Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89267.t1 | XP_003623161.2 | 95.7 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 1.50E-57 | 231.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89267.t1 | G7L5L2 | 95.7 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 1.1e-57 | 231.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene89267 | 0.805707 | 1.17E-49 | -1.69E-46 |
| MS.gene051552 | MS.gene89267 | 0.849048 | 4.15E-60 | -1.69E-46 |
| MS.gene051907 | MS.gene89267 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene89267 | 0.807719 | 4.41E-50 | -1.69E-46 |
| MS.gene054884 | MS.gene89267 | 0.817439 | 3.30E-52 | -1.69E-46 |
| MS.gene055157 | MS.gene89267 | 0.830915 | 2.24E-55 | -1.69E-46 |
| MS.gene055158 | MS.gene89267 | 0.908419 | 1.74E-81 | -1.69E-46 |
| MS.gene055159 | MS.gene89267 | 0.976321 | 1.33E-141 | -1.69E-46 |
| MS.gene056688 | MS.gene89267 | 0.818993 | 1.47E-52 | -1.69E-46 |
| MS.gene058158 | MS.gene89267 | 0.802646 | 5.08E-49 | -1.69E-46 |
| MS.gene058418 | MS.gene89267 | 0.844583 | 6.91E-59 | -1.69E-46 |
| MS.gene05960 | MS.gene89267 | 0.810912 | 9.11E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89267.t1 | MTR_7g065160 | 95.726 | 117 | 5 | 0 | 1 | 117 | 1 | 117 | 7.73e-80 | 230 |
| MS.gene89267.t1 | MTR_7g065210 | 87.179 | 117 | 15 | 0 | 1 | 117 | 1 | 117 | 6.74e-73 | 213 |
| MS.gene89267.t1 | MTR_8g070040 | 45.455 | 121 | 61 | 3 | 1 | 117 | 1 | 120 | 3.87e-25 | 92.0 |
| MS.gene89267.t1 | MTR_8g070015 | 42.105 | 114 | 61 | 3 | 1 | 110 | 1 | 113 | 2.30e-19 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89267.t1 | AT5G05960 | 61.261 | 111 | 36 | 2 | 7 | 117 | 13 | 116 | 1.06e-42 | 136 |
| MS.gene89267.t1 | AT3G53980 | 64.211 | 95 | 33 | 1 | 23 | 117 | 21 | 114 | 2.86e-42 | 135 |
| MS.gene89267.t1 | AT3G53980 | 64.211 | 95 | 33 | 1 | 23 | 117 | 21 | 114 | 2.86e-42 | 135 |
| MS.gene89267.t1 | AT2G37870 | 49.107 | 112 | 51 | 4 | 10 | 117 | 6 | 115 | 5.31e-26 | 94.0 |
Find 21 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCAAGGTTGCAACGTTTA+GGG | 0.315042 | 7.1:-37140622 | None:intergenic |
| TCAGCAAGGTTGCAACGTTT+AGG | 0.352849 | 7.1:-37140623 | None:intergenic |
| GGTATTGCAGTTCTTGTTGC+AGG | 0.361788 | 7.1:+37140368 | MS.gene89267:CDS |
| AGCTTAAAAGCTTCATTGTC+TGG | 0.411064 | 7.1:-37140437 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.431901 | 7.1:+37140572 | MS.gene89267:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.1:-37140601 | None:intergenic |
| TGTGCTCAGGTTAAGAAACT+TGG | 0.492815 | 7.1:+37140515 | MS.gene89267:CDS |
| GTGAAATTGTTGTGCTTCGT+TGG | 0.500117 | 7.1:+37140347 | MS.gene89267:CDS |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.1:-37140602 | None:intergenic |
| GTCACAAAATTGTTGTGCTC+AGG | 0.514410 | 7.1:+37140502 | MS.gene89267:CDS |
| CGTCCACACTTGTAACCGAC+AGG | 0.520932 | 7.1:-37140650 | None:intergenic |
| CGCCCTGTCGGTTACAAGTG+TGG | 0.534054 | 7.1:+37140647 | MS.gene89267:CDS |
| GTTGAAAGTGCTGGTGAATG+TGG | 0.539242 | 7.1:+37140401 | MS.gene89267:CDS |
| AAAGTGCTGGTGAATGTGGA+AGG | 0.549386 | 7.1:+37140405 | MS.gene89267:CDS |
| AACCTTGCTGATCGCCCTGT+CGG | 0.551684 | 7.1:+37140635 | MS.gene89267:CDS |
| ATTCATAGAGTTGAAAGTGC+TGG | 0.554882 | 7.1:+37140392 | MS.gene89267:CDS |
| ATAACAGCACAAAGACAACT+TGG | 0.604458 | 7.1:-37140545 | None:intergenic |
| AGTGCTGGTGAATGTGGAAG+GGG | 0.630405 | 7.1:+37140407 | MS.gene89267:CDS |
| AACCGACAGGGCGATCAGCA+AGG | 0.631783 | 7.1:-37140637 | None:intergenic |
| AAGTGCTGGTGAATGTGGAA+GGG | 0.641454 | 7.1:+37140406 | MS.gene89267:CDS |
| GTCCACACTTGTAACCGACA+GGG | 0.709589 | 7.1:-37140649 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTATTTATCTTTTTTCT+TGG | + | chr7.1:37140924-37140943 | MS.gene89267:intron | 10.0% |
| !!! | TACTTATTTGACATTTTTTT+AGG | - | chr7.1:37140886-37140905 | None:intergenic | 15.0% |
| AGCTTAAAAGCTTCATTGTC+TGG | - | chr7.1:37140440-37140459 | None:intergenic | 35.0% | |
| ATAACAGCACAAAGACAACT+TGG | - | chr7.1:37140548-37140567 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | - | chr7.1:37140604-37140623 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.1:37140572-37140591 | MS.gene89267:CDS | 35.0% | |
| ! | ATTCATAGAGTTGAAAGTGC+TGG | + | chr7.1:37140392-37140411 | MS.gene89267:CDS | 35.0% |
| GGGATAGTAACAGCAATTTG+AGG | - | chr7.1:37140605-37140624 | None:intergenic | 40.0% | |
| GTCACAAAATTGTTGTGCTC+AGG | + | chr7.1:37140502-37140521 | MS.gene89267:CDS | 40.0% | |
| GTGAAATTGTTGTGCTTCGT+TGG | + | chr7.1:37140347-37140366 | MS.gene89267:CDS | 40.0% | |
| TGTGCTCAGGTTAAGAAACT+TGG | + | chr7.1:37140515-37140534 | MS.gene89267:CDS | 40.0% | |
| CAGCAAGGTTGCAACGTTTA+GGG | - | chr7.1:37140625-37140644 | None:intergenic | 45.0% | |
| GGTATTGCAGTTCTTGTTGC+AGG | + | chr7.1:37140368-37140387 | MS.gene89267:CDS | 45.0% | |
| TCAGCAAGGTTGCAACGTTT+AGG | - | chr7.1:37140626-37140645 | None:intergenic | 45.0% | |
| ! | TCTTTTGCTGCACTTGCACA+AGG | - | chr7.1:37140467-37140486 | None:intergenic | 45.0% |
| !! | AAAGTGCTGGTGAATGTGGA+AGG | + | chr7.1:37140405-37140424 | MS.gene89267:CDS | 45.0% |
| !! | AAGTGCTGGTGAATGTGGAA+GGG | + | chr7.1:37140406-37140425 | MS.gene89267:CDS | 45.0% |
| !! | GTTGAAAGTGCTGGTGAATG+TGG | + | chr7.1:37140401-37140420 | MS.gene89267:CDS | 45.0% |
| GTCCACACTTGTAACCGACA+GGG | - | chr7.1:37140652-37140671 | None:intergenic | 50.0% | |
| !! | AGTGCTGGTGAATGTGGAAG+GGG | + | chr7.1:37140407-37140426 | MS.gene89267:CDS | 50.0% |
| AACCTTGCTGATCGCCCTGT+CGG | + | chr7.1:37140635-37140654 | MS.gene89267:CDS | 55.0% | |
| CGTCCACACTTGTAACCGAC+AGG | - | chr7.1:37140653-37140672 | None:intergenic | 55.0% | |
| AACCGACAGGGCGATCAGCA+AGG | - | chr7.1:37140640-37140659 | None:intergenic | 60.0% | |
| ! | CGCCCTGTCGGTTACAAGTG+TGG | + | chr7.1:37140647-37140666 | MS.gene89267:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 37140335 | 37141011 | 37140335 | ID=MS.gene89267 |
| chr7.1 | mRNA | 37140335 | 37141011 | 37140335 | ID=MS.gene89267.t1;Parent=MS.gene89267 |
| chr7.1 | exon | 37140335 | 37140671 | 37140335 | ID=MS.gene89267.t1.exon1;Parent=MS.gene89267.t1 |
| chr7.1 | CDS | 37140335 | 37140671 | 37140335 | ID=cds.MS.gene89267.t1;Parent=MS.gene89267.t1 |
| chr7.1 | exon | 37140995 | 37141011 | 37140995 | ID=MS.gene89267.t1.exon2;Parent=MS.gene89267.t1 |
| chr7.1 | CDS | 37140995 | 37141011 | 37140995 | ID=cds.MS.gene89267.t1;Parent=MS.gene89267.t1 |
| Gene Sequence |
| Protein sequence |
