Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002281.01.T01 | XP_013443255.1 | 83.784 | 111 | 14 | 1 | 1 | 111 | 1 | 107 | 2.32E-57 | 186 |
| MsG0180002281.01.T02 | XP_013443255.1 | 82.796 | 93 | 12 | 1 | 1 | 93 | 19 | 107 | 5.53E-46 | 156 |
| MsG0180002281.01.T03 | GAU40157.1 | 75.41 | 61 | 11 | 1 | 1 | 61 | 51 | 107 | 1.35E-18 | 85.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002281.01.T01 | Q39065 | 37.805 | 82 | 47 | 1 | 30 | 111 | 45 | 122 | 1.72E-11 | 62 |
| MsG0180002281.01.T02 | Q39065 | 34.831 | 89 | 54 | 1 | 12 | 100 | 45 | 129 | 1.20E-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002281.01.T01 | A0A072TUQ5 | 83.784 | 111 | 14 | 1 | 1 | 111 | 1 | 107 | 1.11e-57 | 186 |
| MsG0180002281.01.T02 | A0A072TUQ5 | 82.796 | 93 | 12 | 1 | 1 | 93 | 19 | 107 | 2.64e-46 | 156 |
| MsG0180002281.01.T03 | A0A2Z6PAW0 | 75.410 | 61 | 11 | 1 | 1 | 61 | 51 | 107 | 6.47e-19 | 85.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002281.01 | MsG0180003905.01 | 0.809768 | 1.607559e-50 | 9.521927e-48 |
| MsG0180002281.01 | MsG0180004102.01 | 0.811906 | 5.538482e-51 | 3.470949e-48 |
| MsG0180002281.01 | MsG0180004120.01 | 0.819076 | 1.404324e-52 | 1.069843e-49 |
| MsG0180002281.01 | MsG0280006924.01 | 0.841503 | 4.567027e-58 | 6.724488e-55 |
| MsG0180002281.01 | MsG0380015340.01 | 0.804021 | 2.638024e-49 | 1.345724e-46 |
| MsG0180002281.01 | MsG0380017588.01 | 0.800410 | 1.460214e-48 | 6.794490e-46 |
| MsG0180002281.01 | MsG0480018228.01 | 0.823669 | 1.222900e-53 | 1.059952e-50 |
| MsG0180002281.01 | MsG0480020366.01 | 0.808911 | 2.453632e-50 | 1.420400e-47 |
| MsG0180002281.01 | MsG0480020937.01 | 0.810601 | 1.062812e-50 | 6.434439e-48 |
| MsG0180002281.01 | MsG0480021773.01 | 0.806329 | 8.671843e-50 | 4.693827e-47 |
| MsG0180002281.01 | MsG0480022378.01 | 0.811348 | 7.322000e-51 | 4.521340e-48 |
| MsG0180002281.01 | MsG0680031356.01 | 0.812582 | 3.942878e-51 | 2.516518e-48 |
| MsG0180002281.01 | MsG0680033645.01 | 0.811944 | 5.434755e-51 | 3.409532e-48 |
| MsG0180002281.01 | MsG0780038952.01 | 0.863675 | 2.101251e-64 | 6.423665e-61 |
| MsG0180002281.01 | MsG0880043920.01 | 0.825778 | 3.892968e-54 | 3.583169e-51 |
| MsG0180002281.01 | MsG0880045356.01 | 0.823774 | 1.155645e-53 | 1.004674e-50 |
| MsG0180002281.01 | MsG0880046940.01 | 0.804929 | 1.706121e-49 | 8.909609e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002281.01.T01 | MTR_0027s0220 | 83.784 | 111 | 14 | 1 | 1 | 111 | 1 | 107 | 2.80e-61 | 186 |
| MsG0180002281.01.T01 | MTR_4g065660 | 41.860 | 86 | 43 | 4 | 30 | 115 | 35 | 113 | 4.86e-11 | 57.8 |
| MsG0180002281.01.T01 | MTR_4g064963 | 50.794 | 63 | 28 | 2 | 30 | 92 | 46 | 105 | 5.61e-11 | 57.8 |
| MsG0180002281.01.T02 | MTR_0027s0220 | 82.796 | 93 | 12 | 1 | 1 | 93 | 19 | 107 | 6.70e-50 | 156 |
| MsG0180002281.01.T02 | MTR_4g064963 | 50.794 | 63 | 28 | 2 | 12 | 74 | 46 | 105 | 3.24e-11 | 57.8 |
| MsG0180002281.01.T02 | MTR_4g065660 | 41.860 | 86 | 43 | 4 | 12 | 97 | 35 | 113 | 4.15e-11 | 57.4 |
| MsG0180002281.01.T03 | MTR_0027s0220 | 73.770 | 61 | 12 | 1 | 1 | 61 | 51 | 107 | 7.47e-22 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002281.01.T01 | AT2G26975 | 36.000 | 100 | 58 | 2 | 12 | 111 | 10 | 103 | 8.61e-13 | 62.0 |
| MsG0180002281.01.T01 | AT5G59030 | 37.805 | 82 | 47 | 1 | 30 | 111 | 45 | 122 | 1.76e-12 | 62.0 |
| MsG0180002281.01.T01 | AT3G46900 | 38.272 | 81 | 44 | 3 | 31 | 111 | 34 | 108 | 3.66e-11 | 58.2 |
| MsG0180002281.01.T02 | AT5G59030 | 34.831 | 89 | 54 | 1 | 12 | 100 | 45 | 129 | 1.23e-12 | 61.6 |
| MsG0180002281.01.T02 | AT2G26975 | 39.080 | 87 | 47 | 2 | 7 | 93 | 23 | 103 | 1.55e-12 | 60.8 |
| MsG0180002281.01.T02 | AT3G46900 | 32.353 | 102 | 63 | 3 | 13 | 114 | 34 | 129 | 1.96e-11 | 58.2 |
Find 47 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCCATGTTATTGGGTTTA+TGG | 0.163011 | 1:-36197398 | MsG0180002281.01.T01:CDS |
| GCAGTTGGTGGCCATGTTAT+TGG | 0.250916 | 1:-36197406 | MsG0180002281.01.T01:CDS |
| CGATCCACACATCATTCTAT+TGG | 0.284405 | 1:-36197685 | MsG0180002281.01.T01:CDS |
| CAGTTGGTGGCCATGTTATT+GGG | 0.316028 | 1:-36197405 | MsG0180002281.01.T01:CDS |
| GATCCACACATCATTCTATT+GGG | 0.329197 | 1:-36197684 | MsG0180002281.01.T01:CDS |
| TCCACGGTGGCGACGATGTT+GGG | 0.338831 | 1:-36197722 | MsG0180002281.01.T01:CDS |
| CCGTACTGGATTCTCGTACT+TGG | 0.410577 | 1:-36197472 | MsG0180002281.01.T01:CDS |
| TACTATTCCATTGCTGGCCC+GGG | 0.418443 | 1:-36197646 | MsG0180002281.01.T01:CDS |
| TAGACATACTATTCCATTGC+TGG | 0.428991 | 1:-36197652 | MsG0180002281.01.T01:CDS |
| GGGCTGTACGGTGTCCGTAC+TGG | 0.435927 | 1:-36197486 | MsG0180002281.01.T01:CDS |
| CTCCACGGTGGCGACGATGT+TGG | 0.436829 | 1:-36197723 | MsG0180002281.01.T01:CDS |
| ATACTATTCCATTGCTGGCC+CGG | 0.443048 | 1:-36197647 | MsG0180002281.01.T01:CDS |
| CGCTGTCCCCGGGCCAGCAA+TGG | 0.456744 | 1:+36197639 | None:intergenic |
| TTTGAAGAAATCTGGTGGGT+TGG | 0.457495 | 1:-36197356 | MsG0180002281.01.T01:CDS |
| CATCGTCGCCACCGTGGAGT+TGG | 0.477324 | 1:+36197727 | None:intergenic |
| GTACATGACAGCGCTGTCCC+CGG | 0.489328 | 1:+36197628 | None:intergenic |
| ATTCTCGTACTTGGTTATGT+TGG | 0.491065 | 1:-36197463 | MsG0180002281.01.T01:CDS |
| AGGTTTGAGTTTAACAATGT+TGG | 0.499385 | 1:+36197547 | None:intergenic |
| GTTTGTAATGTCGGTGTTGG+TGG | 0.507829 | 1:-36197585 | MsG0180002281.01.T01:CDS |
| ACGACGTCGTTTGATGTCCC+AGG | 0.512723 | 1:+36197527 | None:intergenic |
| TTGTTCACCACCACTGCAAA+TGG | 0.515915 | 1:-36197758 | MsG0180002281.01.T01:CDS |
| AACATTGTTAAACTCAAACC+TGG | 0.522755 | 1:-36197545 | MsG0180002281.01.T01:CDS |
| CGTGTTTGTAATGTCGGTGT+TGG | 0.532961 | 1:-36197588 | MsG0180002281.01.T01:CDS |
| GTGTTTCTAGCTGCAGTTGG+TGG | 0.544075 | 1:-36197418 | MsG0180002281.01.T01:CDS |
| TACATGACAGCGCTGTCCCC+GGG | 0.550555 | 1:+36197629 | None:intergenic |
| CGGTGGCGACGATGTTGGGC+CGG | 0.564945 | 1:-36197718 | MsG0180002281.01.T01:CDS |
| TGTGGATCGGAATTCTTCGC+CGG | 0.568160 | 1:+36197699 | None:intergenic |
| TGTGTGTTTCTAGCTGCAGT+TGG | 0.576611 | 1:-36197421 | MsG0180002281.01.T01:CDS |
| GATCCTCGTGTTTGTAATGT+CGG | 0.579313 | 1:-36197594 | MsG0180002281.01.T01:CDS |
| TAATGTCGGTGTTGGTGGAG+TGG | 0.582279 | 1:-36197580 | MsG0180002281.01.T01:CDS |
| CAATAGAATGATGTGTGGAT+CGG | 0.613976 | 1:+36197686 | None:intergenic |
| AGGAACACCATTTGCAGTGG+TGG | 0.618142 | 1:+36197751 | None:intergenic |
| GTCGCCACCGTGGAGTTGGA+AGG | 0.621088 | 1:+36197731 | None:intergenic |
| ACATTGTTAAACTCAAACCT+GGG | 0.621137 | 1:-36197544 | MsG0180002281.01.T01:CDS |
| TGGTGTTCCTTCCAACTCCA+CGG | 0.621560 | 1:-36197738 | MsG0180002281.01.T01:CDS |
| GGGACATCAAACGACGTCGT+TGG | 0.623966 | 1:-36197524 | MsG0180002281.01.T01:CDS |
| GGAAGGAACACCATTTGCAG+TGG | 0.632915 | 1:+36197748 | None:intergenic |
| ACTATTCCATTGCTGGCCCG+GGG | 0.640157 | 1:-36197645 | MsG0180002281.01.T01:CDS |
| ATCCACACATCATTCTATTG+GGG | 0.654547 | 1:-36197683 | MsG0180002281.01.T01:CDS |
| AAACCATAAACCCAATAACA+TGG | 0.667055 | 1:+36197395 | None:intergenic |
| CAGCGCTGTCATGTACGCGG+TGG | 0.674127 | 1:-36197621 | MsG0180002281.01.T01:CDS |
| GACCCCAATAGAATGATGTG+TGG | 0.683603 | 1:+36197681 | None:intergenic |
| GCCCAACATCGTCGCCACCG+TGG | 0.693359 | 1:+36197721 | None:intergenic |
| CCAAGTACGAGAATCCAGTA+CGG | 0.693631 | 1:+36197472 | None:intergenic |
| GGACAGCGCTGTCATGTACG+CGG | 0.705704 | 1:-36197624 | MsG0180002281.01.T01:CDS |
| ACACCGACATTACAAACACG+AGG | 0.732297 | 1:+36197591 | None:intergenic |
| TGTTCCTTCCAACTCCACGG+TGG | 0.776714 | 1:-36197735 | MsG0180002281.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCATAAACCCAATAACA+TGG | + | Chr1:36197712-36197731 | None:intergenic | 30.0% | |
| AACATTGTTAAACTCAAACC+TGG | - | Chr1:36197559-36197578 | MsG0180002281.01.T01:CDS | 30.0% | |
| ACATTGTTAAACTCAAACCT+GGG | - | Chr1:36197560-36197579 | MsG0180002281.01.T01:CDS | 30.0% | |
| ! | AGGTTTGAGTTTAACAATGT+TGG | + | Chr1:36197560-36197579 | None:intergenic | 30.0% |
| ! | GTTATTGGGTTTATGGTTTT+TGG | - | Chr1:36197713-36197732 | MsG0180002281.01.T01:CDS | 30.0% |
| ! | TTGGCTGTTATGTCTTTTAA+TGG | - | Chr1:36197660-36197679 | MsG0180002281.01.T01:CDS | 30.0% |
| !! | ATGGCTATTTTGAAGAAATC+TGG | - | Chr1:36197740-36197759 | MsG0180002281.01.T01:CDS | 30.0% |
| !! | CTATTTTGAAGAAATCTGGT+GGG | - | Chr1:36197744-36197763 | MsG0180002281.01.T01:CDS | 30.0% |
| !!! | GTTTATGGTTTTTGGTACAA+TGG | - | Chr1:36197721-36197740 | MsG0180002281.01.T01:CDS | 30.0% |
| ATCCACACATCATTCTATTG+GGG | - | Chr1:36197421-36197440 | MsG0180002281.01.T01:CDS | 35.0% | |
| ATTCTCGTACTTGGTTATGT+TGG | - | Chr1:36197641-36197660 | MsG0180002281.01.T01:CDS | 35.0% | |
| CAATAGAATGATGTGTGGAT+CGG | + | Chr1:36197421-36197440 | None:intergenic | 35.0% | |
| GATCCACACATCATTCTATT+GGG | - | Chr1:36197420-36197439 | MsG0180002281.01.T01:CDS | 35.0% | |
| TAGACATACTATTCCATTGC+TGG | - | Chr1:36197452-36197471 | MsG0180002281.01.T01:CDS | 35.0% | |
| !! | GCTATTTTGAAGAAATCTGG+TGG | - | Chr1:36197743-36197762 | MsG0180002281.01.T01:CDS | 35.0% |
| CGATCCACACATCATTCTAT+TGG | - | Chr1:36197419-36197438 | MsG0180002281.01.T01:CDS | 40.0% | |
| GATCCTCGTGTTTGTAATGT+CGG | - | Chr1:36197510-36197529 | MsG0180002281.01.T01:CDS | 40.0% | |
| TGGCCATGTTATTGGGTTTA+TGG | - | Chr1:36197706-36197725 | MsG0180002281.01.T01:CDS | 40.0% | |
| ! | TTTGAAGAAATCTGGTGGGT+TGG | - | Chr1:36197748-36197767 | MsG0180002281.01.T01:CDS | 40.0% |
| !! | GTTGGCTCACTTTTAAGAAC+GGG | - | Chr1:36197598-36197617 | MsG0180002281.01.T01:CDS | 40.0% |
| !!! | ACTTTTAAGAACGGGCTGTA+CGG | - | Chr1:36197606-36197625 | MsG0180002281.01.T01:CDS | 40.0% |
| !!! | CGTTGGCTCACTTTTAAGAA+CGG | - | Chr1:36197597-36197616 | MsG0180002281.01.T01:CDS | 40.0% |
| ACACCGACATTACAAACACG+AGG | + | Chr1:36197516-36197535 | None:intergenic | 45.0% | |
| ATACTATTCCATTGCTGGCC+CGG | - | Chr1:36197457-36197476 | MsG0180002281.01.T01:CDS | 45.0% | |
| CCAAGTACGAGAATCCAGTA+CGG | + | Chr1:36197635-36197654 | None:intergenic | 45.0% | |
| CGTGTTTGTAATGTCGGTGT+TGG | - | Chr1:36197516-36197535 | MsG0180002281.01.T01:CDS | 45.0% | |
| GACCCCAATAGAATGATGTG+TGG | + | Chr1:36197426-36197445 | None:intergenic | 45.0% | |
| TTGTTCACCACCACTGCAAA+TGG | - | Chr1:36197346-36197365 | MsG0180002281.01.T01:CDS | 45.0% | |
| !! | CAGTTGGTGGCCATGTTATT+GGG | - | Chr1:36197699-36197718 | MsG0180002281.01.T01:CDS | 45.0% |
| !! | GTTTGTAATGTCGGTGTTGG+TGG | - | Chr1:36197519-36197538 | MsG0180002281.01.T01:CDS | 45.0% |
| !! | TGTGTGTTTCTAGCTGCAGT+TGG | - | Chr1:36197683-36197702 | MsG0180002281.01.T01:CDS | 45.0% |
| AGGAACACCATTTGCAGTGG+TGG | + | Chr1:36197356-36197375 | None:intergenic | 50.0% | |
| CCGTACTGGATTCTCGTACT+TGG | - | Chr1:36197632-36197651 | MsG0180002281.01.T01:CDS | 50.0% | |
| GGAAGGAACACCATTTGCAG+TGG | + | Chr1:36197359-36197378 | None:intergenic | 50.0% | |
| TACTATTCCATTGCTGGCCC+GGG | - | Chr1:36197458-36197477 | MsG0180002281.01.T01:CDS | 50.0% | |
| TGGTGTTCCTTCCAACTCCA+CGG | - | Chr1:36197366-36197385 | MsG0180002281.01.T01:CDS | 50.0% | |
| TGTGGATCGGAATTCTTCGC+CGG | + | Chr1:36197408-36197427 | None:intergenic | 50.0% | |
| !! | GCAGTTGGTGGCCATGTTAT+TGG | - | Chr1:36197698-36197717 | MsG0180002281.01.T01:CDS | 50.0% |
| !! | GTGTTTCTAGCTGCAGTTGG+TGG | - | Chr1:36197686-36197705 | MsG0180002281.01.T01:CDS | 50.0% |
| !! | TAATGTCGGTGTTGGTGGAG+TGG | - | Chr1:36197524-36197543 | MsG0180002281.01.T01:CDS | 50.0% |
| ACGACGTCGTTTGATGTCCC+AGG | + | Chr1:36197580-36197599 | None:intergenic | 55.0% | |
| ACTATTCCATTGCTGGCCCG+GGG | - | Chr1:36197459-36197478 | MsG0180002281.01.T01:CDS | 55.0% | |
| GGGACATCAAACGACGTCGT+TGG | - | Chr1:36197580-36197599 | MsG0180002281.01.T01:CDS | 55.0% | |
| TGTTCCTTCCAACTCCACGG+TGG | - | Chr1:36197369-36197388 | MsG0180002281.01.T01:CDS | 55.0% | |
| GGACAGCGCTGTCATGTACG+CGG | - | Chr1:36197480-36197499 | MsG0180002281.01.T01:CDS | 60.0% | |
| GTACATGACAGCGCTGTCCC+CGG | + | Chr1:36197479-36197498 | None:intergenic | 60.0% | |
| TACATGACAGCGCTGTCCCC+GGG | + | Chr1:36197478-36197497 | None:intergenic | 60.0% | |
| TCCACGGTGGCGACGATGTT+GGG | - | Chr1:36197382-36197401 | MsG0180002281.01.T01:CDS | 60.0% | |
| CAGCGCTGTCATGTACGCGG+TGG | - | Chr1:36197483-36197502 | MsG0180002281.01.T01:CDS | 65.0% | |
| CATCGTCGCCACCGTGGAGT+TGG | + | Chr1:36197380-36197399 | None:intergenic | 65.0% | |
| CTCCACGGTGGCGACGATGT+TGG | - | Chr1:36197381-36197400 | MsG0180002281.01.T01:CDS | 65.0% | |
| GGGCTGTACGGTGTCCGTAC+TGG | - | Chr1:36197618-36197637 | MsG0180002281.01.T01:CDS | 65.0% | |
| GTCGCCACCGTGGAGTTGGA+AGG | + | Chr1:36197376-36197395 | None:intergenic | 65.0% | |
| CGGTGGCGACGATGTTGGGC+CGG | - | Chr1:36197386-36197405 | MsG0180002281.01.T01:CDS | 70.0% | |
| GCCCAACATCGTCGCCACCG+TGG | + | Chr1:36197386-36197405 | None:intergenic | 70.0% | |
| CGCTGTCCCCGGGCCAGCAA+TGG | + | Chr1:36197468-36197487 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 36197343 | 36197783 | 36197343 | ID=MsG0180002281.01;Name=MsG0180002281.01 |
| Chr1 | mRNA | 36197343 | 36197783 | 36197343 | ID=MsG0180002281.01.T01;Parent=MsG0180002281.01;Name=MsG0180002281.01.T01;_AED=0.00;_eAED=0.20;_QI=0|-1|0|1|-1|0|1|0|146 |
| Chr1 | exon | 36197343 | 36197783 | 36197343 | ID=MsG0180002281.01.T01:exon:1175;Parent=MsG0180002281.01.T01 |
| Chr1 | CDS | 36197343 | 36197783 | 36197343 | ID=MsG0180002281.01.T01:cds;Parent=MsG0180002281.01.T01 |
| Chr1 | mRNA | 36197343 | 36197729 | 36197343 | ID=MsG0180002281.01.T02;Parent=MsG0180002281.01;Name=MsG0180002281.01.T02;_AED=0.07;_eAED=0.28;_QI=0|-1|0|1|-1|0|1|0|128 |
| Chr1 | exon | 36197343 | 36197729 | 36197343 | ID=MsG0180002281.01.T02:exon:1176;Parent=MsG0180002281.01.T02 |
| Chr1 | CDS | 36197343 | 36197729 | 36197343 | ID=MsG0180002281.01.T02:cds;Parent=MsG0180002281.01.T02 |
| Chr1 | mRNA | 36197343 | 36197633 | 36197343 | ID=MsG0180002281.01.T03;Parent=MsG0180002281.01;Name=MsG0180002281.01.T03;_AED=0.17;_eAED=0.42;_QI=0|-1|0|1|-1|0|1|0|96 |
| Chr1 | exon | 36197343 | 36197633 | 36197343 | ID=MsG0180002281.01.T03:exon:1177;Parent=MsG0180002281.01.T03 |
| Chr1 | CDS | 36197343 | 36197633 | 36197343 | ID=MsG0180002281.01.T03:cds;Parent=MsG0180002281.01.T03 |
| Gene Sequence |
| Protein sequence |
