Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88180.t1 | XP_013455778.1 | 95.1 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.90E-43 | 184.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88180.t1 | A0A072UJ84 | 95.1 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.4e-43 | 184.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050100 | MS.gene88180 | 0.867642 | 1.18E-65 | -1.69E-46 |
| MS.gene050298 | MS.gene88180 | 0.873841 | 1.08E-67 | -1.69E-46 |
| MS.gene05143 | MS.gene88180 | 0.819081 | 1.40E-52 | -1.69E-46 |
| MS.gene052142 | MS.gene88180 | 0.819574 | 1.08E-52 | -1.69E-46 |
| MS.gene052187 | MS.gene88180 | 0.813364 | 2.66E-51 | -1.69E-46 |
| MS.gene053383 | MS.gene88180 | 0.824348 | 8.47E-54 | -1.69E-46 |
| MS.gene055359 | MS.gene88180 | 0.832072 | 1.16E-55 | -1.69E-46 |
| MS.gene057881 | MS.gene88180 | 0.813369 | 2.65E-51 | -1.69E-46 |
| MS.gene059632 | MS.gene88180 | 0.848902 | 4.56E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88180.t1 | MTR_4g052230 | 95.146 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.16e-54 | 165 |
| MS.gene88180.t1 | MTR_2g086870 | 58.974 | 78 | 28 | 2 | 28 | 103 | 1 | 76 | 1.08e-21 | 81.3 |
| MS.gene88180.t1 | MTR_7g105730 | 47.000 | 100 | 51 | 2 | 1 | 99 | 14 | 112 | 3.50e-20 | 78.6 |
| MS.gene88180.t1 | MTR_1g058120 | 41.379 | 116 | 51 | 4 | 1 | 101 | 1 | 114 | 4.58e-18 | 73.2 |
| MS.gene88180.t1 | MTR_2g086850 | 60.976 | 41 | 16 | 0 | 54 | 94 | 70 | 110 | 8.87e-13 | 59.7 |
| MS.gene88180.t1 | MTR_2g086860 | 41.379 | 87 | 40 | 4 | 13 | 90 | 6 | 90 | 9.47e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88180.t1 | AT3G11600 | 46.809 | 94 | 45 | 2 | 1 | 90 | 1 | 93 | 2.36e-20 | 79.3 |
| MS.gene88180.t1 | AT5G06270 | 76.923 | 39 | 9 | 0 | 53 | 91 | 62 | 100 | 2.59e-16 | 68.9 |
| MS.gene88180.t1 | AT5G06270 | 76.923 | 39 | 9 | 0 | 53 | 91 | 62 | 100 | 2.59e-16 | 68.9 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCTTGCACTTGGGACATT+TGG | 0.291863 | 4.4:-50353267 | None:intergenic |
| GGACATTTGGGATCAACTTC+TGG | 0.305242 | 4.4:-50353254 | None:intergenic |
| CTCTTGCACTTGGGACATTT+GGG | 0.346227 | 4.4:-50353266 | None:intergenic |
| TTCAACGTTGGAAGTGGCAG+TGG | 0.382654 | 4.4:+50353061 | MS.gene88180:CDS |
| ACAAAAGCCATGCTTCTTGT+TGG | 0.427150 | 4.4:+50353200 | MS.gene88180:CDS |
| AGCGACGAGGTTGCCGCCCT+TGG | 0.428218 | 4.4:-50353101 | None:intergenic |
| TCGATGCCTCATGTATGTTA+TGG | 0.453292 | 4.4:+50353229 | MS.gene88180:CDS |
| GACGAGGTTGCCGCCCTTGG+TGG | 0.464017 | 4.4:-50353098 | None:intergenic |
| CAACATAGTACTCTTGCACT+TGG | 0.472840 | 4.4:-50353276 | None:intergenic |
| CCAACATGAGTAAAAGATCA+AGG | 0.518273 | 4.4:+50353036 | None:intergenic |
| GGCAACCTCGTCGCTATCAT+CGG | 0.522850 | 4.4:+50353109 | MS.gene88180:CDS |
| AAGGAGTTCAACGTTGGAAG+TGG | 0.536489 | 4.4:+50353055 | MS.gene88180:CDS |
| GCTGAAATTGTCACCACCAA+GGG | 0.539274 | 4.4:+50353085 | MS.gene88180:CDS |
| AACATAGTACTCTTGCACTT+GGG | 0.557195 | 4.4:-50353275 | None:intergenic |
| TCAACGTTGGAAGTGGCAGT+GGG | 0.573815 | 4.4:+50353062 | MS.gene88180:CDS |
| GAATGTGTTCATTCGTCGGA+AGG | 0.586297 | 4.4:+50353146 | MS.gene88180:CDS |
| GACAGAATGTGTTCATTCGT+CGG | 0.592266 | 4.4:+50353142 | MS.gene88180:CDS |
| AAGATCAAGGAGTTCAACGT+TGG | 0.606892 | 4.4:+50353049 | MS.gene88180:CDS |
| GAAATTGTCACCACCAAGGG+CGG | 0.626748 | 4.4:+50353088 | MS.gene88180:CDS |
| GGCTGAAATTGTCACCACCA+AGG | 0.631228 | 4.4:+50353084 | MS.gene88180:CDS |
| AGTACAACCAACAAGAAGCA+TGG | 0.652627 | 4.4:-50353207 | None:intergenic |
| CTGGTACCATAACATACATG+AGG | 0.677492 | 4.4:-50353235 | None:intergenic |
| CAACGTTGGAAGTGGCAGTG+GGG | 0.707130 | 4.4:+50353063 | MS.gene88180:CDS |
| AGATTCCGATGATAGCGACG+AGG | 0.779553 | 4.4:-50353114 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATAGTACTCTTGCACTT+GGG | - | chr4.4:50353278-50353297 | None:intergenic | 35.0% | |
| ! | GCTTGATTTTCTTAACAGTG+AGG | + | chr4.4:50353298-50353317 | MS.gene88180:CDS | 35.0% |
| AAGATCAAGGAGTTCAACGT+TGG | + | chr4.4:50353049-50353068 | MS.gene88180:CDS | 40.0% | |
| ACAAAAGCCATGCTTCTTGT+TGG | + | chr4.4:50353200-50353219 | MS.gene88180:CDS | 40.0% | |
| AGTACAACCAACAAGAAGCA+TGG | - | chr4.4:50353210-50353229 | None:intergenic | 40.0% | |
| CAACATAGTACTCTTGCACT+TGG | - | chr4.4:50353279-50353298 | None:intergenic | 40.0% | |
| CTGGTACCATAACATACATG+AGG | - | chr4.4:50353238-50353257 | None:intergenic | 40.0% | |
| GACAGAATGTGTTCATTCGT+CGG | + | chr4.4:50353142-50353161 | MS.gene88180:CDS | 40.0% | |
| TCGATGCCTCATGTATGTTA+TGG | + | chr4.4:50353229-50353248 | MS.gene88180:CDS | 40.0% | |
| AAGGAGTTCAACGTTGGAAG+TGG | + | chr4.4:50353055-50353074 | MS.gene88180:CDS | 45.0% | |
| ACTCTTGCACTTGGGACATT+TGG | - | chr4.4:50353270-50353289 | None:intergenic | 45.0% | |
| CTCTTGCACTTGGGACATTT+GGG | - | chr4.4:50353269-50353288 | None:intergenic | 45.0% | |
| GAATGTGTTCATTCGTCGGA+AGG | + | chr4.4:50353146-50353165 | MS.gene88180:CDS | 45.0% | |
| GCTGAAATTGTCACCACCAA+GGG | + | chr4.4:50353085-50353104 | MS.gene88180:CDS | 45.0% | |
| ! | GGACATTTGGGATCAACTTC+TGG | - | chr4.4:50353257-50353276 | None:intergenic | 45.0% |
| GAAATTGTCACCACCAAGGG+CGG | + | chr4.4:50353088-50353107 | MS.gene88180:CDS | 50.0% | |
| GGCTGAAATTGTCACCACCA+AGG | + | chr4.4:50353084-50353103 | MS.gene88180:CDS | 50.0% | |
| TCAACGTTGGAAGTGGCAGT+GGG | + | chr4.4:50353062-50353081 | MS.gene88180:CDS | 50.0% | |
| TTCAACGTTGGAAGTGGCAG+TGG | + | chr4.4:50353061-50353080 | MS.gene88180:CDS | 50.0% | |
| ! | AGATTCCGATGATAGCGACG+AGG | - | chr4.4:50353117-50353136 | None:intergenic | 50.0% |
| CAACGTTGGAAGTGGCAGTG+GGG | + | chr4.4:50353063-50353082 | MS.gene88180:CDS | 55.0% | |
| GGCAACCTCGTCGCTATCAT+CGG | + | chr4.4:50353109-50353128 | MS.gene88180:CDS | 55.0% | |
| AGCGACGAGGTTGCCGCCCT+TGG | - | chr4.4:50353104-50353123 | None:intergenic | 70.0% | |
| GACGAGGTTGCCGCCCTTGG+TGG | - | chr4.4:50353101-50353120 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 50353041 | 50353352 | 50353041 | ID=MS.gene88180 |
| chr4.4 | mRNA | 50353041 | 50353352 | 50353041 | ID=MS.gene88180.t1;Parent=MS.gene88180 |
| chr4.4 | exon | 50353041 | 50353352 | 50353041 | ID=MS.gene88180.t1.exon1;Parent=MS.gene88180.t1 |
| chr4.4 | CDS | 50353041 | 50353352 | 50353041 | ID=cds.MS.gene88180.t1;Parent=MS.gene88180.t1 |
| Gene Sequence |
| Protein sequence |
