Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000318.01 | MsG0180002264.01 | 0.850130 | 2.071789e-60 | 4.017271e-57 |
| MsG0180001706.01 | MsG0180002264.01 | 0.819681 | 1.022376e-52 | 7.920747e-50 |
| MsG0180002264.01 | MsG0180004335.01 | 0.822412 | 2.401433e-53 | 2.008645e-50 |
| MsG0180002264.01 | MsG0280007477.01 | 0.826920 | 2.081502e-54 | 1.979408e-51 |
| MsG0180002264.01 | MsG0280009344.01 | 0.807835 | 4.161809e-50 | 2.342138e-47 |
| MsG0180002264.01 | MsG0380014137.01 | 0.856964 | 2.253288e-62 | 5.472856e-59 |
| MsG0180002264.01 | MsG0380016453.01 | 0.800201 | 1.610635e-48 | 7.454316e-46 |
| MsG0180002264.01 | MsG0380017776.01 | 0.814247 | 1.698119e-51 | 1.133543e-48 |
| MsG0180002264.01 | MsG0480019161.01 | 0.817326 | 3.494528e-52 | 2.537085e-49 |
| MsG0180002264.01 | MsG0480019162.01 | 0.824485 | 7.869197e-54 | 6.979483e-51 |
| MsG0180002264.01 | MsG0480021490.01 | 0.805055 | 1.605482e-49 | 8.410868e-47 |
| MsG0180002264.01 | MsG0480024008.01 | 0.812440 | 4.235167e-51 | 2.693164e-48 |
| MsG0180002264.01 | MsG0580030095.01 | 0.806031 | 1.001944e-49 | 5.382246e-47 |
| MsG0180002264.01 | MsG0880043027.01 | 0.881049 | 3.314323e-70 | 1.912430e-66 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAGGCTTGTTGATTGTT+AGG | 0.279375 | 1:-35956265 | None:intergenic |
| TGCTTTGATTCCACATGTTC+AGG | 0.291405 | 1:+35956372 | None:intergenic |
| AAAGGAGAGGATGGTGTTGC+AGG | 0.352797 | 1:-35956481 | MsG0180002264.01.T01:CDS |
| AGGTGATGATATGGGAGGTT+GGG | 0.400904 | 1:-35956401 | MsG0180002264.01.T01:CDS |
| TTCCTTTGCCAATCGGATAT+AGG | 0.424844 | 1:-35956281 | MsG0180002264.01.T01:CDS |
| TATAGGCTTGTTGATTGTTA+GGG | 0.438982 | 1:-35956264 | None:intergenic |
| GTTGCCTTTCCTTTGCCAAT+CGG | 0.455493 | 1:-35956288 | MsG0180002264.01.T01:CDS |
| GAGGTGATGATATGGGAGGT+TGG | 0.475506 | 1:-35956402 | MsG0180002264.01.T01:CDS |
| AAGGAGAGGATGGTGTTGCA+GGG | 0.524192 | 1:-35956480 | MsG0180002264.01.T01:CDS |
| AGCCTATATCCGATTGGCAA+AGG | 0.540840 | 1:+35956279 | None:intergenic |
| ACCTCAGCAAAATAGTTCCC+AGG | 0.559019 | 1:+35956420 | None:intergenic |
| TGAGATGACATCGGAGTCAA+AGG | 0.560404 | 1:-35956499 | None:intergenic |
| ATCGGAGTCAAAGGAGAGGA+TGG | 0.581000 | 1:-35956490 | MsG0180002264.01.T01:CDS |
| TCAACAAGCCTATATCCGAT+TGG | 0.607529 | 1:+35956273 | None:intergenic |
| TGGGCTTGTGCCTGAACATG+TGG | 0.607560 | 1:-35956382 | MsG0180002264.01.T01:CDS |
| ATATCCGATTGGCAAAGGAA+AGG | 0.630037 | 1:+35956284 | None:intergenic |
| AGGAGAGGATGGTGTTGCAG+GGG | 0.633863 | 1:-35956479 | MsG0180002264.01.T01:CDS |
| TGCTGAGGTGATGATATGGG+AGG | 0.653250 | 1:-35956406 | MsG0180002264.01.T01:CDS |
| TGACATCGGAGTCAAAGGAG+AGG | 0.666954 | 1:-35956494 | MsG0180002264.01.T01:CDS |
| TGTTGATGCTGAGATGACAT+CGG | 0.739255 | 1:-35956508 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTTTGCTGAGGTGATGATA+TGG | - | Chr1:35956351-35956370 | MsG0180002264.01.T01:CDS | 35.0% |
| ! | TTTTGCTGAGGTGATGATAT+GGG | - | Chr1:35956352-35956371 | MsG0180002264.01.T01:CDS | 35.0% |
| !! | ACTTGCTGAGATGAATTTTG+TGG | - | Chr1:35956313-35956332 | MsG0180002264.01.T01:CDS | 35.0% |
| ATATCCGATTGGCAAAGGAA+AGG | + | Chr1:35956480-35956499 | None:intergenic | 40.0% | |
| TCAACAAGCCTATATCCGAT+TGG | + | Chr1:35956491-35956510 | None:intergenic | 40.0% | |
| TGCTTTGATTCCACATGTTC+AGG | + | Chr1:35956392-35956411 | None:intergenic | 40.0% | |
| TTCCTTTGCCAATCGGATAT+AGG | - | Chr1:35956480-35956499 | MsG0180002264.01.T01:CDS | 40.0% | |
| ACCTCAGCAAAATAGTTCCC+AGG | + | Chr1:35956344-35956363 | None:intergenic | 45.0% | |
| AGCCTATATCCGATTGGCAA+AGG | + | Chr1:35956485-35956504 | None:intergenic | 45.0% | |
| AGGTGATGATATGGGAGGTT+GGG | - | Chr1:35956360-35956379 | MsG0180002264.01.T01:CDS | 45.0% | |
| GTTGCCTTTCCTTTGCCAAT+CGG | - | Chr1:35956473-35956492 | MsG0180002264.01.T01:CDS | 45.0% | |
| ! | ATGAATTTTGTGGTTGCGCC+TGG | - | Chr1:35956323-35956342 | MsG0180002264.01.T01:CDS | 45.0% |
| ! | TGAATTTTGTGGTTGCGCCT+GGG | - | Chr1:35956324-35956343 | MsG0180002264.01.T01:CDS | 45.0% |
| ATCGGAGTCAAAGGAGAGGA+TGG | - | Chr1:35956271-35956290 | MsG0180002264.01.T01:CDS | 50.0% | |
| GAGGTGATGATATGGGAGGT+TGG | - | Chr1:35956359-35956378 | MsG0180002264.01.T01:CDS | 50.0% | |
| TGACATCGGAGTCAAAGGAG+AGG | - | Chr1:35956267-35956286 | MsG0180002264.01.T01:CDS | 50.0% | |
| TGCTGAGGTGATGATATGGG+AGG | - | Chr1:35956355-35956374 | MsG0180002264.01.T01:CDS | 50.0% | |
| ! | GCCTGGGAACTATTTTGCTG+AGG | - | Chr1:35956340-35956359 | MsG0180002264.01.T01:CDS | 50.0% |
| !! | AAAGGAGAGGATGGTGTTGC+AGG | - | Chr1:35956280-35956299 | MsG0180002264.01.T01:CDS | 50.0% |
| !! | AAGGAGAGGATGGTGTTGCA+GGG | - | Chr1:35956281-35956300 | MsG0180002264.01.T01:CDS | 50.0% |
| TGGGCTTGTGCCTGAACATG+TGG | - | Chr1:35956379-35956398 | MsG0180002264.01.T01:CDS | 55.0% | |
| !! | AGGAGAGGATGGTGTTGCAG+GGG | - | Chr1:35956282-35956301 | MsG0180002264.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 35956266 | 35956517 | 35956266 | ID=MsG0180002264.01;Name=MsG0180002264.01 |
| Chr1 | mRNA | 35956266 | 35956517 | 35956266 | ID=MsG0180002264.01.T01;Parent=MsG0180002264.01;Name=MsG0180002264.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|83 |
| Chr1 | exon | 35956266 | 35956517 | 35956266 | ID=MsG0180002264.01.T01:exon:39576;Parent=MsG0180002264.01.T01 |
| Chr1 | CDS | 35956266 | 35956517 | 35956266 | ID=MsG0180002264.01.T01:cds;Parent=MsG0180002264.01.T01 |
| Gene Sequence |
| Protein sequence |
