Alfalfa Gene Editing Database
Nr:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene91236.t1 | XP_003615966.1 | 93.3 | 360 | 24 | 0 | 1 | 360 | 1 | 360 | 3.20E-194 | 687.6 |
Swissprot:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene91236.t1 | B0ZB57 | 62.8 | 360 | 128 | 3 | 6 | 360 | 19 | 377 | 1.9e-131 | 470.3 |
Trembl:
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene91236.t1 | G7KFJ3 | 93.3 | 360 | 24 | 0 | 1 | 360 | 1 | 360 | 2.3e-194 | 687.6 |
TFs/TRs:
Gene ID | Type | Classification |
---|
Protein Kinases:
Gene ID | Type | Classification |
---|
Network:
Co-expression Network:
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MS.gene04932 | MS.gene91236 | 0.803298 | 3.73E-49 | -1.69E-46 |
MS.gene050326 | MS.gene91236 | 0.805851 | 1.09E-49 | -1.69E-46 |
MS.gene050328 | MS.gene91236 | 0.81227 | 4.61E-51 | -1.69E-46 |
MS.gene053097 | MS.gene91236 | 0.800866 | 1.18E-48 | -1.69E-46 |
MS.gene056198 | MS.gene91236 | 0.804142 | 2.49E-49 | -1.69E-46 |
MS.gene056694 | MS.gene91236 | 0.84279 | 2.08E-58 | -1.69E-46 |
MS.gene056697 | MS.gene91236 | 0.818601 | 1.80E-52 | -1.69E-46 |
MS.gene058382 | MS.gene91236 | 0.833848 | 4.21E-56 | -1.69E-46 |
MS.gene05926 | MS.gene91236 | 0.812928 | 3.31E-51 | -1.69E-46 |
MS.gene060405 | MS.gene91236 | 0.801512 | 8.70E-49 | -1.69E-46 |
MS.gene061044 | MS.gene91236 | 0.828252 | 9.97E-55 | -1.69E-46 |
PPI:
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene91236.t1 | MTR_5g074580 | 93.333 | 360 | 24 | 0 | 1 | 360 | 1 | 360 | 0.0 | 697 |
MS.gene91236.t1 | MTR_5g074680 | 84.401 | 359 | 56 | 0 | 1 | 359 | 1 | 359 | 0.0 | 639 |
MS.gene91236.t1 | MTR_5g074600 | 82.222 | 360 | 63 | 1 | 1 | 360 | 1 | 359 | 0.0 | 618 |
MS.gene91236.t1 | MTR_5g074540 | 76.111 | 360 | 67 | 2 | 1 | 360 | 1 | 341 | 0.0 | 565 |
MS.gene91236.t1 | MTR_5g074610 | 73.889 | 360 | 60 | 3 | 1 | 360 | 1 | 326 | 0.0 | 543 |
MS.gene91236.t1 | MTR_4g079160 | 63.989 | 361 | 123 | 5 | 1 | 360 | 1 | 355 | 1.97e-169 | 476 |
MS.gene91236.t1 | MTR_4g033200 | 49.727 | 366 | 175 | 6 | 1 | 360 | 1 | 363 | 2.87e-117 | 344 |
MS.gene91236.t1 | MTR_7g014570 | 48.424 | 349 | 173 | 4 | 12 | 360 | 11 | 352 | 6.38e-117 | 343 |
MS.gene91236.t1 | MTR_4g033015 | 49.454 | 366 | 176 | 6 | 1 | 360 | 1 | 363 | 5.67e-116 | 341 |
MS.gene91236.t1 | MTR_4g088200 | 46.742 | 353 | 182 | 5 | 12 | 360 | 11 | 361 | 3.53e-115 | 339 |
MS.gene91236.t1 | MTR_1g076940 | 46.027 | 365 | 188 | 7 | 1 | 360 | 1 | 361 | 9.29e-113 | 333 |
MS.gene91236.t1 | MTR_4g033150 | 48.626 | 364 | 176 | 6 | 3 | 360 | 5 | 363 | 1.48e-112 | 332 |
MS.gene91236.t1 | MTR_7g014510 | 46.307 | 352 | 181 | 5 | 12 | 360 | 11 | 357 | 5.53e-112 | 330 |
MS.gene91236.t1 | MTR_4g088190 | 47.753 | 356 | 177 | 5 | 12 | 360 | 11 | 364 | 5.25e-109 | 323 |
MS.gene91236.t1 | MTR_7g024520 | 45.531 | 358 | 185 | 5 | 1 | 358 | 1 | 348 | 6.27e-108 | 320 |
MS.gene91236.t1 | MTR_7g024500 | 44.413 | 358 | 189 | 5 | 1 | 358 | 1 | 348 | 7.14e-108 | 320 |
MS.gene91236.t1 | MTR_4g088345 | 45.682 | 359 | 190 | 4 | 3 | 360 | 2 | 356 | 2.10e-107 | 319 |
MS.gene91236.t1 | MTR_4g033170 | 45.730 | 363 | 186 | 6 | 1 | 360 | 3 | 357 | 1.84e-98 | 296 |
MS.gene91236.t1 | MTR_4g088340 | 45.104 | 337 | 180 | 4 | 25 | 360 | 1 | 333 | 3.46e-98 | 295 |
MS.gene91236.t1 | MTR_4g033085 | 44.353 | 363 | 191 | 6 | 1 | 360 | 3 | 357 | 2.50e-94 | 285 |
MS.gene91236.t1 | MTR_3g083620 | 37.389 | 337 | 204 | 4 | 21 | 356 | 27 | 357 | 3.27e-77 | 242 |
MS.gene91236.t1 | MTR_4g038440 | 39.429 | 350 | 202 | 7 | 15 | 360 | 25 | 368 | 1.27e-76 | 240 |
MS.gene91236.t1 | MTR_0079s0070 | 39.645 | 338 | 194 | 7 | 27 | 360 | 3 | 334 | 4.69e-74 | 233 |
MS.gene91236.t1 | MTR_0017s0100 | 38.000 | 350 | 207 | 6 | 15 | 360 | 25 | 368 | 5.68e-70 | 223 |
MS.gene91236.t1 | MTR_0794s0020 | 42.570 | 249 | 133 | 5 | 3 | 245 | 5 | 249 | 8.43e-65 | 206 |
MS.gene91236.t1 | MTR_1g076930 | 41.597 | 238 | 135 | 3 | 25 | 259 | 1 | 237 | 2.88e-60 | 194 |
MS.gene91236.t1 | MTR_7g118300 | 32.424 | 330 | 209 | 9 | 32 | 355 | 47 | 368 | 4.75e-47 | 164 |
MS.gene91236.t1 | MTR_4g038440 | 36.694 | 248 | 152 | 3 | 15 | 259 | 25 | 270 | 1.18e-45 | 157 |
MS.gene91236.t1 | MTR_0591s0010 | 30.816 | 331 | 190 | 7 | 32 | 341 | 46 | 358 | 2.02e-45 | 160 |
MS.gene91236.t1 | MTR_7g118320 | 30.675 | 326 | 207 | 10 | 31 | 345 | 30 | 347 | 5.67e-45 | 158 |
MS.gene91236.t1 | MTR_3g092900 | 30.247 | 324 | 209 | 9 | 32 | 346 | 34 | 349 | 6.46e-45 | 158 |
MS.gene91236.t1 | MTR_8g068870 | 28.852 | 357 | 195 | 8 | 20 | 345 | 20 | 348 | 2.44e-44 | 156 |
MS.gene91236.t1 | MTR_5g098170 | 29.969 | 327 | 209 | 9 | 32 | 347 | 33 | 350 | 2.99e-44 | 156 |
MS.gene91236.t1 | MTR_8g068750 | 29.070 | 344 | 187 | 6 | 32 | 345 | 46 | 362 | 3.81e-44 | 156 |
MS.gene91236.t1 | MTR_8g068690 | 30.503 | 318 | 208 | 7 | 32 | 341 | 45 | 357 | 4.52e-42 | 150 |
MS.gene91236.t1 | MTR_8g024160 | 34.409 | 279 | 166 | 8 | 86 | 359 | 61 | 327 | 5.20e-42 | 149 |
MS.gene91236.t1 | MTR_1g096310 | 28.313 | 332 | 220 | 7 | 27 | 347 | 34 | 358 | 9.09e-42 | 150 |
MS.gene91236.t1 | MTR_1g036490 | 31.070 | 383 | 210 | 13 | 5 | 359 | 13 | 369 | 1.18e-40 | 147 |
MS.gene91236.t1 | MTR_6g078850 | 30.275 | 327 | 207 | 10 | 32 | 345 | 50 | 368 | 1.47e-40 | 149 |
MS.gene91236.t1 | MTR_1g036460 | 29.921 | 381 | 233 | 11 | 1 | 359 | 1 | 369 | 5.80e-40 | 145 |
MS.gene91236.t1 | MTR_7g012090 | 28.531 | 354 | 214 | 11 | 27 | 355 | 34 | 373 | 6.56e-40 | 145 |
MS.gene91236.t1 | MTR_3g021430 | 29.972 | 357 | 224 | 11 | 1 | 346 | 1 | 342 | 1.32e-39 | 144 |
MS.gene91236.t1 | MTR_7g012070 | 29.392 | 296 | 184 | 8 | 74 | 355 | 89 | 373 | 3.71e-39 | 143 |
MS.gene91236.t1 | MTR_7g012040 | 28.446 | 341 | 225 | 9 | 27 | 355 | 33 | 366 | 4.94e-39 | 142 |
MS.gene91236.t1 | MTR_2g055940 | 30.120 | 249 | 159 | 6 | 115 | 359 | 125 | 362 | 3.27e-38 | 140 |
MS.gene91236.t1 | MTR_3g021440 | 30.938 | 320 | 209 | 8 | 34 | 347 | 43 | 356 | 2.58e-37 | 138 |
MS.gene91236.t1 | MTR_7g011900 | 27.356 | 329 | 225 | 8 | 27 | 347 | 34 | 356 | 1.43e-36 | 136 |
MS.gene91236.t1 | MTR_1g036510 | 36.816 | 201 | 122 | 3 | 147 | 347 | 174 | 369 | 4.24e-36 | 135 |
MS.gene91236.t1 | MTR_7g011990 | 29.388 | 245 | 167 | 4 | 116 | 359 | 127 | 366 | 6.70e-36 | 134 |
MS.gene91236.t1 | MTR_7g012020 | 26.531 | 343 | 235 | 9 | 27 | 359 | 33 | 368 | 2.47e-35 | 132 |
MS.gene91236.t1 | MTR_2g072790 | 30.218 | 321 | 209 | 8 | 32 | 345 | 27 | 339 | 1.88e-34 | 130 |
MS.gene91236.t1 | MTR_8g068570 | 38.462 | 169 | 94 | 5 | 162 | 327 | 156 | 317 | 3.60e-32 | 123 |
MS.gene91236.t1 | MTR_8g068730 | 26.163 | 344 | 165 | 6 | 32 | 345 | 46 | 330 | 2.18e-31 | 121 |
MS.gene91236.t1 | MTR_3g096050 | 28.000 | 350 | 232 | 12 | 8 | 345 | 2 | 343 | 2.81e-30 | 119 |
MS.gene91236.t1 | MTR_3g096070 | 27.920 | 351 | 208 | 13 | 8 | 345 | 2 | 320 | 6.97e-29 | 114 |
MS.gene91236.t1 | MTR_8g068600 | 26.809 | 235 | 150 | 7 | 40 | 260 | 28 | 254 | 2.29e-22 | 95.9 |
MS.gene91236.t1 | MTR_1g050220 | 33.758 | 157 | 90 | 3 | 194 | 350 | 151 | 293 | 1.47e-21 | 94.0 |
MS.gene91236.t1 | MTR_8g068620 | 37.168 | 113 | 70 | 1 | 150 | 262 | 2 | 113 | 5.04e-21 | 87.4 |
MS.gene91236.t1 | MTR_7g012020 | 25.820 | 244 | 173 | 5 | 27 | 263 | 33 | 275 | 5.32e-21 | 92.4 |
MS.gene91236.t1 | MTR_8g068690 | 26.360 | 239 | 166 | 6 | 32 | 262 | 45 | 281 | 7.53e-21 | 91.7 |
MS.gene91236.t1 | MTR_8g068740 | 23.958 | 288 | 133 | 5 | 32 | 289 | 46 | 277 | 5.52e-19 | 86.3 |
MS.gene91236.t1 | MTR_3g096050 | 27.481 | 262 | 176 | 9 | 8 | 259 | 2 | 259 | 4.15e-18 | 83.6 |
MS.gene91236.t1 | MTR_1g036510 | 33.628 | 113 | 74 | 1 | 147 | 259 | 174 | 285 | 5.19e-15 | 75.1 |
MS.gene91236.t1 | MTR_4g025570 | 42.268 | 97 | 51 | 4 | 261 | 356 | 122 | 214 | 1.19e-13 | 69.7 |
MS.gene91236.t1 | MTR_7g012020 | 25.000 | 212 | 151 | 5 | 27 | 231 | 33 | 243 | 1.26e-13 | 70.5 |
MS.gene91236.t1 | MTR_8g068760 | 40.230 | 87 | 49 | 1 | 259 | 345 | 135 | 218 | 5.91e-13 | 68.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MS.gene91236.t1 | AT4G35160 | 34.771 | 371 | 221 | 9 | 4 | 359 | 16 | 380 | 5.32e-66 | 214 |
MS.gene91236.t1 | AT4G35150 | 33.243 | 367 | 189 | 9 | 4 | 359 | 2 | 323 | 1.64e-60 | 197 |
MS.gene91236.t1 | AT1G51990 | 33.553 | 304 | 190 | 7 | 63 | 360 | 65 | 362 | 5.25e-48 | 166 |
MS.gene91236.t1 | AT1G51990 | 33.553 | 304 | 190 | 7 | 63 | 360 | 65 | 362 | 6.49e-48 | 166 |
MS.gene91236.t1 | AT5G54160 | 31.562 | 320 | 208 | 8 | 32 | 346 | 34 | 347 | 1.80e-47 | 165 |
MS.gene91236.t1 | AT1G33030 | 33.231 | 325 | 198 | 12 | 32 | 346 | 21 | 336 | 2.70e-42 | 151 |
MS.gene91236.t1 | AT1G77520 | 28.418 | 373 | 240 | 10 | 2 | 359 | 20 | 380 | 1.15e-40 | 147 |
MS.gene91236.t1 | AT3G53140 | 30.887 | 327 | 204 | 10 | 32 | 345 | 27 | 344 | 3.32e-39 | 143 |
MS.gene91236.t1 | AT5G53810 | 31.897 | 348 | 211 | 13 | 30 | 359 | 38 | 377 | 1.80e-36 | 136 |
MS.gene91236.t1 | AT1G63140 | 29.491 | 373 | 236 | 11 | 2 | 359 | 20 | 380 | 8.71e-34 | 129 |
MS.gene91236.t1 | AT1G63140 | 29.491 | 373 | 236 | 11 | 2 | 359 | 40 | 400 | 1.05e-33 | 129 |
MS.gene91236.t1 | AT1G21130 | 29.429 | 333 | 204 | 12 | 32 | 346 | 40 | 359 | 1.67e-33 | 128 |
MS.gene91236.t1 | AT1G77530 | 30.027 | 373 | 234 | 11 | 2 | 359 | 20 | 380 | 2.08e-33 | 128 |
MS.gene91236.t1 | AT1G21110 | 28.267 | 329 | 205 | 12 | 32 | 342 | 40 | 355 | 2.29e-30 | 119 |
MS.gene91236.t1 | AT5G37170 | 33.803 | 213 | 129 | 7 | 151 | 359 | 29 | 233 | 2.62e-30 | 116 |
MS.gene91236.t1 | AT1G21100 | 27.928 | 333 | 209 | 12 | 32 | 346 | 40 | 359 | 5.04e-30 | 118 |
MS.gene91236.t1 | AT1G21120 | 28.228 | 333 | 208 | 12 | 32 | 346 | 40 | 359 | 5.58e-30 | 118 |
MS.gene91236.t1 | AT5G37170 | 33.803 | 213 | 129 | 7 | 151 | 359 | 129 | 333 | 8.06e-30 | 117 |
MS.gene91236.t1 | AT1G21120 | 28.228 | 333 | 208 | 12 | 32 | 346 | 88 | 407 | 1.23e-29 | 118 |
MS.gene91236.t1 | AT1G62900 | 35.577 | 208 | 129 | 3 | 153 | 359 | 1 | 204 | 3.05e-29 | 112 |
MS.gene91236.t1 | AT1G76790 | 27.190 | 331 | 206 | 10 | 32 | 341 | 30 | 346 | 1.83e-28 | 114 |
MS.gene91236.t1 | AT5G37170 | 34.946 | 186 | 109 | 6 | 181 | 359 | 5 | 185 | 8.90e-27 | 105 |
MS.gene91236.t1 | AT1G33030 | 29.661 | 236 | 154 | 8 | 32 | 259 | 21 | 252 | 6.66e-20 | 88.6 |
MS.gene91236.t1 | AT1G33030 | 29.661 | 236 | 154 | 8 | 32 | 259 | 21 | 252 | 9.80e-20 | 88.2 |
MS.gene91236.t1 | AT1G63140 | 26.471 | 272 | 177 | 9 | 2 | 259 | 40 | 302 | 1.67e-14 | 73.9 |
MS.gene91236.t1 | AT1G21130 | 27.160 | 243 | 154 | 8 | 32 | 259 | 40 | 274 | 2.40e-12 | 67.4 |
Find 68 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
CAACATTCTCTTCATTATTT+TGG | 0.038167 | 5.1:-57249045 | None:intergenic |
CTTAAATGTGTTGTTGATTT+AGG | 0.154523 | 5.1:+57249136 | MS.gene91236:CDS |
CGACACACGGGATGTTGATA+TGG | 0.233179 | 5.1:+57249482 | MS.gene91236:CDS |
AGAGACCTTAAGCCTAAAAT+TGG | 0.252210 | 5.1:-57250184 | None:intergenic |
CCATAATTGTGTATGATATC+AGG | 0.254841 | 5.1:-57249163 | None:intergenic |
CCTGATATCATACACAATTA+TGG | 0.284388 | 5.1:+57249163 | MS.gene91236:CDS |
ATGCGAATAATGACTCATTC+TGG | 0.312409 | 5.1:+57249262 | MS.gene91236:CDS |
CGTGGCATCAATTCTCTACT+TGG | 0.365039 | 5.1:+57249428 | MS.gene91236:CDS |
GCTTTGAGCTTTGAGTAAAT+TGG | 0.373576 | 5.1:-57249071 | None:intergenic |
TGCAGTGGATATTGCATGAT+TGG | 0.384572 | 5.1:+57249915 | MS.gene91236:intron |
TGCCACGGGTTTGTCAAAAC+TGG | 0.395490 | 5.1:-57249412 | None:intergenic |
ATGGATTAAGTTGATTTCTT+CGG | 0.409901 | 5.1:+57250135 | MS.gene91236:CDS |
TGCTAAATCATTCCCACAAA+TGG | 0.411413 | 5.1:+57249672 | MS.gene91236:CDS |
ATTCAATGGATTGGAGTCAT+TGG | 0.421743 | 5.1:+57249609 | MS.gene91236:CDS |
TCAAAGCTCAAAGCCACATA+TGG | 0.423149 | 5.1:+57249080 | MS.gene91236:CDS |
AAGAGAGAAACGAGAAAGAA+TGG | 0.424421 | 5.1:+57250116 | MS.gene91236:CDS |
TGCATCCGTATTACTACTTA+AGG | 0.425584 | 5.1:+57249345 | MS.gene91236:CDS |
TTGGAGTCATTGGTTGATGT+TGG | 0.427653 | 5.1:+57249619 | MS.gene91236:CDS |
AACAAAATGGCGTCTGTTGG+AGG | 0.433773 | 5.1:-57249787 | None:intergenic |
TTTGTTGATTGAAAAGTGTA+GGG | 0.454603 | 5.1:+57249582 | MS.gene91236:CDS |
GTTGATGTTGGAGGAGGCAC+AGG | 0.457563 | 5.1:+57249631 | MS.gene91236:CDS |
ATTGTCCTTAAGTAGTAATA+CGG | 0.459915 | 5.1:-57249350 | None:intergenic |
AGTGAGAATTTAAACTATGT+TGG | 0.463618 | 5.1:+57249742 | MS.gene91236:CDS |
CTTCTTTGATATGTTGATGA+TGG | 0.474685 | 5.1:+57250078 | MS.gene91236:CDS |
AAGACACTGCACTCCATTTG+TGG | 0.477005 | 5.1:-57249685 | None:intergenic |
TGTGTCGTTTCAAATGTTGT+AGG | 0.487417 | 5.1:-57249466 | None:intergenic |
ACGAATGGCGTCACGCTCAT+TGG | 0.491626 | 5.1:-57249373 | None:intergenic |
TAGGGGAGTATTCAATGGAT+TGG | 0.495288 | 5.1:+57249600 | MS.gene91236:CDS |
GACACACGGGATGTTGATAT+GGG | 0.503724 | 5.1:+57249483 | MS.gene91236:CDS |
AAGTGTAGGGGAGTATTCAA+TGG | 0.504991 | 5.1:+57249595 | MS.gene91236:CDS |
TGAAAATGTGATTCCATATG+TGG | 0.508708 | 5.1:-57249093 | None:intergenic |
AAACTTATTCAATGAGTCCA+TGG | 0.509220 | 5.1:+57249534 | MS.gene91236:CDS |
ATAGCAAAGAAAGGTAAAGA+AGG | 0.509576 | 5.1:+57249983 | MS.gene91236:CDS |
AGACACTGCACTCCATTTGT+GGG | 0.511364 | 5.1:-57249684 | None:intergenic |
CATGTTGTCGATGGCTTGCA+AGG | 0.511966 | 5.1:+57249718 | MS.gene91236:CDS |
GAGAATTTAAACTATGTTGG+TGG | 0.520299 | 5.1:+57249745 | MS.gene91236:CDS |
TTCAACAAAATGGCGTCTGT+TGG | 0.520911 | 5.1:-57249790 | None:intergenic |
AGGCACAGGGACCATGGCAA+AGG | 0.525336 | 5.1:+57249645 | MS.gene91236:CDS |
AAAGAAAGGTAAAGAAGGGA+AGG | 0.534278 | 5.1:+57249988 | MS.gene91236:CDS |
ATTTGTTGATTGAAAAGTGT+AGG | 0.534400 | 5.1:+57249581 | MS.gene91236:CDS |
TCGAGCATCGCATGCACGAA+TGG | 0.541584 | 5.1:-57249388 | None:intergenic |
ATTAAGTTGATTTCTTCGGC+TGG | 0.550414 | 5.1:+57250139 | MS.gene91236:CDS |
GATCTACCACATGTTGTCGA+TGG | 0.552326 | 5.1:+57249709 | MS.gene91236:CDS |
GAGTCATTGGTTGATGTTGG+AGG | 0.554899 | 5.1:+57249622 | MS.gene91236:CDS |
ATGGTGTTGGAAAGTGAGAA+GGG | 0.555376 | 5.1:+57250025 | MS.gene91236:CDS |
TCATTGGTTGATGTTGGAGG+AGG | 0.559528 | 5.1:+57249625 | MS.gene91236:CDS |
CATGGTGTTGGAAAGTGAGA+AGG | 0.565194 | 5.1:+57250024 | MS.gene91236:CDS |
TTGATGATGGTAGTACTCAC+AGG | 0.567324 | 5.1:+57250091 | MS.gene91236:CDS |
TTTAGCAAGGGCCTTTGCCA+TGG | 0.572788 | 5.1:-57249656 | None:intergenic |
AACAACACATTTAAGTGACA+TGG | 0.577803 | 5.1:-57249128 | None:intergenic |
AAGGATGCAATAGCAAAGAA+AGG | 0.578531 | 5.1:+57249974 | MS.gene91236:CDS |
AAGTAGTAATACGGATGCAT+CGG | 0.581276 | 5.1:-57249341 | None:intergenic |
TAGCAAAGAAAGGTAAAGAA+GGG | 0.583690 | 5.1:+57249984 | MS.gene91236:CDS |
TCATTATTCGCATCAAGCGA+TGG | 0.586198 | 5.1:-57249252 | None:intergenic |
TTTGTGGGAATGATTTAGCA+AGG | 0.587728 | 5.1:-57249669 | None:intergenic |
TAATCGAGCATCACTTGCCA+TGG | 0.588822 | 5.1:-57249551 | None:intergenic |
TTGTGGGAATGATTTAGCAA+GGG | 0.591958 | 5.1:-57249668 | None:intergenic |
TGGAGGAGGCACAGGGACCA+TGG | 0.591972 | 5.1:+57249639 | MS.gene91236:CDS |
AAGTAGAGAATTGATGCCAC+GGG | 0.597289 | 5.1:-57249426 | None:intergenic |
GATTGTGATAGACATGGTGT+TGG | 0.599108 | 5.1:+57250012 | MS.gene91236:CDS |
CAAGTAGAGAATTGATGCCA+CGG | 0.608243 | 5.1:-57249427 | None:intergenic |
ACAACATTTGAAACGACACA+CGG | 0.615695 | 5.1:+57249469 | MS.gene91236:CDS |
TTGTTGATTGAAAAGTGTAG+GGG | 0.631295 | 5.1:+57249583 | MS.gene91236:CDS |
TGCAAGCCATCGACAACATG+TGG | 0.637297 | 5.1:-57249715 | None:intergenic |
TTGATGTTGGAGGAGGCACA+GGG | 0.656877 | 5.1:+57249632 | MS.gene91236:CDS |
CAACATTTGAAACGACACAC+GGG | 0.671406 | 5.1:+57249470 | MS.gene91236:CDS |
TTTAAATTTAGAGTCACGCG+CGG | 0.701266 | 5.1:-57249512 | None:intergenic |
GAAGGTGATTGTGATAGACA+TGG | 0.741542 | 5.1:+57250006 | MS.gene91236:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!! | AAAAATATTAAGGAATTGTA+AGG | + | chr5.1:57249955-57249974 | MS.gene91236:CDS | 15.0% |
!! | ATGAATGTGTAAAAATATTA+AGG | + | chr5.1:57249945-57249964 | MS.gene91236:CDS | 15.0% |
!!! | AACATTCTCTTCATTATTTT+GGG | - | chr5.1:57249047-57249066 | None:intergenic | 20.0% |
! | AGTGAGAATTTAAACTATGT+TGG | + | chr5.1:57249742-57249761 | MS.gene91236:CDS | 25.0% |
! | ATTGTCCTTAAGTAGTAATA+CGG | - | chr5.1:57249353-57249372 | None:intergenic | 25.0% |
!! | ACACTTTTCAATCAACAAAT+TGG | - | chr5.1:57249581-57249600 | None:intergenic | 25.0% |
!! | ACATTTTACCTTCAACAAAA+TGG | - | chr5.1:57249803-57249822 | None:intergenic | 25.0% |
!! | ATTTGTTGATTGAAAAGTGT+AGG | + | chr5.1:57249581-57249600 | MS.gene91236:CDS | 25.0% |
!! | CAACATTCTCTTCATTATTT+TGG | - | chr5.1:57249048-57249067 | None:intergenic | 25.0% |
!! | TACAAGATTACTCCAATTTT+AGG | + | chr5.1:57250172-57250191 | MS.gene91236:CDS | 25.0% |
!! | TTTGTTGATTGAAAAGTGTA+GGG | + | chr5.1:57249582-57249601 | MS.gene91236:CDS | 25.0% |
!!! | AATAAGTTTTGAGAGTGATA+TGG | - | chr5.1:57249194-57249213 | None:intergenic | 25.0% |
!!! | ATGGATTAAGTTGATTTCTT+CGG | + | chr5.1:57250135-57250154 | MS.gene91236:CDS | 25.0% |
!!! | CTTAAATGTGTTGTTGATTT+AGG | + | chr5.1:57249136-57249155 | MS.gene91236:CDS | 25.0% |
AAACTTATTCAATGAGTCCA+TGG | + | chr5.1:57249534-57249553 | MS.gene91236:CDS | 30.0% | |
AACAACACATTTAAGTGACA+TGG | - | chr5.1:57249131-57249150 | None:intergenic | 30.0% | |
AAGTCTGAATAAAATTGCAG+TGG | + | chr5.1:57249900-57249919 | MS.gene91236:intron | 30.0% | |
ATAGCAAAGAAAGGTAAAGA+AGG | + | chr5.1:57249983-57250002 | MS.gene91236:CDS | 30.0% | |
CCATAATTGTGTATGATATC+AGG | - | chr5.1:57249166-57249185 | None:intergenic | 30.0% | |
CCTGATATCATACACAATTA+TGG | + | chr5.1:57249163-57249182 | MS.gene91236:CDS | 30.0% | |
GAGAATTTAAACTATGTTGG+TGG | + | chr5.1:57249745-57249764 | MS.gene91236:CDS | 30.0% | |
TAGCAAAGAAAGGTAAAGAA+GGG | + | chr5.1:57249984-57250003 | MS.gene91236:CDS | 30.0% | |
TGAAAATGTGATTCCATATG+TGG | - | chr5.1:57249096-57249115 | None:intergenic | 30.0% | |
! | CTTCTTTGATATGTTGATGA+TGG | + | chr5.1:57250078-57250097 | MS.gene91236:CDS | 30.0% |
! | TTACTCCAATTTTAGGCTTA+AGG | + | chr5.1:57250179-57250198 | MS.gene91236:CDS | 30.0% |
! | TTGTTGATTGAAAAGTGTAG+GGG | + | chr5.1:57249583-57249602 | MS.gene91236:CDS | 30.0% |
AAAGAAAGGTAAAGAAGGGA+AGG | + | chr5.1:57249988-57250007 | MS.gene91236:CDS | 35.0% | |
AAGAGAGAAACGAGAAAGAA+TGG | + | chr5.1:57250116-57250135 | MS.gene91236:CDS | 35.0% | |
AAGGATGCAATAGCAAAGAA+AGG | + | chr5.1:57249974-57249993 | MS.gene91236:CDS | 35.0% | |
AAGTAGTAATACGGATGCAT+CGG | - | chr5.1:57249344-57249363 | None:intergenic | 35.0% | |
AGAGACCTTAAGCCTAAAAT+TGG | - | chr5.1:57250187-57250206 | None:intergenic | 35.0% | |
ATGCGAATAATGACTCATTC+TGG | + | chr5.1:57249262-57249281 | MS.gene91236:CDS | 35.0% | |
TGCATCCGTATTACTACTTA+AGG | + | chr5.1:57249345-57249364 | MS.gene91236:CDS | 35.0% | |
TGCTAAATCATTCCCACAAA+TGG | + | chr5.1:57249672-57249691 | MS.gene91236:CDS | 35.0% | |
TTGTGGGAATGATTTAGCAA+GGG | - | chr5.1:57249671-57249690 | None:intergenic | 35.0% | |
TTTGTGGGAATGATTTAGCA+AGG | - | chr5.1:57249672-57249691 | None:intergenic | 35.0% | |
! | ACAACATTTGAAACGACACA+CGG | + | chr5.1:57249469-57249488 | MS.gene91236:CDS | 35.0% |
! | TTTAAATTTAGAGTCACGCG+CGG | - | chr5.1:57249515-57249534 | None:intergenic | 35.0% |
!! | ATTAAGTTGATTTCTTCGGC+TGG | + | chr5.1:57250139-57250158 | MS.gene91236:CDS | 35.0% |
!! | ATTCAATGGATTGGAGTCAT+TGG | + | chr5.1:57249609-57249628 | MS.gene91236:CDS | 35.0% |
!! | CATGTTTTTGAAGGGTGAAT+TGG | - | chr5.1:57249226-57249245 | None:intergenic | 35.0% |
!! | GCTTTGAGCTTTGAGTAAAT+TGG | - | chr5.1:57249074-57249093 | None:intergenic | 35.0% |
!! | GGATGAAGCATGTTTTTGAA+GGG | - | chr5.1:57249234-57249253 | None:intergenic | 35.0% |
!! | TGGATGAAGCATGTTTTTGA+AGG | - | chr5.1:57249235-57249254 | None:intergenic | 35.0% |
!! | TGTGTCGTTTCAAATGTTGT+AGG | - | chr5.1:57249469-57249488 | None:intergenic | 35.0% |
AAGTAGAGAATTGATGCCAC+GGG | - | chr5.1:57249429-57249448 | None:intergenic | 40.0% | |
CAAGTAGAGAATTGATGCCA+CGG | - | chr5.1:57249430-57249449 | None:intergenic | 40.0% | |
GAAGGTGATTGTGATAGACA+TGG | + | chr5.1:57250006-57250025 | MS.gene91236:CDS | 40.0% | |
GATTGTGATAGACATGGTGT+TGG | + | chr5.1:57250012-57250031 | MS.gene91236:CDS | 40.0% | |
TCAAAGCTCAAAGCCACATA+TGG | + | chr5.1:57249080-57249099 | MS.gene91236:CDS | 40.0% | |
TCATTATTCGCATCAAGCGA+TGG | - | chr5.1:57249255-57249274 | None:intergenic | 40.0% | |
TGCAGTGGATATTGCATGAT+TGG | + | chr5.1:57249915-57249934 | MS.gene91236:intron | 40.0% | |
TTCAACAAAATGGCGTCTGT+TGG | - | chr5.1:57249793-57249812 | None:intergenic | 40.0% | |
! | ATGGTGTTGGAAAGTGAGAA+GGG | + | chr5.1:57250025-57250044 | MS.gene91236:CDS | 40.0% |
! | CAACATTTGAAACGACACAC+GGG | + | chr5.1:57249470-57249489 | MS.gene91236:CDS | 40.0% |
! | TTGGAGTCATTGGTTGATGT+TGG | + | chr5.1:57249619-57249638 | MS.gene91236:CDS | 40.0% |
!! | AACAGACGCCATTTTGTTGA+AGG | + | chr5.1:57249792-57249811 | MS.gene91236:CDS | 40.0% |
!! | AAGTGTAGGGGAGTATTCAA+TGG | + | chr5.1:57249595-57249614 | MS.gene91236:CDS | 40.0% |
!! | TAGGGGAGTATTCAATGGAT+TGG | + | chr5.1:57249600-57249619 | MS.gene91236:CDS | 40.0% |
!! | TTGATGATGGTAGTACTCAC+AGG | + | chr5.1:57250091-57250110 | MS.gene91236:CDS | 40.0% |
AACAAAATGGCGTCTGTTGG+AGG | - | chr5.1:57249790-57249809 | None:intergenic | 45.0% | |
AAGACACTGCACTCCATTTG+TGG | - | chr5.1:57249688-57249707 | None:intergenic | 45.0% | |
AGACACTGCACTCCATTTGT+GGG | - | chr5.1:57249687-57249706 | None:intergenic | 45.0% | |
CGTGGCATCAATTCTCTACT+TGG | + | chr5.1:57249428-57249447 | MS.gene91236:CDS | 45.0% | |
GACACACGGGATGTTGATAT+GGG | + | chr5.1:57249483-57249502 | MS.gene91236:CDS | 45.0% | |
GATCTACCACATGTTGTCGA+TGG | + | chr5.1:57249709-57249728 | MS.gene91236:CDS | 45.0% | |
TAATCGAGCATCACTTGCCA+TGG | - | chr5.1:57249554-57249573 | None:intergenic | 45.0% | |
! | CATGGTGTTGGAAAGTGAGA+AGG | + | chr5.1:57250024-57250043 | MS.gene91236:CDS | 45.0% |
! | GAGTCATTGGTTGATGTTGG+AGG | + | chr5.1:57249622-57249641 | MS.gene91236:CDS | 45.0% |
! | TCATTGGTTGATGTTGGAGG+AGG | + | chr5.1:57249625-57249644 | MS.gene91236:CDS | 45.0% |
!! | ATCCAGTTTTGACAAACCCG+TGG | + | chr5.1:57249410-57249429 | MS.gene91236:CDS | 45.0% |
CGACACACGGGATGTTGATA+TGG | + | chr5.1:57249482-57249501 | MS.gene91236:CDS | 50.0% | |
TGCAAGCCATCGACAACATG+TGG | - | chr5.1:57249718-57249737 | None:intergenic | 50.0% | |
TGCCACGGGTTTGTCAAAAC+TGG | - | chr5.1:57249415-57249434 | None:intergenic | 50.0% | |
TTTAGCAAGGGCCTTTGCCA+TGG | - | chr5.1:57249659-57249678 | None:intergenic | 50.0% | |
! | CATGTTGTCGATGGCTTGCA+AGG | + | chr5.1:57249718-57249737 | MS.gene91236:CDS | 50.0% |
! | TTGATGTTGGAGGAGGCACA+GGG | + | chr5.1:57249632-57249651 | MS.gene91236:CDS | 50.0% |
ACGAATGGCGTCACGCTCAT+TGG | - | chr5.1:57249376-57249395 | None:intergenic | 55.0% | |
! | GTTGATGTTGGAGGAGGCAC+AGG | + | chr5.1:57249631-57249650 | MS.gene91236:CDS | 55.0% |
! | TCGAGCATCGCATGCACGAA+TGG | - | chr5.1:57249391-57249410 | None:intergenic | 55.0% |
AGGCACAGGGACCATGGCAA+AGG | + | chr5.1:57249645-57249664 | MS.gene91236:CDS | 60.0% | |
! | TGGAGGAGGCACAGGGACCA+TGG | + | chr5.1:57249639-57249658 | MS.gene91236:CDS | 65.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
chr5.1 | gene | 57249037 | 57250225 | 57249037 | ID=MS.gene91236 |
chr5.1 | mRNA | 57249037 | 57250225 | 57249037 | ID=MS.gene91236.t1;Parent=MS.gene91236 |
chr5.1 | exon | 57249037 | 57249813 | 57249037 | ID=MS.gene91236.t1.exon1;Parent=MS.gene91236.t1 |
chr5.1 | CDS | 57249037 | 57249813 | 57249037 | ID=cds.MS.gene91236.t1;Parent=MS.gene91236.t1 |
chr5.1 | exon | 57249920 | 57250225 | 57249920 | ID=MS.gene91236.t1.exon2;Parent=MS.gene91236.t1 |
chr5.1 | CDS | 57249920 | 57250225 | 57249920 | ID=cds.MS.gene91236.t1;Parent=MS.gene91236.t1 |
Gene Sequence |
Protein sequence |