Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89327.t1 | ACJ84726.1 | 95.7 | 69 | 3 | 0 | 1 | 69 | 31 | 99 | 1.90E-31 | 144.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89327.t1 | Q9ZW04 | 63.2 | 68 | 25 | 0 | 2 | 69 | 33 | 100 | 1.9e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89327.t1 | B7FIZ7 | 95.7 | 69 | 3 | 0 | 1 | 69 | 31 | 99 | 1.4e-31 | 144.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051789 | MS.gene89327 | 0.80345 | 3.47E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene89327 | 0.85378 | 1.91E-61 | -1.69E-46 |
| MS.gene054596 | MS.gene89327 | 0.800682 | 1.29E-48 | -1.69E-46 |
| MS.gene057750 | MS.gene89327 | 0.803303 | 3.72E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene89327 | 0.802865 | 4.58E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene89327 | 0.800504 | 1.40E-48 | -1.69E-46 |
| MS.gene059006 | MS.gene89327 | 0.82083 | 5.57E-53 | -1.69E-46 |
| MS.gene059124 | MS.gene89327 | 0.838944 | 2.13E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGAAGAGTTGGCAGAATT+TGG | 0.288532 | 7.3:-42049159 | MS.gene89327:CDS |
| AGATGATATTAACAAATGTT+TGG | 0.374991 | 7.3:-42049106 | MS.gene89327:CDS |
| TTAACAAATGTTTGGAAGAG+TGG | 0.416511 | 7.3:-42049098 | MS.gene89327:CDS |
| GTTGATGGAAACTGTTGCTT+CGG | 0.441499 | 7.3:-42049010 | MS.gene89327:CDS |
| ATCAATCAGGTATGCAATTG+TGG | 0.492239 | 7.3:-42049178 | None:intergenic |
| ACTGTTGCTTCGGTCTTCGA+TGG | 0.504556 | 7.3:-42049000 | MS.gene89327:CDS |
| GTATGCAATTGTGGAAGAGT+TGG | 0.535529 | 7.3:-42049169 | MS.gene89327:CDS |
| TTGGAAGAGTGGAAAGGCAA+AGG | 0.565426 | 7.3:-42049087 | MS.gene89327:CDS |
| AAATGTTTGGAAGAGTGGAA+AGG | 0.633316 | 7.3:-42049093 | MS.gene89327:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATGATATTAACAAATGTT+TGG | - | chr7.3:42049047-42049066 | MS.gene89327:CDS | 20.0% |
| TTAACAAATGTTTGGAAGAG+TGG | - | chr7.3:42049055-42049074 | MS.gene89327:CDS | 30.0% | |
| ! | TTTCCATCAACTTTTTACGT+TGG | + | chr7.3:42049134-42049153 | None:intergenic | 30.0% |
| AAATGTTTGGAAGAGTGGAA+AGG | - | chr7.3:42049060-42049079 | MS.gene89327:CDS | 35.0% | |
| TGACCAACGTAAAAAGTTGA+TGG | - | chr7.3:42049128-42049147 | MS.gene89327:CDS | 35.0% | |
| GTATGCAATTGTGGAAGAGT+TGG | - | chr7.3:42048984-42049003 | MS.gene89327:CDS | 40.0% | |
| ! | GCAAAGGATTTTGTGTGACA+GGG | - | chr7.3:42049082-42049101 | MS.gene89327:CDS | 40.0% |
| ! | GTTGATGGAAACTGTTGCTT+CGG | - | chr7.3:42049143-42049162 | MS.gene89327:CDS | 40.0% |
| GTGGAAGAGTTGGCAGAATT+TGG | - | chr7.3:42048994-42049013 | MS.gene89327:CDS | 45.0% | |
| TTGGAAGAGTGGAAAGGCAA+AGG | - | chr7.3:42049066-42049085 | MS.gene89327:CDS | 45.0% | |
| ! | GGCAAAGGATTTTGTGTGAC+AGG | - | chr7.3:42049081-42049100 | MS.gene89327:CDS | 45.0% |
| ! | ACTGTTGCTTCGGTCTTCGA+TGG | - | chr7.3:42049153-42049172 | MS.gene89327:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 42048984 | 42049191 | 42048984 | ID=MS.gene89327 |
| chr7.3 | mRNA | 42048984 | 42049191 | 42048984 | ID=MS.gene89327.t1;Parent=MS.gene89327 |
| chr7.3 | exon | 42048984 | 42049191 | 42048984 | ID=MS.gene89327.t1.exon1;Parent=MS.gene89327.t1 |
| chr7.3 | CDS | 42048984 | 42049191 | 42048984 | ID=cds.MS.gene89327.t1;Parent=MS.gene89327.t1 |
| Gene Sequence |
| Protein sequence |
