Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019161.01.T01 | XP_013447253.1 | 95.21 | 167 | 8 | 0 | 1 | 167 | 1 | 167 | 2.64E-110 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019161.01.T01 | Q9C723 | 76.647 | 167 | 39 | 0 | 1 | 167 | 1 | 167 | 2.18E-96 | 297 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019161.01.T01 | A0A072U616 | 95.210 | 167 | 8 | 0 | 1 | 167 | 1 | 167 | 1.26e-110 | 340 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000318.01 | MsG0480019161.01 | 0.818535 | 1.863445e-52 | 1.398624e-49 |
| MsG0180001706.01 | MsG0480019161.01 | 0.816758 | 4.688810e-52 | 3.351666e-49 |
| MsG0180002264.01 | MsG0480019161.01 | 0.817326 | 3.494528e-52 | 2.537085e-49 |
| MsG0180004335.01 | MsG0480019161.01 | 0.811449 | 6.961746e-51 | 4.310739e-48 |
| MsG0280010884.01 | MsG0480019161.01 | 0.846161 | 2.584063e-59 | 4.407694e-56 |
| MsG0380013794.01 | MsG0480019161.01 | 0.807631 | 4.600686e-50 | 2.575331e-47 |
| MsG0480019161.01 | MsG0480019162.01 | 0.977058 | 5.002003e-143 | 8.719228e-137 |
| MsG0480019161.01 | MsG0480019163.01 | 0.891823 | 2.794085e-74 | 2.488695e-70 |
| MsG0480019161.01 | MsG0480021490.01 | 0.821422 | 4.072897e-53 | 3.312090e-50 |
| MsG0480019161.01 | MsG0480022939.01 | 0.802866 | 4.577907e-49 | 2.267086e-46 |
| MsG0480019161.01 | MsG0480023726.01 | -0.803769 | 2.976135e-49 | 1.508353e-46 |
| MsG0480019161.01 | MsG0480023973.01 | 0.869341 | 3.328537e-66 | 1.244935e-62 |
| MsG0480019161.01 | MsG0780041470.01 | 0.813604 | 2.353032e-51 | 1.543596e-48 |
| MsG0480019161.01 | MsG0880043027.01 | 0.802215 | 6.236122e-49 | 3.037372e-46 |
| MsG0480019161.01 | MsG0880047179.01 | 0.827211 | 1.772894e-54 | 1.700323e-51 |
| MsG0280007111.01 | MsG0480019161.01 | 0.802901 | 4.501757e-49 | 2.231390e-46 |
| MsG0280009344.01 | MsG0480019161.01 | 0.804404 | 2.195891e-49 | 1.131167e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019161.01.T01 | MTR_8g099885 | 95.210 | 167 | 8 | 0 | 1 | 167 | 1 | 167 | 3.19e-114 | 340 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019161.01.T01 | AT1G55090 | 76.647 | 167 | 39 | 0 | 1 | 167 | 1 | 167 | 2.22e-97 | 297 |
Find 45 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTTATTACGAATGGTTAA+TGG | 0.329703 | 4:+15236752 | None:intergenic |
| GGAATGTTTGAAAGATATAT+TGG | 0.334800 | 4:-15236647 | MsG0480019161.01.T01:CDS |
| GGTTAATGGTGTCGAGTTCT+AGG | 0.340380 | 4:+15236766 | None:intergenic |
| GTCTTTGATTTGTTGAAGAT+TGG | 0.361625 | 4:+15236875 | None:intergenic |
| ATCATTTGCAAGCCACATCT+TGG | 0.378781 | 4:+15236510 | None:intergenic |
| CTCTCAGAACCTTTGATAAT+AGG | 0.381396 | 4:+15236583 | None:intergenic |
| AAGATATATTGGTTGGTGAT+TGG | 0.409668 | 4:-15236636 | MsG0480019161.01.T01:CDS |
| ATAACCGGGGATTTCGAGTT+CGG | 0.410395 | 4:+15236806 | None:intergenic |
| GTGGTCTTCACAGACATAAC+CGG | 0.410799 | 4:+15236791 | None:intergenic |
| AACTTCGATGGTTTACTGCT+TGG | 0.416004 | 4:-15236474 | MsG0480019161.01.T01:CDS |
| GTCGTATCATAATAATCTTT+CGG | 0.425212 | 4:+15236533 | None:intergenic |
| TCCTAATGTTTCCGAGGCAT+TGG | 0.428289 | 4:-15236416 | MsG0480019161.01.T01:CDS |
| GCCGGCGTCGTCGTCCGTCT+TGG | 0.458553 | 4:-15236831 | MsG0480019161.01.T01:CDS |
| CAATCTTAACCAATGGGCTA+TGG | 0.462534 | 4:-15236907 | MsG0480019161.01.T01:CDS |
| GATAACATAGTGTGCAGTAT+TGG | 0.463634 | 4:-15236610 | MsG0480019161.01.T01:CDS |
| GGCTTGTTTAGATTTGTAGA+TGG | 0.463676 | 4:+15236851 | None:intergenic |
| TTCTGAGAGATACAATTGTC+AGG | 0.465019 | 4:-15236569 | MsG0480019161.01.T01:CDS |
| TCATTTGCAAGCCACATCTT+GGG | 0.476765 | 4:+15236511 | None:intergenic |
| GCCAATGCCTCGGAAACATT+AGG | 0.480001 | 4:+15236415 | None:intergenic |
| TAACCGGGGATTTCGAGTTC+GGG | 0.480511 | 4:+15236807 | None:intergenic |
| TACTTGCAATCTTAACCAAT+GGG | 0.505809 | 4:-15236913 | MsG0480019161.01.T01:CDS |
| AATCACGATGAGGCTCTTGA+AGG | 0.512546 | 4:-15236940 | None:intergenic |
| GTTTACTGCTTGGAAACAAA+GGG | 0.526848 | 4:-15236464 | MsG0480019161.01.T01:CDS |
| TGGTGTCGAGTTCTAGGAAG+TGG | 0.535115 | 4:+15236772 | None:intergenic |
| TTAACAAAACCATAACCAAA+AGG | 0.535794 | 4:+15236379 | None:intergenic |
| CTACTTGCAATCTTAACCAA+TGG | 0.542643 | 4:-15236914 | MsG0480019161.01.T01:CDS |
| ATTGGAATGCCTATTATCAA+AGG | 0.545628 | 4:-15236592 | MsG0480019161.01.T01:CDS |
| AATCGAATTCCATAGCCCAT+TGG | 0.548373 | 4:+15236898 | None:intergenic |
| GGTTTACTGCTTGGAAACAA+AGG | 0.548451 | 4:-15236465 | MsG0480019161.01.T01:CDS |
| ACCAAGACGGACGACGACGC+CGG | 0.556438 | 4:+15236830 | None:intergenic |
| GAAGTTGAAAATCATCAAGT+TGG | 0.562415 | 4:+15236440 | None:intergenic |
| TGGTCTTCACAGACATAACC+GGG | 0.571329 | 4:+15236792 | None:intergenic |
| GTAATTATAGAGAACTTCGA+TGG | 0.573265 | 4:-15236486 | MsG0480019161.01.T01:CDS |
| ATCTACAAATCTAAACAAGC+CGG | 0.574227 | 4:-15236849 | MsG0480019161.01.T01:CDS |
| TGTTTGAAAGATATATTGGT+TGG | 0.589747 | 4:-15236643 | MsG0480019161.01.T01:CDS |
| AAGATGTGGCTTGCAAATGA+TGG | 0.599672 | 4:-15236508 | MsG0480019161.01.T01:CDS |
| ACTTCCTCCTAATGTTTCCG+AGG | 0.602942 | 4:-15236422 | MsG0480019161.01.T01:CDS |
| CAGTTACACTGACACGAACA+CGG | 0.617973 | 4:-15236286 | MsG0480019161.01.T01:CDS |
| TTATGATACGACCCAAGATG+TGG | 0.618671 | 4:-15236522 | MsG0480019161.01.T01:CDS |
| GGTCCCGAACTCGAAATCCC+CGG | 0.647848 | 4:-15236810 | MsG0480019161.01.T01:CDS |
| AGTTACACTGACACGAACAC+GGG | 0.650651 | 4:-15236285 | MsG0480019161.01.T01:CDS |
| AATGCCTCGGAAACATTAGG+AGG | 0.652440 | 4:+15236418 | None:intergenic |
| TTTCGAGTTCGGGACCAAGA+CGG | 0.655937 | 4:+15236817 | None:intergenic |
| CATTACTGATGTCTACACGT+CGG | 0.740844 | 4:+15236254 | None:intergenic |
| GGTCTTCACAGACATAACCG+GGG | 0.797753 | 4:+15236793 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATAAAGTGTGAATT+TGG | + | Chr4:15236865-15236884 | None:intergenic | 20.0% |
| ! | AAAGAAAGAGTTATTACGAA+TGG | + | Chr4:15236457-15236476 | None:intergenic | 25.0% |
| ! | GGAATGTTTGAAAGATATAT+TGG | - | Chr4:15236552-15236571 | MsG0480019161.01.T01:CDS | 25.0% |
| ! | GTCGTATCATAATAATCTTT+CGG | + | Chr4:15236669-15236688 | None:intergenic | 25.0% |
| ! | TGTTTGAAAGATATATTGGT+TGG | - | Chr4:15236556-15236575 | MsG0480019161.01.T01:CDS | 25.0% |
| ! | TTAACAAAACCATAACCAAA+AGG | + | Chr4:15236823-15236842 | None:intergenic | 25.0% |
| !!! | TTTGTTTTTGTCACTGAAAT+TGG | - | Chr4:15236512-15236531 | MsG0480019161.01.T01:CDS | 25.0% |
| ATCTACAAATCTAAACAAGC+CGG | - | Chr4:15236350-15236369 | MsG0480019161.01.T01:intron | 30.0% | |
| ATTGGAATGCCTATTATCAA+AGG | - | Chr4:15236607-15236626 | MsG0480019161.01.T01:CDS | 30.0% | |
| GAAGTTGAAAATCATCAAGT+TGG | + | Chr4:15236762-15236781 | None:intergenic | 30.0% | |
| GAGTTATTACGAATGGTTAA+TGG | + | Chr4:15236450-15236469 | None:intergenic | 30.0% | |
| GTAATTATAGAGAACTTCGA+TGG | - | Chr4:15236713-15236732 | MsG0480019161.01.T01:intron | 30.0% | |
| TACTTGCAATCTTAACCAAT+GGG | - | Chr4:15236286-15236305 | MsG0480019161.01.T01:CDS | 30.0% | |
| !! | AAATCAGTTCCTTTTGGTTA+TGG | - | Chr4:15236811-15236830 | MsG0480019161.01.T01:CDS | 30.0% |
| !! | AAGATATATTGGTTGGTGAT+TGG | - | Chr4:15236563-15236582 | MsG0480019161.01.T01:CDS | 30.0% |
| !! | GTCTTTGATTTGTTGAAGAT+TGG | + | Chr4:15236327-15236346 | None:intergenic | 30.0% |
| CTACTTGCAATCTTAACCAA+TGG | - | Chr4:15236285-15236304 | MsG0480019161.01.T01:CDS | 35.0% | |
| GATAACATAGTGTGCAGTAT+TGG | - | Chr4:15236589-15236608 | MsG0480019161.01.T01:CDS | 35.0% | |
| GTTTACTGCTTGGAAACAAA+GGG | - | Chr4:15236735-15236754 | MsG0480019161.01.T01:intron | 35.0% | |
| ! | CTCTCAGAACCTTTGATAAT+AGG | + | Chr4:15236619-15236638 | None:intergenic | 35.0% |
| ! | GCACAAAAATCAGTTCCTTT+TGG | - | Chr4:15236805-15236824 | MsG0480019161.01.T01:CDS | 35.0% |
| ! | GGCTTGTTTAGATTTGTAGA+TGG | + | Chr4:15236351-15236370 | None:intergenic | 35.0% |
| ! | TTCTGAGAGATACAATTGTC+AGG | - | Chr4:15236630-15236649 | MsG0480019161.01.T01:CDS | 35.0% |
| AACTTCGATGGTTTACTGCT+TGG | - | Chr4:15236725-15236744 | MsG0480019161.01.T01:intron | 40.0% | |
| AATCGAATTCCATAGCCCAT+TGG | + | Chr4:15236304-15236323 | None:intergenic | 40.0% | |
| ATCATTTGCAAGCCACATCT+TGG | + | Chr4:15236692-15236711 | None:intergenic | 40.0% | |
| GGTTTACTGCTTGGAAACAA+AGG | - | Chr4:15236734-15236753 | MsG0480019161.01.T01:intron | 40.0% | |
| TCATTTGCAAGCCACATCTT+GGG | + | Chr4:15236691-15236710 | None:intergenic | 40.0% | |
| TTATGATACGACCCAAGATG+TGG | - | Chr4:15236677-15236696 | MsG0480019161.01.T01:intron | 40.0% | |
| ! | AAGATGTGGCTTGCAAATGA+TGG | - | Chr4:15236691-15236710 | MsG0480019161.01.T01:intron | 40.0% |
| ! | TGATTTTTGTGCCAATGCCT+CGG | + | Chr4:15236797-15236816 | None:intergenic | 40.0% |
| !! | CAATCTTAACCAATGGGCTA+TGG | - | Chr4:15236292-15236311 | MsG0480019161.01.T01:intron | 40.0% |
| !!! | ATTGGTTTTGTAGCTGCAGA+TGG | - | Chr4:15236530-15236549 | MsG0480019161.01.T01:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCTGCAGAT+GGG | - | Chr4:15236531-15236550 | MsG0480019161.01.T01:CDS | 40.0% |
| AATGCCTCGGAAACATTAGG+AGG | + | Chr4:15236784-15236803 | None:intergenic | 45.0% | |
| ACTTCCTCCTAATGTTTCCG+AGG | - | Chr4:15236777-15236796 | MsG0480019161.01.T01:CDS | 45.0% | |
| AGTTACACTGACACGAACAC+GGG | - | Chr4:15236914-15236933 | MsG0480019161.01.T01:CDS | 45.0% | |
| CAGTTACACTGACACGAACA+CGG | - | Chr4:15236913-15236932 | MsG0480019161.01.T01:CDS | 45.0% | |
| GTGGTCTTCACAGACATAAC+CGG | + | Chr4:15236411-15236430 | None:intergenic | 45.0% | |
| TCCTAATGTTTCCGAGGCAT+TGG | - | Chr4:15236783-15236802 | MsG0480019161.01.T01:CDS | 45.0% | |
| TGGTCTTCACAGACATAACC+GGG | + | Chr4:15236410-15236429 | None:intergenic | 45.0% | |
| ! | GGTTAATGGTGTCGAGTTCT+AGG | + | Chr4:15236436-15236455 | None:intergenic | 45.0% |
| !! | ATAACCGGGGATTTCGAGTT+CGG | + | Chr4:15236396-15236415 | None:intergenic | 45.0% |
| GCCAATGCCTCGGAAACATT+AGG | + | Chr4:15236787-15236806 | None:intergenic | 50.0% | |
| GGTCTTCACAGACATAACCG+GGG | + | Chr4:15236409-15236428 | None:intergenic | 50.0% | |
| !! | TAACCGGGGATTTCGAGTTC+GGG | + | Chr4:15236395-15236414 | None:intergenic | 50.0% |
| !! | TGGTGTCGAGTTCTAGGAAG+TGG | + | Chr4:15236430-15236449 | None:intergenic | 50.0% |
| !! | TTTCGAGTTCGGGACCAAGA+CGG | + | Chr4:15236385-15236404 | None:intergenic | 50.0% |
| GGTCCCGAACTCGAAATCCC+CGG | - | Chr4:15236389-15236408 | MsG0480019161.01.T01:CDS | 60.0% | |
| ACCAAGACGGACGACGACGC+CGG | + | Chr4:15236372-15236391 | None:intergenic | 65.0% | |
| GCCGGCGTCGTCGTCCGTCT+TGG | - | Chr4:15236368-15236387 | MsG0480019161.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 15236266 | 15236955 | 15236266 | ID=MsG0480019161.01;Name=MsG0480019161.01 |
| Chr4 | mRNA | 15236266 | 15236955 | 15236266 | ID=MsG0480019161.01.T01;Parent=MsG0480019161.01;Name=MsG0480019161.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|0.5|0.66|3|0|179 |
| Chr4 | exon | 15236762 | 15236955 | 15236762 | ID=MsG0480019161.01.T01:exon:4943;Parent=MsG0480019161.01.T01 |
| Chr4 | exon | 15236367 | 15236672 | 15236367 | ID=MsG0480019161.01.T01:exon:4942;Parent=MsG0480019161.01.T01 |
| Chr4 | exon | 15236266 | 15236305 | 15236266 | ID=MsG0480019161.01.T01:exon:4941;Parent=MsG0480019161.01.T01 |
| Chr4 | CDS | 15236762 | 15236955 | 15236762 | ID=MsG0480019161.01.T01:cds;Parent=MsG0480019161.01.T01 |
| Chr4 | CDS | 15236367 | 15236672 | 15236367 | ID=MsG0480019161.01.T01:cds;Parent=MsG0480019161.01.T01 |
| Chr4 | CDS | 15236266 | 15236305 | 15236266 | ID=MsG0480019161.01.T01:cds;Parent=MsG0480019161.01.T01 |
| Gene Sequence |
| Protein sequence |
