AlfalfaGEDB | MS.gene90210

M. sativa cultivar XinJiangDaYe / MS.gene90210

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90210.t1	XP_003608424.1	97.1	35	1	0	1	35	1	35	3.00E-06	61.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90210.t1	G7JV73	97.1	35	1	0	1	35	1	35	2.2e-06	61.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04921	MS.gene90210	0.825934	3.58E-54	-1.69E-46
MS.gene050082	MS.gene90210	0.883433	4.51E-71	-1.69E-46
MS.gene051422	MS.gene90210	0.802158	6.41E-49	-1.69E-46
MS.gene053568	MS.gene90210	0.944796	1.01E-103	-1.69E-46
MS.gene053569	MS.gene90210	0.950354	1.96E-108	-1.69E-46
MS.gene057717	MS.gene90210	0.806951	6.41E-50	-1.69E-46
MS.gene058068	MS.gene90210	0.82517	5.43E-54	-1.69E-46
MS.gene061044	MS.gene90210	0.835665	1.47E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90210.t1	MTR_4g093940	92.969	128	4	1	1	128	1	123	2.55e-66	196
MS.gene90210.t1	MTR_4g093950	93.750	112	7	0	1	112	1	112	1.11e-49	155
MS.gene90210.t1	MTR_4g093910	92.857	112	8	0	1	112	1	112	8.66e-49	152
MS.gene90210.t1	MTR_4g093920	91.743	109	8	1	1	109	1	108	1.72e-47	149
MS.gene90210.t1	MTR_5g064530	64.474	152	28	4	1	128	1	150	1.06e-40	132
MS.gene90210.t1	MTR_5g064510	66.667	123	21	4	1	105	1	121	4.68e-36	120
MS.gene90210.t1	MTR_4g094678	69.620	79	6	3	17	92	1	64	2.17e-22	84.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 45 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTTCCTTGAGGCATATT+TGG	0.193132	4.2:-19778026	None:intergenic
GTGGGAATGGTAGGTAAATT+TGG	0.271860	4.2:-19777963	None:intergenic
GCAATAGATGGCGAGCTTCT+AGG	0.294792	4.2:-19777926	None:intergenic
GGTGGTAATGTAGTTGGTTT+TGG	0.298989	4.2:-19777990	None:intergenic
TAAAGCTGCAATGAAGGATT+TGG	0.355352	4.2:-19777874	None:intergenic
AGATGGAATTGTTGGAATAT+TGG	0.374933	4.2:-19778114	None:intergenic
GTGTTGTTGTTTGCAATAGA+TGG	0.376065	4.2:-19777938	None:intergenic
GGGAAAGTGGTTGGGATTGA+AGG	0.382492	4.2:-19778182	None:intergenic
GGCTAGGAGTTGAAGGTGGT+GGG	0.405223	4.2:-19778223	None:intergenic
TGAGGCATATTTGGAATTGA+AGG	0.405314	4.2:-19778017	None:intergenic
ATAGTGAGTTTAGGCATAGA+AGG	0.436994	4.2:-19778068	None:intergenic
GGGAAGGAGGGGAAAGTGGT+TGG	0.439412	4.2:-19778191	None:intergenic
GGAAGGAGGGGAAAGTGGTT+GGG	0.444157	4.2:-19778190	None:intergenic
GGCAAAGGTGGTAATGTAGT+TGG	0.444412	4.2:-19777996	None:intergenic
TCTAGAAGTGCTTAAGGGCT+AGG	0.453177	4.2:-19778239	None:intergenic
GGTTGGGATTGAAGGAAGGT+TGG	0.454159	4.2:-19778174	None:intergenic
AGTGGTGGCATAGTGAGTTT+AGG	0.457061	4.2:-19778077	None:intergenic
AAATGGGAAGGAGGGGAAAG+TGG	0.467197	4.2:-19778195	None:intergenic
GCTTAAGGGCTAGGAGTTGA+AGG	0.469112	4.2:-19778230	None:intergenic
GTTGGGATTGAAGGAAGGTT+GGG	0.469467	4.2:-19778173	None:intergenic
TTAGGCATAGAAGGGATAGT+AGG	0.471087	4.2:-19778059	None:intergenic
GGGCTAGGAGTTGAAGGTGG+TGG	0.483282	4.2:-19778224	None:intergenic
AAGAAGTAAAGCTGCAATGA+AGG	0.485067	4.2:-19777880	None:intergenic
GGAATATTGGTAGGAAGACT+TGG	0.503031	4.2:-19778101	None:intergenic
AAGTGGTTGGGATTGAAGGA+AGG	0.508086	4.2:-19778178	None:intergenic
TGCAATGAAGGATTTGGTTG+AGG	0.510590	4.2:-19777868	None:intergenic
TTTGGTAAAGTGGGAATGGT+AGG	0.519398	4.2:-19777972	None:intergenic
AGTGAAGTGGCTGCTGGCAA+TGG	0.520284	4.2:-19778149	None:intergenic
GGAATTGTTGGAATATTGGT+AGG	0.523994	4.2:-19778110	None:intergenic
ATGTTGAGAGATGGAATTGT+TGG	0.529497	4.2:-19778122	None:intergenic
TTGGTAGGAAGACTTGGAAG+TGG	0.530626	4.2:-19778095	None:intergenic
GAAGGGATAGTAGGCAATGG+AGG	0.560608	4.2:-19778050	None:intergenic
GAAGTGGCTGCTGGCAATGG+TGG	0.580562	4.2:-19778146	None:intergenic
TTTGGAATTGAAGGCAAAGG+TGG	0.580888	4.2:-19778008	None:intergenic
TTGGGAAGTGAAGTGGCTGC+TGG	0.584437	4.2:-19778155	None:intergenic
TCAATTCCAAATATGCCTCA+AGG	0.589431	4.2:+19778020	MS.gene90210:CDS
GCTAGGAGTTGAAGGTGGTG+GGG	0.597019	4.2:-19778222	None:intergenic
GTAGGAAGACTTGGAAGTGG+TGG	0.611131	4.2:-19778092	None:intergenic
ATATTTGGAATTGAAGGCAA+AGG	0.615183	4.2:-19778011	None:intergenic
TAGTGAGTTTAGGCATAGAA+GGG	0.616158	4.2:-19778067	None:intergenic
ATAGAAGGGATAGTAGGCAA+TGG	0.624968	4.2:-19778053	None:intergenic
AGGAAGGTTGGGAAGTGAAG+TGG	0.630070	4.2:-19778162	None:intergenic
AATGGTGGAATGTTGAGAGA+TGG	0.653205	4.2:-19778131	None:intergenic
AATGGAGGAAGATTTCCTTG+AGG	0.717776	4.2:-19778035	None:intergenic
TAAGGGCTAGGAGTTGAAGG+TGG	0.721449	4.2:-19778227	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATGGAATTGTTGGAATAT+TGG	-	chr4.2:19778117-19778136	None:intergenic	30.0%
!	ATATTTGGAATTGAAGGCAA+AGG	-	chr4.2:19778014-19778033	None:intergenic	30.0%
	AAGAAGTAAAGCTGCAATGA+AGG	-	chr4.2:19777883-19777902	None:intergenic	35.0%
	AGATTTCCTTGAGGCATATT+TGG	-	chr4.2:19778029-19778048	None:intergenic	35.0%
	ATAGTGAGTTTAGGCATAGA+AGG	-	chr4.2:19778071-19778090	None:intergenic	35.0%
	ATGTTGAGAGATGGAATTGT+TGG	-	chr4.2:19778125-19778144	None:intergenic	35.0%
	GGAATTGTTGGAATATTGGT+AGG	-	chr4.2:19778113-19778132	None:intergenic	35.0%
	TAAAGCTGCAATGAAGGATT+TGG	-	chr4.2:19777877-19777896	None:intergenic	35.0%
	TAGTGAGTTTAGGCATAGAA+GGG	-	chr4.2:19778070-19778089	None:intergenic	35.0%
	TCAATTCCAAATATGCCTCA+AGG	+	chr4.2:19778020-19778039	MS.gene90210:CDS	35.0%
!	TGAGGCATATTTGGAATTGA+AGG	-	chr4.2:19778020-19778039	None:intergenic	35.0%
!!	GTGTTGTTGTTTGCAATAGA+TGG	-	chr4.2:19777941-19777960	None:intergenic	35.0%
!!!	GTAGTTGGTTTTGGTAAAGT+GGG	-	chr4.2:19777984-19778003	None:intergenic	35.0%
!!!	TGTAGTTGGTTTTGGTAAAG+TGG	-	chr4.2:19777985-19778004	None:intergenic	35.0%
	AATGGAGGAAGATTTCCTTG+AGG	-	chr4.2:19778038-19778057	None:intergenic	40.0%
	ATAGAAGGGATAGTAGGCAA+TGG	-	chr4.2:19778056-19778075	None:intergenic	40.0%
	GGAATATTGGTAGGAAGACT+TGG	-	chr4.2:19778104-19778123	None:intergenic	40.0%
	GTGGGAATGGTAGGTAAATT+TGG	-	chr4.2:19777966-19777985	None:intergenic	40.0%
	TTAGGCATAGAAGGGATAGT+AGG	-	chr4.2:19778062-19778081	None:intergenic	40.0%
	TTTGGTAAAGTGGGAATGGT+AGG	-	chr4.2:19777975-19777994	None:intergenic	40.0%
!	AATGGTGGAATGTTGAGAGA+TGG	-	chr4.2:19778134-19778153	None:intergenic	40.0%
!	TGCAATGAAGGATTTGGTTG+AGG	-	chr4.2:19777871-19777890	None:intergenic	40.0%
!	TTTGGAATTGAAGGCAAAGG+TGG	-	chr4.2:19778011-19778030	None:intergenic	40.0%
!!	AAGGTGGTGGGGAAAAAAAT+GGG	-	chr4.2:19778214-19778233	None:intergenic	40.0%
!!	GGTGGTAATGTAGTTGGTTT+TGG	-	chr4.2:19777993-19778012	None:intergenic	40.0%
!!!	TGGTTTTGGTAAAGTGGGAA+TGG	-	chr4.2:19777979-19777998	None:intergenic	40.0%
	AAGTGGTTGGGATTGAAGGA+AGG	-	chr4.2:19778181-19778200	None:intergenic	45.0%
	AGTGGTGGCATAGTGAGTTT+AGG	-	chr4.2:19778080-19778099	None:intergenic	45.0%
	GGGAAAAAAATGGGAAGGAG+GGG	-	chr4.2:19778205-19778224	None:intergenic	45.0%
	GGGGAAAAAAATGGGAAGGA+GGG	-	chr4.2:19778206-19778225	None:intergenic	45.0%
	GTTGGGATTGAAGGAAGGTT+GGG	-	chr4.2:19778176-19778195	None:intergenic	45.0%
	TGGGGAAAAAAATGGGAAGG+AGG	-	chr4.2:19778207-19778226	None:intergenic	45.0%
	TGGTGGGGAAAAAAATGGGA+AGG	-	chr4.2:19778210-19778229	None:intergenic	45.0%
	TTGGTAGGAAGACTTGGAAG+TGG	-	chr4.2:19778098-19778117	None:intergenic	45.0%
!	GGCAAAGGTGGTAATGTAGT+TGG	-	chr4.2:19777999-19778018	None:intergenic	45.0%
!!	GAAGGTGGTGGGGAAAAAAA+TGG	-	chr4.2:19778215-19778234	None:intergenic	45.0%
	AAATGGGAAGGAGGGGAAAG+TGG	-	chr4.2:19778198-19778217	None:intergenic	50.0%
	AGGAAGGTTGGGAAGTGAAG+TGG	-	chr4.2:19778165-19778184	None:intergenic	50.0%
	GAAGGGATAGTAGGCAATGG+AGG	-	chr4.2:19778053-19778072	None:intergenic	50.0%
	GCAATAGATGGCGAGCTTCT+AGG	-	chr4.2:19777929-19777948	None:intergenic	50.0%
	GGGAAAGTGGTTGGGATTGA+AGG	-	chr4.2:19778185-19778204	None:intergenic	50.0%
	GGTTGGGATTGAAGGAAGGT+TGG	-	chr4.2:19778177-19778196	None:intergenic	50.0%
	GTAGGAAGACTTGGAAGTGG+TGG	-	chr4.2:19778095-19778114	None:intergenic	50.0%
	TAAGGGCTAGGAGTTGAAGG+TGG	-	chr4.2:19778230-19778249	None:intergenic	50.0%
	AGTGAAGTGGCTGCTGGCAA+TGG	-	chr4.2:19778152-19778171	None:intergenic	55.0%
	GCTAGGAGTTGAAGGTGGTG+GGG	-	chr4.2:19778225-19778244	None:intergenic	55.0%
	GGAAGGAGGGGAAAGTGGTT+GGG	-	chr4.2:19778193-19778212	None:intergenic	55.0%
	GGCTAGGAGTTGAAGGTGGT+GGG	-	chr4.2:19778226-19778245	None:intergenic	55.0%
	TTGGGAAGTGAAGTGGCTGC+TGG	-	chr4.2:19778158-19778177	None:intergenic	55.0%
	GAAGTGGCTGCTGGCAATGG+TGG	-	chr4.2:19778149-19778168	None:intergenic	60.0%
	GGGAAGGAGGGGAAAGTGGT+TGG	-	chr4.2:19778194-19778213	None:intergenic	60.0%
	GGGCTAGGAGTTGAAGGTGG+TGG	-	chr4.2:19778227-19778246	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	19777864	19778250	19777864	ID=MS.gene90210
chr4.2	mRNA	19777864	19778250	19777864	ID=MS.gene90210.t1;Parent=MS.gene90210
chr4.2	exon	19777864	19778250	19777864	ID=MS.gene90210.t1.exon1;Parent=MS.gene90210.t1
chr4.2	CDS	19777864	19778250	19777864	ID=cds.MS.gene90210.t1;Parent=MS.gene90210.t1

Gene Sequence

>MS.gene90210

ATGGCCTCAACCAAATCCTTCATTGCAGCTTTACTTCTTGTTGTCACAATGTCAAGCATGAGCCTAGAAGCTCGCCATCTATTGCAAACAACAACACAACCAAATTTACCTACCATTCCCACTTTACCAAAACCAACTACATTACCACCTTTGCCTTCAATTCCAAATATGCCTCAAGGAAATCTTCCTCCATTGCCTACTATCCCTTCTATGCCTAAACTCACTATGCCACCACTTCCAAGTCTTCCTACCAATATTCCAACAATTCCATCTCTCAACATTCCACCATTGCCAGCAGCCACTTCACTTCCCAACCTTCCTTCAATCCCAACCACTTTCCCCTCCTTCCCATTTTTTTCCCCACCACCTTCAACTCCTAGCCCTTAA

Protein sequence

>MS.gene90210.t1

MASTKSFIAALLLVVTMSSMSLEARHLLQTTTQPNLPTIPTLPKPTTLPPLPSIPNMPQGNLPPLPTIPSMPKLTMPPLPSLPTNIPTIPSLNIPPLPAATSLPNLPSIPTTFPSFPFFSPPPSTPSP