AlfalfaGEDB | MS.gene91309

M. sativa cultivar XinJiangDaYe / MS.gene91309

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91309.t1	XP_013469983.1	92.1	76	6	0	1	76	38	113	1.80E-30	141.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91309.t1	A0A072VQS7	92.1	76	6	0	1	76	38	113	1.3e-30	141.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050363	MS.gene91309	0.826457	2.68E-54	-1.69E-46
MS.gene051946	MS.gene91309	0.814711	1.34E-51	-1.69E-46
MS.gene053826	MS.gene91309	0.809592	1.75E-50	-1.69E-46
MS.gene055401	MS.gene91309	0.802646	5.08E-49	-1.69E-46
MS.gene055549	MS.gene91309	0.805672	1.19E-49	-1.69E-46
MS.gene055550	MS.gene91309	0.806941	6.44E-50	-1.69E-46
MS.gene056310	MS.gene91309	0.806582	7.67E-50	-1.69E-46
MS.gene056311	MS.gene91309	0.809611	1.74E-50	-1.69E-46
MS.gene056408	MS.gene91309	0.818108	2.33E-52	-1.69E-46
MS.gene057014	MS.gene91309	0.824282	8.78E-54	-1.69E-46
MS.gene058630	MS.gene91309	0.804016	2.65E-49	-1.69E-46
MS.gene05884	MS.gene91309	0.802293	6.01E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91309.t1	MTR_1g106215	92.105	76	6	0	1	76	38	113	2.95e-47	145

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTAGCTACCACATGTTAT+TGG	0.297025	1.3:-72979342	None:intergenic
ATTTGGTAATGATGGAAGTT+TGG	0.326402	1.3:-72979237	MS.gene91309:CDS
TTGGAATCTGACTCATTAAC+AGG	0.333936	1.3:-72979323	MS.gene91309:CDS
GAGCACAGATTTGGTAATGA+TGG	0.406744	1.3:-72979245	MS.gene91309:CDS
AGCATAAGAAGCAGCAGCAC+TGG	0.414803	1.3:+72979169	None:intergenic
AGCTGTCACAATGATGCCTC+TGG	0.415269	1.3:+72979208	None:intergenic
GAGTTCTTAGAGCACAGATT+TGG	0.435020	1.3:-72979254	MS.gene91309:CDS
TGCTCTAAGAACTCAGTTGT+GGG	0.467329	1.3:+72979263	None:intergenic
TGAAATTCGTTGAGGGTGGT+TGG	0.483463	1.3:+72979144	None:intergenic
GAAATTCGTTGAGGGTGGTT+GGG	0.490978	1.3:+72979145	None:intergenic
GCTGTCACAATGATGCCTCT+GGG	0.532008	1.3:+72979209	None:intergenic
ATGTTGTTGAAATTCGTTGA+GGG	0.532601	1.3:+72979137	None:intergenic
GCAGCACTGGCAGTGGTCGG+AGG	0.539102	1.3:+72979182	None:intergenic
GTGCTCTAAGAACTCAGTTG+TGG	0.553458	1.3:+72979262	None:intergenic
GCAGCAGCACTGGCAGTGGT+CGG	0.562924	1.3:+72979179	None:intergenic
AGAAGCAGCAGCACTGGCAG+TGG	0.577609	1.3:+72979175	None:intergenic
AGACCTGGTTCTTCTATGAG+AGG	0.610651	1.3:+72979298	None:intergenic
TTGTTGAAATTCGTTGAGGG+TGG	0.611122	1.3:+72979140	None:intergenic
GGAAGTTTGGTTAGCCCCAG+AGG	0.621319	1.3:-72979224	MS.gene91309:CDS
CTGTCACAATGATGCCTCTG+GGG	0.650846	1.3:+72979210	None:intergenic
GATCCTCTCATAGAAGAACC+AGG	0.662887	1.3:-72979301	MS.gene91309:CDS
GGGTTGCTGAAAATCAGACC+TGG	0.666499	1.3:+72979283	None:intergenic
AATGTTGTTGAAATTCGTTG+AGG	0.696246	1.3:+72979136	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATGTTGTTGAAATTCGTTG+AGG	+	chr1.3:72979317-72979336	None:intergenic	30.0%
	ATGTTGTTGAAATTCGTTGA+GGG	+	chr1.3:72979316-72979335	None:intergenic	30.0%
!!	ATTTGGTAATGATGGAAGTT+TGG	-	chr1.3:72979213-72979232	MS.gene91309:CDS	30.0%
	TTGGAATCTGACTCATTAAC+AGG	-	chr1.3:72979127-72979146	MS.gene91309:CDS	35.0%
	GAGTTCTTAGAGCACAGATT+TGG	-	chr1.3:72979196-72979215	MS.gene91309:CDS	40.0%
	TTGTTGAAATTCGTTGAGGG+TGG	+	chr1.3:72979313-72979332	None:intergenic	40.0%
!!	GAGCACAGATTTGGTAATGA+TGG	-	chr1.3:72979205-72979224	MS.gene91309:CDS	40.0%
!!	TGCTCTAAGAACTCAGTTGT+GGG	+	chr1.3:72979190-72979209	None:intergenic	40.0%
	GAAATTCGTTGAGGGTGGTT+GGG	+	chr1.3:72979308-72979327	None:intergenic	45.0%
	GATCCTCTCATAGAAGAACC+AGG	-	chr1.3:72979149-72979168	MS.gene91309:CDS	45.0%
	TGAAATTCGTTGAGGGTGGT+TGG	+	chr1.3:72979309-72979328	None:intergenic	45.0%
!	AGACCTGGTTCTTCTATGAG+AGG	+	chr1.3:72979155-72979174	None:intergenic	45.0%
!!	GTGCTCTAAGAACTCAGTTG+TGG	+	chr1.3:72979191-72979210	None:intergenic	45.0%
	AGCATAAGAAGCAGCAGCAC+TGG	+	chr1.3:72979284-72979303	None:intergenic	50.0%
	AGCTGTCACAATGATGCCTC+TGG	+	chr1.3:72979245-72979264	None:intergenic	50.0%
	CTGTCACAATGATGCCTCTG+GGG	+	chr1.3:72979243-72979262	None:intergenic	50.0%
	GCTGTCACAATGATGCCTCT+GGG	+	chr1.3:72979244-72979263	None:intergenic	50.0%
	GGGTTGCTGAAAATCAGACC+TGG	+	chr1.3:72979170-72979189	None:intergenic	50.0%
!	GGAAGTTTGGTTAGCCCCAG+AGG	-	chr1.3:72979226-72979245	MS.gene91309:CDS	55.0%
!!	AGAAGCAGCAGCACTGGCAG+TGG	+	chr1.3:72979278-72979297	None:intergenic	60.0%
!!	GCAGCAGCACTGGCAGTGGT+CGG	+	chr1.3:72979274-72979293	None:intergenic	65.0%
!!	GCAGCACTGGCAGTGGTCGG+AGG	+	chr1.3:72979271-72979290	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	72979121	72979351	72979121	ID=MS.gene91309
chr1.3	mRNA	72979121	72979351	72979121	ID=MS.gene91309.t1;Parent=MS.gene91309
chr1.3	exon	72979121	72979351	72979121	ID=MS.gene91309.t1.exon1;Parent=MS.gene91309.t1
chr1.3	CDS	72979121	72979351	72979121	ID=cds.MS.gene91309.t1;Parent=MS.gene91309.t1

Gene Sequence	>MS.gene91309 ATGTTATTGGAATCTGACTCATTAACAGGATCCTCTCATAGAAGAACCAGGTCTGATTTTCAGCAACCCACAACTGAGTTCTTAGAGCACAGATTTGGTAATGATGGAAGTTTGGTTAGCCCCAGAGGCATCATTGTGACAGCTCTGCCTCCGACCACTGCCAGTGCTGCTGCTTCTTATGCTACCCAACCACCCTCAACGAATTTCAACAACATTCGCAAACACAAATGA
Protein sequence	>MS.gene91309.t1 MLLESDSLTGSSHRRTRSDFQQPTTEFLEHRFGNDGSLVSPRGIIVTALPPTTASAAASYATQPPSTNFNNIRKHK