Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032061.01.T01 | KEH25863.1 | 87.645 | 259 | 19 | 1 | 7 | 265 | 3 | 248 | 1.40E-155 | 443 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032061.01.T01 | Q94K41 | 62.698 | 252 | 94 | 0 | 7 | 258 | 4 | 255 | 9.27E-113 | 327 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032061.01.T01 | A0A072U9Z2 | 87.645 | 259 | 19 | 1 | 7 | 265 | 3 | 248 | 6.67e-156 | 443 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032061.01.T01 | MTR_6g035395 | 87.645 | 259 | 19 | 1 | 7 | 265 | 3 | 248 | 1.69e-159 | 443 |
| MsG0680032061.01.T01 | MTR_7g110140 | 78.175 | 252 | 55 | 0 | 9 | 260 | 6 | 257 | 1.67e-142 | 400 |
| MsG0680032061.01.T01 | MTR_7g110130 | 70.196 | 255 | 75 | 1 | 7 | 260 | 4 | 258 | 8.40e-125 | 356 |
| MsG0680032061.01.T01 | MTR_2g090165 | 67.194 | 253 | 83 | 0 | 7 | 259 | 4 | 256 | 9.54e-122 | 348 |
| MsG0680032061.01.T01 | MTR_7g110160 | 62.055 | 253 | 78 | 1 | 7 | 259 | 4 | 238 | 1.73e-104 | 303 |
| MsG0680032061.01.T01 | MTR_6g023590 | 52.000 | 250 | 118 | 2 | 9 | 256 | 13 | 262 | 3.14e-85 | 255 |
| MsG0680032061.01.T01 | MTR_6g023950 | 51.587 | 252 | 120 | 2 | 9 | 258 | 13 | 264 | 5.33e-81 | 244 |
| MsG0680032061.01.T01 | MTR_6g023610 | 51.807 | 249 | 118 | 2 | 9 | 255 | 13 | 261 | 1.33e-80 | 244 |
| MsG0680032061.01.T01 | MTR_4g052350 | 52.510 | 259 | 115 | 4 | 3 | 260 | 11 | 262 | 7.64e-80 | 242 |
| MsG0680032061.01.T01 | MTR_6g023910 | 51.807 | 249 | 118 | 2 | 9 | 255 | 13 | 261 | 9.71e-80 | 241 |
| MsG0680032061.01.T01 | MTR_4g052340 | 50.965 | 259 | 119 | 4 | 3 | 260 | 11 | 262 | 3.91e-79 | 240 |
| MsG0680032061.01.T01 | MTR_6g023600 | 50.800 | 250 | 121 | 2 | 9 | 256 | 13 | 262 | 2.53e-78 | 238 |
| MsG0680032061.01.T01 | MTR_6g023990 | 49.600 | 250 | 124 | 2 | 9 | 256 | 14 | 263 | 3.70e-78 | 237 |
| MsG0680032061.01.T01 | MTR_4g052320 | 50.988 | 253 | 120 | 3 | 9 | 260 | 8 | 257 | 4.15e-76 | 232 |
| MsG0680032061.01.T01 | MTR_7g071680 | 48.175 | 274 | 120 | 4 | 7 | 260 | 27 | 298 | 1.90e-75 | 232 |
| MsG0680032061.01.T01 | MTR_4g052300 | 49.807 | 259 | 125 | 3 | 3 | 260 | 2 | 256 | 9.96e-75 | 229 |
| MsG0680032061.01.T01 | MTR_6g023600 | 50.000 | 250 | 117 | 3 | 9 | 256 | 13 | 256 | 2.11e-73 | 225 |
| MsG0680032061.01.T01 | MTR_7g105460 | 47.619 | 252 | 130 | 2 | 9 | 259 | 13 | 263 | 1.08e-72 | 224 |
| MsG0680032061.01.T01 | MTR_7g083870 | 47.893 | 261 | 127 | 5 | 7 | 260 | 33 | 291 | 2.45e-68 | 213 |
| MsG0680032061.01.T01 | MTR_7g107670 | 45.627 | 263 | 133 | 6 | 7 | 262 | 31 | 290 | 3.28e-68 | 213 |
| MsG0680032061.01.T01 | MTR_7g105580 | 45.276 | 254 | 121 | 3 | 6 | 259 | 5 | 240 | 6.61e-63 | 198 |
| MsG0680032061.01.T01 | MTR_4g078630 | 43.071 | 267 | 141 | 5 | 7 | 265 | 17 | 280 | 1.05e-61 | 196 |
| MsG0680032061.01.T01 | MTR_3g020670 | 46.241 | 266 | 133 | 5 | 6 | 262 | 13 | 277 | 3.60e-58 | 187 |
| MsG0680032061.01.T01 | MTR_7g105590 | 48.108 | 185 | 94 | 2 | 7 | 190 | 6 | 189 | 4.43e-52 | 168 |
| MsG0680032061.01.T01 | MTR_3g113870 | 41.206 | 199 | 113 | 4 | 1 | 197 | 12 | 208 | 1.14e-44 | 150 |
| MsG0680032061.01.T01 | MTR_7g105570 | 37.354 | 257 | 108 | 3 | 4 | 259 | 16 | 220 | 9.22e-44 | 148 |
| MsG0680032061.01.T01 | MTR_4g084800 | 50.000 | 134 | 66 | 1 | 2 | 134 | 6 | 139 | 1.67e-38 | 132 |
| MsG0680032061.01.T01 | MTR_4g058840 | 28.261 | 276 | 167 | 8 | 1 | 257 | 1 | 264 | 2.32e-23 | 96.3 |
| MsG0680032061.01.T01 | MTR_4g058820 | 28.261 | 276 | 167 | 8 | 1 | 257 | 1 | 264 | 2.32e-23 | 96.3 |
| MsG0680032061.01.T01 | MTR_3g085740 | 29.804 | 255 | 160 | 9 | 9 | 255 | 75 | 318 | 1.29e-20 | 89.7 |
| MsG0680032061.01.T01 | MTR_1g051165 | 26.054 | 261 | 179 | 6 | 9 | 257 | 14 | 272 | 2.08e-20 | 88.6 |
| MsG0680032061.01.T01 | MTR_4g058810 | 27.170 | 265 | 166 | 8 | 9 | 257 | 11 | 264 | 3.37e-18 | 82.0 |
| MsG0680032061.01.T01 | MTR_8g442750 | 30.153 | 262 | 158 | 10 | 9 | 255 | 14 | 265 | 3.58e-17 | 79.7 |
| MsG0680032061.01.T01 | MTR_1196s0010 | 32.283 | 254 | 149 | 9 | 10 | 255 | 275 | 513 | 6.46e-17 | 80.5 |
| MsG0680032061.01.T01 | MTR_7g113650 | 27.969 | 261 | 171 | 6 | 4 | 254 | 35 | 288 | 1.16e-16 | 78.2 |
| MsG0680032061.01.T01 | MTR_4g132850 | 29.365 | 252 | 167 | 7 | 7 | 255 | 5 | 248 | 1.71e-16 | 77.0 |
| MsG0680032061.01.T01 | MTR_4g064887 | 26.437 | 261 | 177 | 8 | 4 | 257 | 9 | 261 | 2.50e-16 | 77.0 |
| MsG0680032061.01.T01 | MTR_4g097510 | 30.220 | 182 | 123 | 4 | 10 | 190 | 3 | 181 | 1.39e-15 | 75.1 |
| MsG0680032061.01.T01 | MTR_7g105560 | 57.377 | 61 | 26 | 0 | 2 | 62 | 6 | 66 | 4.41e-15 | 71.2 |
| MsG0680032061.01.T01 | MTR_1g082760 | 26.415 | 265 | 160 | 7 | 9 | 254 | 40 | 288 | 9.47e-15 | 72.8 |
| MsG0680032061.01.T01 | MTR_4g131740 | 26.054 | 261 | 167 | 8 | 9 | 254 | 40 | 289 | 1.54e-14 | 72.4 |
| MsG0680032061.01.T01 | MTR_7g094520 | 28.846 | 260 | 173 | 7 | 3 | 255 | 5 | 259 | 4.41e-14 | 70.5 |
| MsG0680032061.01.T01 | MTR_3g068085 | 29.644 | 253 | 168 | 10 | 9 | 257 | 15 | 261 | 5.96e-14 | 70.5 |
| MsG0680032061.01.T01 | MTR_3g087850 | 30.556 | 180 | 117 | 5 | 13 | 190 | 30 | 203 | 6.50e-14 | 70.5 |
| MsG0680032061.01.T01 | MTR_8g096580 | 30.928 | 194 | 127 | 4 | 9 | 198 | 45 | 235 | 1.48e-13 | 69.7 |
| MsG0680032061.01.T01 | MTR_4g086400 | 25.287 | 261 | 154 | 8 | 11 | 251 | 6 | 245 | 4.09e-13 | 69.3 |
| MsG0680032061.01.T01 | MTR_7g063360 | 28.788 | 264 | 173 | 10 | 3 | 257 | 9 | 266 | 4.19e-13 | 67.8 |
| MsG0680032061.01.T01 | MTR_7g063390 | 28.409 | 264 | 174 | 9 | 3 | 257 | 9 | 266 | 7.52e-13 | 67.0 |
| MsG0680032061.01.T01 | MTR_3g087860 | 33.835 | 133 | 84 | 3 | 59 | 190 | 59 | 188 | 5.20e-12 | 65.1 |
| MsG0680032061.01.T01 | MTR_7g063320 | 27.969 | 261 | 179 | 8 | 1 | 255 | 6 | 263 | 5.60e-12 | 64.7 |
| MsG0680032061.01.T01 | MTR_7g094510 | 26.275 | 255 | 174 | 7 | 9 | 255 | 6 | 254 | 1.91e-11 | 63.2 |
| MsG0680032061.01.T01 | MTR_4g064887 | 27.723 | 202 | 134 | 6 | 4 | 198 | 9 | 205 | 3.22e-11 | 62.0 |
| MsG0680032061.01.T01 | MTR_4g066050 | 27.168 | 173 | 120 | 3 | 12 | 178 | 27 | 199 | 5.46e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032061.01.T01 | AT2G47140 | 62.698 | 252 | 94 | 0 | 7 | 258 | 4 | 255 | 9.45e-114 | 327 |
| MsG0680032061.01.T01 | AT3G29250 | 62.000 | 250 | 95 | 0 | 9 | 258 | 6 | 255 | 1.59e-106 | 309 |
| MsG0680032061.01.T01 | AT3G29250 | 62.000 | 250 | 95 | 0 | 9 | 258 | 44 | 293 | 5.01e-106 | 309 |
| MsG0680032061.01.T01 | AT3G29250 | 62.000 | 250 | 95 | 0 | 9 | 258 | 42 | 291 | 7.10e-106 | 309 |
| MsG0680032061.01.T01 | AT3G29260 | 59.127 | 252 | 102 | 1 | 7 | 258 | 4 | 254 | 1.20e-100 | 294 |
| MsG0680032061.01.T01 | AT2G47130 | 58.103 | 253 | 105 | 1 | 9 | 261 | 6 | 257 | 2.39e-100 | 293 |
| MsG0680032061.01.T01 | AT2G47120 | 56.800 | 250 | 106 | 2 | 9 | 258 | 6 | 253 | 6.56e-97 | 285 |
| MsG0680032061.01.T01 | AT2G47120 | 56.800 | 250 | 106 | 2 | 9 | 258 | 7 | 254 | 7.74e-97 | 285 |
| MsG0680032061.01.T01 | AT3G51680 | 48.889 | 270 | 122 | 3 | 7 | 260 | 30 | 299 | 1.97e-74 | 229 |
| MsG0680032061.01.T01 | AT4G03140 | 43.678 | 261 | 139 | 5 | 7 | 261 | 76 | 334 | 5.86e-63 | 201 |
| MsG0680032061.01.T01 | AT3G26760 | 41.887 | 265 | 143 | 4 | 6 | 261 | 33 | 295 | 6.79e-63 | 199 |
| MsG0680032061.01.T01 | AT1G52340 | 45.149 | 268 | 134 | 6 | 7 | 262 | 16 | 282 | 2.04e-62 | 198 |
| MsG0680032061.01.T01 | AT3G26770 | 41.860 | 258 | 141 | 4 | 6 | 256 | 38 | 293 | 7.23e-61 | 194 |
| MsG0680032061.01.T01 | AT3G42960 | 43.629 | 259 | 134 | 4 | 7 | 256 | 6 | 261 | 2.70e-53 | 174 |
| MsG0680032061.01.T01 | AT2G47150 | 38.340 | 253 | 103 | 6 | 9 | 261 | 1 | 200 | 2.05e-40 | 139 |
| MsG0680032061.01.T01 | AT2G17845 | 27.341 | 267 | 168 | 8 | 9 | 258 | 47 | 304 | 3.00e-18 | 83.2 |
| MsG0680032061.01.T01 | AT4G05530 | 32.184 | 261 | 160 | 7 | 1 | 255 | 1 | 250 | 1.05e-17 | 80.5 |
| MsG0680032061.01.T01 | AT2G29150 | 31.579 | 266 | 154 | 12 | 5 | 257 | 11 | 261 | 2.18e-17 | 80.1 |
| MsG0680032061.01.T01 | AT3G55290 | 27.715 | 267 | 162 | 8 | 9 | 257 | 17 | 270 | 5.56e-17 | 79.0 |
| MsG0680032061.01.T01 | AT3G55290 | 27.715 | 267 | 162 | 8 | 9 | 257 | 18 | 271 | 5.91e-17 | 79.0 |
| MsG0680032061.01.T01 | AT1G63380 | 27.341 | 267 | 164 | 9 | 9 | 257 | 15 | 269 | 1.41e-16 | 77.8 |
| MsG0680032061.01.T01 | AT1G63380 | 27.341 | 267 | 164 | 9 | 9 | 257 | 22 | 276 | 1.58e-16 | 77.8 |
| MsG0680032061.01.T01 | AT1G63380 | 27.341 | 267 | 164 | 9 | 9 | 257 | 17 | 271 | 1.61e-16 | 77.8 |
| MsG0680032061.01.T01 | AT1G63380 | 27.341 | 267 | 164 | 9 | 9 | 257 | 19 | 273 | 1.82e-16 | 77.8 |
| MsG0680032061.01.T01 | AT2G30670 | 29.297 | 256 | 170 | 8 | 7 | 257 | 4 | 253 | 2.70e-16 | 76.6 |
| MsG0680032061.01.T01 | AT1G07450 | 29.183 | 257 | 168 | 9 | 7 | 257 | 5 | 253 | 3.21e-16 | 76.6 |
| MsG0680032061.01.T01 | AT1G62610 | 27.027 | 259 | 169 | 7 | 9 | 254 | 19 | 270 | 4.74e-16 | 76.6 |
| MsG0680032061.01.T01 | AT1G62610 | 27.027 | 259 | 169 | 7 | 9 | 254 | 13 | 264 | 4.87e-16 | 76.3 |
| MsG0680032061.01.T01 | AT1G62610 | 27.027 | 259 | 169 | 7 | 9 | 254 | 15 | 266 | 5.14e-16 | 76.3 |
| MsG0680032061.01.T01 | AT1G62610 | 27.027 | 259 | 169 | 7 | 9 | 254 | 14 | 265 | 5.18e-16 | 76.3 |
| MsG0680032061.01.T01 | AT2G29340 | 29.020 | 255 | 172 | 7 | 7 | 257 | 4 | 253 | 5.27e-16 | 75.9 |
| MsG0680032061.01.T01 | AT2G29340 | 29.020 | 255 | 172 | 7 | 7 | 257 | 4 | 253 | 6.84e-16 | 76.3 |
| MsG0680032061.01.T01 | AT3G55310 | 28.030 | 264 | 159 | 8 | 9 | 254 | 17 | 267 | 9.91e-16 | 75.5 |
| MsG0680032061.01.T01 | AT2G29370 | 30.682 | 264 | 160 | 11 | 5 | 257 | 11 | 262 | 1.31e-15 | 75.1 |
| MsG0680032061.01.T01 | AT1G07440 | 30.233 | 258 | 164 | 10 | 7 | 257 | 9 | 257 | 2.39e-15 | 74.3 |
| MsG0680032061.01.T01 | AT2G29310 | 28.405 | 257 | 171 | 8 | 7 | 257 | 4 | 253 | 2.44e-15 | 74.3 |
| MsG0680032061.01.T01 | AT2G29360 | 30.370 | 270 | 153 | 13 | 5 | 257 | 11 | 262 | 2.70e-15 | 74.3 |
| MsG0680032061.01.T01 | AT3G46170 | 25.573 | 262 | 168 | 7 | 9 | 254 | 26 | 276 | 1.07e-14 | 72.8 |
| MsG0680032061.01.T01 | AT3G12800 | 28.462 | 260 | 166 | 9 | 9 | 255 | 10 | 262 | 1.28e-14 | 72.8 |
| MsG0680032061.01.T01 | AT2G29330 | 29.183 | 257 | 169 | 8 | 7 | 257 | 4 | 253 | 1.55e-14 | 72.0 |
| MsG0680032061.01.T01 | AT5G19200 | 32.547 | 212 | 131 | 5 | 15 | 220 | 41 | 246 | 1.71e-14 | 72.4 |
| MsG0680032061.01.T01 | AT3G06060 | 30.275 | 218 | 140 | 5 | 9 | 220 | 54 | 265 | 2.06e-14 | 72.4 |
| MsG0680032061.01.T01 | AT3G06060 | 30.275 | 218 | 140 | 5 | 9 | 220 | 37 | 248 | 2.19e-14 | 72.4 |
| MsG0680032061.01.T01 | AT1G24360 | 27.626 | 257 | 167 | 10 | 9 | 257 | 74 | 319 | 2.38e-14 | 72.0 |
| MsG0680032061.01.T01 | AT2G29290 | 30.075 | 266 | 155 | 10 | 7 | 257 | 4 | 253 | 2.62e-14 | 71.2 |
| MsG0680032061.01.T01 | AT5G06060 | 28.958 | 259 | 167 | 9 | 7 | 257 | 6 | 255 | 2.90e-14 | 71.2 |
| MsG0680032061.01.T01 | AT5G18210 | 30.620 | 258 | 162 | 8 | 9 | 255 | 8 | 259 | 8.03e-14 | 69.7 |
| MsG0680032061.01.T01 | AT1G54870 | 27.692 | 260 | 163 | 7 | 9 | 254 | 82 | 330 | 1.42e-13 | 70.1 |
| MsG0680032061.01.T01 | AT1G54870 | 27.692 | 260 | 163 | 7 | 9 | 254 | 84 | 332 | 1.54e-13 | 69.7 |
| MsG0680032061.01.T01 | AT5G65205 | 29.255 | 188 | 125 | 5 | 10 | 195 | 7 | 188 | 2.92e-13 | 68.6 |
| MsG0680032061.01.T01 | AT1G49670 | 28.311 | 219 | 126 | 6 | 14 | 212 | 9 | 216 | 1.33e-12 | 67.8 |
| MsG0680032061.01.T01 | AT1G49670 | 28.311 | 219 | 126 | 6 | 14 | 212 | 9 | 216 | 1.37e-12 | 67.8 |
| MsG0680032061.01.T01 | AT1G10310 | 28.571 | 182 | 123 | 4 | 12 | 190 | 18 | 195 | 3.27e-12 | 65.1 |
| MsG0680032061.01.T01 | AT3G05260 | 26.154 | 260 | 172 | 8 | 9 | 257 | 37 | 287 | 3.75e-12 | 65.5 |
| MsG0680032061.01.T01 | AT4G13180 | 29.457 | 258 | 166 | 7 | 9 | 257 | 13 | 263 | 7.26e-12 | 64.3 |
| MsG0680032061.01.T01 | AT5G50770 | 31.148 | 183 | 120 | 3 | 11 | 190 | 47 | 226 | 8.56e-12 | 64.7 |
| MsG0680032061.01.T01 | AT2G29320 | 29.057 | 265 | 165 | 9 | 4 | 257 | 8 | 260 | 9.96e-12 | 63.9 |
| MsG0680032061.01.T01 | AT5G50600 | 30.460 | 174 | 116 | 3 | 9 | 179 | 45 | 216 | 1.16e-11 | 64.3 |
| MsG0680032061.01.T01 | AT5G50700 | 30.460 | 174 | 116 | 3 | 9 | 179 | 45 | 216 | 1.16e-11 | 64.3 |
| MsG0680032061.01.T01 | AT2G29340 | 29.082 | 196 | 133 | 5 | 7 | 198 | 4 | 197 | 2.31e-11 | 62.0 |
| MsG0680032061.01.T01 | AT2G29340 | 29.082 | 196 | 133 | 5 | 7 | 198 | 4 | 197 | 2.31e-11 | 62.0 |
Find 56 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAATAGTGTTTCCCCTTT+TGG | 0.226669 | 6:-32913005 | MsG0680032061.01.T01:CDS |
| GTAGACATTCAAGATGAATT+AGG | 0.245367 | 6:-32913437 | MsG0680032061.01.T01:CDS |
| TGCATCAAAACATGCTCTTT+TGG | 0.245464 | 6:-32913075 | MsG0680032061.01.T01:CDS |
| TTACATCAGTGGTCATAATT+TGG | 0.260914 | 6:-32912832 | MsG0680032061.01.T01:CDS |
| GTGAAGTTGTTCGTCGAAAA+TGG | 0.282900 | 6:-32913476 | MsG0680032061.01.T01:CDS |
| TTCTTGAACATTTAGGTTAG+AGG | 0.321097 | 6:-32913549 | MsG0680032061.01.T01:intron |
| TTCAGCAATGCCGGAATATT+AGG | 0.326721 | 6:-32913287 | MsG0680032061.01.T01:CDS |
| GGAAGCGATCGTAAGTTCTA+AGG | 0.349654 | 6:-32912931 | MsG0680032061.01.T01:CDS |
| TGTAGTGAACTTGGTTGTTA+TGG | 0.360765 | 6:-32913035 | MsG0680032061.01.T01:CDS |
| TGCCCCTCAGAAGGGCAAAA+TGG | 0.374110 | 6:-32914494 | MsG0680032061.01.T01:intron |
| CGGAATATTAGGCTCTTCAT+TGG | 0.381028 | 6:-32913276 | MsG0680032061.01.T01:CDS |
| CAATGAAGAGCCTAATATTC+CGG | 0.389833 | 6:+32913277 | None:intergenic |
| ACAATAGATATAAATGTTGT+TGG | 0.393513 | 6:-32913218 | MsG0680032061.01.T01:CDS |
| ACTGGTGCAGCCAGTGGAAT+TGG | 0.394448 | 6:-32913509 | MsG0680032061.01.T01:CDS |
| GGTCATAATTTGGTGGTTGA+TGG | 0.405463 | 6:-32912822 | MsG0680032061.01.T01:CDS |
| GCAGCTTCTACCGGTGGAAC+TGG | 0.415243 | 6:-32913113 | MsG0680032061.01.T01:CDS |
| CATACCATCATTGCGACGTT+AGG | 0.415601 | 6:-32913376 | MsG0680032061.01.T01:CDS |
| TAGACATTCAAGATGAATTA+GGG | 0.419372 | 6:-32913436 | MsG0680032061.01.T01:CDS |
| AAAGGTGTTGCAACTCCAAA+AGG | 0.424086 | 6:+32912990 | None:intergenic |
| ACGATCGCTTCCACAACTTC+CGG | 0.440285 | 6:+32912942 | None:intergenic |
| TCTGAAGGGTGTTGTGTTGA+AGG | 0.448691 | 6:-32912904 | MsG0680032061.01.T01:CDS |
| GGTTCATAATATAAAACAAA+TGG | 0.461191 | 6:-32912787 | MsG0680032061.01.T01:CDS |
| CATCCCTAACGTCGCAATGA+TGG | 0.461260 | 6:+32913372 | None:intergenic |
| CATCAAGTAGTAGCTTCAAT+AGG | 0.466090 | 6:-32913413 | MsG0680032061.01.T01:CDS |
| AGAAACTGTTAATTTCACTT+TGG | 0.484629 | 6:-32913336 | MsG0680032061.01.T01:CDS |
| CAAAATAGAGCCGGAAGTTG+TGG | 0.492410 | 6:-32912952 | MsG0680032061.01.T01:CDS |
| ATACCATCATTGCGACGTTA+GGG | 0.497406 | 6:-32913375 | MsG0680032061.01.T01:CDS |
| GGAATATTAGGCTCTTCATT+GGG | 0.498127 | 6:-32913275 | MsG0680032061.01.T01:CDS |
| GATATCATGTTCAGCAATGC+CGG | 0.499855 | 6:-32913296 | MsG0680032061.01.T01:CDS |
| TCTTGAACATTTAGGTTAGA+GGG | 0.503233 | 6:-32913548 | MsG0680032061.01.T01:intron |
| GCAAGCGTGGCAGCTTCTAC+CGG | 0.505412 | 6:-32913122 | MsG0680032061.01.T01:CDS |
| CATAATTTGGTGGTTGATGG+TGG | 0.514887 | 6:-32912819 | MsG0680032061.01.T01:CDS |
| CATCAGTGGTCATAATTTGG+TGG | 0.524756 | 6:-32912829 | MsG0680032061.01.T01:CDS |
| TGCCTCTTTACCAATTCCAC+TGG | 0.527579 | 6:+32913499 | None:intergenic |
| TAAGCACGCTGCTCGTGCTA+TGG | 0.528874 | 6:-32913180 | MsG0680032061.01.T01:CDS |
| TTGATCGGTGCACACGCGAA+AGG | 0.535273 | 6:+32912972 | None:intergenic |
| AAGGTGTTGCAACTCCAAAA+GGG | 0.538111 | 6:+32912991 | None:intergenic |
| TTACCAAAATGCCCCTCAGA+AGG | 0.546926 | 6:-32914503 | None:intergenic |
| GGTAGAAGCTGCCACGCTTG+CGG | 0.558287 | 6:+32913124 | None:intergenic |
| AAGTTCTAAGGGTAATCTGA+AGG | 0.564554 | 6:-32912919 | MsG0680032061.01.T01:CDS |
| GATGAAGCTGTTTACATCAG+TGG | 0.575837 | 6:-32912843 | MsG0680032061.01.T01:CDS |
| ACCACTAGGACCAGTTCCAC+CGG | 0.583291 | 6:+32913103 | None:intergenic |
| GAAGCGATCGTAAGTTCTAA+GGG | 0.587496 | 6:-32912930 | MsG0680032061.01.T01:CDS |
| AGCCAGTGGAATTGGTAAAG+AGG | 0.589227 | 6:-32913501 | MsG0680032061.01.T01:CDS |
| TGCACCGATCAAAATAGAGC+CGG | 0.591663 | 6:-32912961 | MsG0680032061.01.T01:CDS |
| GGTAAGATAGCAATCATCAC+TGG | 0.605060 | 6:-32913527 | MsG0680032061.01.T01:CDS |
| ATCATCACTGGTGCAGCCAG+TGG | 0.607127 | 6:-32913515 | MsG0680032061.01.T01:CDS |
| AGAGCAGCATGTAGTGAACT+TGG | 0.609647 | 6:-32913044 | MsG0680032061.01.T01:CDS |
| AGCGTGGCAGCTTCTACCGG+TGG | 0.610387 | 6:-32913119 | MsG0680032061.01.T01:CDS |
| AGTTCTAAGGGTAATCTGAA+GGG | 0.611602 | 6:-32912918 | MsG0680032061.01.T01:CDS |
| ACCGGTGGAACTGGTCCTAG+TGG | 0.613286 | 6:-32913104 | MsG0680032061.01.T01:CDS |
| TACCAAAATGCCCCTCAGAA+GGG | 0.620664 | 6:-32914502 | None:intergenic |
| GATCATATGCACCGCAAGCG+TGG | 0.623818 | 6:-32913135 | MsG0680032061.01.T01:CDS |
| GGTTGATGGTGGCTTCTCTG+TGG | 0.628769 | 6:-32912808 | MsG0680032061.01.T01:CDS |
| AGGTGTTGCAACTCCAAAAG+GGG | 0.636439 | 6:+32912992 | None:intergenic |
| TTTGATGCAGTATAACCACT+AGG | 0.646200 | 6:+32913089 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAGATATTTCTAAAAAT+AGG | + | Chr6:32913263-32913282 | None:intergenic | 10.0% |
| !! | ATTATTTATGAGATAACTAA+TGG | + | Chr6:32913133-32913152 | None:intergenic | 15.0% |
| !! | TAAATTAAAATAACGAAATC+TGG | - | Chr6:32913172-32913191 | MsG0680032061.01.T01:CDS | 15.0% |
| !! | TTATTTATGAGATAACTAAT+GGG | + | Chr6:32913132-32913151 | None:intergenic | 15.0% |
| !!! | ACTATACAAAAATTTGTTTA+AGG | + | Chr6:32912856-32912875 | None:intergenic | 15.0% |
| !!! | CTATACAAAAATTTGTTTAA+GGG | + | Chr6:32912855-32912874 | None:intergenic | 15.0% |
| !!! | TACTTTTCTTTATTTATCTT+TGG | - | Chr6:32913102-32913121 | MsG0680032061.01.T01:CDS | 15.0% |
| !! | ACAATAGATATAAATGTTGT+TGG | - | Chr6:32914059-32914078 | MsG0680032061.01.T01:intron | 20.0% |
| !! | GGTTCATAATATAAAACAAA+TGG | - | Chr6:32914490-32914509 | MsG0680032061.01.T01:intron | 20.0% |
| !! | GTATAGTAATGTTATATTGT+TGG | - | Chr6:32912869-32912888 | MsG0680032061.01.T01:CDS | 20.0% |
| !! | TCAAGAAAAACTAATCTAAA+GGG | + | Chr6:32913637-32913656 | None:intergenic | 20.0% |
| !! | TGACAAATCAAATATTAAGT+TGG | - | Chr6:32913222-32913241 | MsG0680032061.01.T01:CDS | 20.0% |
| !! | TTCAAGAAAAACTAATCTAA+AGG | + | Chr6:32913638-32913657 | None:intergenic | 20.0% |
| !!! | TTTTTCTTTCTTGAACATTT+AGG | - | Chr6:32913721-32913740 | MsG0680032061.01.T01:intron | 20.0% |
| ! | AGAAACTGTTAATTTCACTT+TGG | - | Chr6:32913941-32913960 | MsG0680032061.01.T01:intron | 25.0% |
| ! | AGATACACACACATATATAT+AGG | + | Chr6:32913592-32913611 | None:intergenic | 25.0% |
| ! | CAAGAAAAACTAATCTAAAG+GGG | + | Chr6:32913636-32913655 | None:intergenic | 25.0% |
| ! | GATACACACACATATATATA+GGG | + | Chr6:32913591-32913610 | None:intergenic | 25.0% |
| ! | TAGACATTCAAGATGAATTA+GGG | - | Chr6:32913841-32913860 | MsG0680032061.01.T01:intron | 25.0% |
| ! | TAGCATCAAACAAATTTACA+GGG | - | Chr6:32913531-32913550 | MsG0680032061.01.T01:CDS | 25.0% |
| ! | TATAGTTATGTATAAGTCCA+TGG | - | Chr6:32913280-32913299 | MsG0680032061.01.T01:CDS | 25.0% |
| ! | TCCATGAAAACACTATAAAT+AGG | - | Chr6:32913426-32913445 | MsG0680032061.01.T01:CDS | 25.0% |
| ! | TTAAATACGTGTTTGAAGAT+TGG | + | Chr6:32913376-32913395 | None:intergenic | 25.0% |
| ! | TTAGCATCAAACAAATTTAC+AGG | - | Chr6:32913530-32913549 | MsG0680032061.01.T01:CDS | 25.0% |
| ! | TTATCAACCTTGAAAGATAA+AGG | - | Chr6:32913474-32913493 | MsG0680032061.01.T01:CDS | 25.0% |
| !! | AATTTCACTTTGGAAAAACA+TGG | - | Chr6:32913951-32913970 | MsG0680032061.01.T01:intron | 25.0% |
| !! | GATTATCAACGATATTTTGT+AGG | + | Chr6:32913024-32913043 | None:intergenic | 25.0% |
| !! | TCCTATTTATAGTGTTTTCA+TGG | + | Chr6:32913430-32913449 | None:intergenic | 25.0% |
| !!! | ATATGTGTGTGTATCTTTTT+TGG | - | Chr6:32913596-32913615 | MsG0680032061.01.T01:intron | 25.0% |
| !!! | CAAATCAAATATTAAGTTGG+TGG | - | Chr6:32913225-32913244 | MsG0680032061.01.T01:CDS | 25.0% |
| GTAGACATTCAAGATGAATT+AGG | - | Chr6:32913840-32913859 | MsG0680032061.01.T01:intron | 30.0% | |
| TGTGTAACCTTTATCTTTCA+AGG | + | Chr6:32913484-32913503 | None:intergenic | 30.0% | |
| TTACATCAGTGGTCATAATT+TGG | - | Chr6:32914445-32914464 | MsG0680032061.01.T01:intron | 30.0% | |
| ! | TCTAGCAATTTGTTTAACCA+TGG | + | Chr6:32913300-32913319 | None:intergenic | 30.0% |
| ! | TCTTGAACATTTAGGTTAGA+GGG | - | Chr6:32913729-32913748 | MsG0680032061.01.T01:intron | 30.0% |
| ! | TTCTTGAACATTTAGGTTAG+AGG | - | Chr6:32913728-32913747 | MsG0680032061.01.T01:intron | 30.0% |
| AAGATTGGAAGACACATATG+TGG | + | Chr6:32913361-32913380 | None:intergenic | 35.0% | |
| CAATGAAGAGCCTAATATTC+CGG | + | Chr6:32914003-32914022 | None:intergenic | 35.0% | |
| CATCAAGTAGTAGCTTCAAT+AGG | - | Chr6:32913864-32913883 | MsG0680032061.01.T01:intron | 35.0% | |
| GGAATATTAGGCTCTTCATT+GGG | - | Chr6:32914002-32914021 | MsG0680032061.01.T01:intron | 35.0% | |
| GGGCAAAATGGTAATTTCAA+TGG | - | Chr6:32912795-32912814 | MsG0680032061.01.T01:CDS | 35.0% | |
| TACCTTTGCTTCATCATCAA+CGG | + | Chr6:32913567-32913586 | None:intergenic | 35.0% | |
| TGTAGTGAACTTGGTTGTTA+TGG | - | Chr6:32914242-32914261 | MsG0680032061.01.T01:intron | 35.0% | |
| TTTGATGCAGTATAACCACT+AGG | + | Chr6:32914191-32914210 | None:intergenic | 35.0% | |
| ! | GCATCAAAACATGCTCTTTT+GGG | - | Chr6:32914203-32914222 | MsG0680032061.01.T01:intron | 35.0% |
| ! | GTTAATAGTGTTTCCCCTTT+TGG | - | Chr6:32914272-32914291 | MsG0680032061.01.T01:intron | 35.0% |
| ! | TGCATCAAAACATGCTCTTT+TGG | - | Chr6:32914202-32914221 | MsG0680032061.01.T01:intron | 35.0% |
| !! | AAGTTCTAAGGGTAATCTGA+AGG | - | Chr6:32914358-32914377 | MsG0680032061.01.T01:intron | 35.0% |
| !! | AGTTCTAAGGGTAATCTGAA+GGG | - | Chr6:32914359-32914378 | MsG0680032061.01.T01:intron | 35.0% |
| AATAGGATGTACCTCTCTCT+AGG | - | Chr6:32913443-32913462 | MsG0680032061.01.T01:CDS | 40.0% | |
| ATACCATCATTGCGACGTTA+GGG | - | Chr6:32913902-32913921 | MsG0680032061.01.T01:intron | 40.0% | |
| ATGGTTGCCAAAAATGTTCG+TGG | - | Chr6:32914116-32914135 | MsG0680032061.01.T01:intron | 40.0% | |
| ATGTGGTGGCATCAAGTAAA+AGG | + | Chr6:32913344-32913363 | None:intergenic | 40.0% | |
| ATTGGAAGACACATATGTGG+TGG | + | Chr6:32913358-32913377 | None:intergenic | 40.0% | |
| CATCAGTGGTCATAATTTGG+TGG | - | Chr6:32914448-32914467 | MsG0680032061.01.T01:intron | 40.0% | |
| CGGAATATTAGGCTCTTCAT+TGG | - | Chr6:32914001-32914020 | MsG0680032061.01.T01:intron | 40.0% | |
| GATATCATGTTCAGCAATGC+CGG | - | Chr6:32913981-32914000 | MsG0680032061.01.T01:intron | 40.0% | |
| GATGAAGCTGTTTACATCAG+TGG | - | Chr6:32914434-32914453 | MsG0680032061.01.T01:intron | 40.0% | |
| GGTAAGATAGCAATCATCAC+TGG | - | Chr6:32913750-32913769 | MsG0680032061.01.T01:intron | 40.0% | |
| GTGAAGTTGTTCGTCGAAAA+TGG | - | Chr6:32913801-32913820 | MsG0680032061.01.T01:intron | 40.0% | |
| TAATAGAGAGACCTAGAGAG+AGG | + | Chr6:32913457-32913476 | None:intergenic | 40.0% | |
| TTCAGCAATGCCGGAATATT+AGG | - | Chr6:32913990-32914009 | MsG0680032061.01.T01:intron | 40.0% | |
| ! | ATTACCATTTTGCCCTTCTG+AGG | + | Chr6:32912790-32912809 | None:intergenic | 40.0% |
| ! | CAAAAGCATGTGACATCATG+AGG | + | Chr6:32913204-32913223 | None:intergenic | 40.0% |
| ! | GAAGCGATCGTAAGTTCTAA+GGG | - | Chr6:32914347-32914366 | MsG0680032061.01.T01:intron | 40.0% |
| ! | GATCGATCCACGAACATTTT+TGG | + | Chr6:32914126-32914145 | None:intergenic | 40.0% |
| ! | GGTCATAATTTGGTGGTTGA+TGG | - | Chr6:32914455-32914474 | MsG0680032061.01.T01:intron | 40.0% |
| ! | TTACCATTTTGCCCTTCTGA+GGG | + | Chr6:32912789-32912808 | None:intergenic | 40.0% |
| !! | AAAGGTGTTGCAACTCCAAA+AGG | + | Chr6:32914290-32914309 | None:intergenic | 40.0% |
| !! | AAGGTGTTGCAACTCCAAAA+GGG | + | Chr6:32914289-32914308 | None:intergenic | 40.0% |
| !! | CATAATTTGGTGGTTGATGG+TGG | - | Chr6:32914458-32914477 | MsG0680032061.01.T01:intron | 40.0% |
| !!! | ACTTCCGGCTCTATTTTGAT+CGG | + | Chr6:32914323-32914342 | None:intergenic | 40.0% |
| AGAGCAGCATGTAGTGAACT+TGG | - | Chr6:32914233-32914252 | MsG0680032061.01.T01:intron | 45.0% | |
| AGCCAGTGGAATTGGTAAAG+AGG | - | Chr6:32913776-32913795 | MsG0680032061.01.T01:intron | 45.0% | |
| CAAAATAGAGCCGGAAGTTG+TGG | - | Chr6:32914325-32914344 | MsG0680032061.01.T01:intron | 45.0% | |
| CATACCATCATTGCGACGTT+AGG | - | Chr6:32913901-32913920 | MsG0680032061.01.T01:intron | 45.0% | |
| CGACGTTAGGGATGAAAAAC+AGG | - | Chr6:32913914-32913933 | MsG0680032061.01.T01:intron | 45.0% | |
| GGAAGCGATCGTAAGTTCTA+AGG | - | Chr6:32914346-32914365 | MsG0680032061.01.T01:intron | 45.0% | |
| GGTGGCATCAAGTAAAAGGA+GGG | + | Chr6:32913340-32913359 | None:intergenic | 45.0% | |
| TGCCTCTTTACCAATTCCAC+TGG | + | Chr6:32913781-32913800 | None:intergenic | 45.0% | |
| TGGTGGCATCAAGTAAAAGG+AGG | + | Chr6:32913341-32913360 | None:intergenic | 45.0% | |
| ! | AGGTGTTGCAACTCCAAAAG+GGG | + | Chr6:32914288-32914307 | None:intergenic | 45.0% |
| ! | TACCATTTTGCCCTTCTGAG+GGG | + | Chr6:32912788-32912807 | None:intergenic | 45.0% |
| ! | TGCACCGATCAAAATAGAGC+CGG | - | Chr6:32914316-32914335 | MsG0680032061.01.T01:intron | 45.0% |
| !! | CACCGTTGATGATGAAGCAA+AGG | - | Chr6:32913562-32913581 | MsG0680032061.01.T01:intron | 45.0% |
| !! | TCTGAAGGGTGTTGTGTTGA+AGG | - | Chr6:32914373-32914392 | MsG0680032061.01.T01:intron | 45.0% |
| !! | TTATAAAATATAAATATTTG+TGG | + | Chr6:32912945-32912964 | None:intergenic | 5.0% |
| ACGATCGCTTCCACAACTTC+CGG | + | Chr6:32914338-32914357 | None:intergenic | 50.0% | |
| CATCCCTAACGTCGCAATGA+TGG | + | Chr6:32913908-32913927 | None:intergenic | 50.0% | |
| ACCACTAGGACCAGTTCCAC+CGG | + | Chr6:32914177-32914196 | None:intergenic | 55.0% | |
| ACTGGTGCAGCCAGTGGAAT+TGG | - | Chr6:32913768-32913787 | MsG0680032061.01.T01:intron | 55.0% | |
| ATCATCACTGGTGCAGCCAG+TGG | - | Chr6:32913762-32913781 | MsG0680032061.01.T01:intron | 55.0% | |
| GATCATATGCACCGCAAGCG+TGG | - | Chr6:32914142-32914161 | MsG0680032061.01.T01:intron | 55.0% | |
| TAAGCACGCTGCTCGTGCTA+TGG | - | Chr6:32914097-32914116 | MsG0680032061.01.T01:intron | 55.0% | |
| TGCCCCTCAGAAGGGCAAAA+TGG | - | Chr6:32912783-32912802 | MsG0680032061.01.T01:CDS | 55.0% | |
| TTGATCGGTGCACACGCGAA+AGG | + | Chr6:32914308-32914327 | None:intergenic | 55.0% | |
| !! | GGTTGATGGTGGCTTCTCTG+TGG | - | Chr6:32914469-32914488 | MsG0680032061.01.T01:intron | 55.0% |
| ACCGGTGGAACTGGTCCTAG+TGG | - | Chr6:32914173-32914192 | MsG0680032061.01.T01:intron | 60.0% | |
| GCAAGCGTGGCAGCTTCTAC+CGG | - | Chr6:32914155-32914174 | MsG0680032061.01.T01:intron | 60.0% | |
| GCAGCTTCTACCGGTGGAAC+TGG | - | Chr6:32914164-32914183 | MsG0680032061.01.T01:intron | 60.0% | |
| GGTAGAAGCTGCCACGCTTG+CGG | + | Chr6:32914156-32914175 | None:intergenic | 60.0% | |
| AGCGTGGCAGCTTCTACCGG+TGG | - | Chr6:32914158-32914177 | MsG0680032061.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 32912782 | 32914517 | 32912782 | ID=MsG0680032061.01;Name=MsG0680032061.01 |
| Chr6 | mRNA | 32912782 | 32914517 | 32912782 | ID=MsG0680032061.01.T01;Parent=MsG0680032061.01;Name=MsG0680032061.01.T01;_AED=0.31;_eAED=0.31;_QI=0|0|0|0.5|1|1|2|0|265 |
| Chr6 | exon | 32914495 | 32914517 | 32914495 | ID=MsG0680032061.01.T01:exon:18213;Parent=MsG0680032061.01.T01 |
| Chr6 | exon | 32912782 | 32913556 | 32912782 | ID=MsG0680032061.01.T01:exon:18212;Parent=MsG0680032061.01.T01 |
| Chr6 | CDS | 32914495 | 32914517 | 32914495 | ID=MsG0680032061.01.T01:cds;Parent=MsG0680032061.01.T01 |
| Chr6 | CDS | 32912782 | 32913556 | 32912782 | ID=MsG0680032061.01.T01:cds;Parent=MsG0680032061.01.T01 |
| Gene Sequence |
| Protein sequence |
