AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005131.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005131.01.T01 MTR_1g094135 97.113 381 11 0 1 381 1 381 0.0 781
MsG0180005131.01.T01 MTR_1g023210 52.151 372 168 4 1 370 1 364 1.77e-140 404
MsG0180005131.01.T01 MTR_4g071060 53.482 359 156 6 15 370 10 360 3.83e-140 403
MsG0180005131.01.T01 MTR_4g073660 43.288 365 162 7 8 370 6 327 5.13e-100 300
MsG0180005131.01.T01 MTR_4g072790 43.443 366 156 9 8 370 7 324 3.43e-99 298
MsG0180005131.01.T01 MTR_4g072340 43.380 355 157 6 17 370 17 328 1.89e-98 296
MsG0180005131.01.T01 MTR_3g116080 36.857 350 191 8 22 363 17 344 1.36e-62 204
MsG0180005131.01.T01 MTR_4g079440 34.626 361 205 10 15 367 8 345 1.65e-61 201
MsG0180005131.01.T01 MTR_4g107930 35.112 356 196 10 20 363 9 341 4.11e-59 195
MsG0180005131.01.T01 MTR_5g022560 34.734 357 198 10 19 363 7 340 8.45e-59 194
MsG0180005131.01.T01 MTR_4g047610 36.025 322 176 8 54 363 36 339 8.58e-59 194
MsG0180005131.01.T01 MTR_4g079470 36.499 337 177 11 35 358 24 336 1.42e-57 191
MsG0180005131.01.T01 MTR_4g079770 36.585 328 173 11 44 358 31 336 1.51e-57 191
MsG0180005131.01.T01 MTR_4g080730 35.207 338 176 9 37 358 21 331 3.03e-57 190
MsG0180005131.01.T01 MTR_4g094918 32.718 379 198 11 1 363 1 338 9.58e-57 189
MsG0180005131.01.T01 MTR_4g125300 37.812 320 172 9 56 368 51 350 2.44e-56 188
MsG0180005131.01.T01 MTR_2g016180 36.449 321 172 12 56 363 51 352 5.37e-56 191
MsG0180005131.01.T01 MTR_4g065077 33.422 374 209 11 2 362 6 352 5.48e-56 187
MsG0180005131.01.T01 MTR_4g080700 35.168 327 169 9 48 358 32 331 5.61e-56 187
MsG0180005131.01.T01 MTR_4g079800 34.535 333 183 11 44 363 31 341 2.39e-55 186
MsG0180005131.01.T01 MTR_1g107550 35.988 339 181 12 35 358 30 347 2.82e-55 189
MsG0180005131.01.T01 MTR_4g104370 34.320 338 192 10 34 358 15 335 3.07e-55 186
MsG0180005131.01.T01 MTR_2g090890 35.443 316 171 9 53 358 36 328 5.77e-55 184
MsG0180005131.01.T01 MTR_2g016270 35.826 321 159 12 56 363 51 337 1.33e-54 187
MsG0180005131.01.T01 MTR_1g018840 33.602 372 203 10 5 358 8 353 5.10e-54 185
MsG0180005131.01.T01 MTR_1g018840 33.602 372 203 10 5 358 8 353 5.90e-54 185
MsG0180005131.01.T01 MTR_8g086470 32.703 370 201 11 2 362 22 352 1.61e-53 181
MsG0180005131.01.T01 MTR_2g015850 38.768 276 143 10 100 367 107 364 1.22e-52 179
MsG0180005131.01.T01 MTR_3g075390 32.544 338 199 10 34 358 15 336 1.97e-52 178
MsG0180005131.01.T01 MTR_3g112430 34.769 325 181 11 52 363 32 338 3.04e-51 177
MsG0180005131.01.T01 MTR_3g102220 35.667 300 155 10 76 358 75 353 9.35e-51 177
MsG0180005131.01.T01 MTR_2g083930 34.036 332 181 10 54 367 33 344 1.52e-49 170
MsG0180005131.01.T01 MTR_2g075830 35.016 317 163 12 59 358 42 332 7.25e-49 168
MsG0180005131.01.T01 MTR_4g080360 34.891 321 173 12 55 360 36 335 1.09e-48 168
MsG0180005131.01.T01 MTR_2g015920 29.211 380 202 13 12 360 9 352 1.12e-46 164
MsG0180005131.01.T01 MTR_2g067900 33.735 332 183 12 50 363 10 322 8.63e-46 160
MsG0180005131.01.T01 MTR_7g078600 37.131 237 126 7 137 367 119 338 3.24e-44 156
MsG0180005131.01.T01 MTR_2g015930 30.163 368 213 12 12 359 8 351 9.99e-44 155
MsG0180005131.01.T01 MTR_7g079230 31.288 326 186 11 45 358 28 327 1.78e-43 154
MsG0180005131.01.T01 MTR_2g067560 33.429 350 185 16 50 373 10 337 2.07e-40 152
MsG0180005131.01.T01 MTR_8g087510 47.015 134 62 1 182 315 23 147 4.14e-40 140
MsG0180005131.01.T01 MTR_1g023810 39.286 224 114 8 138 357 46 251 5.29e-39 140
MsG0180005131.01.T01 MTR_2g066980 31.642 335 152 14 54 363 32 314 2.05e-33 127
MsG0180005131.01.T01 MTR_2g064475 28.962 366 192 13 50 372 10 350 2.98e-33 127
MsG0180005131.01.T01 MTR_7g078630 34.715 193 99 6 137 315 762 941 1.72e-24 105
MsG0180005131.01.T01 MTR_1g025450 39.655 116 58 3 136 248 50 156 2.69e-19 89.7
MsG0180005131.01.T01 MTR_2g015840 35.862 145 83 5 59 194 36 179 8.91e-17 80.1
MsG0180005131.01.T01 MTR_7g078310 40.741 108 55 2 137 242 148 248 2.19e-16 79.0
MsG0180005131.01.T01 MTR_7g111050 26.420 352 204 15 49 366 5 335 9.78e-16 78.2
MsG0180005131.01.T01 MTR_2g047960 43.478 92 44 1 145 236 2 85 3.63e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005131.01.T01 AT3G54940 70.118 338 93 2 35 364 23 360 0.0 511
MsG0180005131.01.T01 AT2G21430 56.495 331 138 4 34 363 29 354 2.83e-139 401
MsG0180005131.01.T01 AT4G39090 57.362 326 134 4 48 370 43 366 6.40e-138 398
MsG0180005131.01.T01 AT4G16190 56.418 335 137 6 43 372 42 372 2.94e-131 381
MsG0180005131.01.T01 AT3G54940 66.142 254 78 2 35 280 23 276 4.96e-123 357
MsG0180005131.01.T01 AT1G20850 38.750 320 164 8 56 363 51 350 1.91e-66 214
MsG0180005131.01.T01 AT4G35350 34.341 364 210 8 8 363 7 349 1.39e-62 204
MsG0180005131.01.T01 AT5G60360 38.109 349 178 10 34 368 34 358 3.20e-62 204
MsG0180005131.01.T01 AT5G60360 38.166 338 171 10 34 357 34 347 4.75e-60 198
MsG0180005131.01.T01 AT3G45310 35.979 378 204 14 7 368 3 358 9.75e-60 197
MsG0180005131.01.T01 AT5G60360 37.822 349 178 11 34 368 34 357 1.07e-59 197
MsG0180005131.01.T01 AT1G29090 38.924 316 158 12 59 358 50 346 1.52e-59 197
MsG0180005131.01.T01 AT5G45890 37.576 330 168 11 46 358 29 337 1.66e-59 196
MsG0180005131.01.T01 AT1G06260 36.471 340 181 11 38 363 21 339 7.04e-58 192
MsG0180005131.01.T01 AT3G45310 35.792 366 197 14 7 356 3 346 2.36e-57 191
MsG0180005131.01.T01 AT5G43060 33.424 368 201 13 5 358 8 345 1.04e-56 192
MsG0180005131.01.T01 AT3G19400 35.777 341 182 12 38 363 27 345 1.68e-55 186
MsG0180005131.01.T01 AT4G36880 34.568 324 172 10 76 380 73 375 2.25e-55 186
MsG0180005131.01.T01 AT1G47128 37.584 298 146 9 76 358 72 344 5.25e-55 188
MsG0180005131.01.T01 AT3G19390 36.228 334 163 13 51 363 38 342 3.18e-54 186
MsG0180005131.01.T01 AT2G34080 35.583 326 161 12 55 358 38 336 8.90e-54 181
MsG0180005131.01.T01 AT5G50260 37.124 299 161 7 71 358 52 334 3.09e-53 181
MsG0180005131.01.T01 AT1G29080 36.991 319 169 12 59 363 42 342 5.48e-53 179
MsG0180005131.01.T01 AT1G09850 35.647 317 177 9 56 363 32 330 7.05e-53 181
MsG0180005131.01.T01 AT4G23520 33.746 323 186 9 51 363 42 346 1.09e-52 179
MsG0180005131.01.T01 AT3G48340 35.224 335 172 12 46 358 24 335 1.45e-52 179
MsG0180005131.01.T01 AT4G11320 35.556 315 172 9 56 358 56 351 4.64e-50 172
MsG0180005131.01.T01 AT4G11320 35.556 315 172 9 56 358 56 351 4.64e-50 172
MsG0180005131.01.T01 AT3G49340 33.333 327 175 12 54 361 33 335 8.53e-50 171
MsG0180005131.01.T01 AT3G43960 36.424 302 171 8 56 351 41 327 1.81e-49 171
MsG0180005131.01.T01 AT4G11310 35.312 320 166 10 56 358 49 344 1.87e-49 171
MsG0180005131.01.T01 AT3G48350 31.680 363 209 12 33 377 13 354 2.32e-48 168
MsG0180005131.01.T01 AT2G27420 32.229 332 173 10 54 358 33 339 5.14e-48 166
MsG0180005131.01.T01 AT4G35350 33.571 280 172 4 8 285 7 274 1.13e-47 164
MsG0180005131.01.T01 AT1G29110 33.016 315 166 11 59 358 41 325 3.40e-41 148
MsG0180005131.01.T01 AT3G19400 35.433 254 148 7 38 285 27 270 6.36e-41 146
MsG0180005131.01.T01 AT3G48350 31.321 265 157 8 33 285 13 264 9.94e-34 127
MsG0180005131.01.T01 AT1G02305 27.148 291 167 13 103 366 69 341 7.11e-15 75.9
MsG0180005131.01.T01 AT4G01610 25.532 282 167 12 103 358 66 330 4.47e-14 73.2
MsG0180005131.01.T01 AT4G01610 25.177 282 168 12 103 358 66 330 4.97e-13 70.1
MsG0180005131.01.T01 AT1G02300 26.068 234 135 9 158 369 144 361 8.97e-12 66.2

Find 90 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACTAAATAAGCAGCAATT+TGG 0.276229 1:-87430391 None:intergenic
CACAAGGTTTCTCGATTCTT+AGG 0.295720 1:+87430750 MsG0180005131.01.T01:CDS
GCTTGAGGCTGGTGGATTAG+AGG 0.315848 1:+87430262 MsG0180005131.01.T01:CDS
TTCCCTTGTAGAGTAAGTTT+TGG 0.331460 1:-87429162 None:intergenic
GAATTCTTGATGATCCAATA+TGG 0.338546 1:-87430785 None:intergenic
AGCCTCAAGCAAATAATTAT+AGG 0.341198 1:-87430249 None:intergenic
CCGCCATGAAAACTTGATTC+AGG 0.341418 1:+87429058 MsG0180005131.01.T01:CDS
AACAGAACTAGGTAGCTAAT+AGG 0.375008 1:-87430939 None:intergenic
TGGCGAGGTTGCACCGCCCT+TGG 0.393236 1:+87429358 MsG0180005131.01.T01:CDS
GGCGGTGAAACGCAGTTGAT+AGG 0.395575 1:-87429040 None:intergenic
GGCTTCGCAAGAAACCATAT+TGG 0.397056 1:+87430771 MsG0180005131.01.T01:CDS
CTTGATCCAACCGCAATTCA+TGG 0.398823 1:+87429248 MsG0180005131.01.T01:CDS
TATAATTATTTGCTTGAGGC+TGG 0.400349 1:+87430251 MsG0180005131.01.T01:CDS
GTTCTCATCCACAGGTAGAT+TGG 0.415931 1:-87430369 None:intergenic
AGGGTAAATGTGGATCTTGC+TGG 0.419704 1:+87429841 MsG0180005131.01.T01:intron
ATTCTCTTGCTACATATAAG+AGG 0.422987 1:-87430692 None:intergenic
GGTTGCACCGCCCTTGGATG+TGG 0.428740 1:+87429364 MsG0180005131.01.T01:CDS
CGGTTCTATACCGGTGTTAG+AGG 0.429375 1:+87429311 MsG0180005131.01.T01:CDS
CTTGGATGTGGAAGGATTGC+CGG 0.431739 1:+87429376 MsG0180005131.01.T01:CDS
AAGAGTATTTACTACGCCTT+GGG 0.438150 1:+87429183 MsG0180005131.01.T01:CDS
TGCTGGGCTTTCAGCACTAC+AGG 0.442971 1:+87429858 MsG0180005131.01.T01:CDS
ATACCCGTACACTGGTGGTA+AGG 0.455502 1:+87430295 MsG0180005131.01.T01:CDS
AGCACTACAGGATCTATAGA+AGG 0.470148 1:+87429870 MsG0180005131.01.T01:CDS
GAGTTTGAGCGGTTCTATAC+CGG 0.478459 1:+87429302 MsG0180005131.01.T01:CDS
GAAGAGTATTTACTACGCCT+TGG 0.482670 1:+87429182 MsG0180005131.01.T01:CDS
GAATTTCGATTGGAGGGAGA+AGG 0.483095 1:+87429400 MsG0180005131.01.T01:CDS
AATTTCGATTGGAGGGAGAA+GGG 0.491914 1:+87429401 MsG0180005131.01.T01:CDS
TGCCGGAGAATTTCGATTGG+AGG 0.504986 1:+87429393 MsG0180005131.01.T01:CDS
TCCCTCCAATCGAAATTCTC+CGG 0.505325 1:-87429395 None:intergenic
GCCGGAGAATTTCGATTGGA+GGG 0.506193 1:+87429394 MsG0180005131.01.T01:CDS
CGGCAATCCTTCCACATCCA+AGG 0.507533 1:-87429375 None:intergenic
ACTCCAAAACTTACTCTACA+AGG 0.509081 1:+87429159 MsG0180005131.01.T01:CDS
AACCACGGTGTATTGCTTGT+GGG 0.510752 1:+87430719 MsG0180005131.01.T01:CDS
TACAAGCTTTGCAGAGGGCA+TGG 0.513945 1:+87430839 MsG0180005131.01.T01:CDS
AATTATTTGCTTGAGGCTGG+TGG 0.514952 1:+87430254 MsG0180005131.01.T01:CDS
GCAATTTGGTTCTCATCCAC+AGG 0.515183 1:-87430377 None:intergenic
TTCTATACCGGTGTTAGAGG+TGG 0.515332 1:+87429314 MsG0180005131.01.T01:CDS
TGCCTATAATTATTTGCTTG+AGG 0.515816 1:+87430247 MsG0180005131.01.T01:CDS
GGTGGATTCCCTTCGAGAAA+CGG 0.516124 1:+87429332 MsG0180005131.01.T01:CDS
CGCCAACTCCGTTTCTCGAA+GGG 0.516608 1:-87429340 None:intergenic
GATTGCCGGAGAATTTCGAT+TGG 0.521822 1:+87429390 MsG0180005131.01.T01:CDS
TCATGTGACAATGGTTGTAA+TGG 0.522016 1:+87430209 MsG0180005131.01.T01:CDS
TTCATGCAAACTTACATTGG+TGG 0.523262 1:+87430659 MsG0180005131.01.T01:CDS
TCACCCTTACCACCAGTGTA+CGG 0.527618 1:-87430299 None:intergenic
CACCCTTACCACCAGTGTAC+GGG 0.533213 1:-87430298 None:intergenic
GCTGCTTATTTAGTTAATCA+CGG 0.543083 1:+87430398 MsG0180005131.01.T01:CDS
GCGGTGAAACGCAGTTGATA+GGG 0.543444 1:-87429039 None:intergenic
GGCAATCCTTCCACATCCAA+GGG 0.544410 1:-87429374 None:intergenic
CACAATGGTTTCTGCTGCAA+TGG 0.544762 1:+87430877 MsG0180005131.01.T01:CDS
AATGGTTTCTGCTGCAATGG+TGG 0.547337 1:+87430880 MsG0180005131.01.T01:CDS
GAACCACGGTGTATTGCTTG+TGG 0.550119 1:+87430718 MsG0180005131.01.T01:CDS
CTTGTGGGTTATAATGCACA+AGG 0.551826 1:+87430734 MsG0180005131.01.T01:CDS
ATTTCGATTGGAGGGAGAAG+GGG 0.553540 1:+87429402 MsG0180005131.01.T01:CDS
GCACCGCCCTTGGATGTGGA+AGG 0.555789 1:+87429368 MsG0180005131.01.T01:CDS
GGGTAAATGTGGATCTTGCT+GGG 0.557750 1:+87429842 MsG0180005131.01.T01:intron
TTGCGGTTGGATCAAGTGCT+TGG 0.562169 1:-87429241 None:intergenic
TTCCCTTCGAGAAACGGAGT+TGG 0.564518 1:+87429338 MsG0180005131.01.T01:CDS
TTTCGATTGGAGGGAGAAGG+GGG 0.564832 1:+87429403 MsG0180005131.01.T01:CDS
AAACACGTGAGAGATATGAT+TGG 0.566506 1:-87428984 None:intergenic
TAAGGGGACTTACTTGCAAG+TGG 0.568709 1:-87430422 None:intergenic
TCGCCAACTCCGTTTCTCGA+AGG 0.575720 1:-87429341 None:intergenic
TTGGATCATCAAGAATTCGT+GGG 0.578311 1:+87430790 MsG0180005131.01.T01:CDS
TACCCGTACACTGGTGGTAA+GGG 0.579815 1:+87430296 MsG0180005131.01.T01:CDS
TTCGAGAAACGGAGTTGGCG+AGG 0.581283 1:+87429343 MsG0180005131.01.T01:CDS
TTCTCTTGCTACATATAAGA+GGG 0.587349 1:-87430691 None:intergenic
GTAACACCATGAATTGCGGT+TGG 0.604723 1:-87429254 None:intergenic
ATTGGATCATCAAGAATTCG+TGG 0.604812 1:+87430789 MsG0180005131.01.T01:CDS
TGTTACTGGAGTCAAGATGC+AGG 0.607732 1:+87429427 MsG0180005131.01.T01:CDS
AGGGAATCCACCTCTAACAC+CGG 0.613162 1:-87429321 None:intergenic
TGCAGAGGGCATGGTATGTG+TGG 0.615133 1:+87430848 MsG0180005131.01.T01:CDS
TCTTAGTGAACAACAGCTTG+TGG 0.616431 1:+87429926 MsG0180005131.01.T01:CDS
GGGTATTACAAGCTTTGCAG+AGG 0.620670 1:+87430833 MsG0180005131.01.T01:CDS
GCTTGTGGACTGTGACAGCA+AGG 0.622428 1:+87429941 MsG0180005131.01.T01:CDS
CTCCAAAACTTACTCTACAA+GGG 0.624411 1:+87429160 MsG0180005131.01.T01:CDS
GAAACTTCATACCCGTACAC+TGG 0.628584 1:+87430287 MsG0180005131.01.T01:CDS
GATAAGACATCATGTGACAA+TGG 0.628756 1:+87430200 MsG0180005131.01.T01:CDS
GGTATTACAAGCTTTGCAGA+GGG 0.636304 1:+87430834 MsG0180005131.01.T01:CDS
CTGAGTAACACCATGAATTG+CGG 0.643000 1:-87429258 None:intergenic
TGTGACAATGGTTGTAATGG+AGG 0.643668 1:+87430212 MsG0180005131.01.T01:CDS
GTTCTCTGATCTCTCTGACG+AGG 0.654499 1:+87429280 MsG0180005131.01.T01:CDS
TGAGGCTGGTGGATTAGAGG+AGG 0.654720 1:+87430265 MsG0180005131.01.T01:CDS
ACTTCATACCCGTACACTGG+TGG 0.657388 1:+87430290 MsG0180005131.01.T01:CDS
AATCCTTCCACATCCAAGGG+CGG 0.664048 1:-87429371 None:intergenic
TTGATAGGGTTAGAGCGTAG+AGG 0.669135 1:-87429025 None:intergenic
TGGATCATCAAGAATTCGTG+GGG 0.675621 1:+87430791 MsG0180005131.01.T01:CDS
AAATTTCACCAATCTACCTG+TGG 0.676633 1:+87430361 MsG0180005131.01.T01:CDS
TCTCTGACGAGGAGTTTGAG+CGG 0.687292 1:+87429291 MsG0180005131.01.T01:CDS
AGCAAGAGAATGCTGAACCA+CGG 0.698635 1:+87430704 MsG0180005131.01.T01:CDS
AACCCACAAGCAATACACCG+TGG 0.800815 1:-87430721 None:intergenic
TATGTGTGGAATGAACACAA+TGG 0.864374 1:+87430862 MsG0180005131.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATAAAAAAAAAAAAAA+TGG + Chr1:87429772-87429791 MsG0180005131.01.T01:intron 0.0%
!!! AAATTAAACAGTTTTTATTT+AGG - Chr1:87429815-87429834 None:intergenic 10.0%
!! AAATTAAATAAATTACATCC+CGG - Chr1:87429729-87429748 None:intergenic 15.0%
!! AATGTGATATTTATAGTAAT+AGG + Chr1:87429572-87429591 MsG0180005131.01.T01:intron 15.0%
!! AGAACTTTGTAATTAATTAA+GGG - Chr1:87430442-87430461 None:intergenic 15.0%
!! TAGAACTTTGTAATTAATTA+AGG - Chr1:87430443-87430462 None:intergenic 15.0%
!!! TATTTATGTTTCTAATGATT+AGG + Chr1:87429703-87429722 MsG0180005131.01.T01:intron 15.0%
!!! TTGTTTTTTTATTTACAATG+AGG + Chr1:87429615-87429634 MsG0180005131.01.T01:intron 15.0%
!! AAGTATAGAACATAGTTAAT+TGG - Chr1:87430021-87430040 None:intergenic 20.0%
!! GAACTTTGTAATTAATTAAG+GGG - Chr1:87430441-87430460 None:intergenic 20.0%
!!! AAACTGTTTAATTTTGAAAC+AGG + Chr1:87429821-87429840 MsG0180005131.01.T01:intron 20.0%
!!! AAATCTTGTGTTTTATTAAC+TGG + Chr1:87430131-87430150 MsG0180005131.01.T01:intron 20.0%
!!! AACTGTTTAATTTTGAAACA+GGG + Chr1:87429822-87429841 MsG0180005131.01.T01:intron 20.0%
!!! ATTTTAACTGCAATTTTCTT+TGG - Chr1:87430338-87430357 None:intergenic 20.0%
!!! TTATTTAGGATAATGTTTCA+TGG - Chr1:87429801-87429820 None:intergenic 20.0%
! CAAACTAGAAATTTCAAACA+TGG - Chr1:87429501-87429520 None:intergenic 25.0%
! TAACTAAATAAGCAGCAATT+TGG - Chr1:87430394-87430413 None:intergenic 25.0%
!!! CATGTTTGAAATTTCTAGTT+TGG + Chr1:87429499-87429518 MsG0180005131.01.T01:intron 25.0%
AAGATTTGCAAGAGAAGAAT+TGG - Chr1:87430118-87430137 None:intergenic 30.0%
AGCCTCAAGCAAATAATTAT+AGG - Chr1:87430252-87430271 None:intergenic 30.0%
ATTCTCTTGCTACATATAAG+AGG - Chr1:87430695-87430714 None:intergenic 30.0%
GCTGCTTATTTAGTTAATCA+CGG + Chr1:87430398-87430417 MsG0180005131.01.T01:CDS 30.0%
TATAATTATTTGCTTGAGGC+TGG + Chr1:87430251-87430270 MsG0180005131.01.T01:CDS 30.0%
TGCCTATAATTATTTGCTTG+AGG + Chr1:87430247-87430266 MsG0180005131.01.T01:CDS 30.0%
TTCTCTTGCTACATATAAGA+GGG - Chr1:87430694-87430713 None:intergenic 30.0%
! GAATTCTTGATGATCCAATA+TGG - Chr1:87430788-87430807 None:intergenic 30.0%
! GTTTTCATGCAAACTTACAT+TGG + Chr1:87430656-87430675 MsG0180005131.01.T01:CDS 30.0%
! TATGTTTCTAATGATTAGGC+CGG + Chr1:87429707-87429726 MsG0180005131.01.T01:intron 30.0%
!!! ATTTTGAAACAGGGTAAATG+TGG + Chr1:87429831-87429850 MsG0180005131.01.T01:intron 30.0%
AAACACGTGAGAGATATGAT+TGG - Chr1:87428987-87429006 None:intergenic 35.0%
AAATTTCACCAATCTACCTG+TGG + Chr1:87430361-87430380 MsG0180005131.01.T01:CDS 35.0%
ACTCCAAAACTTACTCTACA+AGG + Chr1:87429159-87429178 MsG0180005131.01.T01:CDS 35.0%
ATTGGATCATCAAGAATTCG+TGG + Chr1:87430789-87430808 MsG0180005131.01.T01:CDS 35.0%
CTCCAAAACTTACTCTACAA+GGG + Chr1:87429160-87429179 MsG0180005131.01.T01:CDS 35.0%
GAGAAAAATGGGGAGAAAAA+GGG + Chr1:87430813-87430832 MsG0180005131.01.T01:CDS 35.0%
GATAAGACATCATGTGACAA+TGG + Chr1:87430200-87430219 MsG0180005131.01.T01:CDS 35.0%
GATGAATTGCGAAACTTAGA+CGG + Chr1:87430479-87430498 MsG0180005131.01.T01:intron 35.0%
TATGTGTGGAATGAACACAA+TGG + Chr1:87430862-87430881 MsG0180005131.01.T01:CDS 35.0%
TCATGTGACAATGGTTGTAA+TGG + Chr1:87430209-87430228 MsG0180005131.01.T01:CDS 35.0%
TGTTATGTCACACTTGACAA+AGG - Chr1:87430180-87430199 None:intergenic 35.0%
TTCATGCAAACTTACATTGG+TGG + Chr1:87430659-87430678 MsG0180005131.01.T01:CDS 35.0%
TTGGATCATCAAGAATTCGT+GGG + Chr1:87430790-87430809 MsG0180005131.01.T01:CDS 35.0%
! ATGTTTCTAATGATTAGGCC+GGG + Chr1:87429708-87429727 MsG0180005131.01.T01:intron 35.0%
! CATCCTGAATCAAGTTTTCA+TGG - Chr1:87429064-87429083 None:intergenic 35.0%
! TGGGATTTTTGCCAAAAACT+TGG + Chr1:87429202-87429221 MsG0180005131.01.T01:CDS 35.0%
! TTCCCTTGTAGAGTAAGTTT+TGG - Chr1:87429165-87429184 None:intergenic 35.0%
! TTTTCCTGTGGCAAGAAAAT+TGG - Chr1:87429898-87429917 None:intergenic 35.0%
!! AAGAGTATTTACTACGCCTT+GGG + Chr1:87429183-87429202 MsG0180005131.01.T01:CDS 35.0%
!!! AGTTTTTGGCAAAAATCCCA+AGG - Chr1:87429202-87429221 None:intergenic 35.0%
!!! GCATATGATTTGTTTTCTGC+TGG - Chr1:87430917-87430936 None:intergenic 35.0%
AACTCTAATCATGTGCAGTG+GGG + Chr1:87430624-87430643 MsG0180005131.01.T01:intron 40.0%
AATTATTTGCTTGAGGCTGG+TGG + Chr1:87430254-87430273 MsG0180005131.01.T01:CDS 40.0%
AATTCGTGGGGAGAAAAATG+GGG + Chr1:87430803-87430822 MsG0180005131.01.T01:CDS 40.0%
AATTTCGATTGGAGGGAGAA+GGG + Chr1:87429401-87429420 MsG0180005131.01.T01:CDS 40.0%
AGAATTCGTGGGGAGAAAAA+TGG + Chr1:87430801-87430820 MsG0180005131.01.T01:CDS 40.0%
AGCACTACAGGATCTATAGA+AGG + Chr1:87429870-87429889 MsG0180005131.01.T01:CDS 40.0%
CAAAAACTTGGTCAAAGCTG+TGG + Chr1:87429214-87429233 MsG0180005131.01.T01:CDS 40.0%
CACAAGGTTTCTCGATTCTT+AGG + Chr1:87430750-87430769 MsG0180005131.01.T01:CDS 40.0%
CTGAGTAACACCATGAATTG+CGG - Chr1:87429261-87429280 None:intergenic 40.0%
GAACTCTAATCATGTGCAGT+GGG + Chr1:87430623-87430642 MsG0180005131.01.T01:intron 40.0%
GAATTCGTGGGGAGAAAAAT+GGG + Chr1:87430802-87430821 MsG0180005131.01.T01:CDS 40.0%
GGAGAAAAATGGGGAGAAAA+AGG + Chr1:87430812-87430831 MsG0180005131.01.T01:CDS 40.0%
GGTATTACAAGCTTTGCAGA+GGG + Chr1:87430834-87430853 MsG0180005131.01.T01:CDS 40.0%
TCTTAGTGAACAACAGCTTG+TGG + Chr1:87429926-87429945 MsG0180005131.01.T01:CDS 40.0%
TGAACTCTAATCATGTGCAG+TGG + Chr1:87430622-87430641 MsG0180005131.01.T01:intron 40.0%
TGGATCATCAAGAATTCGTG+GGG + Chr1:87430791-87430810 MsG0180005131.01.T01:CDS 40.0%
TGTGACAATGGTTGTAATGG+AGG + Chr1:87430212-87430231 MsG0180005131.01.T01:CDS 40.0%
! AAGACTGAGTAGTTTTCCTG+TGG - Chr1:87429910-87429929 None:intergenic 40.0%
! CCTGAATCAAGTTTTCATGG+CGG - Chr1:87429061-87429080 None:intergenic 40.0%
! CTTGTGGGTTATAATGCACA+AGG + Chr1:87430734-87430753 MsG0180005131.01.T01:CDS 40.0%
!! CACAGCTTTGACCAAGTTTT+TGG - Chr1:87429216-87429235 None:intergenic 40.0%
!! GAAGAGTATTTACTACGCCT+TGG + Chr1:87429182-87429201 MsG0180005131.01.T01:CDS 40.0%
AACCACGGTGTATTGCTTGT+GGG + Chr1:87430719-87430738 MsG0180005131.01.T01:CDS 45.0%
AATGGTTTCTGCTGCAATGG+TGG + Chr1:87430880-87430899 MsG0180005131.01.T01:CDS 45.0%
ACTCTAATCATGTGCAGTGG+GGG + Chr1:87430625-87430644 MsG0180005131.01.T01:intron 45.0%
AGCAAGAGAATGCTGAACCA+CGG + Chr1:87430704-87430723 MsG0180005131.01.T01:CDS 45.0%
AGGGTAAATGTGGATCTTGC+TGG + Chr1:87429841-87429860 MsG0180005131.01.T01:intron 45.0%
ATTTCGATTGGAGGGAGAAG+GGG + Chr1:87429402-87429421 MsG0180005131.01.T01:CDS 45.0%
CACAATGGTTTCTGCTGCAA+TGG + Chr1:87430877-87430896 MsG0180005131.01.T01:CDS 45.0%
CCGCCATGAAAACTTGATTC+AGG + Chr1:87429058-87429077 MsG0180005131.01.T01:CDS 45.0%
CTATGTAACACCGACACTTC+AGG - Chr1:87430557-87430576 None:intergenic 45.0%
CTTGATCCAACCGCAATTCA+TGG + Chr1:87429248-87429267 MsG0180005131.01.T01:CDS 45.0%
GAAACTTCATACCCGTACAC+TGG + Chr1:87430287-87430306 MsG0180005131.01.T01:CDS 45.0%
GAATTTCGATTGGAGGGAGA+AGG + Chr1:87429400-87429419 MsG0180005131.01.T01:CDS 45.0%
GAGTTTGAGCGGTTCTATAC+CGG + Chr1:87429302-87429321 MsG0180005131.01.T01:CDS 45.0%
GATTGCCGGAGAATTTCGAT+TGG + Chr1:87429390-87429409 MsG0180005131.01.T01:CDS 45.0%
GGCTTCGCAAGAAACCATAT+TGG + Chr1:87430771-87430790 MsG0180005131.01.T01:CDS 45.0%
GGGTAAATGTGGATCTTGCT+GGG + Chr1:87429842-87429861 MsG0180005131.01.T01:intron 45.0%
GGGTATTACAAGCTTTGCAG+AGG + Chr1:87430833-87430852 MsG0180005131.01.T01:CDS 45.0%
GTAACACCATGAATTGCGGT+TGG - Chr1:87429257-87429276 None:intergenic 45.0%
GTTCTCATCCACAGGTAGAT+TGG - Chr1:87430372-87430391 None:intergenic 45.0%
TAAGGGGACTTACTTGCAAG+TGG - Chr1:87430425-87430444 None:intergenic 45.0%
TCCCTCCAATCGAAATTCTC+CGG - Chr1:87429398-87429417 None:intergenic 45.0%
TGTTACTGGAGTCAAGATGC+AGG + Chr1:87429427-87429446 MsG0180005131.01.T01:CDS 45.0%
TTGATAGGGTTAGAGCGTAG+AGG - Chr1:87429028-87429047 None:intergenic 45.0%
! TTCTATACCGGTGTTAGAGG+TGG + Chr1:87429314-87429333 MsG0180005131.01.T01:CDS 45.0%
!! GCAATTTGGTTCTCATCCAC+AGG - Chr1:87430380-87430399 None:intergenic 45.0%
AACCCACAAGCAATACACCG+TGG - Chr1:87430724-87430743 None:intergenic 50.0%
AATCCTTCCACATCCAAGGG+CGG - Chr1:87429374-87429393 None:intergenic 50.0%
ACTTCATACCCGTACACTGG+TGG + Chr1:87430290-87430309 MsG0180005131.01.T01:CDS 50.0%
AGGGAATCCACCTCTAACAC+CGG - Chr1:87429324-87429343 None:intergenic 50.0%
ATACCCGTACACTGGTGGTA+AGG + Chr1:87430295-87430314 MsG0180005131.01.T01:CDS 50.0%
ATGTGCAGTGGGGGTAAATG+CGG + Chr1:87430634-87430653 MsG0180005131.01.T01:intron 50.0%
CTCCGACAAACCTGAAGTGT+CGG + Chr1:87430544-87430563 MsG0180005131.01.T01:intron 50.0%
CTTGGATGTGGAAGGATTGC+CGG + Chr1:87429376-87429395 MsG0180005131.01.T01:CDS 50.0%
GAACCACGGTGTATTGCTTG+TGG + Chr1:87430718-87430737 MsG0180005131.01.T01:CDS 50.0%
GCCGGAGAATTTCGATTGGA+GGG + Chr1:87429394-87429413 MsG0180005131.01.T01:CDS 50.0%
GGCAATCCTTCCACATCCAA+GGG - Chr1:87429377-87429396 None:intergenic 50.0%
GTTCTCTGATCTCTCTGACG+AGG + Chr1:87429280-87429299 MsG0180005131.01.T01:CDS 50.0%
TACAAGCTTTGCAGAGGGCA+TGG + Chr1:87430839-87430858 MsG0180005131.01.T01:CDS 50.0%
TACCCGTACACTGGTGGTAA+GGG + Chr1:87430296-87430315 MsG0180005131.01.T01:CDS 50.0%
TCACCCTTACCACCAGTGTA+CGG - Chr1:87430302-87430321 None:intergenic 50.0%
TCTCTGACGAGGAGTTTGAG+CGG + Chr1:87429291-87429310 MsG0180005131.01.T01:CDS 50.0%
TGCCGGAGAATTTCGATTGG+AGG + Chr1:87429393-87429412 MsG0180005131.01.T01:CDS 50.0%
TTCCCTTCGAGAAACGGAGT+TGG + Chr1:87429338-87429357 MsG0180005131.01.T01:CDS 50.0%
TTGCGGTTGGATCAAGTGCT+TGG - Chr1:87429244-87429263 None:intergenic 50.0%
TTTCGATTGGAGGGAGAAGG+GGG + Chr1:87429403-87429422 MsG0180005131.01.T01:CDS 50.0%
! CACCGACACTTCAGGTTTGT+CGG - Chr1:87430549-87430568 None:intergenic 50.0%
! CGGTTCTATACCGGTGTTAG+AGG + Chr1:87429311-87429330 MsG0180005131.01.T01:CDS 50.0%
! GCGGTGAAACGCAGTTGATA+GGG - Chr1:87429042-87429061 None:intergenic 50.0%
! GGAGCCAATTTTCTTGCCAC+AGG + Chr1:87429891-87429910 MsG0180005131.01.T01:CDS 50.0%
! GGTGGATTCCCTTCGAGAAA+CGG + Chr1:87429332-87429351 MsG0180005131.01.T01:CDS 50.0%
CACCCTTACCACCAGTGTAC+GGG - Chr1:87430301-87430320 None:intergenic 55.0%
CGCCAACTCCGTTTCTCGAA+GGG - Chr1:87429343-87429362 None:intergenic 55.0%
CGGCAATCCTTCCACATCCA+AGG - Chr1:87429378-87429397 None:intergenic 55.0%
GGCGGTGAAACGCAGTTGAT+AGG - Chr1:87429043-87429062 None:intergenic 55.0%
TCGCCAACTCCGTTTCTCGA+AGG - Chr1:87429344-87429363 None:intergenic 55.0%
TGCTGGGCTTTCAGCACTAC+AGG + Chr1:87429858-87429877 MsG0180005131.01.T01:CDS 55.0%
TTCGAGAAACGGAGTTGGCG+AGG + Chr1:87429343-87429362 MsG0180005131.01.T01:CDS 55.0%
! GCTTGTGGACTGTGACAGCA+AGG + Chr1:87429941-87429960 MsG0180005131.01.T01:CDS 55.0%
! TGCAGAGGGCATGGTATGTG+TGG + Chr1:87430848-87430867 MsG0180005131.01.T01:CDS 55.0%
!! GCTTGAGGCTGGTGGATTAG+AGG + Chr1:87430262-87430281 MsG0180005131.01.T01:CDS 55.0%
!! TGAGGCTGGTGGATTAGAGG+AGG + Chr1:87430265-87430284 MsG0180005131.01.T01:CDS 55.0%
AGGGAGAAGGGGGCTGTTAC+TGG + Chr1:87429413-87429432 MsG0180005131.01.T01:CDS 60.0%
GCACCGCCCTTGGATGTGGA+AGG + Chr1:87429368-87429387 MsG0180005131.01.T01:CDS 65.0%
GGTTGCACCGCCCTTGGATG+TGG + Chr1:87429364-87429383 MsG0180005131.01.T01:CDS 65.0%
TGGCGAGGTTGCACCGCCCT+TGG + Chr1:87429358-87429377 MsG0180005131.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 87428972 87430946 87428972 ID=MsG0180005131.01;Name=MsG0180005131.01
Chr1 mRNA 87428972 87430946 87428972 ID=MsG0180005131.01.T01;Parent=MsG0180005131.01;Name=MsG0180005131.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|4|0|381
Chr1 exon 87428972 87429448 87428972 ID=MsG0180005131.01.T01:exon:47599;Parent=MsG0180005131.01.T01
Chr1 exon 87429843 87429962 87429843 ID=MsG0180005131.01.T01:exon:47600;Parent=MsG0180005131.01.T01
Chr1 exon 87430188 87430431 87430188 ID=MsG0180005131.01.T01:exon:47601;Parent=MsG0180005131.01.T01
Chr1 exon 87430642 87430946 87430642 ID=MsG0180005131.01.T01:exon:47602;Parent=MsG0180005131.01.T01
Chr1 CDS 87428972 87429448 87428972 ID=MsG0180005131.01.T01:cds;Parent=MsG0180005131.01.T01
Chr1 CDS 87429843 87429962 87429843 ID=MsG0180005131.01.T01:cds;Parent=MsG0180005131.01.T01
Chr1 CDS 87430188 87430431 87430188 ID=MsG0180005131.01.T01:cds;Parent=MsG0180005131.01.T01
Chr1 CDS 87430642 87430946 87430642 ID=MsG0180005131.01.T01:cds;Parent=MsG0180005131.01.T01
Gene Sequence

>MsG0180005131.01.T01

ATGGTAACAAAACCAATCATATCTCTCACGTGTTTTGCACGTGTTACAATCTTCCTCTACGCTCTAACCCTATCAACTGCGTTTCACCGCCATGAAAACTTGATTCAGGATGTAGCTAAAAAGCTTGAACTCAAAGACAACGAGCTTCTGAAAACTGAGAAGAAGTTCAAAGTTTTCATGCAAGATTACTCCAAAACTTACTCTACAAGGGAAGAGTATTTACTACGCCTTGGGATTTTTGCCAAAAACTTGGTCAAAGCTGTGGAACACCAAGCACTTGATCCAACCGCAATTCATGGTGTTACTCAGTTCTCTGATCTCTCTGACGAGGAGTTTGAGCGGTTCTATACCGGTGTTAGAGGTGGATTCCCTTCGAGAAACGGAGTTGGCGAGGTTGCACCGCCCTTGGATGTGGAAGGATTGCCGGAGAATTTCGATTGGAGGGAGAAGGGGGCTGTTACTGGAGTCAAGATGCAGGGTAAATGTGGATCTTGCTGGGCTTTCAGCACTACAGGATCTATAGAAGGAGCCAATTTTCTTGCCACAGGAAAACTACTCAGTCTTAGTGAACAACAGCTTGTGGACTGTGACAGCAAGTGTGACATAACAGATAAGACATCATGTGACAATGGTTGTAATGGAGGTCTTATGACAAATGCCTATAATTATTTGCTTGAGGCTGGTGGATTAGAGGAGGAAACTTCATACCCGTACACTGGTGGTAAGGGTGAATGCAAGTTTGATCCAAAGAAAATTGCAGTTAAAATCACAAATTTCACCAATCTACCTGTGGATGAGAACCAAATTGCTGCTTATTTAGTTAATCACGGTCCACTTGCAATGGGGGTAAATGCGGTTTTCATGCAAACTTACATTGGTGGTGTATCATGCCCTCTTATATGTAGCAAGAGAATGCTGAACCACGGTGTATTGCTTGTGGGTTATAATGCACAAGGTTTCTCGATTCTTAGGCTTCGCAAGAAACCATATTGGATCATCAAGAATTCGTGGGGAGAAAAATGGGGAGAAAAAGGGTATTACAAGCTTTGCAGAGGGCATGGTATGTGTGGAATGAACACAATGGTTTCTGCTGCAATGGTGGCTCAAACACAACCAGCAGAAAACAAATCATATGCTTCCTATTAG
Protein sequence

>MsG0180005131.01.T01

MVTKPIISLTCFARVTIFLYALTLSTAFHRHENLIQDVAKKLELKDNELLKTEKKFKVFMQDYSKTYSTREEYLLRLGIFAKNLVKAVEHQALDPTAIHGVTQFSDLSDEEFERFYTGVRGGFPSRNGVGEVAPPLDVEGLPENFDWREKGAVTGVKMQGKCGSCWAFSTTGSIEGANFLATGKLLSLSEQQLVDCDSKCDITDKTSCDNGCNGGLMTNAYNYLLEAGGLEEETSYPYTGGKGECKFDPKKIAVKITNFTNLPVDENQIAAYLVNHGPLAMGVNAVFMQTYIGGVSCPLICSKRMLNHGVLLVGYNAQGFSILRLRKKPYWIIKNSWGEKWGEKGYYKLCRGHGMCGMNTMVSAAMVAQTQPAENKSYASY*