AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048557.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048557.01.T01 MTR_2g081010 70.909 220 9 2 1 165 108 327 1.80e-91 269
MsG0080048557.01.T01 MTR_2g081010 62.353 170 9 2 1 115 108 277 2.02e-60 189
MsG0080048557.01.T01 MTR_2g081010 89.041 73 8 0 1 73 108 180 1.23e-41 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048557.01.T01 AT1G25290 46.190 210 58 2 2 156 119 328 7.83e-58 184
MsG0080048557.01.T01 AT1G25290 64.444 90 32 0 67 156 246 335 1.71e-35 126

Find 88 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCACTTCCTTCCATTAAA+TGG 0.175610 contig354end:-26520 None:intergenic
GCCACATTATAGTTCATTTC+GGG 0.249628 contig354end:-29355 None:intergenic
GTATTTCTCATGCAGCTTAT+TGG 0.251876 contig354end:+28210 MsG0080048557.01.T01:intron
ACATAAGTTAAAGGGGTTTC+AGG 0.262938 contig354end:-29211 None:intergenic
TATTTCTCATGCAGCTTATT+GGG 0.272605 contig354end:+28211 MsG0080048557.01.T01:intron
GAAACATTTCATCTGCCTAT+TGG 0.273856 contig354end:+29076 MsG0080048557.01.T01:exon
AAGTATGCAACATAAGTTAA+AGG 0.285351 contig354end:-29220 None:intergenic
ATTGCTGCATCATGGCTTAT+TGG 0.290926 contig354end:+28942 MsG0080048557.01.T01:CDS
TGCCACATTATAGTTCATTT+CGG 0.296022 contig354end:-29356 None:intergenic
CGCTCCCGTGTTTAGATTGA+AGG 0.319709 contig354end:+26337 MsG0080048557.01.T01:five_prime_UTR
CATCAACTGGCATTGACAAC+TGG 0.321814 contig354end:+28238 MsG0080048557.01.T01:CDS
GCATTTCCAGAGCTGGCTTC+AGG 0.332234 contig354end:-26472 None:intergenic
TATAGTTCATTTCGGGTTGT+AGG 0.358617 contig354end:-29348 None:intergenic
AGTATGCAACATAAGTTAAA+GGG 0.361682 contig354end:-29219 None:intergenic
TCTATTCCGTTCCTGTTTCA+AGG 0.363499 contig354end:+25515 MsG0080048557.01.T01:five_prime_UTR
ACCATTCAAATATTCTCTTA+AGG 0.375492 contig354end:-29177 None:intergenic
AATTCAATCCTTTGAGTTGA+AGG 0.377772 contig354end:-26379 None:intergenic
CTCTTAAGGAAGCTTACAAC+AGG 0.381065 contig354end:-29163 None:intergenic
ACAAGTAATTGCCCTCAATA+TGG 0.389857 contig354end:+27847 MsG0080048557.01.T01:CDS
CATGAATCCACATCTTTAGA+TGG 0.393473 contig354end:+28978 MsG0080048557.01.T01:CDS
AGTGGAGAAGGGAAAGAAAA+TGG 0.394494 contig354end:+25371 MsG0080048557.01.T01:five_prime_UTR
TTTCAGTTAGGAGGCTTGAT+TGG 0.416357 contig354end:+28915 MsG0080048557.01.T01:intron
CTTATTGGGTTATCATCAAC+TGG 0.417736 contig354end:+28225 MsG0080048557.01.T01:CDS
GAAGTGGAGGGACGCGGCGG+TGG 0.420076 contig354end:-25457 None:intergenic
CTGTCACCAGTTTGCAAGGT+GGG 0.426148 contig354end:+25411 MsG0080048557.01.T01:five_prime_UTR
ATCTTGAAGAGATTATATAT+TGG 0.429421 contig354end:-29026 None:intergenic
CCCACGACAAAAGCTTGCCT+TGG 0.437216 contig354end:-27017 None:intergenic
TGAGTCGGCATTTCCAGAGC+TGG 0.439376 contig354end:-26479 None:intergenic
TGTTTCTATTTCCATGGCTT+AGG 0.444460 contig354end:-29059 None:intergenic
TTGCAAACTGGTGACAGAAA+TGG 0.449426 contig354end:-25405 None:intergenic
TTAAGGCACAAAGACCTTGT+AGG 0.451701 contig354end:+27794 MsG0080048557.01.T01:CDS
GGGAGACACGGTGGCTGAAG+TGG 0.457260 contig354end:-25473 None:intergenic
GGAAGACCTGAAGCCAGCTC+TGG 0.461947 contig354end:+26466 MsG0080048557.01.T01:five_prime_UTR
TAAGGACCCACCTTGCAAAC+TGG 0.467629 contig354end:-25417 None:intergenic
GCTTGCGCTTGTAATCCAAT+AGG 0.473399 contig354end:-29091 None:intergenic
TATCTTCACTTGTCAAGTGA+TGG 0.474864 contig354end:+26401 MsG0080048557.01.T01:five_prime_UTR
CCACGACAAAAGCTTGCCTT+GGG 0.477575 contig354end:-27016 None:intergenic
ATGAAATGTTTCTATTTCCA+TGG 0.478226 contig354end:-29065 None:intergenic
TCCTTAAGAGAATATTTGAA+TGG 0.481466 contig354end:+29176 MsG0080048557.01.T01:three_prime_UTR
GGTTTAGGTTGGATCGGTTG+CGG 0.482114 contig354end:+27763 MsG0080048557.01.T01:intron
ATCTTCACTTGTCAAGTGAT+GGG 0.482716 contig354end:+26402 MsG0080048557.01.T01:five_prime_UTR
ATCAACTGGCATTGACAACT+GGG 0.484122 contig354end:+28239 MsG0080048557.01.T01:CDS
GTGGCTGAAGTGGAGGGACG+CGG 0.487609 contig354end:-25463 None:intergenic
TGTTTAGATTGAAGGATAAA+CGG 0.491092 contig354end:+26345 MsG0080048557.01.T01:five_prime_UTR
ATAGTTCATTTCGGGTTGTA+GGG 0.495958 contig354end:-29347 None:intergenic
TGCAAATGTTGGACACCTAA+TGG 0.496467 contig354end:+27194 MsG0080048557.01.T01:CDS
CAGTTAGGAGGCTTGATTGG+TGG 0.501779 contig354end:+28918 MsG0080048557.01.T01:intron
TGCAATGGTTTAGGTTGGAT+CGG 0.506857 contig354end:+27757 MsG0080048557.01.T01:intron
ATGAGACACTTACCATATTG+AGG 0.528872 contig354end:-27859 None:intergenic
ATCCATTTAATGGAAGGAAG+TGG 0.533831 contig354end:+26518 MsG0080048557.01.T01:CDS
ATTTCTGTCACCAGTTTGCA+AGG 0.534725 contig354end:+25407 MsG0080048557.01.T01:five_prime_UTR
AGACACGGTGGCTGAAGTGG+AGG 0.537138 contig354end:-25470 None:intergenic
TCATTGACAAAGGACAACTT+TGG 0.538888 contig354end:+27145 MsG0080048557.01.T01:CDS
GTGGATTCATGCTTCCATGC+AGG 0.542563 contig354end:-28966 None:intergenic
TTGGTGGAATTGCTGCATCA+TGG 0.545395 contig354end:+28934 MsG0080048557.01.T01:CDS
GCAATGATATCAAGTGGGTA+AGG 0.550443 contig354end:-25435 None:intergenic
CACAAAGACCTTGTAGGAGG+TGG 0.557075 contig354end:+27800 MsG0080048557.01.T01:CDS
TCTTCTTCCATCTAAAGATG+TGG 0.558989 contig354end:-28985 None:intergenic
CAAAGATACCTTCAACTCAA+AGG 0.560846 contig354end:+26371 MsG0080048557.01.T01:five_prime_UTR
TGAGACACTTACCATATTGA+GGG 0.563701 contig354end:-27858 None:intergenic
ACTGGTGACAGAAATGGCTT+CGG 0.565259 contig354end:-25399 None:intergenic
TTAAGAGAATATTTGAATGG+TGG 0.565446 contig354end:+29179 MsG0080048557.01.T01:three_prime_UTR
CTCACAAAACATTGGCAGCA+AGG 0.571689 contig354end:-26552 None:intergenic
GGCGGAGACGGTGGGAGACA+CGG 0.580809 contig354end:-25485 None:intergenic
AAACTCGAGGCGGAGACGGT+GGG 0.585572 contig354end:-25493 None:intergenic
GTTGCGCAACTTGCAACCCA+AGG 0.593062 contig354end:+27000 MsG0080048557.01.T01:CDS
TTACGAGAGTACCTAAGCCA+TGG 0.600438 contig354end:+29048 MsG0080048557.01.T01:CDS
GACACGGTGGCTGAAGTGGA+GGG 0.603040 contig354end:-25469 None:intergenic
GAAACTCGAGGCGGAGACGG+TGG 0.603422 contig354end:-25494 None:intergenic
TGCTCATACTCACAAAACAT+TGG 0.609078 contig354end:-26560 None:intergenic
CATGGCTTATTGGACCTGCA+TGG 0.609641 contig354end:+28952 MsG0080048557.01.T01:CDS
GTATGCAACATAAGTTAAAG+GGG 0.609786 contig354end:-29218 None:intergenic
GCGGTGGCAATGATATCAAG+TGG 0.623394 contig354end:-25441 None:intergenic
TCTGTCACCAGTTTGCAAGG+TGG 0.623907 contig354end:+25410 MsG0080048557.01.T01:five_prime_UTR
TTATCCTTCAATCTAAACAC+GGG 0.626143 contig354end:-26341 None:intergenic
AGGCACAAAGACCTTGTAGG+AGG 0.629028 contig354end:+27797 MsG0080048557.01.T01:CDS
ATAGAAACTCGAGGCGGAGA+CGG 0.632059 contig354end:-25497 None:intergenic
GTAAACAGTCTCATTGACAA+AGG 0.638755 contig354end:+27135 MsG0080048557.01.T01:CDS
ACCCGAAATGAACTATAATG+TGG 0.645840 contig354end:+29354 MsG0080048557.01.T01:three_prime_UTR
GCTGAAGTGGAGGGACGCGG+CGG 0.649712 contig354end:-25460 None:intergenic
AGGAGACCTTGAAACAGGAA+CGG 0.658591 contig354end:-25521 None:intergenic
CGGTGGCAATGATATCAAGT+GGG 0.662467 contig354end:-25440 None:intergenic
TTTATCCTTCAATCTAAACA+CGG 0.664210 contig354end:-26342 None:intergenic
GGAGACGGTGGGAGACACGG+TGG 0.711069 contig354end:-25482 None:intergenic
AGGAACGGAATAGAAACTCG+AGG 0.720325 contig354end:-25506 None:intergenic
TCAACTGGCATTGACAACTG+GGG 0.722807 contig354end:+28240 MsG0080048557.01.T01:CDS
TCTTAAGGAAGCTTACAACA+GGG 0.725124 contig354end:-29162 None:intergenic
AACGGAATAGAAACTCGAGG+CGG 0.761340 contig354end:-25503 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTTATTTTTAAA+TGG - contig354end:27629-27648 None:intergenic 0.0%
!! TAAGAAAATGAAAAATTTAA+GGG - contig354end:28309-28328 None:intergenic 10.0%
!!! TATATTTTTTCTTCTTATTT+AGG + contig354end:28042-28061 MsG0080048557.01.T01:intron 10.0%
!! AATTATGGAATTAGAATATT+TGG - contig354end:27447-27466 None:intergenic 15.0%
!! AGTAAAAAGAGAAAATAAAA+AGG - contig354end:25544-25563 None:intergenic 15.0%
!! CTAAGAAAATGAAAAATTTA+AGG - contig354end:28310-28329 None:intergenic 15.0%
!!! ATTATTGATTAACAAATCAA+AGG - contig354end:26731-26750 None:intergenic 15.0%
!!! TTTTATTTTTAAATGGTTCA+GGG - contig354end:27622-27641 None:intergenic 15.0%
!!! TTTTTATTTTTAAATGGTTC+AGG - contig354end:27623-27642 None:intergenic 15.0%
!! AATTCAATTACCTTTAACAT+GGG + contig354end:29261-29280 MsG0080048557.01.T01:intron 20.0%
!! ATCTTGAAGAGATTATATAT+TGG - contig354end:29029-29048 None:intergenic 20.0%
!! CTGAAAATTGTATATGTTAA+TGG - contig354end:28901-28920 None:intergenic 20.0%
!! TAATTCAATTACCTTTAACA+TGG + contig354end:29260-29279 MsG0080048557.01.T01:intron 20.0%
!! TATTGTAGGAATAATATAAG+CGG - contig354end:27404-27423 None:intergenic 20.0%
!! TTATTTAGGAAATGTTTGTT+CGG + contig354end:28056-28075 MsG0080048557.01.T01:intron 20.0%
!!! AACATATACAATTTTCAGTT+AGG + contig354end:28903-28922 MsG0080048557.01.T01:intron 20.0%
!!! AGCTAGTTTTCTTTTTTTAA+TGG + contig354end:26442-26461 MsG0080048557.01.T01:five_prime_UTR 20.0%
!!! CTTTATTCACTTTTAAAATG+TGG + contig354end:28386-28405 MsG0080048557.01.T01:intron 20.0%
!!! GTATCTAAAATTGTAAAAAG+AGG - contig354end:26874-26893 None:intergenic 20.0%
!!! TAGTTTTCTTTTTTTAATGG+AGG + contig354end:26445-26464 MsG0080048557.01.T01:five_prime_UTR 20.0%
!!! TTTATTTTTAAATGGTTCAG+GGG - contig354end:27621-27640 None:intergenic 20.0%
!!! TTTTAATACAAAACTAAACC+AGG - contig354end:27260-27279 None:intergenic 20.0%
! AAACTTAATACACCAATCAA+GGG - contig354end:26590-26609 None:intergenic 25.0%
! AAGTATGCAACATAAGTTAA+AGG - contig354end:29223-29242 None:intergenic 25.0%
! AATACAGTGAAGAAAGATAA+AGG - contig354end:27727-27746 None:intergenic 25.0%
! AATTAGAATATTTGGAGGTA+AGG - contig354end:27439-27458 None:intergenic 25.0%
! ACCATTCAAATATTCTCTTA+AGG - contig354end:29180-29199 None:intergenic 25.0%
! ACTTAAATACTCCATTTCAA+TGG - contig354end:25650-25669 None:intergenic 25.0%
! AGTATGCAACATAAGTTAAA+GGG - contig354end:29222-29241 None:intergenic 25.0%
! AGTGAAAAGAAAGAAAAACA+AGG + contig354end:26654-26673 MsG0080048557.01.T01:intron 25.0%
! ATGATACAGTCACTTAATTA+AGG - contig354end:25968-25987 None:intergenic 25.0%
! ATTCAATTACCTTTAACATG+GGG + contig354end:29262-29281 MsG0080048557.01.T01:intron 25.0%
! CAGAAAAATGAAGAGAAAAA+TGG - contig354end:26826-26845 None:intergenic 25.0%
! GAAAAAATCCATTTAATGGA+AGG + contig354end:26512-26531 MsG0080048557.01.T01:CDS 25.0%
! GACTTGTATCAAGTAAAATA+AGG - contig354end:27599-27618 None:intergenic 25.0%
! GTGAAAAGAAAGAAAAACAA+GGG + contig354end:26655-26674 MsG0080048557.01.T01:intron 25.0%
! TAAACACTTATGAAACTGTT+TGG - contig354end:28670-28689 None:intergenic 25.0%
! TAAACTTAATACACCAATCA+AGG - contig354end:26591-26610 None:intergenic 25.0%
! TAAGTTGTTTATCTTAGTTG+AGG - contig354end:28462-28481 None:intergenic 25.0%
! TACTACACATTGCAAAAATT+TGG + contig354end:25560-25579 MsG0080048557.01.T01:intron 25.0%
! TATGGAATTAGAATATTTGG+AGG - contig354end:27444-27463 None:intergenic 25.0%
! TCCAGAAAAAATCCATTTAA+TGG + contig354end:26508-26527 MsG0080048557.01.T01:CDS 25.0%
! TCCTTAAGAGAATATTTGAA+TGG + contig354end:29176-29195 MsG0080048557.01.T01:three_prime_UTR 25.0%
! TGTTTAGATTGAAGGATAAA+CGG + contig354end:26345-26364 MsG0080048557.01.T01:five_prime_UTR 25.0%
! TTAAGAGAATATTTGAATGG+TGG + contig354end:29179-29198 MsG0080048557.01.T01:three_prime_UTR 25.0%
! TTAATGCTGATGATTAAACT+GGG + contig354end:25707-25726 MsG0080048557.01.T01:intron 25.0%
! TTAATTCAAATCCTACCATT+AGG - contig354end:27212-27231 None:intergenic 25.0%
! TTTATCCTTCAATCTAAACA+CGG - contig354end:26345-26364 None:intergenic 25.0%
!! AGTTTTCTATCTTCTCTATA+TGG - contig354end:27693-27712 None:intergenic 25.0%
!! ATATACAATTTTCAGTTAGG+AGG + contig354end:28906-28925 MsG0080048557.01.T01:intron 25.0%
!! ATGAAATGTTTCTATTTCCA+TGG - contig354end:29068-29087 None:intergenic 25.0%
!! TCCATAATTTCTAGAAAATC+AGG + contig354end:27458-27477 MsG0080048557.01.T01:intron 25.0%
!! TTACAATTTTAGATACCTCT+TGG + contig354end:26878-26897 MsG0080048557.01.T01:intron 25.0%
!!! AAAATGGATAGTCTTATACA+CGG - contig354end:28787-28806 None:intergenic 25.0%
!!! ACCTGATTTTCTAGAAATTA+TGG - contig354end:27462-27481 None:intergenic 25.0%
!!! ATCCATTTTGTTATGTATGT+AGG + contig354end:28798-28817 MsG0080048557.01.T01:intron 25.0%
!!! TCCATTAAATGGATTTTTTC+TGG - contig354end:26512-26531 None:intergenic 25.0%
AAACTATGCACAGATTACAT+TGG - contig354end:28858-28877 None:intergenic 30.0%
AACAAGCTGATAACAACTTA+TGG + contig354end:28615-28634 MsG0080048557.01.T01:intron 30.0%
AATAGATATGATCTGTGTTG+TGG + contig354end:27076-27095 MsG0080048557.01.T01:intron 30.0%
AATTAAGGATCTGTTTGGAT+TGG - contig354end:25953-25972 None:intergenic 30.0%
ACATTGCAAAAATTTGGACT+CGG + contig354end:25566-25585 MsG0080048557.01.T01:intron 30.0%
AGAAGAAAGATACACAATTG+GGG - contig354end:26256-26275 None:intergenic 30.0%
ATACACGGAATACAAAATTG+AGG - contig354end:28772-28791 None:intergenic 30.0%
ATAGAAAGGAACCATTGAAA+TGG + contig354end:25636-25655 MsG0080048557.01.T01:intron 30.0%
CACTTAATTAAGGATCTGTT+TGG - contig354end:25958-25977 None:intergenic 30.0%
CAGAAGAAAGATACACAATT+GGG - contig354end:26257-26276 None:intergenic 30.0%
CAGTACAAACTATTCAAATC+CGG - contig354end:26803-26822 None:intergenic 30.0%
GTATGCAACATAAGTTAAAG+GGG - contig354end:29221-29240 None:intergenic 30.0%
GTTAATGCTGATGATTAAAC+TGG + contig354end:25706-25725 MsG0080048557.01.T01:intron 30.0%
GTTTATGACTTCCTAGAATT+TGG + contig354end:26606-26625 MsG0080048557.01.T01:intron 30.0%
TAAATTGCAAGATATCTTCG+AGG - contig354end:27357-27376 None:intergenic 30.0%
TATGGATGTGACTTATAGAT+GGG + contig354end:28148-28167 MsG0080048557.01.T01:intron 30.0%
TATTTCTCATGCAGCTTATT+GGG + contig354end:28211-28230 MsG0080048557.01.T01:intron 30.0%
TCAAAAAAGTCAATCCAAAC+AGG + contig354end:28704-28723 MsG0080048557.01.T01:intron 30.0%
TCAGAAGAAAGATACACAAT+TGG - contig354end:26258-26277 None:intergenic 30.0%
TCCTTTCTATAACCTAAATG+AGG - contig354end:25626-25645 None:intergenic 30.0%
TCTATATGGAAAACCAAGAT+AGG - contig354end:27679-27698 None:intergenic 30.0%
TGCCACATTATAGTTCATTT+CGG - contig354end:29359-29378 None:intergenic 30.0%
TGCCTACATACATAACAAAA+TGG - contig354end:28803-28822 None:intergenic 30.0%
TGTAAAATTTATGTCCACTG+TGG - contig354end:27978-27997 None:intergenic 30.0%
TGTTACTCCTTAAATTCAGT+TGG + contig354end:27306-27325 MsG0080048557.01.T01:intron 30.0%
TTATCCTTCAATCTAAACAC+GGG - contig354end:26344-26363 None:intergenic 30.0%
! TAGTTCTCTTTTCCTCATTT+AGG + contig354end:25611-25630 MsG0080048557.01.T01:intron 30.0%
! TCCTCATTTAGGTTATAGAA+AGG + contig354end:25622-25641 MsG0080048557.01.T01:intron 30.0%
!! AAAGACTTGTTAGATTGTTC+AGG - contig354end:26194-26213 None:intergenic 30.0%
!! AAGACTTGTTAGATTGTTCA+GGG - contig354end:26193-26212 None:intergenic 30.0%
!! AATTCAATCCTTTGAGTTGA+AGG - contig354end:26382-26401 None:intergenic 30.0%
!! CTTGTTTTCATAAGCTATCT+TGG + contig354end:25819-25838 MsG0080048557.01.T01:intron 30.0%
!! TGTATCTTTCTTCTGAAATC+TGG + contig354end:26262-26281 MsG0080048557.01.T01:intron 30.0%
AAAAATCATCCATACAGGCA+AGG - contig354end:29313-29332 None:intergenic 35.0%
AAACAGGCCTAAAAACTATG+TGG + contig354end:28720-28739 MsG0080048557.01.T01:intron 35.0%
AAGATAGAAAACTGAGATGC+TGG + contig354end:27700-27719 MsG0080048557.01.T01:intron 35.0%
AAGGGCAGTAAAAAAATCCA+TGG - contig354end:28291-28310 None:intergenic 35.0%
AATAGGAAGTAGCTCATGTT+TGG + contig354end:25730-25749 MsG0080048557.01.T01:intron 35.0%
ACAAGTAATTGCCCTCAATA+TGG + contig354end:27847-27866 MsG0080048557.01.T01:CDS 35.0%
ACATAAGTTAAAGGGGTTTC+AGG - contig354end:29214-29233 None:intergenic 35.0%
ACCCAAAAAATCATCCATAC+AGG - contig354end:29318-29337 None:intergenic 35.0%
ACCCGAAATGAACTATAATG+TGG + contig354end:29354-29373 MsG0080048557.01.T01:three_prime_UTR 35.0%
AGAAAGCAAAGTGAGCATTA+TGG - contig354end:26754-26773 None:intergenic 35.0%
ATCCATTTAATGGAAGGAAG+TGG + contig354end:26518-26537 MsG0080048557.01.T01:CDS 35.0%
ATCTTCACTTGTCAAGTGAT+GGG + contig354end:26402-26421 MsG0080048557.01.T01:five_prime_UTR 35.0%
ATGAGACACTTACCATATTG+AGG - contig354end:27862-27881 None:intergenic 35.0%
CAAAGATACCTTCAACTCAA+AGG + contig354end:26371-26390 MsG0080048557.01.T01:five_prime_UTR 35.0%
CATAAAGATCCCCATGTTAA+AGG - contig354end:29274-29293 None:intergenic 35.0%
CATGAATCCACATCTTTAGA+TGG + contig354end:28978-28997 MsG0080048557.01.T01:CDS 35.0%
CCTTTGTTCAGTAAAAGTTG+CGG + contig354end:28332-28351 MsG0080048557.01.T01:intron 35.0%
CTATGGATGTGACTTATAGA+TGG + contig354end:28147-28166 MsG0080048557.01.T01:intron 35.0%
CTGATGATTAAACTGGGAAT+AGG + contig354end:25713-25732 MsG0080048557.01.T01:intron 35.0%
CTTATTGGGTTATCATCAAC+TGG + contig354end:28225-28244 MsG0080048557.01.T01:CDS 35.0%
CTTTGTTCAGTAAAAGTTGC+GGG + contig354end:28333-28352 MsG0080048557.01.T01:intron 35.0%
GAAACATTTCATCTGCCTAT+TGG + contig354end:29076-29095 MsG0080048557.01.T01:exon 35.0%
GAAACTGTTTGGAAGACTTA+TGG - contig354end:28659-28678 None:intergenic 35.0%
GCCACATTATAGTTCATTTC+GGG - contig354end:29358-29377 None:intergenic 35.0%
GCTTATTTGAGCTTATCTAC+TGG - contig354end:25931-25950 None:intergenic 35.0%
GTAAACAGTCTCATTGACAA+AGG + contig354end:27135-27154 MsG0080048557.01.T01:CDS 35.0%
GTATTTCTCATGCAGCTTAT+TGG + contig354end:28210-28229 MsG0080048557.01.T01:intron 35.0%
GTTGTGTTGTGTATGTTACT+AGG + contig354end:27113-27132 MsG0080048557.01.T01:intron 35.0%
TAAAAAGGAGACCTTGAAAC+AGG - contig354end:25529-25548 None:intergenic 35.0%
TAACTTAGACCATCACTCTA+TGG + contig354end:28130-28149 MsG0080048557.01.T01:intron 35.0%
TATCTTCACTTGTCAAGTGA+TGG + contig354end:26401-26420 MsG0080048557.01.T01:five_prime_UTR 35.0%
TATGAGCATATTCCCTTGAT+TGG + contig354end:26575-26594 MsG0080048557.01.T01:intron 35.0%
TCATTGACAAAGGACAACTT+TGG + contig354end:27145-27164 MsG0080048557.01.T01:CDS 35.0%
TCTTAAGGAAGCTTACAACA+GGG - contig354end:29165-29184 None:intergenic 35.0%
TCTTCTTCCATCTAAAGATG+TGG - contig354end:28988-29007 None:intergenic 35.0%
TGAGACACTTACCATATTGA+GGG - contig354end:27861-27880 None:intergenic 35.0%
TGCTCATACTCACAAAACAT+TGG - contig354end:26563-26582 None:intergenic 35.0%
TGTTTGTTTACGATACCTCT+TGG + contig354end:26928-26947 MsG0080048557.01.T01:intron 35.0%
TTGGAGGTAAGGAATTTCAT+TGG - contig354end:27428-27447 None:intergenic 35.0%
TTTCCTTGTCTGTGTTCTAA+CGG + contig354end:28182-28201 MsG0080048557.01.T01:intron 35.0%
TTTCTATCATGATGAGCTTC+TGG + contig354end:28591-28610 MsG0080048557.01.T01:intron 35.0%
! GGAATTTCATTGGCTATTGT+AGG - contig354end:27418-27437 None:intergenic 35.0%
! GTATTTCTAATGAGCTGCAA+TGG + contig354end:27742-27761 MsG0080048557.01.T01:intron 35.0%
! TCCATGTCCACATAGTTTTT+AGG - contig354end:28730-28749 None:intergenic 35.0%
! TCTTTTCTGCATGCAAATGT+TGG + contig354end:27183-27202 MsG0080048557.01.T01:CDS 35.0%
! TGTTTCTATTTCCATGGCTT+AGG - contig354end:29062-29081 None:intergenic 35.0%
! TTTGTTATGTATGTAGGCAC+TGG + contig354end:28804-28823 MsG0080048557.01.T01:intron 35.0%
! TTTTCTGGACATCTTTGAGT+CGG - contig354end:26497-26516 None:intergenic 35.0%
!! ACATAGTTTTTAGGCCTGTT+TGG - contig354end:28721-28740 None:intergenic 35.0%
!! AGACTTGTTAGATTGTTCAG+GGG - contig354end:26192-26211 None:intergenic 35.0%
!! ATAGTTCATTTCGGGTTGTA+GGG - contig354end:29350-29369 None:intergenic 35.0%
!! TATAGTTCATTTCGGGTTGT+AGG - contig354end:29351-29370 None:intergenic 35.0%
!!! ATGGATGATTTTTTGGGTCT+AGG + contig354end:29320-29339 MsG0080048557.01.T01:three_prime_UTR 35.0%
!!! GCCTGTATGGATGATTTTTT+GGG + contig354end:29314-29333 MsG0080048557.01.T01:three_prime_UTR 35.0%
!!! TGCCTGTATGGATGATTTTT+TGG + contig354end:29313-29332 MsG0080048557.01.T01:three_prime_UTR 35.0%
!!! TGTTAGTTTTGCAACTTCAC+TGG + contig354end:27908-27927 MsG0080048557.01.T01:intron 35.0%
!!! TTTTAAAATGTGGCATGACC+TGG + contig354end:28396-28415 MsG0080048557.01.T01:intron 35.0%
AAGTCACATCCATAGAGTGA+TGG - contig354end:28142-28161 None:intergenic 40.0%
AATGTTGGACACCTAATGGT+AGG + contig354end:27198-27217 MsG0080048557.01.T01:intron 40.0%
AGCAGAATCAGAAGTAACAC+AGG - contig354end:27887-27906 None:intergenic 40.0%
AGTGGAGAAGGGAAAGAAAA+TGG + contig354end:25371-25390 MsG0080048557.01.T01:five_prime_UTR 40.0%
AGTGGGACCAACTGAATTTA+AGG - contig354end:27316-27335 None:intergenic 40.0%
ATTGCTGCATCATGGCTTAT+TGG + contig354end:28942-28961 MsG0080048557.01.T01:CDS 40.0%
ATTTCTGTCACCAGTTTGCA+AGG + contig354end:25407-25426 MsG0080048557.01.T01:five_prime_UTR 40.0%
CGAGTTTCTGTTCAACAAGA+GGG - contig354end:26319-26338 None:intergenic 40.0%
CTAATGAGCTGCAATGGTTT+AGG + contig354end:27748-27767 MsG0080048557.01.T01:intron 40.0%
CTCTTAAGGAAGCTTACAAC+AGG - contig354end:29166-29185 None:intergenic 40.0%
GCAATGATATCAAGTGGGTA+AGG - contig354end:25438-25457 None:intergenic 40.0%
GCAGAATCAGAAGTAACACA+GGG - contig354end:27886-27905 None:intergenic 40.0%
GCCTAAAAACTATGTGGACA+TGG + contig354end:28726-28745 MsG0080048557.01.T01:intron 40.0%
GTCCACTTCCTTCCATTAAA+TGG - contig354end:26523-26542 None:intergenic 40.0%
TCAAACGTACTTGTGTCTCT+TGG + contig354end:27650-27669 MsG0080048557.01.T01:intron 40.0%
TCTATTCCGTTCCTGTTTCA+AGG + contig354end:25515-25534 MsG0080048557.01.T01:five_prime_UTR 40.0%
TGCAAATGTTGGACACCTAA+TGG + contig354end:27194-27213 MsG0080048557.01.T01:CDS 40.0%
TGTCTCTTGGATTCCTATCT+TGG + contig354end:27663-27682 MsG0080048557.01.T01:intron 40.0%
TTAAGGCACAAAGACCTTGT+AGG + contig354end:27794-27813 MsG0080048557.01.T01:CDS 40.0%
TTACACTCCACTCACTTTAG+AGG - contig354end:26634-26653 None:intergenic 40.0%
TTGCAAACTGGTGACAGAAA+TGG - contig354end:25408-25427 None:intergenic 40.0%
TTTCAGTTAGGAGGCTTGAT+TGG + contig354end:28915-28934 MsG0080048557.01.T01:intron 40.0%
! CCGCAACTTTTACTGAACAA+AGG - contig354end:28335-28354 None:intergenic 40.0%
! CTCGAATTTAGAGTCATTCC+AGG - contig354end:28417-28436 None:intergenic 40.0%
! CTTTAGAGGAGCCAAATTCT+AGG - contig354end:26620-26639 None:intergenic 40.0%
!! AAGTGTTGTTGGTTCATGCA+AGG + contig354end:28082-28101 MsG0080048557.01.T01:intron 40.0%
!! AGTGTTGTTGGTTCATGCAA+GGG + contig354end:28083-28102 MsG0080048557.01.T01:intron 40.0%
!! ATCAACTGGCATTGACAACT+GGG + contig354end:28239-28258 MsG0080048557.01.T01:CDS 40.0%
!! GTTTGTTTTCCTTGCCTGTA+TGG + contig354end:29301-29320 MsG0080048557.01.T01:three_prime_UTR 40.0%
!! TGCAATGGTTTAGGTTGGAT+CGG + contig354end:27757-27776 MsG0080048557.01.T01:intron 40.0%
!! TTGTTCGGTAGAAGTGTTGT+TGG + contig354end:28071-28090 MsG0080048557.01.T01:intron 40.0%
!!! CTTTTTTACCACCTCCTACA+AGG - contig354end:27811-27830 None:intergenic 40.0%
AAATTCAGTTGGTCCCACTG+TGG + contig354end:27317-27336 MsG0080048557.01.T01:intron 45.0%
AACGGAATAGAAACTCGAGG+CGG - contig354end:25506-25525 None:intergenic 45.0%
AACTGGGGACATGTAAGTGT+AGG + contig354end:28255-28274 MsG0080048557.01.T01:intron 45.0%
ACTGGTGACAGAAATGGCTT+CGG - contig354end:25402-25421 None:intergenic 45.0%
AGAGATCTACTTCTCCTTCG+CGG + contig354end:29386-29405 MsG0080048557.01.T01:three_prime_UTR 45.0%
AGGAACGGAATAGAAACTCG+AGG - contig354end:25509-25528 None:intergenic 45.0%
AGGAGACCTTGAAACAGGAA+CGG - contig354end:25524-25543 None:intergenic 45.0%
CAATAAGCACTCTCACCAAG+AGG - contig354end:26896-26915 None:intergenic 45.0%
CAGGCATGACAGAACAAACT+TGG - contig354end:27241-27260 None:intergenic 45.0%
CAGGTGCAGCTATGAGTTAT+TGG + contig354end:27477-27496 MsG0080048557.01.T01:intron 45.0%
CGGTGGCAATGATATCAAGT+GGG - contig354end:25443-25462 None:intergenic 45.0%
CTCACAAAACATTGGCAGCA+AGG - contig354end:26555-26574 None:intergenic 45.0%
GCGAGTTTCTGTTCAACAAG+AGG - contig354end:26320-26339 None:intergenic 45.0%
GCGGTTACTTGCAATTGAAG+AGG - contig354end:27385-27404 None:intergenic 45.0%
GCTTGCGCTTGTAATCCAAT+AGG - contig354end:29094-29113 None:intergenic 45.0%
GGTTCATGCAAGGGTTATGT+CGG + contig354end:28092-28111 MsG0080048557.01.T01:intron 45.0%
GTTCATGCAAGGGTTATGTC+GGG + contig354end:28093-28112 MsG0080048557.01.T01:intron 45.0%
TGAGCTGCAATGGTTTAGGT+TGG + contig354end:27752-27771 MsG0080048557.01.T01:intron 45.0%
TTCTGCAAAATGCCTGCAGT+AGG + contig354end:27500-27519 MsG0080048557.01.T01:intron 45.0%
TTGGTGGAATTGCTGCATCA+TGG + contig354end:28934-28953 MsG0080048557.01.T01:CDS 45.0%
! ATGCTTCTGATTAGCCACAG+TGG + contig354end:27961-27980 MsG0080048557.01.T01:intron 45.0%
! CAAGTTTGTTCTGTCATGCC+TGG + contig354end:27239-27258 MsG0080048557.01.T01:intron 45.0%
! GTGTAGGAGTGTATCATCCA+TGG + contig354end:28271-28290 MsG0080048557.01.T01:intron 45.0%
!! CATCAACTGGCATTGACAAC+TGG + contig354end:28238-28257 MsG0080048557.01.T01:CDS 45.0%
!! TCAACTGGCATTGACAACTG+GGG + contig354end:28240-28259 MsG0080048557.01.T01:CDS 45.0%
!! TTACGAGAGTACCTAAGCCA+TGG + contig354end:29048-29067 MsG0080048557.01.T01:CDS 45.0%
!! TTTGGCTCCTCTAAAGTGAG+TGG + contig354end:26624-26643 MsG0080048557.01.T01:intron 45.0%
!!! CGGTTGCGGTGTTTGTTTTA+AGG + contig354end:27777-27796 MsG0080048557.01.T01:CDS 45.0%
ACCACTAAAGCTCTCCACAG+TGG - contig354end:27334-27353 None:intergenic 50.0%
AGGCACAAAGACCTTGTAGG+AGG + contig354end:27797-27816 MsG0080048557.01.T01:CDS 50.0%
AGTTGTCACGAGACAGTGTG+TGG + contig354end:27935-27954 MsG0080048557.01.T01:intron 50.0%
ATAGAAACTCGAGGCGGAGA+CGG - contig354end:25500-25519 None:intergenic 50.0%
CAAGAGGGACATGTGAGTCA+AGG - contig354end:26304-26323 None:intergenic 50.0%
CACAAAGACCTTGTAGGAGG+TGG + contig354end:27800-27819 MsG0080048557.01.T01:CDS 50.0%
CAGTTAGGAGGCTTGATTGG+TGG + contig354end:28918-28937 MsG0080048557.01.T01:intron 50.0%
CATGGCTTATTGGACCTGCA+TGG + contig354end:28952-28971 MsG0080048557.01.T01:CDS 50.0%
CCACGACAAAAGCTTGCCTT+GGG - contig354end:27019-27038 None:intergenic 50.0%
CCACTAAAGCTCTCCACAGT+GGG - contig354end:27333-27352 None:intergenic 50.0%
CGCTCCCGTGTTTAGATTGA+AGG + contig354end:26337-26356 MsG0080048557.01.T01:five_prime_UTR 50.0%
CTGTCACCAGTTTGCAAGGT+GGG + contig354end:25411-25430 MsG0080048557.01.T01:five_prime_UTR 50.0%
GCACCGTTAGAACACAGACA+AGG - contig354end:28188-28207 None:intergenic 50.0%
GCGGTGGCAATGATATCAAG+TGG - contig354end:25444-25463 None:intergenic 50.0%
GTGGATTCATGCTTCCATGC+AGG - contig354end:28969-28988 None:intergenic 50.0%
GTTGCTCGCTTCAAATGAGC+CGG + contig354end:26781-26800 MsG0080048557.01.T01:intron 50.0%
TAAGGACCCACCTTGCAAAC+TGG - contig354end:25420-25439 None:intergenic 50.0%
TCTGTCACCAGTTTGCAAGG+TGG + contig354end:25410-25429 MsG0080048557.01.T01:five_prime_UTR 50.0%
! CAAGGCAAGCTTTTGTCGTG+GGG + contig354end:27018-27037 MsG0080048557.01.T01:CDS 50.0%
! CCAAGGCAAGCTTTTGTCGT+GGG + contig354end:27017-27036 MsG0080048557.01.T01:CDS 50.0%
! GGTGCGTCAGGAGCAATTTT+TGG + contig354end:27521-27540 MsG0080048557.01.T01:intron 50.0%
!! GGTTTAGGTTGGATCGGTTG+CGG + contig354end:27763-27782 MsG0080048557.01.T01:intron 50.0%
CCCACGACAAAAGCTTGCCT+TGG - contig354end:27020-27039 None:intergenic 55.0%
GTTGCGCAACTTGCAACCCA+AGG + contig354end:27000-27019 MsG0080048557.01.T01:CDS 55.0%
TGAGTCGGCATTTCCAGAGC+TGG - contig354end:26482-26501 None:intergenic 55.0%
TGTCGTGGGGAGCAAAGGTA+AGG + contig354end:27031-27050 MsG0080048557.01.T01:intron 55.0%
! CCCAAGGCAAGCTTTTGTCG+TGG + contig354end:27016-27035 MsG0080048557.01.T01:CDS 55.0%
! CCCACTGTGGAGAGCTTTAG+TGG + contig354end:27330-27349 MsG0080048557.01.T01:intron 55.0%
! GCATTTCCAGAGCTGGCTTC+AGG - contig354end:26475-26494 None:intergenic 55.0%
! GCTTTTGTCGTGGGGAGCAA+AGG + contig354end:27026-27045 MsG0080048557.01.T01:CDS 55.0%
AAACTCGAGGCGGAGACGGT+GGG - contig354end:25496-25515 None:intergenic 60.0%
AGACACGGTGGCTGAAGTGG+AGG - contig354end:25473-25492 None:intergenic 60.0%
ATGCCTGCAGTAGGTGCGTC+AGG + contig354end:27509-27528 MsG0080048557.01.T01:intron 60.0%
GACACGGTGGCTGAAGTGGA+GGG - contig354end:25472-25491 None:intergenic 60.0%
GGAAGACCTGAAGCCAGCTC+TGG + contig354end:26466-26485 MsG0080048557.01.T01:five_prime_UTR 60.0%
GAAACTCGAGGCGGAGACGG+TGG - contig354end:25497-25516 None:intergenic 65.0%
GCTCCTGACGCACCTACTGC+AGG - contig354end:27515-27534 None:intergenic 65.0%
GGGAGACACGGTGGCTGAAG+TGG - contig354end:25476-25495 None:intergenic 65.0%
GTGGCTGAAGTGGAGGGACG+CGG - contig354end:25466-25485 None:intergenic 65.0%
GCTGAAGTGGAGGGACGCGG+CGG - contig354end:25463-25482 None:intergenic 70.0%
GGAGACGGTGGGAGACACGG+TGG - contig354end:25485-25504 None:intergenic 70.0%
GGCGGAGACGGTGGGAGACA+CGG - contig354end:25488-25507 None:intergenic 70.0%
GAAGTGGAGGGACGCGGCGG+TGG - contig354end:25460-25479 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
contig354end gene 25368 29406 25368 ID=MsG0080048557.01;Name=MsG0080048557.01
contig354end mRNA 25368 29406 25368 ID=MsG0080048557.01.T01;Parent=MsG0080048557.01;Name=MsG0080048557.01.T01;_AED=0.36;_eAED=0.36;_QI=342|0.85|1|1|0.71|0.5|8|303|165
contig354end exon 25368 25536 25368 ID=MsG0080048557.01.T01:exon:26460;Parent=MsG0080048557.01.T01
contig354end exon 26332 26569 26332 ID=MsG0080048557.01.T01:exon:26461;Parent=MsG0080048557.01.T01
contig354end exon 26993 27047 26993 ID=MsG0080048557.01.T01:exon:26462;Parent=MsG0080048557.01.T01
contig354end exon 27135 27215 27135 ID=MsG0080048557.01.T01:exon:26463;Parent=MsG0080048557.01.T01
contig354end exon 27770 27868 27770 ID=MsG0080048557.01.T01:exon:26464;Parent=MsG0080048557.01.T01
contig354end exon 28225 28266 28225 ID=MsG0080048557.01.T01:exon:26465;Parent=MsG0080048557.01.T01
contig354end exon 28921 29253 28921 ID=MsG0080048557.01.T01:exon:26466;Parent=MsG0080048557.01.T01
contig354end exon 29281 29406 29281 ID=MsG0080048557.01.T01:exon:26467;Parent=MsG0080048557.01.T01
contig354end five_prime_UTR 25368 25536 25368 ID=MsG0080048557.01.T01:five_prime_utr;Parent=MsG0080048557.01.T01
contig354end five_prime_UTR 26332 26504 26332 ID=MsG0080048557.01.T01:five_prime_utr;Parent=MsG0080048557.01.T01
contig354end CDS 26505 26569 26505 ID=MsG0080048557.01.T01:cds;Parent=MsG0080048557.01.T01
contig354end CDS 26993 27047 26993 ID=MsG0080048557.01.T01:cds;Parent=MsG0080048557.01.T01
contig354end CDS 27135 27215 27135 ID=MsG0080048557.01.T01:cds;Parent=MsG0080048557.01.T01
contig354end CDS 27770 27868 27770 ID=MsG0080048557.01.T01:cds;Parent=MsG0080048557.01.T01
contig354end CDS 28225 28266 28225 ID=MsG0080048557.01.T01:cds;Parent=MsG0080048557.01.T01
contig354end CDS 28921 29076 28921 ID=MsG0080048557.01.T01:cds;Parent=MsG0080048557.01.T01
contig354end three_prime_UTR 29077 29253 29077 ID=MsG0080048557.01.T01:three_prime_utr;Parent=MsG0080048557.01.T01
contig354end three_prime_UTR 29281 29406 29281 ID=MsG0080048557.01.T01:three_prime_utr;Parent=MsG0080048557.01.T01
Gene Sequence

>MsG0080048557.01.T01

ATGTCCAGAAAAAATCCATTTAATGGAAGGAAGTGGACAAATATTCTCCTTGCTGCCAATGTTTTATTTTATGTTGCGCAACTTGCAACCCAAGGCAAGCTTTTGTCGTGGGGAGCAAAGGTAAACAGTCTCATTGACAAAGGACAACTTTGGAGACTTGTTACATCTTCTTTTCTGCATGCAAATGTTGGACACCTAATGGTTGGATCGGTTGCGGTGTTTGTTTTAAGGCACAAAGACCTTGTAGGAGGTGGTAAAAAAGATCTGCAGCACATTGCACAAGTAATTGCCCTCAATATGCTTATTGGGTTATCATCAACTGGCATTGACAACTGGGGACATTTAGGAGGCTTGATTGGTGGAATTGCTGCATCATGGCTTATTGGACCTGCATGGAAGCATGAATCCACATCTTTAGATGGAAGAAGACTTTTCATTGACAGTGCACCAATATATAATCTCTTCAAGATTACGAGAGTACCTAAGCCATGGAAATAG
Protein sequence

>MsG0080048557.01.T01

MSRKNPFNGRKWTNILLAANVLFYVAQLATQGKLLSWGAKVNSLIDKGQLWRLVTSSFLHANVGHLMVGSVAVFVLRHKDLVGGGKKDLQHIAQVIALNMLIGLSSTGIDNWGHLGGLIGGIAASWLIGPAWKHESTSLDGRRLFIDSAPIYNLFKITRVPKPWK*