Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90346.t1 | XP_013448823.1 | 95.2 | 83 | 4 | 0 | 1 | 83 | 38 | 120 | 1.30E-37 | 165.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90346.t1 | P81748 | 34.7 | 75 | 49 | 0 | 1 | 75 | 37 | 111 | 2.0e-07 | 56.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90346.t1 | A0A072UAN8 | 95.2 | 83 | 4 | 0 | 1 | 83 | 38 | 120 | 9.1e-38 | 165.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050301 | MS.gene90346 | 0.803545 | 3.31E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene90346 | 0.804125 | 2.51E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene90346 | -0.815659 | 8.25E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene90346 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene90346 | 0.843516 | 1.34E-58 | -1.69E-46 |
| MS.gene054183 | MS.gene90346 | 0.824981 | 6.01E-54 | -1.69E-46 |
| MS.gene055116 | MS.gene90346 | 0.834643 | 2.66E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene90346 | 0.812305 | 4.53E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene90346 | 0.805432 | 1.34E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene90346 | 0.8251 | 5.63E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene90346 | 0.847005 | 1.52E-59 | -1.69E-46 |
| MS.gene059462 | MS.gene90346 | 0.80662 | 7.53E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene90346 | 0.81535 | 9.67E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene90346 | 0.809612 | 1.74E-50 | -1.69E-46 |
| MS.gene059695 | MS.gene90346 | 0.818341 | 2.06E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene90346 | 0.808437 | 3.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90346.t1 | MTR_7g059460 | 95.181 | 83 | 4 | 0 | 1 | 83 | 38 | 120 | 1.51e-53 | 162 |
| MS.gene90346.t1 | MTR_7g059460 | 97.015 | 67 | 2 | 0 | 1 | 67 | 38 | 104 | 5.86e-42 | 132 |
| MS.gene90346.t1 | MTR_4g014040 | 65.333 | 75 | 26 | 0 | 1 | 75 | 38 | 112 | 1.06e-30 | 104 |
| MS.gene90346.t1 | MTR_4g014040 | 62.857 | 70 | 26 | 0 | 1 | 70 | 38 | 107 | 1.94e-27 | 96.7 |
| MS.gene90346.t1 | MTR_4g014040 | 64.706 | 68 | 24 | 0 | 1 | 68 | 38 | 105 | 3.53e-26 | 94.4 |
| MS.gene90346.t1 | MTR_4g127370 | 56.000 | 75 | 33 | 0 | 1 | 75 | 776 | 850 | 2.27e-22 | 89.4 |
| MS.gene90346.t1 | MTR_4g127360 | 48.000 | 75 | 39 | 0 | 1 | 75 | 38 | 112 | 3.36e-20 | 77.8 |
| MS.gene90346.t1 | MTR_4g013730 | 44.615 | 65 | 36 | 0 | 1 | 65 | 38 | 102 | 4.19e-16 | 67.4 |
| MS.gene90346.t1 | MTR_0562s0020 | 44.615 | 65 | 36 | 0 | 1 | 65 | 38 | 102 | 4.19e-16 | 67.4 |
| MS.gene90346.t1 | MTR_7g070250 | 39.130 | 69 | 42 | 0 | 1 | 69 | 38 | 106 | 5.91e-15 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90346.t1 | AT5G01650 | 61.333 | 75 | 29 | 0 | 1 | 75 | 38 | 112 | 1.50e-28 | 99.4 |
| MS.gene90346.t1 | AT5G01650 | 60.563 | 71 | 28 | 0 | 1 | 71 | 38 | 108 | 4.72e-27 | 95.5 |
| MS.gene90346.t1 | AT5G01650 | 60.000 | 70 | 28 | 0 | 1 | 70 | 38 | 107 | 2.87e-26 | 93.6 |
| MS.gene90346.t1 | AT5G01650 | 60.000 | 70 | 28 | 0 | 1 | 70 | 38 | 107 | 2.87e-26 | 93.6 |
| MS.gene90346.t1 | AT5G57170 | 50.667 | 75 | 37 | 0 | 1 | 75 | 38 | 112 | 4.45e-19 | 75.1 |
| MS.gene90346.t1 | AT5G57170 | 53.846 | 65 | 30 | 0 | 1 | 65 | 38 | 102 | 1.00e-18 | 74.3 |
Find 20 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAGTTTGTTGTTCAAATC+TGG | 0.254690 | 7.4:+43622366 | None:intergenic |
| ACAAGTTTCTCGGAATCATT+TGG | 0.359454 | 7.4:+43622109 | None:intergenic |
| GTTATAATTGTGGACTGAAT+GGG | 0.367727 | 7.4:-43622151 | MS.gene90346:CDS |
| GGATCAATACCAATATGTTT+TGG | 0.376407 | 7.4:-43622444 | MS.gene90346:CDS |
| TCAGTTTCACCAAAACATAT+TGG | 0.384156 | 7.4:+43622435 | None:intergenic |
| GGTTATAATTGTGGACTGAA+TGG | 0.389437 | 7.4:-43622152 | MS.gene90346:CDS |
| TATGGTGAATTTGTGGCTAT+TGG | 0.409602 | 7.4:-43622399 | MS.gene90346:CDS |
| TATGTGACGGTTTCATTAGA+AGG | 0.443254 | 7.4:-43622465 | MS.gene90346:CDS |
| AGTTCTTCACACCATGTTGT+CGG | 0.443341 | 7.4:-43622328 | MS.gene90346:CDS |
| ACTGAGGAGCCAGCAGCATA+TGG | 0.461454 | 7.4:-43622417 | MS.gene90346:CDS |
| AATTTGTAGGGTTATAATTG+TGG | 0.473862 | 7.4:-43622161 | MS.gene90346:intron |
| CTTAAAATTCTATGACATTG+AGG | 0.475447 | 7.4:-43622286 | MS.gene90346:intron |
| AGCAGCATATGGTGAATTTG+TGG | 0.510208 | 7.4:-43622406 | MS.gene90346:CDS |
| ACAAATTCACCATATGCTGC+TGG | 0.542569 | 7.4:+43622408 | None:intergenic |
| TTATAATTGTGGACTGAATG+GGG | 0.546367 | 7.4:-43622150 | MS.gene90346:CDS |
| CAACATGGTGTGAAGAACTA+AGG | 0.556695 | 7.4:+43622332 | None:intergenic |
| TTTAAGTAGAAACGTGACTT+GGG | 0.574926 | 7.4:+43622303 | None:intergenic |
| TGACTTGGGCACCGACAACA+TGG | 0.611246 | 7.4:+43622317 | None:intergenic |
| TATAATTGTGGACTGAATGG+GGG | 0.631220 | 7.4:-43622149 | MS.gene90346:CDS |
| TATTGATTTGCAGTATGTGA+CGG | 0.638900 | 7.4:-43622478 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCAAAATAGTAAATGT+TGG | + | chr7.4:43622331-43622350 | None:intergenic | 20.0% |
| !!! | CATATGTTTGTTAATTTGTA+GGG | - | chr7.4:43622416-43622435 | MS.gene90346:CDS | 20.0% |
| !!! | TCATATGTTTGTTAATTTGT+AGG | - | chr7.4:43622415-43622434 | MS.gene90346:CDS | 20.0% |
| ! | AATTTGTAGGGTTATAATTG+TGG | - | chr7.4:43622428-43622447 | MS.gene90346:CDS | 25.0% |
| ! | CTTAAAATTCTATGACATTG+AGG | - | chr7.4:43622303-43622322 | MS.gene90346:CDS | 25.0% |
| TCAAGAATAGTAAATGAGAG+TGG | + | chr7.4:43622376-43622395 | None:intergenic | 30.0% | |
| TCAGTTTCACCAAAACATAT+TGG | + | chr7.4:43622157-43622176 | None:intergenic | 30.0% | |
| TTTAAGTAGAAACGTGACTT+GGG | + | chr7.4:43622289-43622308 | None:intergenic | 30.0% | |
| ! | GGATCAATACCAATATGTTT+TGG | - | chr7.4:43622145-43622164 | MS.gene90346:CDS | 30.0% |
| ! | GTTATAATTGTGGACTGAAT+GGG | - | chr7.4:43622438-43622457 | MS.gene90346:CDS | 30.0% |
| ! | TTATAATTGTGGACTGAATG+GGG | - | chr7.4:43622439-43622458 | MS.gene90346:CDS | 30.0% |
| !! | CTTAGTTTGTTGTTCAAATC+TGG | + | chr7.4:43622226-43622245 | None:intergenic | 30.0% |
| !! | TTTTAAGTAGAAACGTGACT+TGG | + | chr7.4:43622290-43622309 | None:intergenic | 30.0% |
| !!! | AATATGTTTTGGTGAAACTG+AGG | - | chr7.4:43622156-43622175 | MS.gene90346:intron | 30.0% |
| TATGGTGAATTTGTGGCTAT+TGG | - | chr7.4:43622190-43622209 | MS.gene90346:intron | 35.0% | |
| TATGTGACGGTTTCATTAGA+AGG | - | chr7.4:43622124-43622143 | MS.gene90346:CDS | 35.0% | |
| ! | GGTTATAATTGTGGACTGAA+TGG | - | chr7.4:43622437-43622456 | MS.gene90346:CDS | 35.0% |
| ! | TATAATTGTGGACTGAATGG+GGG | - | chr7.4:43622440-43622459 | MS.gene90346:CDS | 35.0% |
| ACAAATTCACCATATGCTGC+TGG | + | chr7.4:43622184-43622203 | None:intergenic | 40.0% | |
| AGCAGCATATGGTGAATTTG+TGG | - | chr7.4:43622183-43622202 | MS.gene90346:intron | 40.0% | |
| AGTTCTTCACACCATGTTGT+CGG | - | chr7.4:43622261-43622280 | MS.gene90346:intron | 40.0% | |
| CAACATGGTGTGAAGAACTA+AGG | + | chr7.4:43622260-43622279 | None:intergenic | 40.0% | |
| ACTGAGGAGCCAGCAGCATA+TGG | - | chr7.4:43622172-43622191 | MS.gene90346:intron | 55.0% | |
| !! | TGACTTGGGCACCGACAACA+TGG | + | chr7.4:43622275-43622294 | None:intergenic | 55.0% |
| ACTGAATGGGGGTCTCGTGG+TGG | - | chr7.4:43622451-43622470 | MS.gene90346:CDS | 60.0% | |
| TGGACTGAATGGGGGTCTCG+TGG | - | chr7.4:43622448-43622467 | MS.gene90346:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 43622124 | 43622487 | 43622124 | ID=MS.gene90346 |
| chr7.4 | mRNA | 43622124 | 43622487 | 43622124 | ID=MS.gene90346.t1;Parent=MS.gene90346 |
| chr7.4 | exon | 43622287 | 43622487 | 43622287 | ID=MS.gene90346.t1.exon1;Parent=MS.gene90346.t1 |
| chr7.4 | CDS | 43622287 | 43622487 | 43622287 | ID=cds.MS.gene90346.t1;Parent=MS.gene90346.t1 |
| chr7.4 | exon | 43622124 | 43622174 | 43622124 | ID=MS.gene90346.t1.exon2;Parent=MS.gene90346.t1 |
| chr7.4 | CDS | 43622124 | 43622174 | 43622124 | ID=cds.MS.gene90346.t1;Parent=MS.gene90346.t1 |
| Gene Sequence |
| Protein sequence |
