Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058635.t1 | XP_013457252.1 | 97.7 | 133 | 3 | 0 | 1 | 133 | 1 | 133 | 1.10E-62 | 249.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058635.t1 | C7AE94 | 70.8 | 130 | 38 | 0 | 3 | 132 | 7 | 136 | 4.5e-46 | 185.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058635.t1 | A0A072UQF3 | 97.7 | 133 | 3 | 0 | 1 | 133 | 1 | 133 | 7.7e-63 | 249.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene058635 | 0.805491 | 1.30E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene058635 | 0.804622 | 1.98E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene058635 | 0.800771 | 1.23E-48 | -1.69E-46 |
| MS.gene051832 | MS.gene058635 | 0.821325 | 4.29E-53 | -1.69E-46 |
| MS.gene053294 | MS.gene058635 | 0.808129 | 3.61E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene058635 | 0.807607 | 4.65E-50 | -1.69E-46 |
| MS.gene05795 | MS.gene058635 | 0.800321 | 1.52E-48 | -1.69E-46 |
| MS.gene058008 | MS.gene058635 | 0.805878 | 1.08E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene060840 | 0.812039 | 5.18E-51 | -1.69E-46 |
| MS.gene058635 | MS.gene061296 | 0.800246 | 1.58E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene061900 | 0.819286 | 1.26E-52 | -1.69E-46 |
| MS.gene058635 | MS.gene065949 | 0.822229 | 2.65E-53 | -1.69E-46 |
| MS.gene058635 | MS.gene066513 | 0.810919 | 9.07E-51 | -1.69E-46 |
| MS.gene058635 | MS.gene068385 | 0.802232 | 6.19E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene071442 | 0.801725 | 7.86E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene07815 | 0.804798 | 1.82E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene08361 | 0.849669 | 2.79E-60 | -1.69E-46 |
| MS.gene058635 | MS.gene09417 | 0.807117 | 5.91E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene09850 | 0.83297 | 6.97E-56 | -1.69E-46 |
| MS.gene058635 | MS.gene09930 | 0.801998 | 6.91E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene20392 | 0.80089 | 1.17E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene22557 | 0.80494 | 1.70E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene25350 | 0.808027 | 3.79E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene25686 | 0.847178 | 1.36E-59 | -1.69E-46 |
| MS.gene058635 | MS.gene25755 | 0.801206 | 1.00E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene25978 | 0.803541 | 3.32E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene26832 | 0.806013 | 1.01E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene27314 | 0.808794 | 2.60E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene28550 | 0.80411 | 2.53E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene28756 | 0.850485 | 1.65E-60 | -1.69E-46 |
| MS.gene058635 | MS.gene32801 | 0.804936 | 1.70E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene33131 | 0.814307 | 1.65E-51 | -1.69E-46 |
| MS.gene058635 | MS.gene34975 | 0.817395 | 3.37E-52 | -1.69E-46 |
| MS.gene058635 | MS.gene35069 | 0.923358 | 2.95E-89 | -1.69E-46 |
| MS.gene058635 | MS.gene38659 | 0.804559 | 2.04E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene38678 | -0.802696 | 4.96E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene40709 | 0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene40711 | 0.805566 | 1.25E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene44955 | 0.816551 | 5.22E-52 | -1.69E-46 |
| MS.gene058635 | MS.gene45334 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene47690 | 0.801434 | 9.02E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene47794 | 0.800654 | 1.30E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene48387 | 0.80789 | 4.05E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene53383 | 0.807501 | 4.90E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene54331 | 0.812004 | 5.27E-51 | -1.69E-46 |
| MS.gene058635 | MS.gene54883 | 0.826622 | 2.45E-54 | -1.69E-46 |
| MS.gene058635 | MS.gene54884 | 0.82295 | 1.80E-53 | -1.69E-46 |
| MS.gene058635 | MS.gene57128 | 0.825659 | 4.16E-54 | -1.69E-46 |
| MS.gene058635 | MS.gene58653 | 0.804007 | 2.66E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene59086 | 0.801463 | 8.90E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene59540 | 0.804611 | 1.99E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene61162 | 0.830246 | 3.27E-55 | -1.69E-46 |
| MS.gene058635 | MS.gene61288 | 0.811795 | 5.86E-51 | -1.69E-46 |
| MS.gene058635 | MS.gene61462 | 0.802508 | 5.43E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene62676 | 0.820655 | 6.12E-53 | -1.69E-46 |
| MS.gene058635 | MS.gene62677 | 0.813702 | 2.24E-51 | -1.69E-46 |
| MS.gene058635 | MS.gene64517 | 0.817355 | 3.44E-52 | -1.69E-46 |
| MS.gene058635 | MS.gene66474 | 0.801362 | 9.33E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene70515 | 0.829909 | 3.95E-55 | -1.69E-46 |
| MS.gene058635 | MS.gene71534 | 0.803215 | 3.88E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene71988 | 0.80115 | 1.03E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene72126 | 0.855027 | 8.31E-62 | -1.69E-46 |
| MS.gene058635 | MS.gene74517 | 0.843053 | 1.77E-58 | -1.69E-46 |
| MS.gene058635 | MS.gene74530 | 0.833126 | 6.38E-56 | -1.69E-46 |
| MS.gene058635 | MS.gene74758 | 0.801039 | 1.09E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene75147 | 0.860536 | 1.93E-63 | -1.69E-46 |
| MS.gene058635 | MS.gene76035 | 0.803297 | 3.73E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene76244 | 0.809208 | 2.12E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene78685 | 0.80611 | 9.64E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene79568 | 0.810287 | 1.24E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene79654 | 0.806996 | 6.27E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene87344 | 0.832111 | 1.14E-55 | -1.69E-46 |
| MS.gene058635 | MS.gene87377 | 0.800734 | 1.25E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene87673 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene87969 | 0.806472 | 8.09E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene88351 | 0.805612 | 1.23E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene90862 | 0.828999 | 6.58E-55 | -1.69E-46 |
| MS.gene058635 | MS.gene90926 | 0.805545 | 1.27E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene95655 | 0.801196 | 1.01E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058635.t1 | MTR_4g094368 | 97.744 | 133 | 3 | 0 | 1 | 133 | 1 | 133 | 3.45e-90 | 261 |
| MS.gene058635.t1 | MTR_4g085590 | 58.537 | 123 | 49 | 1 | 3 | 125 | 21 | 141 | 1.36e-45 | 148 |
| MS.gene058635.t1 | MTR_4g094812 | 62.393 | 117 | 42 | 1 | 3 | 119 | 20 | 134 | 2.79e-44 | 145 |
| MS.gene058635.t1 | MTR_4g094925 | 60.163 | 123 | 43 | 2 | 5 | 125 | 19 | 137 | 1.16e-43 | 143 |
| MS.gene058635.t1 | MTR_4g094898 | 60.684 | 117 | 44 | 2 | 3 | 119 | 22 | 136 | 1.06e-42 | 141 |
| MS.gene058635.t1 | MTR_4g094815 | 55.385 | 130 | 56 | 2 | 3 | 132 | 22 | 149 | 1.50e-42 | 140 |
| MS.gene058635.t1 | MTR_8g021225 | 56.198 | 121 | 51 | 1 | 5 | 125 | 11 | 129 | 5.00e-40 | 134 |
| MS.gene058635.t1 | MTR_8g101900 | 53.488 | 129 | 58 | 1 | 5 | 133 | 12 | 138 | 1.54e-33 | 117 |
| MS.gene058635.t1 | MTR_4g094925 | 62.195 | 82 | 31 | 0 | 44 | 125 | 25 | 106 | 4.83e-31 | 110 |
| MS.gene058635.t1 | MTR_4g094898 | 68.000 | 75 | 24 | 0 | 45 | 119 | 1 | 75 | 8.60e-31 | 108 |
| MS.gene058635.t1 | MTR_6g091680 | 50.781 | 128 | 55 | 3 | 4 | 125 | 8 | 133 | 1.26e-28 | 104 |
| MS.gene058635.t1 | MTR_2g070410 | 37.600 | 125 | 70 | 3 | 8 | 129 | 88 | 207 | 4.59e-21 | 85.9 |
| MS.gene058635.t1 | MTR_2g070410 | 37.600 | 125 | 70 | 3 | 8 | 129 | 88 | 207 | 4.97e-21 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058635.t1 | AT1G67980 | 67.176 | 131 | 43 | 0 | 3 | 133 | 8 | 138 | 3.56e-61 | 187 |
| MS.gene058635.t1 | AT1G24735 | 67.969 | 128 | 41 | 0 | 5 | 132 | 16 | 143 | 1.12e-59 | 184 |
| MS.gene058635.t1 | AT1G67990 | 61.069 | 131 | 51 | 0 | 3 | 133 | 8 | 138 | 3.60e-55 | 172 |
| MS.gene058635.t1 | AT1G24735 | 48.603 | 179 | 41 | 1 | 5 | 132 | 16 | 194 | 6.37e-50 | 160 |
| MS.gene058635.t1 | AT4G34050 | 56.098 | 123 | 52 | 1 | 3 | 125 | 33 | 153 | 1.10e-42 | 141 |
| MS.gene058635.t1 | AT4G34050 | 56.098 | 123 | 52 | 1 | 3 | 125 | 60 | 180 | 2.16e-42 | 141 |
| MS.gene058635.t1 | AT4G26220 | 53.030 | 132 | 60 | 2 | 2 | 133 | 6 | 135 | 9.68e-42 | 138 |
| MS.gene058635.t1 | AT1G24735 | 67.391 | 92 | 30 | 0 | 41 | 132 | 7 | 98 | 2.24e-39 | 131 |
| MS.gene058635.t1 | AT3G61990 | 40.800 | 125 | 66 | 2 | 8 | 132 | 83 | 199 | 2.39e-24 | 94.7 |
| MS.gene058635.t1 | AT3G62000 | 36.800 | 125 | 74 | 2 | 8 | 132 | 68 | 187 | 2.71e-19 | 81.3 |
| MS.gene058635.t1 | AT3G62000 | 36.800 | 125 | 74 | 2 | 8 | 132 | 68 | 187 | 6.11e-19 | 81.3 |
| MS.gene058635.t1 | AT1G67980 | 46.377 | 69 | 37 | 0 | 65 | 133 | 2 | 70 | 6.79e-13 | 62.4 |
Find 20 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACAACAATTCAGAAGTA+TGG | 0.226874 | 4.2:+18433700 | MS.gene058635:CDS |
| TTCAATTGTTCGTGCTCTTT+TGG | 0.241069 | 4.2:-18433667 | None:intergenic |
| TTTCATCACTTTCAGGTAAT+AGG | 0.252449 | 4.2:+18434084 | MS.gene058635:intron |
| ATCCACAGTGATGCATTATC+AGG | 0.304543 | 4.2:+18434186 | MS.gene058635:CDS |
| TCAGGTAATAGGAATTGATA+TGG | 0.352155 | 4.2:+18434095 | MS.gene058635:intron |
| CAAAACTCCAGCACTTCTTC+AGG | 0.360798 | 4.2:+18433521 | MS.gene058635:CDS |
| TTTGAGGATGTTCTTGGCCA+TGG | 0.424703 | 4.2:-18433502 | None:intergenic |
| AACTTCTGCCTTCTGAATGA+AGG | 0.443554 | 4.2:-18434145 | None:intergenic |
| TGCTCTTCCTTCTGATGGCA+AGG | 0.457076 | 4.2:+18433995 | MS.gene058635:CDS |
| GCTCTTGCTCTTCCTTCTGA+TGG | 0.470052 | 4.2:+18433990 | MS.gene058635:CDS |
| TTAGAAATTGGAGTCTTCAC+TGG | 0.475666 | 4.2:+18433948 | MS.gene058635:CDS |
| ATTATTACCTGAAGAAGTGC+TGG | 0.476254 | 4.2:-18433528 | None:intergenic |
| GAGTATAATGAATGTGCCTT+TGG | 0.477530 | 4.2:+18433872 | MS.gene058635:CDS |
| AAACAACAATTCAGAAGTAT+GGG | 0.513999 | 4.2:+18433701 | MS.gene058635:CDS |
| GATAAAGAAGCATATGAGAC+AGG | 0.521908 | 4.2:+18434117 | MS.gene058635:CDS |
| AATGATACCTTGCCATCAGA+AGG | 0.554204 | 4.2:-18434002 | None:intergenic |
| ACTTCTGCCTTCTGAATGAA+GGG | 0.557570 | 4.2:-18434144 | None:intergenic |
| ATGAATTGTGCTTCATCCAA+AGG | 0.563739 | 4.2:-18433888 | None:intergenic |
| AGGATTGCCCTTCATTCAGA+AGG | 0.568251 | 4.2:+18434137 | MS.gene058635:CDS |
| TGCCTGATAATGCATCACTG+TGG | 0.800721 | 4.2:-18434188 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATTGATTTTAATATTTGT+TGG | + | chr4.2:18433834-18433853 | MS.gene058635:intron | 15.0% |
| !!! | CATTGATTTTAATATTTGTT+GGG | + | chr4.2:18433835-18433854 | MS.gene058635:intron | 15.0% |
| !! | TAATTAATATCTCTAGTAGA+AGG | - | chr4.2:18433596-18433615 | None:intergenic | 20.0% |
| !! | TTAAGAAAACTCAATTTATC+TGG | - | chr4.2:18433572-18433591 | None:intergenic | 20.0% |
| ! | GAATAAATTTCATCACTTTC+AGG | + | chr4.2:18434077-18434096 | MS.gene058635:intron | 25.0% |
| ! | GCGAAAAAAACATTAGAAAT+TGG | + | chr4.2:18433936-18433955 | MS.gene058635:CDS | 25.0% |
| !! | AAACAACAATTCAGAAGTAT+GGG | + | chr4.2:18433701-18433720 | MS.gene058635:CDS | 25.0% |
| !! | AACTTGTTTCAAGAATATAC+TGG | - | chr4.2:18433642-18433661 | None:intergenic | 25.0% |
| !!! | ATTTTAATATTTGTTGGGTG+TGG | + | chr4.2:18433840-18433859 | MS.gene058635:intron | 25.0% |
| GAAACAACAATTCAGAAGTA+TGG | + | chr4.2:18433700-18433719 | MS.gene058635:CDS | 30.0% | |
| TCAGGTAATAGGAATTGATA+TGG | + | chr4.2:18434095-18434114 | MS.gene058635:intron | 30.0% | |
| ! | TTTCATCACTTTCAGGTAAT+AGG | + | chr4.2:18434084-18434103 | MS.gene058635:intron | 30.0% |
| ATGAATTGTGCTTCATCCAA+AGG | - | chr4.2:18433891-18433910 | None:intergenic | 35.0% | |
| GAGTATAATGAATGTGCCTT+TGG | + | chr4.2:18433872-18433891 | MS.gene058635:CDS | 35.0% | |
| TTAGAAATTGGAGTCTTCAC+TGG | + | chr4.2:18433948-18433967 | MS.gene058635:CDS | 35.0% | |
| ! | ATTATTACCTGAAGAAGTGC+TGG | - | chr4.2:18433531-18433550 | None:intergenic | 35.0% |
| ! | CTCAATTTATCTGGTTTCAG+TGG | - | chr4.2:18433563-18433582 | None:intergenic | 35.0% |
| ! | GATAAAGAAGCATATGAGAC+AGG | + | chr4.2:18434117-18434136 | MS.gene058635:CDS | 35.0% |
| ! | TTCAATTGTTCGTGCTCTTT+TGG | - | chr4.2:18433670-18433689 | None:intergenic | 35.0% |
| AACTTCTGCCTTCTGAATGA+AGG | - | chr4.2:18434148-18434167 | None:intergenic | 40.0% | |
| AATGATACCTTGCCATCAGA+AGG | - | chr4.2:18434005-18434024 | None:intergenic | 40.0% | |
| ACTTCTGCCTTCTGAATGAA+GGG | - | chr4.2:18434147-18434166 | None:intergenic | 40.0% | |
| ATCCACAGTGATGCATTATC+AGG | + | chr4.2:18434186-18434205 | MS.gene058635:CDS | 40.0% | |
| ! | TTGTTCGTGCTCTTTTGGAT+AGG | - | chr4.2:18433665-18433684 | None:intergenic | 40.0% |
| !!! | TGGAGTTTTGAGGATGTTCT+TGG | - | chr4.2:18433511-18433530 | None:intergenic | 40.0% |
| AGGATTGCCCTTCATTCAGA+AGG | + | chr4.2:18434137-18434156 | MS.gene058635:CDS | 45.0% | |
| CAAAACTCCAGCACTTCTTC+AGG | + | chr4.2:18433521-18433540 | MS.gene058635:CDS | 45.0% | |
| ! | ATATTTGTTGGGTGTGGCAC+TGG | + | chr4.2:18433846-18433865 | MS.gene058635:intron | 45.0% |
| ! | TGCCTGATAATGCATCACTG+TGG | - | chr4.2:18434191-18434210 | None:intergenic | 45.0% |
| !!! | GAAGAAGTGCTGGAGTTTTG+AGG | - | chr4.2:18433521-18433540 | None:intergenic | 45.0% |
| GCTCTTGCTCTTCCTTCTGA+TGG | + | chr4.2:18433990-18434009 | MS.gene058635:CDS | 50.0% | |
| TGCTCTTCCTTCTGATGGCA+AGG | + | chr4.2:18433995-18434014 | MS.gene058635:CDS | 50.0% | |
| !! | TTGTTGGGTGTGGCACTGGC+AGG | + | chr4.2:18433850-18433869 | MS.gene058635:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 18433504 | 18434227 | 18433504 | ID=MS.gene058635 |
| chr4.2 | mRNA | 18433504 | 18434227 | 18433504 | ID=MS.gene058635.t1;Parent=MS.gene058635 |
| chr4.2 | exon | 18433504 | 18433542 | 18433504 | ID=MS.gene058635.t1.exon1;Parent=MS.gene058635.t1 |
| chr4.2 | CDS | 18433504 | 18433542 | 18433504 | ID=cds.MS.gene058635.t1;Parent=MS.gene058635.t1 |
| chr4.2 | exon | 18433643 | 18433728 | 18433643 | ID=MS.gene058635.t1.exon2;Parent=MS.gene058635.t1 |
| chr4.2 | CDS | 18433643 | 18433728 | 18433643 | ID=cds.MS.gene058635.t1;Parent=MS.gene058635.t1 |
| chr4.2 | exon | 18433872 | 18434016 | 18433872 | ID=MS.gene058635.t1.exon3;Parent=MS.gene058635.t1 |
| chr4.2 | CDS | 18433872 | 18434016 | 18433872 | ID=cds.MS.gene058635.t1;Parent=MS.gene058635.t1 |
| chr4.2 | exon | 18434099 | 18434227 | 18434099 | ID=MS.gene058635.t1.exon4;Parent=MS.gene058635.t1 |
| chr4.2 | CDS | 18434099 | 18434227 | 18434099 | ID=cds.MS.gene058635.t1;Parent=MS.gene058635.t1 |
| Gene Sequence |
| Protein sequence |
