AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000009.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000009.01.T01 MTR_1g026310 89.464 560 22 2 1 523 1 560 0.0 995
MsG0180000009.01.T01 MTR_8g039720 75.220 569 85 8 1 523 1 559 0.0 827
MsG0180000009.01.T01 MTR_1g026470 86.386 404 18 2 1 367 1 404 0.0 677
MsG0180000009.01.T01 MTR_1g026430 87.032 401 15 2 1 364 1 401 0.0 677
MsG0180000009.01.T01 MTR_4g057730 43.094 543 270 6 17 523 3 542 2.79e-146 431
MsG0180000009.01.T01 MTR_5g027480 42.435 542 272 6 18 523 18 555 4.02e-145 429
MsG0180000009.01.T01 MTR_1g090737 44.118 544 256 8 15 516 22 559 3.58e-144 427
MsG0180000009.01.T01 MTR_2g095510 41.360 544 275 7 17 523 3 539 5.03e-140 415
MsG0180000009.01.T01 MTR_3g088870 41.727 556 257 12 14 519 16 554 3.88e-139 413
MsG0180000009.01.T01 MTR_5g027500 42.210 552 255 8 26 523 27 568 1.57e-137 409
MsG0180000009.01.T01 MTR_3g088880 41.250 560 265 12 7 519 10 552 6.71e-137 407
MsG0180000009.01.T01 MTR_4g077900 43.451 481 232 5 80 523 1 478 2.11e-136 404
MsG0180000009.01.T01 MTR_1g063110 41.405 541 271 8 27 523 13 551 2.97e-131 393
MsG0180000009.01.T01 MTR_1g063120 41.101 545 271 8 27 523 13 555 2.70e-129 388
MsG0180000009.01.T01 MTR_1g073180 40.110 546 265 12 14 516 1 527 1.91e-118 360
MsG0180000009.01.T01 MTR_4g057730 41.102 472 239 6 17 452 3 471 3.51e-114 346
MsG0180000009.01.T01 MTR_1g073180 38.764 534 262 13 8 498 20 531 7.21e-105 324
MsG0180000009.01.T01 MTR_2g105570 37.291 539 280 9 26 515 12 541 2.42e-104 323
MsG0180000009.01.T01 MTR_3g460770 33.396 536 303 6 20 513 8 531 4.02e-104 323
MsG0180000009.01.T01 MTR_4g128337 37.072 526 293 4 26 515 17 540 5.54e-104 323
MsG0180000009.01.T01 MTR_4g057730 40.000 450 231 6 17 430 3 449 3.24e-103 317
MsG0180000009.01.T01 MTR_1g026350 93.464 153 10 0 1 153 1 153 3.07e-100 300
MsG0180000009.01.T01 MTR_5g007640 35.662 544 277 7 26 515 55 579 9.18e-100 313
MsG0180000009.01.T01 MTR_2g095510 37.916 451 236 7 17 430 3 446 1.27e-95 298
MsG0180000009.01.T01 MTR_4g005750 37.925 530 283 9 26 515 11 534 1.57e-95 300
MsG0180000009.01.T01 MTR_3g035130 27.778 504 317 12 44 513 15 505 4.36e-48 174
MsG0180000009.01.T01 MTR_1569s0010 25.725 517 304 11 68 515 22 527 1.01e-43 162
MsG0180000009.01.T01 MTR_8g074040 26.200 500 308 12 67 512 81 573 4.92e-42 159
MsG0180000009.01.T01 MTR_3g064420 26.163 516 310 12 65 515 37 546 2.06e-40 154
MsG0180000009.01.T01 MTR_5g008800 26.220 492 308 10 68 509 40 526 2.28e-40 154
MsG0180000009.01.T01 MTR_3g035130 27.938 451 278 12 44 460 15 452 3.60e-38 146
MsG0180000009.01.T01 MTR_5g076240 26.448 518 302 12 68 519 40 544 1.69e-37 145
MsG0180000009.01.T01 MTR_8g074040 26.164 451 272 12 116 512 12 455 1.69e-35 139
MsG0180000009.01.T01 MTR_5g008820 23.984 492 317 12 68 509 40 524 2.25e-35 139
MsG0180000009.01.T01 MTR_8g074120 26.004 473 281 14 98 512 23 484 6.23e-32 129
MsG0180000009.01.T01 MTR_5g076200 24.754 509 323 9 68 519 40 545 1.42e-31 128
MsG0180000009.01.T01 MTR_2g044180 23.006 539 342 16 49 523 29 558 1.61e-31 128
MsG0180000009.01.T01 MTR_5g076220 24.951 509 322 9 68 519 40 545 2.85e-31 127
MsG0180000009.01.T01 MTR_4g121600 24.242 528 323 18 67 523 45 566 8.58e-31 126
MsG0180000009.01.T01 MTR_4g013210 29.412 357 207 14 194 522 65 404 2.56e-30 122
MsG0180000009.01.T01 MTR_0161s0050 25.265 566 333 14 24 519 2 547 4.51e-30 124
MsG0180000009.01.T01 MTR_4g013210 28.288 403 229 15 164 522 170 556 6.89e-30 123
MsG0180000009.01.T01 MTR_5g023730 23.092 498 325 11 67 512 42 533 1.10e-28 120
MsG0180000009.01.T01 MTR_2g044180 25.201 373 227 11 197 523 101 467 1.26e-28 119
MsG0180000009.01.T01 MTR_0803s0020 24.335 489 310 13 67 509 260 734 1.48e-28 120
MsG0180000009.01.T01 MTR_7g084850 21.756 524 340 12 68 523 203 724 3.66e-28 119
MsG0180000009.01.T01 MTR_4g013210 31.944 216 131 6 319 522 234 445 8.73e-25 107
MsG0180000009.01.T01 MTR_3g088890 60.938 64 25 0 425 488 10 73 1.57e-22 92.4
MsG0180000009.01.T01 MTR_4g011790 29.384 211 139 4 319 522 391 598 2.82e-22 100
MsG0180000009.01.T01 MTR_4g011790 29.384 211 139 4 319 522 400 607 3.08e-22 100
MsG0180000009.01.T01 MTR_2g030240 23.476 443 277 10 69 455 29 465 1.83e-21 98.2
MsG0180000009.01.T01 MTR_4g057755 57.534 73 31 0 379 451 46 118 2.57e-21 90.1
MsG0180000009.01.T01 MTR_1054s0020 23.108 489 324 15 65 517 294 766 1.53e-20 95.9
MsG0180000009.01.T01 MTR_4g011790 28.643 199 132 4 319 510 391 586 7.19e-20 93.6
MsG0180000009.01.T01 MTR_4g013210 32.432 185 109 6 319 491 386 566 2.20e-19 92.0
MsG0180000009.01.T01 MTR_4g013210 28.409 264 156 11 194 440 217 464 1.03e-15 80.1
MsG0180000009.01.T01 MTR_8g083170 30.542 203 123 5 309 498 472 669 3.30e-15 79.0
MsG0180000009.01.T01 MTR_8g083170 25.100 251 149 6 69 284 110 356 6.00e-11 65.5
MsG0180000009.01.T01 MTR_3g084450 33.889 180 104 4 309 475 261 438 8.79e-15 77.4
MsG0180000009.01.T01 MTR_3g084450 33.889 180 104 4 309 475 469 646 1.80e-14 76.6
MsG0180000009.01.T01 MTR_3g084470 33.766 154 89 3 319 461 475 626 2.65e-14 76.3
MsG0180000009.01.T01 MTR_3g084470 25.385 260 150 8 69 298 106 351 9.95e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000009.01.T01 AT1G20510 69.630 540 131 2 14 521 4 542 0.0 760
MsG0180000009.01.T01 AT1G20500 60.759 553 164 6 13 523 9 550 0.0 667
MsG0180000009.01.T01 AT1G20510 67.941 471 118 2 14 452 4 473 0.0 644
MsG0180000009.01.T01 AT1G20480 56.964 560 197 5 4 523 10 565 0.0 637
MsG0180000009.01.T01 AT5G38120 57.630 557 185 8 9 523 3 550 0.0 629
MsG0180000009.01.T01 AT1G20510 67.038 449 115 2 14 430 4 451 0.0 607
MsG0180000009.01.T01 AT5G38120 56.046 521 178 8 9 487 3 514 0.0 571
MsG0180000009.01.T01 AT5G38120 55.967 486 163 8 9 452 3 479 0.0 526
MsG0180000009.01.T01 AT5G38120 55.967 486 163 8 9 452 3 479 0.0 526
MsG0180000009.01.T01 AT1G20490 54.206 428 149 6 13 400 9 429 8.62e-154 447
MsG0180000009.01.T01 AT1G20490 54.206 428 149 6 13 400 9 429 8.62e-154 447
MsG0180000009.01.T01 AT5G63380 40.106 566 271 9 7 519 4 554 3.46e-144 426
MsG0180000009.01.T01 AT4G05160 41.316 547 276 7 17 523 3 544 9.64e-142 419
MsG0180000009.01.T01 AT4G19010 39.531 554 279 9 15 523 20 562 6.90e-141 418
MsG0180000009.01.T01 AT1G65060 38.185 529 282 7 26 515 34 556 3.11e-105 326
MsG0180000009.01.T01 AT3G21240 36.961 533 295 7 21 515 17 546 6.44e-105 325
MsG0180000009.01.T01 AT1G51680 36.813 546 301 6 12 515 10 553 6.49e-96 302
MsG0180000009.01.T01 AT3G21230 36.531 542 291 10 22 515 24 560 2.30e-95 301
MsG0180000009.01.T01 AT1G62940 33.966 527 305 5 26 515 12 532 3.60e-89 284
MsG0180000009.01.T01 AT3G21230 34.933 521 282 11 22 494 24 535 2.12e-82 266
MsG0180000009.01.T01 AT1G51680 34.749 518 294 6 12 487 10 525 4.26e-81 263
MsG0180000009.01.T01 AT3G21240 34.672 473 268 7 21 455 17 486 1.35e-80 260
MsG0180000009.01.T01 AT3G21240 34.672 473 268 7 21 455 17 486 1.35e-80 260
MsG0180000009.01.T01 AT3G21230 35.294 476 259 11 22 451 24 496 2.06e-73 241
MsG0180000009.01.T01 AT1G51680 34.232 482 273 6 12 451 10 489 3.07e-71 236
MsG0180000009.01.T01 AT1G65060 34.305 446 248 7 26 432 34 473 1.23e-66 224
MsG0180000009.01.T01 AT1G21530 26.706 513 315 13 62 519 37 543 1.58e-44 165
MsG0180000009.01.T01 AT1G21530 26.602 515 315 13 62 519 37 545 2.51e-44 164
MsG0180000009.01.T01 AT1G65890 27.203 522 303 15 67 519 39 552 4.45e-43 162
MsG0180000009.01.T01 AT1G76290 26.680 506 308 14 67 519 39 534 1.22e-42 160
MsG0180000009.01.T01 AT1G65880 27.203 522 304 15 67 519 39 553 1.82e-42 160
MsG0180000009.01.T01 AT1G77240 25.000 492 315 9 81 521 55 543 9.02e-42 157
MsG0180000009.01.T01 AT3G48990 27.178 482 309 11 67 513 30 504 1.02e-41 157
MsG0180000009.01.T01 AT1G66120 26.938 516 309 13 67 521 39 547 2.55e-41 157
MsG0180000009.01.T01 AT1G20560 27.000 500 306 13 67 512 41 535 4.20e-41 155
MsG0180000009.01.T01 AT1G21540 26.719 509 311 12 69 521 46 548 1.10e-40 154
MsG0180000009.01.T01 AT5G16370 26.172 512 311 14 74 523 43 549 1.64e-38 148
MsG0180000009.01.T01 AT3G21230 30.542 406 233 11 22 381 24 426 2.05e-37 143
MsG0180000009.01.T01 AT2G17650 26.439 556 323 20 37 519 55 597 3.63e-36 142
MsG0180000009.01.T01 AT1G68270 25.610 492 311 13 67 515 39 518 4.53e-35 138
MsG0180000009.01.T01 AT5G16340 26.772 508 300 16 74 517 43 542 5.51e-35 138
MsG0180000009.01.T01 AT1G20560 26.829 451 271 13 116 512 12 457 3.37e-34 135
MsG0180000009.01.T01 AT1G75960 25.882 510 311 13 68 519 41 541 1.72e-33 134
MsG0180000009.01.T01 AT1G30520 26.739 460 269 14 64 466 34 482 6.55e-29 120
MsG0180000009.01.T01 AT1G30520 26.739 460 269 14 64 466 34 482 6.55e-29 120
MsG0180000009.01.T01 AT3G16910 21.832 513 332 15 67 515 47 554 1.11e-27 117
MsG0180000009.01.T01 AT3G16170 22.895 380 221 8 199 517 234 602 4.85e-27 115
MsG0180000009.01.T01 AT5G36880 21.161 534 343 13 60 523 194 719 2.39e-26 114
MsG0180000009.01.T01 AT5G36880 20.974 534 344 12 60 523 144 669 2.42e-26 114
MsG0180000009.01.T01 AT5G36880 20.974 534 344 12 60 523 144 669 2.42e-26 114
MsG0180000009.01.T01 AT5G36880 20.974 534 344 12 60 523 144 669 2.42e-26 114
MsG0180000009.01.T01 AT5G36880 20.974 534 344 12 60 523 144 669 2.42e-26 114
MsG0180000009.01.T01 AT1G30520 25.397 441 258 15 64 447 34 460 1.24e-21 98.2
MsG0180000009.01.T01 AT1G30520 25.397 441 258 15 64 447 34 460 1.24e-21 98.2
MsG0180000009.01.T01 AT3G23790 25.203 246 153 9 68 284 111 354 4.75e-12 69.3
MsG0180000009.01.T01 AT3G23790 32.515 163 87 5 319 460 480 640 8.22e-11 65.1
MsG0180000009.01.T01 AT5G27600 32.800 125 72 4 319 436 448 567 4.14e-11 66.2

Find 152 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCCCAGCCTCCTTATT+TGG 0.136723 1:-276990 None:intergenic
TGATAGGTTTCTATCGAATT+TGG 0.177748 1:+277330 MsG0180000009.01.T01:three_prime_UTR
CTGCTGCAAAACCCACTTCT+TGG 0.251921 1:-274276 None:intergenic
ACCACTTGTGCCGCCGATAC+TGG 0.271545 1:+275112 MsG0180000009.01.T01:CDS
TGGAGCTTCTACTGAGTCTT+TGG 0.282200 1:+275306 MsG0180000009.01.T01:CDS
TTCTATCGAATTTGGTTAGA+TGG 0.317747 1:+277338 MsG0180000009.01.T01:three_prime_UTR
GTTCCGATGTTTCATATATA+CGG 0.342157 1:+274975 MsG0180000009.01.T01:CDS
TGCTATGGGGCTTCTTGCTA+TGG 0.342915 1:+275010 MsG0180000009.01.T01:CDS
TTAACTGAATCCTCTGGAAT+TGG 0.351281 1:+275286 MsG0180000009.01.T01:CDS
AGCTCACCAGTCCGATTAAC+TGG 0.354985 1:-275415 None:intergenic
CTCTACTCATCAGGAACAAC+TGG 0.359656 1:+274843 MsG0180000009.01.T01:CDS
CAAGTTGTAATGGCTAGATT+TGG 0.372468 1:+274915 MsG0180000009.01.T01:CDS
GGAGGATGACGTCACCTTTA+CGG 0.372564 1:-274497 None:intergenic
TTATATCAGGTATCCAAATA+AGG 0.373402 1:+276977 MsG0180000009.01.T01:intron
CATTAAGTACAAGGGATATC+AGG 0.379179 1:+276559 MsG0180000009.01.T01:CDS
TAAGGAAGCAGGAAGCATTC+AGG 0.399635 1:-277428 None:intergenic
GGTGAATTGGTGGCCGGTGG+AGG 0.402061 1:-274415 None:intergenic
ATGACGGAGAGGCAGACAAC+AGG 0.402334 1:-274546 None:intergenic
AAGGCTGGAAGTAGCTTGTC+AGG 0.410549 1:+277035 MsG0180000009.01.T01:CDS
AGTGAAAGCAAGAACAGGTT+TGG 0.413866 1:-274643 None:intergenic
TTAATCGGACTGGTGAGCTT+TGG 0.414553 1:+275419 MsG0180000009.01.T01:CDS
TGGAGTTGGGAGAGAGGAGG+AGG 0.419067 1:-274515 None:intergenic
GAATTTAGAGAGAACAACTA+TGG 0.419281 1:-275039 None:intergenic
AAGAAACGAACCTTTCATAA+TGG 0.419398 1:-275455 None:intergenic
AGTAGATGGAGTTGGGAGAG+AGG 0.420482 1:-274521 None:intergenic
TTTGTGACAAAATAAAGAAA+TGG 0.421519 1:-277376 None:intergenic
TGGTTTGGTGGAAGTGGAAG+GGG 0.421746 1:-274327 None:intergenic
ACTGGTGAGCTTTGGCTTCG+TGG 0.421933 1:+275427 MsG0180000009.01.T01:CDS
ATCCATCTGGTAAAATTCTT+CGG 0.423771 1:+277252 MsG0180000009.01.T01:CDS
AAAGAGTGGTTTGGTGGAAG+TGG 0.424298 1:-274333 None:intergenic
TCCAAATAAGGAGGCTGGGC+AGG 0.431867 1:+276989 MsG0180000009.01.T01:CDS
TGAAGATGATGATGAGTTGC+TGG 0.434313 1:-274733 None:intergenic
CTCAGCCGTTACCAGTTAAT+CGG 0.436385 1:+275404 MsG0180000009.01.T01:CDS
GGTCTTGCAGTGTTTGCTAT+GGG 0.437424 1:+274996 MsG0180000009.01.T01:CDS
CGGTCTTGCAGTGTTTGCTA+TGG 0.438320 1:+274995 MsG0180000009.01.T01:CDS
GCTATGGGGCTTCTTGCTAT+GGG 0.440856 1:+275011 MsG0180000009.01.T01:CDS
CTACAGCACGCCAGAGTTGT+TGG 0.441513 1:-274440 None:intergenic
GCAGACAACAGGGAAGTAGA+TGG 0.455653 1:-274535 None:intergenic
CTTGTCAGGAAGCCAAGTTA+TGG 0.458124 1:+277049 MsG0180000009.01.T01:CDS
TTGGAAGAGAGTCGTAAGTA+TGG 0.463115 1:+275325 MsG0180000009.01.T01:CDS
AGGCGTCAATGAAGGCGATA+TGG 0.463838 1:-274395 None:intergenic
TTTATTCTCTATAAGGAAGC+AGG 0.470381 1:-277439 None:intergenic
GGGGCTTGTGTCTGCTTCCA+CGG 0.471317 1:+275354 MsG0180000009.01.T01:CDS
TATGGTTTAACTGAATCCTC+TGG 0.471540 1:+275280 MsG0180000009.01.T01:CDS
GTGGTTTGGTGGAAGTGGAA+GGG 0.473586 1:-274328 None:intergenic
AGAGTTGTTGGTAGGTGAAT+TGG 0.474858 1:-274428 None:intergenic
AGGTATCCAAATAAGGAGGC+TGG 0.481674 1:+276984 MsG0180000009.01.T01:intron
TAACCAACCCTCTGGAGTAA+TGG 0.484888 1:-276468 None:intergenic
CGATTTCGCGAGATGTATTG+AGG 0.485277 1:-274602 None:intergenic
GCGAGATGTATTGAGGGGAT+TGG 0.486320 1:-274595 None:intergenic
AACAAAGCCTCTAGTTCTGC+TGG 0.486762 1:-276770 None:intergenic
GCCGGTGGAGGCGTCAATGA+AGG 0.490250 1:-274403 None:intergenic
AGTGGTTTGGTGGAAGTGGA+AGG 0.490859 1:-274329 None:intergenic
GCATATGTGGTAAGGAAGGC+TGG 0.491572 1:+277020 MsG0180000009.01.T01:CDS
ATTAAGAAGATGGAGGTAAA+CGG 0.492454 1:+274222 None:intergenic
TGTATTGAGGGGATTGGTGG+TGG 0.493354 1:-274589 None:intergenic
CGATGGATTCTTATTTGTCG+TGG 0.494381 1:+276520 MsG0180000009.01.T01:CDS
GTGGTGTTCATGAGAATGAT+TGG 0.496058 1:-274711 None:intergenic
GGAATAGTGAAAGCAAGAAC+AGG 0.499098 1:-274648 None:intergenic
CTGCAACAAAATCCATAACT+TGG 0.502133 1:-277061 None:intergenic
ACAGGGAAGTAGATGGAGTT+GGG 0.504299 1:-274528 None:intergenic
TTGGTAGGTGAATTGGTGGC+CGG 0.506146 1:-274421 None:intergenic
GCCACACTACTCTACTCATC+AGG 0.506955 1:+274834 MsG0180000009.01.T01:CDS
AGTCGTAAGTATGGTACGGC+GGG 0.509532 1:+275334 MsG0180000009.01.T01:CDS
AACAGGGAAGTAGATGGAGT+TGG 0.510251 1:-274529 None:intergenic
CGGCGGCACAAGTGGTAAGA+AGG 0.513016 1:-275105 None:intergenic
CATCGCTATGAGATTCTTGT+GGG 0.513470 1:-274889 None:intergenic
GCCTTCATTGACGCCTCCAC+CGG 0.515250 1:+274402 MsG0180000009.01.T01:CDS
GAAGATACTACTCCTTTGCT+TGG 0.520592 1:-274867 None:intergenic
TGCCTCTCCGTCATGTCCCT+CGG 0.521898 1:+274555 MsG0180000009.01.T01:CDS
TTCCTTACCACATATGCCAT+TGG 0.522529 1:-277014 None:intergenic
AGCGATGGTTCAAGTTGTAA+TGG 0.525827 1:+274905 MsG0180000009.01.T01:CDS
CACCTCCTCACTCTCCAACA+TGG 0.527945 1:+274470 MsG0180000009.01.T01:CDS
ACCTCCTCACTCTCCAACAT+GGG 0.533332 1:+274471 MsG0180000009.01.T01:CDS
GTTTGCTATATTGATAGCGA+TGG 0.538596 1:+276503 MsG0180000009.01.T01:CDS
TCCAACATGGGTATCCGTAA+AGG 0.542271 1:+274483 MsG0180000009.01.T01:CDS
ATACAAAAGAATTCGAAAAG+TGG 0.543360 1:+277205 MsG0180000009.01.T01:CDS
GTTGGAGAGTGAGGAGGTGA+CGG 0.543649 1:-274466 None:intergenic
TTCCAATGGCATATGTGGTA+AGG 0.544112 1:+277012 MsG0180000009.01.T01:CDS
ACCTTTACGGATACCCATGT+TGG 0.546570 1:-274484 None:intergenic
ATCTGGTAAAATTCTTCGGA+AGG 0.547613 1:+277256 MsG0180000009.01.T01:CDS
GGAGGCTGGGCAGGTTCCAA+TGG 0.547827 1:+276998 MsG0180000009.01.T01:CDS
ACAGCAGCGTCTACAATAGC+AGG 0.550475 1:-276803 None:intergenic
GTAGGTGAATTGGTGGCCGG+TGG 0.552038 1:-274418 None:intergenic
GAAAGAGCTCATTAAGTACA+AGG 0.552506 1:+276550 MsG0180000009.01.T01:CDS
GCATCTAAAGAGTGGTTTGG+TGG 0.553390 1:-274339 None:intergenic
CGTGGTCCCACCATTATGAA+AGG 0.554145 1:+275445 MsG0180000009.01.T01:CDS
GCAGGTTCCAATGGCATATG+TGG 0.556198 1:+277007 MsG0180000009.01.T01:CDS
ACCAGTATCGGCGGCACAAG+TGG 0.558595 1:-275113 None:intergenic
AAACGAACCTTTCATAATGG+TGG 0.559397 1:-275452 None:intergenic
TTCAGCATAGCAACCAGTAT+CGG 0.561145 1:-275125 None:intergenic
CCAGTCCGATTAACTGGTAA+CGG 0.565679 1:-275409 None:intergenic
GGTATCCAAATAAGGAGGCT+GGG 0.569081 1:+276985 MsG0180000009.01.T01:intron
CCATCGCTATGAGATTCTTG+TGG 0.569419 1:-274890 None:intergenic
GTGGCATCTAAAGAGTGGTT+TGG 0.570177 1:-274342 None:intergenic
CCGTTACCAGTTAATCGGAC+TGG 0.573347 1:+275409 MsG0180000009.01.T01:CDS
AGATGTATTGAGGGGATTGG+TGG 0.576476 1:-274592 None:intergenic
ATCTCCTCCCGCGCTCATCA+CGG 0.577531 1:+274372 MsG0180000009.01.T01:CDS
GAAGCATTCAGGAATGCAGC+AGG 0.582823 1:-277417 None:intergenic
GATTTCGCGAGATGTATTGA+GGG 0.584385 1:-274601 None:intergenic
AATTCACCTACCAACAACTC+TGG 0.585675 1:+274430 MsG0180000009.01.T01:CDS
AACGAACCTTTCATAATGGT+GGG 0.587290 1:-275451 None:intergenic
GATATGGCCGTGATGAGCGC+GGG 0.587698 1:-274379 None:intergenic
AGATGGAGTTGGGAGAGAGG+AGG 0.592217 1:-274518 None:intergenic
GTTGTTGGTAGGTGAATTGG+TGG 0.596032 1:-274425 None:intergenic
AATGGCATATGTGGTAAGGA+AGG 0.598196 1:+277016 MsG0180000009.01.T01:CDS
GTCTTGCAGTGTTTGCTATG+GGG 0.598502 1:+274997 MsG0180000009.01.T01:CDS
CGATATGGCCGTGATGAGCG+CGG 0.598855 1:-274380 None:intergenic
TGACGGAGAGGCAGACAACA+GGG 0.598859 1:-274545 None:intergenic
TTGGAAGTGAAGGTGATGCA+TGG 0.600778 1:-274692 None:intergenic
CGACCATTACTCCAGAGGGT+TGG 0.603546 1:+276465 MsG0180000009.01.T01:CDS
GATAGCGCCGAGGGACATGA+CGG 0.604148 1:-274562 None:intergenic
AGAGTGATCGATCCAAGAAG+TGG 0.604701 1:+274264 MsG0180000009.01.T01:CDS
AGTGACTGAGCGCATCAACC+AGG 0.608319 1:+274803 MsG0180000009.01.T01:CDS
TGGAAGTGAAGGTGATGCAT+GGG 0.609274 1:-274691 None:intergenic
ATGGCCGTGATGAGCGCGGG+AGG 0.610511 1:-274376 None:intergenic
CCACAAGAATCTCATAGCGA+TGG 0.611191 1:+274890 MsG0180000009.01.T01:CDS
GGTCCCTCCAGCAGAACTAG+AGG 0.614140 1:+276763 MsG0180000009.01.T01:intron
GGTGGTGATGATAGCGCCGA+GGG 0.619275 1:-274571 None:intergenic
GATACCCATGTTGGAGAGTG+AGG 0.620102 1:-274475 None:intergenic
ATGAGAATGATTGGAAGTGA+AGG 0.621057 1:-274702 None:intergenic
AGAGAGTCGTAAGTATGGTA+CGG 0.623595 1:+275330 MsG0180000009.01.T01:CDS
AGCGCGGGAGGAGATGAAAG+TGG 0.628660 1:-274364 None:intergenic
GGAACAACTGGACCAAGCAA+AGG 0.634924 1:+274855 MsG0180000009.01.T01:CDS
TCAGTCACTCTACTCAACTC+AGG 0.638010 1:-274789 None:intergenic
GTAGTGTGGCTGTATCATCC+TGG 0.639019 1:-274821 None:intergenic
AAAGCCTCTAGTTCTGCTGG+AGG 0.643862 1:-276767 None:intergenic
AGCATAGCAACCAGTATCGG+CGG 0.644393 1:-275122 None:intergenic
GGCAGGTTATTTCAGTAACG+AGG 0.647450 1:+276430 MsG0180000009.01.T01:intron
ACATCGACCATTACTCCAGA+GGG 0.647619 1:+276461 MsG0180000009.01.T01:CDS
AAAGAGCTCATTAAGTACAA+GGG 0.651964 1:+276551 MsG0180000009.01.T01:CDS
GAGTGATCGATCCAAGAAGT+GGG 0.652473 1:+274265 MsG0180000009.01.T01:CDS
AGACCGTATATATGAAACAT+CGG 0.653796 1:-274978 None:intergenic
GCTTGTGTCTGCTTCCACGG+AGG 0.655096 1:+275357 MsG0180000009.01.T01:CDS
CTCTGGAGTAATGGTCGATG+TGG 0.656546 1:-276459 None:intergenic
AAGCCTCTAGTTCTGCTGGA+GGG 0.656701 1:-276766 None:intergenic
ATTTCGCGAGATGTATTGAG+GGG 0.663874 1:-274600 None:intergenic
GCGGGAGGAGATGAAAGTGG+TGG 0.667975 1:-274361 None:intergenic
AGGTTATTTCAGTAACGAGG+AGG 0.670840 1:+276433 MsG0180000009.01.T01:intron
CGCCGAGGGACATGACGGAG+AGG 0.671164 1:-274557 None:intergenic
AAGTGGTGGCATCTAAAGAG+TGG 0.673146 1:-274347 None:intergenic
TATCAGGTATCCAAATAAGG+AGG 0.676684 1:+276980 MsG0180000009.01.T01:intron
ACCCATGTTGGAGAGTGAGG+AGG 0.679598 1:-274472 None:intergenic
CACATCGACCATTACTCCAG+AGG 0.683319 1:+276460 MsG0180000009.01.T01:CDS
TCCTGATGAGTAGAGTAGTG+TGG 0.691172 1:-274835 None:intergenic
GTCGTAAGTATGGTACGGCG+GGG 0.691371 1:+275335 MsG0180000009.01.T01:CDS
AGCACGCCAGAGTTGTTGGT+AGG 0.696936 1:-274436 None:intergenic
AGTAGAAGCTCCAATTCCAG+AGG 0.697004 1:-275296 None:intergenic
TGGTGGTGATGATAGCGCCG+AGG 0.700534 1:-274572 None:intergenic
GTCAACAATCATAGCCTCCG+TGG 0.700669 1:-275371 None:intergenic
AGATGATGATGAGTTGCTGG+TGG 0.702116 1:-274730 None:intergenic
GAGTCGTAAGTATGGTACGG+CGG 0.704214 1:+275333 MsG0180000009.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATTAATTAAAAA+AGG - Chr1:275613-275632 None:intergenic 0.0%
!! ATTAATTTATATTCTTTGGT+AGG - Chr1:276840-276859 None:intergenic 15.0%
!! GAAAAAAATTAAAGTTGTAT+TGG - Chr1:275811-275830 None:intergenic 15.0%
!!! TAATTTTTTCAATATATAGG+TGG + Chr1:277178-277197 MsG0180000009.01.T01:intron 15.0%
!! AAATGGAATGAAATAATCAT+TGG - Chr1:275938-275957 None:intergenic 20.0%
!! AGTAAAATGTCAAAATTGTT+AGG - Chr1:276143-276162 None:intergenic 20.0%
!! ATAAACAATTCAGATTAGAT+TGG + Chr1:275540-275559 MsG0180000009.01.T01:intron 20.0%
!! TAATTAAAATATTGAACACG+AGG + Chr1:275621-275640 MsG0180000009.01.T01:intron 20.0%
!! TATAGAGAATAAAAATGTTG+TGG + Chr1:277449-277468 MsG0180000009.01.T01:three_prime_UTR 20.0%
!! TTTGTGACAAAATAAAGAAA+TGG - Chr1:277379-277398 None:intergenic 20.0%
!!! ATCGATATTCTAAAAAATGT+GGG - Chr1:276048-276067 None:intergenic 20.0%
!!! CAACATTTTTATTCTCTATA+AGG - Chr1:277449-277468 None:intergenic 20.0%
!!! GTTACTTTTTTTATGTCTAA+TGG - Chr1:276242-276261 None:intergenic 20.0%
!!! TATCGATATTCTAAAAAATG+TGG - Chr1:276049-276068 None:intergenic 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG + Chr1:275889-275908 MsG0180000009.01.T01:intron 20.0%
!!! TTTTGTGTGTATATTATATC+AGG + Chr1:276964-276983 MsG0180000009.01.T01:intron 20.0%
! AAACTCACACAAATAGATAT+TGG + Chr1:276094-276113 MsG0180000009.01.T01:intron 25.0%
! AACTCACACAAATAGATATT+GGG + Chr1:276095-276114 MsG0180000009.01.T01:intron 25.0%
! AATTATAGCATATAAACTCG+TGG - Chr1:275589-275608 None:intergenic 25.0%
! ATACAAAAGAATTCGAAAAG+TGG + Chr1:277205-277224 MsG0180000009.01.T01:CDS 25.0%
! ATATAAGCTCACATAATTGT+TGG + Chr1:276363-276382 MsG0180000009.01.T01:intron 25.0%
! CACTAAAATAATAGATGCAA+AGG + Chr1:276301-276320 MsG0180000009.01.T01:intron 25.0%
! GTAAACAGTAATTTATCTCT+GGG - Chr1:276655-276674 None:intergenic 25.0%
! TCTTTATTACTATTGTGTGT+CGG - Chr1:276677-276696 None:intergenic 25.0%
! TTATATCAGGTATCCAAATA+AGG + Chr1:276977-276996 MsG0180000009.01.T01:intron 25.0%
!! ACTCTTTTTATTAGATGCTT+GGG + Chr1:277122-277141 MsG0180000009.01.T01:intron 25.0%
!! ACTTTTCGAATTCTTTTGTA+TGG - Chr1:277206-277225 None:intergenic 25.0%
!! CAGTTGAACTTTTAAATGAT+AGG + Chr1:277314-277333 MsG0180000009.01.T01:three_prime_UTR 25.0%
!! TGTTTTATGGTTATTGTTAC+AGG + Chr1:276742-276761 MsG0180000009.01.T01:intron 25.0%
!! TTTTAGTGTTATAAACACCT+TGG - Chr1:276289-276308 None:intergenic 25.0%
!!! AAGTGACACTCATTTTAAAA+CGG - Chr1:275745-275764 None:intergenic 25.0%
!!! AATTTTACCAGATGGATTTT+TGG - Chr1:277249-277268 None:intergenic 25.0%
!!! ATTTTACCAGATGGATTTTT+GGG - Chr1:277248-277267 None:intergenic 25.0%
!!! CATAATTGTTGGTTCTATTA+TGG + Chr1:276374-276393 MsG0180000009.01.T01:intron 25.0%
AAAGAGCTCATTAAGTACAA+GGG + Chr1:276551-276570 MsG0180000009.01.T01:CDS 30.0%
AAGAAACGAACCTTTCATAA+TGG - Chr1:275458-275477 None:intergenic 30.0%
ACTCACACAAATAGATATTG+GGG + Chr1:276096-276115 MsG0180000009.01.T01:intron 30.0%
AGACCGTATATATGAAACAT+CGG - Chr1:274981-275000 None:intergenic 30.0%
ATCCATCTGGTAAAATTCTT+CGG + Chr1:277252-277271 MsG0180000009.01.T01:CDS 30.0%
ATTAAAATGCAAGACATGGT+TGG + Chr1:276403-276422 MsG0180000009.01.T01:intron 30.0%
CCTAATGTAAGTATCTTTCA+AGG + Chr1:275256-275275 MsG0180000009.01.T01:intron 30.0%
CCTTGAAAGATACTTACATT+AGG - Chr1:275259-275278 None:intergenic 30.0%
CGTAAATATTGTCAAACTCA+TGG + Chr1:276704-276723 MsG0180000009.01.T01:intron 30.0%
GAATAAAAATGTTGTGGTCT+TGG + Chr1:277455-277474 MsG0180000009.01.T01:three_prime_UTR 30.0%
GAATTTAGAGAGAACAACTA+TGG - Chr1:275042-275061 None:intergenic 30.0%
GCTAATTAAAATGCAAGACA+TGG + Chr1:276399-276418 MsG0180000009.01.T01:intron 30.0%
GGTAAACAGTAATTTATCTC+TGG - Chr1:276656-276675 None:intergenic 30.0%
GTAAGTATCTTTCAAGGTTA+TGG + Chr1:275262-275281 MsG0180000009.01.T01:intron 30.0%
GTTCCGATGTTTCATATATA+CGG + Chr1:274975-274994 MsG0180000009.01.T01:CDS 30.0%
TATACACACAAAACATTCAG+CGG - Chr1:276957-276976 None:intergenic 30.0%
TATATTCTTTGGTAGGCTTA+CGG - Chr1:276833-276852 None:intergenic 30.0%
TCCTTTGAGTAAAGAAGTAA+CGG + Chr1:275213-275232 MsG0180000009.01.T01:intron 30.0%
TGAATTGCTAAACAAGTTTC+CGG - Chr1:275652-275671 None:intergenic 30.0%
TGATAGGTTTCTATCGAATT+TGG + Chr1:277330-277349 MsG0180000009.01.T01:three_prime_UTR 30.0%
TGATTGATAAGAAGGAATAC+TGG - Chr1:276931-276950 None:intergenic 30.0%
TTCTATCGAATTTGGTTAGA+TGG + Chr1:277338-277357 MsG0180000009.01.T01:three_prime_UTR 30.0%
TTTATTCTCTATAAGGAAGC+AGG - Chr1:277442-277461 None:intergenic 30.0%
! CACTCTTTTTATTAGATGCT+TGG + Chr1:277121-277140 MsG0180000009.01.T01:intron 30.0%
!!! GTTCTCTTGCTTTTGTTTTA+TGG + Chr1:276729-276748 MsG0180000009.01.T01:intron 30.0%
AAACGAACCTTTCATAATGG+TGG - Chr1:275455-275474 None:intergenic 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG - Chr1:275836-275855 None:intergenic 35.0%
AACGAACCTTTCATAATGGT+GGG - Chr1:275454-275473 None:intergenic 35.0%
AATGAGAACATGTGGTCTAT+TGG + Chr1:275704-275723 MsG0180000009.01.T01:intron 35.0%
ATCTGGTAAAATTCTTCGGA+AGG + Chr1:277256-277275 MsG0180000009.01.T01:CDS 35.0%
ATGAGAATGATTGGAAGTGA+AGG - Chr1:274705-274724 None:intergenic 35.0%
CAAGTTGTAATGGCTAGATT+TGG + Chr1:274915-274934 MsG0180000009.01.T01:CDS 35.0%
CATTAAGTACAAGGGATATC+AGG + Chr1:276559-276578 MsG0180000009.01.T01:CDS 35.0%
CTGCAACAAAATCCATAACT+TGG - Chr1:277064-277083 None:intergenic 35.0%
GAAAGAGCTCATTAAGTACA+AGG + Chr1:276550-276569 MsG0180000009.01.T01:CDS 35.0%
GAATTGCTAAACAAGTTTCC+GGG - Chr1:275651-275670 None:intergenic 35.0%
TAATACCAGCAACTAACCAA+AGG - Chr1:276342-276361 None:intergenic 35.0%
TAGATGCAAAGGAAAAAGCT+TGG + Chr1:276312-276331 MsG0180000009.01.T01:intron 35.0%
TATCAGGTATCCAAATAAGG+AGG + Chr1:276980-276999 MsG0180000009.01.T01:intron 35.0%
TATGGTTTAACTGAATCCTC+TGG + Chr1:275280-275299 MsG0180000009.01.T01:CDS 35.0%
TCCGTTACTTCTTTACTCAA+AGG - Chr1:275217-275236 None:intergenic 35.0%
TCTATACCCAAAAATCCATC+TGG + Chr1:277239-277258 MsG0180000009.01.T01:CDS 35.0%
TGTGAGAGAAAAGTTTGAGA+GGG - Chr1:275845-275864 None:intergenic 35.0%
TTGATGAGAATGAGAACATG+TGG + Chr1:275696-275715 MsG0180000009.01.T01:intron 35.0%
TTGGCACACAGATTAAGAAA+TGG - Chr1:275955-275974 None:intergenic 35.0%
! TGAGTAAAGAAGTAACGGAA+GGG + Chr1:275218-275237 MsG0180000009.01.T01:intron 35.0%
! TGATTTGAGTTCGCTTCATA+CGG + Chr1:275175-275194 MsG0180000009.01.T01:intron 35.0%
! TTCCGAAGAATTTTACCAGA+TGG - Chr1:277257-277276 None:intergenic 35.0%
! TTGAGTAAAGAAGTAACGGA+AGG + Chr1:275217-275236 MsG0180000009.01.T01:intron 35.0%
!! ATGCATGGGTGATTTTAGAA+AGG - Chr1:274680-274699 None:intergenic 35.0%
!! GTGATTTTAGAAAGGAGTTG+TGG - Chr1:274672-274691 None:intergenic 35.0%
!! GTTTGCTATATTGATAGCGA+TGG + Chr1:276503-276522 MsG0180000009.01.T01:CDS 35.0%
!! TTAACTGAATCCTCTGGAAT+TGG + Chr1:275286-275305 MsG0180000009.01.T01:CDS 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG - Chr1:275980-275999 None:intergenic 35.0%
!!! GACACTCATTTTAAAACGGA+TGG - Chr1:275741-275760 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG + Chr1:275985-276004 MsG0180000009.01.T01:intron 35.0%
AATGGCATATGTGGTAAGGA+AGG + Chr1:277016-277035 MsG0180000009.01.T01:CDS 40.0%
AATTCACCTACCAACAACTC+TGG + Chr1:274430-274449 MsG0180000009.01.T01:CDS 40.0%
AGAGAGTCGTAAGTATGGTA+CGG + Chr1:275330-275349 MsG0180000009.01.T01:CDS 40.0%
AGCGGTCTTGATTGATAAGA+AGG - Chr1:276939-276958 None:intergenic 40.0%
AGTCATGCACGTCTAATGAA+TGG - Chr1:276264-276283 None:intergenic 40.0%
AGTGAAAGCAAGAACAGGTT+TGG - Chr1:274646-274665 None:intergenic 40.0%
ATTTCGCGAGATGTATTGAG+GGG - Chr1:274603-274622 None:intergenic 40.0%
CATCGCTATGAGATTCTTGT+GGG - Chr1:274892-274911 None:intergenic 40.0%
GAAGATACTACTCCTTTGCT+TGG - Chr1:274870-274889 None:intergenic 40.0%
GATTTCGCGAGATGTATTGA+GGG - Chr1:274604-274623 None:intergenic 40.0%
GGAAAGACAAAACATCCAGA+TGG + Chr1:275561-275580 MsG0180000009.01.T01:intron 40.0%
GGAATAGTGAAAGCAAGAAC+AGG - Chr1:274651-274670 None:intergenic 40.0%
GTGGTGTTCATGAGAATGAT+TGG - Chr1:274714-274733 None:intergenic 40.0%
GTGTGAGAGAAAAGTTTGAG+AGG - Chr1:275846-275865 None:intergenic 40.0%
GTTGGTCATATGCTTGACTT+AGG + Chr1:276014-276033 MsG0180000009.01.T01:intron 40.0%
TAGTTGAAGTGATCGAAACG+TGG - Chr1:276627-276646 None:intergenic 40.0%
TATAAACTCGTGGATCCATC+TGG - Chr1:275579-275598 None:intergenic 40.0%
TGAAACAGAGGGAGTAATGT+TGG + Chr1:275996-276015 MsG0180000009.01.T01:intron 40.0%
TTCAGCATAGCAACCAGTAT+CGG - Chr1:275128-275147 None:intergenic 40.0%
TTCCAATGGCATATGTGGTA+AGG + Chr1:277012-277031 MsG0180000009.01.T01:CDS 40.0%
TTCCTTACCACATATGCCAT+TGG - Chr1:277017-277036 None:intergenic 40.0%
TTGGAAGAGAGTCGTAAGTA+TGG + Chr1:275325-275344 MsG0180000009.01.T01:CDS 40.0%
! ACTGTTTGAGTTCGAACTTG+TGG + Chr1:276160-276179 MsG0180000009.01.T01:intron 40.0%
! AGAGTTGTTGGTAGGTGAAT+TGG - Chr1:274431-274450 None:intergenic 40.0%
! AGGTTATTTCAGTAACGAGG+AGG + Chr1:276433-276452 MsG0180000009.01.T01:intron 40.0%
! CGATGGATTCTTATTTGTCG+TGG + Chr1:276520-276539 MsG0180000009.01.T01:CDS 40.0%
! CTGTTTGAGTTCGAACTTGT+GGG + Chr1:276161-276180 MsG0180000009.01.T01:intron 40.0%
! TATGGATTTTGTTGCAGAGC+AGG + Chr1:277067-277086 MsG0180000009.01.T01:CDS 40.0%
! TGAAGATGATGATGAGTTGC+TGG - Chr1:274736-274755 None:intergenic 40.0%
!! AGAGGGTTGGTTAAAAACAG+GGG + Chr1:276478-276497 MsG0180000009.01.T01:CDS 40.0%
!! AGCGATGGTTCAAGTTGTAA+TGG + Chr1:274905-274924 MsG0180000009.01.T01:CDS 40.0%
!! CAGAGGGTTGGTTAAAAACA+GGG + Chr1:276477-276496 MsG0180000009.01.T01:CDS 40.0%
!!! CGACACTCATTTTGAAACAG+AGG + Chr1:275984-276003 MsG0180000009.01.T01:intron 40.0%
AACAGGGAAGTAGATGGAGT+TGG - Chr1:274532-274551 None:intergenic 45.0%
AAGTGGTGGCATCTAAAGAG+TGG - Chr1:274350-274369 None:intergenic 45.0%
ACAGGGAAGTAGATGGAGTT+GGG - Chr1:274531-274550 None:intergenic 45.0%
ACATCGACCATTACTCCAGA+GGG + Chr1:276461-276480 MsG0180000009.01.T01:CDS 45.0%
ACCTTTACGGATACCCATGT+TGG - Chr1:274487-274506 None:intergenic 45.0%
AGAGTGATCGATCCAAGAAG+TGG + Chr1:274264-274283 MsG0180000009.01.T01:CDS 45.0%
AGATATTGGGGATCGAATCC+TGG + Chr1:276108-276127 MsG0180000009.01.T01:intron 45.0%
AGATGTATTGAGGGGATTGG+TGG - Chr1:274595-274614 None:intergenic 45.0%
AGGTATCCAAATAAGGAGGC+TGG + Chr1:276984-277003 MsG0180000009.01.T01:intron 45.0%
AGTAGAAGCTCCAATTCCAG+AGG - Chr1:275299-275318 None:intergenic 45.0%
CCACAAGAATCTCATAGCGA+TGG + Chr1:274890-274909 MsG0180000009.01.T01:CDS 45.0%
CCAGTCCGATTAACTGGTAA+CGG - Chr1:275412-275431 None:intergenic 45.0%
CCATCGCTATGAGATTCTTG+TGG - Chr1:274893-274912 None:intergenic 45.0%
CGATTTCGCGAGATGTATTG+AGG - Chr1:274605-274624 None:intergenic 45.0%
CTCAGCCGTTACCAGTTAAT+CGG + Chr1:275404-275423 MsG0180000009.01.T01:CDS 45.0%
CTCTACTCATCAGGAACAAC+TGG + Chr1:274843-274862 MsG0180000009.01.T01:CDS 45.0%
CTTGTCAGGAAGCCAAGTTA+TGG + Chr1:277049-277068 MsG0180000009.01.T01:CDS 45.0%
GAGTGATCGATCCAAGAAGT+GGG + Chr1:274265-274284 MsG0180000009.01.T01:CDS 45.0%
GGTATCCAAATAAGGAGGCT+GGG + Chr1:276985-277004 MsG0180000009.01.T01:intron 45.0%
GTGGCATCTAAAGAGTGGTT+TGG - Chr1:274345-274364 None:intergenic 45.0%
TATTGAACACGAGGTAGACC+CGG + Chr1:275630-275649 MsG0180000009.01.T01:intron 45.0%
TCAGTCACTCTACTCAACTC+AGG - Chr1:274792-274811 None:intergenic 45.0%
TCCAACATGGGTATCCGTAA+AGG + Chr1:274483-274502 MsG0180000009.01.T01:CDS 45.0%
TCCTGATGAGTAGAGTAGTG+TGG - Chr1:274838-274857 None:intergenic 45.0%
TGGAAGTGAAGGTGATGCAT+GGG - Chr1:274694-274713 None:intergenic 45.0%
TGGAGCTTCTACTGAGTCTT+TGG + Chr1:275306-275325 MsG0180000009.01.T01:CDS 45.0%
TTGGAAGTGAAGGTGATGCA+TGG - Chr1:274695-274714 None:intergenic 45.0%
! AGATGATGATGAGTTGCTGG+TGG - Chr1:274733-274752 None:intergenic 45.0%
! GAAAAAGCTTGGTGAGCCTT+TGG + Chr1:276323-276342 MsG0180000009.01.T01:intron 45.0%
! GCAGAGCAGGTTTGTTTGTT+TGG + Chr1:277080-277099 MsG0180000009.01.T01:intron 45.0%
! GCATCTAAAGAGTGGTTTGG+TGG - Chr1:274342-274361 None:intergenic 45.0%
! GGCAGGTTATTTCAGTAACG+AGG + Chr1:276430-276449 MsG0180000009.01.T01:intron 45.0%
! GTTGTTGGTAGGTGAATTGG+TGG - Chr1:274428-274447 None:intergenic 45.0%
! TTTGAGTTCGCTTCATACGG+TGG + Chr1:275178-275197 MsG0180000009.01.T01:intron 45.0%
!! AAAGAGTGGTTTGGTGGAAG+TGG - Chr1:274336-274355 None:intergenic 45.0%
!! AACAAAGCCTCTAGTTCTGC+TGG - Chr1:276773-276792 None:intergenic 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG - Chr1:275974-275993 None:intergenic 45.0%
!! ATGCAAGACATGGTTGGTGA+TGG + Chr1:276409-276428 MsG0180000009.01.T01:intron 45.0%
!! CCAGAGGGTTGGTTAAAAAC+AGG + Chr1:276476-276495 MsG0180000009.01.T01:CDS 45.0%
!! GGTCTTGCAGTGTTTGCTAT+GGG + Chr1:274996-275015 MsG0180000009.01.T01:CDS 45.0%
!! GTCTTGCAGTGTTTGCTATG+GGG + Chr1:274997-275016 MsG0180000009.01.T01:CDS 45.0%
!! TAACCAACCCTCTGGAGTAA+TGG - Chr1:276471-276490 None:intergenic 45.0%
!! TAAGGAAGCAGGAAGCATTC+AGG - Chr1:277431-277450 None:intergenic 45.0%
!! TTAATCGGACTGGTGAGCTT+TGG + Chr1:275419-275438 MsG0180000009.01.T01:CDS 45.0%
!!! CCTGTTTTTAACCAACCCTC+TGG - Chr1:276479-276498 None:intergenic 45.0%
!! TATAATTAATTTATATTCTT+TGG - Chr1:276844-276863 None:intergenic 5.0%
!!! AATTAATTTTTTCAATATAT+AGG + Chr1:277175-277194 MsG0180000009.01.T01:intron 5.0%
!!! ATTTATTTTTTATAATATGA+TGG + Chr1:276896-276915 MsG0180000009.01.T01:intron 5.0%
ACAGCAGCGTCTACAATAGC+AGG - Chr1:276806-276825 None:intergenic 50.0%
ACCTCCTCACTCTCCAACAT+GGG + Chr1:274471-274490 MsG0180000009.01.T01:CDS 50.0%
AGCTCACCAGTCCGATTAAC+TGG - Chr1:275418-275437 None:intergenic 50.0%
AGGCGTCAATGAAGGCGATA+TGG - Chr1:274398-274417 None:intergenic 50.0%
AGTAGATGGAGTTGGGAGAG+AGG - Chr1:274524-274543 None:intergenic 50.0%
AGTCGTAAGTATGGTACGGC+GGG + Chr1:275334-275353 MsG0180000009.01.T01:CDS 50.0%
ATTGTTAGGCAGAGCACACC+AGG - Chr1:276129-276148 None:intergenic 50.0%
CACATCGACCATTACTCCAG+AGG + Chr1:276460-276479 MsG0180000009.01.T01:CDS 50.0%
CCGTTACCAGTTAATCGGAC+TGG + Chr1:275409-275428 MsG0180000009.01.T01:CDS 50.0%
CGTGGTCCCACCATTATGAA+AGG + Chr1:275445-275464 MsG0180000009.01.T01:CDS 50.0%
CTGCTGCAAAACCCACTTCT+TGG - Chr1:274279-274298 None:intergenic 50.0%
GAGTCGTAAGTATGGTACGG+CGG + Chr1:275333-275352 MsG0180000009.01.T01:CDS 50.0%
GATACCCATGTTGGAGAGTG+AGG - Chr1:274478-274497 None:intergenic 50.0%
GCAGACAACAGGGAAGTAGA+TGG - Chr1:274538-274557 None:intergenic 50.0%
GCAGGTTCCAATGGCATATG+TGG + Chr1:277007-277026 MsG0180000009.01.T01:CDS 50.0%
GCATATGTGGTAAGGAAGGC+TGG + Chr1:277020-277039 MsG0180000009.01.T01:CDS 50.0%
GCCACACTACTCTACTCATC+AGG + Chr1:274834-274853 MsG0180000009.01.T01:CDS 50.0%
GCGAGATGTATTGAGGGGAT+TGG - Chr1:274598-274617 None:intergenic 50.0%
GGAACAACTGGACCAAGCAA+AGG + Chr1:274855-274874 MsG0180000009.01.T01:CDS 50.0%
GGAGGATGACGTCACCTTTA+CGG - Chr1:274500-274519 None:intergenic 50.0%
GTAGTGTGGCTGTATCATCC+TGG - Chr1:274824-274843 None:intergenic 50.0%
GTCAACAATCATAGCCTCCG+TGG - Chr1:275374-275393 None:intergenic 50.0%
TAGACGTGCATGACTCTCCA+AGG + Chr1:276269-276288 MsG0180000009.01.T01:intron 50.0%
TGCTATGGGGCTTCTTGCTA+TGG + Chr1:275010-275029 MsG0180000009.01.T01:CDS 50.0%
TTCGCTTCATACGGTGGTTC+TGG + Chr1:275184-275203 MsG0180000009.01.T01:intron 50.0%
! AAGGCTGGAAGTAGCTTGTC+AGG + Chr1:277035-277054 MsG0180000009.01.T01:CDS 50.0%
! AGCATAGCAACCAGTATCGG+CGG - Chr1:275125-275144 None:intergenic 50.0%
! CGGTCTTGCAGTGTTTGCTA+TGG + Chr1:274995-275014 MsG0180000009.01.T01:CDS 50.0%
! GAAGCATTCAGGAATGCAGC+AGG - Chr1:277420-277439 None:intergenic 50.0%
! GCTATGGGGCTTCTTGCTAT+GGG + Chr1:275011-275030 MsG0180000009.01.T01:CDS 50.0%
! GTGAGCCTTTGGTTAGTTGC+TGG + Chr1:276334-276353 MsG0180000009.01.T01:intron 50.0%
! TGTATTGAGGGGATTGGTGG+TGG - Chr1:274592-274611 None:intergenic 50.0%
! TTGGTAGGTGAATTGGTGGC+CGG - Chr1:274424-274443 None:intergenic 50.0%
!! AAAGCCTCTAGTTCTGCTGG+AGG - Chr1:276770-276789 None:intergenic 50.0%
!! AAGACATGGTTGGTGATGGC+AGG + Chr1:276413-276432 MsG0180000009.01.T01:intron 50.0%
!! AAGCCTCTAGTTCTGCTGGA+GGG - Chr1:276769-276788 None:intergenic 50.0%
!! AGTGGTTTGGTGGAAGTGGA+AGG - Chr1:274332-274351 None:intergenic 50.0%
!! CTCTGGAGTAATGGTCGATG+TGG - Chr1:276462-276481 None:intergenic 50.0%
!! GTGGTTTGGTGGAAGTGGAA+GGG - Chr1:274331-274350 None:intergenic 50.0%
!! TGGTTTGGTGGAAGTGGAAG+GGG - Chr1:274330-274349 None:intergenic 50.0%
ACCCATGTTGGAGAGTGAGG+AGG - Chr1:274475-274494 None:intergenic 55.0%
ACCTGCCCAGCCTCCTTATT+TGG - Chr1:276993-277012 None:intergenic 55.0%
AGATGGAGTTGGGAGAGAGG+AGG - Chr1:274521-274540 None:intergenic 55.0%
AGCACGCCAGAGTTGTTGGT+AGG - Chr1:274439-274458 None:intergenic 55.0%
AGTGACTGAGCGCATCAACC+AGG + Chr1:274803-274822 MsG0180000009.01.T01:CDS 55.0%
CACCTCCTCACTCTCCAACA+TGG + Chr1:274470-274489 MsG0180000009.01.T01:CDS 55.0%
CGACCATTACTCCAGAGGGT+TGG + Chr1:276465-276484 MsG0180000009.01.T01:CDS 55.0%
CTACAGCACGCCAGAGTTGT+TGG - Chr1:274443-274462 None:intergenic 55.0%
GTCGTAAGTATGGTACGGCG+GGG + Chr1:275335-275354 MsG0180000009.01.T01:CDS 55.0%
GTTGGAGAGTGAGGAGGTGA+CGG - Chr1:274469-274488 None:intergenic 55.0%
TCCAAATAAGGAGGCTGGGC+AGG + Chr1:276989-277008 MsG0180000009.01.T01:CDS 55.0%
TGACGGAGAGGCAGACAACA+GGG - Chr1:274548-274567 None:intergenic 55.0%
! ACTGGTGAGCTTTGGCTTCG+TGG + Chr1:275427-275446 MsG0180000009.01.T01:CDS 55.0%
! ATGACGGAGAGGCAGACAAC+AGG - Chr1:274549-274568 None:intergenic 55.0%
!! GCTTCATACGGTGGTTCTGG+TGG + Chr1:275187-275206 MsG0180000009.01.T01:intron 55.0%
ACCACTTGTGCCGCCGATAC+TGG + Chr1:275112-275131 MsG0180000009.01.T01:CDS 60.0%
AGCGCGGGAGGAGATGAAAG+TGG - Chr1:274367-274386 None:intergenic 60.0%
ATCTCCTCCCGCGCTCATCA+CGG + Chr1:274372-274391 MsG0180000009.01.T01:CDS 60.0%
CGATATGGCCGTGATGAGCG+CGG - Chr1:274383-274402 None:intergenic 60.0%
CGGCGGCACAAGTGGTAAGA+AGG - Chr1:275108-275127 None:intergenic 60.0%
GATATGGCCGTGATGAGCGC+GGG - Chr1:274382-274401 None:intergenic 60.0%
GCCTTCATTGACGCCTCCAC+CGG + Chr1:274402-274421 MsG0180000009.01.T01:CDS 60.0%
GCGGGAGGAGATGAAAGTGG+TGG - Chr1:274364-274383 None:intergenic 60.0%
GCTTGTGTCTGCTTCCACGG+AGG + Chr1:275357-275376 MsG0180000009.01.T01:CDS 60.0%
GGGGCTTGTGTCTGCTTCCA+CGG + Chr1:275354-275373 MsG0180000009.01.T01:CDS 60.0%
GGTCCCTCCAGCAGAACTAG+AGG + Chr1:276763-276782 MsG0180000009.01.T01:intron 60.0%
TGCCTCTCCGTCATGTCCCT+CGG + Chr1:274555-274574 MsG0180000009.01.T01:CDS 60.0%
TGGAGTTGGGAGAGAGGAGG+AGG - Chr1:274518-274537 None:intergenic 60.0%
! GATAGCGCCGAGGGACATGA+CGG - Chr1:274565-274584 None:intergenic 60.0%
! GGTGGTGATGATAGCGCCGA+GGG - Chr1:274574-274593 None:intergenic 60.0%
! GTAGGTGAATTGGTGGCCGG+TGG - Chr1:274421-274440 None:intergenic 60.0%
! TGGTGGTGATGATAGCGCCG+AGG - Chr1:274575-274594 None:intergenic 60.0%
!! ACCAGTATCGGCGGCACAAG+TGG - Chr1:275116-275135 None:intergenic 60.0%
GGAGGCTGGGCAGGTTCCAA+TGG + Chr1:276998-277017 MsG0180000009.01.T01:CDS 65.0%
! GGTGAATTGGTGGCCGGTGG+AGG - Chr1:274418-274437 None:intergenic 65.0%
!! GCCGGTGGAGGCGTCAATGA+AGG - Chr1:274406-274425 None:intergenic 65.0%
ATGGCCGTGATGAGCGCGGG+AGG - Chr1:274379-274398 None:intergenic 70.0%
! CGCCGAGGGACATGACGGAG+AGG - Chr1:274560-274579 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 274231 277537 274231 ID=MsG0180000009.01;Name=MsG0180000009.01
Chr1 mRNA 274231 277537 274231 ID=MsG0180000009.01.T01;Parent=MsG0180000009.01;Name=MsG0180000009.01.T01;_AED=0.24;_eAED=0.24;_QI=0|0.8|0.66|1|1|1|6|227|523
Chr1 exon 277197 277537 277197 ID=MsG0180000009.01.T01:exon:7975;Parent=MsG0180000009.01.T01
Chr1 exon 276986 277088 276986 ID=MsG0180000009.01.T01:exon:7974;Parent=MsG0180000009.01.T01
Chr1 exon 276764 276831 276764 ID=MsG0180000009.01.T01:exon:7973;Parent=MsG0180000009.01.T01
Chr1 exon 276435 276580 276435 ID=MsG0180000009.01.T01:exon:7972;Parent=MsG0180000009.01.T01
Chr1 exon 275278 275466 275278 ID=MsG0180000009.01.T01:exon:7971;Parent=MsG0180000009.01.T01
Chr1 exon 274231 275182 274231 ID=MsG0180000009.01.T01:exon:7970;Parent=MsG0180000009.01.T01
Chr1 CDS 274231 275182 274231 ID=MsG0180000009.01.T01:cds;Parent=MsG0180000009.01.T01
Chr1 CDS 275278 275466 275278 ID=MsG0180000009.01.T01:cds;Parent=MsG0180000009.01.T01
Chr1 CDS 276435 276580 276435 ID=MsG0180000009.01.T01:cds;Parent=MsG0180000009.01.T01
Chr1 CDS 276764 276831 276764 ID=MsG0180000009.01.T01:cds;Parent=MsG0180000009.01.T01
Chr1 CDS 276986 277088 276986 ID=MsG0180000009.01.T01:cds;Parent=MsG0180000009.01.T01
Chr1 CDS 277197 277310 277197 ID=MsG0180000009.01.T01:cds;Parent=MsG0180000009.01.T01
Chr1 three_prime_UTR 277311 277537 277311 ID=MsG0180000009.01.T01:three_prime_utr;Parent=MsG0180000009.01.T01
Gene Sequence

>MsG0180000009.01.T01

ATGGAGGTAAACGGTGAGAATACGAATAGCAGAAGAGTGATCGATCCAAGAAGTGGGTTTTGCAGCAGCAATTCAATCTTCTACAGCAAGAGAAAACCCCTTCCACTTCCACCAAACCACTCTTTAGATGCCACCACTTTCATCTCCTCCCGCGCTCATCACGGCCATATCGCCTTCATTGACGCCTCCACCGGCCACCAATTCACCTACCAACAACTCTGGCGTGCTGTAGACTCCGTCACCTCCTCACTCTCCAACATGGGTATCCGTAAAGGTGACGTCATCCTCCTCCTCTCTCCCAACTCCATCTACTTCCCTGTTGTCTGCCTCTCCGTCATGTCCCTCGGCGCTATCATCACCACCACCAATCCCCTCAATACATCTCGCGAAATCGCTAAACAGATCGCTGATTCCAAACCTGTTCTTGCTTTCACTATTCCACAACTCCTTTCTAAAATCACCCATGCATCACCTTCACTTCCAATCATTCTCATGAACACCACCAGCAACTCATCATCATCTTCAACAACAACACTTGATGAAATGATGAAGAAAGAACCTGAGTTGAGTAGAGTGACTGAGCGCATCAACCAGGATGATACAGCCACACTACTCTACTCATCAGGAACAACTGGACCAAGCAAAGGAGTAGTATCTTCCCACAAGAATCTCATAGCGATGGTTCAAGTTGTAATGGCTAGATTTGGTAAAGAACAAGAGACGAGAGAAACTTTCATATGCACTGTTCCGATGTTTCATATATACGGTCTTGCAGTGTTTGCTATGGGGCTTCTTGCTATGGGTTCAACCATAGTTGTTCTCTCTAAATTCGAGATGCATGACATGCTTTCATCTATTGAGAAATTCAGAGCAACCTTCTTACCACTTGTGCCGCCGATACTGGTTGCTATGCTGAACAACGCAGATGCGATTAAGAGAAAGTATGATTTGAGTTATGGTTTAACTGAATCCTCTGGAATTGGAGCTTCTACTGAGTCTTTGGAAGAGAGTCGTAAGTATGGTACGGCGGGGCTTGTGTCTGCTTCCACGGAGGCTATGATTGTTGACACTGAGACTGCTCAGCCGTTACCAGTTAATCGGACTGGTGAGCTTTGGCTTCGTGGTCCCACCATTATGAAAGGTTATTTCAGTAACGAGGAGGCAACCACATCGACCATTACTCCAGAGGGTTGGTTAAAAACAGGGGATGTTTGCTATATTGATAGCGATGGATTCTTATTTGTCGTGGATCGCTTGAAAGAGCTCATTAAGTACAAGGGATATCAGGTCCCTCCAGCAGAACTAGAGGCTTTGTTGCTGACTCATCCTGCTATTGTAGACGCTGCTGTCATACCGTATCCAAATAAGGAGGCTGGGCAGGTTCCAATGGCATATGTGGTAAGGAAGGCTGGAAGTAGCTTGTCAGGAAGCCAAGTTATGGATTTTGTTGCAGAGCAGGTGGCTCCATACAAAAGAATTCGAAAAGTGGCATTTATATCTTCTATACCCAAAAATCCATCTGGTAAAATTCTTCGGAAGGATCTTATCAAGCTAGCTACGTCTAAACTTTGA
Protein sequence

>MsG0180000009.01.T01

MEVNGENTNSRRVIDPRSGFCSSNSIFYSKRKPLPLPPNHSLDATTFISSRAHHGHIAFIDASTGHQFTYQQLWRAVDSVTSSLSNMGIRKGDVILLLSPNSIYFPVVCLSVMSLGAIITTTNPLNTSREIAKQIADSKPVLAFTIPQLLSKITHASPSLPIILMNTTSNSSSSSTTTLDEMMKKEPELSRVTERINQDDTATLLYSSGTTGPSKGVVSSHKNLIAMVQVVMARFGKEQETRETFICTVPMFHIYGLAVFAMGLLAMGSTIVVLSKFEMHDMLSSIEKFRATFLPLVPPILVAMLNNADAIKRKYDLSYGLTESSGIGASTESLEESRKYGTAGLVSASTEAMIVDTETAQPLPVNRTGELWLRGPTIMKGYFSNEEATTSTITPEGWLKTGDVCYIDSDGFLFVVDRLKELIKYKGYQVPPAELEALLLTHPAIVDAAVIPYPNKEAGQVPMAYVVRKAGSSLSGSQVMDFVAEQVAPYKRIRKVAFISSIPKNPSGKILRKDLIKLATSKL*