AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002213.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002213.01.T01 MTR_1g050458 97.093 344 10 0 1 344 1 344 0.0 696
MsG0180002213.01.T01 MTR_1g050458 88.953 344 9 1 1 344 1 315 0.0 619
MsG0180002213.01.T01 MTR_1g060130 62.170 341 128 1 1 340 33 373 9.82e-164 462
MsG0180002213.01.T01 MTR_1g060150 62.170 341 128 1 1 340 33 373 1.07e-162 459
MsG0180002213.01.T01 MTR_2g086290 49.565 345 164 4 1 342 1 338 6.77e-125 362
MsG0180002213.01.T01 MTR_2g086300 45.752 306 160 3 37 341 45 345 8.68e-98 294
MsG0180002213.01.T01 MTR_4g053620 41.193 352 188 6 1 341 1 344 5.98e-96 289
MsG0180002213.01.T01 MTR_3g104770 44.444 306 159 5 41 342 130 428 7.32e-90 276
MsG0180002213.01.T01 MTR_4g060900 29.283 321 186 8 36 324 119 430 5.73e-37 139
MsG0180002213.01.T01 MTR_7g100780 25.895 363 237 6 5 338 121 480 3.42e-36 137
MsG0180002213.01.T01 MTR_2g086330 52.941 51 24 0 274 324 1 51 2.48e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002213.01.T01 AT1G74300 64.742 329 114 2 14 340 16 344 2.47e-155 439
MsG0180002213.01.T01 AT2G36290 59.184 343 139 1 1 342 17 359 2.51e-153 435
MsG0180002213.01.T01 AT2G36290 59.184 343 139 1 1 342 3 345 4.11e-153 434
MsG0180002213.01.T01 AT1G74280 60.125 321 127 1 21 340 41 361 1.51e-142 408
MsG0180002213.01.T01 AT1G74290 59.202 326 128 4 15 336 34 358 1.71e-138 398
MsG0180002213.01.T01 AT3G48410 52.059 340 163 0 2 341 33 372 7.84e-138 397
MsG0180002213.01.T01 AT3G54240 54.354 333 152 0 8 340 12 344 8.05e-138 395
MsG0180002213.01.T01 AT3G48410 52.059 340 163 0 2 341 75 414 2.88e-137 397
MsG0180002213.01.T01 AT5G22460 51.312 343 163 2 1 342 1 340 8.44e-133 382
MsG0180002213.01.T01 AT5G22460 51.312 343 163 2 1 342 1 340 8.44e-133 382
MsG0180002213.01.T01 AT5G22460 51.312 343 163 2 1 342 1 340 8.44e-133 382
MsG0180002213.01.T01 AT1G74300 63.265 245 89 1 97 340 1 245 4.52e-111 323
MsG0180002213.01.T01 AT3G03240 44.702 302 159 4 39 338 35 330 5.16e-94 283
MsG0180002213.01.T01 AT3G44510 43.791 306 162 4 41 341 8 308 6.84e-88 267
MsG0180002213.01.T01 AT3G03230 42.484 306 167 4 39 342 35 333 1.81e-86 264
MsG0180002213.01.T01 AT1G08310 42.038 314 169 5 33 341 2 307 3.20e-85 260
MsG0180002213.01.T01 AT1G08310 42.357 314 165 6 33 341 2 304 4.98e-84 257
MsG0180002213.01.T01 AT3G44510 48.469 196 96 2 41 236 8 198 2.28e-61 196
MsG0180002213.01.T01 AT1G08310 46.890 209 105 3 33 240 2 205 4.74e-61 196
MsG0180002213.01.T01 AT3G44510 37.745 204 120 3 143 341 1 202 4.70e-45 154
MsG0180002213.01.T01 AT3G44510 37.745 204 120 3 143 341 1 202 4.70e-45 154
MsG0180002213.01.T01 AT3G44510 37.745 204 120 3 143 341 1 202 4.70e-45 154
MsG0180002213.01.T01 AT5G02970 23.631 347 236 5 5 324 126 470 2.80e-30 120
MsG0180002213.01.T01 AT3G09690 25.846 325 194 9 36 324 160 473 8.12e-28 114
MsG0180002213.01.T01 AT3G09690 25.846 325 194 9 36 324 160 473 8.12e-28 114

Find 90 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACTTTGATTGTATTATC+TGG 0.206245 1:+34947424 None:intergenic
AATGGGTGGCCAAGCTGTTT+GGG 0.208672 1:-34949043 MsG0180002213.01.T01:CDS
CTTTATCCTTGGTGCTGTTA+TGG 0.220069 1:+34949434 None:intergenic
CAATGGGTGGCCAAGCTGTT+TGG 0.228990 1:-34949044 MsG0180002213.01.T01:CDS
GCTCCCTTAAAAGTGAAAAT+TGG 0.246316 1:-34947109 MsG0180002213.01.T01:three_prime_UTR
TCTGGTGGTTTACTCAAAAT+TGG 0.249224 1:-34947748 MsG0180002213.01.T01:CDS
CGATAATTGGATTTGGAACT+TGG 0.264289 1:-34947480 MsG0180002213.01.T01:CDS
TTTATCCTTGGTGCTGTTAT+GGG 0.264845 1:+34949435 None:intergenic
CTGGTGAACCACATGTTCTT+GGG 0.267606 1:+34949466 None:intergenic
GTTAATTTGCTTTAGATTCT+CGG 0.270263 1:-34947146 MsG0180002213.01.T01:three_prime_UTR
ATATGTCGGGACACGATAAT+TGG 0.282324 1:-34947493 MsG0180002213.01.T01:CDS
TGAAAACAATTGATAAATCT+TGG 0.301425 1:+34947678 None:intergenic
GCACTCACTTCCTCGACATT+TGG 0.310843 1:+34947289 None:intergenic
ATGTTCCATGGCTTACATTC+TGG 0.314084 1:-34947766 MsG0180002213.01.T01:CDS
CCTGGTGAACCACATGTTCT+TGG 0.342532 1:+34949465 None:intergenic
CCTACATCAAGTGTAGTAAA+AGG 0.347381 1:-34947722 MsG0180002213.01.T01:CDS
TGGAACACCATGTTCTTTGT+AGG 0.351078 1:+34949389 None:intergenic
TTAGGGGTTGCTTGAAGAAT+TGG 0.352823 1:-34949211 MsG0180002213.01.T01:intron
CGGGACACGATAATTGGATT+TGG 0.358450 1:-34947487 MsG0180002213.01.T01:CDS
GTCCAGCACCTTGCAATTCA+TGG 0.359218 1:+34947320 None:intergenic
GCTTGCAGATAAGCTGGAAT+TGG 0.360953 1:-34949094 MsG0180002213.01.T01:CDS
GAAAACAATTGATAAATCTT+GGG 0.362671 1:+34947679 None:intergenic
ATACAATCAAAGTTCATCTA+TGG 0.365095 1:-34947417 MsG0180002213.01.T01:CDS
AATCCTCAAAACTCAGTTGA+TGG 0.379935 1:-34947263 MsG0180002213.01.T01:CDS
CCATCTCTCAGCTTTATCCT+TGG 0.381172 1:+34949423 None:intergenic
TTGTGCAGGTTGCTCATAAT+AGG 0.382450 1:+34947817 None:intergenic
ATTGTATTCATACATGGGTT+TGG 0.388797 1:-34949345 MsG0180002213.01.T01:CDS
ATATATCGTTGCAGCGTAGC+AGG 0.408666 1:+34947370 None:intergenic
TAGGGGTTGCTTGAAGAATT+GGG 0.419223 1:-34949210 MsG0180002213.01.T01:CDS
TTCTTGCTAGCTAATTGTCT+TGG 0.429772 1:+34949369 None:intergenic
GAGTTTGGCTCTTGATATTG+AGG 0.442872 1:-34949118 MsG0180002213.01.T01:CDS
GAACTTTGATTGTATTATCT+GGG 0.448382 1:+34947425 None:intergenic
TACCATGAATTGCAAGGTGC+TGG 0.459267 1:-34947322 MsG0180002213.01.T01:CDS
TTGATTGTATTATCTGGGAA+AGG 0.469632 1:+34947430 None:intergenic
GTCTGTTAACAAAGAACATC+AGG 0.473018 1:-34947651 MsG0180002213.01.T01:intron
CACAATTACTTTGGGAAAGA+CGG 0.486713 1:-34949631 None:intergenic
AAGAAGATTGTATTCATACA+TGG 0.487358 1:-34949351 MsG0180002213.01.T01:CDS
TGAGGAGCTTGCAGATAAGC+TGG 0.489202 1:-34949100 MsG0180002213.01.T01:CDS
ATCCTTGGTGCTGTTATGGG+TGG 0.489509 1:+34949438 None:intergenic
TGATTGTATTATCTGGGAAA+GGG 0.492417 1:+34947431 None:intergenic
CTTGCAGATAAGCTGGAATT+GGG 0.497644 1:-34949093 MsG0180002213.01.T01:CDS
GCTGTTATGGGTGGACCACC+TGG 0.508255 1:+34949447 None:intergenic
TTCCATGGCTTACATTCTGG+TGG 0.512659 1:-34947763 MsG0180002213.01.T01:CDS
GCTGAGAGATGGAAGACATA+TGG 0.517259 1:-34949412 MsG0180002213.01.T01:CDS
GAGTTGCTCATTATGTTCCA+TGG 0.517308 1:-34947778 MsG0180002213.01.T01:CDS
TCTTGGGGAGACAGAACAGC+AGG 0.521518 1:+34947695 None:intergenic
ATGGGTGGCCAAGCTGTTTG+GGG 0.530077 1:-34949042 MsG0180002213.01.T01:CDS
TGACTCCTGTGGTAAACTAC+TGG 0.538705 1:-34947874 MsG0180002213.01.T01:CDS
TTTATGACAACAGACTTGCT+GGG 0.539829 1:-34947907 MsG0180002213.01.T01:intron
GCCTCAAATTCATTCCTCAC+AGG 0.540453 1:-34949017 MsG0180002213.01.T01:intron
AGCAACCTGCACAAGACCAG+TGG 0.541485 1:-34947808 MsG0180002213.01.T01:CDS
TGAGCACGAATCTATATGTC+GGG 0.544660 1:-34947506 MsG0180002213.01.T01:CDS
TGAGGCAACCCCAAACAGCT+TGG 0.544790 1:+34949034 None:intergenic
CCAAGAACATGTGGTTCACC+AGG 0.544937 1:-34949465 MsG0180002213.01.T01:CDS
TCATTTGATAGACCAGGGTA+TGG 0.549328 1:-34949174 MsG0180002213.01.T01:CDS
GTGGTAAACTACTGGTGGCA+TGG 0.556169 1:-34947866 MsG0180002213.01.T01:CDS
AGAAGATTGTATTCATACAT+GGG 0.557044 1:-34949350 MsG0180002213.01.T01:CDS
AAAACAATTGATAAATCTTG+GGG 0.558275 1:+34947680 None:intergenic
ACCTGTGAGGAATGAATTTG+AGG 0.560787 1:+34949016 None:intergenic
GATAATTGGATTTGGAACTT+GGG 0.561009 1:-34947479 MsG0180002213.01.T01:CDS
TGGTGAACCACATGTTCTTG+GGG 0.561443 1:+34949467 None:intergenic
AATTCATGGTATTGAATCCA+CGG 0.569030 1:+34947334 None:intergenic
AATTAATTATTACCTTAGGA+AGG 0.569133 1:+34949289 None:intergenic
ATATATATTCACTTGAGTGT+TGG 0.569473 1:-34949605 MsG0180002213.01.T01:five_prime_UTR
GAGCAACTCGAAGTGCCCAC+TGG 0.575657 1:+34947792 None:intergenic
GATTGCAACAAACCTTCCTA+AGG 0.579046 1:-34949301 MsG0180002213.01.T01:intron
AGTGCCCACTGGTCTTGTGC+AGG 0.581921 1:+34947803 None:intergenic
ATCAAAGTTCATCTATGGCA+AGG 0.584167 1:-34947412 MsG0180002213.01.T01:CDS
GTGAGCACGAATCTATATGT+CGG 0.590263 1:-34947507 MsG0180002213.01.T01:CDS
GTTGTGTCATTTGATAGACC+AGG 0.594934 1:-34949180 MsG0180002213.01.T01:CDS
CCAAGGATAAAGCTGAGAGA+TGG 0.598249 1:-34949423 MsG0180002213.01.T01:CDS
CATATGGCCTACAAAGAACA+TGG 0.598581 1:-34949396 MsG0180002213.01.T01:CDS
AAAGGGTTATCAAGATCAAG+TGG 0.600862 1:+34947448 None:intergenic
TATATCGTTGCAGCGTAGCA+GGG 0.601081 1:+34947371 None:intergenic
TTGTGTCATTTGATAGACCA+GGG 0.602205 1:-34949179 MsG0180002213.01.T01:CDS
TGCCACCAGTAGTTTACCAC+AGG 0.607269 1:+34947869 None:intergenic
AACCACCAGAATGTAAGCCA+TGG 0.614041 1:+34947761 None:intergenic
TTATGACAACAGACTTGCTG+GGG 0.632491 1:-34947906 MsG0180002213.01.T01:intron
ACACTTGTTTCCAAATGTCG+AGG 0.640167 1:-34947299 MsG0180002213.01.T01:CDS
GCATCACTTTCTCCATACCC+TGG 0.644710 1:+34949162 None:intergenic
ATTCAATACCATGAATTGCA+AGG 0.645032 1:-34947328 MsG0180002213.01.T01:CDS
CAGTCACAAGTGAAACAACA+AGG 0.646584 1:-34947529 MsG0180002213.01.T01:CDS
CTCCTGTGGTAAACTACTGG+TGG 0.652565 1:-34947871 MsG0180002213.01.T01:CDS
GGTGAACCACATGTTCTTGG+GGG 0.653985 1:+34949468 None:intergenic
GCAACCTGCACAAGACCAGT+GGG 0.658757 1:-34947807 MsG0180002213.01.T01:CDS
AGAACATGTGGTTCACCAGG+TGG 0.673501 1:-34949462 MsG0180002213.01.T01:CDS
AATTAACAGTGTTACCTGTG+AGG 0.684658 1:+34949003 None:intergenic
GGCAACATTGATGACTCCTG+TGG 0.714909 1:-34947885 MsG0180002213.01.T01:CDS
CTTGGGGAGACAGAACAGCA+GGG 0.724794 1:+34947696 None:intergenic
GTCCACCCATAACAGCACCA+AGG 0.749684 1:-34949440 MsG0180002213.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAATAAATTAATAA+TGG - Chr1:34948772-34948791 MsG0180002213.01.T01:intron 0.0%
!!! AATATTTTTATTATTTTTTA+GGG - Chr1:34947883-34947902 MsG0180002213.01.T01:CDS 0.0%
!!! ATTAAAAAAAAAATATTTTA+CGG + Chr1:34947957-34947976 None:intergenic 0.0%
!!! TTATTTATTTATTTTTAATT+TGG - Chr1:34947469-34947488 MsG0180002213.01.T01:CDS 0.0%
!! AAAATAATATTAAAAATGTG+TGG + Chr1:34947747-34947766 None:intergenic 10.0%
!!! AATTTCAGTATTTTTTTTTT+AGG - Chr1:34949499-34949518 MsG0180002213.01.T01:CDS 10.0%
!!! ATTTATTTTTAATTTGGTTA+GGG - Chr1:34947475-34947494 MsG0180002213.01.T01:CDS 10.0%
!!! ATTTCAGTATTTTTTTTTTA+GGG - Chr1:34949500-34949519 MsG0180002213.01.T01:CDS 10.0%
!!! TATTTATTTTTAATTTGGTT+AGG - Chr1:34947474-34947493 MsG0180002213.01.T01:CDS 10.0%
!!! TTTGTATTTTTTTTTTGAAA+GGG - Chr1:34948666-34948685 MsG0180002213.01.T01:intron 10.0%
!! ATAAAACTCAAATTGAATAA+TGG - Chr1:34948482-34948501 MsG0180002213.01.T01:intron 15.0%
!! CTAAATAATTATCAAACTTA+AGG + Chr1:34947442-34947461 None:intergenic 15.0%
!! GTTCTTAATTAAAAATTGAA+AGG - Chr1:34949531-34949550 MsG0180002213.01.T01:CDS 15.0%
!!! AATTGATTTAAATTCAATAC+TGG + Chr1:34948577-34948596 None:intergenic 15.0%
!!! AATTTGAAGATTTTTCATTT+TGG + Chr1:34948172-34948191 None:intergenic 15.0%
!!! GTTTGTATTTTTTTTTTGAA+AGG - Chr1:34948665-34948684 MsG0180002213.01.T01:intron 15.0%
!!! TTTATTTTTAATTTGGTTAG+GGG - Chr1:34947476-34947495 MsG0180002213.01.T01:CDS 15.0%
!! AAAACAATTGATAAATCTTG+GGG + Chr1:34949026-34949045 None:intergenic 20.0%
!! AATTAATTATTACCTTAGGA+AGG + Chr1:34947417-34947436 None:intergenic 20.0%
!! GAAAACAATTGATAAATCTT+GGG + Chr1:34949027-34949046 None:intergenic 20.0%
!! TGAAAAATCTTCAAATTACA+AGG - Chr1:34948175-34948194 MsG0180002213.01.T01:intron 20.0%
!! TGAAAACAATTGATAAATCT+TGG + Chr1:34949028-34949047 None:intergenic 20.0%
!!! AAAATTTGCTCAAATTAACA+GGG + Chr1:34948097-34948116 None:intergenic 20.0%
!!! AATTTTGTTGATTGGAATTT+TGG - Chr1:34947183-34947202 MsG0180002213.01.T01:three_prime_UTR 20.0%
!!! ACCTCTGTAAAATATTTTTT+TGG + Chr1:34948208-34948227 None:intergenic 20.0%
!!! ATGTAAATCATAGTTGTTTT+TGG + Chr1:34948434-34948453 None:intergenic 20.0%
!!! ATTTTGTTGATTGGAATTTT+GGG - Chr1:34947184-34947203 MsG0180002213.01.T01:three_prime_UTR 20.0%
!!! TATTTTACGGGAAAAAAAAA+CGG + Chr1:34947944-34947963 None:intergenic 20.0%
!!! TCCAAAAAAATATTTTACAG+AGG - Chr1:34948204-34948223 MsG0180002213.01.T01:intron 20.0%
!!! TGATTTTACTGTTTAACATT+TGG - Chr1:34949620-34949639 MsG0180002213.01.T01:five_prime_UTR 20.0%
! AAGAAGATTGTATTCATACA+TGG - Chr1:34947352-34947371 MsG0180002213.01.T01:CDS 25.0%
! ACTGTATAAAATCGAAGATA+TGG + Chr1:34948133-34948152 None:intergenic 25.0%
! AGAAGATTGTATTCATACAT+GGG - Chr1:34947353-34947372 MsG0180002213.01.T01:CDS 25.0%
! ATACAATCAAAGTTCATCTA+TGG - Chr1:34949286-34949305 MsG0180002213.01.T01:intron 25.0%
! ATATATATTCACTTGAGTGT+TGG - Chr1:34947098-34947117 MsG0180002213.01.T01:three_prime_UTR 25.0%
! CAAAAAATCTTCGAATTACA+AGG + Chr1:34947985-34948004 None:intergenic 25.0%
! CTTGTAATTTGAGCAAATTT+CGG - Chr1:34948065-34948084 MsG0180002213.01.T01:intron 25.0%
! GAACTTTGATTGTATTATCT+GGG + Chr1:34949281-34949300 None:intergenic 25.0%
! GGTGAATTAATTATTACCTT+AGG + Chr1:34947421-34947440 None:intergenic 25.0%
! GGTTCTTCCAAAAAAAAAAA+AGG - Chr1:34948551-34948570 MsG0180002213.01.T01:intron 25.0%
! TATACAGTCAAAATTCATGA+AGG - Chr1:34948145-34948164 MsG0180002213.01.T01:intron 25.0%
! TGAACTTTGATTGTATTATC+TGG + Chr1:34949282-34949301 None:intergenic 25.0%
! TTAAAGTGTGTAATGAATGT+AGG - Chr1:34948596-34948615 MsG0180002213.01.T01:intron 25.0%
!! AAAAAGAAGTGTAAAGAGTT+TGG - Chr1:34947570-34947589 MsG0180002213.01.T01:intron 25.0%
!! AAACTCTTTACACTTCTTTT+TGG + Chr1:34947571-34947590 None:intergenic 25.0%
!! AAATTTGCTCAAATTAACAG+GGG + Chr1:34948096-34948115 None:intergenic 25.0%
!! AAATTTGCTCAAATTACAAG+GGG + Chr1:34948065-34948084 None:intergenic 25.0%
!! AACAATAGATCTCTGAATTT+TGG + Chr1:34948374-34948393 None:intergenic 25.0%
!! AGATGACTAAAATCTTGTTT+CGG - Chr1:34948530-34948549 MsG0180002213.01.T01:intron 25.0%
!! ATTGCATCAATTTTGTTGAT+TGG - Chr1:34947175-34947194 MsG0180002213.01.T01:three_prime_UTR 25.0%
!! CAAAATTTGCTCAAATTAAC+AGG + Chr1:34948098-34948117 None:intergenic 25.0%
!! CTAAATTTGCTCAAATTACT+GGG + Chr1:34948036-34948055 None:intergenic 25.0%
!! GAAATTTGCTCAAATTACAA+GGG + Chr1:34948066-34948085 None:intergenic 25.0%
!! TAAATTTGCTCAAATTACTG+GGG + Chr1:34948035-34948054 None:intergenic 25.0%
!!! ACTTCCAATTTTCACTTTTA+AGG + Chr1:34949601-34949620 None:intergenic 25.0%
!!! ATACTGGCCTTTTTTTTTTT+TGG + Chr1:34948561-34948580 None:intergenic 25.0%
!!! ATTCGAAGATTTTTTGGTTT+TGG - Chr1:34947989-34948008 MsG0180002213.01.T01:intron 25.0%
!!! CAGTATTTTTTTTTTAGGGA+TGG - Chr1:34949504-34949523 MsG0180002213.01.T01:CDS 25.0%
!!! CTGTTAATTTGAGCAAATTT+TGG - Chr1:34948096-34948115 MsG0180002213.01.T01:intron 25.0%
!!! CTTCCAATTTTCACTTTTAA+GGG + Chr1:34949600-34949619 None:intergenic 25.0%
!!! CTTGTAATTCGAAGATTTTT+TGG - Chr1:34947983-34948002 MsG0180002213.01.T01:intron 25.0%
!!! GTTAATTTGCTTTAGATTCT+CGG - Chr1:34949557-34949576 MsG0180002213.01.T01:exon 25.0%
!!! TGAATACAATTTTTTTCCCA+GGG + Chr1:34948743-34948762 None:intergenic 25.0%
AAAATGCAAAAAGTAAACCC+TGG - Chr1:34948723-34948742 MsG0180002213.01.T01:intron 30.0%
AAATGCAAAAAGTAAACCCT+GGG - Chr1:34948724-34948743 MsG0180002213.01.T01:intron 30.0%
ATAAAATCGAAGATATGGCA+AGG + Chr1:34948128-34948147 None:intergenic 30.0%
ATATTGTTCAAAAACTTCCG+TGG - Chr1:34949352-34949371 MsG0180002213.01.T01:CDS 30.0%
ATTCAATACCATGAATTGCA+AGG - Chr1:34949375-34949394 MsG0180002213.01.T01:CDS 30.0%
ATTGTATTCATACATGGGTT+TGG - Chr1:34947358-34947377 MsG0180002213.01.T01:CDS 30.0%
CCAGTAATTTGAGCAAATTT+AGG - Chr1:34948034-34948053 MsG0180002213.01.T01:intron 30.0%
CGAAAATGACCTATATTACA+GGG - Chr1:34948233-34948252 MsG0180002213.01.T01:intron 30.0%
GAAAATGACCTATATTACAG+GGG - Chr1:34948234-34948253 MsG0180002213.01.T01:intron 30.0%
TAGTCGTTAGATCAGTATAA+AGG + Chr1:34949142-34949161 None:intergenic 30.0%
TCGAAAATGACCTATATTAC+AGG - Chr1:34948232-34948251 MsG0180002213.01.T01:intron 30.0%
TGATTGTATTATCTGGGAAA+GGG + Chr1:34949275-34949294 None:intergenic 30.0%
TTGATTGTATTATCTGGGAA+AGG + Chr1:34949276-34949295 None:intergenic 30.0%
! AAATTTGCTCAAATTACTGG+GGG + Chr1:34948034-34948053 None:intergenic 30.0%
! AATTCATGGTATTGAATCCA+CGG + Chr1:34949372-34949391 None:intergenic 30.0%
! AATTTGCTCAAATTAACAGG+GGG + Chr1:34948095-34948114 None:intergenic 30.0%
! CCTAAATTTGCTCAAATTAC+TGG + Chr1:34948037-34948056 None:intergenic 30.0%
! CGAAATTTGCTCAAATTACA+AGG + Chr1:34948067-34948086 None:intergenic 30.0%
! GATAATTGGATTTGGAACTT+GGG - Chr1:34949224-34949243 MsG0180002213.01.T01:intron 30.0%
! TATGTCATGGGATTTTCAAT+GGG - Chr1:34947643-34947662 MsG0180002213.01.T01:intron 30.0%
! TTATGTCATGGGATTTTCAA+TGG - Chr1:34947642-34947661 MsG0180002213.01.T01:intron 30.0%
!! GGATCTAAGTTTTATGTCAT+GGG - Chr1:34947631-34947650 MsG0180002213.01.T01:intron 30.0%
!!! ATAGTTGTTTTTGGTTGAGT+GGG + Chr1:34948425-34948444 None:intergenic 30.0%
!!! CTGAATACAATTTTTTTCCC+AGG + Chr1:34948744-34948763 None:intergenic 30.0%
!!! TGTTTTAACCCCTGTAATAT+AGG + Chr1:34948245-34948264 None:intergenic 30.0%
AAAAATGTGTGGTCTACGAT+AGG + Chr1:34947736-34947755 None:intergenic 35.0%
AAAGGGTTATCAAGATCAAG+TGG + Chr1:34949258-34949277 None:intergenic 35.0%
AATCCTCAAAACTCAGTTGA+TGG - Chr1:34949440-34949459 MsG0180002213.01.T01:CDS 35.0%
AATTAACAGTGTTACCTGTG+AGG + Chr1:34947703-34947722 None:intergenic 35.0%
ACGGAAATGACCTATATTAC+AGG + Chr1:34947925-34947944 None:intergenic 35.0%
ATCAAAGTTCATCTATGGCA+AGG - Chr1:34949291-34949310 MsG0180002213.01.T01:intron 35.0%
CCTACATCAAGTGTAGTAAA+AGG - Chr1:34948981-34949000 MsG0180002213.01.T01:intron 35.0%
CGGAAATGACCTATATTACA+GGG + Chr1:34947924-34947943 None:intergenic 35.0%
GCTCCCTTAAAAGTGAAAAT+TGG - Chr1:34949594-34949613 MsG0180002213.01.T01:five_prime_UTR 35.0%
GGAAATGACCTATATTACAG+GGG + Chr1:34947923-34947942 None:intergenic 35.0%
GTCTGTTAACAAAGAACATC+AGG - Chr1:34949052-34949071 MsG0180002213.01.T01:CDS 35.0%
GTGTTTAACCCCTGTAATAT+AGG - Chr1:34947912-34947931 MsG0180002213.01.T01:intron 35.0%
TCTGGTGGTTTACTCAAAAT+TGG - Chr1:34948955-34948974 MsG0180002213.01.T01:intron 35.0%
TTCTTGCTAGCTAATTGTCT+TGG + Chr1:34947337-34947356 None:intergenic 35.0%
! CCTTTTACTACACTTGATGT+AGG + Chr1:34948984-34949003 None:intergenic 35.0%
! CGATAATTGGATTTGGAACT+TGG - Chr1:34949223-34949242 MsG0180002213.01.T01:intron 35.0%
! TATCCATCAACTGAGTTTTG+AGG + Chr1:34949446-34949465 None:intergenic 35.0%
! TTGCTCATAATAGGCTTTTG+TGG + Chr1:34948880-34948899 None:intergenic 35.0%
! TTTATGACAACAGACTTGCT+GGG - Chr1:34948796-34948815 MsG0180002213.01.T01:intron 35.0%
! TTTTATGACAACAGACTTGC+TGG - Chr1:34948795-34948814 MsG0180002213.01.T01:intron 35.0%
!! GGCACTAACATAATTTTGAC+TGG - Chr1:34947119-34947138 MsG0180002213.01.T01:three_prime_UTR 35.0%
!! GGGATCTAAGTTTTATGTCA+TGG - Chr1:34947630-34947649 MsG0180002213.01.T01:intron 35.0%
!! TTGTGTCATTTGATAGACCA+GGG - Chr1:34947524-34947543 MsG0180002213.01.T01:CDS 35.0%
!! TTTATCCTTGGTGCTGTTAT+GGG + Chr1:34947271-34947290 None:intergenic 35.0%
!!! CATAGTTGTTTTTGGTTGAG+TGG + Chr1:34948426-34948445 None:intergenic 35.0%
!!! TTTTTGGTTTTGGACCTTCA+TGG - Chr1:34947999-34948018 MsG0180002213.01.T01:intron 35.0%
ACACTTGTTTCCAAATGTCG+AGG - Chr1:34949404-34949423 MsG0180002213.01.T01:CDS 40.0%
ACCTGTGAGGAATGAATTTG+AGG + Chr1:34947690-34947709 None:intergenic 40.0%
ATATGTCGGGACACGATAAT+TGG - Chr1:34949210-34949229 MsG0180002213.01.T01:CDS 40.0%
ATGTTCCATGGCTTACATTC+TGG - Chr1:34948937-34948956 MsG0180002213.01.T01:intron 40.0%
CAGTCACAAGTGAAACAACA+AGG - Chr1:34949174-34949193 MsG0180002213.01.T01:CDS 40.0%
CATATGGCCTACAAAGAACA+TGG - Chr1:34947307-34947326 MsG0180002213.01.T01:CDS 40.0%
CTTGCAGATAAGCTGGAATT+GGG - Chr1:34947610-34947629 MsG0180002213.01.T01:intron 40.0%
GAGTTGCTCATTATGTTCCA+TGG - Chr1:34948925-34948944 MsG0180002213.01.T01:intron 40.0%
GATTGCAACAAACCTTCCTA+AGG - Chr1:34947402-34947421 MsG0180002213.01.T01:CDS 40.0%
GGAAAACGATGATCAAGGTT+AGG + Chr1:34948400-34948419 None:intergenic 40.0%
GTGAGCACGAATCTATATGT+CGG - Chr1:34949196-34949215 MsG0180002213.01.T01:CDS 40.0%
TAGGGGTTGCTTGAAGAATT+GGG - Chr1:34947493-34947512 MsG0180002213.01.T01:CDS 40.0%
TCATTTGATAGACCAGGGTA+TGG - Chr1:34947529-34947548 MsG0180002213.01.T01:CDS 40.0%
TGAGCACGAATCTATATGTC+GGG - Chr1:34949197-34949216 MsG0180002213.01.T01:CDS 40.0%
TGGAACACCATGTTCTTTGT+AGG + Chr1:34947317-34947336 None:intergenic 40.0%
TTAGGGGTTGCTTGAAGAAT+TGG - Chr1:34947492-34947511 MsG0180002213.01.T01:CDS 40.0%
TTGTGCAGGTTGCTCATAAT+AGG + Chr1:34948889-34948908 None:intergenic 40.0%
! GCTTTTGTGGACATGTTGTA+AGG + Chr1:34948867-34948886 None:intergenic 40.0%
!! CTTTATCCTTGGTGCTGTTA+TGG + Chr1:34947272-34947291 None:intergenic 40.0%
!! GAGTTTGGCTCTTGATATTG+AGG - Chr1:34947585-34947604 MsG0180002213.01.T01:intron 40.0%
!! GTTGTGTCATTTGATAGACC+AGG - Chr1:34947523-34947542 MsG0180002213.01.T01:CDS 40.0%
!! TTATGACAACAGACTTGCTG+GGG - Chr1:34948797-34948816 MsG0180002213.01.T01:intron 40.0%
!!! TTGTTTTTGGTTGAGTGGGT+GGG + Chr1:34948421-34948440 None:intergenic 40.0%
AACCACCAGAATGTAAGCCA+TGG + Chr1:34948945-34948964 None:intergenic 45.0%
ATATATCGTTGCAGCGTAGC+AGG + Chr1:34949336-34949355 None:intergenic 45.0%
CCAAGGATAAAGCTGAGAGA+TGG - Chr1:34947280-34947299 MsG0180002213.01.T01:CDS 45.0%
CGGGACACGATAATTGGATT+TGG - Chr1:34949216-34949235 MsG0180002213.01.T01:intron 45.0%
CTGGTGAACCACATGTTCTT+GGG + Chr1:34947240-34947259 None:intergenic 45.0%
GCCTCAAATTCATTCCTCAC+AGG - Chr1:34947686-34947705 MsG0180002213.01.T01:CDS 45.0%
GCTGAGAGATGGAAGACATA+TGG - Chr1:34947291-34947310 MsG0180002213.01.T01:CDS 45.0%
GCTTGCAGATAAGCTGGAAT+TGG - Chr1:34947609-34947628 MsG0180002213.01.T01:intron 45.0%
TACCATGAATTGCAAGGTGC+TGG - Chr1:34949381-34949400 MsG0180002213.01.T01:CDS 45.0%
TATATCGTTGCAGCGTAGCA+GGG + Chr1:34949335-34949354 None:intergenic 45.0%
TGACTCCTGTGGTAAACTAC+TGG - Chr1:34948829-34948848 MsG0180002213.01.T01:intron 45.0%
TGGTGAACCACATGTTCTTG+GGG + Chr1:34947239-34947258 None:intergenic 45.0%
TTCCATGGCTTACATTCTGG+TGG - Chr1:34948940-34948959 MsG0180002213.01.T01:intron 45.0%
! CCATCTCTCAGCTTTATCCT+TGG + Chr1:34947283-34947302 None:intergenic 45.0%
! GGGGGTGAAATTTTCCATGA+AGG + Chr1:34948016-34948035 None:intergenic 45.0%
! GTCATGGGATTTTCAATGGG+TGG - Chr1:34947646-34947665 MsG0180002213.01.T01:intron 45.0%
!!! GTTGTTTTTGGTTGAGTGGG+TGG + Chr1:34948422-34948441 None:intergenic 45.0%
!!! CAATATTTTTATTATTTTTT+AGG - Chr1:34947882-34947901 MsG0180002213.01.T01:CDS 5.0%
!!! GATTTTTTTAAATAAAAATA+GGG + Chr1:34948275-34948294 None:intergenic 5.0%
!!! TGATTTTTTTAAATAAAAAT+AGG + Chr1:34948276-34948295 None:intergenic 5.0%
!!! TTAAAAAAAAAATATTTTAC+GGG + Chr1:34947956-34947975 None:intergenic 5.0%
AATGGGTGGCCAAGCTGTTT+GGG - Chr1:34947660-34947679 MsG0180002213.01.T01:CDS 50.0%
AGAACATGTGGTTCACCAGG+TGG - Chr1:34947241-34947260 MsG0180002213.01.T01:exon 50.0%
CCAAGAACATGTGGTTCACC+AGG - Chr1:34947238-34947257 MsG0180002213.01.T01:exon 50.0%
CCTGGTGAACCACATGTTCT+TGG + Chr1:34947241-34947260 None:intergenic 50.0%
CTCCTGTGGTAAACTACTGG+TGG - Chr1:34948832-34948851 MsG0180002213.01.T01:intron 50.0%
GCACTCACTTCCTCGACATT+TGG + Chr1:34949417-34949436 None:intergenic 50.0%
GCATCACTTTCTCCATACCC+TGG + Chr1:34947544-34947563 None:intergenic 50.0%
GGGTGGGAAAACGATGATCA+AGG + Chr1:34948405-34948424 None:intergenic 50.0%
GGTGAACCACATGTTCTTGG+GGG + Chr1:34947238-34947257 None:intergenic 50.0%
GTCCAGCACCTTGCAATTCA+TGG + Chr1:34949386-34949405 None:intergenic 50.0%
GTGGTAAACTACTGGTGGCA+TGG - Chr1:34948837-34948856 MsG0180002213.01.T01:intron 50.0%
TCTTGGGGGTGGAGGTTTAA+TGG + Chr1:34947224-34947243 None:intergenic 50.0%
TGAGGAGCTTGCAGATAAGC+TGG - Chr1:34947603-34947622 MsG0180002213.01.T01:intron 50.0%
TGCCACCAGTAGTTTACCAC+AGG + Chr1:34948837-34948856 None:intergenic 50.0%
! GGCAACATTGATGACTCCTG+TGG - Chr1:34948818-34948837 MsG0180002213.01.T01:intron 50.0%
!! ATCCTTGGTGCTGTTATGGG+TGG + Chr1:34947268-34947287 None:intergenic 50.0%
AGCAACCTGCACAAGACCAG+TGG - Chr1:34948895-34948914 MsG0180002213.01.T01:intron 55.0%
ATGGGTGGCCAAGCTGTTTG+GGG - Chr1:34947661-34947680 MsG0180002213.01.T01:CDS 55.0%
CAATGGGTGGCCAAGCTGTT+TGG - Chr1:34947659-34947678 MsG0180002213.01.T01:CDS 55.0%
CTTGGGGAGACAGAACAGCA+GGG + Chr1:34949010-34949029 None:intergenic 55.0%
GAACCACATGTTCTTGGGGG+TGG + Chr1:34947235-34947254 None:intergenic 55.0%
GCAACCTGCACAAGACCAGT+GGG - Chr1:34948896-34948915 MsG0180002213.01.T01:intron 55.0%
GTCCACCCATAACAGCACCA+AGG - Chr1:34947263-34947282 MsG0180002213.01.T01:CDS 55.0%
TCTTGGGGAGACAGAACAGC+AGG + Chr1:34949011-34949030 None:intergenic 55.0%
TGAGGCAACCCCAAACAGCT+TGG + Chr1:34947672-34947691 None:intergenic 55.0%
CCACATGTTCTTGGGGGTGG+AGG + Chr1:34947232-34947251 None:intergenic 60.0%
CCTCCACCCCCAAGAACATG+TGG - Chr1:34947229-34947248 MsG0180002213.01.T01:three_prime_UTR 60.0%
GCTGTTATGGGTGGACCACC+TGG + Chr1:34947259-34947278 None:intergenic 60.0%
! GAGCAACTCGAAGTGCCCAC+TGG + Chr1:34948914-34948933 None:intergenic 60.0%
!! AGTGCCCACTGGTCTTGTGC+AGG + Chr1:34948903-34948922 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 34947085 34949640 34947085 ID=MsG0180002213.01;Name=MsG0180002213.01
Chr1 mRNA 34947085 34949640 34947085 ID=MsG0180002213.01.T01;Parent=MsG0180002213.01;Name=MsG0180002213.01.T01;_AED=0.45;_eAED=0.45;_QI=78|1|1|1|1|1|4|167|344
Chr1 exon 34947085 34947548 34947085 ID=MsG0180002213.01.T01:exon:1511;Parent=MsG0180002213.01.T01
Chr1 exon 34947652 34947916 34947652 ID=MsG0180002213.01.T01:exon:1510;Parent=MsG0180002213.01.T01
Chr1 exon 34949018 34949229 34949018 ID=MsG0180002213.01.T01:exon:1509;Parent=MsG0180002213.01.T01
Chr1 exon 34949302 34949640 34949302 ID=MsG0180002213.01.T01:exon:1508;Parent=MsG0180002213.01.T01
Chr1 five_prime_UTR 34949563 34949640 34949563 ID=MsG0180002213.01.T01:five_prime_utr;Parent=MsG0180002213.01.T01
Chr1 CDS 34949302 34949562 34949302 ID=MsG0180002213.01.T01:cds;Parent=MsG0180002213.01.T01
Chr1 CDS 34949018 34949229 34949018 ID=MsG0180002213.01.T01:cds;Parent=MsG0180002213.01.T01
Chr1 CDS 34947652 34947916 34947652 ID=MsG0180002213.01.T01:cds;Parent=MsG0180002213.01.T01
Chr1 CDS 34947252 34947548 34947252 ID=MsG0180002213.01.T01:cds;Parent=MsG0180002213.01.T01
Chr1 three_prime_UTR 34947085 34947251 34947085 ID=MsG0180002213.01.T01:three_prime_utr;Parent=MsG0180002213.01.T01
Gene Sequence

>MsG0180002213.01.T01

ATGTTGAGAAGAATTGCATCAATTTTGTTGATTGGAATTTTGGGTTTAGCATATGAAGCCATTAAACCTCCACCCCCAAGAACATGTGGTTCACCAGGTGGTCCACCCATAACAGCACCAAGGATAAAGCTGAGAGATGGAAGACATATGGCCTACAAAGAACATGGTGTTCCAAGACAATTAGCTAGCAAGAAGATTGTATTCATACATGGGTTTGGTTCTTCTAGACATGATGCAGTGATTGCAACAAACCTTCCTAAGGGGTTGCTTGAAGAATTGGGAGCATATGTTGTGTCATTTGATAGACCAGGGTATGGAGAAAGTGATGCTGATCCAAAAAGAAGTGTAAAGAGTTTGGCTCTTGATATTGAGGAGCTTGCAGATAAGCTGGAATTGGGATCTAAGTTTTATGTCATGGGATTTTCAATGGGTGGCCAAGCTGTTTGGGGTTGCCTCAAATTCATTCCTCACAGACTTGCTGGGGCAACATTGATGACTCCTGTGGTAAACTACTGGTGGCATGGTCTTCCTTACAACATGTCCACAAAAGCCTATTATGAGCAACCTGCACAAGACCAGTGGGCACTTCGAGTTGCTCATTATGTTCCATGGCTTACATTCTGGTGGTTTACTCAAAATTGGTTTCCTACATCAAGTGTAGTAAAAGGCAACCCTGCTGTTCTGTCTCCCCAAGATTTATCAATTGTTTTCAACAAGTCTGTTAACAAAGAACATCAGTCACAAGTGAAACAACAAGGTGAGCACGAATCTATATGTCGGGACACGATAATTGGATTTGGAACTTGGGATTTTGATCCACTTGATCTTGATAACCCTTTCCCAGATAATACAATCAAAGTTCATCTATGGCAAGGTGCTGATGATAAGCTAGTCCCTGCTACGCTGCAACGATATATTGTTCAAAAACTTCCGTGGATTCAATACCATGAATTGCAAGGTGCTGGACACTTGTTTCCAAATGTCGAGGAAGTGAGTGCAGAAATCCTCAAAACTCAGTTGATGGATACAAAATAG
Protein sequence

>MsG0180002213.01.T01

MLRRIASILLIGILGLAYEAIKPPPPRTCGSPGGPPITAPRIKLRDGRHMAYKEHGVPRQLASKKIVFIHGFGSSRHDAVIATNLPKGLLEELGAYVVSFDRPGYGESDADPKRSVKSLALDIEELADKLELGSKFYVMGFSMGGQAVWGCLKFIPHRLAGATLMTPVVNYWWHGLPYNMSTKAYYEQPAQDQWALRVAHYVPWLTFWWFTQNWFPTSSVVKGNPAVLSPQDLSIVFNKSVNKEHQSQVKQQGEHESICRDTIIGFGTWDFDPLDLDNPFPDNTIKVHLWQGADDKLVPATLQRYIVQKLPWIQYHELQGAGHLFPNVEEVSAEILKTQLMDTK*