AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023674.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023674.01.T01 MTR_8g099345 76.712 219 13 1 1 219 1 181 3.71e-114 323
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023674.01.T01 AT1G64355 58.382 173 27 4 32 200 43 174 1.71e-57 181
MsG0480023674.01.T01 AT1G64355 69.792 96 22 3 32 123 43 135 1.12e-36 126
MsG0480023674.01.T01 AT1G64355 68.182 88 21 3 32 115 43 127 4.55e-31 111

Find 57 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGTAATTTATCATTAAT+TGG 0.227652 4:+88444203 MsG0480023674.01.T01:three_prime_UTR
TCGGATGTGCCTCCAAATTT+TGG 0.228216 4:+88444113 MsG0480023674.01.T01:three_prime_UTR
ACAGTTCCAGCTGGAATTTA+TGG 0.258898 4:+88443586 MsG0480023674.01.T01:intron
TGATCATTGCACATCAAAAT+TGG 0.269030 4:-88444236 None:intergenic
GGTGAGGTTAGGAGAAGTTT+TGG 0.272109 4:-88443424 None:intergenic
TTGAAGGGTTGATAAGTTTA+GGG 0.291880 4:-88443194 None:intergenic
GTTGAAGGGTTGATAAGTTT+AGG 0.306773 4:-88443195 None:intergenic
CACTAACTTGTTCATTGTGT+TGG 0.314311 4:+88443955 MsG0480023674.01.T01:CDS
TTAAATGAAAATCACGTTGC+AGG 0.357937 4:-88443224 None:intergenic
ACTAACTTGTTCATTGTGTT+GGG 0.374119 4:+88443956 MsG0480023674.01.T01:CDS
TTGACAGCTAAGGTGCATTA+TGG 0.386555 4:+88443875 MsG0480023674.01.T01:intron
TGCCCCTGGGTGTTGATCCA+TGG 0.395407 4:+88443310 MsG0480023674.01.T01:CDS
AATCAGAAAAGCTGGAAATT+CGG 0.416724 4:+88443991 MsG0480023674.01.T01:CDS
TCTCCTAACCTCACCCTCTT+CGG 0.418605 4:+88443432 MsG0480023674.01.T01:CDS
AAACCGAAGAGGGTGAGGTT+AGG 0.440423 4:-88443435 None:intergenic
GATCGTGTGTGGATTGCCCC+TGG 0.440978 4:+88443296 MsG0480023674.01.T01:CDS
TCTAATGTGGATTGGTTACA+TGG 0.443829 4:+88443908 MsG0480023674.01.T01:CDS
CTTCTTTGTCTAATGTGGAT+TGG 0.447286 4:+88443900 MsG0480023674.01.T01:CDS
ATGTGGATTGGTTACATGGT+GGG 0.450371 4:+88443912 MsG0480023674.01.T01:CDS
AACCGAACTGGACATTAAAC+CGG 0.454280 4:+88443669 MsG0480023674.01.T01:CDS
TGCCGGTTTAATGTCCAGTT+CGG 0.454901 4:-88443671 None:intergenic
TCGAAGGAGGAGAAGAAACC+TGG 0.458784 4:+88444028 MsG0480023674.01.T01:CDS
TGGAACTTCTTTGTCTAATG+TGG 0.464776 4:+88443895 MsG0480023674.01.T01:CDS
AGAGAGGGAATCAGAAAAGC+TGG 0.466045 4:+88443983 MsG0480023674.01.T01:CDS
GCCCCTGGGTGTTGATCCAT+GGG 0.473465 4:+88443311 MsG0480023674.01.T01:CDS
CGTTGCAGGAAGGTCGTTGA+AGG 0.474175 4:-88443210 None:intergenic
TGGAGGCACATCCGAGTCAA+TGG 0.486922 4:-88444105 None:intergenic
ATTGTGTTGGGTTTGAGAGA+GGG 0.496509 4:+88443968 MsG0480023674.01.T01:CDS
AGCGATGCTCTGTGAATCAA+TGG 0.506212 4:-88443340 None:intergenic
TGTAATTTGTTCCATTGACT+CGG 0.518063 4:+88444094 MsG0480023674.01.T01:three_prime_UTR
GGAGAAGAAACCTGGGATGA+AGG 0.519084 4:+88444036 MsG0480023674.01.T01:CDS
GACTCACCATAAATTCCAGC+TGG 0.525068 4:-88443592 None:intergenic
CATTGTGTTGGGTTTGAGAG+AGG 0.529587 4:+88443967 MsG0480023674.01.T01:CDS
GTGCCCATGGATCAACACCC+AGG 0.532974 4:-88443314 None:intergenic
AAGTACAAGAAACCAATGTA+AGG 0.534146 4:-88443390 None:intergenic
CAAGTTAGTGAGAGTGAGAA+GGG 0.535305 4:-88443942 None:intergenic
TGTGGATTGGTTACATGGTG+GGG 0.539691 4:+88443913 MsG0480023674.01.T01:CDS
AGTAGAAACCGAAGAGGGTG+AGG 0.547358 4:-88443440 None:intergenic
ATGCTGCTGTTGAACCATGC+CGG 0.548883 4:-88443688 None:intergenic
TGCCCATGGATCAACACCCA+GGG 0.560771 4:-88443313 None:intergenic
AGCAACTAGACCTTCATCCC+AGG 0.562545 4:-88444046 None:intergenic
AAATCACAGCCAAAATTTGG+AGG 0.572411 4:-88444122 None:intergenic
ATCGTGTGTGGATTGCCCCT+GGG 0.578639 4:+88443297 MsG0480023674.01.T01:CDS
GGAACAAATTACAATAACAC+TGG 0.586606 4:-88444084 None:intergenic
AATGTGGATTGGTTACATGG+TGG 0.588190 4:+88443911 MsG0480023674.01.T01:CDS
ATGAAAATCACGTTGCAGGA+AGG 0.588711 4:-88443220 None:intergenic
GATTTACAGAGAGATACAAC+AGG 0.591539 4:-88443171 None:intergenic
ACAAGTTAGTGAGAGTGAGA+AGG 0.592216 4:-88443943 None:intergenic
AACTGGACATTAAACCGGCA+TGG 0.599583 4:+88443674 MsG0480023674.01.T01:CDS
AAATTACAATAACACTGGCA+TGG 0.603957 4:-88444079 None:intergenic
TGAAGGGTTGATAAGTTTAG+GGG 0.614651 4:-88443193 None:intergenic
CGAAGGAGGAGAAGAAACCT+GGG 0.616213 4:+88444029 MsG0480023674.01.T01:CDS
GTTGCAGGAAGGTCGTTGAA+GGG 0.617837 4:-88443209 None:intergenic
AAGAGAACAGTGATCGTGTG+TGG 0.618841 4:+88443285 MsG0480023674.01.T01:CDS
GAATCAATGGAAGGTGCCCA+TGG 0.621299 4:-88443327 None:intergenic
GCCCATGGATCAACACCCAG+GGG 0.698475 4:-88443312 None:intergenic
ATGCTCTGTGAATCAATGGA+AGG 0.701730 4:-88443336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATAAATAAATAAAA+AGG - Chr4:88443563-88443582 None:intergenic 0.0%
!! ATAAATAAATAAAAAGGAAA+AGG - Chr4:88443557-88443576 None:intergenic 10.0%
!! TAATTTAATCATTTCAAATG+TGG - Chr4:88443835-88443854 None:intergenic 15.0%
!! TTGTGTAATTTATCATTAAT+TGG + Chr4:88444203-88444222 MsG0480023674.01.T01:three_prime_UTR 15.0%
!!! AAAAAAATTAATTTGCAGAA+TGG + Chr4:88443125-88443144 MsG0480023674.01.T01:exon 15.0%
!!! CTCTAATTGAACTTTATTTT+CGG - Chr4:88443629-88443648 None:intergenic 20.0%
! AAAAAATCACAGCCAAAATT+TGG - Chr4:88444128-88444147 None:intergenic 25.0%
! AAGAAAAGAAAACTAACTTG+TGG - Chr4:88443487-88443506 None:intergenic 25.0%
! ATTACTTATGTTTAGTTGTG+TGG + Chr4:88443851-88443870 MsG0480023674.01.T01:intron 25.0%
! TTGATTTATTATGATTCAGC+TGG + Chr4:88443764-88443783 MsG0480023674.01.T01:intron 25.0%
! TTTAATTGTTCTGTTTGGTT+CGG + Chr4:88443787-88443806 MsG0480023674.01.T01:intron 25.0%
!!! TTTTCTTTGTTTCCTTACAT+TGG + Chr4:88443378-88443397 MsG0480023674.01.T01:CDS 25.0%
AAATTACAATAACACTGGCA+TGG - Chr4:88444082-88444101 None:intergenic 30.0%
AAGTACAAGAAACCAATGTA+AGG - Chr4:88443393-88443412 None:intergenic 30.0%
AATCAGAAAAGCTGGAAATT+CGG + Chr4:88443991-88444010 MsG0480023674.01.T01:CDS 30.0%
CAACGATTAAGTTATTCGAA+CGG - Chr4:88443731-88443750 None:intergenic 30.0%
GGAACAAATTACAATAACAC+TGG - Chr4:88444087-88444106 None:intergenic 30.0%
TTAAATGAAAATCACGTTGC+AGG - Chr4:88443227-88443246 None:intergenic 30.0%
!! ACTAACTTGTTCATTGTGTT+GGG + Chr4:88443956-88443975 MsG0480023674.01.T01:CDS 30.0%
!! TGTAATTTGTTCCATTGACT+CGG + Chr4:88444094-88444113 MsG0480023674.01.T01:three_prime_UTR 30.0%
!! TTGAAGGGTTGATAAGTTTA+GGG - Chr4:88443197-88443216 None:intergenic 30.0%
!!! CAATGTTTTAAAAACCGAAC+TGG + Chr4:88443657-88443676 MsG0480023674.01.T01:CDS 30.0%
!!! GGTTCGGTTTTTAGAATATT+GGG + Chr4:88443803-88443822 MsG0480023674.01.T01:intron 30.0%
!!! TGGTTCGGTTTTTAGAATAT+TGG + Chr4:88443802-88443821 MsG0480023674.01.T01:intron 30.0%
AAATCACAGCCAAAATTTGG+AGG - Chr4:88444125-88444144 None:intergenic 35.0%
CAAAAAGTAGAAACCGAAGA+GGG - Chr4:88443448-88443467 None:intergenic 35.0%
CTTCTTTGTCTAATGTGGAT+TGG + Chr4:88443900-88443919 MsG0480023674.01.T01:CDS 35.0%
GATTTACAGAGAGATACAAC+AGG - Chr4:88443174-88443193 None:intergenic 35.0%
TGGAAATTCGGAGAAAAACA+CGG + Chr4:88444003-88444022 MsG0480023674.01.T01:CDS 35.0%
TGGAACTTCTTTGTCTAATG+TGG + Chr4:88443895-88443914 MsG0480023674.01.T01:CDS 35.0%
! GCTGGTTTAATTGTTCTGTT+TGG + Chr4:88443782-88443801 MsG0480023674.01.T01:intron 35.0%
!! CACTAACTTGTTCATTGTGT+TGG + Chr4:88443955-88443974 MsG0480023674.01.T01:CDS 35.0%
!! GTTGAAGGGTTGATAAGTTT+AGG - Chr4:88443198-88443217 None:intergenic 35.0%
!! TACTTTTTGCTCGCTTTTGT+TGG + Chr4:88443459-88443478 MsG0480023674.01.T01:CDS 35.0%
!! TCTAATGTGGATTGGTTACA+TGG + Chr4:88443908-88443927 MsG0480023674.01.T01:CDS 35.0%
!! TGAAGGGTTGATAAGTTTAG+GGG - Chr4:88443196-88443215 None:intergenic 35.0%
!!! GTTAGGAGAAGTTTTGGATT+TGG - Chr4:88443421-88443440 None:intergenic 35.0%
!!! TTATTTTGAACAGTTCCAGC+TGG + Chr4:88443577-88443596 MsG0480023674.01.T01:intron 35.0%
AACCGAACTGGACATTAAAC+CGG + Chr4:88443669-88443688 MsG0480023674.01.T01:CDS 40.0%
ACAAGTTAGTGAGAGTGAGA+AGG - Chr4:88443946-88443965 None:intergenic 40.0%
ACAGTTCCAGCTGGAATTTA+TGG + Chr4:88443586-88443605 MsG0480023674.01.T01:intron 40.0%
ATGAAAATCACGTTGCAGGA+AGG - Chr4:88443223-88443242 None:intergenic 40.0%
ATGCTCTGTGAATCAATGGA+AGG - Chr4:88443339-88443358 None:intergenic 40.0%
ATTGTGTTGGGTTTGAGAGA+GGG + Chr4:88443968-88443987 MsG0480023674.01.T01:CDS 40.0%
CAAGTTAGTGAGAGTGAGAA+GGG - Chr4:88443945-88443964 None:intergenic 40.0%
GCAAAAAGTAGAAACCGAAG+AGG - Chr4:88443449-88443468 None:intergenic 40.0%
TTGACAGCTAAGGTGCATTA+TGG + Chr4:88443875-88443894 MsG0480023674.01.T01:intron 40.0%
!! AATGTGGATTGGTTACATGG+TGG + Chr4:88443911-88443930 MsG0480023674.01.T01:CDS 40.0%
!! ATGTGGATTGGTTACATGGT+GGG + Chr4:88443912-88443931 MsG0480023674.01.T01:CDS 40.0%
AACTGGACATTAAACCGGCA+TGG + Chr4:88443674-88443693 MsG0480023674.01.T01:CDS 45.0%
AAGAGAACAGTGATCGTGTG+TGG + Chr4:88443285-88443304 MsG0480023674.01.T01:CDS 45.0%
AGAGAGGGAATCAGAAAAGC+TGG + Chr4:88443983-88444002 MsG0480023674.01.T01:CDS 45.0%
AGCGATGCTCTGTGAATCAA+TGG - Chr4:88443343-88443362 None:intergenic 45.0%
CATTGTGTTGGGTTTGAGAG+AGG + Chr4:88443967-88443986 MsG0480023674.01.T01:CDS 45.0%
GAAAAACACGGAATCGAAGG+AGG + Chr4:88444015-88444034 MsG0480023674.01.T01:CDS 45.0%
GACTCACCATAAATTCCAGC+TGG - Chr4:88443595-88443614 None:intergenic 45.0%
GCAGCATACAGAATGACAGT+TGG + Chr4:88443704-88443723 MsG0480023674.01.T01:intron 45.0%
GGAGAAAAACACGGAATCGA+AGG + Chr4:88444012-88444031 MsG0480023674.01.T01:CDS 45.0%
! GGTGAGGTTAGGAGAAGTTT+TGG - Chr4:88443427-88443446 None:intergenic 45.0%
! TCGGATGTGCCTCCAAATTT+TGG + Chr4:88444113-88444132 MsG0480023674.01.T01:three_prime_UTR 45.0%
!! TGCCGGTTTAATGTCCAGTT+CGG - Chr4:88443674-88443693 None:intergenic 45.0%
!! TGTGGATTGGTTACATGGTG+GGG + Chr4:88443913-88443932 MsG0480023674.01.T01:CDS 45.0%
AAACCGAAGAGGGTGAGGTT+AGG - Chr4:88443438-88443457 None:intergenic 50.0%
AGCAACTAGACCTTCATCCC+AGG - Chr4:88444049-88444068 None:intergenic 50.0%
AGTAGAAACCGAAGAGGGTG+AGG - Chr4:88443443-88443462 None:intergenic 50.0%
ATGCTGCTGTTGAACCATGC+CGG - Chr4:88443691-88443710 None:intergenic 50.0%
CGAAGGAGGAGAAGAAACCT+GGG + Chr4:88444029-88444048 MsG0480023674.01.T01:CDS 50.0%
GGAGAAGAAACCTGGGATGA+AGG + Chr4:88444036-88444055 MsG0480023674.01.T01:CDS 50.0%
GTTGCAGGAAGGTCGTTGAA+GGG - Chr4:88443212-88443231 None:intergenic 50.0%
GTTGTGTGGCTTGACAGCTA+AGG + Chr4:88443865-88443884 MsG0480023674.01.T01:intron 50.0%
TCGAAGGAGGAGAAGAAACC+TGG + Chr4:88444028-88444047 MsG0480023674.01.T01:CDS 50.0%
TCTCCTAACCTCACCCTCTT+CGG + Chr4:88443432-88443451 MsG0480023674.01.T01:CDS 50.0%
!! GAATCAATGGAAGGTGCCCA+TGG - Chr4:88443330-88443349 None:intergenic 50.0%
CGTTGCAGGAAGGTCGTTGA+AGG - Chr4:88443213-88443232 None:intergenic 55.0%
TGCCCATGGATCAACACCCA+GGG - Chr4:88443316-88443335 None:intergenic 55.0%
! ATCGTGTGTGGATTGCCCCT+GGG + Chr4:88443297-88443316 MsG0480023674.01.T01:CDS 55.0%
! TGGAGGCACATCCGAGTCAA+TGG - Chr4:88444108-88444127 None:intergenic 55.0%
GCCCATGGATCAACACCCAG+GGG - Chr4:88443315-88443334 None:intergenic 60.0%
GTGCCCATGGATCAACACCC+AGG - Chr4:88443317-88443336 None:intergenic 60.0%
! GATCGTGTGTGGATTGCCCC+TGG + Chr4:88443296-88443315 MsG0480023674.01.T01:CDS 60.0%
!! GCCCCTGGGTGTTGATCCAT+GGG + Chr4:88443311-88443330 MsG0480023674.01.T01:CDS 60.0%
!! TGCCCCTGGGTGTTGATCCA+TGG + Chr4:88443310-88443329 MsG0480023674.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 88443118 88444249 88443118 ID=MsG0480023674.01;Name=MsG0480023674.01
Chr4 mRNA 88443118 88444249 88443118 ID=MsG0480023674.01.T01;Parent=MsG0480023674.01;Name=MsG0480023674.01.T01;_AED=0.50;_eAED=0.56;_QI=26|0.5|0.66|1|1|1|3|186|219
Chr4 exon 88443118 88443489 88443118 ID=MsG0480023674.01.T01:exon:12359;Parent=MsG0480023674.01.T01
Chr4 exon 88443590 88443721 88443590 ID=MsG0480023674.01.T01:exon:12360;Parent=MsG0480023674.01.T01
Chr4 exon 88443882 88444249 88443882 ID=MsG0480023674.01.T01:exon:12361;Parent=MsG0480023674.01.T01
Chr4 five_prime_UTR 88443118 88443143 88443118 ID=MsG0480023674.01.T01:five_prime_utr;Parent=MsG0480023674.01.T01
Chr4 CDS 88443144 88443489 88443144 ID=MsG0480023674.01.T01:cds;Parent=MsG0480023674.01.T01
Chr4 CDS 88443590 88443721 88443590 ID=MsG0480023674.01.T01:cds;Parent=MsG0480023674.01.T01
Chr4 CDS 88443882 88444063 88443882 ID=MsG0480023674.01.T01:cds;Parent=MsG0480023674.01.T01
Chr4 three_prime_UTR 88444064 88444249 88444064 ID=MsG0480023674.01.T01:three_prime_utr;Parent=MsG0480023674.01.T01
Gene Sequence

>MsG0480023674.01.T01

ATGGCTTCTTCTGCTATAAACTTATTACCTGTTGTATCTCTCTGTAAATCCCCTAAACTTATCAACCCTTCAACGACCTTCCTGCAACGTGATTTTCATTTAAGCAGCGTCAGAACAATCAGAGAATCAAACAGCTTTAGAAAGAGAACAGTGATCGTGTGTGGATTGCCCCTGGGTGTTGATCCATGGGCACCTTCCATTGATTCACAGAGCATCGCTTCTCAACTATTTGCGTTTTCTTTGTTTCCTTACATTGGTTTCTTGTACTTCATTACCAAATCCAAAACTTCTCCTAACCTCACCCTCTTCGGTTTCTACTTTTTGCTCGCTTTTGTTGGTGCCACAATTCCAGCTGGAATTTATGGTGAGTCAAGTTTATTTGCCGAAAATAAAGTTCAATTAGAGATGCATATCAATGTTTTAAAAACCGAACTGGACATTAAACCGGCATGGTTCAACAGCAGCATACAGAATGACACTAAGGTGCATTATGGAACTTCTTTGTCTAATGTGGATTGGTTACATGGTGGGGCTGAGTCCCTTCTCACTCTCACTAACTTGTTCATTGTGTTGGGTTTGAGAGAGGGAATCAGAAAAGCTGGAAATTCGGAGAAAAACACGGAATCGAAGGAGGAGAAGAAACCTGGGATGAAGGTCTAG

Protein sequence

>MsG0480023674.01.T01

MASSAINLLPVVSLCKSPKLINPSTTFLQRDFHLSSVRTIRESNSFRKRTVIVCGLPLGVDPWAPSIDSQSIASQLFAFSLFPYIGFLYFITKSKTSPNLTLFGFYFLLAFVGATIPAGIYGESSLFAENKVQLEMHINVLKTELDIKPAWFNSSIQNDTKVHYGTSLSNVDWLHGGAESLLTLTNLFIVLGLREGIRKAGNSEKNTESKEEKKPGMKV*