Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92171.t1 | KEH17167.1 | 90.6 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 1.10E-37 | 165.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92171.t1 | A0A072TID1 | 90.6 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 8.2e-38 | 165.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051544 | MS.gene92171 | 0.81326 | 2.80E-51 | -1.69E-46 |
| MS.gene051932 | MS.gene92171 | 0.850898 | 1.26E-60 | -1.69E-46 |
| MS.gene053356 | MS.gene92171 | 0.806599 | 7.61E-50 | -1.69E-46 |
| MS.gene053491 | MS.gene92171 | 0.846463 | 2.14E-59 | -1.69E-46 |
| MS.gene058426 | MS.gene92171 | 0.809124 | 2.21E-50 | -1.69E-46 |
| MS.gene06044 | MS.gene92171 | 0.823111 | 1.65E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92171.t1 | MTR_0036s0090 | 90.625 | 96 | 9 | 0 | 1 | 96 | 1 | 96 | 7.09e-39 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATAAGTGTTAAACTTCT+TGG | 0.164387 | 2.4:+27558965 | None:intergenic |
| TTTCTTACCAGCCATAGCTT+TGG | 0.395295 | 2.4:-27558792 | MS.gene92171:CDS |
| GAACGAAGCACAAAAGGATA+TGG | 0.440435 | 2.4:-27558997 | MS.gene92171:CDS |
| TGTTCTGTTGACATCAGTTA+AGG | 0.466515 | 2.4:+27558769 | None:intergenic |
| GATGTCAACAGAACAGTTGT+TGG | 0.472083 | 2.4:-27558761 | MS.gene92171:CDS |
| AAGCACAAAAGGATATGGTT+TGG | 0.477101 | 2.4:-27558992 | MS.gene92171:CDS |
| AATCGTGAACGAAGCACAAA+AGG | 0.486280 | 2.4:-27559003 | MS.gene92171:CDS |
| GAGCACTTCCTCAACAAGTT+GGG | 0.488474 | 2.4:-27558846 | MS.gene92171:CDS |
| CACTTATGAAGATGCTCTCA+TGG | 0.494764 | 2.4:-27558952 | MS.gene92171:intron |
| GGAGCACTTCCTCAACAAGT+TGG | 0.533066 | 2.4:-27558847 | MS.gene92171:CDS |
| ATCAGTTAAGGCCAAAGCTA+TGG | 0.533572 | 2.4:+27558781 | None:intergenic |
| AAGTTTCACAGTTGAAGTAG+TGG | 0.566854 | 2.4:-27558738 | MS.gene92171:CDS |
| TTTCACAGTTGAAGTAGTGG+TGG | 0.573034 | 2.4:-27558735 | MS.gene92171:CDS |
| GTTAAGGCCAAAGCTATGGC+TGG | 0.581908 | 2.4:+27558785 | None:intergenic |
| CTTGTTATCCCAACTTGTTG+AGG | 0.598354 | 2.4:+27558838 | None:intergenic |
| AGTAGAAGAATATGATGCCA+TGG | 0.617826 | 2.4:+27559043 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAAGAATCTTTCTAAAAAT+GGG | - | chr2.4:27558824-27558843 | MS.gene92171:CDS | 20.0% |
| !!! | TTCAAGAATCTTTCTAAAAA+TGG | - | chr2.4:27558823-27558842 | MS.gene92171:CDS | 20.0% |
| ! | TTCATAAGTGTTAAACTTCT+TGG | + | chr2.4:27558767-27558786 | None:intergenic | 25.0% |
| GTATGATCTTCTATCTACTA+TGG | - | chr2.4:27558846-27558865 | MS.gene92171:CDS | 30.0% | |
| ! | CTATCTACTATGGTTTTTCA+AGG | - | chr2.4:27558856-27558875 | MS.gene92171:intron | 30.0% |
| ! | TTCACGATTGAAACATTTTC+AGG | + | chr2.4:27558715-27558734 | None:intergenic | 30.0% |
| AAGCACAAAAGGATATGGTT+TGG | - | chr2.4:27558737-27558756 | MS.gene92171:CDS | 35.0% | |
| AAGTTTCACAGTTGAAGTAG+TGG | - | chr2.4:27558991-27559010 | MS.gene92171:CDS | 35.0% | |
| TGTTCTGTTGACATCAGTTA+AGG | + | chr2.4:27558963-27558982 | None:intergenic | 35.0% | |
| !!! | TACTATGGTTTTTCAAGGCT+AGG | - | chr2.4:27558861-27558880 | MS.gene92171:intron | 35.0% |
| AATCGTGAACGAAGCACAAA+AGG | - | chr2.4:27558726-27558745 | MS.gene92171:CDS | 40.0% | |
| ATCAGTTAAGGCCAAAGCTA+TGG | + | chr2.4:27558951-27558970 | None:intergenic | 40.0% | |
| CACTTATGAAGATGCTCTCA+TGG | - | chr2.4:27558777-27558796 | MS.gene92171:CDS | 40.0% | |
| GAACGAAGCACAAAAGGATA+TGG | - | chr2.4:27558732-27558751 | MS.gene92171:CDS | 40.0% | |
| GATGTCAACAGAACAGTTGT+TGG | - | chr2.4:27558968-27558987 | MS.gene92171:CDS | 40.0% | |
| TTTCACAGTTGAAGTAGTGG+TGG | - | chr2.4:27558994-27559013 | MS.gene92171:CDS | 40.0% | |
| TTTCTTACCAGCCATAGCTT+TGG | - | chr2.4:27558937-27558956 | MS.gene92171:intron | 40.0% | |
| !! | CTTGTTATCCCAACTTGTTG+AGG | + | chr2.4:27558894-27558913 | None:intergenic | 40.0% |
| GAGCACTTCCTCAACAAGTT+GGG | - | chr2.4:27558883-27558902 | MS.gene92171:intron | 45.0% | |
| GTTAAGGCCAAAGCTATGGC+TGG | + | chr2.4:27558947-27558966 | None:intergenic | 50.0% | |
| ! | GGAGCACTTCCTCAACAAGT+TGG | - | chr2.4:27558882-27558901 | MS.gene92171:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 27558688 | 27559063 | 27558688 | ID=MS.gene92171 |
| chr2.4 | mRNA | 27558688 | 27559063 | 27558688 | ID=MS.gene92171.t1;Parent=MS.gene92171 |
| chr2.4 | exon | 27558953 | 27559063 | 27558953 | ID=MS.gene92171.t1.exon1;Parent=MS.gene92171.t1 |
| chr2.4 | CDS | 27558953 | 27559063 | 27558953 | ID=cds.MS.gene92171.t1;Parent=MS.gene92171.t1 |
| chr2.4 | exon | 27558688 | 27558873 | 27558688 | ID=MS.gene92171.t1.exon2;Parent=MS.gene92171.t1 |
| chr2.4 | CDS | 27558688 | 27558873 | 27558688 | ID=cds.MS.gene92171.t1;Parent=MS.gene92171.t1 |
| Gene Sequence |
| Protein sequence |
