AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99096


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99096.t1 MTR_5g076170 94.677 263 9 1 1 258 1 263 0.0 498
MS.gene99096.t1 MTR_3g063310 62.791 258 89 3 1 258 2 252 5.47e-100 292
MS.gene99096.t1 MTR_1g077560 48.462 260 122 7 1 258 1 250 1.22e-68 212
MS.gene99096.t1 MTR_5g087760 38.424 203 118 3 59 258 56 254 9.31e-43 146
MS.gene99096.t1 MTR_3g078800 37.566 189 109 4 74 258 69 252 1.69e-34 125
MS.gene99096.t1 MTR_2g012550 35.751 193 119 3 69 258 74 264 3.74e-32 119
MS.gene99096.t1 MTR_2g011350 32.099 162 103 3 86 244 35 192 2.13e-21 89.4
MS.gene99096.t1 MTR_3g078820 32.450 151 95 3 93 240 44 190 3.05e-20 86.3
MS.gene99096.t1 MTR_3g074150 26.840 231 146 8 19 243 19 232 4.70e-19 84.0
MS.gene99096.t1 MTR_7g106000 31.771 192 117 6 54 236 20 206 5.88e-19 83.2
MS.gene99096.t1 MTR_2g100060 33.939 165 105 3 70 232 60 222 3.24e-18 82.0
MS.gene99096.t1 MTR_1g057400 29.947 187 122 5 57 236 3 187 3.76e-18 80.5
MS.gene99096.t1 MTR_3g078810 37.008 127 77 3 121 245 9 134 8.68e-18 78.2
MS.gene99096.t1 MTR_2g090225 30.435 161 109 3 99 258 62 220 9.87e-18 79.7
MS.gene99096.t1 MTR_3g104460 27.363 201 133 5 67 258 57 253 1.77e-17 79.7
MS.gene99096.t1 MTR_7g105970 27.461 193 133 4 65 252 32 222 5.81e-17 77.8
MS.gene99096.t1 MTR_6g071410 31.847 157 95 4 68 217 2 153 1.35e-16 76.6
MS.gene99096.t1 MTR_6g070890 31.847 157 95 4 68 217 2 153 1.35e-16 76.6
MS.gene99096.t1 MTR_6g070900 34.395 157 91 7 68 217 2 153 2.15e-16 75.9
MS.gene99096.t1 MTR_6g071400 34.395 157 91 7 68 217 2 153 2.15e-16 75.9
MS.gene99096.t1 MTR_1g057270 27.746 173 115 5 92 257 74 243 7.22e-16 75.1
MS.gene99096.t1 MTR_4g119428 32.979 188 114 7 65 244 41 224 1.34e-15 74.3
MS.gene99096.t1 MTR_2g086790 25.641 195 132 2 75 256 19 213 1.47e-15 73.9
MS.gene99096.t1 MTR_7g106050 29.379 177 124 1 76 252 22 197 4.46e-15 72.4
MS.gene99096.t1 MTR_7g106120 33.871 124 75 3 61 178 4 126 9.74e-15 71.2
MS.gene99096.t1 MTR_7g106010 30.233 215 128 9 57 256 20 227 1.75e-14 70.9
MS.gene99096.t1 MTR_6g471090 31.788 151 92 5 68 217 11 151 4.83e-14 69.3
MS.gene99096.t1 MTR_7g106030 29.064 203 133 7 50 244 16 215 6.87e-14 69.3
MS.gene99096.t1 MTR_8g016370 28.571 196 110 5 42 232 25 195 1.06e-13 68.9
MS.gene99096.t1 MTR_4g051880 30.769 169 106 4 98 256 44 211 1.28e-13 68.6
MS.gene99096.t1 MTR_2g087000 25.568 176 127 4 70 244 45 217 2.46e-11 62.4
MS.gene99096.t1 MTR_8g090320 25.888 197 123 6 64 255 79 257 3.23e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99096.t1 AT1G65690 52.075 265 107 3 1 258 1 252 1.12e-90 268
MS.gene99096.t1 AT5G36970 52.107 261 109 5 1 258 1 248 1.64e-87 260
MS.gene99096.t1 AT1G54540 44.961 258 123 3 1 258 1 239 4.68e-63 197
MS.gene99096.t1 AT2G27080 37.500 192 116 3 62 252 65 253 1.48e-39 138
MS.gene99096.t1 AT2G27080 37.500 192 116 3 62 252 65 253 1.48e-39 138
MS.gene99096.t1 AT5G21130 28.649 185 132 0 62 246 86 270 1.74e-26 104
MS.gene99096.t1 AT3G11650 32.967 182 118 3 65 244 45 224 6.49e-25 99.4
MS.gene99096.t1 AT5G06320 35.542 166 100 5 75 236 48 210 3.04e-24 97.4
MS.gene99096.t1 AT2G35980 35.260 173 103 5 65 232 31 199 2.02e-22 92.4
MS.gene99096.t1 AT2G35460 31.325 166 107 4 75 236 54 216 5.90e-21 89.0
MS.gene99096.t1 AT3G52470 29.050 179 124 3 77 254 22 198 8.77e-17 77.0
MS.gene99096.t1 AT5G22870 27.778 180 127 2 73 252 27 203 9.15e-17 77.0
MS.gene99096.t1 AT3G11660 26.087 184 134 1 75 256 18 201 6.43e-16 74.7
MS.gene99096.t1 AT4G09590 29.787 188 120 4 73 254 20 201 8.50e-16 74.3
MS.gene99096.t1 AT2G35970 29.787 188 120 4 73 254 20 201 5.42e-15 72.0
MS.gene99096.t1 AT2G35960 28.488 172 111 4 89 254 35 200 2.08e-14 70.5
MS.gene99096.t1 AT2G22180 27.094 203 123 5 60 257 108 290 3.43e-14 71.2
MS.gene99096.t1 AT1G17620 25.521 192 119 4 59 232 43 228 3.86e-13 67.8
MS.gene99096.t1 AT3G44220 29.032 155 104 1 84 232 23 177 6.56e-13 66.2
MS.gene99096.t1 AT5G53730 27.389 157 106 3 85 236 37 190 2.90e-12 64.7
MS.gene99096.t1 AT5G06330 28.082 146 103 1 94 237 39 184 5.37e-12 63.9
MS.gene99096.t1 AT4G05220 24.762 210 129 8 48 236 5 206 4.58e-11 61.6

Find 44 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACCCTGAAATTAATTT+TGG 0.050421 5.2:-64126859 None:intergenic
AGTTCATCGACCGAGTATTT+TGG 0.182422 5.2:-64126492 None:intergenic
CTAATAATTCTATTAGAATT+AGG 0.234955 5.2:+64126910 MS.gene99096:CDS
GGAGCAGTAGTTGTAAATTT+AGG 0.237234 5.2:+64126931 MS.gene99096:CDS
TTGTGACCTTGATAGAATTT+AGG 0.240879 5.2:-64126678 None:intergenic
AGAAATCCAAACAAGAAAAT+TGG 0.264628 5.2:+64126585 MS.gene99096:CDS
AGCTTCCCAAAATTAATTTC+AGG 0.315887 5.2:+64126853 MS.gene99096:CDS
GGAGGACCATGTTGTTGTTC+AGG 0.329559 5.2:-64126291 None:intergenic
CCAAAATTAATTTCAGGGTT+AGG 0.341912 5.2:+64126859 MS.gene99096:CDS
TTTGATTGATTAATAGGAAT+AGG 0.373477 5.2:-64126342 None:intergenic
GGCAAAGTTTGTTGTGTACT+AGG 0.373858 5.2:-64126321 None:intergenic
GACATGTTTCTTGGTACTAA+AGG 0.406371 5.2:-64126255 None:intergenic
TGTTTCACCTTCAATTTAAG+AGG 0.407730 5.2:-64126801 None:intergenic
GTAGTTGTAAATTTAGGTTT+AGG 0.411847 5.2:+64126937 MS.gene99096:CDS
GTTGCTGCAAATTCTTCTGT+TGG 0.423745 5.2:+64126388 MS.gene99096:CDS
ATAGCACTTGGCATAACAAT+TGG 0.433295 5.2:+64126438 MS.gene99096:CDS
GTTCTTAGTATACCTTTGAC+AGG 0.440681 5.2:+64126711 MS.gene99096:CDS
GTTTGTTGTGTACTAGGAAA+AGG 0.446325 5.2:-64126315 None:intergenic
GCTTCCCAAAATTAATTTCA+GGG 0.450968 5.2:+64126854 MS.gene99096:CDS
TCTTTCAAACAACAACAGTT+TGG 0.462291 5.2:+64126533 MS.gene99096:CDS
TAGTGTTCCTCTTAAATTGA+AGG 0.472711 5.2:+64126794 MS.gene99096:CDS
GGACAAACACAGAATGCAAC+AGG 0.474658 5.2:+64126732 MS.gene99096:CDS
TTCCCAAGCTTAATCCTCAC+AGG 0.495620 5.2:-64126825 None:intergenic
CAGGGTTAGGTGTACGATTG+TGG 0.496478 5.2:+64126872 MS.gene99096:CDS
AAAATTGGAATTGACTATAG+AGG 0.508164 5.2:+64126600 MS.gene99096:CDS
TTCTGTGTTTGTCCTGTCAA+AGG 0.510330 5.2:-64126723 None:intergenic
CTTGGTACTAAAGGTGCTGA+AGG 0.518344 5.2:-64126246 None:intergenic
AACCTGTGAGGATTAAGCTT+GGG 0.519369 5.2:+64126823 MS.gene99096:CDS
TGTTGTGTACTAGGAAAAGG+AGG 0.521253 5.2:-64126312 None:intergenic
TCATTACCTAAATTCTATCA+AGG 0.551476 5.2:+64126672 MS.gene99096:CDS
CAACCTGTGAGGATTAAGCT+TGG 0.558142 5.2:+64126822 MS.gene99096:CDS
GTGGAAGTCACATAAGTGCT+TGG 0.572955 5.2:+64126622 MS.gene99096:CDS
CAGACACAAAATTATGTGAA+GGG 0.588380 5.2:+64126649 MS.gene99096:CDS
ACCAAAGATTCCAAAATACT+CGG 0.589951 5.2:+64126482 MS.gene99096:CDS
ATTGGAATTGACTATAGAGG+TGG 0.594568 5.2:+64126603 MS.gene99096:CDS
ACAGACACAAAATTATGTGA+AGG 0.595482 5.2:+64126648 MS.gene99096:CDS
GGTGCTGAAGGTTGTTGTTG+AGG 0.603656 5.2:-64126234 None:intergenic
CAGCAACAAGTTCAATCAGA+GGG 0.624043 5.2:+64126771 MS.gene99096:CDS
GCAGCAACAAGTTCAATCAG+AGG 0.642123 5.2:+64126770 MS.gene99096:CDS
GGTTAGGTGTACGATTGTGG+TGG 0.648867 5.2:+64126875 MS.gene99096:CDS
GGTGCTTCAACATCATGAAG+TGG 0.676498 5.2:-64126213 None:intergenic
TGTGTACTAGGAAAAGGAGG+AGG 0.681120 5.2:-64126309 None:intergenic
TGAAGGTGAAACAACCTGTG+AGG 0.706368 5.2:+64126811 MS.gene99096:CDS
GATGATCCTGAACAACAACA+TGG 0.735600 5.2:+64126285 MS.gene99096:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATAATTCTATTAGAATT+AGG + chr5.2:64126910-64126929 MS.gene99096:CDS 15.0%
!! TTTGATTGATTAATAGGAAT+AGG - chr5.2:64126345-64126364 None:intergenic 20.0%
! AAAATTGGAATTGACTATAG+AGG + chr5.2:64126600-64126619 MS.gene99096:CDS 25.0%
! AGAAATCCAAACAAGAAAAT+TGG + chr5.2:64126585-64126604 MS.gene99096:CDS 25.0%
! TCATTACCTAAATTCTATCA+AGG + chr5.2:64126672-64126691 MS.gene99096:CDS 25.0%
!! CTAACCCTGAAATTAATTTT+GGG - chr5.2:64126861-64126880 None:intergenic 25.0%
!! GTAGTTGTAAATTTAGGTTT+AGG + chr5.2:64126937-64126956 MS.gene99096:CDS 25.0%
!! TCAATTCCAATTTTCTTGTT+TGG - chr5.2:64126594-64126613 None:intergenic 25.0%
!!! ATATTTTTGATCATAGCACT+TGG + chr5.2:64126426-64126445 MS.gene99096:CDS 25.0%
ACAGACACAAAATTATGTGA+AGG + chr5.2:64126648-64126667 MS.gene99096:CDS 30.0%
ACCAAAGATTCCAAAATACT+CGG + chr5.2:64126482-64126501 MS.gene99096:CDS 30.0%
AGCTTCCCAAAATTAATTTC+AGG + chr5.2:64126853-64126872 MS.gene99096:CDS 30.0%
CAGACACAAAATTATGTGAA+GGG + chr5.2:64126649-64126668 MS.gene99096:CDS 30.0%
CCAAAATTAATTTCAGGGTT+AGG + chr5.2:64126859-64126878 MS.gene99096:CDS 30.0%
GAATCTTTGGTCTAAAAACA+AGG - chr5.2:64126473-64126492 None:intergenic 30.0%
GCTTCCCAAAATTAATTTCA+GGG + chr5.2:64126854-64126873 MS.gene99096:CDS 30.0%
TAGTGTTCCTCTTAAATTGA+AGG + chr5.2:64126794-64126813 MS.gene99096:CDS 30.0%
TCTTTCAAACAACAACAGTT+TGG + chr5.2:64126533-64126552 MS.gene99096:CDS 30.0%
TGTTTCACCTTCAATTTAAG+AGG - chr5.2:64126804-64126823 None:intergenic 30.0%
TTGTGACCTTGATAGAATTT+AGG - chr5.2:64126681-64126700 None:intergenic 30.0%
! CCTAACCCTGAAATTAATTT+TGG - chr5.2:64126862-64126881 None:intergenic 30.0%
!! TCAGATTTTGACATGTTTCT+TGG - chr5.2:64126267-64126286 None:intergenic 30.0%
!!! GGTGGTTTTGATTGATTAAT+AGG - chr5.2:64126351-64126370 None:intergenic 30.0%
ATAGCACTTGGCATAACAAT+TGG + chr5.2:64126438-64126457 MS.gene99096:CDS 35.0%
ATTGGAATTGACTATAGAGG+TGG + chr5.2:64126603-64126622 MS.gene99096:CDS 35.0%
GACATGTTTCTTGGTACTAA+AGG - chr5.2:64126258-64126277 None:intergenic 35.0%
GGAGCAGTAGTTGTAAATTT+AGG + chr5.2:64126931-64126950 MS.gene99096:CDS 35.0%
GTTCTTAGTATACCTTTGAC+AGG + chr5.2:64126711-64126730 MS.gene99096:CDS 35.0%
! CAACAGCTTCTTCTTTTCTT+TGG - chr5.2:64126372-64126391 None:intergenic 35.0%
!! GTTTGTTGTGTACTAGGAAA+AGG - chr5.2:64126318-64126337 None:intergenic 35.0%
!!! ACCGAGTATTTTGGAATCTT+TGG - chr5.2:64126486-64126505 None:intergenic 35.0%
AACCTGTGAGGATTAAGCTT+GGG + chr5.2:64126823-64126842 MS.gene99096:CDS 40.0%
CAGCAACAAGTTCAATCAGA+GGG + chr5.2:64126771-64126790 MS.gene99096:CDS 40.0%
GATGATCCTGAACAACAACA+TGG + chr5.2:64126285-64126304 MS.gene99096:CDS 40.0%
GTTGCTGCAAATTCTTCTGT+TGG + chr5.2:64126388-64126407 MS.gene99096:CDS 40.0%
TGTTGTGTACTAGGAAAAGG+AGG - chr5.2:64126315-64126334 None:intergenic 40.0%
TTCTGTGTTTGTCCTGTCAA+AGG - chr5.2:64126726-64126745 None:intergenic 40.0%
! AGTTCATCGACCGAGTATTT+TGG - chr5.2:64126495-64126514 None:intergenic 40.0%
! CAGCTTCTTCTTTTCTTTGG+TGG - chr5.2:64126369-64126388 None:intergenic 40.0%
!! GGCAAAGTTTGTTGTGTACT+AGG - chr5.2:64126324-64126343 None:intergenic 40.0%
CAACCTGTGAGGATTAAGCT+TGG + chr5.2:64126822-64126841 MS.gene99096:CDS 45.0%
GCAGCAACAAGTTCAATCAG+AGG + chr5.2:64126770-64126789 MS.gene99096:CDS 45.0%
GGACAAACACAGAATGCAAC+AGG + chr5.2:64126732-64126751 MS.gene99096:CDS 45.0%
GGTGCTTCAACATCATGAAG+TGG - chr5.2:64126216-64126235 None:intergenic 45.0%
GTGGAAGTCACATAAGTGCT+TGG + chr5.2:64126622-64126641 MS.gene99096:CDS 45.0%
TGAAGGTGAAACAACCTGTG+AGG + chr5.2:64126811-64126830 MS.gene99096:CDS 45.0%
TGTGTACTAGGAAAAGGAGG+AGG - chr5.2:64126312-64126331 None:intergenic 45.0%
TTCCCAAGCTTAATCCTCAC+AGG - chr5.2:64126828-64126847 None:intergenic 45.0%
!! CTTGGTACTAAAGGTGCTGA+AGG - chr5.2:64126249-64126268 None:intergenic 45.0%
CAGGGTTAGGTGTACGATTG+TGG + chr5.2:64126872-64126891 MS.gene99096:CDS 50.0%
GGTTAGGTGTACGATTGTGG+TGG + chr5.2:64126875-64126894 MS.gene99096:CDS 50.0%
! GGAGGACCATGTTGTTGTTC+AGG - chr5.2:64126294-64126313 None:intergenic 50.0%
! GGTGCTGAAGGTTGTTGTTG+AGG - chr5.2:64126237-64126256 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 64126189 64126965 64126189 ID=MS.gene99096
chr5.2 mRNA 64126189 64126965 64126189 ID=MS.gene99096.t1;Parent=MS.gene99096
chr5.2 exon 64126189 64126965 64126189 ID=MS.gene99096.t1.exon1;Parent=MS.gene99096.t1
chr5.2 CDS 64126189 64126965 64126189 ID=cds.MS.gene99096.t1;Parent=MS.gene99096.t1
Gene Sequence

>MS.gene99096

ATGGCAGATAATCAAAGAATTCATCCACTTCATGATGTTGAAGCACCTCAACAACAACCTTCAGCACCTTTAGTACCAAGAAACATGTCAAAATCTGATGATCCTGAACAACAACATGGTCCTCCTCCTTTTCCTAGTACACAACAAACTTTGCCTATTCCTATTAATCAATCAAAACCACCAAAGAAAAGAAGAAGCTGTTGCTGCAAATTCTTCTGTTGGATTTTCAGCATACTAATATTTTTGATCATAGCACTTGGCATAACAATTGGAATACTCTTCCTTGTTTTTAGACCAAAGATTCCAAAATACTCGGTCGATGAACTACGTGTCACGCAATTCGATCTTTCAAACAACAACAGTTTGGCAGTCACATTCAACTTAACATTCACTGCAAGAAATCCAAACAAGAAAATTGGAATTGACTATAGAGGTGGAAGTCACATAAGTGCTTGGTATACAGACACAAAATTATGTGAAGGGTCATTACCTAAATTCTATCAAGGTCACAAAAATGTTACTGTTCTTAGTATACCTTTGACAGGACAAACACAGAATGCAACAGGTTTGAGAGATTCATTGCAGCAACAAGTTCAATCAGAGGGTAGTGTTCCTCTTAAATTGAAGGTGAAACAACCTGTGAGGATTAAGCTTGGGAAATTGAAGCTTCCCAAAATTAATTTCAGGGTTAGGTGTACGATTGTGGTGGATAGTCTTAGTGCTAATAATTCTATTAGAATTAGGAGCAGTAGTTGTAAATTTAGGTTTAGGCTTTGA

Protein sequence

>MS.gene99096.t1

MADNQRIHPLHDVEAPQQQPSAPLVPRNMSKSDDPEQQHGPPPFPSTQQTLPIPINQSKPPKKRRSCCCKFFCWIFSILIFLIIALGITIGILFLVFRPKIPKYSVDELRVTQFDLSNNNSLAVTFNLTFTARNPNKKIGIDYRGGSHISAWYTDTKLCEGSLPKFYQGHKNVTVLSIPLTGQTQNATGLRDSLQQQVQSEGSVPLKLKVKQPVRIKLGKLKLPKINFRVRCTIVVDSLSANNSIRIRSSSCKFRFRL