AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89034

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89034.t1 MTR_2g103600 97.468 316 8 0 1 316 1 316 0.0 625
MS.gene89034.t1 MTR_4g021755 56.184 283 114 5 30 310 41 315 2.20e-101 303
MS.gene89034.t1 MTR_7g498280 56.357 291 118 4 15 304 20 302 1.09e-100 299
MS.gene89034.t1 MTR_7g061780 54.255 282 120 4 30 310 25 298 1.76e-95 286
MS.gene89034.t1 MTR_2g097210 55.636 275 114 3 30 302 43 311 2.48e-90 275
MS.gene89034.t1 MTR_2g097210 56.818 264 106 3 30 291 43 300 2.92e-89 269
MS.gene89034.t1 MTR_1g101310 48.366 306 138 3 3 304 5 294 1.33e-88 268
MS.gene89034.t1 MTR_3g073530 43.985 266 141 3 33 297 42 300 1.69e-55 185
MS.gene89034.t1 MTR_6g089790 68.421 57 16 1 191 247 1 55 6.01e-17 74.3
MS.gene89034.t1 MTR_2g088315 46.053 76 36 1 107 182 13 83 1.63e-15 70.9
MS.gene89034.t1 MTR_2g088290 30.435 207 90 8 51 252 1 158 2.08e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89034.t1 AT1G52580 60.797 301 109 7 1 299 1 294 4.19e-120 348
MS.gene89034.t1 AT2G29050 61.011 277 99 4 27 302 50 318 1.26e-108 321
MS.gene89034.t1 AT2G29050 55.932 295 93 5 27 292 50 336 1.55e-102 304
MS.gene89034.t1 AT1G63120 57.312 253 96 4 28 278 28 270 1.21e-97 291
MS.gene89034.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene89034.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene89034.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene89034.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene89034.t1 AT1G12750 51.786 280 126 4 29 306 16 288 9.48e-94 281
MS.gene89034.t1 AT5G07250 50.649 308 132 5 11 306 25 324 6.12e-90 272
MS.gene89034.t1 AT3G53780 50.554 271 122 4 30 300 63 321 2.44e-84 259
MS.gene89034.t1 AT3G53780 51.145 262 116 4 30 291 63 312 2.97e-83 254
MS.gene89034.t1 AT4G23070 51.971 279 123 5 27 304 25 293 8.39e-75 233
MS.gene89034.t1 AT1G12750 55.615 187 73 4 29 211 16 196 9.79e-66 206
MS.gene89034.t1 AT1G12750 56.983 179 69 3 29 205 16 188 1.20e-65 205
MS.gene89034.t1 AT3G53780 50.000 206 94 2 95 300 1 197 3.82e-64 204
MS.gene89034.t1 AT1G77860 41.132 265 136 5 33 290 48 299 1.89e-59 194
MS.gene89034.t1 AT1G77860 41.132 265 136 5 33 290 48 299 2.71e-59 195
MS.gene89034.t1 AT5G07250 53.140 207 79 4 11 205 25 225 2.60e-58 189
MS.gene89034.t1 AT1G25290 34.375 160 91 5 103 255 161 313 1.75e-14 73.6
MS.gene89034.t1 AT1G25290 34.416 154 92 4 103 255 161 306 3.24e-14 72.8
MS.gene89034.t1 AT5G38510 29.193 161 105 4 96 254 219 372 1.09e-11 65.5
MS.gene89034.t1 AT5G38510 29.193 161 105 4 96 254 219 372 1.09e-11 65.5
MS.gene89034.t1 AT5G38510 29.193 161 105 4 96 254 219 372 1.09e-11 65.5

Find 80 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCTTTGGTATCAATATT+TGG 0.097106 2.4:+1958070 MS.gene89034:CDS
GGAGTTCTATATTTGCTTTC+TGG 0.112592 2.4:+1958966 MS.gene89034:CDS
GTTCGCCTTGAGAATGAATT+TGG 0.205071 2.4:+1958820 MS.gene89034:CDS
TCTCATCATTGGCCTAAATT+TGG 0.218525 2.4:+1959516 MS.gene89034:CDS
CTATATTTGCTTTCTGGCTT+TGG 0.237472 2.4:+1958972 MS.gene89034:CDS
TTGGATTTGGCAGTGAAAAT+TGG 0.260847 2.4:+1958945 MS.gene89034:intron
GCACCAACTGATACGGTATT+TGG 0.268428 2.4:-1959038 None:intergenic
TTGGACTATCTATTTAAATA+AGG 0.284285 2.4:+1959115 MS.gene89034:CDS
ATACACGAACATTGCAATAT+TGG 0.288693 2.4:-1958003 None:intergenic
TTCTGCAGGTTGAGGGTATT+AGG 0.315192 2.4:+1958676 MS.gene89034:intron
TAAATCCAACAGCCAAATTT+AGG 0.316777 2.4:-1959528 None:intergenic
TGCCTTTGGTATCAATATTT+GGG 0.351007 2.4:+1958071 MS.gene89034:CDS
AGAAAGTAGGCGGTGGTGGC+GGG 0.367689 2.4:-1957926 None:intergenic
TCAGTCATTACGCTACTTAT+CGG 0.370474 2.4:+1959727 MS.gene89034:CDS
AAACGTAAATCCAAGTATAA+AGG 0.381415 2.4:+1959679 MS.gene89034:CDS
TAGAAAGTAGGCGGTGGTGG+CGG 0.386984 2.4:-1957927 None:intergenic
CATATAGGAGGATTCCTATC+AGG 0.389877 2.4:+1959574 MS.gene89034:CDS
ACCGTATCAGTTGGTGCATC+GGG 0.403381 2.4:+1959044 MS.gene89034:CDS
GAATAAAAGACTAAACATAT+TGG 0.408803 2.4:-1958791 None:intergenic
TATTTGCTTTCTGGCTTTGG+TGG 0.424233 2.4:+1958975 MS.gene89034:CDS
ACTTGCATGTTTCTTCATGC+AGG 0.433987 2.4:+1958751 MS.gene89034:CDS
AGGCTATCAGTATTTCTTCC+TGG 0.441269 2.4:+1959699 MS.gene89034:CDS
ACTTTCAGTATTCGCTAAGA+AGG 0.444351 2.4:-1960106 None:intergenic
GTTTACATTCTGCAGGTTGA+GGG 0.447403 2.4:+1958669 MS.gene89034:intron
GAAAGTAGGCGGTGGTGGCG+GGG 0.449589 2.4:-1957925 None:intergenic
ATTTAAATAGATAGTCCAAT+TGG 0.450252 2.4:-1959111 None:intergenic
CAGTCATTACGCTACTTATC+GGG 0.457261 2.4:+1959728 MS.gene89034:CDS
ATAACGAGCCGCCTCAATAT+CGG 0.458145 2.4:-1957901 None:intergenic
ATACTGATAGCCTTTATACT+TGG 0.460587 2.4:-1959689 None:intergenic
TTGTTCACATAACCAAATTG+AGG 0.462978 2.4:-1959628 None:intergenic
ATTGGCCTAAATTTGGCTGT+TGG 0.471687 2.4:+1959523 MS.gene89034:CDS
AAAGTAGGCGGTGGTGGCGG+GGG 0.473559 2.4:-1957924 None:intergenic
TCATCTTCATTCAAATAGCC+AGG 0.474228 2.4:-1958047 None:intergenic
TTAGTGAGCTTCTAACCAAT+TGG 0.474947 2.4:+1959096 MS.gene89034:CDS
TACCGTATCAGTTGGTGCAT+CGG 0.475291 2.4:+1959043 MS.gene89034:CDS
ACAAGTCTACTTCTCATCAT+TGG 0.480296 2.4:+1959505 MS.gene89034:CDS
TTCAATGAAAACCCTCTCCT+TGG 0.483625 2.4:+1958113 MS.gene89034:CDS
ATGTAGAAAGTAGGCGGTGG+TGG 0.484370 2.4:-1957930 None:intergenic
TGGTGGGTGAAAATGAAGTA+TGG 0.484851 2.4:+1958719 MS.gene89034:CDS
TGAATGAAGATGATTGCCTT+TGG 0.484862 2.4:+1958057 MS.gene89034:CDS
TCATTTGACAGATATGCCTA+TGG 0.495016 2.4:+1960042 MS.gene89034:intron
TGTTCACATAACCAAATTGA+GGG 0.495056 2.4:-1959627 None:intergenic
CGCCAACACAATGGTTCTCA+TGG 0.513113 2.4:+1957958 MS.gene89034:CDS
GATTCTCACGTGCGGACAGA+AGG 0.516174 2.4:-1958137 None:intergenic
AAAATCCAAATTCATTCTCA+AGG 0.516279 2.4:-1958825 None:intergenic
GTGGACAATTCAGCTCATAT+AGG 0.523918 2.4:+1959559 MS.gene89034:CDS
AACACAATGGTTCTCATGGT+TGG 0.531112 2.4:+1957962 MS.gene89034:CDS
GGACAGAAGGGCCAAGGAGA+GGG 0.532109 2.4:-1958124 None:intergenic
CATGAGAACCATTGTGTTGG+CGG 0.546872 2.4:-1957957 None:intergenic
ACCCGATGCACCAACTGATA+CGG 0.550141 2.4:-1959045 None:intergenic
GTCGCCGTTCTCCGATATTG+AGG 0.551647 2.4:+1957890 MS.gene89034:CDS
GGTATTAGGAGCTCTTGAAA+AGG 0.553959 2.4:+1958690 MS.gene89034:CDS
TTCCCAAATATTGATACCAA+AGG 0.563541 2.4:-1958073 None:intergenic
ACGTGCGGACAGAAGGGCCA+AGG 0.568851 2.4:-1958130 None:intergenic
ATGTATATCAATGATTGCCC+TGG 0.571703 2.4:+1958029 MS.gene89034:CDS
AACCATGAGAACCATTGTGT+TGG 0.576399 2.4:-1957960 None:intergenic
TCTTGAAAAGGACCTTGTGG+TGG 0.579909 2.4:+1958702 MS.gene89034:CDS
GTACAATGTAGCTAAGCCAT+AGG 0.586121 2.4:-1960058 None:intergenic
GGCGGTGGCATGTAGAAAGT+AGG 0.588387 2.4:-1957939 None:intergenic
GACAATTCAGCTCATATAGG+AGG 0.594629 2.4:+1959562 MS.gene89034:CDS
GGTTAGAAGCTCACTAAGCA+TGG 0.598778 2.4:-1959090 None:intergenic
GCGTAATGACTGACAAGACC+AGG 0.609130 2.4:-1959717 None:intergenic
ATTCTCACGTGCGGACAGAA+GGG 0.610517 2.4:-1958136 None:intergenic
CGGACAGAAGGGCCAAGGAG+AGG 0.611548 2.4:-1958125 None:intergenic
CATCTTCATTCAAATAGCCA+GGG 0.618324 2.4:-1958046 None:intergenic
GCCGCCTCAATATCGGAGAA+CGG 0.618640 2.4:-1957894 None:intergenic
GAGAACCATTGTGTTGGCGG+TGG 0.622152 2.4:-1957954 None:intergenic
GCACCAAATACCGTATCAGT+TGG 0.622766 2.4:+1959035 MS.gene89034:CDS
GGCTTAGCTACATTGTACAT+AGG 0.638607 2.4:+1960063 MS.gene89034:CDS
AGCTCTTGAAAAGGACCTTG+TGG 0.646384 2.4:+1958699 MS.gene89034:CDS
TGAGCTGAATTGTCCACATG+AGG 0.649223 2.4:-1959553 None:intergenic
GGCATGTAGAAAGTAGGCGG+TGG 0.649400 2.4:-1957933 None:intergenic
CTTGAAAAGGACCTTGTGGT+GGG 0.656591 2.4:+1958703 MS.gene89034:CDS
AACAACAAATATATACCACC+AGG 0.669042 2.4:+1959646 MS.gene89034:CDS
GCCGTTCTCCGATATTGAGG+CGG 0.669086 2.4:+1957893 MS.gene89034:CDS
ACATGCCACCGCCAACACAA+TGG 0.675117 2.4:+1957949 MS.gene89034:CDS
TGTTGGATTTATACCTCATG+TGG 0.677933 2.4:+1959540 MS.gene89034:CDS
GCATTAAAATAACAAAACCA+AGG 0.678293 2.4:-1959603 None:intergenic
GGTGGCATGTAGAAAGTAGG+CGG 0.681757 2.4:-1957936 None:intergenic
TGGGTGAAAATGAAGTATGG+CGG 0.777515 2.4:+1958722 MS.gene89034:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAAAAAAAAATACATA+GGG + chr2.4:1959172-1959191 MS.gene89034:intron 10.0%
!! TTACTTATTATATTTATTCT+TGG + chr2.4:1958926-1958945 MS.gene89034:intron 10.0%
!! AATTTGAATTTAATCTGTAT+TGG + chr2.4:1959989-1960008 MS.gene89034:intron 15.0%
!! ATTATATTTATTCTTGGATT+TGG + chr2.4:1958932-1958951 MS.gene89034:intron 15.0%
!! GAAAGAAAAAAAAAATACAT+AGG + chr2.4:1959171-1959190 MS.gene89034:intron 15.0%
!! AAAAAAAAAATACATAGGGT+GGG + chr2.4:1959176-1959195 MS.gene89034:intron 20.0%
!! ATTTAAATAGATAGTCCAAT+TGG - chr2.4:1959114-1959133 None:intergenic 20.0%
!! GAATAAAAGACTAAACATAT+TGG - chr2.4:1958794-1958813 None:intergenic 20.0%
!! TCAAAGTTAATCTTGTAAAT+TGG + chr2.4:1959268-1959287 MS.gene89034:intron 20.0%
!! TTGGACTATCTATTTAAATA+AGG + chr2.4:1959115-1959134 MS.gene89034:CDS 20.0%
!! TTTATGTTCAATCATAATGA+TGG + chr2.4:1959966-1959985 MS.gene89034:intron 20.0%
!!! ATGTTTAGTCTTTTATTCAT+AGG + chr2.4:1958796-1958815 MS.gene89034:CDS 20.0%
!!! CAAACGTTTTTAATAATTTG+AGG + chr2.4:1959875-1959894 MS.gene89034:intron 20.0%
!!! CATCATCATTTTAAATATCA+TGG + chr2.4:1958193-1958212 MS.gene89034:intron 20.0%
!!! TTGTTATCTATTTTGCATTT+GGG + chr2.4:1959002-1959021 MS.gene89034:CDS 20.0%
!!! TTTGTTATCTATTTTGCATT+TGG + chr2.4:1959001-1959020 MS.gene89034:CDS 20.0%
! AAAATCCAAATTCATTCTCA+AGG - chr2.4:1958828-1958847 None:intergenic 25.0%
! AAACGTAAATCCAAGTATAA+AGG + chr2.4:1959679-1959698 MS.gene89034:CDS 25.0%
! ATTACATCAAAAGTTAAAGG+AGG - chr2.4:1960019-1960038 None:intergenic 25.0%
! GAAAAAAAAAATACATAGGG+TGG + chr2.4:1959175-1959194 MS.gene89034:intron 25.0%
! GCAATTACATCAAAAGTTAA+AGG - chr2.4:1960022-1960041 None:intergenic 25.0%
! GCATTAAAATAACAAAACCA+AGG - chr2.4:1959606-1959625 None:intergenic 25.0%
! GCTTTCTCATTAAAATATCA+TGG + chr2.4:1958239-1958258 MS.gene89034:intron 25.0%
! TCATAAGCTTGATTATAAAG+AGG + chr2.4:1959315-1959334 MS.gene89034:intron 25.0%
! TGCCGAAATATAAAAAATTG+CGG + chr2.4:1958482-1958501 MS.gene89034:intron 25.0%
!! ATATTGGCTAAAAAAATGAG+TGG - chr2.4:1957990-1958009 None:intergenic 25.0%
!!! ATCTTTTTACTTTTGAACCA+AGG + chr2.4:1959425-1959444 MS.gene89034:intron 25.0%
!!! CACCGCAATTTTTTATATTT+CGG - chr2.4:1958487-1958506 None:intergenic 25.0%
!!! TGAAATGATAGTGATTTGTT+TGG - chr2.4:1959821-1959840 None:intergenic 25.0%
!!! TTTGTCAATTGGTTGTTTTA+GGG + chr2.4:1958639-1958658 MS.gene89034:intron 25.0%
!!! TTTTGTCAATTGGTTGTTTT+AGG + chr2.4:1958638-1958657 MS.gene89034:intron 25.0%
AAACAACCAATTGACAAAAG+AGG - chr2.4:1958637-1958656 None:intergenic 30.0%
AACAACAAATATATACCACC+AGG + chr2.4:1959646-1959665 MS.gene89034:CDS 30.0%
ATAATGTTCAACAATGTACC+TGG + chr2.4:1958217-1958236 MS.gene89034:intron 30.0%
ATACACGAACATTGCAATAT+TGG - chr2.4:1958006-1958025 None:intergenic 30.0%
ATACTGATAGCCTTTATACT+TGG - chr2.4:1959692-1959711 None:intergenic 30.0%
ATTTGAGGACTTTGATTGAT+TGG + chr2.4:1959890-1959909 MS.gene89034:intron 30.0%
CTTCTATTGTCTTACTTAGA+TGG + chr2.4:1958530-1958549 MS.gene89034:intron 30.0%
TAAATCCAACAGCCAAATTT+AGG - chr2.4:1959531-1959550 None:intergenic 30.0%
TCAAAAACACAGAGACAATT+TGG + chr2.4:1959355-1959374 MS.gene89034:intron 30.0%
TGCAGCTTAACAAAAAATTG+AGG + chr2.4:1958410-1958429 MS.gene89034:intron 30.0%
TGCCTTTGGTATCAATATTT+GGG + chr2.4:1958071-1958090 MS.gene89034:CDS 30.0%
TGTTCACATAACCAAATTGA+GGG - chr2.4:1959630-1959649 None:intergenic 30.0%
TTATTGTGTTTACATTCTGC+AGG + chr2.4:1958662-1958681 MS.gene89034:intron 30.0%
TTCCCAAATATTGATACCAA+AGG - chr2.4:1958076-1958095 None:intergenic 30.0%
TTGCCTTTGGTATCAATATT+TGG + chr2.4:1958070-1958089 MS.gene89034:CDS 30.0%
TTGTTCACATAACCAAATTG+AGG - chr2.4:1959631-1959650 None:intergenic 30.0%
! TTTCATCCTCTTTTGTCAAT+TGG + chr2.4:1958628-1958647 MS.gene89034:intron 30.0%
! TTTTATATTTCGGCAAAACG+CGG - chr2.4:1958477-1958496 None:intergenic 30.0%
!! AATGATAGTGATTTGTTTGG+CGG - chr2.4:1959818-1959837 None:intergenic 30.0%
!!! GATATTTTAATGAGAAAGCC+AGG - chr2.4:1958238-1958257 None:intergenic 30.0%
AACCAAGGAAAAATCCTGAT+AGG - chr2.4:1959591-1959610 None:intergenic 35.0%
ACAAGTCTACTTCTCATCAT+TGG + chr2.4:1959505-1959524 MS.gene89034:CDS 35.0%
ACTTTCAGTATTCGCTAAGA+AGG - chr2.4:1960109-1960128 None:intergenic 35.0%
ACTTTGAACTGTACTGTGTA+AGG - chr2.4:1959255-1959274 None:intergenic 35.0%
ATGTATATCAATGATTGCCC+TGG + chr2.4:1958029-1958048 MS.gene89034:CDS 35.0%
ATGTGAGAGAGAAATTGAGA+GGG - chr2.4:1958167-1958186 None:intergenic 35.0%
ATTATGTCAAATGCATGTCC+TGG + chr2.4:1958341-1958360 MS.gene89034:intron 35.0%
CATCTTCATTCAAATAGCCA+GGG - chr2.4:1958049-1958068 None:intergenic 35.0%
CTTTGAACTGTACTGTGTAA+GGG - chr2.4:1959254-1959273 None:intergenic 35.0%
GGAGTTCTATATTTGCTTTC+TGG + chr2.4:1958966-1958985 MS.gene89034:CDS 35.0%
TATGTGAGAGAGAAATTGAG+AGG - chr2.4:1958168-1958187 None:intergenic 35.0%
TCAGTCATTACGCTACTTAT+CGG + chr2.4:1959727-1959746 MS.gene89034:CDS 35.0%
TCATCTTCATTCAAATAGCC+AGG - chr2.4:1958050-1958069 None:intergenic 35.0%
TCATTTGACAGATATGCCTA+TGG + chr2.4:1960042-1960061 MS.gene89034:intron 35.0%
TGAATGAAGATGATTGCCTT+TGG + chr2.4:1958057-1958076 MS.gene89034:CDS 35.0%
TGTTGGATTTATACCTCATG+TGG + chr2.4:1959540-1959559 MS.gene89034:CDS 35.0%
TTAGTGAGCTTCTAACCAAT+TGG + chr2.4:1959096-1959115 MS.gene89034:CDS 35.0%
TTGGATTTGGCAGTGAAAAT+TGG + chr2.4:1958945-1958964 MS.gene89034:intron 35.0%
TTTGAACTGTACTGTGTAAG+GGG - chr2.4:1959253-1959272 None:intergenic 35.0%
! TCTCATCATTGGCCTAAATT+TGG + chr2.4:1959516-1959535 MS.gene89034:CDS 35.0%
! TTACGTTTTGCATCATATCC+TGG - chr2.4:1959667-1959686 None:intergenic 35.0%
! TTCCTATCAGGATTTTTCCT+TGG + chr2.4:1959586-1959605 MS.gene89034:CDS 35.0%
!! ATTTTAATGCGCCCTCAATT+TGG + chr2.4:1959616-1959635 MS.gene89034:CDS 35.0%
!! CTATATTTGCTTTCTGGCTT+TGG + chr2.4:1958972-1958991 MS.gene89034:CDS 35.0%
!!! ACATTTTAAAACCCCATGCA+TGG - chr2.4:1958374-1958393 None:intergenic 35.0%
!!! ATGCATGGGGTTTTAAAATG+TGG + chr2.4:1958373-1958392 MS.gene89034:intron 35.0%
AACACAATGGTTCTCATGGT+TGG + chr2.4:1957962-1957981 MS.gene89034:CDS 40.0%
AACCATGAGAACCATTGTGT+TGG - chr2.4:1957963-1957982 None:intergenic 40.0%
ACTTGCATGTTTCTTCATGC+AGG + chr2.4:1958751-1958770 MS.gene89034:CDS 40.0%
AGGCTATCAGTATTTCTTCC+TGG + chr2.4:1959699-1959718 MS.gene89034:CDS 40.0%
ATCTGTTAGAACAACAGCCT+TGG - chr2.4:1959445-1959464 None:intergenic 40.0%
CAGTCATTACGCTACTTATC+GGG + chr2.4:1959728-1959747 MS.gene89034:CDS 40.0%
CATATAGGAGGATTCCTATC+AGG + chr2.4:1959574-1959593 MS.gene89034:CDS 40.0%
CCTACAATACATGCATGCAT+AGG + chr2.4:1958582-1958601 MS.gene89034:intron 40.0%
CCTATGCATGCATGTATTGT+AGG - chr2.4:1958585-1958604 None:intergenic 40.0%
GACAATTCAGCTCATATAGG+AGG + chr2.4:1959562-1959581 MS.gene89034:CDS 40.0%
GCACACTGTAGAAGTAAACA+TGG - chr2.4:1959478-1959497 None:intergenic 40.0%
GGCTTAGCTACATTGTACAT+AGG + chr2.4:1960063-1960082 MS.gene89034:CDS 40.0%
GGTATTAGGAGCTCTTGAAA+AGG + chr2.4:1958690-1958709 MS.gene89034:CDS 40.0%
GTACAATGTAGCTAAGCCAT+AGG - chr2.4:1960061-1960080 None:intergenic 40.0%
GTGGACAATTCAGCTCATAT+AGG + chr2.4:1959559-1959578 MS.gene89034:CDS 40.0%
GTTCGCCTTGAGAATGAATT+TGG + chr2.4:1958820-1958839 MS.gene89034:CDS 40.0%
TGGGTGAAAATGAAGTATGG+CGG + chr2.4:1958722-1958741 MS.gene89034:CDS 40.0%
TGTTTACATTCTGCAGGTTG+AGG + chr2.4:1958668-1958687 MS.gene89034:intron 40.0%
TTCAATGAAAACCCTCTCCT+TGG + chr2.4:1958113-1958132 MS.gene89034:CDS 40.0%
! AGGAGAGGGTTTTCATTGAA+AGG - chr2.4:1958113-1958132 None:intergenic 40.0%
! ATTGGCCTAAATTTGGCTGT+TGG + chr2.4:1959523-1959542 MS.gene89034:CDS 40.0%
! GTTTACATTCTGCAGGTTGA+GGG + chr2.4:1958669-1958688 MS.gene89034:intron 40.0%
! TGGTGGGTGAAAATGAAGTA+TGG + chr2.4:1958719-1958738 MS.gene89034:CDS 40.0%
!! TATTTGCTTTCTGGCTTTGG+TGG + chr2.4:1958975-1958994 MS.gene89034:CDS 40.0%
AGCTCTTGAAAAGGACCTTG+TGG + chr2.4:1958699-1958718 MS.gene89034:CDS 45.0%
ATAACGAGCCGCCTCAATAT+CGG - chr2.4:1957904-1957923 None:intergenic 45.0%
CATGAGAACCATTGTGTTGG+CGG - chr2.4:1957960-1957979 None:intergenic 45.0%
CCTGGATTACTTCCATGCAT+GGG + chr2.4:1958359-1958378 MS.gene89034:intron 45.0%
CTGGATTACTTCCATGCATG+GGG + chr2.4:1958360-1958379 MS.gene89034:intron 45.0%
CTTGAAAAGGACCTTGTGGT+GGG + chr2.4:1958703-1958722 MS.gene89034:CDS 45.0%
GCACCAAATACCGTATCAGT+TGG + chr2.4:1959035-1959054 MS.gene89034:CDS 45.0%
GCACCAACTGATACGGTATT+TGG - chr2.4:1959041-1959060 None:intergenic 45.0%
GGTTAGAAGCTCACTAAGCA+TGG - chr2.4:1959093-1959112 None:intergenic 45.0%
TCCTGGATTACTTCCATGCA+TGG + chr2.4:1958358-1958377 MS.gene89034:intron 45.0%
TCTTGAAAAGGACCTTGTGG+TGG + chr2.4:1958702-1958721 MS.gene89034:CDS 45.0%
TGAGCTGAATTGTCCACATG+AGG - chr2.4:1959556-1959575 None:intergenic 45.0%
! CGTTTTGCATCATATCCTGG+TGG - chr2.4:1959664-1959683 None:intergenic 45.0%
! CTTCATTTTCACCCACCACA+AGG - chr2.4:1958717-1958736 None:intergenic 45.0%
! TACCGTATCAGTTGGTGCAT+CGG + chr2.4:1959043-1959062 MS.gene89034:CDS 45.0%
! TCACGGAACACTGTCCTTTT+TGG - chr2.4:1959208-1959227 None:intergenic 45.0%
!! CTCTGTGTTTTTGACCGTAG+AGG - chr2.4:1959349-1959368 None:intergenic 45.0%
!! GGTGCACTTTTTGGACTTCT+GGG + chr2.4:1959065-1959084 MS.gene89034:CDS 45.0%
!! TTCTGCAGGTTGAGGGTATT+AGG + chr2.4:1958676-1958695 MS.gene89034:intron 45.0%
AAGGACAGTGTTCCGTGACT+CGG + chr2.4:1959210-1959229 MS.gene89034:intron 50.0%
ACCCGATGCACCAACTGATA+CGG - chr2.4:1959048-1959067 None:intergenic 50.0%
AGGGAGAAGATTCTCACGTG+CGG - chr2.4:1958148-1958167 None:intergenic 50.0%
ATGTAGAAAGTAGGCGGTGG+TGG - chr2.4:1957933-1957952 None:intergenic 50.0%
ATTCTCACGTGCGGACAGAA+GGG - chr2.4:1958139-1958158 None:intergenic 50.0%
CCCATGCATGGAAGTAATCC+AGG - chr2.4:1958362-1958381 None:intergenic 50.0%
CGCCAACACAATGGTTCTCA+TGG + chr2.4:1957958-1957977 MS.gene89034:CDS 50.0%
GCGTAATGACTGACAAGACC+AGG - chr2.4:1959720-1959739 None:intergenic 50.0%
GGTGGCATGTAGAAAGTAGG+CGG - chr2.4:1957939-1957958 None:intergenic 50.0%
! ACCGTATCAGTTGGTGCATC+GGG + chr2.4:1959044-1959063 MS.gene89034:CDS 50.0%
!! AAGAGGCGTTGTAGCCTCTA+CGG + chr2.4:1959332-1959351 MS.gene89034:intron 50.0%
!! GGGTGCACTTTTTGGACTTC+TGG + chr2.4:1959064-1959083 MS.gene89034:CDS 50.0%
ACATGCCACCGCCAACACAA+TGG + chr2.4:1957949-1957968 MS.gene89034:CDS 55.0%
AGGACAGTGTTCCGTGACTC+GGG + chr2.4:1959211-1959230 MS.gene89034:intron 55.0%
AGGGTGGGTGTGTGCCAAAA+AGG + chr2.4:1959191-1959210 MS.gene89034:intron 55.0%
GATTCTCACGTGCGGACAGA+AGG - chr2.4:1958140-1958159 None:intergenic 55.0%
GCCGCCTCAATATCGGAGAA+CGG - chr2.4:1957897-1957916 None:intergenic 55.0%
GCCGTTCTCCGATATTGAGG+CGG + chr2.4:1957893-1957912 MS.gene89034:CDS 55.0%
GGCATGTAGAAAGTAGGCGG+TGG - chr2.4:1957936-1957955 None:intergenic 55.0%
GGCGGTGGCATGTAGAAAGT+AGG - chr2.4:1957942-1957961 None:intergenic 55.0%
GTCGCCGTTCTCCGATATTG+AGG + chr2.4:1957890-1957909 MS.gene89034:CDS 55.0%
! GAGAACCATTGTGTTGGCGG+TGG - chr2.4:1957957-1957976 None:intergenic 55.0%
! GGTGCATCGGGTGCACTTTT+TGG + chr2.4:1959056-1959075 MS.gene89034:CDS 55.0%
! TAGAAAGTAGGCGGTGGTGG+CGG - chr2.4:1957930-1957949 None:intergenic 55.0%
GGACAGAAGGGCCAAGGAGA+GGG - chr2.4:1958127-1958146 None:intergenic 60.0%
GGACAGTGTTCCGTGACTCG+GGG + chr2.4:1959212-1959231 MS.gene89034:intron 60.0%
! AGAAAGTAGGCGGTGGTGGC+GGG - chr2.4:1957929-1957948 None:intergenic 60.0%
ACGTGCGGACAGAAGGGCCA+AGG - chr2.4:1958133-1958152 None:intergenic 65.0%
CGGACAGAAGGGCCAAGGAG+AGG - chr2.4:1958128-1958147 None:intergenic 65.0%
! GAAAGTAGGCGGTGGTGGCG+GGG - chr2.4:1957928-1957947 None:intergenic 65.0%
! GTTCCGTGACTCGGGGTGCA+GGG + chr2.4:1959219-1959238 MS.gene89034:intron 65.0%
! TGTTCCGTGACTCGGGGTGC+AGG + chr2.4:1959218-1959237 MS.gene89034:intron 65.0%
! TTCCGTGACTCGGGGTGCAG+GGG + chr2.4:1959220-1959239 MS.gene89034:intron 65.0%
!! AAAGTAGGCGGTGGTGGCGG+GGG - chr2.4:1957927-1957946 None:intergenic 65.0%
! TCCGTGACTCGGGGTGCAGG+GGG + chr2.4:1959221-1959240 MS.gene89034:intron 70.0%
CCCCCCTGCACCCCGAGTCA+CGG - chr2.4:1959225-1959244 None:intergenic 75.0%
! CCGTGACTCGGGGTGCAGGG+GGG + chr2.4:1959222-1959241 MS.gene89034:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 1957879 1960131 1957879 ID=MS.gene89034
chr2.4 mRNA 1957879 1960131 1957879 ID=MS.gene89034.t1;Parent=MS.gene89034
chr2.4 exon 1957879 1958150 1957879 ID=MS.gene89034.t1.exon1;Parent=MS.gene89034.t1
chr2.4 CDS 1957879 1958150 1957879 ID=cds.MS.gene89034.t1;Parent=MS.gene89034.t1
chr2.4 exon 1958684 1958847 1958684 ID=MS.gene89034.t1.exon2;Parent=MS.gene89034.t1
chr2.4 CDS 1958684 1958847 1958684 ID=cds.MS.gene89034.t1;Parent=MS.gene89034.t1
chr2.4 exon 1958958 1959136 1958958 ID=MS.gene89034.t1.exon3;Parent=MS.gene89034.t1
chr2.4 CDS 1958958 1959136 1958958 ID=cds.MS.gene89034.t1;Parent=MS.gene89034.t1
chr2.4 exon 1959493 1959749 1959493 ID=MS.gene89034.t1.exon4;Parent=MS.gene89034.t1
chr2.4 CDS 1959493 1959749 1959493 ID=cds.MS.gene89034.t1;Parent=MS.gene89034.t1
chr2.4 exon 1960053 1960131 1960053 ID=MS.gene89034.t1.exon5;Parent=MS.gene89034.t1
chr2.4 CDS 1960053 1960131 1960053 ID=cds.MS.gene89034.t1;Parent=MS.gene89034.t1
Gene Sequence

>MS.gene89034

ATGGGGAAAAAGTCGCCGTTCTCCGATATTGAGGCGGCTCGTTATCCCCCGCCACCACCGCCTACTTTCTACATGCCACCGCCAACACAATGGTTCTCATGGTTGGTGCCACTCATTTTTTTAGCCAATATTGCAATGTTCGTGTATAGCATGTATATCAATGATTGCCCTGGCTATTTGAATGAAGATGATTGCCTTTGGTATCAATATTTGGGAAGATTTTCTTTTCAACCTTTCAATGAAAACCCTCTCCTTGGCCCTTCTGTCCGCACGTGAGAATCTTCTCCCTCTCAATTTCTCTCTCACATAATCATCATCATCATTTTAAATATCATGGTATAATGTTCAACAATGTACCTGGCTTTCTCATTAAAATATCATGGTATATTGCATTTTCATATTGAAAAGTTAAGATTTTTGAAATGATGAACATTTAGAATTTTTGAATCATCAATTTGAATGATTATGTCAAATGCATGTCCTGGATTACTTCCATGCATGGGGTTTTAAAATGTGGTTTGCAACAGCAATTGCAGCTTAACAAAAAATTGAGGTCTCTGCAATGACGTCGCAGCTACAATATCAACTGAATCGACCGCGTTTTGCCGAAATATAAAAAATTGCGGTGCGCAACAGCAATTTAAAACTATGCTTCTATTGTCTTACTTAGATGGTATTCAACATAAGACATAAAATGAAAATGCCTACAATACATGCATGCATAGGAAGAAGAAAAAAACAGTGTTAAGTTTCATCCTCTTTTGTCAATTGGTTGTTTTAGGGTTATTGTGTTTACATTCTGCAGGTTGAGGGTATTAGGAGCTCTTGAAAAGGACCTTGTGGTGGGTGAAAATGAAGTATGGCGGTTCTTTACTTGCATGTTTCTTCATGCAGGAGTTATACATTTACTTGCCAATATGTTTAGTCTTTTATTCATAGGAGTTCGCCTTGAGAATGAATTTGGATTTTGTAAGCATATCTCAATTTCTATTGAGTTTTTTTTTTTTTCAACAAGTTGAAACTCTTTTCTATCTTTGTTTAGTTGATTTACTTATTATATTTATTCTTGGATTTGGCAGTGAAAATTGGAGTTCTATATTTGCTTTCTGGCTTTGGTGGAAGTTTGTTATCTATTTTGCATTTGGGAGATGCTAAAGCACCAAATACCGTATCAGTTGGTGCATCGGGTGCACTTTTTGGACTTCTGGGAGCCATGCTTAGTGAGCTTCTAACCAATTGGACTATCTATTTAAATAAGGTACTTTATGATGATATATTTTGTTGTCTTGAAAGAAAGAAAAAAAAAATACATAGGGTGGGTGTGTGCCAAAAAGGACAGTGTTCCGTGACTCGGGGTGCAGGGGGGAGAAACCCCTTACACAGTACAGTTCAAAGTTAATCTTGTAAATTGGACTGTAGCATTTAAGTACTGTAAATCATAAGCTTGATTATAAAGAGGCGTTGTAGCCTCTACGGTCAAAAACACAGAGACAATTTGGTCATATCAAGTGATCAAATATGTGTTTTCTCTACTTGCATCTTTTGTATCTTTTTACTTTTGAACCAAGGCTGTTGTTCTAACAGATAGATTTGAAACCATGTTTACTTCTACAGTGTGCAGCTCTAACAAGTCTACTTCTCATCATTGGCCTAAATTTGGCTGTTGGATTTATACCTCATGTGGACAATTCAGCTCATATAGGAGGATTCCTATCAGGATTTTTCCTTGGTTTTGTTATTTTAATGCGCCCTCAATTTGGTTATGTGAACAACAAATATATACCACCAGGATATGATGCAAAACGTAAATCCAAGTATAAAGGCTATCAGTATTTCTTCCTGGTCTTGTCAGTCATTACGCTACTTATCGGGTAAGTAATTCATGATTTTCTGAGTTTAATTTCGATACAGTGTTGTCAATGTAAACAAATTTACACCGCCAAACAAATCACTATCATTTCATGTGTGTGACTTTTTGAATACTTATGATTAAAGTCAAACGTTTTTAATAATTTGAGGACTTTGATTGATTGGCAATGTAAAAAGTATCAACAGTGACAATGTATATAAATTTAATCATTTCTTTTTTTATGTTCAATCATAATGATGGAATTTGAATTTAATCTGTATTGGTTAACCTCCTTTAACTTTTGATGTAATTGCTCATTTGACAGATATGCCTATGGCTTAGCTACATTGTACATAGGAGAATCAAATGATGTATTTTCCTTCTTAGCGAATACTGAAAGTTAA
Protein sequence

>MS.gene89034.t1

MGKKSPFSDIEAARYPPPPPPTFYMPPPTQWFSWLVPLIFLANIAMFVYSMYINDCPGYLNEDDCLWYQYLGRFSFQPFNENPLLGPSVRTLRVLGALEKDLVVGENEVWRFFTCMFLHAGVIHLLANMFSLLFIGVRLENEFGFLKIGVLYLLSGFGGSLLSILHLGDAKAPNTVSVGASGALFGLLGAMLSELLTNWTIYLNKCAALTSLLLIIGLNLAVGFIPHVDNSAHIGGFLSGFFLGFVILMRPQFGYVNNKYIPPGYDAKRKSKYKGYQYFFLVLSVITLLIGYAYGLATLYIGESNDVFSFLANTES