Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049051.01.T01 | KEH35470.1 | 97.101 | 69 | 2 | 0 | 1 | 69 | 16 | 84 | 2.05E-39 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049051.01.T01 | A0A072VBC0 | 97.101 | 69 | 2 | 0 | 1 | 69 | 16 | 84 | 9.79e-40 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048197.01 | MsG0080049051.01 | 0.803120 | 4.056962e-49 | 2.022051e-46 |
| MsG0080049051.01 | MsG0080049052.01 | 0.998131 | 6.705665e-257 | 1.823478e-249 |
| MsG0080049051.01 | MsG0080049059.01 | 0.802647 | 5.079873e-49 | 2.501603e-46 |
| MsG0080049051.01 | MsG0280006671.01 | -0.812537 | 4.032677e-51 | 2.570890e-48 |
| MsG0080049051.01 | MsG0280007607.01 | 0.803920 | 2.768585e-49 | 1.408708e-46 |
| MsG0080049051.01 | MsG0280007715.01 | 0.820281 | 7.453716e-53 | 5.872039e-50 |
| MsG0080049051.01 | MsG0280011457.01 | 0.810442 | 1.150560e-50 | 6.935915e-48 |
| MsG0080049051.01 | MsG0380014137.01 | -0.800948 | 1.134251e-48 | 5.350347e-46 |
| MsG0080049051.01 | MsG0480018127.01 | -0.833690 | 4.613936e-56 | 5.353409e-53 |
| MsG0080049051.01 | MsG0480021493.01 | -0.802870 | 4.569688e-49 | 2.263228e-46 |
| MsG0080049051.01 | MsG0480023726.01 | 0.855297 | 6.933276e-62 | 1.593334e-58 |
| MsG0080049051.01 | MsG0580029635.01 | 0.816350 | 5.787210e-52 | 4.091000e-49 |
| MsG0080049051.01 | MsG0580030095.01 | -0.849088 | 4.046584e-60 | 7.585183e-57 |
| MsG0080049051.01 | MsG0680034751.01 | 0.800894 | 1.163515e-48 | 5.480895e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049051.01.T01 | MTR_3g491900 | 97.101 | 69 | 2 | 0 | 1 | 69 | 16 | 84 | 2.48e-43 | 134 |
| MsG0080049051.01.T01 | MTR_8g027380 | 72.414 | 29 | 8 | 0 | 1 | 29 | 21 | 49 | 8.49e-11 | 52.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049051.01.T01 | AT4G10810 | 68.750 | 32 | 10 | 0 | 2 | 33 | 29 | 60 | 5.06e-11 | 53.1 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGGCTTTCTTTGCATCTT+TGG | 0.266381 | contig637end:-7464 | MsG0080049051.01.T01:CDS |
| GGAGGAGGAGGCTGTGGTTG+AGG | 0.362552 | contig637end:+7340 | None:intergenic |
| AGGATGAGAAGAGGATCGTC+AGG | 0.481227 | contig637end:+7403 | None:intergenic |
| TCGGAAGGAGGAGGAGGCTG+TGG | 0.506854 | contig637end:+7334 | None:intergenic |
| CTTTCTTTGCATCTTTGGAA+CGG | 0.508553 | contig637end:-7459 | MsG0080049051.01.T01:CDS |
| ACGGAATGGCGAGCTGGATA+GGG | 0.508733 | contig637end:-7498 | MsG0080049052.01.T01:CDS |
| AACGGAATGGCGAGCTGGAT+AGG | 0.517911 | contig637end:-7499 | MsG0080049052.01.T01:CDS |
| AAGAGGATCGTCAGGATCAT+CGG | 0.521870 | contig637end:+7411 | None:intergenic |
| TTGTTGTCGGAAGGAGGAGG+AGG | 0.522839 | contig637end:+7328 | None:intergenic |
| ATCGTCAGGATCATCGGAGG+TGG | 0.525533 | contig637end:+7417 | None:intergenic |
| AGATTCATCGGAGAATCTGT+TGG | 0.545815 | contig637end:+7369 | None:intergenic |
| GGAGAATCTGTTGGAATCAG+AGG | 0.559994 | contig637end:+7378 | None:intergenic |
| GCTGGAACGGAATGGCGAGC+TGG | 0.561719 | contig637end:-7504 | MsG0080049052.01.T01:CDS |
| ATCTGTTGGAATCAGAGGGA+AGG | 0.586267 | contig637end:+7383 | None:intergenic |
| TTGAGGATGAGAAGATTCAT+CGG | 0.597564 | contig637end:+7357 | None:intergenic |
| TCAGAGGGAAGGATGAGAAG+AGG | 0.603416 | contig637end:+7394 | None:intergenic |
| GAGAATCTGTTGGAATCAGA+GGG | 0.616835 | contig637end:+7379 | None:intergenic |
| GATAGGGATGAATCTTGCAA+CGG | 0.636350 | contig637end:-7482 | MsG0080049051.01.T01:CDS |
| CTACCCTAAAACTCAAACAG+CGG | 0.639714 | contig637end:+7294 | None:intergenic |
| AGGATCGTCAGGATCATCGG+AGG | 0.716513 | contig637end:+7414 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGAGGATGAGAAGATTCAT+CGG | + | contig637end:7445-7464 | None:intergenic | 35.0% | |
| ! | CTTTCTTTGCATCTTTGGAA+CGG | - | contig637end:7340-7359 | MsG0080049051.01.T01:CDS | 35.0% |
| !!! | TTGTTGTTTTTGTTGTCGGA+AGG | + | contig637end:7483-7502 | None:intergenic | 35.0% |
| AACGGCTTTCTTTGCATCTT+TGG | - | contig637end:7335-7354 | MsG0080049051.01.T01:CDS | 40.0% | |
| AGATTCATCGGAGAATCTGT+TGG | + | contig637end:7433-7452 | None:intergenic | 40.0% | |
| GAGAATCTGTTGGAATCAGA+GGG | + | contig637end:7423-7442 | None:intergenic | 40.0% | |
| GATAGGGATGAATCTTGCAA+CGG | - | contig637end:7317-7336 | MsG0080049051.01.T01:CDS | 40.0% | |
| !!! | GGCGTTGTTGTTTTTGTTGT+CGG | + | contig637end:7487-7506 | None:intergenic | 40.0% |
| !!! | TTGTTTTTGTTGTCGGAAGG+AGG | + | contig637end:7480-7499 | None:intergenic | 40.0% |
| AAGAGGATCGTCAGGATCAT+CGG | + | contig637end:7391-7410 | None:intergenic | 45.0% | |
| ATCTGTTGGAATCAGAGGGA+AGG | + | contig637end:7419-7438 | None:intergenic | 45.0% | |
| GGAGAATCTGTTGGAATCAG+AGG | + | contig637end:7424-7443 | None:intergenic | 45.0% | |
| !! | TTTTTGTTGTCGGAAGGAGG+AGG | + | contig637end:7477-7496 | None:intergenic | 45.0% |
| AGGATGAGAAGAGGATCGTC+AGG | + | contig637end:7399-7418 | None:intergenic | 50.0% | |
| TCAGAGGGAAGGATGAGAAG+AGG | + | contig637end:7408-7427 | None:intergenic | 50.0% | |
| AGGATCGTCAGGATCATCGG+AGG | + | contig637end:7388-7407 | None:intergenic | 55.0% | |
| ATCGTCAGGATCATCGGAGG+TGG | + | contig637end:7385-7404 | None:intergenic | 55.0% | |
| TTGTTGTCGGAAGGAGGAGG+AGG | + | contig637end:7474-7493 | None:intergenic | 55.0% | |
| GGAGGAGGAGGCTGTGGTTG+AGG | + | contig637end:7462-7481 | None:intergenic | 65.0% | |
| TCGGAAGGAGGAGGAGGCTG+TGG | + | contig637end:7468-7487 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig637end | gene | 7306 | 7515 | 7306 | ID=MsG0080049051.01;Name=MsG0080049051.01 |
| contig637end | mRNA | 7306 | 7515 | 7306 | ID=MsG0080049051.01.T01;Parent=MsG0080049051.01;Name=MsG0080049051.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|69 |
| contig637end | exon | 7306 | 7515 | 7306 | ID=MsG0080049051.01.T01:exon:14478;Parent=MsG0080049051.01.T01 |
| contig637end | CDS | 7306 | 7515 | 7306 | ID=MsG0080049051.01.T01:cds;Parent=MsG0080049051.01.T01 |
| Gene Sequence |
| Protein sequence |
