AlfalfaGEDB | MS.gene95575

M. sativa cultivar XinJiangDaYe / MS.gene95575

KEGG: K09875
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95575.t1	XP_003605942.1	98.3	239	4	0	1	239	1	239	1.50E-123	452.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95575.t1	Q9ATM2	48.3	236	120	2	4	239	7	240	7.1e-58	225.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95575.t1	G7JDK7	98.3	239	4	0	1	239	1	239	1.1e-123	452.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049351	MS.gene95575	0.809512	1.82E-50	-1.69E-46
MS.gene049758	MS.gene95575	0.817298	3.54E-52	-1.69E-46
MS.gene050397	MS.gene95575	0.82307	1.69E-53	-1.69E-46
MS.gene053836	MS.gene95575	0.827349	1.64E-54	-1.69E-46
MS.gene055277	MS.gene95575	0.81514	1.08E-51	-1.69E-46
MS.gene055428	MS.gene95575	0.808259	3.38E-50	-1.69E-46
MS.gene058002	MS.gene95575	0.811935	5.46E-51	-1.69E-46
MS.gene05811	MS.gene95575	0.81587	7.41E-52	-1.69E-46
MS.gene058710	MS.gene95575	0.806135	9.53E-50	-1.69E-46
MS.gene05907	MS.gene95575	0.819972	8.77E-53	-1.69E-46
MS.gene06037	MS.gene95575	0.817768	2.78E-52	-1.69E-46
MS.gene060403	MS.gene95575	0.815124	1.09E-51	-1.69E-46
MS.gene061049	MS.gene95575	0.805841	1.10E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95575.t1	MTR_4g049340	98.326	239	4	0	1	239	1	239	3.29e-170	468
MS.gene95575.t1	MTR_7g085070	52.653	245	109	3	1	239	3	246	3.65e-79	238
MS.gene95575.t1	MTR_6g033010	51.915	235	107	2	4	233	5	238	1.58e-73	224
MS.gene95575.t1	MTR_6g033010	52.041	196	88	2	4	194	5	199	2.80e-53	171
MS.gene95575.t1	MTR_6g033010	52.356	191	85	2	4	189	5	194	2.22e-52	169
MS.gene95575.t1	MTR_7g088310	34.375	192	116	5	49	239	50	232	1.32e-25	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95575.t1	AT3G04090	46.957	230	118	2	1	230	2	227	1.31e-60	191
MS.gene95575.t1	AT5G18290	42.623	244	134	3	1	239	1	243	2.83e-54	175
MS.gene95575.t1	AT5G18290	42.623	244	134	3	1	239	1	243	2.83e-54	175
MS.gene95575.t1	AT3G56950	29.461	241	156	5	1	238	1	230	2.59e-24	97.4
MS.gene95575.t1	AT3G56950	29.461	241	156	5	1	238	1	230	4.39e-24	97.1

Find 57 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCCTCACAATAAGCCTTAT+TGG	0.201441	4.3:+50195107	MS.gene95575:CDS
TATTCTTCTTGTTATGCTTA+AGG	0.242398	4.3:+50195388	MS.gene95575:CDS
TTCTGTGGCAACTTTGGTTT+TGG	0.253700	4.3:+50195445	MS.gene95575:CDS
ATTCTTCTTGTTATGCTTAA+GGG	0.255110	4.3:+50195389	MS.gene95575:CDS
CACCTAAGATTCTTCCAATA+AGG	0.292588	4.3:-50195121	None:intergenic
ATTATAGGTTCTGGTTATAC+TGG	0.294451	4.3:+50195470	MS.gene95575:CDS
CCCTGCTAATGCATTTGGTT+GGG	0.316346	4.3:+50195505	MS.gene95575:CDS
TCTATTTATGTCACCGGTTA+AGG	0.319094	4.3:+50195625	MS.gene95575:CDS
ACAATTTCCTTCTACGCATT+AGG	0.342644	4.3:+50195167	MS.gene95575:CDS
ATGAACCCTGCTAATGCATT+TGG	0.350303	4.3:+50195500	MS.gene95575:CDS
AGCCTTATTGGAAGAATCTT+AGG	0.350741	4.3:+50195119	MS.gene95575:CDS
CAAGCTTTCTTCTCCTTAAC+CGG	0.351841	4.3:-50195638	None:intergenic
TTGAAAGTTGATTTGCATAC+AGG	0.359134	4.3:+50195323	MS.gene95575:CDS
TTTGGATATGTCCTTTACTT+GGG	0.390759	4.3:+50195573	MS.gene95575:CDS
TTTGCTTTCTGTGGCAACTT+TGG	0.391117	4.3:+50195439	MS.gene95575:CDS
TGCTTGAGCAGGAAACCTTA+TGG	0.401263	4.3:-50195222	None:intergenic
TCTTAGTCCTAATGCGTAGA+AGG	0.406571	4.3:-50195174	None:intergenic
TTAAGATGGTTGTGATGAAT+AGG	0.418484	4.3:-50195073	None:intergenic
TGGGGCTAGTTCTGCTGCTA+TGG	0.418523	4.3:+50195592	MS.gene95575:CDS
ACCATGTCTTTGTATTCTGT+TGG	0.425048	4.3:-50195287	None:intergenic
AGGTTTCCTGCTCAAGCAAT+AGG	0.431036	4.3:+50195227	MS.gene95575:CDS
CAAGCAATAGGTGGAGCAAT+TGG	0.444422	4.3:+50195239	MS.gene95575:CDS
GCCCAACCAAATGCATTAGC+AGG	0.448752	4.3:-50195506	None:intergenic
AATAGGCCTGGTAGTGAAAC+TGG	0.449429	4.3:-50195056	None:intergenic
GCTCCACCTATTGCTTGAGC+AGG	0.471313	4.3:-50195233	None:intergenic
ATTCTGTTGGTATAACTTGA+AGG	0.478894	4.3:-50195274	None:intergenic
GCATTAGCAGGGTTCATAGA+AGG	0.483858	4.3:-50195494	None:intergenic
TAGAAGGAAATTGTGGATGA+AGG	0.490516	4.3:-50195158	None:intergenic
TGTTAGAATTGCATCTCCTA+TGG	0.491217	4.3:-50194961	None:intergenic
GCAGAACTAGCCCCAAGTAA+AGG	0.494786	4.3:-50195584	None:intergenic
TTGGATATGTCCTTTACTTG+GGG	0.500215	4.3:+50195574	MS.gene95575:CDS
TGCAAATCAACTTTCAAAGA+AGG	0.500886	4.3:-50195317	None:intergenic
TGAACAATAAGCATAATACT+TGG	0.504932	4.3:+50195534	MS.gene95575:CDS
ACCCTGCTAATGCATTTGGT+TGG	0.505171	4.3:+50195504	MS.gene95575:CDS
TGTTGGTATAACTTGAAGGA+TGG	0.506984	4.3:-50195270	None:intergenic
AAAGATGGAAGAGATGAATC+TGG	0.509279	4.3:-50195197	None:intergenic
GATAATGATTGTGTTTAAGA+TGG	0.513674	4.3:-50195087	None:intergenic
CATACAGGTGCTATTGCTGA+AGG	0.523194	4.3:+50195338	MS.gene95575:CDS
GGAAACCTTATGGCAAAAGA+TGG	0.524658	4.3:-50195212	None:intergenic
GTGAAACTGGTTGAAGGTTG+AGG	0.525730	4.3:-50195043	None:intergenic
GAAGGTTTATTTGCTTTCTG+TGG	0.527749	4.3:+50195430	MS.gene95575:CDS
CTTCAACCAGTTTCACTACC+AGG	0.541063	4.3:+50195050	MS.gene95575:CDS
TACAGGTGCTATTGCTGAAG+GGG	0.547592	4.3:+50195340	MS.gene95575:CDS
AGCAAATAAACCTTCAACCA+AGG	0.552634	4.3:-50195422	None:intergenic
ATACAGGTGCTATTGCTGAA+GGG	0.571193	4.3:+50195339	MS.gene95575:CDS
TTCCAATAAGGCTTATTGTG+AGG	0.575912	4.3:-50195109	None:intergenic
AGAAGGAAATTGTGGATGAA+GGG	0.578740	4.3:-50195157	None:intergenic
CTGGTAGTGAAACTGGTTGA+AGG	0.582981	4.3:-50195049	None:intergenic
TAATGCGTAGAAGGAAATTG+TGG	0.589400	4.3:-50195165	None:intergenic
GAACAATAAGCATAATACTT+GGG	0.590254	4.3:+50195535	MS.gene95575:CDS
CCCAACCAAATGCATTAGCA+GGG	0.593803	4.3:-50195505	None:intergenic
GAATACAAAGACATGGTGAA+AGG	0.602834	4.3:+50195293	MS.gene95575:CDS
GGAGTGATTAAATCAGCCAT+AGG	0.613343	4.3:+50194945	MS.gene95575:CDS
ATGGTTGTGATGAATAGGCC+TGG	0.622121	4.3:-50195068	None:intergenic
TTTCCTGCTCAAGCAATAGG+TGG	0.642045	4.3:+50195230	MS.gene95575:CDS
CTTATTGGAAGAATCTTAGG+TGG	0.659314	4.3:+50195122	MS.gene95575:CDS
ACCAACAGAATACAAAGACA+TGG	0.676539	4.3:+50195286	MS.gene95575:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAACAATAAGCATAATACTT+GGG	+	chr4.3:50195535-50195554	MS.gene95575:CDS	25.0%
!	GATAATGATTGTGTTTAAGA+TGG	-	chr4.3:50195090-50195109	None:intergenic	25.0%
!	TGAACAATAAGCATAATACT+TGG	+	chr4.3:50195534-50195553	MS.gene95575:CDS	25.0%
!!	ATTCTTCTTGTTATGCTTAA+GGG	+	chr4.3:50195389-50195408	MS.gene95575:CDS	25.0%
!!	TATTCTTCTTGTTATGCTTA+AGG	+	chr4.3:50195388-50195407	MS.gene95575:CDS	25.0%
!!!	ACTTTGGTTTTGGTTATTAT+AGG	+	chr4.3:50195455-50195474	MS.gene95575:CDS	25.0%
	ATTCTGTTGGTATAACTTGA+AGG	-	chr4.3:50195277-50195296	None:intergenic	30.0%
	TGCAAATCAACTTTCAAAGA+AGG	-	chr4.3:50195320-50195339	None:intergenic	30.0%
	TTAAGATGGTTGTGATGAAT+AGG	-	chr4.3:50195076-50195095	None:intergenic	30.0%
	TTTGGATATGTCCTTTACTT+GGG	+	chr4.3:50195573-50195592	MS.gene95575:CDS	30.0%
!	ATTATAGGTTCTGGTTATAC+TGG	+	chr4.3:50195470-50195489	MS.gene95575:CDS	30.0%
!	GGGAGCAATTTTATGTTTTT+TGG	+	chr4.3:50195555-50195574	MS.gene95575:CDS	30.0%
!	TCGTTTTCTATTTATGTCAC+CGG	+	chr4.3:50195619-50195638	MS.gene95575:CDS	30.0%
!	TTTTGGATATGTCCTTTACT+TGG	+	chr4.3:50195572-50195591	MS.gene95575:CDS	30.0%
!!	TTGAAAGTTGATTTGCATAC+AGG	+	chr4.3:50195323-50195342	MS.gene95575:CDS	30.0%
!!!	GTTTTGGTTATTATAGGTTC+TGG	+	chr4.3:50195461-50195480	MS.gene95575:CDS	30.0%
	AAAGATGGAAGAGATGAATC+TGG	-	chr4.3:50195200-50195219	None:intergenic	35.0%
	ACAATTTCCTTCTACGCATT+AGG	+	chr4.3:50195167-50195186	MS.gene95575:CDS	35.0%
	ACCAACAGAATACAAAGACA+TGG	+	chr4.3:50195286-50195305	MS.gene95575:CDS	35.0%
	ACCATGTCTTTGTATTCTGT+TGG	-	chr4.3:50195290-50195309	None:intergenic	35.0%
	AGAAGGAAATTGTGGATGAA+GGG	-	chr4.3:50195160-50195179	None:intergenic	35.0%
	AGCAAATAAACCTTCAACCA+AGG	-	chr4.3:50195425-50195444	None:intergenic	35.0%
	CACCTAAGATTCTTCCAATA+AGG	-	chr4.3:50195124-50195143	None:intergenic	35.0%
	CTTATTGGAAGAATCTTAGG+TGG	+	chr4.3:50195122-50195141	MS.gene95575:CDS	35.0%
	GAAGGTTTATTTGCTTTCTG+TGG	+	chr4.3:50195430-50195449	MS.gene95575:CDS	35.0%
	GAATACAAAGACATGGTGAA+AGG	+	chr4.3:50195293-50195312	MS.gene95575:CDS	35.0%
	TAATGCGTAGAAGGAAATTG+TGG	-	chr4.3:50195168-50195187	None:intergenic	35.0%
	TAGAAGGAAATTGTGGATGA+AGG	-	chr4.3:50195161-50195180	None:intergenic	35.0%
	TCCTAAAAATCCTTGGTTGA+AGG	+	chr4.3:50195412-50195431	MS.gene95575:CDS	35.0%
	TGTTAGAATTGCATCTCCTA+TGG	-	chr4.3:50194964-50194983	None:intergenic	35.0%
	TGTTGGTATAACTTGAAGGA+TGG	-	chr4.3:50195273-50195292	None:intergenic	35.0%
	TTAAGGGTCCTAAAAATCCT+TGG	+	chr4.3:50195405-50195424	MS.gene95575:CDS	35.0%
	TTCCAATAAGGCTTATTGTG+AGG	-	chr4.3:50195112-50195131	None:intergenic	35.0%
	TTGGATATGTCCTTTACTTG+GGG	+	chr4.3:50195574-50195593	MS.gene95575:CDS	35.0%
!	ACCTTCAACCAAGGATTTTT+AGG	-	chr4.3:50195416-50195435	None:intergenic	35.0%
!	ATCCTCACAATAAGCCTTAT+TGG	+	chr4.3:50195107-50195126	MS.gene95575:CDS	35.0%
!	TCTATTTATGTCACCGGTTA+AGG	+	chr4.3:50195625-50195644	MS.gene95575:CDS	35.0%
!!	AGCCTTATTGGAAGAATCTT+AGG	+	chr4.3:50195119-50195138	MS.gene95575:CDS	35.0%
	ATGAACCCTGCTAATGCATT+TGG	+	chr4.3:50195500-50195519	MS.gene95575:CDS	40.0%
	CAAGCTTTCTTCTCCTTAAC+CGG	-	chr4.3:50195641-50195660	None:intergenic	40.0%
	GGAAACCTTATGGCAAAAGA+TGG	-	chr4.3:50195215-50195234	None:intergenic	40.0%
	GGAGTGATTAAATCAGCCAT+AGG	+	chr4.3:50194945-50194964	MS.gene95575:CDS	40.0%
	TCTTAGTCCTAATGCGTAGA+AGG	-	chr4.3:50195177-50195196	None:intergenic	40.0%
	TTTGCTTTCTGTGGCAACTT+TGG	+	chr4.3:50195439-50195458	MS.gene95575:CDS	40.0%
!	CTCTTCCATCTTTTGCCATA+AGG	+	chr4.3:50195207-50195226	MS.gene95575:CDS	40.0%
!!	ATACAGGTGCTATTGCTGAA+GGG	+	chr4.3:50195339-50195358	MS.gene95575:CDS	40.0%
!!	TTCTGTGGCAACTTTGGTTT+TGG	+	chr4.3:50195445-50195464	MS.gene95575:CDS	40.0%
	ACCCTGCTAATGCATTTGGT+TGG	+	chr4.3:50195504-50195523	MS.gene95575:CDS	45.0%
	AGGTTTCCTGCTCAAGCAAT+AGG	+	chr4.3:50195227-50195246	MS.gene95575:CDS	45.0%
	ATGGTTGTGATGAATAGGCC+TGG	-	chr4.3:50195071-50195090	None:intergenic	45.0%
	CAAGCAATAGGTGGAGCAAT+TGG	+	chr4.3:50195239-50195258	MS.gene95575:CDS	45.0%
	CCCAACCAAATGCATTAGCA+GGG	-	chr4.3:50195508-50195527	None:intergenic	45.0%
	CCCTGCTAATGCATTTGGTT+GGG	+	chr4.3:50195505-50195524	MS.gene95575:CDS	45.0%
	CTTCAACCAGTTTCACTACC+AGG	+	chr4.3:50195050-50195069	MS.gene95575:CDS	45.0%
	GCATTAGCAGGGTTCATAGA+AGG	-	chr4.3:50195497-50195516	None:intergenic	45.0%
	GTGAAACTGGTTGAAGGTTG+AGG	-	chr4.3:50195046-50195065	None:intergenic	45.0%
	TGCTTGAGCAGGAAACCTTA+TGG	-	chr4.3:50195225-50195244	None:intergenic	45.0%
	TTTCCTGCTCAAGCAATAGG+TGG	+	chr4.3:50195230-50195249	MS.gene95575:CDS	45.0%
!	AATAGGCCTGGTAGTGAAAC+TGG	-	chr4.3:50195059-50195078	None:intergenic	45.0%
!	CTGGTAGTGAAACTGGTTGA+AGG	-	chr4.3:50195052-50195071	None:intergenic	45.0%
!!	CATACAGGTGCTATTGCTGA+AGG	+	chr4.3:50195338-50195357	MS.gene95575:CDS	45.0%
!!	TACAGGTGCTATTGCTGAAG+GGG	+	chr4.3:50195340-50195359	MS.gene95575:CDS	45.0%
	GCAGAACTAGCCCCAAGTAA+AGG	-	chr4.3:50195587-50195606	None:intergenic	50.0%
	GCCCAACCAAATGCATTAGC+AGG	-	chr4.3:50195509-50195528	None:intergenic	50.0%
	GCTCCACCTATTGCTTGAGC+AGG	-	chr4.3:50195236-50195255	None:intergenic	55.0%
!!	TGGGGCTAGTTCTGCTGCTA+TGG	+	chr4.3:50195592-50195611	MS.gene95575:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	50194942	50195661	50194942	ID=MS.gene95575
chr4.3	mRNA	50194942	50195661	50194942	ID=MS.gene95575.t1;Parent=MS.gene95575
chr4.3	exon	50194942	50195661	50194942	ID=MS.gene95575.t1.exon1;Parent=MS.gene95575.t1
chr4.3	CDS	50194942	50195661	50194942	ID=cds.MS.gene95575.t1;Parent=MS.gene95575.t1

Gene Sequence

>MS.gene95575

ATGGGAGTGATTAAATCAGCCATAGGAGATGCAATTCTAACATCAATATTTGTTTTCTTCATCTCAACATTAAGAATTCTCTCTACAAAAACATCTCTTTTCCTCAACCTTCAACCAGTTTCACTACCAGGCCTATTCATCACAACCATCTTAAACACAATCATTATCCTCACAATAAGCCTTATTGGAAGAATCTTAGGTGGTGCTAGCTTCAACCCTTCATCCACAATTTCCTTCTACGCATTAGGACTAAGACCAGATTCATCTCTTCCATCTTTTGCCATAAGGTTTCCTGCTCAAGCAATAGGTGGAGCAATTGGCATCAAATCCATCCTTCAAGTTATACCAACAGAATACAAAGACATGGTGAAAGGTCCTTCTTTGAAAGTTGATTTGCATACAGGTGCTATTGCTGAAGGGGTTTTGACTTTTACTCATAACTTTGTTATTCTTCTTGTTATGCTTAAGGGTCCTAAAAATCCTTGGTTGAAGGTTTATTTGCTTTCTGTGGCAACTTTGGTTTTGGTTATTATAGGTTCTGGTTATACTGGACCTTCTATGAACCCTGCTAATGCATTTGGTTGGGCTTATATGAACAATAAGCATAATACTTGGGAGCAATTTTATGTTTTTTGGATATGTCCTTTACTTGGGGCTAGTTCTGCTGCTATGGTTTATCGTTTTCTATTTATGTCACCGGTTAAGGAGAAGAAAGCTTGA

Protein sequence

>MS.gene95575.t1

MGVIKSAIGDAILTSIFVFFISTLRILSTKTSLFLNLQPVSLPGLFITTILNTIIILTISLIGRILGGASFNPSSTISFYALGLRPDSSLPSFAIRFPAQAIGGAIGIKSILQVIPTEYKDMVKGPSLKVDLHTGAIAEGVLTFTHNFVILLVMLKGPKNPWLKVYLLSVATLVLVIIGSGYTGPSMNPANAFGWAYMNNKHNTWEQFYVFWICPLLGASSAAMVYRFLFMSPVKEKKA