Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93381.t1 | XP_003604575.1 | 96.5 | 259 | 8 | 1 | 1 | 259 | 17 | 274 | 6.30E-128 | 466.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93381.t1 | G7JLT9 | 96.5 | 259 | 8 | 1 | 1 | 259 | 17 | 274 | 4.6e-128 | 466.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052188 | MS.gene93381 | 0.802338 | 5.88E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene93381 | 0.802263 | 6.10E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene93381 | 0.80476 | 1.85E-49 | -1.69E-46 |
| MS.gene052484 | MS.gene93381 | 0.810581 | 1.07E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene93381 | 0.814146 | 1.79E-51 | -1.69E-46 |
| MS.gene052784 | MS.gene93381 | 0.876075 | 1.86E-68 | -1.69E-46 |
| MS.gene056511 | MS.gene93381 | 0.800689 | 1.28E-48 | -1.69E-46 |
| MS.gene058007 | MS.gene93381 | 0.82917 | 5.98E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene93381 | 0.813535 | 2.44E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene93381 | 0.815713 | 8.03E-52 | -1.69E-46 |
| MS.gene059695 | MS.gene93381 | 0.809955 | 1.46E-50 | -1.69E-46 |
| MS.gene060837 | MS.gene93381 | 0.80324 | 3.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93381.t1 | MTR_4g014670 | 96.911 | 259 | 7 | 1 | 1 | 259 | 17 | 274 | 0.0 | 517 |
| MS.gene93381.t1 | MTR_7g069710 | 46.324 | 272 | 89 | 13 | 1 | 249 | 23 | 260 | 2.56e-51 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCGAAACTTTCTTCCCT+TGG | 0.318412 | 4.1:+81502708 | MS.gene93381:CDS |
| TCTTTGTCATCAAGTTCATT+AGG | 0.362522 | 4.1:+81502155 | MS.gene93381:CDS |
| GATTCTGATAAATAGCATTC+TGG | 0.384127 | 4.1:-81502620 | None:intergenic |
| CCATTCCTACCAGTATCTTT+CGG | 0.386168 | 4.1:-81502236 | None:intergenic |
| GTCGTGGAGCATGGCAGTAC+AGG | 0.404923 | 4.1:-81502767 | None:intergenic |
| TCTTCCGGACGCAACCGCTT+TGG | 0.410420 | 4.1:+81502529 | MS.gene93381:CDS |
| GAATGCTATTTATCAGAATC+AGG | 0.425031 | 4.1:+81502623 | MS.gene93381:CDS |
| GTTTCGAGACCACATCCCTT+TGG | 0.425269 | 4.1:-81502695 | None:intergenic |
| TCGTGGAAACAAACGATGGC+AGG | 0.437134 | 4.1:-81502273 | None:intergenic |
| CCAGGAAAACCGAAAGATAC+TGG | 0.444180 | 4.1:+81502227 | MS.gene93381:CDS |
| GCAGCCAAAGCGGTTGCGTC+CGG | 0.447146 | 4.1:-81502533 | None:intergenic |
| TCTTCTTCTCCAAAGGGATG+TGG | 0.458927 | 4.1:+81502686 | MS.gene93381:CDS |
| GGTTCTTTCTCCCATGAAAA+CGG | 0.468783 | 4.1:-81502206 | None:intergenic |
| CAGCGCAACTCGAGAGAGTC+GGG | 0.479961 | 4.1:+81502812 | MS.gene93381:CDS |
| CTTTCTTCACGTTCGTGCAA+GGG | 0.499824 | 4.1:-81502865 | None:intergenic |
| TGATCATGTAAGTAGGAGAT+TGG | 0.506183 | 4.1:-81502499 | None:intergenic |
| TCTTTCTTCACGTTCGTGCA+AGG | 0.523409 | 4.1:-81502866 | None:intergenic |
| CAAAGATGTGTCTGCAACGA+CGG | 0.528117 | 4.1:-81502138 | None:intergenic |
| CTGCAGATTTGTCGTAGTCG+TGG | 0.532103 | 4.1:-81502783 | None:intergenic |
| CCGAAAGATACTGGTAGGAA+TGG | 0.534229 | 4.1:+81502236 | MS.gene93381:CDS |
| TGATCTTTACTCAGATGCTA+TGG | 0.539239 | 4.1:+81502364 | MS.gene93381:CDS |
| ATTATGTCAACACGTCGTCT+TGG | 0.552256 | 4.1:+81502113 | None:intergenic |
| TTATGCTTCATAGTTCTCCA+AGG | 0.554836 | 4.1:-81502725 | None:intergenic |
| AAACGGTTGATCATGTAAGT+AGG | 0.561511 | 4.1:-81502506 | None:intergenic |
| TTTGTTTCCACGAAAAGAAG+CGG | 0.584227 | 4.1:+81502283 | MS.gene93381:CDS |
| TCGTGGAGCATGGCAGTACA+GGG | 0.592413 | 4.1:-81502766 | None:intergenic |
| CGTGGAAACAAACGATGGCA+GGG | 0.601100 | 4.1:-81502272 | None:intergenic |
| TATGCTTCATAGTTCTCCAA+GGG | 0.609801 | 4.1:-81502724 | None:intergenic |
| TTAAAGCTTGCAGAGTGTAG+TGG | 0.611037 | 4.1:+81502473 | MS.gene93381:CDS |
| TCAGCGCAACTCGAGAGAGT+CGG | 0.612829 | 4.1:+81502811 | MS.gene93381:CDS |
| GAAAACCGAAAGATACTGGT+AGG | 0.618943 | 4.1:+81502231 | MS.gene93381:CDS |
| TTTGTCGTAGTCGTGGAGCA+TGG | 0.626564 | 4.1:-81502776 | None:intergenic |
| CGGTTGCGTCCGGAAGAAAA+CGG | 0.640456 | 4.1:-81502523 | None:intergenic |
| AGACGATGATGCAGCCAAAG+CGG | 0.673928 | 4.1:-81502543 | None:intergenic |
| AGTCGGGACAGAAGAAACAA+CGG | 0.729959 | 4.1:+81502828 | MS.gene93381:CDS |
| GGAAACAAACGATGGCAGGG+TGG | 0.758125 | 4.1:-81502269 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATGTAAAAAATGATTGTGA+TGG | + | chr4.1:81502317-81502336 | MS.gene93381:CDS | 25.0% |
| GATTCTGATAAATAGCATTC+TGG | - | chr4.1:81502623-81502642 | None:intergenic | 30.0% | |
| TCTTTGTCATCAAGTTCATT+AGG | + | chr4.1:81502155-81502174 | MS.gene93381:CDS | 30.0% | |
| ! | AAAAAATGATTGTGATGGAG+AGG | + | chr4.1:81502322-81502341 | MS.gene93381:CDS | 30.0% |
| ! | GAATGCTATTTATCAGAATC+AGG | + | chr4.1:81502623-81502642 | MS.gene93381:CDS | 30.0% |
| AAACGGTTGATCATGTAAGT+AGG | - | chr4.1:81502509-81502528 | None:intergenic | 35.0% | |
| TATGCTTCATAGTTCTCCAA+GGG | - | chr4.1:81502727-81502746 | None:intergenic | 35.0% | |
| TCTTCTTCTTCTTCTCCAAA+GGG | + | chr4.1:81502680-81502699 | MS.gene93381:CDS | 35.0% | |
| TGATCATGTAAGTAGGAGAT+TGG | - | chr4.1:81502502-81502521 | None:intergenic | 35.0% | |
| TGATCTTTACTCAGATGCTA+TGG | + | chr4.1:81502364-81502383 | MS.gene93381:CDS | 35.0% | |
| TTATGCTTCATAGTTCTCCA+AGG | - | chr4.1:81502728-81502747 | None:intergenic | 35.0% | |
| TTCTTCTTCTTCTTCTCCAA+AGG | + | chr4.1:81502679-81502698 | MS.gene93381:CDS | 35.0% | |
| TTTGTTTCCACGAAAAGAAG+CGG | + | chr4.1:81502283-81502302 | MS.gene93381:CDS | 35.0% | |
| ! | TGAAAGAGTTCCGTTTTCAT+GGG | + | chr4.1:81502196-81502215 | MS.gene93381:CDS | 35.0% |
| CCATTCCTACCAGTATCTTT+CGG | - | chr4.1:81502239-81502258 | None:intergenic | 40.0% | |
| GAAAACCGAAAGATACTGGT+AGG | + | chr4.1:81502231-81502250 | MS.gene93381:CDS | 40.0% | |
| GGTTCTTTCTCCCATGAAAA+CGG | - | chr4.1:81502209-81502228 | None:intergenic | 40.0% | |
| TTAAAGCTTGCAGAGTGTAG+TGG | + | chr4.1:81502473-81502492 | MS.gene93381:CDS | 40.0% | |
| ! | CATGATCAACCGTTTTCTTC+CGG | + | chr4.1:81502514-81502533 | MS.gene93381:CDS | 40.0% |
| ! | CTTTTCGTGGAAACAAACGA+TGG | - | chr4.1:81502280-81502299 | None:intergenic | 40.0% |
| ! | GTGAAAGAGTTCCGTTTTCA+TGG | + | chr4.1:81502195-81502214 | MS.gene93381:CDS | 40.0% |
| ! | TTTTCATGGGAGAAAGAACC+AGG | + | chr4.1:81502209-81502228 | MS.gene93381:CDS | 40.0% |
| AGTCGGGACAGAAGAAACAA+CGG | + | chr4.1:81502828-81502847 | MS.gene93381:CDS | 45.0% | |
| CAAAGATGTGTCTGCAACGA+CGG | - | chr4.1:81502141-81502160 | None:intergenic | 45.0% | |
| CCAGGAAAACCGAAAGATAC+TGG | + | chr4.1:81502227-81502246 | MS.gene93381:CDS | 45.0% | |
| CCGAAAGATACTGGTAGGAA+TGG | + | chr4.1:81502236-81502255 | MS.gene93381:CDS | 45.0% | |
| CTTTCTTCACGTTCGTGCAA+GGG | - | chr4.1:81502868-81502887 | None:intergenic | 45.0% | |
| GTCTCGAAACTTTCTTCCCT+TGG | + | chr4.1:81502708-81502727 | MS.gene93381:CDS | 45.0% | |
| TCTTCTTCTCCAAAGGGATG+TGG | + | chr4.1:81502686-81502705 | MS.gene93381:CDS | 45.0% | |
| TCTTTCTTCACGTTCGTGCA+AGG | - | chr4.1:81502869-81502888 | None:intergenic | 45.0% | |
| ! | CCAGTATCTTTCGGTTTTCC+TGG | - | chr4.1:81502230-81502249 | None:intergenic | 45.0% |
| AGACGATGATGCAGCCAAAG+CGG | - | chr4.1:81502546-81502565 | None:intergenic | 50.0% | |
| CGTGGAAACAAACGATGGCA+GGG | - | chr4.1:81502275-81502294 | None:intergenic | 50.0% | |
| CTGCAGATTTGTCGTAGTCG+TGG | - | chr4.1:81502786-81502805 | None:intergenic | 50.0% | |
| GTTTCGAGACCACATCCCTT+TGG | - | chr4.1:81502698-81502717 | None:intergenic | 50.0% | |
| TCGTGGAAACAAACGATGGC+AGG | - | chr4.1:81502276-81502295 | None:intergenic | 50.0% | |
| TTTGTCGTAGTCGTGGAGCA+TGG | - | chr4.1:81502779-81502798 | None:intergenic | 50.0% | |
| ! | TGATCAGCCGCTTCTTTTCG+TGG | - | chr4.1:81502293-81502312 | None:intergenic | 50.0% |
| CGGTTGCGTCCGGAAGAAAA+CGG | - | chr4.1:81502526-81502545 | None:intergenic | 55.0% | |
| GGAAACAAACGATGGCAGGG+TGG | - | chr4.1:81502272-81502291 | None:intergenic | 55.0% | |
| TCAGCGCAACTCGAGAGAGT+CGG | + | chr4.1:81502811-81502830 | MS.gene93381:CDS | 55.0% | |
| TCGTGGAGCATGGCAGTACA+GGG | - | chr4.1:81502769-81502788 | None:intergenic | 55.0% | |
| CAGCGCAACTCGAGAGAGTC+GGG | + | chr4.1:81502812-81502831 | MS.gene93381:CDS | 60.0% | |
| GTCGTGGAGCATGGCAGTAC+AGG | - | chr4.1:81502770-81502789 | None:intergenic | 60.0% | |
| TCTTCCGGACGCAACCGCTT+TGG | + | chr4.1:81502529-81502548 | MS.gene93381:CDS | 60.0% | |
| GCAGCCAAAGCGGTTGCGTC+CGG | - | chr4.1:81502536-81502555 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 81502116 | 81502895 | 81502116 | ID=MS.gene93381 |
| chr4.1 | mRNA | 81502116 | 81502895 | 81502116 | ID=MS.gene93381.t1;Parent=MS.gene93381 |
| chr4.1 | exon | 81502116 | 81502895 | 81502116 | ID=MS.gene93381.t1.exon1;Parent=MS.gene93381.t1 |
| chr4.1 | CDS | 81502116 | 81502895 | 81502116 | ID=cds.MS.gene93381.t1;Parent=MS.gene93381.t1 |
| Gene Sequence |
| Protein sequence |
