Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051907.t1 | XP_013450274.1 | 89.4 | 216 | 20 | 2 | 1 | 216 | 12 | 224 | 9.70E-109 | 402.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051907.t1 | Q9FG13 | 58.0 | 219 | 88 | 2 | 1 | 216 | 15 | 232 | 1.4e-68 | 260.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051907.t1 | A0A072U3J9 | 89.4 | 216 | 20 | 2 | 1 | 216 | 12 | 224 | 7.0e-109 | 402.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049265 | MS.gene051907 | 0.816299 | 5.94E-52 | -1.69E-46 |
| MS.gene050809 | MS.gene051907 | 0.81356 | 2.41E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene052702 | 0.818912 | 1.53E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene054290 | 0.829929 | 3.91E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene054884 | 0.803987 | 2.68E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene056678 | 0.813282 | 2.77E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene056856 | 0.829973 | 3.81E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene058158 | 0.865123 | 7.42E-65 | -1.69E-46 |
| MS.gene051907 | MS.gene059526 | 0.809703 | 1.66E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene065074 | 0.825068 | 5.73E-54 | -1.69E-46 |
| MS.gene051907 | MS.gene065110 | 0.818097 | 2.34E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene065426 | 0.806229 | 9.10E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene065680 | -0.800979 | 1.12E-48 | -1.69E-46 |
| MS.gene051907 | MS.gene066027 | 0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene069869 | 0.820451 | 6.81E-53 | -1.69E-46 |
| MS.gene051907 | MS.gene072609 | 0.807635 | 4.59E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene07837 | 0.812876 | 3.40E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene08658 | 0.802933 | 4.43E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene09072 | 0.806311 | 8.75E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene21274 | 0.80517 | 1.52E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene23057 | 0.948331 | 1.17E-106 | -1.69E-46 |
| MS.gene051907 | MS.gene23552 | 0.820209 | 7.74E-53 | -1.69E-46 |
| MS.gene051907 | MS.gene25982 | -0.817837 | 2.68E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene26624 | 0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene32771 | 0.816988 | 4.16E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene32895 | 0.859649 | 3.57E-63 | -1.69E-46 |
| MS.gene051907 | MS.gene34093 | 0.863675 | 2.10E-64 | -1.69E-46 |
| MS.gene051907 | MS.gene37747 | 0.801939 | 7.11E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene38375 | 0.818147 | 2.28E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene42017 | 0.850905 | 1.25E-60 | -1.69E-46 |
| MS.gene051907 | MS.gene42018 | 0.804032 | 2.62E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene42621 | 0.822172 | 2.73E-53 | -1.69E-46 |
| MS.gene051907 | MS.gene42687 | 0.813172 | 2.93E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene43392 | 0.802414 | 5.68E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene44712 | 0.810029 | 1.41E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene48183 | 0.804297 | 2.31E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene48807 | 0.801263 | 9.78E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene52979 | 0.803043 | 4.21E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene53436 | 0.801738 | 7.81E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene54347 | 0.805 | 1.65E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene55079 | 0.81395 | 1.97E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene55086 | 0.829263 | 5.68E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene58062 | -0.818306 | 2.10E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene58280 | -0.831099 | 2.02E-55 | -1.69E-46 |
| MS.gene051907 | MS.gene58282 | -0.809867 | 1.53E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene58786 | 0.809015 | 2.33E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene59400 | 0.833079 | 6.55E-56 | -1.69E-46 |
| MS.gene051907 | MS.gene59415 | 0.814803 | 1.28E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene59920 | 0.809726 | 1.64E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene60307 | 0.814226 | 1.72E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene60407 | 0.80261 | 5.17E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene64708 | 0.826049 | 3.36E-54 | -1.69E-46 |
| MS.gene051907 | MS.gene64872 | 0.814899 | 1.22E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene65821 | 0.804937 | 1.70E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene68849 | 0.81415 | 1.78E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene68946 | 0.823384 | 1.43E-53 | -1.69E-46 |
| MS.gene051907 | MS.gene70061 | 0.809895 | 1.51E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene74253 | 0.809379 | 1.95E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene74446 | 0.813932 | 1.99E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene76250 | 0.807045 | 6.12E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene76534 | 0.807429 | 5.08E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene77032 | 0.808679 | 2.75E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene77349 | 0.80332 | 3.69E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene78424 | 0.818198 | 2.22E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene79624 | 0.810995 | 8.74E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene80488 | 0.813202 | 2.88E-51 | -1.69E-46 |
| MS.gene051907 | MS.gene80979 | 0.804424 | 2.17E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene81954 | 0.815676 | 8.18E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene82245 | 0.807653 | 4.55E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene82858 | 0.805994 | 1.02E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene84195 | 0.821666 | 3.58E-53 | -1.69E-46 |
| MS.gene051907 | MS.gene85150 | 0.818376 | 2.02E-52 | -1.69E-46 |
| MS.gene051907 | MS.gene86563 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene87750 | 0.803063 | 4.17E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene89267 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene051907 | MS.gene89380 | 0.800169 | 1.63E-48 | -1.69E-46 |
| MS.gene051907 | MS.gene90899 | 0.805777 | 1.13E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene95132 | 0.821432 | 4.05E-53 | -1.69E-46 |
| MS.gene051907 | MS.gene96299 | 0.826514 | 2.60E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87832 | MS.gene051907 | PPI |
| MS.gene047803 | MS.gene051907 | PPI |
| MS.gene39627 | MS.gene051907 | PPI |
| MS.gene019395 | MS.gene051907 | PPI |
| MS.gene26527 | MS.gene051907 | PPI |
| MS.gene047804 | MS.gene051907 | PPI |
| MS.gene39628 | MS.gene051907 | PPI |
| MS.gene26526 | MS.gene051907 | PPI |
| MS.gene051907 | MS.gene053480 | PPI |
| MS.gene26528 | MS.gene051907 | PPI |
| MS.gene022960 | MS.gene051907 | PPI |
| MS.gene87834 | MS.gene051907 | PPI |
| MS.gene053470 | MS.gene051907 | PPI |
| MS.gene23639 | MS.gene051907 | PPI |
| MS.gene047802 | MS.gene051907 | PPI |
| MS.gene37504 | MS.gene051907 | PPI |
| MS.gene37503 | MS.gene051907 | PPI |
| MS.gene23692 | MS.gene051907 | PPI |
| MS.gene051696 | MS.gene051907 | PPI |
| MS.gene23641 | MS.gene051907 | PPI |
| MS.gene053479 | MS.gene051907 | PPI |
| MS.gene019397 | MS.gene051907 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051907.t1 | MTR_7g107040 | 88.426 | 216 | 22 | 2 | 1 | 216 | 12 | 224 | 1.02e-134 | 381 |
| MS.gene051907.t1 | MTR_4g050140 | 55.556 | 216 | 92 | 4 | 2 | 216 | 11 | 223 | 3.91e-76 | 232 |
| MS.gene051907.t1 | MTR_7g406880 | 37.321 | 209 | 115 | 6 | 2 | 198 | 27 | 231 | 9.30e-41 | 142 |
| MS.gene051907.t1 | MTR_1g102170 | 40.488 | 205 | 109 | 5 | 9 | 210 | 43 | 237 | 1.40e-40 | 141 |
| MS.gene051907.t1 | MTR_8g035520 | 39.286 | 196 | 114 | 5 | 4 | 197 | 21 | 213 | 2.16e-40 | 141 |
| MS.gene051907.t1 | MTR_1g012430 | 37.500 | 216 | 121 | 7 | 3 | 214 | 10 | 215 | 1.57e-39 | 138 |
| MS.gene051907.t1 | MTR_8g035540 | 40.000 | 205 | 104 | 6 | 4 | 197 | 9 | 205 | 1.66e-39 | 138 |
| MS.gene051907.t1 | MTR_1g102160 | 39.109 | 202 | 109 | 4 | 4 | 198 | 19 | 213 | 3.60e-39 | 137 |
| MS.gene051907.t1 | MTR_1g111280 | 37.864 | 206 | 110 | 6 | 2 | 198 | 32 | 228 | 8.02e-39 | 137 |
| MS.gene051907.t1 | MTR_6g464280 | 43.931 | 173 | 86 | 4 | 29 | 197 | 45 | 210 | 9.87e-38 | 134 |
| MS.gene051907.t1 | MTR_7g056207 | 36.058 | 208 | 126 | 4 | 3 | 207 | 47 | 250 | 2.91e-36 | 130 |
| MS.gene051907.t1 | MTR_6g464120 | 38.776 | 196 | 111 | 5 | 4 | 197 | 24 | 212 | 9.59e-36 | 132 |
| MS.gene051907.t1 | MTR_6g464510 | 37.113 | 194 | 111 | 4 | 8 | 197 | 27 | 213 | 2.93e-35 | 127 |
| MS.gene051907.t1 | MTR_6g464320 | 41.279 | 172 | 90 | 4 | 30 | 197 | 47 | 211 | 2.98e-35 | 127 |
| MS.gene051907.t1 | MTR_0007s0520 | 38.776 | 196 | 102 | 5 | 9 | 197 | 26 | 210 | 4.86e-35 | 127 |
| MS.gene051907.t1 | MTR_3g012440 | 35.065 | 231 | 124 | 9 | 2 | 212 | 13 | 237 | 3.43e-34 | 125 |
| MS.gene051907.t1 | MTR_3g101420 | 33.333 | 222 | 127 | 7 | 3 | 217 | 14 | 221 | 1.09e-32 | 120 |
| MS.gene051907.t1 | MTR_4g086510 | 33.645 | 214 | 133 | 5 | 3 | 213 | 15 | 222 | 4.45e-31 | 116 |
| MS.gene051907.t1 | MTR_5g005900 | 35.052 | 194 | 117 | 5 | 3 | 190 | 16 | 206 | 6.04e-31 | 116 |
| MS.gene051907.t1 | MTR_1g105020 | 36.765 | 204 | 119 | 8 | 3 | 199 | 15 | 215 | 6.46e-30 | 113 |
| MS.gene051907.t1 | MTR_3g450680 | 32.287 | 223 | 137 | 7 | 3 | 218 | 23 | 238 | 8.63e-30 | 113 |
| MS.gene051907.t1 | MTR_4g086480 | 33.333 | 195 | 125 | 4 | 4 | 197 | 20 | 210 | 1.23e-29 | 112 |
| MS.gene051907.t1 | MTR_8g040860 | 33.511 | 188 | 119 | 5 | 4 | 188 | 24 | 208 | 7.28e-28 | 108 |
| MS.gene051907.t1 | MTR_4g086470 | 32.394 | 213 | 139 | 4 | 3 | 214 | 18 | 226 | 1.25e-27 | 107 |
| MS.gene051907.t1 | MTR_6g065370 | 33.146 | 178 | 101 | 3 | 28 | 197 | 55 | 222 | 1.57e-27 | 107 |
| MS.gene051907.t1 | MTR_4g086540 | 34.031 | 191 | 113 | 6 | 7 | 195 | 29 | 208 | 1.96e-27 | 106 |
| MS.gene051907.t1 | MTR_6g065400 | 33.708 | 178 | 100 | 3 | 28 | 197 | 55 | 222 | 2.10e-27 | 107 |
| MS.gene051907.t1 | MTR_5g005920 | 34.896 | 192 | 118 | 5 | 4 | 190 | 21 | 210 | 3.49e-27 | 106 |
| MS.gene051907.t1 | MTR_6g065340 | 32.432 | 185 | 104 | 4 | 28 | 201 | 66 | 240 | 4.14e-27 | 106 |
| MS.gene051907.t1 | MTR_6g065110 | 32.584 | 178 | 102 | 3 | 28 | 197 | 56 | 223 | 6.54e-27 | 105 |
| MS.gene051907.t1 | MTR_8g035500 | 31.507 | 219 | 131 | 7 | 4 | 211 | 12 | 222 | 1.00e-26 | 104 |
| MS.gene051907.t1 | MTR_6g065270 | 34.066 | 182 | 98 | 3 | 28 | 197 | 53 | 224 | 1.22e-26 | 105 |
| MS.gene051907.t1 | MTR_6g065120 | 32.796 | 186 | 103 | 4 | 28 | 201 | 56 | 231 | 1.33e-26 | 105 |
| MS.gene051907.t1 | MTR_6g065430 | 35.088 | 171 | 100 | 3 | 28 | 197 | 56 | 216 | 1.26e-25 | 102 |
| MS.gene051907.t1 | MTR_1g089310 | 39.568 | 139 | 71 | 2 | 60 | 198 | 121 | 246 | 6.24e-25 | 100 |
| MS.gene051907.t1 | MTR_1g089310 | 39.568 | 139 | 71 | 2 | 60 | 198 | 105 | 230 | 7.96e-25 | 100 |
| MS.gene051907.t1 | MTR_1g082210 | 34.463 | 177 | 91 | 5 | 34 | 198 | 67 | 230 | 1.76e-24 | 99.4 |
| MS.gene051907.t1 | MTR_7g093950 | 37.681 | 138 | 76 | 2 | 60 | 197 | 106 | 233 | 2.19e-23 | 96.3 |
| MS.gene051907.t1 | MTR_5g005850 | 31.731 | 208 | 123 | 7 | 1 | 197 | 18 | 217 | 2.55e-23 | 95.9 |
| MS.gene051907.t1 | MTR_7g051020 | 34.759 | 187 | 119 | 3 | 3 | 188 | 19 | 203 | 2.82e-22 | 93.2 |
| MS.gene051907.t1 | MTR_1g104930 | 34.759 | 187 | 116 | 4 | 3 | 188 | 19 | 200 | 8.04e-21 | 89.0 |
| MS.gene051907.t1 | MTR_1g104933 | 35.106 | 188 | 114 | 6 | 3 | 188 | 17 | 198 | 1.89e-19 | 85.1 |
| MS.gene051907.t1 | MTR_6g022710 | 32.402 | 179 | 91 | 5 | 48 | 197 | 146 | 323 | 1.70e-17 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051907.t1 | AT5G06570 | 57.991 | 219 | 88 | 2 | 1 | 216 | 15 | 232 | 2.10e-75 | 231 |
| MS.gene051907.t1 | AT5G06570 | 57.991 | 219 | 88 | 2 | 1 | 216 | 15 | 232 | 2.10e-75 | 231 |
| MS.gene051907.t1 | AT5G06570 | 57.991 | 219 | 88 | 2 | 1 | 216 | 30 | 247 | 2.47e-75 | 231 |
| MS.gene051907.t1 | AT5G06570 | 57.991 | 219 | 88 | 2 | 1 | 216 | 30 | 247 | 2.47e-75 | 231 |
| MS.gene051907.t1 | AT5G16080 | 41.294 | 201 | 109 | 5 | 2 | 198 | 34 | 229 | 3.06e-43 | 149 |
| MS.gene051907.t1 | AT5G62180 | 41.624 | 197 | 100 | 5 | 9 | 197 | 21 | 210 | 4.65e-40 | 140 |
| MS.gene051907.t1 | AT2G45600 | 40.102 | 197 | 106 | 5 | 4 | 197 | 16 | 203 | 1.48e-39 | 139 |
| MS.gene051907.t1 | AT1G68620 | 38.889 | 198 | 111 | 4 | 2 | 197 | 29 | 218 | 9.72e-39 | 137 |
| MS.gene051907.t1 | AT2G45610 | 41.071 | 168 | 93 | 4 | 33 | 197 | 49 | 213 | 6.71e-36 | 129 |
| MS.gene051907.t1 | AT5G23530 | 38.462 | 195 | 105 | 6 | 9 | 198 | 35 | 219 | 2.45e-33 | 122 |
| MS.gene051907.t1 | AT1G19190 | 33.511 | 188 | 120 | 4 | 4 | 188 | 13 | 198 | 3.88e-31 | 116 |
| MS.gene051907.t1 | AT1G49640 | 33.161 | 193 | 115 | 7 | 3 | 191 | 13 | 195 | 4.85e-30 | 113 |
| MS.gene051907.t1 | AT1G47480 | 31.414 | 191 | 119 | 5 | 1 | 190 | 13 | 192 | 3.46e-28 | 108 |
| MS.gene051907.t1 | AT1G49650 | 34.375 | 192 | 118 | 5 | 3 | 191 | 68 | 254 | 5.31e-27 | 106 |
| MS.gene051907.t1 | AT3G48690 | 31.280 | 211 | 138 | 6 | 3 | 209 | 12 | 219 | 1.18e-26 | 104 |
| MS.gene051907.t1 | AT3G48700 | 31.373 | 204 | 130 | 5 | 3 | 198 | 12 | 213 | 1.99e-26 | 104 |
| MS.gene051907.t1 | AT1G49660 | 32.812 | 192 | 120 | 5 | 3 | 190 | 12 | 198 | 4.15e-25 | 100 |
| MS.gene051907.t1 | AT2G03550 | 32.632 | 190 | 115 | 6 | 3 | 188 | 12 | 192 | 7.21e-25 | 99.8 |
| MS.gene051907.t1 | AT5G27320 | 35.519 | 183 | 92 | 5 | 28 | 198 | 61 | 229 | 1.02e-24 | 100 |
| MS.gene051907.t1 | AT3G63010 | 34.286 | 175 | 88 | 3 | 38 | 198 | 70 | 231 | 1.84e-24 | 99.4 |
| MS.gene051907.t1 | AT3G05120 | 34.270 | 178 | 91 | 4 | 34 | 198 | 67 | 231 | 6.47e-24 | 97.8 |
| MS.gene051907.t1 | AT5G14310 | 32.044 | 181 | 90 | 4 | 48 | 197 | 140 | 318 | 1.13e-17 | 81.3 |
| MS.gene051907.t1 | AT3G27320 | 30.601 | 183 | 95 | 3 | 46 | 197 | 120 | 301 | 1.72e-17 | 80.9 |
| MS.gene051907.t1 | AT3G27320 | 30.601 | 183 | 95 | 3 | 46 | 197 | 152 | 333 | 1.95e-17 | 80.9 |
Find 63 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATACAACAATTGTGAATA+TGG | 0.214269 | 7.1:-6114027 | None:intergenic |
| ACATAACCTCTTATTCTTAC+AGG | 0.242199 | 7.1:-6114313 | None:intergenic |
| CTTTGACAGTGTGTTTATTA+TGG | 0.244190 | 7.1:+6114219 | MS.gene051907:CDS |
| GAAGTTGTTAGAACGGTAAA+TGG | 0.252525 | 7.1:-6113822 | None:intergenic |
| CCAGTGATTGTGTTCCTTCA+TGG | 0.273519 | 7.1:+6113971 | MS.gene051907:CDS |
| TTTATTATGGGTGACTCTTC+TGG | 0.275927 | 7.1:+6114232 | MS.gene051907:CDS |
| TCAAGATTCAATGTTTGTTC+TGG | 0.294664 | 7.1:-6114391 | None:intergenic |
| TGGAGGACCTTCCTCAGATT+TGG | 0.297434 | 7.1:-6114371 | None:intergenic |
| CACCAGCTCGCGGTTCGGTT+TGG | 0.326565 | 7.1:+6114268 | MS.gene051907:CDS |
| TTCTTGTTGTTGATGTTGTT+TGG | 0.341111 | 7.1:-6113938 | None:intergenic |
| GGTGAGGTGGCTTCAAAGGC+AGG | 0.367234 | 7.1:+6114147 | MS.gene051907:CDS |
| TTTGACAGTGTGTTTATTAT+GGG | 0.368917 | 7.1:+6114220 | MS.gene051907:CDS |
| ACTGAGTTGTCTTCTATTGT+GGG | 0.382901 | 7.1:-6113857 | None:intergenic |
| TTTGTTTCGGCTCCCGTACA+TGG | 0.389708 | 7.1:+6114002 | MS.gene051907:CDS |
| CAGGGGTTGAGCCATGGTTA+TGG | 0.420795 | 7.1:+6114166 | MS.gene051907:CDS |
| CATACAACAATTGTGAATAT+GGG | 0.426547 | 7.1:-6114026 | None:intergenic |
| ATTGAACCTGTAAGAATAAG+AGG | 0.436336 | 7.1:+6114307 | MS.gene051907:CDS |
| TACTGAGTTGTCTTCTATTG+TGG | 0.438893 | 7.1:-6113858 | None:intergenic |
| GTGAATATGGGGCCATGTAC+GGG | 0.442379 | 7.1:-6114014 | None:intergenic |
| GTCGAAAAGACAGTCTTTGA+AGG | 0.443728 | 7.1:-6113882 | None:intergenic |
| TGTGAATATGGGGCCATGTA+CGG | 0.460692 | 7.1:-6114015 | None:intergenic |
| TTGCTCACCAGCTCGCGGTT+CGG | 0.462960 | 7.1:+6114263 | MS.gene051907:CDS |
| GAAATTTGAAGTTGTTAGAA+CGG | 0.477889 | 7.1:-6113829 | None:intergenic |
| TCTGGTGCAAGCCGGTAGTC+CGG | 0.481494 | 7.1:-6114085 | None:intergenic |
| GCTCGCGGTTCGGTTTGGAT+TGG | 0.481933 | 7.1:+6114273 | MS.gene051907:CDS |
| AACCGTCGCTATAGAGTTGA+AGG | 0.495505 | 7.1:-6113799 | None:intergenic |
| GCAGCTGGAAGACGGTGCTC+TGG | 0.503385 | 7.1:-6114103 | None:intergenic |
| AGATTCAATGTTTGTTCTGG+AGG | 0.507064 | 7.1:-6114388 | None:intergenic |
| AAGCGGTGAGGTGGCTTCAA+AGG | 0.511531 | 7.1:+6114143 | MS.gene051907:CDS |
| TGGTGGTTGCACCGGACTAC+CGG | 0.511979 | 7.1:+6114074 | MS.gene051907:CDS |
| TCAAGCACTGGTGGTTGCAC+CGG | 0.513886 | 7.1:+6114066 | MS.gene051907:CDS |
| CCATGAAGGAACACAATCAC+TGG | 0.524194 | 7.1:-6113971 | None:intergenic |
| GTGAGGTGGCTTCAAAGGCA+GGG | 0.524997 | 7.1:+6114148 | MS.gene051907:CDS |
| ACAGCATCATCAATTGCAGC+TGG | 0.532717 | 7.1:-6114118 | None:intergenic |
| ATTGTGTTCCTTCATGGTGG+AGG | 0.533059 | 7.1:+6113977 | MS.gene051907:CDS |
| GACGGTGCTCTGGTGCAAGC+CGG | 0.547439 | 7.1:-6114093 | None:intergenic |
| TTCCTTCAACTCTATAGCGA+CGG | 0.551743 | 7.1:+6113797 | MS.gene051907:CDS |
| CGCTGGACTTCAAGCACTGG+TGG | 0.553404 | 7.1:+6114057 | MS.gene051907:CDS |
| TGAATCTTGACTTACTAGAC+AGG | 0.555572 | 7.1:+6114404 | MS.gene051907:CDS |
| CAAAGGCAGGGGTTGAGCCA+TGG | 0.559944 | 7.1:+6114160 | MS.gene051907:CDS |
| ATCCAAACCGAACCGCGAGC+TGG | 0.561333 | 7.1:-6114270 | None:intergenic |
| TGAGGTGGCTTCAAAGGCAG+GGG | 0.564606 | 7.1:+6114149 | MS.gene051907:CDS |
| CATCAATTGCAGCTGGAAGA+CGG | 0.570423 | 7.1:-6114111 | None:intergenic |
| AACAAAAGCCTCCACCATGA+AGG | 0.573822 | 7.1:-6113985 | None:intergenic |
| TTATGGGTGACTCTTCTGGC+GGG | 0.581327 | 7.1:+6114236 | MS.gene051907:CDS |
| TGATGTTGTTTGGTTTGTAG+AGG | 0.582952 | 7.1:-6113928 | None:intergenic |
| AGCCGCTGGACTTCAAGCAC+TGG | 0.585205 | 7.1:+6114054 | MS.gene051907:CDS |
| ACTGTCAAAGTCAACATCAC+TGG | 0.588550 | 7.1:-6114206 | None:intergenic |
| TGTTGTATGAGTCTAGCCGC+TGG | 0.594495 | 7.1:+6114040 | MS.gene051907:CDS |
| ATTATGGGTGACTCTTCTGG+CGG | 0.606698 | 7.1:+6114235 | MS.gene051907:CDS |
| TCGGTTTGGATTGGATCGAA+CGG | 0.608973 | 7.1:+6114282 | MS.gene051907:CDS |
| ATACAACAATTGTGAATATG+GGG | 0.617658 | 7.1:-6114025 | None:intergenic |
| ATGCTGTCGAAGCGGTGAGG+TGG | 0.618657 | 7.1:+6114134 | MS.gene051907:CDS |
| CACCAGTGCTTGAAGTCCAG+CGG | 0.635664 | 7.1:-6114056 | None:intergenic |
| ACATCACTGGTCGTCAACCA+TGG | 0.636649 | 7.1:-6114193 | None:intergenic |
| GCCATGGTTATGGAGAGCCA+TGG | 0.645828 | 7.1:+6114176 | MS.gene051907:CDS |
| GTGATTGTGTTCCTTCATGG+TGG | 0.647455 | 7.1:+6113974 | MS.gene051907:CDS |
| GGAAGTACGGACCAAATCTG+AGG | 0.652219 | 7.1:+6114360 | MS.gene051907:CDS |
| GTACGGACCAAATCTGAGGA+AGG | 0.656456 | 7.1:+6114364 | MS.gene051907:CDS |
| GAATATTGCTCACCAGCTCG+CGG | 0.656723 | 7.1:+6114258 | MS.gene051907:CDS |
| ACCATGGCTCTCCATAACCA+TGG | 0.673171 | 7.1:-6114177 | None:intergenic |
| AATTGATGATGCTGTCGAAG+CGG | 0.713811 | 7.1:+6114126 | MS.gene051907:CDS |
| ATGATGCTGTCGAAGCGGTG+AGG | 0.739300 | 7.1:+6114131 | MS.gene051907:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAATTTCTCATCTAATTTT+CGG | + | chr7.1:6114518-6114537 | MS.gene051907:intron | 20.0% |
| ! | ATACAACAATTGTGAATATG+GGG | - | chr7.1:6114028-6114047 | None:intergenic | 25.0% |
| ! | CATACAACAATTGTGAATAT+GGG | - | chr7.1:6114029-6114048 | None:intergenic | 25.0% |
| ! | TCATACAACAATTGTGAATA+TGG | - | chr7.1:6114030-6114049 | None:intergenic | 25.0% |
| ! | TTTGACAGTGTGTTTATTAT+GGG | + | chr7.1:6114220-6114239 | MS.gene051907:CDS | 25.0% |
| !! | AATAAGAGGTTATGTTTTGT+TGG | + | chr7.1:6114321-6114340 | MS.gene051907:CDS | 25.0% |
| !! | GAAATTTGAAGTTGTTAGAA+CGG | - | chr7.1:6113832-6113851 | None:intergenic | 25.0% |
| ACATAACCTCTTATTCTTAC+AGG | - | chr7.1:6114316-6114335 | None:intergenic | 30.0% | |
| ATTGAACCTGTAAGAATAAG+AGG | + | chr7.1:6114307-6114326 | MS.gene051907:CDS | 30.0% | |
| CTTTGACAGTGTGTTTATTA+TGG | + | chr7.1:6114219-6114238 | MS.gene051907:CDS | 30.0% | |
| TCAAGATTCAATGTTTGTTC+TGG | - | chr7.1:6114394-6114413 | None:intergenic | 30.0% | |
| !! | TTCTTGTTGTTGATGTTGTT+TGG | - | chr7.1:6113941-6113960 | None:intergenic | 30.0% |
| ACTGAGTTGTCTTCTATTGT+GGG | - | chr7.1:6113860-6113879 | None:intergenic | 35.0% | |
| AGATTCAATGTTTGTTCTGG+AGG | - | chr7.1:6114391-6114410 | None:intergenic | 35.0% | |
| GAAGTTGTTAGAACGGTAAA+TGG | - | chr7.1:6113825-6113844 | None:intergenic | 35.0% | |
| TACTGAGTTGTCTTCTATTG+TGG | - | chr7.1:6113861-6113880 | None:intergenic | 35.0% | |
| TGAATCTTGACTTACTAGAC+AGG | + | chr7.1:6114404-6114423 | MS.gene051907:CDS | 35.0% | |
| TTTATTATGGGTGACTCTTC+TGG | + | chr7.1:6114232-6114251 | MS.gene051907:CDS | 35.0% | |
| !! | TGATGTTGTTTGGTTTGTAG+AGG | - | chr7.1:6113931-6113950 | None:intergenic | 35.0% |
| ACTGTCAAAGTCAACATCAC+TGG | - | chr7.1:6114209-6114228 | None:intergenic | 40.0% | |
| GTCGAAAAGACAGTCTTTGA+AGG | - | chr7.1:6113885-6113904 | None:intergenic | 40.0% | |
| TTCCTTCAACTCTATAGCGA+CGG | + | chr7.1:6113797-6113816 | MS.gene051907:CDS | 40.0% | |
| !! | AATTGATGATGCTGTCGAAG+CGG | + | chr7.1:6114126-6114145 | MS.gene051907:CDS | 40.0% |
| AACAAAAGCCTCCACCATGA+AGG | - | chr7.1:6113988-6114007 | None:intergenic | 45.0% | |
| AACCGTCGCTATAGAGTTGA+AGG | - | chr7.1:6113802-6113821 | None:intergenic | 45.0% | |
| ACAGCATCATCAATTGCAGC+TGG | - | chr7.1:6114121-6114140 | None:intergenic | 45.0% | |
| ATTATGGGTGACTCTTCTGG+CGG | + | chr7.1:6114235-6114254 | MS.gene051907:CDS | 45.0% | |
| ATTGTGTTCCTTCATGGTGG+AGG | + | chr7.1:6113977-6113996 | MS.gene051907:CDS | 45.0% | |
| CATCAATTGCAGCTGGAAGA+CGG | - | chr7.1:6114114-6114133 | None:intergenic | 45.0% | |
| CCAGTGATTGTGTTCCTTCA+TGG | + | chr7.1:6113971-6113990 | MS.gene051907:CDS | 45.0% | |
| CCATGAAGGAACACAATCAC+TGG | - | chr7.1:6113974-6113993 | None:intergenic | 45.0% | |
| CTTGACTTACTAGACAGGTG+AGG | + | chr7.1:6114409-6114428 | MS.gene051907:intron | 45.0% | |
| GTGATTGTGTTCCTTCATGG+TGG | + | chr7.1:6113974-6113993 | MS.gene051907:CDS | 45.0% | |
| TGTGAATATGGGGCCATGTA+CGG | - | chr7.1:6114018-6114037 | None:intergenic | 45.0% | |
| !! | CATGGTGGAGGCTTTTGTTT+CGG | + | chr7.1:6113989-6114008 | MS.gene051907:CDS | 45.0% |
| !! | TCGGTTTGGATTGGATCGAA+CGG | + | chr7.1:6114282-6114301 | MS.gene051907:CDS | 45.0% |
| !!! | GTTTTGTTGGCGCCGTTTTT+TGG | + | chr7.1:6114334-6114353 | MS.gene051907:CDS | 45.0% |
| !!! | AAATTAGAATAAATTATTTT+AGG | - | chr7.1:6114466-6114485 | None:intergenic | 5.0% |
| !!! | AATTAGAATAAATTATTTTA+GGG | - | chr7.1:6114465-6114484 | None:intergenic | 5.0% |
| ACATCACTGGTCGTCAACCA+TGG | - | chr7.1:6114196-6114215 | None:intergenic | 50.0% | |
| ACCATGGCTCTCCATAACCA+TGG | - | chr7.1:6114180-6114199 | None:intergenic | 50.0% | |
| CGTACTTCCCCGCCAAAAAA+CGG | - | chr7.1:6114349-6114368 | None:intergenic | 50.0% | |
| GAATATTGCTCACCAGCTCG+CGG | + | chr7.1:6114258-6114277 | MS.gene051907:CDS | 50.0% | |
| GTACGGACCAAATCTGAGGA+AGG | + | chr7.1:6114364-6114383 | MS.gene051907:CDS | 50.0% | |
| GTGAATATGGGGCCATGTAC+GGG | - | chr7.1:6114017-6114036 | None:intergenic | 50.0% | |
| TGGAGGACCTTCCTCAGATT+TGG | - | chr7.1:6114374-6114393 | None:intergenic | 50.0% | |
| TGTTGTATGAGTCTAGCCGC+TGG | + | chr7.1:6114040-6114059 | MS.gene051907:CDS | 50.0% | |
| TTATGGGTGACTCTTCTGGC+GGG | + | chr7.1:6114236-6114255 | MS.gene051907:CDS | 50.0% | |
| TTTGTTTCGGCTCCCGTACA+TGG | + | chr7.1:6114002-6114021 | MS.gene051907:CDS | 50.0% | |
| ! | GGAAGTACGGACCAAATCTG+AGG | + | chr7.1:6114360-6114379 | MS.gene051907:CDS | 50.0% |
| !!! | CGTTTTTTGGCGGGGAAGTA+CGG | + | chr7.1:6114347-6114366 | MS.gene051907:CDS | 50.0% |
| !!! | TTGTTGGCGCCGTTTTTTGG+CGG | + | chr7.1:6114337-6114356 | MS.gene051907:CDS | 50.0% |
| AAGCGGTGAGGTGGCTTCAA+AGG | + | chr7.1:6114143-6114162 | MS.gene051907:CDS | 55.0% | |
| ATGATGCTGTCGAAGCGGTG+AGG | + | chr7.1:6114131-6114150 | MS.gene051907:CDS | 55.0% | |
| CACCAGTGCTTGAAGTCCAG+CGG | - | chr7.1:6114059-6114078 | None:intergenic | 55.0% | |
| CAGGGGTTGAGCCATGGTTA+TGG | + | chr7.1:6114166-6114185 | MS.gene051907:CDS | 55.0% | |
| GCCATGGTTATGGAGAGCCA+TGG | + | chr7.1:6114176-6114195 | MS.gene051907:CDS | 55.0% | |
| GTGAGGTGGCTTCAAAGGCA+GGG | + | chr7.1:6114148-6114167 | MS.gene051907:CDS | 55.0% | |
| TGAGGTGGCTTCAAAGGCAG+GGG | + | chr7.1:6114149-6114168 | MS.gene051907:CDS | 55.0% | |
| !! | TCAAGCACTGGTGGTTGCAC+CGG | + | chr7.1:6114066-6114085 | MS.gene051907:CDS | 55.0% |
| !!! | TGTTGGCGCCGTTTTTTGGC+GGG | + | chr7.1:6114338-6114357 | MS.gene051907:CDS | 55.0% |
| AGCCGCTGGACTTCAAGCAC+TGG | + | chr7.1:6114054-6114073 | MS.gene051907:CDS | 60.0% | |
| ATCCAAACCGAACCGCGAGC+TGG | - | chr7.1:6114273-6114292 | None:intergenic | 60.0% | |
| ATGCTGTCGAAGCGGTGAGG+TGG | + | chr7.1:6114134-6114153 | MS.gene051907:CDS | 60.0% | |
| CAAAGGCAGGGGTTGAGCCA+TGG | + | chr7.1:6114160-6114179 | MS.gene051907:CDS | 60.0% | |
| GGTGAGGTGGCTTCAAAGGC+AGG | + | chr7.1:6114147-6114166 | MS.gene051907:CDS | 60.0% | |
| TCTGGTGCAAGCCGGTAGTC+CGG | - | chr7.1:6114088-6114107 | None:intergenic | 60.0% | |
| TGGTGGTTGCACCGGACTAC+CGG | + | chr7.1:6114074-6114093 | MS.gene051907:CDS | 60.0% | |
| TTGCTCACCAGCTCGCGGTT+CGG | + | chr7.1:6114263-6114282 | MS.gene051907:CDS | 60.0% | |
| !! | CGCTGGACTTCAAGCACTGG+TGG | + | chr7.1:6114057-6114076 | MS.gene051907:CDS | 60.0% |
| !! | GCTCGCGGTTCGGTTTGGAT+TGG | + | chr7.1:6114273-6114292 | MS.gene051907:CDS | 60.0% |
| !!! | GTTGGCGCCGTTTTTTGGCG+GGG | + | chr7.1:6114339-6114358 | MS.gene051907:CDS | 60.0% |
| GACGGTGCTCTGGTGCAAGC+CGG | - | chr7.1:6114096-6114115 | None:intergenic | 65.0% | |
| GCAGCTGGAAGACGGTGCTC+TGG | - | chr7.1:6114106-6114125 | None:intergenic | 65.0% | |
| !! | CACCAGCTCGCGGTTCGGTT+TGG | + | chr7.1:6114268-6114287 | MS.gene051907:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 6113791 | 6114556 | 6113791 | ID=MS.gene051907 |
| chr7.1 | mRNA | 6113791 | 6114556 | 6113791 | ID=MS.gene051907.t1;Parent=MS.gene051907 |
| chr7.1 | exon | 6113791 | 6114425 | 6113791 | ID=MS.gene051907.t1.exon1;Parent=MS.gene051907.t1 |
| chr7.1 | CDS | 6113791 | 6114425 | 6113791 | ID=cds.MS.gene051907.t1;Parent=MS.gene051907.t1 |
| chr7.1 | exon | 6114520 | 6114556 | 6114520 | ID=MS.gene051907.t1.exon2;Parent=MS.gene051907.t1 |
| chr7.1 | CDS | 6114520 | 6114556 | 6114520 | ID=cds.MS.gene051907.t1;Parent=MS.gene051907.t1 |
| Gene Sequence |
| Protein sequence |
