Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90926.t1 | XP_013458946.1 | 93.6 | 173 | 11 | 0 | 1 | 173 | 1222 | 1394 | 4.60E-82 | 313.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90926.t1 | A0A072UVA4 | 93.6 | 173 | 11 | 0 | 1 | 173 | 1222 | 1394 | 3.3e-82 | 313.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene90926 | 0.81095 | 8.93E-51 | -1.69E-46 |
| MS.gene049873 | MS.gene90926 | 0.815174 | 1.06E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene90926 | -0.822331 | 2.51E-53 | -1.69E-46 |
| MS.gene05365 | MS.gene90926 | 0.800207 | 1.61E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene90926 | 0.80349 | 3.40E-49 | -1.69E-46 |
| MS.gene055374 | MS.gene90926 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene90926 | 0.802754 | 4.83E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene90926 | 0.801003 | 1.10E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene90926 | 0.805545 | 1.27E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene90926 | 0.807561 | 4.76E-50 | -1.69E-46 |
| MS.gene060879 | MS.gene90926 | 0.801815 | 7.53E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene90926 | -0.806968 | 6.36E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90926.t1 | MTR_3g012470 | 93.642 | 173 | 11 | 0 | 1 | 173 | 1222 | 1394 | 5.02e-105 | 328 |
| MS.gene90926.t1 | MTR_6g078420 | 38.393 | 112 | 69 | 0 | 1 | 112 | 1299 | 1410 | 9.49e-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATGAACAACCTTGTTCT+TGG | 0.283181 | 3.2:+6435265 | None:intergenic |
| AAGAACTAATAAATGGTGTT+AGG | 0.296835 | 3.2:-6435313 | MS.gene90926:CDS |
| GTGCAATGAGTTGGAATCTT+TGG | 0.312039 | 3.2:-6435357 | MS.gene90926:CDS |
| TTTCAAGACAATTCACTTGT+TGG | 0.342188 | 3.2:-6435514 | MS.gene90926:CDS |
| GAGCTAGAAGAACTAATAAA+TGG | 0.354948 | 3.2:-6435320 | MS.gene90926:CDS |
| TACATTTGTGAGGTAATCTA+AGG | 0.395126 | 3.2:+6435586 | None:intergenic |
| GGCAAATATTAGAGTTGAAT+GGG | 0.425634 | 3.2:-6435135 | MS.gene90926:CDS |
| AGGTTCAAAGAGATGAGTTA+AGG | 0.425882 | 3.2:-6435208 | MS.gene90926:CDS |
| GGTTCAAAGAGATGAGTTAA+GGG | 0.431237 | 3.2:-6435207 | MS.gene90926:CDS |
| ATTCAACTCTAATATTTGCC+TGG | 0.436699 | 3.2:+6435138 | None:intergenic |
| GTAATCTAAGGTAGATTTCA+TGG | 0.440572 | 3.2:+6435598 | None:intergenic |
| TTTAGCAAGGCTGCCAACTA+AGG | 0.440603 | 3.2:-6435095 | None:intergenic |
| AGAACTAATAAATGGTGTTA+GGG | 0.442237 | 3.2:-6435312 | MS.gene90926:CDS |
| TTTGAGAGATGAGCATGAAT+TGG | 0.445587 | 3.2:-6435171 | MS.gene90926:CDS |
| TGAGAATGCAAAGATTCTAA+AGG | 0.453615 | 3.2:-6435228 | MS.gene90926:CDS |
| AGGCAAATATTAGAGTTGAA+TGG | 0.453737 | 3.2:-6435136 | MS.gene90926:CDS |
| ATCTGTTGAAATAAGATCAA+TGG | 0.470245 | 3.2:-6435546 | MS.gene90926:CDS |
| AGTTATGATTACACAAATGA+AGG | 0.477628 | 3.2:-6435491 | MS.gene90926:CDS |
| ATCAACTAGACTGCCTTAGT+TGG | 0.483581 | 3.2:+6435082 | None:intergenic |
| GAAAAGTGATGAACTTGAAG+AGG | 0.513040 | 3.2:-6435435 | MS.gene90926:CDS |
| AAATGAGTTGTGCAATGAGT+TGG | 0.526689 | 3.2:-6435366 | MS.gene90926:CDS |
| ACAAATGTACCATCAGAAGA+TGG | 0.557757 | 3.2:-6435572 | MS.gene90926:CDS |
| ACAGATATTCCATCTTCTGA+TGG | 0.563310 | 3.2:+6435563 | None:intergenic |
| AGAGTTGAATGGGCAAAGCT+AGG | 0.564643 | 3.2:-6435125 | MS.gene90926:CDS |
| TGAATTGGCTAAGAAAATCC+AGG | 0.578112 | 3.2:-6435156 | MS.gene90926:CDS |
| CAATGAGTTGGAATCTTTGG+AGG | 0.579011 | 3.2:-6435354 | MS.gene90926:CDS |
| AAAAGTGATGAACTTGAAGA+GGG | 0.611601 | 3.2:-6435434 | MS.gene90926:CDS |
| TTCACAAGAAGCCAAGAACA+AGG | 0.623636 | 3.2:-6435276 | MS.gene90926:CDS |
| CTTCTGATGGTACATTTGTG+AGG | 0.626448 | 3.2:+6435576 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAACTAATAAATGGTGTT+AGG | - | chr3.2:6435380-6435399 | MS.gene90926:CDS | 25.0% |
| ! | AGTTATGATTACACAAATGA+AGG | - | chr3.2:6435202-6435221 | MS.gene90926:CDS | 25.0% |
| ! | ATCTGTTGAAATAAGATCAA+TGG | - | chr3.2:6435147-6435166 | MS.gene90926:CDS | 25.0% |
| !! | AGAACTAATAAATGGTGTTA+GGG | - | chr3.2:6435381-6435400 | MS.gene90926:CDS | 25.0% |
| !!! | TTCATCACTTTTCAACAATT+TGG | + | chr3.2:6435251-6435270 | None:intergenic | 25.0% |
| AAAAGTGATGAACTTGAAGA+GGG | - | chr3.2:6435259-6435278 | MS.gene90926:CDS | 30.0% | |
| AGGCAAATATTAGAGTTGAA+TGG | - | chr3.2:6435557-6435576 | MS.gene90926:CDS | 30.0% | |
| ATTCAACTCTAATATTTGCC+TGG | + | chr3.2:6435558-6435577 | None:intergenic | 30.0% | |
| GAGCTAGAAGAACTAATAAA+TGG | - | chr3.2:6435373-6435392 | MS.gene90926:CDS | 30.0% | |
| GGCAAATATTAGAGTTGAAT+GGG | - | chr3.2:6435558-6435577 | MS.gene90926:CDS | 30.0% | |
| TACATTTGTGAGGTAATCTA+AGG | + | chr3.2:6435110-6435129 | None:intergenic | 30.0% | |
| TGAGAATGCAAAGATTCTAA+AGG | - | chr3.2:6435465-6435484 | MS.gene90926:CDS | 30.0% | |
| TTTCAAGACAATTCACTTGT+TGG | - | chr3.2:6435179-6435198 | MS.gene90926:CDS | 30.0% | |
| ! | ATCTTTGGAGGAAAGAAAAA+AGG | - | chr3.2:6435351-6435370 | MS.gene90926:CDS | 30.0% |
| AAATGAGTTGTGCAATGAGT+TGG | - | chr3.2:6435327-6435346 | MS.gene90926:CDS | 35.0% | |
| ACAAATGTACCATCAGAAGA+TGG | - | chr3.2:6435121-6435140 | MS.gene90926:CDS | 35.0% | |
| ACAGATATTCCATCTTCTGA+TGG | + | chr3.2:6435133-6435152 | None:intergenic | 35.0% | |
| AGGTTCAAAGAGATGAGTTA+AGG | - | chr3.2:6435485-6435504 | MS.gene90926:CDS | 35.0% | |
| GAAAAGTGATGAACTTGAAG+AGG | - | chr3.2:6435258-6435277 | MS.gene90926:CDS | 35.0% | |
| GCTAGGTGAAAAATTTAGCA+AGG | - | chr3.2:6435585-6435604 | MS.gene90926:CDS | 35.0% | |
| GGTTCAAAGAGATGAGTTAA+GGG | - | chr3.2:6435486-6435505 | MS.gene90926:CDS | 35.0% | |
| TGAATTGGCTAAGAAAATCC+AGG | - | chr3.2:6435537-6435556 | MS.gene90926:CDS | 35.0% | |
| TGTATGAACAACCTTGTTCT+TGG | + | chr3.2:6435431-6435450 | None:intergenic | 35.0% | |
| TTTGAGAGATGAGCATGAAT+TGG | - | chr3.2:6435522-6435541 | MS.gene90926:CDS | 35.0% | |
| CTTCTGATGGTACATTTGTG+AGG | + | chr3.2:6435120-6435139 | None:intergenic | 40.0% | |
| TTCACAAGAAGCCAAGAACA+AGG | - | chr3.2:6435417-6435436 | MS.gene90926:CDS | 40.0% | |
| ! | CAATGAGTTGGAATCTTTGG+AGG | - | chr3.2:6435339-6435358 | MS.gene90926:CDS | 40.0% |
| ! | GTGCAATGAGTTGGAATCTT+TGG | - | chr3.2:6435336-6435355 | MS.gene90926:CDS | 40.0% |
| AGAGTTGAATGGGCAAAGCT+AGG | - | chr3.2:6435568-6435587 | MS.gene90926:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 6435097 | 6435618 | 6435097 | ID=MS.gene90926 |
| chr3.2 | mRNA | 6435097 | 6435618 | 6435097 | ID=MS.gene90926.t1;Parent=MS.gene90926 |
| chr3.2 | exon | 6435097 | 6435618 | 6435097 | ID=MS.gene90926.t1.exon1;Parent=MS.gene90926.t1 |
| chr3.2 | CDS | 6435097 | 6435618 | 6435097 | ID=cds.MS.gene90926.t1;Parent=MS.gene90926.t1 |
| Gene Sequence |
| Protein sequence |
