Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003727.01.T01 | XP_024634883.1 | 95.833 | 264 | 11 | 0 | 1 | 264 | 1 | 264 | 2.40E-166 | 471 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003727.01.T01 | Q9SQR4 | 63.838 | 271 | 90 | 2 | 1 | 264 | 1 | 270 | 2.31E-122 | 352 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003727.01.T01 | A0A396JR15 | 95.833 | 264 | 11 | 0 | 1 | 264 | 1 | 264 | 1.15e-166 | 471 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003727.01.T01 | MTR_1g068955 | 95.785 | 261 | 11 | 0 | 1 | 261 | 1 | 261 | 1.21e-167 | 465 |
| MsG0180003727.01.T01 | MTR_7g094510 | 73.123 | 253 | 67 | 1 | 12 | 264 | 4 | 255 | 1.02e-135 | 383 |
| MsG0180003727.01.T01 | MTR_7g094520 | 69.444 | 252 | 76 | 1 | 13 | 264 | 10 | 260 | 9.33e-132 | 373 |
| MsG0180003727.01.T01 | MTR_3g085740 | 35.821 | 268 | 159 | 8 | 1 | 264 | 61 | 319 | 2.45e-35 | 129 |
| MsG0180003727.01.T01 | MTR_7g063260 | 31.538 | 260 | 157 | 6 | 14 | 263 | 16 | 264 | 6.96e-28 | 108 |
| MsG0180003727.01.T01 | MTR_7g063270 | 30.502 | 259 | 160 | 6 | 14 | 263 | 16 | 263 | 1.68e-27 | 107 |
| MsG0180003727.01.T01 | MTR_6g023600 | 30.970 | 268 | 173 | 7 | 1 | 263 | 1 | 261 | 2.91e-27 | 106 |
| MsG0180003727.01.T01 | MTR_6g023590 | 30.970 | 268 | 173 | 7 | 1 | 263 | 1 | 261 | 3.27e-27 | 106 |
| MsG0180003727.01.T01 | MTR_6g023600 | 30.970 | 268 | 167 | 7 | 1 | 263 | 1 | 255 | 5.75e-27 | 105 |
| MsG0180003727.01.T01 | MTR_7g063360 | 29.804 | 255 | 168 | 4 | 14 | 263 | 16 | 264 | 6.99e-26 | 102 |
| MsG0180003727.01.T01 | MTR_7g063320 | 29.688 | 256 | 169 | 5 | 14 | 264 | 15 | 264 | 1.03e-25 | 102 |
| MsG0180003727.01.T01 | MTR_7g071680 | 31.408 | 277 | 155 | 8 | 11 | 262 | 26 | 292 | 1.89e-25 | 102 |
| MsG0180003727.01.T01 | MTR_3g068175 | 28.458 | 253 | 168 | 6 | 14 | 262 | 17 | 260 | 2.59e-25 | 101 |
| MsG0180003727.01.T01 | MTR_6g023610 | 32.184 | 261 | 160 | 9 | 12 | 264 | 11 | 262 | 1.94e-24 | 99.0 |
| MsG0180003727.01.T01 | MTR_7g107670 | 31.034 | 261 | 159 | 5 | 14 | 263 | 33 | 283 | 3.34e-24 | 99.0 |
| MsG0180003727.01.T01 | MTR_3g068070 | 29.644 | 253 | 167 | 6 | 14 | 263 | 15 | 259 | 3.88e-24 | 98.2 |
| MsG0180003727.01.T01 | MTR_7g063390 | 29.020 | 255 | 170 | 4 | 14 | 263 | 16 | 264 | 4.61e-24 | 97.8 |
| MsG0180003727.01.T01 | MTR_6g023950 | 30.224 | 268 | 175 | 7 | 1 | 263 | 1 | 261 | 5.75e-24 | 97.8 |
| MsG0180003727.01.T01 | MTR_4g064887 | 30.859 | 256 | 158 | 8 | 14 | 262 | 15 | 258 | 7.25e-24 | 97.4 |
| MsG0180003727.01.T01 | MTR_3g068085 | 29.134 | 254 | 167 | 6 | 14 | 263 | 15 | 259 | 9.30e-24 | 97.4 |
| MsG0180003727.01.T01 | MTR_6g023910 | 29.730 | 259 | 171 | 6 | 11 | 264 | 10 | 262 | 2.78e-23 | 95.9 |
| MsG0180003727.01.T01 | MTR_3g068175 | 28.016 | 257 | 168 | 7 | 14 | 262 | 17 | 264 | 3.35e-23 | 95.9 |
| MsG0180003727.01.T01 | MTR_3g068065 | 28.571 | 252 | 171 | 7 | 14 | 263 | 17 | 261 | 4.63e-23 | 95.5 |
| MsG0180003727.01.T01 | MTR_6g023990 | 30.000 | 270 | 174 | 8 | 1 | 263 | 1 | 262 | 8.73e-23 | 94.4 |
| MsG0180003727.01.T01 | MTR_3g068155 | 29.528 | 254 | 164 | 7 | 14 | 262 | 19 | 262 | 1.99e-22 | 93.6 |
| MsG0180003727.01.T01 | MTR_1196s0010 | 29.921 | 254 | 158 | 6 | 16 | 264 | 276 | 514 | 2.16e-22 | 96.3 |
| MsG0180003727.01.T01 | MTR_7g105590 | 33.500 | 200 | 116 | 7 | 14 | 208 | 8 | 195 | 1.39e-21 | 89.7 |
| MsG0180003727.01.T01 | MTR_4g131740 | 28.000 | 275 | 179 | 6 | 6 | 264 | 20 | 291 | 1.77e-21 | 91.7 |
| MsG0180003727.01.T01 | MTR_7g083870 | 28.846 | 260 | 167 | 5 | 14 | 263 | 35 | 286 | 2.40e-21 | 91.3 |
| MsG0180003727.01.T01 | MTR_7g063330 | 27.344 | 256 | 172 | 6 | 14 | 263 | 15 | 262 | 3.76e-21 | 90.1 |
| MsG0180003727.01.T01 | MTR_7g105580 | 28.253 | 269 | 154 | 7 | 1 | 263 | 1 | 236 | 1.13e-20 | 88.6 |
| MsG0180003727.01.T01 | MTR_4g064883 | 29.200 | 250 | 170 | 5 | 14 | 262 | 14 | 257 | 1.16e-20 | 89.0 |
| MsG0180003727.01.T01 | MTR_8g442750 | 30.797 | 276 | 165 | 10 | 1 | 262 | 1 | 264 | 6.04e-20 | 87.4 |
| MsG0180003727.01.T01 | MTR_4g132850 | 29.804 | 255 | 165 | 7 | 11 | 262 | 4 | 247 | 6.51e-19 | 84.0 |
| MsG0180003727.01.T01 | MTR_4g078630 | 29.434 | 265 | 163 | 7 | 11 | 262 | 16 | 269 | 1.03e-18 | 84.0 |
| MsG0180003727.01.T01 | MTR_7g105460 | 29.528 | 254 | 168 | 4 | 14 | 263 | 13 | 259 | 1.15e-18 | 83.6 |
| MsG0180003727.01.T01 | MTR_3g020670 | 29.434 | 265 | 161 | 9 | 14 | 263 | 16 | 269 | 2.16e-18 | 82.8 |
| MsG0180003727.01.T01 | MTR_1g082760 | 28.777 | 278 | 172 | 10 | 6 | 264 | 20 | 290 | 3.14e-18 | 82.4 |
| MsG0180003727.01.T01 | MTR_4g052340 | 26.866 | 268 | 176 | 5 | 1 | 261 | 1 | 255 | 4.01e-18 | 82.0 |
| MsG0180003727.01.T01 | MTR_7g113650 | 27.863 | 262 | 167 | 9 | 14 | 264 | 40 | 290 | 7.05e-18 | 81.6 |
| MsG0180003727.01.T01 | MTR_7g063270 | 38.614 | 101 | 58 | 1 | 167 | 263 | 114 | 214 | 1.05e-16 | 77.0 |
| MsG0180003727.01.T01 | MTR_4g052300 | 28.968 | 252 | 167 | 5 | 14 | 262 | 8 | 250 | 5.26e-16 | 75.9 |
| MsG0180003727.01.T01 | MTR_4g052320 | 28.063 | 253 | 171 | 5 | 14 | 263 | 8 | 252 | 6.06e-16 | 75.9 |
| MsG0180003727.01.T01 | MTR_5g022330 | 50.000 | 112 | 11 | 2 | 108 | 219 | 14 | 80 | 1.09e-15 | 70.5 |
| MsG0180003727.01.T01 | MTR_4g058810 | 27.203 | 261 | 177 | 9 | 10 | 262 | 6 | 261 | 1.26e-15 | 75.1 |
| MsG0180003727.01.T01 | MTR_4g052350 | 27.413 | 259 | 162 | 6 | 14 | 262 | 14 | 256 | 1.53e-15 | 74.7 |
| MsG0180003727.01.T01 | MTR_7g080410 | 29.798 | 198 | 129 | 4 | 14 | 207 | 38 | 229 | 3.07e-15 | 74.7 |
| MsG0180003727.01.T01 | MTR_7g080410 | 29.798 | 198 | 129 | 4 | 14 | 207 | 38 | 229 | 3.68e-15 | 73.9 |
| MsG0180003727.01.T01 | MTR_4g058840 | 26.415 | 265 | 183 | 8 | 5 | 262 | 2 | 261 | 3.90e-15 | 73.6 |
| MsG0180003727.01.T01 | MTR_4g058820 | 26.415 | 265 | 183 | 8 | 5 | 262 | 2 | 261 | 3.90e-15 | 73.6 |
| MsG0180003727.01.T01 | MTR_1g051165 | 28.413 | 271 | 166 | 10 | 10 | 262 | 9 | 269 | 6.02e-15 | 73.2 |
| MsG0180003727.01.T01 | MTR_3g113870 | 28.986 | 207 | 137 | 4 | 22 | 228 | 29 | 225 | 7.99e-14 | 69.3 |
| MsG0180003727.01.T01 | MTR_7g110140 | 24.715 | 263 | 169 | 7 | 14 | 263 | 6 | 252 | 2.20e-12 | 65.9 |
| MsG0180003727.01.T01 | MTR_4g064887 | 29.208 | 202 | 125 | 7 | 14 | 209 | 15 | 204 | 4.27e-12 | 64.3 |
| MsG0180003727.01.T01 | MTR_8g096580 | 29.310 | 232 | 140 | 9 | 14 | 237 | 45 | 260 | 6.06e-12 | 65.1 |
| MsG0180003727.01.T01 | MTR_7g105570 | 34.132 | 167 | 106 | 2 | 101 | 263 | 50 | 216 | 9.17e-12 | 63.5 |
| MsG0180003727.01.T01 | MTR_7g110130 | 25.758 | 264 | 166 | 8 | 14 | 263 | 6 | 253 | 1.48e-11 | 63.5 |
| MsG0180003727.01.T01 | MTR_4g064883 | 28.000 | 200 | 138 | 4 | 10 | 209 | 10 | 203 | 5.88e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003727.01.T01 | AT3G03980 | 63.838 | 271 | 90 | 2 | 1 | 264 | 1 | 270 | 2.35e-123 | 352 |
| MsG0180003727.01.T01 | AT5G18210 | 64.961 | 254 | 85 | 2 | 14 | 264 | 8 | 260 | 2.75e-116 | 334 |
| MsG0180003727.01.T01 | AT4G13180 | 56.061 | 264 | 114 | 2 | 1 | 264 | 1 | 262 | 1.34e-110 | 320 |
| MsG0180003727.01.T01 | AT3G04000 | 57.407 | 270 | 106 | 2 | 1 | 262 | 1 | 269 | 1.98e-108 | 315 |
| MsG0180003727.01.T01 | AT5G18210 | 65.690 | 239 | 78 | 2 | 14 | 249 | 8 | 245 | 3.26e-107 | 311 |
| MsG0180003727.01.T01 | AT5G06060 | 31.076 | 251 | 166 | 5 | 14 | 263 | 9 | 253 | 1.63e-28 | 109 |
| MsG0180003727.01.T01 | AT1G24360 | 32.558 | 258 | 160 | 7 | 6 | 262 | 72 | 316 | 3.68e-28 | 110 |
| MsG0180003727.01.T01 | AT3G26760 | 30.153 | 262 | 163 | 7 | 14 | 263 | 36 | 289 | 2.16e-26 | 105 |
| MsG0180003727.01.T01 | AT3G51680 | 33.579 | 271 | 155 | 11 | 11 | 262 | 29 | 293 | 3.06e-25 | 102 |
| MsG0180003727.01.T01 | AT2G29260 | 28.740 | 254 | 174 | 5 | 10 | 262 | 64 | 311 | 4.51e-24 | 99.0 |
| MsG0180003727.01.T01 | AT3G46170 | 29.615 | 260 | 172 | 8 | 10 | 262 | 21 | 276 | 1.25e-23 | 97.4 |
| MsG0180003727.01.T01 | AT2G30670 | 29.762 | 252 | 166 | 6 | 14 | 262 | 7 | 250 | 3.77e-23 | 95.5 |
| MsG0180003727.01.T01 | AT2G47130 | 29.730 | 259 | 164 | 7 | 12 | 263 | 4 | 251 | 5.18e-23 | 95.1 |
| MsG0180003727.01.T01 | AT2G29150 | 29.200 | 250 | 169 | 6 | 14 | 262 | 16 | 258 | 6.96e-23 | 94.7 |
| MsG0180003727.01.T01 | AT1G62610 | 30.153 | 262 | 169 | 10 | 10 | 262 | 10 | 266 | 7.63e-23 | 95.1 |
| MsG0180003727.01.T01 | AT2G29360 | 30.081 | 246 | 165 | 6 | 19 | 263 | 21 | 260 | 8.13e-23 | 94.7 |
| MsG0180003727.01.T01 | AT3G55290 | 27.820 | 266 | 181 | 8 | 4 | 262 | 6 | 267 | 8.69e-23 | 94.7 |
| MsG0180003727.01.T01 | AT1G62610 | 30.153 | 262 | 169 | 10 | 10 | 262 | 9 | 265 | 8.84e-23 | 94.7 |
| MsG0180003727.01.T01 | AT2G29290 | 29.885 | 261 | 154 | 8 | 14 | 262 | 7 | 250 | 9.72e-23 | 94.4 |
| MsG0180003727.01.T01 | AT1G62610 | 30.153 | 262 | 169 | 10 | 10 | 262 | 14 | 270 | 1.04e-22 | 94.7 |
| MsG0180003727.01.T01 | AT3G55290 | 27.757 | 263 | 179 | 8 | 7 | 262 | 10 | 268 | 1.11e-22 | 94.7 |
| MsG0180003727.01.T01 | AT1G62610 | 30.153 | 262 | 169 | 10 | 10 | 262 | 8 | 264 | 1.11e-22 | 94.4 |
| MsG0180003727.01.T01 | AT2G17845 | 29.457 | 258 | 169 | 8 | 14 | 262 | 47 | 300 | 1.48e-22 | 94.7 |
| MsG0180003727.01.T01 | AT1G07440 | 29.719 | 249 | 169 | 4 | 14 | 262 | 12 | 254 | 4.14e-22 | 92.8 |
| MsG0180003727.01.T01 | AT3G29260 | 29.572 | 257 | 163 | 7 | 14 | 263 | 6 | 251 | 4.36e-22 | 92.4 |
| MsG0180003727.01.T01 | AT3G55310 | 28.462 | 260 | 175 | 8 | 10 | 262 | 12 | 267 | 7.32e-22 | 92.4 |
| MsG0180003727.01.T01 | AT1G63380 | 29.278 | 263 | 170 | 10 | 10 | 262 | 10 | 266 | 7.83e-22 | 92.4 |
| MsG0180003727.01.T01 | AT1G63380 | 29.278 | 263 | 170 | 10 | 10 | 262 | 12 | 268 | 7.93e-22 | 92.4 |
| MsG0180003727.01.T01 | AT1G63380 | 29.278 | 263 | 170 | 10 | 10 | 262 | 14 | 270 | 8.02e-22 | 92.4 |
| MsG0180003727.01.T01 | AT1G63380 | 29.278 | 263 | 170 | 10 | 10 | 262 | 17 | 273 | 9.31e-22 | 92.0 |
| MsG0180003727.01.T01 | AT2G29350 | 27.869 | 244 | 170 | 3 | 19 | 262 | 20 | 257 | 2.01e-21 | 90.9 |
| MsG0180003727.01.T01 | AT3G29250 | 28.462 | 260 | 167 | 8 | 12 | 263 | 4 | 252 | 5.42e-21 | 89.7 |
| MsG0180003727.01.T01 | AT3G29250 | 27.820 | 266 | 173 | 8 | 6 | 263 | 34 | 288 | 7.29e-21 | 90.1 |
| MsG0180003727.01.T01 | AT2G29370 | 30.315 | 254 | 164 | 8 | 14 | 263 | 16 | 260 | 9.34e-21 | 89.0 |
| MsG0180003727.01.T01 | AT1G07450 | 29.921 | 254 | 164 | 7 | 14 | 263 | 8 | 251 | 1.56e-20 | 88.6 |
| MsG0180003727.01.T01 | AT1G52340 | 31.579 | 266 | 157 | 7 | 14 | 263 | 18 | 274 | 1.76e-20 | 88.6 |
| MsG0180003727.01.T01 | AT3G29250 | 28.295 | 258 | 166 | 8 | 14 | 263 | 44 | 290 | 2.26e-20 | 88.6 |
| MsG0180003727.01.T01 | AT2G47120 | 27.344 | 256 | 173 | 6 | 12 | 263 | 4 | 250 | 3.17e-20 | 87.4 |
| MsG0180003727.01.T01 | AT2G29290 | 31.217 | 189 | 125 | 5 | 76 | 262 | 48 | 233 | 4.87e-20 | 86.7 |
| MsG0180003727.01.T01 | AT2G29310 | 30.040 | 253 | 166 | 6 | 14 | 263 | 7 | 251 | 7.06e-20 | 86.7 |
| MsG0180003727.01.T01 | AT2G47120 | 27.165 | 254 | 172 | 6 | 14 | 263 | 7 | 251 | 1.03e-19 | 86.3 |
| MsG0180003727.01.T01 | AT3G05260 | 27.437 | 277 | 176 | 9 | 6 | 264 | 17 | 286 | 1.17e-19 | 86.7 |
| MsG0180003727.01.T01 | AT2G29350 | 29.412 | 187 | 130 | 1 | 76 | 262 | 35 | 219 | 2.31e-19 | 84.7 |
| MsG0180003727.01.T01 | AT4G03140 | 29.104 | 268 | 155 | 8 | 14 | 263 | 78 | 328 | 5.42e-19 | 85.5 |
| MsG0180003727.01.T01 | AT2G29330 | 29.200 | 250 | 170 | 5 | 14 | 262 | 7 | 250 | 6.14e-19 | 84.0 |
| MsG0180003727.01.T01 | AT1G54870 | 29.119 | 261 | 165 | 8 | 14 | 264 | 82 | 332 | 9.15e-18 | 82.0 |
| MsG0180003727.01.T01 | AT1G54870 | 29.119 | 261 | 165 | 8 | 14 | 264 | 84 | 334 | 9.22e-18 | 82.0 |
| MsG0180003727.01.T01 | AT3G26770 | 28.409 | 264 | 161 | 9 | 14 | 262 | 41 | 291 | 1.16e-17 | 81.3 |
| MsG0180003727.01.T01 | AT2G29300 | 28.571 | 252 | 170 | 7 | 14 | 262 | 7 | 251 | 1.49e-17 | 80.5 |
| MsG0180003727.01.T01 | AT2G29300 | 28.571 | 252 | 170 | 7 | 14 | 262 | 7 | 251 | 1.95e-17 | 80.5 |
| MsG0180003727.01.T01 | AT2G29340 | 28.400 | 250 | 172 | 5 | 14 | 262 | 7 | 250 | 2.76e-17 | 79.7 |
| MsG0180003727.01.T01 | AT2G29340 | 28.400 | 250 | 172 | 5 | 14 | 262 | 7 | 250 | 3.10e-17 | 80.1 |
| MsG0180003727.01.T01 | AT2G29320 | 28.063 | 253 | 170 | 7 | 14 | 262 | 13 | 257 | 5.29e-17 | 79.0 |
| MsG0180003727.01.T01 | AT2G29310 | 30.890 | 191 | 125 | 4 | 76 | 263 | 63 | 249 | 1.63e-16 | 77.4 |
| MsG0180003727.01.T01 | AT2G47140 | 27.344 | 256 | 171 | 8 | 14 | 263 | 6 | 252 | 2.20e-16 | 77.0 |
| MsG0180003727.01.T01 | AT3G42960 | 27.240 | 279 | 168 | 9 | 1 | 263 | 1 | 260 | 9.15e-15 | 72.8 |
| MsG0180003727.01.T01 | AT3G12800 | 28.788 | 264 | 169 | 9 | 10 | 262 | 6 | 261 | 7.39e-14 | 70.5 |
| MsG0180003727.01.T01 | AT4G05530 | 26.562 | 256 | 172 | 10 | 11 | 262 | 6 | 249 | 1.06e-13 | 69.3 |
| MsG0180003727.01.T01 | AT3G03330 | 28.155 | 206 | 143 | 4 | 14 | 217 | 41 | 243 | 1.42e-13 | 69.7 |
| MsG0180003727.01.T01 | AT2G29320 | 27.749 | 191 | 130 | 5 | 76 | 262 | 53 | 239 | 1.74e-13 | 68.9 |
| MsG0180003727.01.T01 | AT4G24050 | 27.706 | 231 | 136 | 8 | 3 | 209 | 21 | 244 | 2.62e-11 | 62.8 |
| MsG0180003727.01.T01 | AT4G24050 | 27.706 | 231 | 136 | 8 | 3 | 209 | 21 | 244 | 6.97e-11 | 62.0 |
Find 78 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCAGATTATTCGGATTAA+TGG | 0.246951 | 1:-67357380 | MsG0180003727.01.T01:CDS |
| GCCGGGGTTTGCGGCGTATA+CGG | 0.295422 | 1:-67357642 | MsG0180003727.01.T01:CDS |
| GGGTTAATGGTCAGATTATT+CGG | 0.295734 | 1:-67357388 | MsG0180003727.01.T01:CDS |
| TGGCTTCTGATGCTGGTGAA+TGG | 0.322118 | 1:-67357409 | MsG0180003727.01.T01:CDS |
| GACTAAAGATGTTGCTCCTT+TGG | 0.336164 | 1:-67357441 | MsG0180003727.01.T01:CDS |
| GCGAAGGAGTTGAAGGGAAC+TGG | 0.345340 | 1:-67357581 | MsG0180003727.01.T01:CDS |
| CAGGTTGCGGTGTTGAGGCC+GGG | 0.346418 | 1:-67357659 | MsG0180003727.01.T01:CDS |
| AGGCTCAAGCGCGGCGGTGG+TGG | 0.357017 | 1:-67357707 | MsG0180003727.01.T01:CDS |
| GATGCTGGTGAATGGGTTAA+TGG | 0.378059 | 1:-67357401 | MsG0180003727.01.T01:CDS |
| GGCTTCTGATGCTGGTGAAT+GGG | 0.388666 | 1:-67357408 | MsG0180003727.01.T01:CDS |
| ACCGATTCCGCGGGATGAAC+CGG | 0.402491 | 1:+67358075 | None:intergenic |
| AAGATGTTGCTCCTTTGGTT+GGG | 0.411244 | 1:-67357436 | MsG0180003727.01.T01:CDS |
| GCGCGCGTGAGGCGGCGAAT+AGG | 0.416184 | 1:-67357727 | MsG0180003727.01.T01:CDS |
| AAAGATGTTGCTCCTTTGGT+TGG | 0.421514 | 1:-67357437 | MsG0180003727.01.T01:CDS |
| ACCGGTTCATCCCGCGGAAT+CGG | 0.425010 | 1:-67358076 | MsG0180003727.01.T01:CDS |
| ATTATTGATGAGTCGCCGTT+AGG | 0.428931 | 1:-67357476 | MsG0180003727.01.T01:CDS |
| GCAGGTTGCGGTGTTGAGGC+CGG | 0.433126 | 1:-67357660 | MsG0180003727.01.T01:CDS |
| GTTGAAGGGAACTGGGATTA+CGG | 0.445775 | 1:-67357573 | MsG0180003727.01.T01:CDS |
| AATAGGCTCAAGCGCGGCGG+TGG | 0.446702 | 1:-67357710 | MsG0180003727.01.T01:CDS |
| TTCTATTGAAGTGTTGGCTA+TGG | 0.447331 | 1:+67357796 | None:intergenic |
| TGGCGGGAGAACGGAGGAGC+AGG | 0.461708 | 1:-67357507 | MsG0180003727.01.T01:CDS |
| AAAGCTTTCTATTGAAGTGT+TGG | 0.467005 | 1:+67357790 | None:intergenic |
| GATGTCGGCGGCGAGTGAAT+CGG | 0.467201 | 1:+67357976 | None:intergenic |
| GGCTCAAGCGCGGCGGTGGT+GGG | 0.470672 | 1:-67357706 | MsG0180003727.01.T01:CDS |
| GGTGTTGAGGCCGGGGTTTG+CGG | 0.474987 | 1:-67357651 | MsG0180003727.01.T01:CDS |
| ATCTCCGTTGCAATCGGTCC+CGG | 0.483863 | 1:+67357536 | None:intergenic |
| GCCGTATACGCCGCAAACCC+CGG | 0.488021 | 1:+67357641 | None:intergenic |
| AGGTTGCGGTGTTGAGGCCG+GGG | 0.490059 | 1:-67357658 | MsG0180003727.01.T01:CDS |
| CGCTTTCGCAACCGATTCCG+CGG | 0.492359 | 1:+67358065 | None:intergenic |
| GGATTGAATTTGAGATTGAT+CGG | 0.495307 | 1:+67357904 | None:intergenic |
| TTATTGATGAGTCGCCGTTA+GGG | 0.503638 | 1:-67357475 | MsG0180003727.01.T01:CDS |
| TACGGCGAATTGTGTTGCGC+CGG | 0.504342 | 1:-67357555 | MsG0180003727.01.T01:CDS |
| ACGGCGAATTGTGTTGCGCC+GGG | 0.507438 | 1:-67357554 | MsG0180003727.01.T01:CDS |
| TTGAGATTGATCGGAGATGT+CGG | 0.507981 | 1:+67357913 | None:intergenic |
| GACCGAGTCGCAATCATCAC+CGG | 0.518678 | 1:-67358094 | MsG0180003727.01.T01:CDS |
| ACTCGGTCTTGAAGAGGAAG+TGG | 0.525196 | 1:+67358109 | None:intergenic |
| CGAAGGAGTTGAAGGGAACT+GGG | 0.525294 | 1:-67357580 | MsG0180003727.01.T01:CDS |
| CGCGGATGACGGTGGCTCGA+GGG | 0.527973 | 1:+67357936 | None:intergenic |
| GGCGTATACGGCAGCGAAGG+CGG | 0.532044 | 1:-67357630 | MsG0180003727.01.T01:CDS |
| GCGCGGATGACGGTGGCTCG+AGG | 0.532596 | 1:+67357935 | None:intergenic |
| GAGAGAGGTGGCGTTGATGT+CGG | 0.533715 | 1:+67357961 | None:intergenic |
| TGAATCGGCGGAGAGTGAGT+TGG | 0.533901 | 1:+67357991 | None:intergenic |
| GGAGATGTCGGCGCGGATGA+CGG | 0.537171 | 1:+67357925 | None:intergenic |
| ATTGAAGTGTTGGCTATGGA+TGG | 0.543842 | 1:+67357800 | None:intergenic |
| CACATCCTCATCAATTGCGC+CGG | 0.544335 | 1:-67357842 | MsG0180003727.01.T01:CDS |
| TCAAGCGCGGCGGTGGTGGG+AGG | 0.545885 | 1:-67357703 | MsG0180003727.01.T01:CDS |
| CGGTGAGTTGAAGAAGCGTT+CGG | 0.547529 | 1:+67357868 | None:intergenic |
| CGGCGCAATTGATGAGGATG+TGG | 0.551475 | 1:+67357843 | None:intergenic |
| CAGATTATTCGGATTAATGG+TGG | 0.556383 | 1:-67357377 | MsG0180003727.01.T01:CDS |
| GAGTCGCCGTTAGGGAGGCT+TGG | 0.568046 | 1:-67357467 | MsG0180003727.01.T01:CDS |
| GCTTTCGCAACCGATTCCGC+GGG | 0.569711 | 1:+67358066 | None:intergenic |
| AGTGTTGGCTATGGATGGAT+AGG | 0.572077 | 1:+67357805 | None:intergenic |
| ATCATCACCGGTTCATCCCG+CGG | 0.576245 | 1:-67358082 | MsG0180003727.01.T01:CDS |
| TGCGGCGTATACGGCAGCGA+AGG | 0.580482 | 1:-67357633 | MsG0180003727.01.T01:CDS |
| CGTCGCAGGTTGCGGTGTTG+AGG | 0.583237 | 1:-67357664 | MsG0180003727.01.T01:CDS |
| TTCGAGCGCCGAGTGAACTC+AGG | 0.583881 | 1:+67358035 | None:intergenic |
| GGTGGCTCGAGGGAGGAGAG+AGG | 0.591553 | 1:+67357946 | None:intergenic |
| AGCGTTCGGCAGAGTCGAAG+AGG | 0.596328 | 1:+67357882 | None:intergenic |
| GGCTCGAGGGAGGAGAGAGG+TGG | 0.597935 | 1:+67357949 | None:intergenic |
| GTCGGCGGCGAGTGAATCGG+CGG | 0.601505 | 1:+67357979 | None:intergenic |
| GTCTCACCAAGCCTCCCTAA+CGG | 0.607217 | 1:+67357461 | None:intergenic |
| ATTGCGACTCGGTCTTGAAG+AGG | 0.608143 | 1:+67358103 | None:intergenic |
| TTGATGAGTCGCCGTTAGGG+AGG | 0.608872 | 1:-67357472 | MsG0180003727.01.T01:CDS |
| TGACTCCGGCGCAATTGATG+AGG | 0.609191 | 1:+67357837 | None:intergenic |
| GCGTTCGGCAGAGTCGAAGA+GGG | 0.609214 | 1:+67357883 | None:intergenic |
| GGATGACGGTGGCTCGAGGG+AGG | 0.611111 | 1:+67357939 | None:intergenic |
| GCGAATAGGCTCAAGCGCGG+CGG | 0.613768 | 1:-67357713 | MsG0180003727.01.T01:CDS |
| AGAGGTGGCGTTGATGTCGG+CGG | 0.625649 | 1:+67357964 | None:intergenic |
| GCGGCGAATAGGCTCAAGCG+CGG | 0.626379 | 1:-67357716 | MsG0180003727.01.T01:CDS |
| GCTCTTCACCTGAGTTCACT+CGG | 0.631158 | 1:-67358043 | MsG0180003727.01.T01:CDS |
| CGAACGCTTCTTCAACTCAC+CGG | 0.638935 | 1:-67357867 | MsG0180003727.01.T01:CDS |
| ATAGGTGTCATCAATGACTC+CGG | 0.642907 | 1:+67357823 | None:intergenic |
| AGTGGCTCAGTAACTCGTTG+TGG | 0.642991 | 1:+67358127 | None:intergenic |
| AACCGGTGATGATTGCGACT+CGG | 0.644911 | 1:+67358092 | None:intergenic |
| GATGTCGGCGCGGATGACGG+TGG | 0.646731 | 1:+67357928 | None:intergenic |
| TGCGCCGGGACCGATTGCAA+CGG | 0.653880 | 1:-67357540 | MsG0180003727.01.T01:CDS |
| CAATTGATGAGGATGTGGAC+CGG | 0.668438 | 1:+67357848 | None:intergenic |
| ATTGATCGGAGATGTCGGCG+CGG | 0.754315 | 1:+67357918 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCTTTCTATTGAAGTGT+TGG | + | Chr1:67357719-67357738 | None:intergenic | 30.0% | |
| ! | GGATTGAATTTGAGATTGAT+CGG | + | Chr1:67357605-67357624 | None:intergenic | 30.0% |
| AAAAACATCTCCGTTGCAAT+CGG | + | Chr1:67357979-67357998 | None:intergenic | 35.0% | |
| CAGATTATTCGGATTAATGG+TGG | - | Chr1:67358129-67358148 | MsG0180003727.01.T01:CDS | 35.0% | |
| GGGTTAATGGTCAGATTATT+CGG | - | Chr1:67358118-67358137 | MsG0180003727.01.T01:CDS | 35.0% | |
| GGTCAGATTATTCGGATTAA+TGG | - | Chr1:67358126-67358145 | MsG0180003727.01.T01:CDS | 35.0% | |
| !! | ATTTTAGCGAAGGAGTTGAA+GGG | - | Chr1:67357919-67357938 | MsG0180003727.01.T01:CDS | 35.0% |
| !! | TATGACGAAGATTTTAGCGA+AGG | - | Chr1:67357909-67357928 | MsG0180003727.01.T01:CDS | 35.0% |
| !! | TTCTATTGAAGTGTTGGCTA+TGG | + | Chr1:67357713-67357732 | None:intergenic | 35.0% |
| !!! | ATTGCAACGGAGATGTTTTT+TGG | - | Chr1:67357979-67357998 | MsG0180003727.01.T01:CDS | 35.0% |
| AAAGATGTTGCTCCTTTGGT+TGG | - | Chr1:67358069-67358088 | MsG0180003727.01.T01:CDS | 40.0% | |
| AAGATGTTGCTCCTTTGGTT+GGG | - | Chr1:67358070-67358089 | MsG0180003727.01.T01:CDS | 40.0% | |
| ATTATTGATGAGTCGCCGTT+AGG | - | Chr1:67358030-67358049 | MsG0180003727.01.T01:CDS | 40.0% | |
| GACTAAAGATGTTGCTCCTT+TGG | - | Chr1:67358065-67358084 | MsG0180003727.01.T01:CDS | 40.0% | |
| TTATTGATGAGTCGCCGTTA+GGG | - | Chr1:67358031-67358050 | MsG0180003727.01.T01:CDS | 40.0% | |
| TTGAGATTGATCGGAGATGT+CGG | + | Chr1:67357596-67357615 | None:intergenic | 40.0% | |
| ! | AATACTATTTTCGTCGTCGC+AGG | - | Chr1:67357828-67357847 | MsG0180003727.01.T01:CDS | 40.0% |
| ! | ATAGGTGTCATCAATGACTC+CGG | + | Chr1:67357686-67357705 | None:intergenic | 40.0% |
| !! | ATTGAAGTGTTGGCTATGGA+TGG | + | Chr1:67357709-67357728 | None:intergenic | 40.0% |
| !! | GATTTTAGCGAAGGAGTTGA+AGG | - | Chr1:67357918-67357937 | MsG0180003727.01.T01:CDS | 40.0% |
| CAATTGATGAGGATGTGGAC+CGG | + | Chr1:67357661-67357680 | None:intergenic | 45.0% | |
| GAAGCCAAAAACCCAACCAA+AGG | + | Chr1:67358084-67358103 | None:intergenic | 45.0% | |
| GTTGAAGGGAACTGGGATTA+CGG | - | Chr1:67357933-67357952 | MsG0180003727.01.T01:CDS | 45.0% | |
| ! | TGCTCCTTTGGTTGGGTTTT+TGG | - | Chr1:67358077-67358096 | MsG0180003727.01.T01:CDS | 45.0% |
| !! | AGTGTTGGCTATGGATGGAT+AGG | + | Chr1:67357704-67357723 | None:intergenic | 45.0% |
| !! | GAAAGCTTTGACCGCGTTTT+TGG | - | Chr1:67357730-67357749 | MsG0180003727.01.T01:CDS | 45.0% |
| !! | GATGCTGGTGAATGGGTTAA+TGG | - | Chr1:67358105-67358124 | MsG0180003727.01.T01:CDS | 45.0% |
| !!! | CAACGGAGATGTTTTTTGGC+GGG | - | Chr1:67357983-67358002 | MsG0180003727.01.T01:CDS | 45.0% |
| !!! | GATGTTTTTTGGCGGGAGAA+CGG | - | Chr1:67357990-67358009 | MsG0180003727.01.T01:CDS | 45.0% |
| !!! | GCAACGGAGATGTTTTTTGG+CGG | - | Chr1:67357982-67358001 | MsG0180003727.01.T01:CDS | 45.0% |
| AACCGGTGATGATTGCGACT+CGG | + | Chr1:67357417-67357436 | None:intergenic | 50.0% | |
| ACTCGGTCTTGAAGAGGAAG+TGG | + | Chr1:67357400-67357419 | None:intergenic | 50.0% | |
| AGTGGCTCAGTAACTCGTTG+TGG | + | Chr1:67357382-67357401 | None:intergenic | 50.0% | |
| CACATCCTCATCAATTGCGC+CGG | - | Chr1:67357664-67357683 | MsG0180003727.01.T01:CDS | 50.0% | |
| CGAACGCTTCTTCAACTCAC+CGG | - | Chr1:67357639-67357658 | MsG0180003727.01.T01:CDS | 50.0% | |
| CGAAGGAGTTGAAGGGAACT+GGG | - | Chr1:67357926-67357945 | MsG0180003727.01.T01:CDS | 50.0% | |
| GCTCTTCACCTGAGTTCACT+CGG | - | Chr1:67357463-67357482 | MsG0180003727.01.T01:CDS | 50.0% | |
| GGCTTCTGATGCTGGTGAAT+GGG | - | Chr1:67358098-67358117 | MsG0180003727.01.T01:CDS | 50.0% | |
| TGGCTTCTGATGCTGGTGAA+TGG | - | Chr1:67358097-67358116 | MsG0180003727.01.T01:CDS | 50.0% | |
| ! | ATTTTCGTCGTCGCAGGTTG+CGG | - | Chr1:67357834-67357853 | MsG0180003727.01.T01:CDS | 50.0% |
| !! | ATTGCGACTCGGTCTTGAAG+AGG | + | Chr1:67357406-67357425 | None:intergenic | 50.0% |
| !! | CGGTGAGTTGAAGAAGCGTT+CGG | + | Chr1:67357641-67357660 | None:intergenic | 50.0% |
| !!! | GGGTTTTTGGCTTCTGATGC+TGG | - | Chr1:67358090-67358109 | MsG0180003727.01.T01:CDS | 50.0% |
| ATCTCCGTTGCAATCGGTCC+CGG | + | Chr1:67357973-67357992 | None:intergenic | 55.0% | |
| ATTGATCGGAGATGTCGGCG+CGG | + | Chr1:67357591-67357610 | None:intergenic | 55.0% | |
| CGGCGCAATTGATGAGGATG+TGG | + | Chr1:67357666-67357685 | None:intergenic | 55.0% | |
| GACCGAGTCGCAATCATCAC+CGG | - | Chr1:67357412-67357431 | MsG0180003727.01.T01:CDS | 55.0% | |
| GCGAAGGAGTTGAAGGGAAC+TGG | - | Chr1:67357925-67357944 | MsG0180003727.01.T01:CDS | 55.0% | |
| GCGCGTTCACTCCAAAAACG+CGG | + | Chr1:67357744-67357763 | None:intergenic | 55.0% | |
| GTCTCACCAAGCCTCCCTAA+CGG | + | Chr1:67358048-67358067 | None:intergenic | 55.0% | |
| TACGGCGAATTGTGTTGCGC+CGG | - | Chr1:67357951-67357970 | MsG0180003727.01.T01:CDS | 55.0% | |
| TGAATCGGCGGAGAGTGAGT+TGG | + | Chr1:67357518-67357537 | None:intergenic | 55.0% | |
| TGACTCCGGCGCAATTGATG+AGG | + | Chr1:67357672-67357691 | None:intergenic | 55.0% | |
| TTGATGAGTCGCCGTTAGGG+AGG | - | Chr1:67358034-67358053 | MsG0180003727.01.T01:CDS | 55.0% | |
| ! | GTTTTTATGCGCGCGTGAGG+CGG | - | Chr1:67357771-67357790 | MsG0180003727.01.T01:CDS | 55.0% |
| ! | TGCGTTTTTATGCGCGCGTG+AGG | - | Chr1:67357768-67357787 | MsG0180003727.01.T01:CDS | 55.0% |
| !! | ATCATCACCGGTTCATCCCG+CGG | - | Chr1:67357424-67357443 | MsG0180003727.01.T01:CDS | 55.0% |
| !! | GAGAGAGGTGGCGTTGATGT+CGG | + | Chr1:67357548-67357567 | None:intergenic | 55.0% |
| !!! | GTTTTTGGAGTGAACGCGCG+TGG | - | Chr1:67357745-67357764 | MsG0180003727.01.T01:CDS | 55.0% |
| !!! | GTTTTTTGGCGGGAGAACGG+AGG | - | Chr1:67357993-67358012 | MsG0180003727.01.T01:CDS | 55.0% |
| ACGGCGAATTGTGTTGCGCC+GGG | - | Chr1:67357952-67357971 | MsG0180003727.01.T01:CDS | 60.0% | |
| AGCGTTCGGCAGAGTCGAAG+AGG | + | Chr1:67357627-67357646 | None:intergenic | 60.0% | |
| GATGTCGGCGGCGAGTGAAT+CGG | + | Chr1:67357533-67357552 | None:intergenic | 60.0% | |
| GCGTTCGGCAGAGTCGAAGA+GGG | + | Chr1:67357626-67357645 | None:intergenic | 60.0% | |
| ! | ACCGATTCCGCGGGATGAAC+CGG | + | Chr1:67357434-67357453 | None:intergenic | 60.0% |
| ! | ACCGGTTCATCCCGCGGAAT+CGG | - | Chr1:67357430-67357449 | MsG0180003727.01.T01:CDS | 60.0% |
| ! | CGCTTTCGCAACCGATTCCG+CGG | + | Chr1:67357444-67357463 | None:intergenic | 60.0% |
| ! | GCTTTCGCAACCGATTCCGC+GGG | + | Chr1:67357443-67357462 | None:intergenic | 60.0% |
| !! | AGAGGTGGCGTTGATGTCGG+CGG | + | Chr1:67357545-67357564 | None:intergenic | 60.0% |
| !! | TTCGAGCGCCGAGTGAACTC+AGG | + | Chr1:67357474-67357493 | None:intergenic | 60.0% |
| AATAGGCTCAAGCGCGGCGG+TGG | - | Chr1:67357796-67357815 | MsG0180003727.01.T01:CDS | 65.0% | |
| GAGTCGCCGTTAGGGAGGCT+TGG | - | Chr1:67358039-67358058 | MsG0180003727.01.T01:CDS | 65.0% | |
| GCCGGGGTTTGCGGCGTATA+CGG | - | Chr1:67357864-67357883 | MsG0180003727.01.T01:CDS | 65.0% | |
| GCCGTATACGCCGCAAACCC+CGG | + | Chr1:67357868-67357887 | None:intergenic | 65.0% | |
| GCGAATAGGCTCAAGCGCGG+CGG | - | Chr1:67357793-67357812 | MsG0180003727.01.T01:CDS | 65.0% | |
| GCGGCGAATAGGCTCAAGCG+CGG | - | Chr1:67357790-67357809 | MsG0180003727.01.T01:CDS | 65.0% | |
| GGAGATGTCGGCGCGGATGA+CGG | + | Chr1:67357584-67357603 | None:intergenic | 65.0% | |
| GGCGTATACGGCAGCGAAGG+CGG | - | Chr1:67357876-67357895 | MsG0180003727.01.T01:CDS | 65.0% | |
| TGCGCCGGGACCGATTGCAA+CGG | - | Chr1:67357966-67357985 | MsG0180003727.01.T01:CDS | 65.0% | |
| TGCGGCGTATACGGCAGCGA+AGG | - | Chr1:67357873-67357892 | MsG0180003727.01.T01:CDS | 65.0% | |
| ! | AGGTTGCGGTGTTGAGGCCG+GGG | - | Chr1:67357848-67357867 | MsG0180003727.01.T01:CDS | 65.0% |
| ! | CAGGTTGCGGTGTTGAGGCC+GGG | - | Chr1:67357847-67357866 | MsG0180003727.01.T01:CDS | 65.0% |
| ! | CGTCGCAGGTTGCGGTGTTG+AGG | - | Chr1:67357842-67357861 | MsG0180003727.01.T01:CDS | 65.0% |
| ! | GCAGGTTGCGGTGTTGAGGC+CGG | - | Chr1:67357846-67357865 | MsG0180003727.01.T01:CDS | 65.0% |
| ! | GGTGTTGAGGCCGGGGTTTG+CGG | - | Chr1:67357855-67357874 | MsG0180003727.01.T01:CDS | 65.0% |
| GGCTCGAGGGAGGAGAGAGG+TGG | + | Chr1:67357560-67357579 | None:intergenic | 70.0% | |
| GGTGGCTCGAGGGAGGAGAG+AGG | + | Chr1:67357563-67357582 | None:intergenic | 70.0% | |
| GTCGGCGGCGAGTGAATCGG+CGG | + | Chr1:67357530-67357549 | None:intergenic | 70.0% | |
| TGGCGGGAGAACGGAGGAGC+AGG | - | Chr1:67357999-67358018 | MsG0180003727.01.T01:CDS | 70.0% | |
| ! | CGCGGATGACGGTGGCTCGA+GGG | + | Chr1:67357573-67357592 | None:intergenic | 70.0% |
| ! | GATGTCGGCGCGGATGACGG+TGG | + | Chr1:67357581-67357600 | None:intergenic | 70.0% |
| ! | GGATGACGGTGGCTCGAGGG+AGG | + | Chr1:67357570-67357589 | None:intergenic | 70.0% |
| AGGCTCAAGCGCGGCGGTGG+TGG | - | Chr1:67357799-67357818 | MsG0180003727.01.T01:CDS | 75.0% | |
| GCGCGCGTGAGGCGGCGAAT+AGG | - | Chr1:67357779-67357798 | MsG0180003727.01.T01:CDS | 75.0% | |
| GGCTCAAGCGCGGCGGTGGT+GGG | - | Chr1:67357800-67357819 | MsG0180003727.01.T01:CDS | 75.0% | |
| ! | GCGCGGATGACGGTGGCTCG+AGG | + | Chr1:67357574-67357593 | None:intergenic | 75.0% |
| !! | TCAAGCGCGGCGGTGGTGGG+AGG | - | Chr1:67357803-67357822 | MsG0180003727.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 67357367 | 67358161 | 67357367 | ID=MsG0180003727.01;Name=MsG0180003727.01 |
| Chr1 | mRNA | 67357367 | 67358161 | 67357367 | ID=MsG0180003727.01.T01;Parent=MsG0180003727.01;Name=MsG0180003727.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|264 |
| Chr1 | exon | 67357367 | 67358161 | 67357367 | ID=MsG0180003727.01.T01:exon:24075;Parent=MsG0180003727.01.T01 |
| Chr1 | CDS | 67357367 | 67358161 | 67357367 | ID=MsG0180003727.01.T01:cds;Parent=MsG0180003727.01.T01 |
| Gene Sequence |
| Protein sequence |
