AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022514.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022514.01.T01 MTR_4g091320 90.415 313 7 1 1 290 265 577 0.0 565
MsG0480022514.01.T01 MTR_4g007850 90.683 161 12 1 130 290 365 522 7.21e-97 295
MsG0480022514.01.T01 MTR_8g032570 31.142 289 177 8 1 283 285 557 9.58e-34 129
MsG0480022514.01.T01 MTR_8g032570 30.928 291 179 8 1 285 308 582 1.04e-33 129
MsG0480022514.01.T01 MTR_7g076220 30.104 289 180 8 1 283 284 556 8.84e-31 121
MsG0480022514.01.T01 MTR_3g098730 28.302 265 180 6 19 280 290 547 2.86e-24 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022514.01.T01 AT1G61350 59.062 320 100 5 1 290 255 573 1.05e-119 355
MsG0480022514.01.T01 AT2G45720 31.636 275 165 7 14 282 291 548 3.13e-31 122
MsG0480022514.01.T01 AT2G45720 31.636 275 165 7 14 282 291 548 3.13e-31 122
MsG0480022514.01.T01 AT2G45720 31.636 275 165 7 14 282 291 548 3.13e-31 122
MsG0480022514.01.T01 AT2G45720 31.636 275 165 7 14 282 291 548 3.13e-31 122
MsG0480022514.01.T01 AT2G45720 31.636 275 165 7 14 282 291 548 3.13e-31 122
MsG0480022514.01.T01 AT5G50900 32.319 263 172 4 18 280 292 548 4.84e-31 122
MsG0480022514.01.T01 AT1G01830 25.641 312 189 9 2 285 277 573 2.64e-23 99.8
MsG0480022514.01.T01 AT1G01830 25.641 312 189 9 2 285 277 573 2.64e-23 99.8
MsG0480022514.01.T01 AT1G01830 25.641 312 189 9 2 285 277 573 2.64e-23 99.8
MsG0480022514.01.T01 AT2G05810 26.246 301 190 9 9 283 277 571 1.30e-22 97.8
MsG0480022514.01.T01 AT2G05810 26.246 301 190 9 9 283 277 571 1.30e-22 97.8

Find 62 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCTGAATTTCCCTCTTC+TGG 0.157688 4:-73555350 None:intergenic
ACAAACCTCTTTCTATTTCT+AGG 0.200258 4:-73555284 None:intergenic
TGAGGAGGTTAAGAGGTTTA+TGG 0.200986 4:+73554773 MsG0480022514.01.T01:CDS
AAAGTGGCATTTAGATTATC+TGG 0.248155 4:+73555131 MsG0480022514.01.T01:CDS
TGCAAAATCATTTGAAGTTC+GGG 0.266275 4:+73555223 MsG0480022514.01.T01:CDS
TGAAGTTTCGATTCAAGAAT+TGG 0.324307 4:+73555103 MsG0480022514.01.T01:CDS
ATCGAAAGAGGAAGCAATTC+AGG 0.325278 4:+73554836 MsG0480022514.01.T01:CDS
GGATAATGCTTGGGCTGTTT+CGG 0.325581 4:+73554716 MsG0480022514.01.T01:CDS
GCAATTCAGGTGAACTCAAT+TGG 0.339387 4:+73554849 MsG0480022514.01.T01:CDS
ATGCAAAATCATTTGAAGTT+CGG 0.348011 4:+73555222 MsG0480022514.01.T01:CDS
TTCGAAGAATATGACCATTT+CGG 0.350085 4:-73554963 None:intergenic
ATGCTGTGTCGACGTTTATC+AGG 0.351926 4:+73554805 MsG0480022514.01.T01:CDS
GATCCAGAAGAGGGAAATTC+AGG 0.354609 4:+73555347 MsG0480022514.01.T01:CDS
TTCATTCAGAATATTGCCTT+TGG 0.362418 4:+73554873 MsG0480022514.01.T01:CDS
ATCATTTGAAGTTCGGGAAA+TGG 0.365241 4:+73555229 MsG0480022514.01.T01:CDS
GGGCTGTTTCGGCTCATTGT+GGG 0.370420 4:+73554727 MsG0480022514.01.T01:CDS
GATGAAATTGACGGAGAATT+CGG 0.371164 4:+73554695 None:intergenic
CATTGCTTTCTTAGCTTCTT+CGG 0.371612 4:-73555159 None:intergenic
GGTTAAGAGGTTTATGGTTG+AGG 0.384772 4:+73554779 MsG0480022514.01.T01:CDS
GATAAATTCTGCCATAAAAC+CGG 0.390006 4:-73555189 None:intergenic
CGGAGAATTCGGATAATGCT+TGG 0.427171 4:+73554706 MsG0480022514.01.T01:CDS
TTGCTATATTATGCTCGAAA+TGG 0.440057 4:+73555080 MsG0480022514.01.T01:CDS
CCTTTGGAGATGAGTTGGTT+AGG 0.452790 4:+73554889 MsG0480022514.01.T01:CDS
CAATTCAGGTGAACTCAATT+GGG 0.464924 4:+73554850 MsG0480022514.01.T01:CDS
GCAGTATGTTGAATGGAATC+TGG 0.472173 4:+73555537 MsG0480022514.01.T01:CDS
TTGGTGTTGAGGAGGTTAAG+AGG 0.482711 4:+73554766 MsG0480022514.01.T01:CDS
TAAGAGGTTTATGGTTGAGG+AGG 0.487395 4:+73554782 MsG0480022514.01.T01:CDS
TATTGCCTTTGGAGATGAGT+TGG 0.493487 4:+73554884 MsG0480022514.01.T01:CDS
TGGGCTGTTTCGGCTCATTG+TGG 0.496601 4:+73554726 MsG0480022514.01.T01:CDS
ATGGCAGCCGAGGCACTCTC+TGG 0.498131 4:+73555248 MsG0480022514.01.T01:CDS
AGCCGAGGCACTCTCTGGTA+TGG 0.501064 4:+73555253 MsG0480022514.01.T01:CDS
TCAAGAATTGGCTTTGAAAG+TGG 0.504838 4:+73555115 MsG0480022514.01.T01:CDS
GGGGTGTTGAGAAATCTTGT+TGG 0.506061 4:+73554747 MsG0480022514.01.T01:CDS
AGATGAGTTGGTTAGGCAAA+TGG 0.509589 4:+73554896 MsG0480022514.01.T01:CDS
CATACTGCGGAATCTGTTTG+TGG 0.512597 4:-73555522 None:intergenic
GAAGAAGCTAAGAAAGCAAT+GGG 0.516697 4:+73555161 MsG0480022514.01.T01:CDS
GATCAAGTAATTGCAGAAGC+AGG 0.518425 4:-73555328 None:intergenic
AGATTCCGCAGTATGTTGAA+TGG 0.523644 4:+73555530 MsG0480022514.01.T01:CDS
GGAGAATTCGGATAATGCTT+GGG 0.524081 4:+73554707 MsG0480022514.01.T01:CDS
GAGAAATCTTGTTGGTGTTG+AGG 0.526183 4:+73554755 MsG0480022514.01.T01:CDS
CAAATGGTGATTAGAGAAGG+TGG 0.535384 4:+73554912 MsG0480022514.01.T01:CDS
GGCTGTTTCGGCTCATTGTG+GGG 0.543109 4:+73554728 MsG0480022514.01.T01:CDS
AACAAAGGAAATAGCAATGA+GGG 0.544827 4:+73554986 MsG0480022514.01.T01:CDS
CAGGCTTGTGAAATCGAAAG+AGG 0.557597 4:+73554824 MsG0480022514.01.T01:CDS
AAGAAAGCAATGGGAGATGC+CGG 0.558547 4:+73555170 MsG0480022514.01.T01:CDS
GGCACTCTCTGGTATGGTCA+TGG 0.564855 4:+73555259 MsG0480022514.01.T01:CDS
CGAAGAAGCTAAGAAAGCAA+TGG 0.567081 4:+73555160 MsG0480022514.01.T01:CDS
AGGCAAATGGTGATTAGAGA+AGG 0.583520 4:+73554909 MsG0480022514.01.T01:CDS
GCAATTACTTGATCCAGAAG+AGG 0.593346 4:+73555337 MsG0480022514.01.T01:CDS
CAATTACTTGATCCAGAAGA+GGG 0.595894 4:+73555338 MsG0480022514.01.T01:CDS
CTAAAACACGTAACAAAGCA+CGG 0.600884 4:-73554938 None:intergenic
TGACAAGTTGCAATAGTGCA+AGG 0.601203 4:+73555405 MsG0480022514.01.T01:CDS
GTCATATTCTTCGAAAACAA+AGG 0.603999 4:+73554971 MsG0480022514.01.T01:CDS
ATCAAGTAATTGCAGAAGCA+GGG 0.608315 4:-73555327 None:intergenic
AAGGTGTTTGATGAAATTGA+CGG 0.609663 4:+73554686 None:intergenic
TGGTACCTAGAAATAGAAAG+AGG 0.620101 4:+73555279 MsG0480022514.01.T01:CDS
AAATCTTGTTGGTGTTGAGG+AGG 0.623752 4:+73554758 MsG0480022514.01.T01:CDS
CCTAACCAACTCATCTCCAA+AGG 0.624383 4:-73554889 None:intergenic
AGTTCGGGAAATGGCAGCCG+AGG 0.637463 4:+73555238 MsG0480022514.01.T01:CDS
GACCATACCAGAGAGTGCCT+CGG 0.640274 4:-73555255 None:intergenic
AAACAAAGGAAATAGCAATG+AGG 0.655890 4:+73554985 MsG0480022514.01.T01:CDS
AGATTCCATTCAACATACTG+CGG 0.693690 4:-73555535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAGTATTTTAATGAGTTA+TGG + Chr4:73555044-73555063 MsG0480022514.01.T01:CDS 20.0%
!! ATGCAAAATCATTTGAAGTT+CGG + Chr4:73555222-73555241 MsG0480022514.01.T01:CDS 25.0%
!!! TTTAATGAGTTATGGTTTTG+TGG + Chr4:73555052-73555071 MsG0480022514.01.T01:CDS 25.0%
AAACAAAGGAAATAGCAATG+AGG + Chr4:73554985-73555004 MsG0480022514.01.T01:CDS 30.0%
AAAGTGGCATTTAGATTATC+TGG + Chr4:73555131-73555150 MsG0480022514.01.T01:CDS 30.0%
AACAAAGGAAATAGCAATGA+GGG + Chr4:73554986-73555005 MsG0480022514.01.T01:CDS 30.0%
GATAAATTCTGCCATAAAAC+CGG - Chr4:73555192-73555211 None:intergenic 30.0%
GTCATATTCTTCGAAAACAA+AGG + Chr4:73554971-73554990 MsG0480022514.01.T01:CDS 30.0%
TGAAGTTTCGATTCAAGAAT+TGG + Chr4:73555103-73555122 MsG0480022514.01.T01:CDS 30.0%
TTCATTCAGAATATTGCCTT+TGG + Chr4:73554873-73554892 MsG0480022514.01.T01:CDS 30.0%
TTCGAAGAATATGACCATTT+CGG - Chr4:73554966-73554985 None:intergenic 30.0%
TTGCTATATTATGCTCGAAA+TGG + Chr4:73555080-73555099 MsG0480022514.01.T01:CDS 30.0%
! ACAAACCTCTTTCTATTTCT+AGG - Chr4:73555287-73555306 None:intergenic 30.0%
! TGCAAAATCATTTGAAGTTC+GGG + Chr4:73555223-73555242 MsG0480022514.01.T01:CDS 30.0%
!! TGCTAGTTTGTCTATGTTTT+TGG - Chr4:73555456-73555475 None:intergenic 30.0%
AGATTCCATTCAACATACTG+CGG - Chr4:73555538-73555557 None:intergenic 35.0%
AGGAAAAAGATTGTTAGCTC+TGG + Chr4:73555425-73555444 MsG0480022514.01.T01:CDS 35.0%
ATCAAGTAATTGCAGAAGCA+GGG - Chr4:73555330-73555349 None:intergenic 35.0%
CAATTACTTGATCCAGAAGA+GGG + Chr4:73555338-73555357 MsG0480022514.01.T01:CDS 35.0%
CAATTCAGGTGAACTCAATT+GGG + Chr4:73554850-73554869 MsG0480022514.01.T01:CDS 35.0%
CATTGCTTTCTTAGCTTCTT+CGG - Chr4:73555162-73555181 None:intergenic 35.0%
CTAAAACACGTAACAAAGCA+CGG - Chr4:73554941-73554960 None:intergenic 35.0%
GAAGAAGCTAAGAAAGCAAT+GGG + Chr4:73555161-73555180 MsG0480022514.01.T01:CDS 35.0%
TGGTACCTAGAAATAGAAAG+AGG + Chr4:73555279-73555298 MsG0480022514.01.T01:CDS 35.0%
! ATCATTTGAAGTTCGGGAAA+TGG + Chr4:73555229-73555248 MsG0480022514.01.T01:CDS 35.0%
!! TACGTGTTTTAGATCCGAAA+TGG + Chr4:73554949-73554968 MsG0480022514.01.T01:CDS 35.0%
!! TCAAGAATTGGCTTTGAAAG+TGG + Chr4:73555115-73555134 MsG0480022514.01.T01:CDS 35.0%
AGATTCCGCAGTATGTTGAA+TGG + Chr4:73555530-73555549 MsG0480022514.01.T01:CDS 40.0%
AGGCAAATGGTGATTAGAGA+AGG + Chr4:73554909-73554928 MsG0480022514.01.T01:CDS 40.0%
ATCGAAAGAGGAAGCAATTC+AGG + Chr4:73554836-73554855 MsG0480022514.01.T01:CDS 40.0%
CAAATGGTGATTAGAGAAGG+TGG + Chr4:73554912-73554931 MsG0480022514.01.T01:CDS 40.0%
CGAAGAAGCTAAGAAAGCAA+TGG + Chr4:73555160-73555179 MsG0480022514.01.T01:CDS 40.0%
GATCAAGTAATTGCAGAAGC+AGG - Chr4:73555331-73555350 None:intergenic 40.0%
GCAATTACTTGATCCAGAAG+AGG + Chr4:73555337-73555356 MsG0480022514.01.T01:CDS 40.0%
GCAATTCAGGTGAACTCAAT+TGG + Chr4:73554849-73554868 MsG0480022514.01.T01:CDS 40.0%
GCAGTATGTTGAATGGAATC+TGG + Chr4:73555537-73555556 MsG0480022514.01.T01:CDS 40.0%
GGAGAATTCGGATAATGCTT+GGG + Chr4:73554707-73554726 MsG0480022514.01.T01:CDS 40.0%
GGTTAAGAGGTTTATGGTTG+AGG + Chr4:73554779-73554798 MsG0480022514.01.T01:CDS 40.0%
TAAGAGGTTTATGGTTGAGG+AGG + Chr4:73554782-73554801 MsG0480022514.01.T01:CDS 40.0%
TGACAAGTTGCAATAGTGCA+AGG + Chr4:73555405-73555424 MsG0480022514.01.T01:CDS 40.0%
TGAGGAGGTTAAGAGGTTTA+TGG + Chr4:73554773-73554792 MsG0480022514.01.T01:CDS 40.0%
TTACCTGAATTTCCCTCTTC+TGG - Chr4:73555353-73555372 None:intergenic 40.0%
! TATTGCCTTTGGAGATGAGT+TGG + Chr4:73554884-73554903 MsG0480022514.01.T01:CDS 40.0%
!! AAATCTTGTTGGTGTTGAGG+AGG + Chr4:73554758-73554777 MsG0480022514.01.T01:CDS 40.0%
!! AGATGAGTTGGTTAGGCAAA+TGG + Chr4:73554896-73554915 MsG0480022514.01.T01:CDS 40.0%
!! GAGAAATCTTGTTGGTGTTG+AGG + Chr4:73554755-73554774 MsG0480022514.01.T01:CDS 40.0%
AAGAAAGCAATGGGAGATGC+CGG + Chr4:73555170-73555189 MsG0480022514.01.T01:CDS 45.0%
CAGGCTTGTGAAATCGAAAG+AGG + Chr4:73554824-73554843 MsG0480022514.01.T01:CDS 45.0%
CATACTGCGGAATCTGTTTG+TGG - Chr4:73555525-73555544 None:intergenic 45.0%
CCTAACCAACTCATCTCCAA+AGG - Chr4:73554892-73554911 None:intergenic 45.0%
CGGAGAATTCGGATAATGCT+TGG + Chr4:73554706-73554725 MsG0480022514.01.T01:CDS 45.0%
GATCCAGAAGAGGGAAATTC+AGG + Chr4:73555347-73555366 MsG0480022514.01.T01:CDS 45.0%
GGATAATGCTTGGGCTGTTT+CGG + Chr4:73554716-73554735 MsG0480022514.01.T01:CDS 45.0%
! ATGCTGTGTCGACGTTTATC+AGG + Chr4:73554805-73554824 MsG0480022514.01.T01:CDS 45.0%
!! CCTTTGGAGATGAGTTGGTT+AGG + Chr4:73554889-73554908 MsG0480022514.01.T01:CDS 45.0%
!! GGGGTGTTGAGAAATCTTGT+TGG + Chr4:73554747-73554766 MsG0480022514.01.T01:CDS 45.0%
!! TTGGTGTTGAGGAGGTTAAG+AGG + Chr4:73554766-73554785 MsG0480022514.01.T01:CDS 45.0%
!!! AATGGGAGATGCCGGTTTTA+TGG + Chr4:73555178-73555197 MsG0480022514.01.T01:CDS 45.0%
GGCACTCTCTGGTATGGTCA+TGG + Chr4:73555259-73555278 MsG0480022514.01.T01:CDS 55.0%
! GGCTGTTTCGGCTCATTGTG+GGG + Chr4:73554728-73554747 MsG0480022514.01.T01:CDS 55.0%
! GGGCTGTTTCGGCTCATTGT+GGG + Chr4:73554727-73554746 MsG0480022514.01.T01:CDS 55.0%
! TGGGCTGTTTCGGCTCATTG+TGG + Chr4:73554726-73554745 MsG0480022514.01.T01:CDS 55.0%
!! GACCATACCAGAGAGTGCCT+CGG - Chr4:73555258-73555277 None:intergenic 55.0%
AGTTCGGGAAATGGCAGCCG+AGG + Chr4:73555238-73555257 MsG0480022514.01.T01:CDS 60.0%
! AGCCGAGGCACTCTCTGGTA+TGG + Chr4:73555253-73555272 MsG0480022514.01.T01:CDS 60.0%
! ATGGCAGCCGAGGCACTCTC+TGG + Chr4:73555248-73555267 MsG0480022514.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 73554696 73555568 73554696 ID=MsG0480022514.01;Name=MsG0480022514.01
Chr4 mRNA 73554696 73555568 73554696 ID=MsG0480022514.01.T01;Parent=MsG0480022514.01;Name=MsG0480022514.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|290
Chr4 exon 73554696 73555568 73554696 ID=MsG0480022514.01.T01:exon:10318;Parent=MsG0480022514.01.T01
Chr4 CDS 73554696 73555568 73554696 ID=MsG0480022514.01.T01:cds;Parent=MsG0480022514.01.T01
Gene Sequence

>MsG0480022514.01.T01

ATGAAATTGACGGAGAATTCGGATAATGCTTGGGCTGTTTCGGCTCATTGTGGGGTGTTGAGAAATCTTGTTGGTGTTGAGGAGGTTAAGAGGTTTATGGTTGAGGAGGATGCTGTGTCGACGTTTATCAGGCTTGTGAAATCGAAAGAGGAAGCAATTCAGGTGAACTCAATTGGGTTCATTCAGAATATTGCCTTTGGAGATGAGTTGGTTAGGCAAATGGTGATTAGAGAAGGTGGAATCCGTGCTTTGTTACGTGTTTTAGATCCGAAATGGTCATATTCTTCGAAAACAAAGGAAATAGCAATGAGGGCTATTGAAAGTTTGTGTTTTATTTCATCTAGCTCTGTAAGTATTTTAATGAGTTATGGTTTTGTGGATCAGTTGCTATATTATGCTCGAAATGGTGAAGTTTCGATTCAAGAATTGGCTTTGAAAGTGGCATTTAGATTATCTGGAACATCCGAAGAAGCTAAGAAAGCAATGGGAGATGCCGGTTTTATGGCAGAATTTATCAAATTTCTGAATGCAAAATCATTTGAAGTTCGGGAAATGGCAGCCGAGGCACTCTCTGGTATGGTCATGGTACCTAGAAATAGAAAGAGGTTTGTTCAAGATGATCATAACATAGCCCTGCTTCTGCAATTACTTGATCCAGAAGAGGGAAATTCAGGTAACAAAAAGTTCTTAATCTCTATCTTAATGTCATTGACAAGTTGCAATAGTGCAAGGAAAAAGATTGTTAGCTCTGGTTATGCCAAAAACATAGACAAACTAGCAGATGCTGAAGTATCTTGTGATGCTAAAAAACTTGTCAAGAAATTATCCACAAACAGATTCCGCAGTATGTTGAATGGAATCTGGCACTCATGA

Protein sequence

>MsG0480022514.01.T01

MKLTENSDNAWAVSAHCGVLRNLVGVEEVKRFMVEEDAVSTFIRLVKSKEEAIQVNSIGFIQNIAFGDELVRQMVIREGGIRALLRVLDPKWSYSSKTKEIAMRAIESLCFISSSSVSILMSYGFVDQLLYYARNGEVSIQELALKVAFRLSGTSEEAKKAMGDAGFMAEFIKFLNAKSFEVREMAAEALSGMVMVPRNRKRFVQDDHNIALLLQLLDPEEGNSGNKKFLISILMSLTSCNSARKKIVSSGYAKNIDKLADAEVSCDAKKLVKKLSTNRFRSMLNGIWHS*