTGATATGGCATGCTTTC+GGG | + | chr4.3:69748389-69748408 | MS.gene88025:CDS | 40.0% | |
| TCCTTGCACTTCTAGTGATA+TGG | + | chr4.3:69748377-69748396 | MS.gene88025:CDS | 40.0% | |
| TCTTTCTCTGCGTGATAGAT+TGG | - | chr4.3:69747980-69747999 | None:intergenic | 40.0% | |
| TGCGTGATAGATTGGAAGTA+AGG | - | chr4.3:69747972-69747991 | None:intergenic | 40.0% | |
| TTGATCACATCTTCATCTGG+TGG | - | chr4.3:69748424-69748443 | None:intergenic | 40.0% | |
| !!! | TCATCCCATTTTTTGTGGAG+TGG | - | chr4.3:69748172-69748191 | None:intergenic | 40.0% |
| ACATAGCATAGAGGGGTCAA+TGG | - | chr4.3:69747895-69747914 | None:intergenic | 45.0% | |
| ATGTAACATGTTGTCCCTCC+CGG | + | chr4.3:69748018-69748037 | MS.gene88025:CDS | 45.0% | |
| CCCTCCACTCCACAAAAAAT+GGG | + | chr4.3:69748165-69748184 | MS.gene88025:CDS | 45.0% | |
| CTGGCAGATTGGCAACTTAT+TGG | - | chr4.3:69748455-69748474 | None:intergenic | 45.0% | |
| GACAAGAACCATTGCAGCAA+CGG | - | chr4.3:69748254-69748273 | None:intergenic | 45.0% | |
| TATGCTGCTCCATATGTTGC+AGG | - | chr4.3:69748128-69748147 | None:intergenic | 45.0% | |
| TCCCTCCACTCCACAAAAAA+TGG | + | chr4.3:69748164-69748183 | MS.gene88025:CDS | 45.0% | |
| TGTGCAGTCCATTGTTATCC+GGG | - | chr4.3:69748039-69748058 | None:intergenic | 45.0% | |
| ! | GCCATATCACTAGAAGTGCA+AGG | - | chr4.3:69748381-69748400 | None:intergenic | 45.0% |
| ! | TATTTGTGCCGTTGCTGCAA+TGG | + | chr4.3:69748243-69748262 | MS.gene88025:CDS | 45.0% |
| !!! | CCCATTTTTTGTGGAGTGGA+GGG | - | chr4.3:69748168-69748187 | None:intergenic | 45.0% |
| !!! | TCCCATTTTTTGTGGAGTGG+AGG | - | chr4.3:69748169-69748188 | None:intergenic | 45.0% |
| AAAACCGGACAACCTGAGCA+AGG | - | chr4.3:69748215-69748234 | None:intergenic | 50.0% | |
| CAGTCCATTGTTATCCGGGA+GGG | - | chr4.3:69748035-69748054 | None:intergenic | 50.0% | |
| TTGTCCCTCCCGGATAACAA+TGG | + | chr4.3:69748028-69748047 | MS.gene88025:CDS | 50.0% | |
| ! | GCATACTCGTGAAAGGTGCA+CGG | - | chr4.3:69748276-69748295 | None:intergenic | 50.0% |
| ! | TTTTGTGGAGTGGAGGGACA+TGG | - | chr4.3:69748162-69748181 | None:intergenic | 50.0% |
| !! | GCTCAGGTTGTCCGGTTTTA+TGG | + | chr4.3:69748216-69748235 | MS.gene88025:CDS | 50.0% |
| !!! | GGCGGTCATCCCATTTTTTG+TGG | - | chr4.3:69748177-69748196 | None:intergenic | 50.0% |
| AGGACGGCCATATGGAAAGG+CGG | - | chr4.3:69748195-69748214 | None:intergenic | 55.0% | |
| GCAAGGACGGCCATATGGAA+AGG | - | chr4.3:69748198-69748217 | None:intergenic | 55.0% | |
| GCAGTCCATTGTTATCCGGG+AGG | - | chr4.3:69748036-69748055 | None:intergenic | 55.0% | |
| GGGATGACCGCCTTTCCATA+TGG | + | chr4.3:69748185-69748204 | MS.gene88025:CDS | 55.0% | |
| CCATATGGCCGTCCTTGCTC+AGG | + | chr4.3:69748200-69748219 | MS.gene88025:CDS | 60.0% | |
| CCGGACAACCTGAGCAAGGA+CGG | - | chr4.3:69748211-69748230 | None:intergenic | 60.0% | |
| CCTGAGCAAGGACGGCCATA+TGG | - | chr4.3:69748203-69748222 | None:intergenic | 60.0% | |
| ! | CCGTCCTTGCTCAGGTTGTC+CGG | + | chr4.3:69748208-69748227 | MS.gene88025:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 69747682 | 69748483 | 69747682 | ID=MS.gene88025 |
| chr4.3 | mRNA | 69747682 | 69748483 | 69747682 | ID=MS.gene88025.t1;Parent=MS.gene88025 |
| chr4.3 | exon | 69747682 | 69747695 | 69747682 | ID=MS.gene88025.t1.exon1;Parent=MS.gene88025.t1 |
| chr4.3 | CDS | 69747682 | 69747695 | 69747682 | ID=cds.MS.gene88025.t1;Parent=MS.gene88025.t1 |
| chr4.3 | exon | 69747836 | 69747848 | 69747836 | ID=MS.gene88025.t1.exon2;Parent=MS.gene88025.t1 |
| chr4.3 | CDS | 69747836 | 69747848 | 69747836 | ID=cds.MS.gene88025.t1;Parent=MS.gene88025.t1 |
| chr4.3 | exon | 69747926 | 69748483 | 69747926 | ID=MS.gene88025.t1.exon3;Parent=MS.gene88025.t1 |
| chr4.3 | CDS | 69747926 | 69748483 | 69747926 | ID=cds.MS.gene88025.t1;Parent=MS.gene88025.t1 |
| Gene Sequence |
| Protein sequence |
