AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90909

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90909.t1 MTR_1g117010 90.574 244 11 2 2 235 3 244 2.61e-155 431
MS.gene90909.t1 MTR_1g117010 90.574 244 11 2 2 235 3 244 2.61e-155 431
MS.gene90909.t1 MTR_7g110980 61.026 195 73 1 40 234 68 259 1.64e-83 249
MS.gene90909.t1 MTR_1g085560 60.000 210 76 3 30 234 43 249 4.19e-83 248
MS.gene90909.t1 MTR_3g463900 62.147 177 56 3 65 234 31 203 4.95e-70 213
MS.gene90909.t1 MTR_3g117750 59.649 171 58 3 69 232 13 179 7.97e-66 202
MS.gene90909.t1 MTR_3g070500 53.846 208 71 5 46 232 28 231 5.87e-65 201
MS.gene90909.t1 MTR_7g116340 58.480 171 60 3 69 232 5 171 6.66e-65 199
MS.gene90909.t1 MTR_4g075290 59.195 174 60 3 66 232 2 171 3.07e-64 197
MS.gene90909.t1 MTR_8g079690 55.814 172 65 2 69 232 5 173 5.55e-58 187
MS.gene90909.t1 MTR_4g073650 47.938 194 91 2 47 233 25 215 2.16e-56 179
MS.gene90909.t1 MTR_4g086760 53.757 173 69 2 69 233 5 174 2.65e-56 182
MS.gene90909.t1 MTR_8g040180 51.613 186 81 3 53 232 3 185 2.86e-56 177
MS.gene90909.t1 MTR_4g086760 53.757 173 69 2 69 233 5 174 4.02e-56 182
MS.gene90909.t1 MTR_4g086760 53.757 173 69 2 69 233 5 174 4.14e-56 183
MS.gene90909.t1 MTR_7g095750 55.556 171 64 3 70 232 9 175 2.26e-53 182
MS.gene90909.t1 MTR_3g070500 54.268 164 51 4 46 188 28 188 5.72e-49 159
MS.gene90909.t1 MTR_3g085705 72.642 106 25 2 115 220 24 125 8.85e-46 149
MS.gene90909.t1 MTR_2g085075 52.778 144 63 3 84 226 470 609 1.74e-40 146
MS.gene90909.t1 MTR_7g105220 47.917 144 70 4 84 226 18 157 1.12e-36 127
MS.gene90909.t1 MTR_3g070500 51.064 141 45 4 46 165 28 165 1.13e-35 124
MS.gene90909.t1 MTR_3g107660 44.444 171 83 3 70 232 12 178 7.30e-35 131
MS.gene90909.t1 MTR_3g070500 51.449 138 43 4 46 162 28 162 1.53e-34 121
MS.gene90909.t1 MTR_8g036015 47.945 146 69 4 84 227 9 149 7.27e-34 119
MS.gene90909.t1 MTR_1g011980 46.000 150 67 4 70 210 9 153 3.92e-31 120
MS.gene90909.t1 MTR_5g015500 46.528 144 72 4 85 227 314 453 5.93e-31 119
MS.gene90909.t1 MTR_5g015500 46.528 144 72 4 85 227 354 493 1.05e-30 119
MS.gene90909.t1 MTR_2g023120 42.143 140 74 6 85 221 22 157 1.02e-19 87.8
MS.gene90909.t1 MTR_6g084140 39.474 152 79 5 84 226 26 173 1.55e-19 87.4
MS.gene90909.t1 MTR_6g084140 39.474 152 79 5 84 226 9 156 1.64e-19 87.4
MS.gene90909.t1 MTR_8g442420 38.816 152 80 5 84 226 9 156 3.46e-18 83.6
MS.gene90909.t1 MTR_2g461260 27.049 244 116 10 54 235 44 287 1.71e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90909.t1 AT5G13120 80.347 173 34 0 63 235 81 253 4.05e-107 309
MS.gene90909.t1 AT5G13120 80.347 173 34 0 63 235 85 257 4.39e-107 309
MS.gene90909.t1 AT3G62030 64.249 193 65 2 43 234 69 258 1.35e-86 257
MS.gene90909.t1 AT3G62030 63.542 192 67 1 43 234 69 257 1.56e-86 257
MS.gene90909.t1 AT3G62030 64.249 193 65 2 43 234 122 311 1.21e-85 257
MS.gene90909.t1 AT2G29960 61.017 177 58 3 65 234 29 201 3.53e-69 211
MS.gene90909.t1 AT2G29960 61.017 177 58 3 65 234 29 201 3.53e-69 211
MS.gene90909.t1 AT5G58710 60.452 177 59 3 65 234 32 204 4.95e-69 211
MS.gene90909.t1 AT2G21130 60.819 171 56 3 69 232 6 172 5.26e-68 207
MS.gene90909.t1 AT2G16600 60.234 171 57 3 69 232 6 172 7.63e-67 204
MS.gene90909.t1 AT4G38740 59.064 171 59 3 69 232 5 171 1.26e-65 201
MS.gene90909.t1 AT3G56070 57.310 171 62 3 69 232 5 171 5.65e-64 197
MS.gene90909.t1 AT3G56070 57.310 171 62 3 69 232 5 171 5.65e-64 197
MS.gene90909.t1 AT4G34870 58.480 171 60 3 69 232 5 171 3.30e-62 192
MS.gene90909.t1 AT3G55920 57.062 177 63 4 66 234 57 228 4.51e-62 194
MS.gene90909.t1 AT2G29960 57.627 177 54 4 65 234 29 191 3.43e-61 190
MS.gene90909.t1 AT4G34960 50.265 189 84 2 52 233 31 216 7.95e-57 181
MS.gene90909.t1 AT2G15790 56.069 173 65 2 69 233 5 174 1.29e-56 184
MS.gene90909.t1 AT2G38730 54.438 169 68 3 70 232 34 199 3.82e-56 178
MS.gene90909.t1 AT2G16600 55.488 164 54 3 69 232 6 150 8.11e-56 175
MS.gene90909.t1 AT3G63400 57.647 170 60 3 70 231 9 174 9.76e-54 177
MS.gene90909.t1 AT3G63400 57.647 170 60 3 70 231 9 174 3.80e-52 177
MS.gene90909.t1 AT3G63400 57.647 170 60 3 70 231 9 174 3.80e-52 177
MS.gene90909.t1 AT3G63400 57.647 170 60 3 70 231 9 174 3.80e-52 177
MS.gene90909.t1 AT4G34960 51.389 144 60 2 52 188 31 171 5.14e-42 141
MS.gene90909.t1 AT3G44600 52.778 144 63 3 84 226 484 623 9.30e-41 147
MS.gene90909.t1 AT2G36130 46.528 144 72 4 84 226 18 157 1.24e-37 129
MS.gene90909.t1 AT1G01940 46.897 145 72 4 84 227 9 149 2.07e-35 124
MS.gene90909.t1 AT3G22920 44.828 174 75 7 69 232 5 167 4.63e-32 117
MS.gene90909.t1 AT5G67530 46.154 143 72 4 85 226 353 491 1.69e-31 121
MS.gene90909.t1 AT4G32420 41.243 177 90 4 65 232 4 175 7.88e-28 111
MS.gene90909.t1 AT4G32420 41.243 177 90 4 65 232 4 175 7.88e-28 111
MS.gene90909.t1 AT4G32420 41.243 177 90 4 65 232 4 175 7.88e-28 111
MS.gene90909.t1 AT4G32420 41.243 177 90 4 65 232 4 175 7.88e-28 111
MS.gene90909.t1 AT4G32420 41.935 155 77 3 65 210 4 154 6.79e-26 105
MS.gene90909.t1 AT1G53720 40.132 152 78 5 84 226 9 156 1.18e-22 94.7
MS.gene90909.t1 AT4G33060 43.262 141 71 7 85 221 22 157 3.94e-22 94.7
MS.gene90909.t1 AT1G53720 40.132 152 78 5 84 226 9 156 9.95e-22 93.6

Find 74 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGATTGTCACCTTCTTT+AGG 0.241303 1.2:+86824585 None:intergenic
GATCAGAGGCATGTTGTATT+TGG 0.249985 1.2:-86824677 MS.gene90909:CDS
ACTGATTGTCACCTTCTTTA+GGG 0.273487 1.2:+86824586 None:intergenic
TGGAAGGCATGGACATTGTT+AGG 0.314434 1.2:-86824646 MS.gene90909:CDS
CAAGGAGGAGACTTTGATAA+AGG 0.345816 1.2:-86825200 MS.gene90909:CDS
TGTTGTATTTGGCCAAGTTT+TGG 0.352436 1.2:-86824666 MS.gene90909:CDS
TCCACCAGTGAGAAGGGCTT+TGG 0.372862 1.2:-86825269 MS.gene90909:CDS
ATCTATCTATCGCAGGGAAC+TGG 0.385423 1.2:-86825108 MS.gene90909:intron
AACAAAACATGTCAAGCAAT+TGG 0.393584 1.2:+86824548 None:intergenic
CTTCCAACCAGCTTCCCAAC+TGG 0.403156 1.2:+86825353 None:intergenic
GCATCTCCGTCGGGGTGGTT+AGG 0.409454 1.2:-86825523 MS.gene90909:CDS
GGAAGTTCTCAACAGTTTGC+GGG 0.417261 1.2:+86825300 None:intergenic
AAAGTGAGGGCTGTGGAAAA+TGG 0.427415 1.2:-86825452 MS.gene90909:CDS
GTATTCTTTGATGTTAGTAT+TGG 0.430298 1.2:-86825380 MS.gene90909:CDS
AACTGACTCCCATTGGTGTT+GGG 0.430322 1.2:+86824815 None:intergenic
GGTGGTTAGGTCCCGAGGTG+TGG 0.434983 1.2:-86825510 MS.gene90909:CDS
AGGATTGTGATAGGACTCTT+TGG 0.436041 1.2:-86825332 MS.gene90909:CDS
GCCCTTCTCACTGGTGGAAA+GGG 0.436297 1.2:+86825274 None:intergenic
GTGGTTAGGTCCCGAGGTGT+GGG 0.453738 1.2:-86825509 MS.gene90909:CDS
TGGAAATCCAGTTGGGAAGC+TGG 0.456366 1.2:-86825360 MS.gene90909:CDS
TGCCCTTTCCACCAGTGAGA+AGG 0.468665 1.2:-86825276 MS.gene90909:CDS
CTAAAAGAAGCATCTCCGTC+GGG 0.471283 1.2:-86825532 MS.gene90909:CDS
TGTCCATGCCTTCCAAAACT+TGG 0.486542 1.2:+86824654 None:intergenic
ACTGGAGGCAAAAGTATCTC+TGG 0.489609 1.2:-86825090 MS.gene90909:CDS
AGCCCTTCTCACTGGTGGAA+AGG 0.493291 1.2:+86825273 None:intergenic
ATCAAGGACTTCATGATTCA+AGG 0.494593 1.2:-86825218 MS.gene90909:CDS
ACATTGGCAGACGCTGTGGC+TGG 0.505086 1.2:-86824699 MS.gene90909:intron
GCCCTTTCCACCAGTGAGAA+GGG 0.506529 1.2:-86825275 MS.gene90909:CDS
CATGCTAACAGCTCCTGGTC+CGG 0.506814 1.2:+86824850 None:intergenic
ATGCTGGTCCCAACACCAAT+GGG 0.515041 1.2:-86824823 MS.gene90909:CDS
CACCTTCCATCGCGTCATCA+AGG 0.515078 1.2:-86825234 MS.gene90909:CDS
GTTAGTATTGGAAATCCAGT+TGG 0.515418 1.2:-86825368 MS.gene90909:CDS
CCAGTGTCTCATACCGGACC+AGG 0.515423 1.2:-86824863 MS.gene90909:intron
TGTTAGGTCGATTGAATCAC+GGG 0.517014 1.2:-86824630 MS.gene90909:CDS
CTGATTGTCACCTTCTTTAG+GGG 0.526723 1.2:+86824587 None:intergenic
CGGAAGTTCTCAACAGTTTG+CGG 0.530427 1.2:+86825299 None:intergenic
TTTGCCATGCTAACAGCTCC+TGG 0.534982 1.2:+86824845 None:intergenic
GCTGTTAGCATGGCAAATGC+TGG 0.538859 1.2:-86824839 MS.gene90909:CDS
TAGTTTCACACCCACACCTC+GGG 0.541866 1.2:+86825499 None:intergenic
AATGCTGGTCCCAACACCAA+TGG 0.549301 1.2:-86824824 MS.gene90909:CDS
GCCAAAGCCCTTCTCACTGG+TGG 0.549951 1.2:+86825268 None:intergenic
GTAGCCAAAGCCCTTCTCAC+TGG 0.550204 1.2:+86825265 None:intergenic
CAGTTGGGAAGCTGGTTGGA+AGG 0.554098 1.2:-86825352 MS.gene90909:CDS
TTAGTATTGGAAATCCAGTT+GGG 0.555401 1.2:-86825367 MS.gene90909:CDS
CTTGATGACGCGATGGAAGG+TGG 0.555450 1.2:+86825235 None:intergenic
CTGGTTGGAAGGATTGTGAT+AGG 0.560590 1.2:-86825341 MS.gene90909:CDS
AAACTGACTCCCATTGGTGT+TGG 0.561474 1.2:+86824814 None:intergenic
TGGTGATCGTCCCCTAAAGA+AGG 0.561937 1.2:-86824597 MS.gene90909:CDS
AATCCAGTTGGGAAGCTGGT+TGG 0.562899 1.2:-86825356 MS.gene90909:CDS
AGACGCTGTGGCTGGATCAG+AGG 0.565181 1.2:-86824691 MS.gene90909:CDS
GTCGGGGTGGTTAGGTCCCG+AGG 0.565995 1.2:-86825515 MS.gene90909:CDS
TTGAATGCAGCAACAGCCAT+CGG 0.590024 1.2:+86825756 None:intergenic
TTGTTAGGTCGATTGAATCA+CGG 0.592054 1.2:-86824631 MS.gene90909:CDS
TCTCACTGGTGGAAAGGGCA+CGG 0.601235 1.2:+86825279 None:intergenic
CGGACCAGGAGCTGTTAGCA+TGG 0.603520 1.2:-86824849 MS.gene90909:CDS
CCTAAAAGAAGCATCTCCGT+CGG 0.606730 1.2:-86825533 MS.gene90909:CDS
ACAAGTGAAAGTGAGGGCTG+TGG 0.614839 1.2:-86825459 MS.gene90909:CDS
CATGACATTGGCAGACGCTG+TGG 0.616067 1.2:-86824703 MS.gene90909:intron
CTAGTTTCACACCCACACCT+CGG 0.617486 1.2:+86825498 None:intergenic
TACGACGACAAGTGAAAGTG+AGG 0.618754 1.2:-86825466 MS.gene90909:CDS
GTCCTTGATGACGCGATGGA+AGG 0.624852 1.2:+86825232 None:intergenic
TGAAGTCCTTGATGACGCGA+TGG 0.626809 1.2:+86825228 None:intergenic
ATATCTCCAGTGTCTCATAC+CGG 0.627003 1.2:-86824869 MS.gene90909:intron
GATGACGCGATGGAAGGTGG+AGG 0.631465 1.2:+86825238 None:intergenic
GTGGGTGTGAAACTAGCAGT+AGG 0.631670 1.2:-86825491 MS.gene90909:CDS
TATCTATCGCAGGGAACTGG+AGG 0.635120 1.2:-86825105 MS.gene90909:intron
GGAAAATGGTAGTAGTTCAG+AGG 0.650163 1.2:-86825438 MS.gene90909:CDS
GAATCACGGGAGACTGATCG+TGG 0.651487 1.2:-86824617 MS.gene90909:CDS
TCGGGACCTAACCACCCCGA+CGG 0.658560 1.2:+86825517 None:intergenic
AAGAAGCATCTCCGTCGGGG+TGG 0.659605 1.2:-86825528 MS.gene90909:CDS
AAGGTGACAATCAGTGACTG+TGG 0.689468 1.2:-86824578 MS.gene90909:CDS
TAAAAGAAGCATCTCCGTCG+GGG 0.693386 1.2:-86825531 MS.gene90909:CDS
ACGACGACAAGTGAAAGTGA+GGG 0.705755 1.2:-86825465 MS.gene90909:CDS
AAGGACTTCATGATTCAAGG+AGG 0.745105 1.2:-86825215 MS.gene90909:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATCAATTAAATTTAAGTAA+TGG + chr1.2:86824678-86824697 None:intergenic 15.0%
!!! ATAAGTTGATGAATAAAAAA+AGG + chr1.2:86825174-86825193 None:intergenic 15.0%
!! AAATAAAATAAACACAGTGA+AGG + chr1.2:86825346-86825365 None:intergenic 20.0%
!! AATAAAATAAACACAGTGAA+GGG + chr1.2:86825345-86825364 None:intergenic 20.0%
!! TATTAATTGATTGATTGTCT+TGG - chr1.2:86824711-86824730 MS.gene90909:intron 20.0%
!!! AGTTTAGTTTAGTTTAGTTT+AGG - chr1.2:86825367-86825386 MS.gene90909:CDS 20.0%
! ATAAAATAAACACAGTGAAG+GGG + chr1.2:86825344-86825363 None:intergenic 25.0%
! ATATATATCAGTGAGAAAAG+TGG + chr1.2:86824598-86824617 None:intergenic 25.0%
! ATTCTTCTATTCTCTCTTAT+CGG - chr1.2:86825545-86825564 MS.gene90909:CDS 25.0%
! GTATTCTTTGATGTTAGTAT+TGG - chr1.2:86824932-86824951 MS.gene90909:intron 25.0%
!! GTTGATGAATAAAAAAAGGA+AGG + chr1.2:86825170-86825189 None:intergenic 25.0%
AATTGATTGATTGTCTTGGT+TGG - chr1.2:86824715-86824734 MS.gene90909:intron 30.0%
ACTTAATTGTCTCATGACAT+TGG - chr1.2:86825597-86825616 MS.gene90909:intron 30.0%
ATTGATTGATTGTCTTGGTT+GGG - chr1.2:86824716-86824735 MS.gene90909:intron 30.0%
TAAAATAAACACAGTGAAGG+GGG + chr1.2:86825343-86825362 None:intergenic 30.0%
! TTAGTATTGGAAATCCAGTT+GGG - chr1.2:86824945-86824964 MS.gene90909:intron 30.0%
ACTGATTGTCACCTTCTTTA+GGG + chr1.2:86825729-86825748 None:intergenic 35.0%
ATCAAGGACTTCATGATTCA+AGG - chr1.2:86825094-86825113 MS.gene90909:CDS 35.0%
CTATCTATCTATCTATCGCA+GGG - chr1.2:86825198-86825217 MS.gene90909:CDS 35.0%
TATATCAGTGAGAAAAGTGG+CGG + chr1.2:86824595-86824614 None:intergenic 35.0%
TCTATCTATCTATCTATCGC+AGG - chr1.2:86825197-86825216 MS.gene90909:CDS 35.0%
TTGTTAGGTCGATTGAATCA+CGG - chr1.2:86825681-86825700 MS.gene90909:intron 35.0%
! GTTAGTATTGGAAATCCAGT+TGG - chr1.2:86824944-86824963 MS.gene90909:intron 35.0%
! TATGAAAAACTGACTCCCAT+TGG + chr1.2:86825507-86825526 None:intergenic 35.0%
! TGTTGTATTTGGCCAAGTTT+TGG - chr1.2:86825646-86825665 MS.gene90909:intron 35.0%
!!! CTTCTTTTAGGGTTAGGATT+AGG + chr1.2:86824770-86824789 None:intergenic 35.0%
AAGGACTTCATGATTCAAGG+AGG - chr1.2:86825097-86825116 MS.gene90909:intron 40.0%
AGGATTGTGATAGGACTCTT+TGG - chr1.2:86824980-86824999 MS.gene90909:intron 40.0%
ATATCTCCAGTGTCTCATAC+CGG - chr1.2:86825443-86825462 MS.gene90909:CDS 40.0%
CAAGGAGGAGACTTTGATAA+AGG - chr1.2:86825112-86825131 MS.gene90909:intron 40.0%
CTGATTGTCACCTTCTTTAG+GGG + chr1.2:86825728-86825747 None:intergenic 40.0%
GGAAAATGGTAGTAGTTCAG+AGG - chr1.2:86824874-86824893 MS.gene90909:intron 40.0%
TGTTAGGTCGATTGAATCAC+GGG - chr1.2:86825682-86825701 MS.gene90909:intron 40.0%
! CACTGATTGTCACCTTCTTT+AGG + chr1.2:86825730-86825749 None:intergenic 40.0%
! GATCAGAGGCATGTTGTATT+TGG - chr1.2:86825635-86825654 MS.gene90909:intron 40.0%
! GTTTTTCATATGCGCTGTCA+AGG - chr1.2:86825517-86825536 MS.gene90909:CDS 40.0%
!!! GAGATGCTTCTTTTAGGGTT+AGG + chr1.2:86824776-86824795 None:intergenic 40.0%
!!! GTATTTGGCCAAGTTTTGGA+AGG - chr1.2:86825650-86825669 MS.gene90909:intron 40.0%
AAAGTGAGGGCTGTGGAAAA+TGG - chr1.2:86824860-86824879 MS.gene90909:CDS 45.0%
AAGGTGACAATCAGTGACTG+TGG - chr1.2:86825734-86825753 MS.gene90909:intron 45.0%
ACGACGACAAGTGAAAGTGA+GGG - chr1.2:86824847-86824866 MS.gene90909:CDS 45.0%
ATCTATCTATCGCAGGGAAC+TGG - chr1.2:86825204-86825223 MS.gene90909:CDS 45.0%
CGGAAGTTCTCAACAGTTTG+CGG + chr1.2:86825016-86825035 None:intergenic 45.0%
CTGGTTGGAAGGATTGTGAT+AGG - chr1.2:86824971-86824990 MS.gene90909:intron 45.0%
GATTGATTGTCTTGGTTGGG+TGG - chr1.2:86824719-86824738 MS.gene90909:intron 45.0%
GGAAGTTCTCAACAGTTTGC+GGG + chr1.2:86825015-86825034 None:intergenic 45.0%
TACGACGACAAGTGAAAGTG+AGG - chr1.2:86824846-86824865 MS.gene90909:CDS 45.0%
TGTCCATGCCTTCCAAAACT+TGG + chr1.2:86825661-86825680 None:intergenic 45.0%
! AAACTGACTCCCATTGGTGT+TGG + chr1.2:86825501-86825520 None:intergenic 45.0%
! AACTGACTCCCATTGGTGTT+GGG + chr1.2:86825500-86825519 None:intergenic 45.0%
! CGACGGAGATGCTTCTTTTA+GGG + chr1.2:86824781-86824800 None:intergenic 45.0%
! TGGAAGGCATGGACATTGTT+AGG - chr1.2:86825666-86825685 MS.gene90909:intron 45.0%
!! ACTGGAGGCAAAAGTATCTC+TGG - chr1.2:86825222-86825241 MS.gene90909:CDS 45.0%
!! CCTAAAAGAAGCATCTCCGT+CGG - chr1.2:86824779-86824798 MS.gene90909:intron 45.0%
!! CTAAAAGAAGCATCTCCGTC+GGG - chr1.2:86824780-86824799 MS.gene90909:intron 45.0%
!! TAAAAGAAGCATCTCCGTCG+GGG - chr1.2:86824781-86824800 MS.gene90909:intron 45.0%
AATCCAGTTGGGAAGCTGGT+TGG - chr1.2:86824956-86824975 MS.gene90909:intron 50.0%
AATGCTGGTCCCAACACCAA+TGG - chr1.2:86825488-86825507 MS.gene90909:CDS 50.0%
ACAAGTGAAAGTGAGGGCTG+TGG - chr1.2:86824853-86824872 MS.gene90909:CDS 50.0%
ATGCTGGTCCCAACACCAAT+GGG - chr1.2:86825489-86825508 MS.gene90909:CDS 50.0%
CTAGTTTCACACCCACACCT+CGG + chr1.2:86824817-86824836 None:intergenic 50.0%
GCTGTTAGCATGGCAAATGC+TGG - chr1.2:86825473-86825492 MS.gene90909:CDS 50.0%
GTGGGTGTGAAACTAGCAGT+AGG - chr1.2:86824821-86824840 MS.gene90909:CDS 50.0%
TAGTTTCACACCCACACCTC+GGG + chr1.2:86824816-86824835 None:intergenic 50.0%
TATCTATCGCAGGGAACTGG+AGG - chr1.2:86825207-86825226 MS.gene90909:CDS 50.0%
TGGAAATCCAGTTGGGAAGC+TGG - chr1.2:86824952-86824971 MS.gene90909:intron 50.0%
TGGTGATCGTCCCCTAAAGA+AGG - chr1.2:86825715-86825734 MS.gene90909:intron 50.0%
TTTGCCATGCTAACAGCTCC+TGG + chr1.2:86825470-86825489 None:intergenic 50.0%
! CCGACGGAGATGCTTCTTTT+AGG + chr1.2:86824782-86824801 None:intergenic 50.0%
!! TGAAGTCCTTGATGACGCGA+TGG + chr1.2:86825087-86825106 None:intergenic 50.0%
!!! TGGCCAAGTTTTGGAAGGCA+TGG - chr1.2:86825655-86825674 MS.gene90909:intron 50.0%
AGCCCTTCTCACTGGTGGAA+AGG + chr1.2:86825042-86825061 None:intergenic 55.0%
CACCTTCCATCGCGTCATCA+AGG - chr1.2:86825078-86825097 MS.gene90909:CDS 55.0%
CAGTTGGGAAGCTGGTTGGA+AGG - chr1.2:86824960-86824979 MS.gene90909:intron 55.0%
CATGACATTGGCAGACGCTG+TGG - chr1.2:86825609-86825628 MS.gene90909:intron 55.0%
CATGCTAACAGCTCCTGGTC+CGG + chr1.2:86825465-86825484 None:intergenic 55.0%
CTTCCAACCAGCTTCCCAAC+TGG + chr1.2:86824962-86824981 None:intergenic 55.0%
GAATCACGGGAGACTGATCG+TGG - chr1.2:86825695-86825714 MS.gene90909:intron 55.0%
GCCCTTCTCACTGGTGGAAA+GGG + chr1.2:86825041-86825060 None:intergenic 55.0%
GCCCTTTCCACCAGTGAGAA+GGG - chr1.2:86825037-86825056 MS.gene90909:intron 55.0%
GTAGCCAAAGCCCTTCTCAC+TGG + chr1.2:86825050-86825069 None:intergenic 55.0%
TCCACCAGTGAGAAGGGCTT+TGG - chr1.2:86825043-86825062 MS.gene90909:intron 55.0%
TCTCACTGGTGGAAAGGGCA+CGG + chr1.2:86825036-86825055 None:intergenic 55.0%
TGCCCTTTCCACCAGTGAGA+AGG - chr1.2:86825036-86825055 MS.gene90909:intron 55.0%
!! CTTGATGACGCGATGGAAGG+TGG + chr1.2:86825080-86825099 None:intergenic 55.0%
!! GTCCTTGATGACGCGATGGA+AGG + chr1.2:86825083-86825102 None:intergenic 55.0%
ACATTGGCAGACGCTGTGGC+TGG - chr1.2:86825613-86825632 MS.gene90909:intron 60.0%
AGACGCTGTGGCTGGATCAG+AGG - chr1.2:86825621-86825640 MS.gene90909:intron 60.0%
CCAGTGTCTCATACCGGACC+AGG - chr1.2:86825449-86825468 MS.gene90909:CDS 60.0%
CCTGGTCCGGTATGAGACAC+TGG + chr1.2:86825452-86825471 None:intergenic 60.0%
CGGACCAGGAGCTGTTAGCA+TGG - chr1.2:86825463-86825482 MS.gene90909:CDS 60.0%
GATGACGCGATGGAAGGTGG+AGG + chr1.2:86825077-86825096 None:intergenic 60.0%
GCCAAAGCCCTTCTCACTGG+TGG + chr1.2:86825047-86825066 None:intergenic 60.0%
GTGGTTAGGTCCCGAGGTGT+GGG - chr1.2:86824803-86824822 MS.gene90909:CDS 60.0%
!! AAGAAGCATCTCCGTCGGGG+TGG - chr1.2:86824784-86824803 MS.gene90909:intron 60.0%
GCATCTCCGTCGGGGTGGTT+AGG - chr1.2:86824789-86824808 MS.gene90909:intron 65.0%
GGTGGTTAGGTCCCGAGGTG+TGG - chr1.2:86824802-86824821 MS.gene90909:CDS 65.0%
TCGGGACCTAACCACCCCGA+CGG + chr1.2:86824798-86824817 None:intergenic 65.0%
GTCGGGGTGGTTAGGTCCCG+AGG - chr1.2:86824797-86824816 MS.gene90909:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 86824559 86825775 86824559 ID=MS.gene90909
chr1.2 mRNA 86824559 86825775 86824559 ID=MS.gene90909.t1;Parent=MS.gene90909
chr1.2 exon 86825755 86825775 86825755 ID=MS.gene90909.t1.exon1;Parent=MS.gene90909.t1
chr1.2 CDS 86825755 86825775 86825755 ID=cds.MS.gene90909.t1;Parent=MS.gene90909.t1
chr1.2 exon 86825196 86825588 86825196 ID=MS.gene90909.t1.exon2;Parent=MS.gene90909.t1
chr1.2 CDS 86825196 86825588 86825196 ID=cds.MS.gene90909.t1;Parent=MS.gene90909.t1
chr1.2 exon 86825061 86825115 86825061 ID=MS.gene90909.t1.exon3;Parent=MS.gene90909.t1
chr1.2 CDS 86825061 86825115 86825061 ID=cds.MS.gene90909.t1;Parent=MS.gene90909.t1
chr1.2 exon 86824796 86824881 86824796 ID=MS.gene90909.t1.exon4;Parent=MS.gene90909.t1
chr1.2 CDS 86824796 86824881 86824796 ID=cds.MS.gene90909.t1;Parent=MS.gene90909.t1
chr1.2 exon 86824559 86824711 86824559 ID=MS.gene90909.t1.exon5;Parent=MS.gene90909.t1
chr1.2 CDS 86824559 86824711 86824559 ID=cds.MS.gene90909.t1;Parent=MS.gene90909.t1
Gene Sequence

>MS.gene90909

ATGGCTGTTGCTGCATTCAATGTTACTACAACACCGCCACTTTTCTCACTGATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTTCCATTACTTAAATTTAATTGATGTTGAATTCATTTTTATTAATTGATTGATTGTCTTGGTTGGGTGGTCAGTCAAATGTTGTTGCTCTGAATCCTAATCCTAACCCTAAAAGAAGCATCTCCGTCGGGGTGGTTAGGTCCCGAGGTGTGGGTGTGAAACTAGCAGTAGGAGTACGACGACAAGTGAAAGTGAGGGCTGTGGAAAATGGTAGTAGTTCAGAGGAGAAAGTAATAGTACAGTCAAAAGTGACTCACAAAGTATTCTTTGATGTTAGTATTGGAAATCCAGTTGGGAAGCTGGTTGGAAGGATTGTGATAGGACTCTTTGGTGATGATGTCCCGCAAACTGTTGAGAACTTCCGTGCCCTTTCCACCAGTGAGAAGGGCTTTGGCTACAAGTCCTCCACCTTCCATCGCGTCATCAAGGACTTCATGATTCAAGGAGGAGACTTTGATAAAGGAAATGTATGTATCTCTCTATCTATCGCTATCTCCTTCCTTTTTTTATTCATCAACTTATCTATCTATCTATCTATCTATCGCAGGGAACTGGAGGCAAAAGTATCTCTGGTCGTACTTTCAAAGATGAGAATTTTAATCGTGAGCTCTGTTTTCTTGCTTACTTATTCTTATTTCATTTCTACTGTCTTTGTTTCATGATACTGTCCCCCTTCACTGTGTTTATTTTATTTTAGTTTAGTTTAGTTTAGTTTAGGCTGCTAAATTATTAGTTCAAGCAATATTGCAAATATAATAATACTCGTGTACAATATCTCCAGTGTCTCATACCGGACCAGGAGCTGTTAGCATGGCAAATGCTGGTCCCAACACCAATGGGAGTCAGTTTTTCATATGCGCTGTCAAGGTATTTATTCTTCTATTCTCTCTTATCGGATGTTACTTTTTTGTTGACATAATAATAAACTTAATTGTCTCATGACATTGGCAGACGCTGTGGCTGGATCAGAGGCATGTTGTATTTGGCCAAGTTTTGGAAGGCATGGACATTGTTAGGTCGATTGAATCACGGGAGACTGATCGTGGTGATCGTCCCCTAAAGAAGGTGACAATCAGTGACTGTGGCGAGCTTCCAATTGCTTGA
Protein sequence

>MS.gene90909.t1

MAVAAFNSNVVALNPNPNPKRSISVGVVRSRGVGVKLAVGVRRQVKVRAVENGSSSEEKVIVQSKVTHKVFFDVSIGNPVGKLVGRIVIGLFGDDVPQTVENFRALSTSEKGFGYKSSTFHRVIKDFMIQGGDFDKGNGTGGKSISGRTFKDENFNLSHTGPGAVSMANAGPNTNGSQFFICAVKTLWLDQRHVVFGQVLEGMDIVRSIESRETDRGDRPLKKVTISDCGELPIA