AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047703.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047703.01.T01 MTR_4g133070 87.248 149 7 3 3 150 4 141 7.16e-64 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047703.01.T01 AT5G20130 53.153 111 50 1 39 149 35 143 1.52e-24 94.4
MsG0880047703.01.T01 AT5G20130 53.153 111 50 1 39 149 64 172 6.19e-24 93.2

Find 39 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTTTGGGTGGGGTAGA+TGG 0.204366 8:-89754117 MsG0880047703.01.T01:CDS
CTGGAGGAGGTTGGTTTGGG+TGG 0.219431 8:-89754126 MsG0880047703.01.T01:CDS
GTGCTGGAGGAGGTTGGTTT+GGG 0.308879 8:-89754129 MsG0880047703.01.T01:CDS
GGTGCTGGAGGAGGTTGGTT+TGG 0.312842 8:-89754130 MsG0880047703.01.T01:CDS
TGGAGGAGGTTGGTTTGGGT+GGG 0.343284 8:-89754125 MsG0880047703.01.T01:CDS
GGAGGAGGTTGGTTTGGGTG+GGG 0.346451 8:-89754124 MsG0880047703.01.T01:CDS
TTTGGGTGGGGTAGATGGTT+TGG 0.362531 8:-89754112 MsG0880047703.01.T01:CDS
CGAGCTGCTTAGTCGTCAAT+TGG 0.372187 8:+89755128 None:intergenic
GGAGGTGACAGCGGCGGTGC+TGG 0.396880 8:-89754145 MsG0880047703.01.T01:CDS
AATCAAAAGACGAGAAGAGC+TGG 0.418830 8:-89754173 MsG0880047703.01.T01:CDS
AACGATGGCGTGACCTGATT+AGG 0.420437 8:+89755152 None:intergenic
CAAGCTAGTCTTACTATACT+TGG 0.438781 8:-89754052 MsG0880047703.01.T01:CDS
TCCCGTATTCAATCCTAATC+AGG 0.439070 8:-89755165 MsG0880047703.01.T01:CDS
CGAGAAGAGCTGGGAGGCGG+AGG 0.440547 8:-89754163 MsG0880047703.01.T01:CDS
GACAGCGGCGGTGCTGGAGG+AGG 0.464114 8:-89754139 MsG0880047703.01.T01:CDS
CAAGCTAGAAAAGCATTAGA+AGG 0.475399 8:-89754241 MsG0880047703.01.T01:CDS
GGTGGGGTAGATGGTTTGGC+TGG 0.477513 8:-89754108 MsG0880047703.01.T01:CDS
AGAAAATTGAATTTGACAAG+TGG 0.484614 8:-89754204 MsG0880047703.01.T01:CDS
CAGTATATCCTAGTTGCAAA+AGG 0.489427 8:-89753910 MsG0880047703.01.T01:CDS
TTGAATACGGGACTCCTCCT+AGG 0.496159 8:+89755176 None:intergenic
GACCTGATTAGGATTGAATA+CGG 0.498106 8:+89755163 None:intergenic
GGTGACAGCGGCGGTGCTGG+AGG 0.514884 8:-89754142 MsG0880047703.01.T01:CDS
GAAAATTGAATTTGACAAGT+GGG 0.514902 8:-89754203 MsG0880047703.01.T01:CDS
GAGCTGCTTAGTCGTCAATT+GGG 0.516872 8:+89755129 None:intergenic
AGACGAGAAGAGCTGGGAGG+CGG 0.517079 8:-89754166 MsG0880047703.01.T01:CDS
GCGGCGGTGCTGGAGGAGGT+TGG 0.517578 8:-89754135 MsG0880047703.01.T01:CDS
AGAGGCATGTGAATCTGCTT+CGG 0.520608 8:+89755053 None:intergenic
GCATTAGAAGGTGCATTAAG+TGG 0.523555 8:-89754229 MsG0880047703.01.T01:CDS
CTGGGAGGCGGAGGTGACAG+CGG 0.571119 8:-89754154 MsG0880047703.01.T01:CDS
GGAGGCGGAGGTGACAGCGG+CGG 0.580483 8:-89754151 MsG0880047703.01.T01:CDS
ATCAAAAGACGAGAAGAGCT+GGG 0.583730 8:-89754172 MsG0880047703.01.T01:CDS
CATTAGAAGGTGCATTAAGT+GGG 0.584288 8:-89754228 MsG0880047703.01.T01:CDS
GGAATGGAAATTGTAAACCT+AGG 0.606359 8:-89755193 MsG0880047703.01.T01:CDS
ACCTGATTAGGATTGAATAC+GGG 0.620878 8:+89755164 None:intergenic
GCATGTGAATCTGCTTCGGT+TGG 0.624681 8:+89755057 None:intergenic
ATGGAAATTGTAAACCTAGG+AGG 0.633667 8:-89755190 MsG0880047703.01.T01:CDS
CTCAGACAACAACAAAAGAG+AGG 0.641852 8:-89755029 MsG0880047703.01.T01:intron
TAGTCGTCAATTGGGAACGA+TGG 0.645640 8:+89755137 None:intergenic
AAAAGACGAGAAGAGCTGGG+AGG 0.769117 8:-89754169 MsG0880047703.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCCATATATTTATTATT+AGG + Chr8:89754424-89754443 None:intergenic 10.0%
!!! TTTATCTTAATTTATTTTGT+TGG - Chr8:89754523-89754542 MsG0880047703.01.T01:intron 10.0%
!! AAGTTGTATCTTATTATATT+CGG + Chr8:89754366-89754385 None:intergenic 15.0%
!! AATTAAATATGTTCTACATT+AGG + Chr8:89754552-89754571 None:intergenic 15.0%
!! ATTGGAATCATAAATAAATT+AGG + Chr8:89754401-89754420 None:intergenic 15.0%
!! CAATCCTAATAATAAATATA+TGG - Chr8:89754417-89754436 MsG0880047703.01.T01:intron 15.0%
!! CATATATTTATTATTAGGAT+TGG + Chr8:89754419-89754438 None:intergenic 15.0%
!! ACAAAAAATAAAACTGAAGT+TGG + Chr8:89754127-89754146 None:intergenic 20.0%
!! ATATGCTATTATAAATAGAG+AGG + Chr8:89754339-89754358 None:intergenic 20.0%
!! CAAAAAATAAAACTGAAGTT+GGG + Chr8:89754126-89754145 None:intergenic 20.0%
!! CAGTGATAAATTAATTTCTA+TGG + Chr8:89754682-89754701 None:intergenic 20.0%
!! TGAATGAATGAATATGAAAT+AGG + Chr8:89754081-89754100 None:intergenic 20.0%
! AGAAAATTGAATTTGACAAG+TGG - Chr8:89754874-89754893 MsG0880047703.01.T01:intron 25.0%
! GAAAATTGAATTTGACAAGT+GGG - Chr8:89754875-89754894 MsG0880047703.01.T01:intron 25.0%
! TAAAGAAATGAATTGCTATG+AGG + Chr8:89754262-89754281 None:intergenic 25.0%
! TAAATATGTTCTACATTAGG+AGG + Chr8:89754549-89754568 None:intergenic 25.0%
!! ATAGTCTCTGATCTAATTTT+GGG - Chr8:89754641-89754660 MsG0880047703.01.T01:intron 25.0%
!! GGTCTAATGATGATAATTTT+TGG - Chr8:89754991-89755010 MsG0880047703.01.T01:intron 25.0%
!! TATAGTCTCTGATCTAATTT+TGG - Chr8:89754640-89754659 MsG0880047703.01.T01:intron 25.0%
AAATTACTACAATACTCGCT+TGG + Chr8:89754212-89754231 None:intergenic 30.0%
AATCAACATAGTATGAGAGT+AGG + Chr8:89754303-89754322 None:intergenic 30.0%
ATACTGCAAATAAAGAGAGA+AGG + Chr8:89755154-89755173 None:intergenic 30.0%
CAAACAAGTTCAGTTAAGAA+AGG + Chr8:89754495-89754514 None:intergenic 30.0%
TATAAGTGAATTCTGATTGC+AGG - Chr8:89754812-89754831 MsG0880047703.01.T01:intron 30.0%
! CTTTCTTAACTGAACTTGTT+TGG - Chr8:89754493-89754512 MsG0880047703.01.T01:intron 30.0%
! TAATTTGTGGTAGCATTTCA+TGG - Chr8:89754226-89754245 MsG0880047703.01.T01:CDS 30.0%
! TAGTCTCTGATCTAATTTTG+GGG - Chr8:89754642-89754661 MsG0880047703.01.T01:intron 30.0%
AATACTCGCTTGGTTATTGT+TGG + Chr8:89754202-89754221 None:intergenic 35.0%
AATTTGAGAAGTGAGTTCTG+AGG - Chr8:89754780-89754799 MsG0880047703.01.T01:intron 35.0%
ACCTGATTAGGATTGAATAC+GGG + Chr8:89753917-89753936 None:intergenic 35.0%
ATGGAAATTGTAAACCTAGG+AGG - Chr8:89753888-89753907 MsG0880047703.01.T01:CDS 35.0%
CAGTATATCCTAGTTGCAAA+AGG - Chr8:89755168-89755187 MsG0880047703.01.T01:CDS 35.0%
CATTATAGTCTCAGATATGG+GGG - Chr8:89754737-89754756 MsG0880047703.01.T01:intron 35.0%
CGCATTATAGTCTCAGATAT+GGG - Chr8:89754735-89754754 MsG0880047703.01.T01:intron 35.0%
GACCTGATTAGGATTGAATA+CGG + Chr8:89753918-89753937 None:intergenic 35.0%
GCATTATAGTCTCAGATATG+GGG - Chr8:89754736-89754755 MsG0880047703.01.T01:intron 35.0%
TGCAATTTACTGTCACACTT+TGG - Chr8:89754463-89754482 MsG0880047703.01.T01:intron 35.0%
! ACTCTTCCGTTGATGAAAAA+TGG - Chr8:89753973-89753992 MsG0880047703.01.T01:intron 35.0%
! CATTAGAAGGTGCATTAAGT+GGG - Chr8:89754850-89754869 MsG0880047703.01.T01:intron 35.0%
! CCATATCTGAGACTATAATG+CGG + Chr8:89754737-89754756 None:intergenic 35.0%
! GCGAGTATTGTAGTAATTTG+TGG - Chr8:89754213-89754232 MsG0880047703.01.T01:CDS 35.0%
! TCAAATCACCTTTTGCAACT+AGG + Chr8:89755179-89755198 None:intergenic 35.0%
!! CAAGCTAGAAAAGCATTAGA+AGG - Chr8:89754837-89754856 MsG0880047703.01.T01:intron 35.0%
!! CAAGCTAGTCTTACTATACT+TGG - Chr8:89755026-89755045 MsG0880047703.01.T01:intron 35.0%
!!! TTTTGTTGTTGTCTGAGAAG+AGG + Chr8:89754046-89754065 None:intergenic 35.0%
AATCAAAAGACGAGAAGAGC+TGG - Chr8:89754905-89754924 MsG0880047703.01.T01:intron 40.0%
AGGGATAAGGCAAATTATGC+AGG + Chr8:89754177-89754196 None:intergenic 40.0%
ATCAAAAGACGAGAAGAGCT+GGG - Chr8:89754906-89754925 MsG0880047703.01.T01:intron 40.0%
CAAATTATGCAGGCAGTGAA+AGG + Chr8:89754167-89754186 None:intergenic 40.0%
CCGCATTATAGTCTCAGATA+TGG - Chr8:89754734-89754753 MsG0880047703.01.T01:intron 40.0%
CTCAGACAACAACAAAAGAG+AGG - Chr8:89754049-89754068 MsG0880047703.01.T01:CDS 40.0%
GACAACAACAAAAGAGAGGT+AGG - Chr8:89754053-89754072 MsG0880047703.01.T01:CDS 40.0%
TCAGATATGGGGGAATTAGT+GGG - Chr8:89754747-89754766 MsG0880047703.01.T01:intron 40.0%
TCCCGTATTCAATCCTAATC+AGG - Chr8:89753913-89753932 MsG0880047703.01.T01:intron 40.0%
! GCATTAGAAGGTGCATTAAG+TGG - Chr8:89754849-89754868 MsG0880047703.01.T01:intron 40.0%
! GTTATTGTTGGCTAGGGATA+AGG + Chr8:89754190-89754209 None:intergenic 40.0%
!! GCGACTCCATTTTTCATCAA+CGG + Chr8:89753982-89754001 None:intergenic 40.0%
CTCAGATATGGGGGAATTAG+TGG - Chr8:89754746-89754765 MsG0880047703.01.T01:intron 45.0%
GAGCTGCTTAGTCGTCAATT+GGG + Chr8:89753952-89753971 None:intergenic 45.0%
TAGTCGTCAATTGGGAACGA+TGG + Chr8:89753944-89753963 None:intergenic 45.0%
! AGAGGCATGTGAATCTGCTT+CGG + Chr8:89754028-89754047 None:intergenic 45.0%
! CGCTTGGTTATTGTTGGCTA+GGG + Chr8:89754196-89754215 None:intergenic 45.0%
! TCGCTTGGTTATTGTTGGCT+AGG + Chr8:89754197-89754216 None:intergenic 45.0%
AAAAGACGAGAAGAGCTGGG+AGG - Chr8:89754909-89754928 MsG0880047703.01.T01:intron 50.0%
AACGATGGCGTGACCTGATT+AGG + Chr8:89753929-89753948 None:intergenic 50.0%
CGAGCTGCTTAGTCGTCAAT+TGG + Chr8:89753953-89753972 None:intergenic 50.0%
GCATGTGAATCTGCTTCGGT+TGG + Chr8:89754024-89754043 None:intergenic 50.0%
TTGAATACGGGACTCCTCCT+AGG + Chr8:89753905-89753924 None:intergenic 50.0%
TTTGGGTGGGGTAGATGGTT+TGG - Chr8:89754966-89754985 MsG0880047703.01.T01:intron 50.0%
!! GTGCTGGAGGAGGTTGGTTT+GGG - Chr8:89754949-89754968 MsG0880047703.01.T01:intron 55.0%
!! GTTGGTTTGGGTGGGGTAGA+TGG - Chr8:89754961-89754980 MsG0880047703.01.T01:intron 55.0%
!! TGGAGGAGGTTGGTTTGGGT+GGG - Chr8:89754953-89754972 MsG0880047703.01.T01:intron 55.0%
AGACGAGAAGAGCTGGGAGG+CGG - Chr8:89754912-89754931 MsG0880047703.01.T01:intron 60.0%
! GGTGCTGGAGGAGGTTGGTT+TGG - Chr8:89754948-89754967 MsG0880047703.01.T01:intron 60.0%
! GGTGGGGTAGATGGTTTGGC+TGG - Chr8:89754970-89754989 MsG0880047703.01.T01:intron 60.0%
!! CTGGAGGAGGTTGGTTTGGG+TGG - Chr8:89754952-89754971 MsG0880047703.01.T01:intron 60.0%
!! GGAGGAGGTTGGTTTGGGTG+GGG - Chr8:89754954-89754973 MsG0880047703.01.T01:intron 60.0%
CGAGAAGAGCTGGGAGGCGG+AGG - Chr8:89754915-89754934 MsG0880047703.01.T01:intron 70.0%
CTGGGAGGCGGAGGTGACAG+CGG - Chr8:89754924-89754943 MsG0880047703.01.T01:intron 70.0%
GGAGGCGGAGGTGACAGCGG+CGG - Chr8:89754927-89754946 MsG0880047703.01.T01:intron 75.0%
GGAGGTGACAGCGGCGGTGC+TGG - Chr8:89754933-89754952 MsG0880047703.01.T01:intron 75.0%
! GACAGCGGCGGTGCTGGAGG+AGG - Chr8:89754939-89754958 MsG0880047703.01.T01:intron 75.0%
! GCGGCGGTGCTGGAGGAGGT+TGG - Chr8:89754943-89754962 MsG0880047703.01.T01:intron 75.0%
! GGTGACAGCGGCGGTGCTGG+AGG - Chr8:89754936-89754955 MsG0880047703.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 89753888 89755212 89753888 ID=MsG0880047703.01;Name=MsG0880047703.01
Chr8 mRNA 89753888 89755212 89753888 ID=MsG0880047703.01.T01;Parent=MsG0880047703.01;Name=MsG0880047703.01.T01;_AED=0.35;_eAED=0.36;_QI=0|0|0|1|0|0|3|0|150
Chr8 exon 89755030 89755212 89755030 ID=MsG0880047703.01.T01:exon:16070;Parent=MsG0880047703.01.T01
Chr8 exon 89754039 89754266 89754039 ID=MsG0880047703.01.T01:exon:16069;Parent=MsG0880047703.01.T01
Chr8 exon 89753888 89753929 89753888 ID=MsG0880047703.01.T01:exon:16068;Parent=MsG0880047703.01.T01
Chr8 CDS 89755030 89755212 89755030 ID=MsG0880047703.01.T01:cds;Parent=MsG0880047703.01.T01
Chr8 CDS 89754039 89754266 89754039 ID=MsG0880047703.01.T01:cds;Parent=MsG0880047703.01.T01
Chr8 CDS 89753888 89753929 89753888 ID=MsG0880047703.01.T01:cds;Parent=MsG0880047703.01.T01
Gene Sequence

>MsG0880047703.01.T01

ATGGAAATTGTAAACCTAGGAGGAGTCCCGTATTCAATCCTAATCAGGTCACGCCATCGTTCCCAATTGACGACTAAGCAGCTCGACTCTTCCGTTGATGAAAAATGGAGTCGCTTACAGCACAAACTCAACACCAACCGAAGCAGATTCACATGCCTCTTCTCAGACAACAACAAAAGAGAGGATCAAGCTAGAAAAGCATTAGAAGGTGCATTAAGTGGGAAGAAAATTGAATTTGACAAGTGGGACAAAGAAATCAAAAGACGAGAAGAGCTGGGAGGCGGAGGTGACAGCGGCGGTGCTGGAGGAGGTTGGTTTGGGTGGGGTAGATGGTTTGGCTGGTCTAATGATGATAATTTTTGGCAAGAAGCAAAACAAGCTAGTCTTACTATACTTGGCATCGTTCTCATATATATCCTAGTTGCAAAAGGTGATTTGATCCTCGCTGCGTAG

Protein sequence

>MsG0880047703.01.T01

MEIVNLGGVPYSILIRSRHRSQLTTKQLDSSVDEKWSRLQHKLNTNRSRFTCLFSDNNKREDQARKALEGALSGKKIEFDKWDKEIKRREELGGGGDSGGAGGGWFGWGRWFGWSNDDNFWQEAKQASLTILGIVLIYILVAKGDLILAA*