AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99859.t1 MTR_2g105570 99.043 209 2 0 1 209 343 551 6.39e-150 427
MS.gene99859.t1 MTR_4g005750 85.167 209 31 0 1 209 336 544 2.64e-119 349
MS.gene99859.t1 MTR_5g007640 73.892 203 52 1 1 203 382 583 1.95e-108 323
MS.gene99859.t1 MTR_4g128337 77.295 207 45 2 1 207 344 548 1.11e-103 310
MS.gene99859.t1 MTR_4g057730 55.135 185 82 1 23 207 359 542 9.64e-69 219
MS.gene99859.t1 MTR_3g460770 53.631 179 82 1 25 203 360 537 7.08e-67 214
MS.gene99859.t1 MTR_1g063120 58.427 178 73 1 22 199 368 544 3.19e-66 213
MS.gene99859.t1 MTR_1g026310 57.865 178 74 1 22 199 376 552 4.91e-66 213
MS.gene99859.t1 MTR_2g095510 57.062 177 75 1 23 199 356 531 1.31e-65 211
MS.gene99859.t1 MTR_3g088870 55.618 178 78 1 22 199 374 550 1.47e-63 206
MS.gene99859.t1 MTR_1g090737 55.429 175 77 1 25 199 385 558 1.57e-62 204
MS.gene99859.t1 MTR_1g063110 54.802 177 79 1 23 199 365 540 5.64e-62 202
MS.gene99859.t1 MTR_4g077900 52.486 181 85 1 25 205 297 476 3.07e-61 198
MS.gene99859.t1 MTR_3g088880 54.494 178 80 1 22 199 372 548 3.67e-61 200
MS.gene99859.t1 MTR_8g039720 53.933 178 77 2 22 199 379 551 2.74e-60 197
MS.gene99859.t1 MTR_5g027480 49.756 205 99 3 1 205 353 553 3.79e-60 197
MS.gene99859.t1 MTR_5g027500 50.244 205 98 3 1 205 366 566 7.65e-60 196
MS.gene99859.t1 MTR_1g073180 51.648 182 86 2 22 203 351 530 1.66e-59 195
MS.gene99859.t1 MTR_1g073180 50.311 161 78 2 22 182 373 531 7.77e-49 167
MS.gene99859.t1 MTR_4g057730 60.177 113 45 0 23 135 359 471 2.39e-44 154
MS.gene99859.t1 MTR_4g057730 55.435 92 41 0 23 114 359 450 4.55e-32 120
MS.gene99859.t1 MTR_3g035130 36.158 177 107 5 22 197 334 505 1.64e-30 117
MS.gene99859.t1 MTR_2g095510 56.522 92 40 0 23 114 356 447 4.78e-30 115
MS.gene99859.t1 MTR_1569s0010 36.216 185 113 4 18 200 347 528 3.46e-28 110
MS.gene99859.t1 MTR_5g076240 38.764 178 101 4 30 200 365 541 4.36e-28 110
MS.gene99859.t1 MTR_5g076220 36.994 173 103 3 33 200 371 542 1.14e-27 109
MS.gene99859.t1 MTR_3g064420 35.385 195 117 3 16 202 356 549 1.22e-27 109
MS.gene99859.t1 MTR_5g008800 37.572 173 103 4 35 205 369 538 2.29e-26 105
MS.gene99859.t1 MTR_0161s0050 35.838 173 105 4 33 200 373 544 2.63e-26 105
MS.gene99859.t1 MTR_5g008820 36.207 174 106 4 34 205 366 536 2.92e-26 105
MS.gene99859.t1 MTR_4g057755 57.534 73 31 0 62 134 46 118 8.19e-26 97.1
MS.gene99859.t1 MTR_5g076200 35.838 173 105 4 33 200 371 542 2.76e-25 102
MS.gene99859.t1 MTR_8g074040 37.013 154 94 3 52 205 314 464 1.23e-24 100
MS.gene99859.t1 MTR_8g074040 37.013 154 94 3 52 205 432 582 1.35e-24 100
MS.gene99859.t1 MTR_8g074120 34.375 192 121 4 16 205 305 493 1.38e-24 100
MS.gene99859.t1 MTR_3g035130 39.535 129 75 3 22 150 334 459 3.00e-23 96.7
MS.gene99859.t1 MTR_0803s0020 30.457 197 130 5 1 193 541 734 4.04e-22 94.0
MS.gene99859.t1 MTR_2g044180 37.430 179 98 6 29 202 294 463 4.34e-22 93.6
MS.gene99859.t1 MTR_2g044180 37.430 179 98 6 29 202 385 554 4.84e-22 93.6
MS.gene99859.t1 MTR_4g121600 35.000 180 101 6 29 202 385 554 4.61e-20 87.8
MS.gene99859.t1 MTR_4g013210 27.179 195 129 5 22 205 211 403 7.34e-18 81.3
MS.gene99859.t1 MTR_4g013210 27.179 195 129 5 22 205 252 444 8.98e-18 81.3
MS.gene99859.t1 MTR_4g013210 27.179 195 129 5 22 205 363 555 1.19e-17 80.9
MS.gene99859.t1 MTR_3g084470 29.832 238 122 9 1 202 477 705 1.70e-17 80.5
MS.gene99859.t1 MTR_8g083170 29.661 236 127 8 1 202 484 714 9.74e-17 78.6
MS.gene99859.t1 MTR_4g011790 25.405 185 131 3 22 200 409 592 1.23e-16 78.2
MS.gene99859.t1 MTR_4g011790 25.405 185 131 3 22 200 418 601 1.32e-16 77.8
MS.gene99859.t1 MTR_7g084850 30.000 170 112 4 37 200 549 717 2.21e-16 77.4
MS.gene99859.t1 MTR_3g084450 27.700 213 119 5 24 202 499 710 3.56e-16 76.6
MS.gene99859.t1 MTR_3g084450 27.700 213 119 5 24 202 291 502 3.83e-16 76.6
MS.gene99859.t1 MTR_5g023730 31.638 177 114 5 33 205 369 542 4.91e-16 76.3
MS.gene99859.t1 MTR_4g011790 25.532 188 133 3 22 203 409 595 1.38e-15 75.1
MS.gene99859.t1 MTR_3g088890 45.455 77 36 3 95 171 2 72 6.25e-14 65.1
MS.gene99859.t1 MTR_4g013210 29.008 131 89 3 22 150 404 532 1.69e-13 68.9
MS.gene99859.t1 MTR_2g030240 27.149 221 137 6 2 207 242 453 7.46e-13 67.0
MS.gene99859.t1 MTR_1g104810 41.176 102 54 4 23 119 464 564 8.22e-13 67.0
MS.gene99859.t1 MTR_2g030240 27.149 221 137 6 2 207 336 547 9.59e-13 66.6
MS.gene99859.t1 MTR_1g104810 41.176 102 54 4 23 119 397 497 9.87e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99859.t1 AT3G21240 78.261 207 44 1 1 207 349 554 2.07e-115 340
MS.gene99859.t1 AT1G51680 78.744 207 43 1 1 207 356 561 1.24e-105 315
MS.gene99859.t1 AT1G65060 72.059 204 56 1 1 204 359 561 2.50e-104 311
MS.gene99859.t1 AT3G21230 73.399 203 52 2 1 203 364 564 5.91e-103 308
MS.gene99859.t1 AT3G21230 70.330 182 48 3 1 182 364 539 8.01e-85 261
MS.gene99859.t1 AT1G51680 78.363 171 36 1 1 171 356 525 1.79e-82 254
MS.gene99859.t1 AT3G21240 81.343 134 25 0 1 134 349 482 4.83e-75 234
MS.gene99859.t1 AT3G21240 81.343 134 25 0 1 134 349 482 4.83e-75 234
MS.gene99859.t1 AT3G21230 76.119 134 31 1 1 134 364 496 1.02e-68 218
MS.gene99859.t1 AT4G05160 57.865 178 74 1 22 199 360 536 4.73e-68 218
MS.gene99859.t1 AT1G20510 57.303 178 75 1 22 199 360 536 3.28e-67 215
MS.gene99859.t1 AT1G20480 55.612 196 82 2 8 199 363 557 7.58e-67 215
MS.gene99859.t1 AT1G51680 82.836 134 23 0 1 134 356 489 7.58e-66 210
MS.gene99859.t1 AT5G63380 55.866 179 78 1 21 199 373 550 3.18e-65 211
MS.gene99859.t1 AT1G20500 56.180 178 77 1 22 199 366 542 1.03e-64 209
MS.gene99859.t1 AT1G62940 56.044 182 79 1 22 203 356 536 5.09e-61 199
MS.gene99859.t1 AT5G38120 55.618 178 75 2 22 199 369 542 1.92e-59 195
MS.gene99859.t1 AT4G19010 47.343 207 105 3 1 207 360 562 4.41e-58 192
MS.gene99859.t1 AT1G65060 73.913 115 30 0 1 115 359 473 4.93e-56 185
MS.gene99859.t1 AT5G38120 52.980 151 67 2 22 172 369 515 1.75e-45 157
MS.gene99859.t1 AT1G20510 60.526 114 45 0 22 135 360 473 6.95e-43 150
MS.gene99859.t1 AT5G38120 57.895 114 45 1 22 135 369 479 5.81e-37 134
MS.gene99859.t1 AT5G38120 57.895 114 45 1 22 135 369 479 5.81e-37 134
MS.gene99859.t1 AT5G16340 40.351 171 98 3 33 200 369 538 1.86e-32 123
MS.gene99859.t1 AT3G48990 39.773 176 102 4 22 197 333 504 2.16e-32 122
MS.gene99859.t1 AT5G16370 39.766 171 99 3 33 200 369 538 1.78e-31 120
MS.gene99859.t1 AT1G21540 37.569 181 107 3 33 208 371 550 2.11e-30 117
MS.gene99859.t1 AT1G77240 37.079 178 109 2 33 208 369 545 2.66e-30 117
MS.gene99859.t1 AT1G68270 38.122 181 98 5 33 201 342 520 3.36e-29 114
MS.gene99859.t1 AT1G75960 38.596 171 101 3 33 200 369 538 3.59e-29 114
MS.gene99859.t1 AT1G20510 55.435 92 41 0 22 113 360 451 1.31e-28 111
MS.gene99859.t1 AT1G65880 37.222 180 100 4 33 200 372 550 8.80e-28 110
MS.gene99859.t1 AT1G21530 36.000 175 105 2 33 200 366 540 2.73e-27 108
MS.gene99859.t1 AT1G21530 36.000 175 105 2 33 200 368 542 2.95e-27 108
MS.gene99859.t1 AT1G65890 34.703 219 122 8 1 200 333 549 1.23e-26 107
MS.gene99859.t1 AT1G66120 36.517 178 105 4 30 200 366 542 2.91e-24 100
MS.gene99859.t1 AT1G20560 33.523 176 112 4 34 207 296 468 1.51e-23 97.8
MS.gene99859.t1 AT1G20560 33.523 176 112 4 34 207 374 546 1.78e-23 97.8
MS.gene99859.t1 AT3G21230 70.312 64 18 1 1 64 364 426 2.88e-23 96.7
MS.gene99859.t1 AT3G16910 34.659 176 105 5 34 202 385 557 6.15e-23 96.3
MS.gene99859.t1 AT1G76290 36.047 172 103 5 37 204 366 534 2.17e-21 91.7
MS.gene99859.t1 AT2G17650 32.353 170 111 3 35 202 429 596 2.40e-21 91.7
MS.gene99859.t1 AT3G16170 26.562 192 127 3 22 203 417 604 1.38e-18 84.0
MS.gene99859.t1 AT5G36880 28.235 170 115 4 37 200 494 662 1.66e-15 74.7
MS.gene99859.t1 AT5G36880 28.235 170 115 4 37 200 494 662 1.66e-15 74.7
MS.gene99859.t1 AT5G36880 28.235 170 115 4 37 200 494 662 1.66e-15 74.7
MS.gene99859.t1 AT5G36880 28.235 170 115 4 37 200 494 662 1.66e-15 74.7
MS.gene99859.t1 AT5G36880 28.235 170 115 4 37 200 544 712 1.72e-15 74.7
MS.gene99859.t1 AT1G30520 31.176 170 99 4 52 203 380 549 3.75e-14 70.9
MS.gene99859.t1 AT1G30520 31.176 170 99 4 52 203 380 549 3.75e-14 70.9
MS.gene99859.t1 AT1G20490 47.619 63 33 0 20 82 366 428 9.50e-13 66.6
MS.gene99859.t1 AT1G20490 47.619 63 33 0 20 82 366 428 9.50e-13 66.6
MS.gene99859.t1 AT5G27600 39.423 104 53 4 23 119 467 567 1.59e-12 66.2
MS.gene99859.t1 AT3G23790 38.889 90 50 2 1 90 482 566 1.62e-12 66.2
MS.gene99859.t1 AT4G14070 38.710 93 46 3 1 90 498 582 2.14e-12 65.9
MS.gene99859.t1 AT3G05970 37.624 101 59 2 23 119 467 567 3.38e-11 62.4

Find 36 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGCAGCATCAGAAATTT+TGG 0.154865 2.1:+297303 None:intergenic
CAACCAACCTTCTTTGTCTA+TGG 0.233625 2.1:+297456 None:intergenic
ATACATCACCTTTCATGATT+TGG 0.282523 2.1:+298878 None:intergenic
TGGTTGTATACAGGTGATAT+TGG 0.318824 2.1:-297439 MS.gene99859:CDS
GGTTGTATACAGGTGATATT+GGG 0.389319 2.1:-297438 MS.gene99859:CDS
AGAGGTACCTGTTGCATTTG+TGG 0.414419 2.1:-297129 MS.gene99859:CDS
TAAGCACTTTATCTCCAAAC+AGG 0.437851 2.1:-297066 MS.gene99859:intron
TTATGCAGATTTCACCAGAT+TGG 0.438572 2.1:+298908 None:intergenic
ATTCATTGTTGATAGATTGA+AGG 0.440963 2.1:-297392 MS.gene99859:CDS
AGAAGAGCTTCAAGTTCAGC+TGG 0.466302 2.1:+297337 None:intergenic
TCTTTACCTCGAAACCAATC+TGG 0.470219 2.1:-298922 MS.gene99859:CDS
GGTAAAGATTTACCAGTGTC+AGG 0.475114 2.1:+298937 None:intergenic
TCTGGTGAAATCTGCATAAG+AGG 0.475587 2.1:-298904 MS.gene99859:CDS
GCAATTCCCAAGTCACCATC+AGG 0.482425 2.1:-296691 MS.gene99859:CDS
ATTTCACCAGATTGGTTTCG+AGG 0.484356 2.1:+298916 None:intergenic
GCATTTGTGGTGAGATCAAA+TGG 0.485996 2.1:-297116 MS.gene99859:CDS
GATCTCACCACAAATGCAAC+AGG 0.486516 2.1:+297122 None:intergenic
AAAGAAGGTTGGTTGTATAC+AGG 0.501330 2.1:-297448 MS.gene99859:CDS
GGATACGGAATGACTGAGGC+GGG 0.504464 2.1:-299063 None:intergenic
TTGAGAGCAAAGCTAGCAGC+AGG 0.532670 2.1:-296649 MS.gene99859:CDS
GAAGGATGAAGCGGCTGGAG+AGG 0.557352 2.1:-297147 MS.gene99859:CDS
AAATGCAGAGATGAAGATTG+TGG 0.577117 2.1:-298962 MS.gene99859:CDS
GAACCAGTGAATGTAAAAGC+AGG 0.579482 2.1:-299006 MS.gene99859:CDS
GAGAGAACCATAGACAAAGA+AGG 0.579648 2.1:-297463 MS.gene99859:CDS
AGAATGAAGGATGAAGCGGC+TGG 0.583246 2.1:-297152 MS.gene99859:CDS
ATCAGGCAAAATCTTGCGCA+AGG 0.592909 2.1:-296674 MS.gene99859:CDS
AAGGAATTAATCAAATACAA+AGG 0.598333 2.1:-297373 MS.gene99859:CDS
AAGATTGTGGATCCTGACAC+TGG 0.615148 2.1:-298949 MS.gene99859:CDS
GAACCATAGACAAAGAAGGT+TGG 0.619000 2.1:-297459 MS.gene99859:CDS
AATGTAAAAGCAGGTGCATG+TGG 0.625412 2.1:-298997 MS.gene99859:CDS
AGAGGCGACCAAATCATGAA+AGG 0.629028 2.1:-298886 MS.gene99859:intron
GCTAAACTCATCGTTAACAC+TGG 0.639259 2.1:+299039 None:intergenic
GGGATACGGAATGACTGAGG+CGG 0.641098 2.1:-299064 None:intergenic
GATACGGAATGACTGAGGCG+GGG 0.645430 2.1:-299062 None:intergenic
AGGTTATCTAAATGATCGAG+AGG 0.669178 2.1:-297491 MS.gene99859:CDS
ACAGGGATACGGAATGACTG+AGG 0.707818 2.1:-299067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGAATTAATAATATGAA+TGG + chr2.1:298451-298470 None:intergenic 10.0%
!! TTAATTAAGTAAAGTATATT+AGG + chr2.1:296996-297015 None:intergenic 10.0%
!! TTGTAAAATATAATTAATAC+AGG + chr2.1:297660-297679 None:intergenic 10.0%
!!! AAAAAATAGATTTTTTTTAC+AGG + chr2.1:297439-297458 None:intergenic 10.0%
!!! AAATTTAAATTTTAGAATGA+AGG - chr2.1:298525-298544 MS.gene99859:intron 10.0%
!! CAAATTAATGTTAATAATTG+AGG - chr2.1:298913-298932 MS.gene99859:CDS 15.0%
!! GAAAAACAAACTATATAAAA+TGG - chr2.1:297375-297394 MS.gene99859:CDS 15.0%
!! GGAAAAATTGATTAAATATA+AGG - chr2.1:298761-298780 MS.gene99859:intron 15.0%
!!! ACATGAAAATACATAATTTT+TGG - chr2.1:298671-298690 MS.gene99859:intron 15.0%
!!! ATTTTCATGTAAAAGTTAAA+AGG + chr2.1:298661-298680 None:intergenic 15.0%
!!! CATGAAAATACATAATTTTT+GGG - chr2.1:298672-298691 MS.gene99859:intron 15.0%
!!! TTAATCTCGTTTTTAATAAT+AGG - chr2.1:296935-296954 MS.gene99859:intron 15.0%
!! AAATGATTAAATTTGGAAAG+TGG + chr2.1:296865-296884 None:intergenic 20.0%
!! AAGAAGGAAATGATTAAATT+TGG + chr2.1:296872-296891 None:intergenic 20.0%
!! AAGGAATTAATCAAATACAA+AGG - chr2.1:298317-298336 MS.gene99859:intron 20.0%
!! ATTAAATAATGAAATGATGC+AGG - chr2.1:298179-298198 MS.gene99859:intron 20.0%
!! CTTAAAAAAGCTATAACAAA+CGG - chr2.1:297590-297609 MS.gene99859:intron 20.0%
!! GAGTAAAAATTGACTAAATT+AGG - chr2.1:297296-297315 MS.gene99859:intron 20.0%
!! TAACAAAACAAAAAGTTAGT+AGG - chr2.1:298740-298759 MS.gene99859:intron 20.0%
!! TTAAATTATCTCATCCAAAT+TGG - chr2.1:297160-297179 MS.gene99859:intron 20.0%
!! TTTGGATGAGATAATTTAAA+AGG + chr2.1:297159-297178 None:intergenic 20.0%
!!! ATAGTTTGTTTTTCTTCAAA+AGG + chr2.1:297369-297388 None:intergenic 20.0%
!!! CGTTTTTAATAATAGGAAAA+GGG - chr2.1:296942-296961 MS.gene99859:intron 20.0%
!!! GTTTTTAATAATAGGAAAAG+GGG - chr2.1:296943-296962 MS.gene99859:intron 20.0%
!!! TCGTTTTTAATAATAGGAAA+AGG - chr2.1:296941-296960 MS.gene99859:intron 20.0%
! AAATGTACTTTAAATCCACT+TGG + chr2.1:297246-297265 None:intergenic 25.0%
! AATGTACTTTAAATCCACTT+GGG + chr2.1:297245-297264 None:intergenic 25.0%
! ATAAGAAAAGACTAGACTTA+CGG + chr2.1:298416-298435 None:intergenic 25.0%
! GTTATTACATTACATGAAAG+TGG + chr2.1:298058-298077 None:intergenic 25.0%
! TTAGCCAATTTGGATAAATT+AGG - chr2.1:297795-297814 MS.gene99859:intron 25.0%
!! ATGTTAATAATTGAGGTGTT+TGG - chr2.1:298920-298939 MS.gene99859:CDS 25.0%
!! ATTATATTTTACAACTGCTC+TGG - chr2.1:297666-297685 MS.gene99859:intron 25.0%
!! ATTCATTGTTGATAGATTGA+AGG - chr2.1:298298-298317 MS.gene99859:intron 25.0%
!! GAGAAGTACATACATAAATT+AGG + chr2.1:298842-298861 None:intergenic 25.0%
!! TCAATTTTTACTCGTTCTAA+AGG + chr2.1:297289-297308 None:intergenic 25.0%
!! TGTTAATAATTGAGGTGTTT+GGG - chr2.1:298921-298940 MS.gene99859:CDS 25.0%
!!! AAAGCCTTATTTATACCTTT+GGG + chr2.1:297625-297644 None:intergenic 25.0%
!!! ATGACACAAGAAGTATTTAA+GGG - chr2.1:297910-297929 MS.gene99859:intron 25.0%
!!! CTTTGAAATATTGGAAACAA+AGG - chr2.1:297744-297763 MS.gene99859:intron 25.0%
!!! GAAAATACATAATTTTTGGG+AGG - chr2.1:298675-298694 MS.gene99859:intron 25.0%
!!! GTGTCTACATACATTTTTTT+TGG + chr2.1:298080-298099 None:intergenic 25.0%
!!! TCAAAAGGTGTTTATAAAAG+AGG + chr2.1:297354-297373 None:intergenic 25.0%
AAATGATTGATTGCAAAGGT+AGG - chr2.1:297977-297996 MS.gene99859:intron 30.0%
AACTACACGCTTTCAATATT+CGG + chr2.1:297939-297958 None:intergenic 30.0%
ACATAAATTAGGTGACAAGT+AGG + chr2.1:298831-298850 None:intergenic 30.0%
ATACATCACCTTTCATGATT+TGG + chr2.1:296815-296834 None:intergenic 30.0%
ATGTGCATAATGTTTGACAA+CGG - chr2.1:297105-297124 MS.gene99859:CDS 30.0%
CATGAAATGATTGATTGCAA+AGG - chr2.1:297973-297992 MS.gene99859:intron 30.0%
GTTTATAAAAGAGGCGAAAT+GGG + chr2.1:297345-297364 None:intergenic 30.0%
TAAGAAAAGACTAGACTTAC+GGG + chr2.1:298415-298434 None:intergenic 30.0%
TGTGCCTAATTTATCCAAAT+TGG + chr2.1:297802-297821 None:intergenic 30.0%
TGTTTATAAAAGAGGCGAAA+TGG + chr2.1:297346-297365 None:intergenic 30.0%
! AAGTCCCAAAGGTATAAATA+AGG - chr2.1:297618-297637 MS.gene99859:intron 30.0%
! AATATTTCAAAGTGCTAGCT+TGG + chr2.1:297736-297755 None:intergenic 30.0%
! GAAACAAAGGTTCTTTTTAG+AGG - chr2.1:297757-297776 MS.gene99859:intron 30.0%
!! AAGCTAGCACTTTGAAATAT+TGG - chr2.1:297735-297754 MS.gene99859:intron 30.0%
!! CATGACACAAGAAGTATTTA+AGG - chr2.1:297909-297928 MS.gene99859:intron 30.0%
!! GAAAGCCTTATTTATACCTT+TGG + chr2.1:297626-297645 None:intergenic 30.0%
!! TACATAATTTTTGGGAGGTA+AGG - chr2.1:298680-298699 MS.gene99859:intron 30.0%
!!! TTTTAGAATGAAGGATGAAG+CGG - chr2.1:298534-298553 MS.gene99859:intron 30.0%
AAATGCAGAGATGAAGATTG+TGG - chr2.1:296728-296747 MS.gene99859:CDS 35.0%
AGGAAGAAAAAGAACCTGTT+TGG + chr2.1:298641-298660 None:intergenic 35.0%
AGGTTATCTAAATGATCGAG+AGG - chr2.1:298199-298218 MS.gene99859:intron 35.0%
ATAAATAAGGCTTTCGCTGA+CGG - chr2.1:297631-297650 MS.gene99859:intron 35.0%
ATACCTCACACACAACATTT+AGG + chr2.1:298866-298885 None:intergenic 35.0%
CAACGGTATATATGAGAATG+AGG - chr2.1:297122-297141 MS.gene99859:CDS 35.0%
GGTTGTATACAGGTGATATT+GGG - chr2.1:298252-298271 MS.gene99859:intron 35.0%
TAAGCACTTTATCTCCAAAC+AGG - chr2.1:298624-298643 MS.gene99859:intron 35.0%
TATGCATATGCATGCATGAA+TGG + chr2.1:298506-298525 None:intergenic 35.0%
TGGTTGTATACAGGTGATAT+TGG - chr2.1:298251-298270 MS.gene99859:intron 35.0%
TGTGAGTTAAGATCTTAGAG+CGG - chr2.1:298793-298812 MS.gene99859:intron 35.0%
TTATGCAGATTTCACCAGAT+TGG + chr2.1:296785-296804 None:intergenic 35.0%
! AAAGAAGGTTGGTTGTATAC+AGG - chr2.1:298242-298261 MS.gene99859:intron 35.0%
! AACAGCAGCATCAGAAATTT+TGG + chr2.1:298390-298409 None:intergenic 35.0%
! ACAGCAGCATCAGAAATTTT+GGG + chr2.1:298389-298408 None:intergenic 35.0%
! GAAATCAAGGTTAGCCAATT+TGG - chr2.1:297785-297804 MS.gene99859:intron 35.0%
! GTGTTTGGGTTTGATATTAC+AGG - chr2.1:298935-298954 MS.gene99859:CDS 35.0%
! TTTGGGTTTGATATTACAGG+TGG - chr2.1:298938-298957 MS.gene99859:CDS 35.0%
AATGTAAAAGCAGGTGCATG+TGG - chr2.1:296693-296712 MS.gene99859:CDS 40.0%
ACATTACATGAAAGTGGAGC+TGG + chr2.1:298052-298071 None:intergenic 40.0%
CAACCAACCTTCTTTGTCTA+TGG + chr2.1:298237-298256 None:intergenic 40.0%
GAACCAGTGAATGTAAAAGC+AGG - chr2.1:296684-296703 MS.gene99859:CDS 40.0%
GAACCATAGACAAAGAAGGT+TGG - chr2.1:298231-298250 MS.gene99859:intron 40.0%
GAGAGAACCATAGACAAAGA+AGG - chr2.1:298227-298246 MS.gene99859:intron 40.0%
GCATTTGTGGTGAGATCAAA+TGG - chr2.1:298574-298593 MS.gene99859:intron 40.0%
GCTAAACTCATCGTTAACAC+TGG + chr2.1:296654-296673 None:intergenic 40.0%
GGTAAAGATTTACCAGTGTC+AGG + chr2.1:296756-296775 None:intergenic 40.0%
GGTCTCAAACCAATATCATC+AGG - chr2.1:297215-297234 MS.gene99859:intron 40.0%
GTGAGTTAAGATCTTAGAGC+GGG - chr2.1:298794-298813 MS.gene99859:intron 40.0%
TCACCTAAATGTTGTGTGTG+AGG - chr2.1:298860-298879 MS.gene99859:intron 40.0%
TCTGGTGAAATCTGCATAAG+AGG - chr2.1:296786-296805 MS.gene99859:intron 40.0%
TCTTTACCTCGAAACCAATC+TGG - chr2.1:296768-296787 MS.gene99859:intron 40.0%
! GATTTCTTGTGGTGCCAATT+TGG + chr2.1:297177-297196 None:intergenic 40.0%
! TTGAAGTGTCCTCTCTTCAT+GGG - chr2.1:298000-298019 MS.gene99859:intron 40.0%
! TTTAGAGGCGTGAGAAATCA+AGG - chr2.1:297772-297791 MS.gene99859:intron 40.0%
!! ATTTCACCAGATTGGTTTCG+AGG + chr2.1:296777-296796 None:intergenic 40.0%
!! ATTTTGCCTGATGGTGACTT+GGG + chr2.1:299008-299027 None:intergenic 40.0%
!! TGGAGCTGGATTGTTTTGTT+TGG + chr2.1:298038-298057 None:intergenic 40.0%
!! TTGGGTTGACCTGATGATAT+TGG + chr2.1:297227-297246 None:intergenic 40.0%
AAGATTGTGGATCCTGACAC+TGG - chr2.1:296741-296760 MS.gene99859:intron 45.0%
AGAGGCGACCAAATCATGAA+AGG - chr2.1:296804-296823 MS.gene99859:intron 45.0%
ATCAGGCAAAATCTTGCGCA+AGG - chr2.1:299016-299035 MS.gene99859:CDS 45.0%
ATCGCAGGTTCGATTTCTTG+TGG + chr2.1:297188-297207 None:intergenic 45.0%
CAACAACATCCCATGAAGAG+AGG + chr2.1:298012-298031 None:intergenic 45.0%
GATCTCACCACAAATGCAAC+AGG + chr2.1:298571-298590 None:intergenic 45.0%
TATCATCAGGTCAACCCAAG+TGG - chr2.1:297228-297247 MS.gene99859:intron 45.0%
! AAACGGACTCTAAGTCCCAA+AGG - chr2.1:297607-297626 MS.gene99859:intron 45.0%
! AGAAGAGCTTCAAGTTCAGC+TGG + chr2.1:298356-298375 None:intergenic 45.0%
! GTTGAAGTGTCCTCTCTTCA+TGG - chr2.1:297999-298018 MS.gene99859:intron 45.0%
! TTGCGCAAGATTTTGCCTGA+TGG + chr2.1:299017-299036 None:intergenic 45.0%
!! AGAGGTACCTGTTGCATTTG+TGG - chr2.1:298561-298580 MS.gene99859:intron 45.0%
!! GATTTTGCCTGATGGTGACT+TGG + chr2.1:299009-299028 None:intergenic 45.0%
!! GCACCTGCTTTTACATTCAC+TGG + chr2.1:296690-296709 None:intergenic 45.0%
!! AAATTAATTTAATATACTTT+GGG - chr2.1:296894-296913 MS.gene99859:intron 5.0%
!! ATTTATAATAACAATTTAAT+TGG + chr2.1:297874-297893 None:intergenic 5.0%
!! TAAATTAATTTAATATACTT+TGG - chr2.1:296893-296912 MS.gene99859:intron 5.0%
!! TAATTTATTAAAAAAAAGAT+TGG + chr2.1:298110-298129 None:intergenic 5.0%
!! TATTAAATTAATTTAAAAGA+AGG + chr2.1:296888-296907 None:intergenic 5.0%
AGAATGAAGGATGAAGCGGC+TGG - chr2.1:298538-298557 MS.gene99859:intron 50.0%
ATCGAACCTGCGATTTCGAG+AGG - chr2.1:297194-297213 MS.gene99859:intron 50.0%
GCAATTCCCAAGTCACCATC+AGG - chr2.1:298999-299018 MS.gene99859:CDS 50.0%
TTGAGACCTCTCGAAATCGC+AGG + chr2.1:297203-297222 None:intergenic 50.0%
TTGAGAGCAAAGCTAGCAGC+AGG - chr2.1:299041-299060 MS.gene99859:CDS 50.0%
GAAGGATGAAGCGGCTGGAG+AGG - chr2.1:298543-298562 MS.gene99859:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 296636 299076 296636 ID=MS.gene99859
chr2.1 mRNA 296636 299076 296636 ID=MS.gene99859.t1;Parent=MS.gene99859
chr2.1 exon 298887 299076 298887 ID=MS.gene99859.t1.exon1;Parent=MS.gene99859.t1
chr2.1 CDS 298887 299076 298887 ID=cds.MS.gene99859.t1;Parent=MS.gene99859.t1
chr2.1 exon 297298 297511 297298 ID=MS.gene99859.t1.exon2;Parent=MS.gene99859.t1
chr2.1 CDS 297298 297511 297298 ID=cds.MS.gene99859.t1;Parent=MS.gene99859.t1
chr2.1 exon 297067 297172 297067 ID=MS.gene99859.t1.exon3;Parent=MS.gene99859.t1
chr2.1 CDS 297067 297172 297067 ID=cds.MS.gene99859.t1;Parent=MS.gene99859.t1
chr2.1 exon 296636 296755 296636 ID=MS.gene99859.t1.exon4;Parent=MS.gene99859.t1
chr2.1 CDS 296636 296755 296636 ID=cds.MS.gene99859.t1;Parent=MS.gene99859.t1
Gene Sequence

>MS.gene99859

ATGACTGAGGCGGGGCCAGTGTTAACGATGAGTTTAGCATTTGCTAAAGAACCAGTGAATGTAAAAGCAGGTGCATGTGGAACTGTTGTAAGAAATGCAGAGATGAAGATTGTGGATCCTGACACTGGTAAATCTTTACCTCGAAACCAATCTGGTGAAATCTGCATAAGAGGCGACCAAATCATGAAAGGTGATGTATATATATCATTCTATTAACATTATTCATCCACTTTCCAAATTTAATCATTTCCTTCTTTTAAATTAATTTAATATACTTTGGGCTTTATTATAATCTTGTTTTAATCTCGTTTTTAATAATAGGAAAAGGGGTGCTATCACAGAATGCACTGTTAATCACCTAATATACTTTACTTAATTAAAATTTATCTCAATTTATCATTTTTTACACAATCACATTTCATCATATATAGAATATAAATATAAATTACTTAATAACTTTTTTGAAAAAATGTGCATAATGTTTGACAACGGTATATATGAGAATGAGGTACAAAAAGTACCTTTTAAATTATCTCATCCAAATTGGCACCACAAGAAATCGAACCTGCGATTTCGAGAGGTCTCAAACCAATATCATCAGGTCAACCCAAGTGGATTTAAAGTACATTTTACTTAATAGCTAAATCATTCCTTTAGAACGAGTAAAAATTGACTAAATTAGGAAGAAAGATAGCACTCAAAACTACCCATTTCGCCTCTTTTATAAACACCTTTTGAAGAAAAACAAACTATATAAAATGGATTTTTTTTTATAAAATTAGTTCAATTTTTTAAAAAATCCTGTAAAAAAAATCTATTTTTTGAGAAGCTGCTCTTAACAGCTTCTCATTTGAAGTTGTTTTTAGATTCTGATTTTTTTTTTAAAAAAAAATTGTAACAAACATATGCAAAACTCTTAAAAGTGATTATTTTTTTTAAAATAAGTGATTTTTTCTTAAAAAAGCTATAACAAACGGACTCTAAGTCCCAAAGGTATAAATAAGGCTTTCGCTGACGGATACCTGTATTAATTATATTTTACAACTGCTCTGGAGAGTTTGAACTCAACACATTGATATTATTAGACAAAGCTCTTACCAAGCTAGCACTTTGAAATATTGGAAACAAAGGTTCTTTTTAGAGGCGTGAGAAATCAAGGTTAGCCAATTTGGATAAATTAGGCACATTAAATAAGCTATCTATTTTATATATGTTAACACATTTAAAAGTGTTTACCAATTAAATTGTTATTATAAATTTTTATACTAGTTAACATGACACAAGAAGTATTTAAGGGTTGCCGAATATTGAAAGCGTGTAGTTAGATTAGAGCGCAACATGAAATGATTGATTGCAAAGGTAGGTTGAAGTGTCCTCTCTTCATGGGATGTTGTTGACTCCAAACAAAACAATCCAGCTCCACTTTCATGTAATGTAATAACCAAAAAAAATGTATGTAGACACATACATACCAATCTTTTTTTTAATAAATTAATTATGAAAAATTTGTATTAACTATATATGAACAAGTGAAAATTGATTAATTAAATAATGAAATGATGCAGGTTATCTAAATGATCGAGAGGCAACAGAGAGAACCATAGACAAAGAAGGTTGGTTGTATACAGGTGATATTGGGTATATTGATGAAGATGATGAGTTATTCATTGTTGATAGATTGAAGGAATTAATCAAATACAAAGGATTTCAAGTTGCTCCAGCTGAACTTGAAGCTCTTCTTCTCTCTCATCCCAAAATTTCTGATGCTGCTGTTGTCCCGTAAGTCTAGTCTTTTCTTATGCTATGAAATTTCCATTCATATTATTAATTCAATTCAATTCAATTCAATTCAATGTTTATTTATTTACCATTCATGCATGCATATGCATAAATTTAAATTTTAGAATGAAGGATGAAGCGGCTGGAGAGGTACCTGTTGCATTTGTGGTGAGATCAAATGGTTACACTGACTTAACTCAAGATGAAATTAAGCACTTTATCTCCAAACAGGTTCTTTTTCTTCCTTTTAACTTTTACATGAAAATACATAATTTTTGGGAGGTAAGGTCTCGTTGAATAAACTTAAATTGCATAATGAATTGACTAACAAAACAAAAAGTTAGTAGGAAAAATTGATTAAATATAAGGACATGAGCTTGTGAGTTAAGATCTTAGAGCGGGTGCATTAATTACCTACTTGTCACCTAATTTATGTATGTACTTCTCACCTAAATGTTGTGTGTGAGGTATAGCAACTTTCTTACACTACAAGTTTAGCAAATTAATGTTAATAATTGAGGTGTTTGGGTTTGATATTACAGGTGGTGTTTTACAAAAGAATAAATCAAGTATTCTTCATTGATGCAATTCCCAAGTCACCATCAGGCAAAATCTTGCGCAAGGATTTGAGAGCAAAGCTAGCAGCAGGTGTTCCACATTGA
Protein sequence

>MS.gene99859.t1

MTEAGPVLTMSLAFAKEPVNVKAGACGTVVRNAEMKIVDPDTGKSLPRNQSGEICIRGDQIMKGYLNDREATERTIDKEGWLYTGDIGYIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLSHPKISDAAVVPMKDEAAGEVPVAFVVRSNGYTDLTQDEIKHFISKQVVFYKRINQVFFIDAIPKSPSGKILRKDLRAKLAAGVPH