Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048321.01.T01 | XP_003605890.1 | 86.624 | 314 | 10 | 3 | 1 | 283 | 1 | 313 | 0 | 527 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048321.01.T01 | A2XQE8 | 57.237 | 304 | 95 | 6 | 12 | 283 | 9 | 309 | 2.92E-119 | 348 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048321.01.T01 | G7JVY2 | 86.624 | 314 | 10 | 3 | 1 | 283 | 1 | 313 | 0.0 | 527 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048321.01.T01 | MTR_4g047610 | 86.624 | 314 | 10 | 3 | 1 | 283 | 1 | 313 | 0.0 | 527 |
| MsG0080048321.01.T01 | MTR_5g022560 | 80.255 | 314 | 31 | 2 | 1 | 283 | 1 | 314 | 0.0 | 517 |
| MsG0080048321.01.T01 | MTR_4g079770 | 79.747 | 316 | 30 | 5 | 1 | 283 | 1 | 315 | 0.0 | 502 |
| MsG0080048321.01.T01 | MTR_4g107930 | 75.873 | 315 | 44 | 3 | 1 | 283 | 1 | 315 | 2.82e-174 | 485 |
| MsG0080048321.01.T01 | MTR_4g079470 | 79.661 | 295 | 27 | 4 | 21 | 283 | 22 | 315 | 1.18e-168 | 471 |
| MsG0080048321.01.T01 | MTR_4g079800 | 78.983 | 295 | 29 | 4 | 21 | 283 | 22 | 315 | 2.84e-168 | 470 |
| MsG0080048321.01.T01 | MTR_2g075830 | 67.197 | 314 | 69 | 4 | 1 | 283 | 1 | 311 | 1.05e-143 | 407 |
| MsG0080048321.01.T01 | MTR_4g080730 | 57.413 | 317 | 94 | 7 | 1 | 283 | 1 | 310 | 3.51e-118 | 343 |
| MsG0080048321.01.T01 | MTR_4g080700 | 59.727 | 293 | 77 | 7 | 25 | 283 | 25 | 310 | 1.01e-116 | 339 |
| MsG0080048321.01.T01 | MTR_4g080360 | 47.619 | 315 | 127 | 7 | 2 | 283 | 3 | 312 | 2.98e-97 | 290 |
| MsG0080048321.01.T01 | MTR_2g083930 | 47.742 | 310 | 116 | 7 | 12 | 283 | 13 | 314 | 3.38e-95 | 284 |
| MsG0080048321.01.T01 | MTR_4g104370 | 49.206 | 315 | 116 | 11 | 7 | 283 | 6 | 314 | 1.09e-89 | 271 |
| MsG0080048321.01.T01 | MTR_3g075390 | 47.284 | 313 | 118 | 9 | 14 | 283 | 7 | 315 | 1.31e-88 | 268 |
| MsG0080048321.01.T01 | MTR_7g078600 | 52.190 | 274 | 96 | 5 | 34 | 279 | 37 | 303 | 1.78e-88 | 267 |
| MsG0080048321.01.T01 | MTR_7g079230 | 45.928 | 307 | 132 | 5 | 1 | 279 | 1 | 301 | 5.53e-86 | 261 |
| MsG0080048321.01.T01 | MTR_3g116080 | 48.243 | 313 | 114 | 11 | 11 | 283 | 14 | 318 | 4.00e-85 | 259 |
| MsG0080048321.01.T01 | MTR_4g079440 | 47.872 | 282 | 110 | 5 | 33 | 283 | 40 | 315 | 1.01e-83 | 255 |
| MsG0080048321.01.T01 | MTR_1g107550 | 47.569 | 288 | 110 | 7 | 32 | 283 | 43 | 325 | 7.86e-83 | 257 |
| MsG0080048321.01.T01 | MTR_4g094918 | 41.534 | 313 | 137 | 7 | 10 | 283 | 6 | 311 | 4.43e-80 | 246 |
| MsG0080048321.01.T01 | MTR_2g090890 | 43.464 | 306 | 129 | 7 | 10 | 279 | 5 | 302 | 1.02e-79 | 244 |
| MsG0080048321.01.T01 | MTR_3g102220 | 44.631 | 298 | 117 | 8 | 27 | 283 | 41 | 331 | 1.18e-79 | 249 |
| MsG0080048321.01.T01 | MTR_1g018840 | 43.963 | 323 | 122 | 9 | 8 | 283 | 21 | 331 | 2.57e-78 | 245 |
| MsG0080048321.01.T01 | MTR_1g018840 | 42.012 | 338 | 134 | 9 | 1 | 283 | 1 | 331 | 4.42e-78 | 244 |
| MsG0080048321.01.T01 | MTR_2g015850 | 47.710 | 262 | 99 | 8 | 55 | 283 | 78 | 334 | 2.96e-73 | 229 |
| MsG0080048321.01.T01 | MTR_3g112430 | 45.035 | 282 | 111 | 7 | 40 | 283 | 37 | 312 | 3.68e-73 | 231 |
| MsG0080048321.01.T01 | MTR_2g016180 | 40.317 | 315 | 140 | 10 | 11 | 283 | 18 | 326 | 1.28e-63 | 207 |
| MsG0080048321.01.T01 | MTR_2g015840 | 41.786 | 280 | 122 | 7 | 7 | 283 | 1 | 242 | 1.03e-62 | 199 |
| MsG0080048321.01.T01 | MTR_2g016270 | 40.385 | 312 | 129 | 10 | 11 | 283 | 18 | 311 | 8.55e-62 | 202 |
| MsG0080048321.01.T01 | MTR_2g067900 | 43.750 | 288 | 114 | 12 | 38 | 283 | 15 | 296 | 5.08e-61 | 196 |
| MsG0080048321.01.T01 | MTR_7g078630 | 42.958 | 284 | 96 | 7 | 28 | 273 | 695 | 950 | 1.72e-59 | 204 |
| MsG0080048321.01.T01 | MTR_2g064475 | 38.141 | 312 | 113 | 14 | 38 | 279 | 15 | 316 | 9.42e-56 | 184 |
| MsG0080048321.01.T01 | MTR_2g066980 | 36.538 | 312 | 139 | 13 | 7 | 283 | 1 | 288 | 1.47e-55 | 182 |
| MsG0080048321.01.T01 | MTR_2g067560 | 42.373 | 295 | 112 | 14 | 38 | 283 | 15 | 300 | 5.39e-55 | 191 |
| MsG0080048321.01.T01 | MTR_4g065077 | 37.413 | 286 | 141 | 8 | 32 | 283 | 44 | 325 | 7.76e-53 | 176 |
| MsG0080048321.01.T01 | MTR_2g015920 | 38.488 | 291 | 131 | 10 | 33 | 283 | 47 | 329 | 1.53e-52 | 176 |
| MsG0080048321.01.T01 | MTR_4g125300 | 40.727 | 275 | 119 | 10 | 45 | 283 | 57 | 323 | 5.47e-51 | 171 |
| MsG0080048321.01.T01 | MTR_4g071060 | 34.604 | 341 | 145 | 12 | 9 | 283 | 3 | 331 | 1.64e-49 | 167 |
| MsG0080048321.01.T01 | MTR_2g015930 | 34.132 | 334 | 162 | 12 | 1 | 283 | 3 | 329 | 4.20e-48 | 164 |
| MsG0080048321.01.T01 | MTR_8g086470 | 35.563 | 284 | 133 | 8 | 32 | 283 | 60 | 325 | 2.17e-46 | 159 |
| MsG0080048321.01.T01 | MTR_1g023810 | 44.737 | 190 | 70 | 6 | 126 | 283 | 49 | 235 | 1.21e-44 | 152 |
| MsG0080048321.01.T01 | MTR_1g023210 | 34.177 | 316 | 140 | 12 | 23 | 283 | 33 | 335 | 2.96e-43 | 151 |
| MsG0080048321.01.T01 | MTR_1g094135 | 31.692 | 325 | 172 | 8 | 7 | 283 | 19 | 341 | 2.64e-40 | 144 |
| MsG0080048321.01.T01 | MTR_4g073660 | 35.968 | 253 | 139 | 9 | 46 | 283 | 54 | 298 | 7.76e-37 | 134 |
| MsG0080048321.01.T01 | MTR_4g072790 | 37.200 | 250 | 136 | 10 | 46 | 283 | 55 | 295 | 1.05e-36 | 133 |
| MsG0080048321.01.T01 | MTR_4g072340 | 36.614 | 254 | 136 | 10 | 46 | 283 | 55 | 299 | 1.05e-36 | 133 |
| MsG0080048321.01.T01 | MTR_1g025450 | 44.586 | 157 | 68 | 6 | 126 | 266 | 55 | 208 | 9.14e-34 | 129 |
| MsG0080048321.01.T01 | MTR_7g078310 | 42.553 | 141 | 72 | 3 | 82 | 220 | 115 | 248 | 1.00e-27 | 108 |
| MsG0080048321.01.T01 | MTR_7g078580 | 66.129 | 62 | 20 | 1 | 218 | 279 | 7 | 67 | 4.02e-21 | 87.0 |
| MsG0080048321.01.T01 | MTR_7g079130 | 64.516 | 62 | 21 | 1 | 223 | 283 | 7 | 68 | 9.84e-20 | 82.4 |
| MsG0080048321.01.T01 | MTR_2g047960 | 49.412 | 85 | 42 | 1 | 135 | 219 | 7 | 90 | 1.04e-18 | 79.7 |
| MsG0080048321.01.T01 | MTR_7g079260 | 73.171 | 41 | 11 | 0 | 239 | 279 | 7 | 47 | 1.61e-15 | 71.2 |
| MsG0080048321.01.T01 | MTR_1g042480 | 36.170 | 94 | 57 | 2 | 9 | 101 | 7 | 98 | 6.69e-12 | 62.0 |
| MsG0080048321.01.T01 | MTR_1g042360 | 36.170 | 94 | 57 | 2 | 9 | 101 | 7 | 98 | 6.69e-12 | 62.0 |
| MsG0080048321.01.T01 | MTR_2g016380 | 50.000 | 70 | 30 | 3 | 218 | 283 | 38 | 106 | 1.28e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048321.01.T01 | AT5G45890 | 50.949 | 316 | 114 | 8 | 7 | 283 | 3 | 316 | 3.62e-99 | 295 |
| MsG0080048321.01.T01 | AT5G50260 | 48.562 | 313 | 114 | 8 | 14 | 283 | 5 | 313 | 3.07e-92 | 277 |
| MsG0080048321.01.T01 | AT2G27420 | 45.455 | 319 | 124 | 8 | 10 | 283 | 5 | 318 | 3.50e-89 | 269 |
| MsG0080048321.01.T01 | AT2G34080 | 44.194 | 310 | 135 | 6 | 10 | 283 | 8 | 315 | 3.72e-88 | 266 |
| MsG0080048321.01.T01 | AT3G49340 | 45.806 | 310 | 116 | 8 | 20 | 283 | 8 | 311 | 4.17e-88 | 266 |
| MsG0080048321.01.T01 | AT3G48340 | 49.051 | 316 | 112 | 10 | 12 | 283 | 4 | 314 | 8.07e-88 | 266 |
| MsG0080048321.01.T01 | AT4G35350 | 45.912 | 318 | 126 | 8 | 7 | 285 | 14 | 324 | 3.85e-85 | 259 |
| MsG0080048321.01.T01 | AT3G48350 | 49.650 | 286 | 105 | 7 | 32 | 282 | 31 | 312 | 1.24e-84 | 258 |
| MsG0080048321.01.T01 | AT3G19390 | 45.364 | 302 | 130 | 5 | 14 | 283 | 18 | 316 | 2.58e-84 | 260 |
| MsG0080048321.01.T01 | AT1G29090 | 43.425 | 327 | 130 | 10 | 4 | 283 | 7 | 325 | 6.05e-81 | 248 |
| MsG0080048321.01.T01 | AT4G36880 | 44.932 | 296 | 116 | 6 | 32 | 285 | 42 | 332 | 2.33e-80 | 248 |
| MsG0080048321.01.T01 | AT5G43060 | 46.980 | 298 | 113 | 9 | 24 | 283 | 34 | 324 | 8.48e-80 | 249 |
| MsG0080048321.01.T01 | AT1G20850 | 46.454 | 282 | 115 | 5 | 33 | 282 | 45 | 322 | 1.25e-79 | 245 |
| MsG0080048321.01.T01 | AT3G19400 | 44.689 | 273 | 117 | 3 | 39 | 279 | 44 | 314 | 1.32e-78 | 243 |
| MsG0080048321.01.T01 | AT1G47128 | 45.907 | 281 | 109 | 9 | 39 | 283 | 50 | 323 | 3.81e-77 | 242 |
| MsG0080048321.01.T01 | AT1G06260 | 43.631 | 314 | 129 | 8 | 9 | 283 | 9 | 313 | 6.08e-76 | 235 |
| MsG0080048321.01.T01 | AT1G09850 | 45.390 | 282 | 120 | 5 | 33 | 283 | 26 | 304 | 3.26e-74 | 234 |
| MsG0080048321.01.T01 | AT1G29080 | 40.728 | 302 | 130 | 8 | 24 | 283 | 22 | 316 | 5.57e-72 | 225 |
| MsG0080048321.01.T01 | AT4G11320 | 39.721 | 287 | 117 | 7 | 40 | 283 | 57 | 330 | 7.43e-69 | 218 |
| MsG0080048321.01.T01 | AT4G11320 | 39.721 | 287 | 117 | 7 | 40 | 283 | 57 | 330 | 7.43e-69 | 218 |
| MsG0080048321.01.T01 | AT4G23520 | 39.130 | 322 | 140 | 10 | 10 | 283 | 7 | 320 | 2.10e-68 | 216 |
| MsG0080048321.01.T01 | AT4G35350 | 43.571 | 280 | 112 | 8 | 7 | 247 | 14 | 286 | 2.78e-68 | 214 |
| MsG0080048321.01.T01 | AT4G11310 | 40.925 | 281 | 122 | 7 | 40 | 283 | 50 | 323 | 7.23e-68 | 215 |
| MsG0080048321.01.T01 | AT3G48350 | 47.410 | 251 | 93 | 7 | 32 | 247 | 31 | 277 | 9.53e-66 | 207 |
| MsG0080048321.01.T01 | AT3G43960 | 39.203 | 301 | 146 | 5 | 16 | 282 | 18 | 315 | 1.02e-63 | 205 |
| MsG0080048321.01.T01 | AT3G19400 | 42.324 | 241 | 106 | 2 | 39 | 247 | 44 | 283 | 1.15e-62 | 199 |
| MsG0080048321.01.T01 | AT1G29110 | 38.567 | 293 | 126 | 9 | 29 | 283 | 28 | 304 | 2.89e-58 | 189 |
| MsG0080048321.01.T01 | AT5G60360 | 41.971 | 274 | 117 | 10 | 45 | 283 | 65 | 331 | 3.31e-53 | 177 |
| MsG0080048321.01.T01 | AT5G60360 | 41.971 | 274 | 117 | 10 | 45 | 283 | 65 | 331 | 3.35e-53 | 177 |
| MsG0080048321.01.T01 | AT5G60360 | 41.971 | 274 | 117 | 10 | 45 | 283 | 65 | 331 | 4.87e-53 | 177 |
| MsG0080048321.01.T01 | AT3G45310 | 40.876 | 274 | 120 | 10 | 45 | 283 | 65 | 331 | 7.65e-51 | 171 |
| MsG0080048321.01.T01 | AT3G45310 | 40.876 | 274 | 120 | 10 | 45 | 283 | 65 | 331 | 7.67e-51 | 171 |
| MsG0080048321.01.T01 | AT4G39090 | 37.631 | 287 | 121 | 10 | 46 | 283 | 58 | 335 | 4.28e-48 | 164 |
| MsG0080048321.01.T01 | AT2G21430 | 36.491 | 285 | 127 | 9 | 46 | 283 | 55 | 332 | 5.52e-47 | 161 |
| MsG0080048321.01.T01 | AT4G16190 | 36.364 | 286 | 127 | 9 | 46 | 283 | 62 | 340 | 3.31e-45 | 157 |
| MsG0080048321.01.T01 | AT3G54940 | 34.130 | 293 | 141 | 8 | 38 | 283 | 50 | 337 | 3.23e-42 | 149 |
| MsG0080048321.01.T01 | AT3G54940 | 39.320 | 206 | 109 | 4 | 38 | 232 | 50 | 250 | 1.34e-40 | 142 |
| MsG0080048321.01.T01 | AT2G22160 | 40.777 | 103 | 56 | 2 | 43 | 145 | 1 | 98 | 3.40e-15 | 70.5 |
Find 75 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGCATTGCTTCTATGTT+TGG | 0.245347 | contig271end:-263007 | MsG0080048321.01.T01:CDS |
| TGATAAAGAGATCAACTTTC+CGG | 0.269436 | contig271end:+262443 | None:intergenic |
| AACACCTTGATAAGGGTAAT+TGG | 0.282835 | contig271end:+262308 | None:intergenic |
| TTGGCATTGCTTCTATGTTT+GGG | 0.292590 | contig271end:-263006 | MsG0080048321.01.T01:CDS |
| TCACTATGGCAAAGTTTATA+AGG | 0.293530 | contig271end:-262908 | MsG0080048321.01.T01:CDS |
| AATATGATATAAGTGATTAT+TGG | 0.319024 | contig271end:+263032 | None:intergenic |
| AGGTTGTGAGGGTGGTCTTA+TGG | 0.350865 | contig271end:-262379 | MsG0080048321.01.T01:CDS |
| AAATTCATCATTCAAAATCA+TGG | 0.364764 | contig271end:-262345 | MsG0080048321.01.T01:CDS |
| TATTGGTTGGTTAAGAACTC+TGG | 0.401763 | contig271end:-262039 | MsG0080048321.01.T01:CDS |
| TCACTTATATCATATTTCAT+TGG | 0.405472 | contig271end:-263025 | MsG0080048321.01.T01:CDS |
| CAGCAATGGATGGGTCACTA+TGG | 0.420749 | contig271end:-262922 | MsG0080048321.01.T01:CDS |
| CATAGTGACCCATCCATTGC+TGG | 0.428097 | contig271end:+262923 | None:intergenic |
| GCATCAATGGCTACAGAAAT+CGG | 0.431804 | contig271end:+262177 | None:intergenic |
| AGTCAATTTATGAATTCCTT+CGG | 0.437717 | contig271end:+262467 | None:intergenic |
| CATTGTCCTTGGTTCTTCAC+AGG | 0.444720 | contig271end:+262622 | None:intergenic |
| AACTCTGGGGAACCTTGGAT+TGG | 0.454674 | contig271end:-262024 | MsG0080048321.01.T01:CDS |
| TTCCGTCAACACCTTGATAA+GGG | 0.456935 | contig271end:+262301 | None:intergenic |
| GTCAGATCCACTAGCATCAA+TGG | 0.459170 | contig271end:+262164 | None:intergenic |
| AACACATACCACATTGTCCT+TGG | 0.464008 | contig271end:+262611 | None:intergenic |
| GTTCCGTCAACACCTTGATA+AGG | 0.464383 | contig271end:+262300 | None:intergenic |
| TTGATAAGGGTAATTGGCTT+CGG | 0.466155 | contig271end:+262314 | None:intergenic |
| TTTATAAGGATTCTCAAGAA+AGG | 0.473803 | contig271end:-262894 | MsG0080048321.01.T01:CDS |
| TCTCATACATGGAGTCATCT+TGG | 0.474251 | contig271end:+262950 | None:intergenic |
| CAACTCAGTTCCGCATGAAC+CGG | 0.479102 | contig271end:+262113 | None:intergenic |
| TTGCTGGTGCCTCTCATACA+TGG | 0.480269 | contig271end:+262939 | None:intergenic |
| ATTGGTTGGTTAAGAACTCT+GGG | 0.482511 | contig271end:-262038 | MsG0080048321.01.T01:CDS |
| TTCCTCCAATCCACTGAGGA+TGG | 0.487802 | contig271end:+262661 | None:intergenic |
| TTATAAGGATTCTCAAGAAA+GGG | 0.488285 | contig271end:-262893 | MsG0080048321.01.T01:CDS |
| CAATTCCATCCTCAGTGGAT+TGG | 0.490367 | contig271end:-262666 | MsG0080048321.01.T01:CDS |
| ATCATCTAGAAACAAATTCA+AGG | 0.491052 | contig271end:-262746 | MsG0080048321.01.T01:CDS |
| TTAAGAACTCTGGGGAACCT+TGG | 0.498847 | contig271end:-262029 | MsG0080048321.01.T01:CDS |
| TTCCATCCTCAGTGGATTGG+AGG | 0.507592 | contig271end:-262663 | MsG0080048321.01.T01:CDS |
| ACTCTGGGGAACCTTGGATT+GGG | 0.508314 | contig271end:-262023 | MsG0080048321.01.T01:CDS |
| GGTGTTACCGCTGTGGGTTA+CGG | 0.510469 | contig271end:-262084 | MsG0080048321.01.T01:CDS |
| TACATTGAAGCTTTCAACAA+AGG | 0.517957 | contig271end:-262835 | MsG0080048321.01.T01:CDS |
| CGGTTCATGCGGAACTGAGT+TGG | 0.529631 | contig271end:-262112 | MsG0080048321.01.T01:CDS |
| TCAGTGGATTGGAGGAAGAA+AGG | 0.529739 | contig271end:-262655 | MsG0080048321.01.T01:CDS |
| TCTGTAGCCATTGATGCTAG+TGG | 0.531179 | contig271end:-262171 | MsG0080048321.01.T01:CDS |
| GACTTTCAATTCTACAAAAG+CGG | 0.531410 | contig271end:-262144 | MsG0080048321.01.T01:CDS |
| TGAGAGGCACCAGCAATGGA+TGG | 0.535269 | contig271end:-262932 | MsG0080048321.01.T01:CDS |
| GTGACGACGGAACTAAGTAT+TGG | 0.539182 | contig271end:-262056 | MsG0080048321.01.T01:CDS |
| GGAATTCATAAATTGACTAC+CGG | 0.539888 | contig271end:-262462 | MsG0080048321.01.T01:CDS |
| GTGGACCAAGGTTGTGAGGG+TGG | 0.540762 | contig271end:-262387 | MsG0080048321.01.T01:CDS |
| CGACGGAACTAAGTATTGGT+TGG | 0.550478 | contig271end:-262052 | MsG0080048321.01.T01:CDS |
| TGTATGAGAGGCACCAGCAA+TGG | 0.552809 | contig271end:-262936 | MsG0080048321.01.T01:CDS |
| TCATCTAGAAACAAATTCAA+GGG | 0.562761 | contig271end:-262745 | MsG0080048321.01.T01:CDS |
| TCTGCTGTTGCAGCAACCGA+AGG | 0.563495 | contig271end:-262483 | MsG0080048321.01.T01:CDS |
| ATCCTTCTTCACCCAATCCA+AGG | 0.564469 | contig271end:+262012 | None:intergenic |
| TAAGTGATTATTGGCAGCCA+TGG | 0.564524 | contig271end:+263041 | None:intergenic |
| TGCGGAACTGAGTTGGATCA+TGG | 0.566419 | contig271end:-262105 | MsG0080048321.01.T01:CDS |
| GAAGCCAATTACCCTTATCA+AGG | 0.574336 | contig271end:-262312 | MsG0080048321.01.T01:intron |
| TAAGACCACCCTCACAACCT+TGG | 0.579957 | contig271end:+262382 | None:intergenic |
| GAGAGGCACCAGCAATGGAT+GGG | 0.587800 | contig271end:-262931 | MsG0080048321.01.T01:CDS |
| ATGTGAACTCGTCGTTGGTG+AGG | 0.594073 | contig271end:+262767 | None:intergenic |
| GATCATGGTGTTACCGCTGT+GGG | 0.595355 | contig271end:-262090 | MsG0080048321.01.T01:CDS |
| GTTTATCACACTAGCTACCA+TGG | 0.599656 | contig271end:-263058 | None:intergenic |
| GGATCATGGTGTTACCGCTG+TGG | 0.606355 | contig271end:-262091 | MsG0080048321.01.T01:CDS |
| GAACTCGTCGTTGGTGAGGT+CGG | 0.607945 | contig271end:+262771 | None:intergenic |
| GGTGTGGACCAAGGTTGTGA+GGG | 0.612032 | contig271end:-262390 | MsG0080048321.01.T01:CDS |
| CTCGTTGATTGTGACACGAA+AGG | 0.633361 | contig271end:-262411 | MsG0080048321.01.T01:CDS |
| AGATGATGTGAACTCGTCGT+TGG | 0.635437 | contig271end:+262762 | None:intergenic |
| AGGTGTGGACCAAGGTTGTG+AGG | 0.640947 | contig271end:-262391 | MsG0080048321.01.T01:CDS |
| CATCTAGAAACAAATTCAAG+GGG | 0.642978 | contig271end:-262744 | MsG0080048321.01.T01:CDS |
| AAGATGACTCCATGTATGAG+AGG | 0.643922 | contig271end:-262948 | MsG0080048321.01.T01:CDS |
| ATATGTGCTCTTCAATCACA+AGG | 0.656906 | contig271end:-262720 | MsG0080048321.01.T01:CDS |
| AATAACAAGCTATACACACT+AGG | 0.659674 | contig271end:-262808 | MsG0080048321.01.T01:CDS |
| TTTCTTCCTCCAATCCACTG+AGG | 0.673228 | contig271end:+262657 | None:intergenic |
| GTTACACCTGTGAAGAACCA+AGG | 0.683178 | contig271end:-262628 | MsG0080048321.01.T01:CDS |
| GTGAAGAACCAAGGACAATG+TGG | 0.691000 | contig271end:-262619 | MsG0080048321.01.T01:intron |
| GGTTACGGTGTTAGTGACGA+CGG | 0.694509 | contig271end:-262069 | MsG0080048321.01.T01:CDS |
| GACACGAAAGGTGTGGACCA+AGG | 0.716503 | contig271end:-262399 | MsG0080048321.01.T01:CDS |
| TGATTGTGACACGAAAGGTG+TGG | 0.726786 | contig271end:-262406 | MsG0080048321.01.T01:CDS |
| ACTAACACCGTAACCCACAG+CGG | 0.728918 | contig271end:+262077 | None:intergenic |
| AAGTGCAATTCCATCCTCAG+TGG | 0.731023 | contig271end:-262671 | MsG0080048321.01.T01:CDS |
| TTGGTTGGTTAAGAACTCTG+GGG | 0.750152 | contig271end:-262037 | MsG0080048321.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTAATTTTCATTTTGT+AGG | - | contig271end:262548-262567 | MsG0080048321.01.T01:intron | 10.0% |
| !! | AATATGATATAAGTGATTAT+TGG | + | contig271end:262041-262060 | None:intergenic | 15.0% |
| !!! | TGATAACTAATTTTTTTATC+AGG | + | contig271end:262515-262534 | None:intergenic | 15.0% |
| !! | AAATTCATCATTCAAAATCA+TGG | - | contig271end:262725-262744 | MsG0080048321.01.T01:CDS | 20.0% |
| !! | TCACTTATATCATATTTCAT+TGG | - | contig271end:262045-262064 | MsG0080048321.01.T01:CDS | 20.0% |
| ! | AGTCAATTTATGAATTCCTT+CGG | + | contig271end:262606-262625 | None:intergenic | 25.0% |
| ! | ATCATCTAGAAACAAATTCA+AGG | - | contig271end:262324-262343 | MsG0080048321.01.T01:CDS | 25.0% |
| ! | TCATCTAGAAACAAATTCAA+GGG | - | contig271end:262325-262344 | MsG0080048321.01.T01:CDS | 25.0% |
| ! | TTATAAGGATTCTCAAGAAA+GGG | - | contig271end:262177-262196 | MsG0080048321.01.T01:CDS | 25.0% |
| ! | TTTATAAGGATTCTCAAGAA+AGG | - | contig271end:262176-262195 | MsG0080048321.01.T01:CDS | 25.0% |
| AATAACAAGCTATACACACT+AGG | - | contig271end:262262-262281 | MsG0080048321.01.T01:intron | 30.0% | |
| CATCTAGAAACAAATTCAAG+GGG | - | contig271end:262326-262345 | MsG0080048321.01.T01:CDS | 30.0% | |
| GACTTTCAATTCTACAAAAG+CGG | - | contig271end:262926-262945 | MsG0080048321.01.T01:CDS | 30.0% | |
| GGAATTCATAAATTGACTAC+CGG | - | contig271end:262608-262627 | MsG0080048321.01.T01:intron | 30.0% | |
| TACATTGAAGCTTTCAACAA+AGG | - | contig271end:262235-262254 | MsG0080048321.01.T01:intron | 30.0% | |
| TCACTATGGCAAAGTTTATA+AGG | - | contig271end:262162-262181 | MsG0080048321.01.T01:CDS | 30.0% | |
| TGATAAAGAGATCAACTTTC+CGG | + | contig271end:262630-262649 | None:intergenic | 30.0% | |
| ! | TCATTTTGTAGGATGTTGTT+GGG | - | contig271end:262559-262578 | MsG0080048321.01.T01:intron | 30.0% |
| ! | TTCATTTTGTAGGATGTTGT+TGG | - | contig271end:262558-262577 | MsG0080048321.01.T01:intron | 30.0% |
| AACACCTTGATAAGGGTAAT+TGG | + | contig271end:262765-262784 | None:intergenic | 35.0% | |
| ATATGTGCTCTTCAATCACA+AGG | - | contig271end:262350-262369 | MsG0080048321.01.T01:CDS | 35.0% | |
| ATTGGCATTGCTTCTATGTT+TGG | - | contig271end:262063-262082 | MsG0080048321.01.T01:CDS | 35.0% | |
| TTGGCATTGCTTCTATGTTT+GGG | - | contig271end:262064-262083 | MsG0080048321.01.T01:CDS | 35.0% | |
| ! | ATTGGTTGGTTAAGAACTCT+GGG | - | contig271end:263032-263051 | MsG0080048321.01.T01:CDS | 35.0% |
| ! | TATTGGTTGGTTAAGAACTC+TGG | - | contig271end:263031-263050 | MsG0080048321.01.T01:CDS | 35.0% |
| ! | TTGATAAGGGTAATTGGCTT+CGG | + | contig271end:262759-262778 | None:intergenic | 35.0% |
| AACACATACCACATTGTCCT+TGG | + | contig271end:262462-262481 | None:intergenic | 40.0% | |
| AAGATGACTCCATGTATGAG+AGG | - | contig271end:262122-262141 | MsG0080048321.01.T01:CDS | 40.0% | |
| AGTAATGGTAACTGCGTTGA+TGG | + | contig271end:262816-262835 | None:intergenic | 40.0% | |
| ATCAACGCAGTTACCATTAC+TGG | - | contig271end:262815-262834 | MsG0080048321.01.T01:CDS | 40.0% | |
| GAAGCCAATTACCCTTATCA+AGG | - | contig271end:262758-262777 | MsG0080048321.01.T01:CDS | 40.0% | |
| GACATCTTCATACCCAGTAA+TGG | + | contig271end:262831-262850 | None:intergenic | 40.0% | |
| GCATCAATGGCTACAGAAAT+CGG | + | contig271end:262896-262915 | None:intergenic | 40.0% | |
| GGAACATGCAATGCAAACAA+AGG | - | contig271end:262788-262807 | MsG0080048321.01.T01:CDS | 40.0% | |
| TAAGTGATTATTGGCAGCCA+TGG | + | contig271end:262032-262051 | None:intergenic | 40.0% | |
| TCAACGCAGTTACCATTACT+GGG | - | contig271end:262816-262835 | MsG0080048321.01.T01:CDS | 40.0% | |
| TCTCATACATGGAGTCATCT+TGG | + | contig271end:262123-262142 | None:intergenic | 40.0% | |
| TTCCGTCAACACCTTGATAA+GGG | + | contig271end:262772-262791 | None:intergenic | 40.0% | |
| ! | TACAAAAGCGGTGTTTTCAC+CGG | - | contig271end:262938-262957 | MsG0080048321.01.T01:CDS | 40.0% |
| ! | TTGGTTGGTTAAGAACTCTG+GGG | - | contig271end:263033-263052 | MsG0080048321.01.T01:CDS | 40.0% |
| !! | TACCCTTATCAAGGTGTTGA+CGG | - | contig271end:262767-262786 | MsG0080048321.01.T01:CDS | 40.0% |
| !! | TTTTTGTAGTGCTGCTCATG+TGG | + | contig271end:262858-262877 | None:intergenic | 40.0% |
| AAGTGCAATTCCATCCTCAG+TGG | - | contig271end:262399-262418 | MsG0080048321.01.T01:CDS | 45.0% | |
| AGATGATGTGAACTCGTCGT+TGG | + | contig271end:262311-262330 | None:intergenic | 45.0% | |
| AGCAGCACTACAAAAAGCAG+TGG | - | contig271end:262862-262881 | MsG0080048321.01.T01:CDS | 45.0% | |
| CAATTCCATCCTCAGTGGAT+TGG | - | contig271end:262404-262423 | MsG0080048321.01.T01:CDS | 45.0% | |
| GTCAGATCCACTAGCATCAA+TGG | + | contig271end:262909-262928 | None:intergenic | 45.0% | |
| GTGAAGAACCAAGGACAATG+TGG | - | contig271end:262451-262470 | MsG0080048321.01.T01:CDS | 45.0% | |
| GTGACGACGGAACTAAGTAT+TGG | - | contig271end:263014-263033 | MsG0080048321.01.T01:CDS | 45.0% | |
| GTTACACCTGTGAAGAACCA+AGG | - | contig271end:262442-262461 | MsG0080048321.01.T01:CDS | 45.0% | |
| GTTCCGTCAACACCTTGATA+AGG | + | contig271end:262773-262792 | None:intergenic | 45.0% | |
| TACTGGGTATGAAGATGTCC+CGG | - | contig271end:262832-262851 | MsG0080048321.01.T01:CDS | 45.0% | |
| TCTGTAGCCATTGATGCTAG+TGG | - | contig271end:262899-262918 | MsG0080048321.01.T01:CDS | 45.0% | |
| TGATTGTGACACGAAAGGTG+TGG | - | contig271end:262664-262683 | MsG0080048321.01.T01:CDS | 45.0% | |
| TTTCTTCCTCCAATCCACTG+AGG | + | contig271end:262416-262435 | None:intergenic | 45.0% | |
| ! | CATTGTCCTTGGTTCTTCAC+AGG | + | contig271end:262451-262470 | None:intergenic | 45.0% |
| ! | CTCGTTGATTGTGACACGAA+AGG | - | contig271end:262659-262678 | MsG0080048321.01.T01:CDS | 45.0% |
| ! | TTAAGAACTCTGGGGAACCT+TGG | - | contig271end:263041-263060 | MsG0080048321.01.T01:CDS | 45.0% |
| !! | CGACGGAACTAAGTATTGGT+TGG | - | contig271end:263018-263037 | MsG0080048321.01.T01:CDS | 45.0% |
| !! | TCAGTGGATTGGAGGAAGAA+AGG | - | contig271end:262415-262434 | MsG0080048321.01.T01:CDS | 45.0% |
| ACTAACACCGTAACCCACAG+CGG | + | contig271end:262996-263015 | None:intergenic | 50.0% | |
| ATGTGAACTCGTCGTTGGTG+AGG | + | contig271end:262306-262325 | None:intergenic | 50.0% | |
| CAACTCAGTTCCGCATGAAC+CGG | + | contig271end:262960-262979 | None:intergenic | 50.0% | |
| CAGCAATGGATGGGTCACTA+TGG | - | contig271end:262148-262167 | MsG0080048321.01.T01:CDS | 50.0% | |
| CATAGTGACCCATCCATTGC+TGG | + | contig271end:262150-262169 | None:intergenic | 50.0% | |
| TAAGACCACCCTCACAACCT+TGG | + | contig271end:262691-262710 | None:intergenic | 50.0% | |
| TTCCTCCAATCCACTGAGGA+TGG | + | contig271end:262412-262431 | None:intergenic | 50.0% | |
| TTGCTGGTGCCTCTCATACA+TGG | + | contig271end:262134-262153 | None:intergenic | 50.0% | |
| ! | AACTCTGGGGAACCTTGGAT+TGG | - | contig271end:263046-263065 | MsG0080048321.01.T01:exon | 50.0% |
| ! | ACTCTGGGGAACCTTGGATT+GGG | - | contig271end:263047-263066 | MsG0080048321.01.T01:exon | 50.0% |
| ! | AGGTTGTGAGGGTGGTCTTA+TGG | - | contig271end:262691-262710 | MsG0080048321.01.T01:CDS | 50.0% |
| ! | GGTTACGGTGTTAGTGACGA+CGG | - | contig271end:263001-263020 | MsG0080048321.01.T01:CDS | 50.0% |
| ! | TGCGGAACTGAGTTGGATCA+TGG | - | contig271end:262965-262984 | MsG0080048321.01.T01:CDS | 50.0% |
| !! | GATCATGGTGTTACCGCTGT+GGG | - | contig271end:262980-262999 | MsG0080048321.01.T01:CDS | 50.0% |
| !! | TGTATGAGAGGCACCAGCAA+TGG | - | contig271end:262134-262153 | MsG0080048321.01.T01:CDS | 50.0% |
| !! | TTCCATCCTCAGTGGATTGG+AGG | - | contig271end:262407-262426 | MsG0080048321.01.T01:CDS | 50.0% |
| !!! | GGTGTTTTCACCGGTTCATG+CGG | - | contig271end:262947-262966 | MsG0080048321.01.T01:CDS | 50.0% |
| AGGTGTGGACCAAGGTTGTG+AGG | - | contig271end:262679-262698 | MsG0080048321.01.T01:CDS | 55.0% | |
| CGGTTCATGCGGAACTGAGT+TGG | - | contig271end:262958-262977 | MsG0080048321.01.T01:CDS | 55.0% | |
| GGTGTGGACCAAGGTTGTGA+GGG | - | contig271end:262680-262699 | MsG0080048321.01.T01:CDS | 55.0% | |
| TCTGCTGTTGCAGCAACCGA+AGG | - | contig271end:262587-262606 | MsG0080048321.01.T01:intron | 55.0% | |
| ! | GAACTCGTCGTTGGTGAGGT+CGG | + | contig271end:262302-262321 | None:intergenic | 55.0% |
| ! | GACACGAAAGGTGTGGACCA+AGG | - | contig271end:262671-262690 | MsG0080048321.01.T01:CDS | 55.0% |
| ! | GGTGTTACCGCTGTGGGTTA+CGG | - | contig271end:262986-263005 | MsG0080048321.01.T01:CDS | 55.0% |
| ! | TGTAGTGCTGCTCATGTGGC+CGG | + | contig271end:262854-262873 | None:intergenic | 55.0% |
| !! | GAGAGGCACCAGCAATGGAT+GGG | - | contig271end:262139-262158 | MsG0080048321.01.T01:CDS | 55.0% |
| !! | GGATCATGGTGTTACCGCTG+TGG | - | contig271end:262979-262998 | MsG0080048321.01.T01:CDS | 55.0% |
| !! | TGAGAGGCACCAGCAATGGA+TGG | - | contig271end:262138-262157 | MsG0080048321.01.T01:CDS | 55.0% |
| GTGGACCAAGGTTGTGAGGG+TGG | - | contig271end:262683-262702 | MsG0080048321.01.T01:CDS | 60.0% | |
| ! | GTAGTGCTGCTCATGTGGCC+GGG | + | contig271end:262853-262872 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig271end | gene | 262020 | 263072 | 262020 | ID=MsG0080048321.01;Name=MsG0080048321.01 |
| contig271end | mRNA | 262020 | 263072 | 262020 | ID=MsG0080048321.01.T01;Parent=MsG0080048321.01;Name=MsG0080048321.01.T01;_AED=0.43;_eAED=0.43;_QI=11|0.5|0.33|1|1|1|3|0|285 |
| contig271end | exon | 262620 | 263072 | 262620 | ID=MsG0080048321.01.T01:exon:19227;Parent=MsG0080048321.01.T01 |
| contig271end | exon | 262313 | 262522 | 262313 | ID=MsG0080048321.01.T01:exon:19228;Parent=MsG0080048321.01.T01 |
| contig271end | exon | 262020 | 262225 | 262020 | ID=MsG0080048321.01.T01:exon:19229;Parent=MsG0080048321.01.T01 |
| contig271end | five_prime_UTR | 263062 | 263072 | 263062 | ID=MsG0080048321.01.T01:five_prime_utr;Parent=MsG0080048321.01.T01 |
| contig271end | CDS | 262620 | 263061 | 262620 | ID=MsG0080048321.01.T01:cds;Parent=MsG0080048321.01.T01 |
| contig271end | CDS | 262313 | 262522 | 262313 | ID=MsG0080048321.01.T01:cds;Parent=MsG0080048321.01.T01 |
| contig271end | CDS | 262020 | 262225 | 262020 | ID=MsG0080048321.01.T01:cds;Parent=MsG0080048321.01.T01 |
| Gene Sequence |
| Protein sequence |
