AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007715.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007715.01.T01 MTR_2g030450 98.675 151 1 1 1 151 464 613 2.74e-101 303
MsG0280007715.01.T01 MTR_2g005905 77.273 66 4 3 48 111 119 175 1.51e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007715.01.T01 AT1G77180 69.281 153 42 4 1 149 458 609 1.82e-62 202
MsG0280007715.01.T01 AT1G77180 69.281 153 42 4 1 149 458 609 1.82e-62 202
MsG0280007715.01.T01 AT1G77180 58.182 55 21 1 97 149 453 507 4.16e-12 63.2

Find 49 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCCTGACAAATCATTTAC+AGG 0.274740 2:-19536442 MsG0280007715.01.T01:CDS
CAGGATAAAGGAATGTCTTC+TGG 0.319470 2:-19536625 MsG0280007715.01.T01:CDS
CAGTATAATGTGTATGATAA+AGG 0.369891 2:-19536586 MsG0280007715.01.T01:CDS
CATCCTTCTTCGGCCTGTAC+AGG 0.384677 2:+19536533 None:intergenic
AGGCTATTCAACCAGGATAA+AGG 0.404186 2:-19536637 MsG0280007715.01.T01:CDS
TCATTATCAACATCCTTCTT+CGG 0.413809 2:+19536523 None:intergenic
GATAGTTCTGATGGAGGTTC+CGG 0.422860 2:-19536250 MsG0280007715.01.T01:CDS
CAGAAGACATTCCTTTATCC+TGG 0.436234 2:+19536626 None:intergenic
GATGTTGATAATGAAGCTTA+TGG 0.443186 2:-19536514 MsG0280007715.01.T01:CDS
AGGAGATGCAGACGAGCAGC+TGG 0.446435 2:-19536491 MsG0280007715.01.T01:CDS
GTCCCTGTAAATGATTTGTC+AGG 0.453898 2:+19536439 None:intergenic
CTTCTGAAAGGGCACCTCCT+AGG 0.457735 2:-19536417 MsG0280007715.01.T01:CDS
AGCGAAGAAGCTGATCCATT+TGG 0.463504 2:-19536373 MsG0280007715.01.T01:CDS
TTCTGAAAGGGCACCTCCTA+GGG 0.483106 2:-19536416 MsG0280007715.01.T01:CDS
ACTATCTCGCATTGAAGATC+CGG 0.487858 2:+19536267 None:intergenic
AAGAACTGATCAAGACCAAA+TGG 0.488924 2:+19536358 None:intergenic
AAATCCAATGCGAGTCCTGC+CGG 0.503052 2:+19536231 None:intergenic
AGAGGTCAAGAAGGGTAAGA+AGG 0.519440 2:-19536332 MsG0280007715.01.T01:CDS
ATGGAAAATGTAGGTGGTGG+AGG 0.522102 2:-19536307 MsG0280007715.01.T01:CDS
CACCCTGTACAGGCCGAAGA+AGG 0.525352 2:-19536536 MsG0280007715.01.T01:CDS
GGCACCTCCTAGGGATAGGC+CGG 0.527286 2:-19536407 MsG0280007715.01.T01:CDS
TCTTCGCTCTCAAACTCAAC+CGG 0.528979 2:+19536388 None:intergenic
CAACACTTTCCACCCTGTAC+AGG 0.532515 2:-19536546 MsG0280007715.01.T01:CDS
ACTCGCATTGGATTTGAAAG+AGG 0.533878 2:-19536223 MsG0280007715.01.T01:CDS
ATTTACAGGGACTTCTGAAA+GGG 0.539211 2:-19536428 MsG0280007715.01.T01:CDS
CATTTACAGGGACTTCTGAA+AGG 0.540154 2:-19536429 MsG0280007715.01.T01:CDS
AGCCTGACAAATCATTTACA+GGG 0.540192 2:-19536441 MsG0280007715.01.T01:CDS
ATCCTTCTTCGGCCTGTACA+GGG 0.543020 2:+19536534 None:intergenic
GGTTCCGGCAGGACTCGCAT+TGG 0.545962 2:-19536235 MsG0280007715.01.T01:CDS
AAAGGGCACCTCCTAGGGAT+AGG 0.554982 2:-19536411 MsG0280007715.01.T01:CDS
GCCATGGAAAATGTAGGTGG+TGG 0.556473 2:-19536310 MsG0280007715.01.T01:CDS
TCAATGCGAGATAGTTCTGA+TGG 0.566163 2:-19536259 MsG0280007715.01.T01:CDS
GTTCTTGACAGAGGTCAAGA+AGG 0.569172 2:-19536341 MsG0280007715.01.T01:CDS
GTTCTGATGGAGGTTCCGGC+AGG 0.578090 2:-19536246 MsG0280007715.01.T01:CDS
AACTCAACCGGCCTATCCCT+AGG 0.579497 2:+19536400 None:intergenic
CAAGAAGGGTAAGAAGGCCA+TGG 0.599861 2:-19536326 MsG0280007715.01.T01:CDS
AAGAAGGCCATGGAAAATGT+AGG 0.600100 2:-19536316 MsG0280007715.01.T01:CDS
GTTGATAATGAAGCTTATGG+AGG 0.605802 2:-19536511 MsG0280007715.01.T01:CDS
TTCTTGACAGAGGTCAAGAA+GGG 0.628622 2:-19536340 MsG0280007715.01.T01:CDS
TGACGAGAGGCTATTCAACC+AGG 0.632689 2:-19536644 MsG0280007715.01.T01:CDS
GGAACTATGAGAGCTAGTGC+CGG 0.638687 2:-19536286 MsG0280007715.01.T01:CDS
TCTTGATCAGTTCTTGACAG+AGG 0.641422 2:-19536350 MsG0280007715.01.T01:CDS
CACATTATACTGATCATCAG+TGG 0.646545 2:+19536597 None:intergenic
CTGTACAGGGTGGAAAGTGT+TGG 0.650930 2:+19536547 None:intergenic
ATGCGAGATAGTTCTGATGG+AGG 0.651636 2:-19536256 MsG0280007715.01.T01:CDS
CTTCTTCGGCCTGTACAGGG+TGG 0.668533 2:+19536537 None:intergenic
CAGAGGTCATGTATGACGAG+AGG 0.675837 2:-19536657 None:intergenic
TCAACCGGCCTATCCCTAGG+AGG 0.688076 2:+19536403 None:intergenic
AAGGCCATGGAAAATGTAGG+TGG 0.706994 2:-19536313 MsG0280007715.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAGTATAATGTGTATGATAA+AGG - Chr2:19536279-19536298 MsG0280007715.01.T01:CDS 25.0%
TCATTATCAACATCCTTCTT+CGG + Chr2:19536345-19536364 None:intergenic 30.0%
!! GATGTTGATAATGAAGCTTA+TGG - Chr2:19536351-19536370 MsG0280007715.01.T01:CDS 30.0%
AAGAACTGATCAAGACCAAA+TGG + Chr2:19536510-19536529 None:intergenic 35.0%
AAGCCTGACAAATCATTTAC+AGG - Chr2:19536423-19536442 MsG0280007715.01.T01:CDS 35.0%
AGCCTGACAAATCATTTACA+GGG - Chr2:19536424-19536443 MsG0280007715.01.T01:CDS 35.0%
CACATTATACTGATCATCAG+TGG + Chr2:19536271-19536290 None:intergenic 35.0%
! ATTTACAGGGACTTCTGAAA+GGG - Chr2:19536437-19536456 MsG0280007715.01.T01:CDS 35.0%
!! GTTGATAATGAAGCTTATGG+AGG - Chr2:19536354-19536373 MsG0280007715.01.T01:CDS 35.0%
AAGAAGGCCATGGAAAATGT+AGG - Chr2:19536549-19536568 MsG0280007715.01.T01:CDS 40.0%
ACTATCTCGCATTGAAGATC+CGG + Chr2:19536601-19536620 None:intergenic 40.0%
ACTCGCATTGGATTTGAAAG+AGG - Chr2:19536642-19536661 MsG0280007715.01.T01:CDS 40.0%
AGGCTATTCAACCAGGATAA+AGG - Chr2:19536228-19536247 MsG0280007715.01.T01:CDS 40.0%
CAGAAGACATTCCTTTATCC+TGG + Chr2:19536242-19536261 None:intergenic 40.0%
CAGGATAAAGGAATGTCTTC+TGG - Chr2:19536240-19536259 MsG0280007715.01.T01:CDS 40.0%
CATTTACAGGGACTTCTGAA+AGG - Chr2:19536436-19536455 MsG0280007715.01.T01:CDS 40.0%
TCTTGATCAGTTCTTGACAG+AGG - Chr2:19536515-19536534 MsG0280007715.01.T01:CDS 40.0%
TTCTTGACAGAGGTCAAGAA+GGG - Chr2:19536525-19536544 MsG0280007715.01.T01:CDS 40.0%
! GTCCCTGTAAATGATTTGTC+AGG + Chr2:19536429-19536448 None:intergenic 40.0%
!! TCAATGCGAGATAGTTCTGA+TGG - Chr2:19536606-19536625 MsG0280007715.01.T01:CDS 40.0%
AAGGCCATGGAAAATGTAGG+TGG - Chr2:19536552-19536571 MsG0280007715.01.T01:CDS 45.0%
AGAGGTCAAGAAGGGTAAGA+AGG - Chr2:19536533-19536552 MsG0280007715.01.T01:CDS 45.0%
AGCGAAGAAGCTGATCCATT+TGG - Chr2:19536492-19536511 MsG0280007715.01.T01:CDS 45.0%
GTTCTTGACAGAGGTCAAGA+AGG - Chr2:19536524-19536543 MsG0280007715.01.T01:CDS 45.0%
TCTTCGCTCTCAAACTCAAC+CGG + Chr2:19536480-19536499 None:intergenic 45.0%
! ATGGAAAATGTAGGTGGTGG+AGG - Chr2:19536558-19536577 MsG0280007715.01.T01:CDS 45.0%
! TCCACCACCTACATTTTCCA+TGG + Chr2:19536559-19536578 None:intergenic 45.0%
!! ATGCGAGATAGTTCTGATGG+AGG - Chr2:19536609-19536628 MsG0280007715.01.T01:CDS 45.0%
!! GATAGTTCTGATGGAGGTTC+CGG - Chr2:19536615-19536634 MsG0280007715.01.T01:CDS 45.0%
AAATCCAATGCGAGTCCTGC+CGG + Chr2:19536637-19536656 None:intergenic 50.0%
ATCCTTCTTCGGCCTGTACA+GGG + Chr2:19536334-19536353 None:intergenic 50.0%
CAACACTTTCCACCCTGTAC+AGG - Chr2:19536319-19536338 MsG0280007715.01.T01:CDS 50.0%
CAAGAAGGGTAAGAAGGCCA+TGG - Chr2:19536539-19536558 MsG0280007715.01.T01:CDS 50.0%
GCCATGGAAAATGTAGGTGG+TGG - Chr2:19536555-19536574 MsG0280007715.01.T01:CDS 50.0%
GGAACTATGAGAGCTAGTGC+CGG - Chr2:19536579-19536598 MsG0280007715.01.T01:CDS 50.0%
TGACGAGAGGCTATTCAACC+AGG - Chr2:19536221-19536240 MsG0280007715.01.T01:CDS 50.0%
! CTGTACAGGGTGGAAAGTGT+TGG + Chr2:19536321-19536340 None:intergenic 50.0%
!! TTCTGAAAGGGCACCTCCTA+GGG - Chr2:19536449-19536468 MsG0280007715.01.T01:CDS 50.0%
AACTCAACCGGCCTATCCCT+AGG + Chr2:19536468-19536487 None:intergenic 55.0%
CATCCTTCTTCGGCCTGTAC+AGG + Chr2:19536335-19536354 None:intergenic 55.0%
!! AAAGGGCACCTCCTAGGGAT+AGG - Chr2:19536454-19536473 MsG0280007715.01.T01:CDS 55.0%
!! CTTCTGAAAGGGCACCTCCT+AGG - Chr2:19536448-19536467 MsG0280007715.01.T01:CDS 55.0%
AGGAGATGCAGACGAGCAGC+TGG - Chr2:19536374-19536393 MsG0280007715.01.T01:CDS 60.0%
CACCCTGTACAGGCCGAAGA+AGG - Chr2:19536329-19536348 MsG0280007715.01.T01:CDS 60.0%
CTTCTTCGGCCTGTACAGGG+TGG + Chr2:19536331-19536350 None:intergenic 60.0%
GTTCTGATGGAGGTTCCGGC+AGG - Chr2:19536619-19536638 MsG0280007715.01.T01:CDS 60.0%
TCAACCGGCCTATCCCTAGG+AGG + Chr2:19536465-19536484 None:intergenic 60.0%
GGCACCTCCTAGGGATAGGC+CGG - Chr2:19536458-19536477 MsG0280007715.01.T01:CDS 65.0%
GGTTCCGGCAGGACTCGCAT+TGG - Chr2:19536630-19536649 MsG0280007715.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 19536216 19536671 19536216 ID=MsG0280007715.01;Name=MsG0280007715.01
Chr2 mRNA 19536216 19536671 19536216 ID=MsG0280007715.01.T01;Parent=MsG0280007715.01;Name=MsG0280007715.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|151
Chr2 exon 19536216 19536671 19536216 ID=MsG0280007715.01.T01:exon:2253;Parent=MsG0280007715.01.T01
Chr2 CDS 19536216 19536671 19536216 ID=MsG0280007715.01.T01:cds;Parent=MsG0280007715.01.T01
Gene Sequence

>MsG0280007715.01.T01

ATGTATGACGAGAGGCTATTCAACCAGGATAAAGGAATGTCTTCTGGATTTGCCACTGATGATCAGTATAATGTGTATGATAAAGGCTTGTTCAATGCACAGCCAACACTTTCCACCCTGTACAGGCCGAAGAAGGATGTTGATAATGAAGCTTATGGAGGAGATGCAGACGAGCAGCTGGAGAAGATTACGAAGACTGATCGCTTTAAGCCTGACAAATCATTTACAGGGACTTCTGAAAGGGCACCTCCTAGGGATAGGCCGGTTGAGTTTGAGAGCGAAGAAGCTGATCCATTTGGTCTTGATCAGTTCTTGACAGAGGTCAAGAAGGGTAAGAAGGCCATGGAAAATGTAGGTGGTGGAGGAACTATGAGAGCTAGTGCCGGATCTTCAATGCGAGATAGTTCTGATGGAGGTTCCGGCAGGACTCGCATTGGATTTGAAAGAGGTCGTTAA

Protein sequence

>MsG0280007715.01.T01

MYDERLFNQDKGMSSGFATDDQYNVYDKGLFNAQPTLSTLYRPKKDVDNEAYGGDADEQLEKITKTDRFKPDKSFTGTSERAPPRDRPVEFESEEADPFGLDQFLTEVKKGKKAMENVGGGGTMRASAGSSMRDSSDGGSGRTRIGFERGR*