AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004101.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004101.01.T01 MTR_1g075170 71.429 63 18 0 1 63 1 63 6.84e-22 92.4
MsG0180004101.01.T01 MTR_1g075170 78.182 55 11 1 126 179 330 384 6.14e-21 89.7
MsG0180004101.01.T01 MTR_1g075170 56.061 66 27 1 82 145 192 257 4.78e-16 75.5
MsG0180004101.01.T01 MTR_1g040360 60.000 55 21 1 126 180 343 396 7.09e-13 66.2
MsG0180004101.01.T01 MTR_1g075153 70.455 44 12 1 130 173 329 371 3.37e-12 64.3
MsG0180004101.01.T01 MTR_1g075180 64.815 54 14 3 128 180 304 353 4.49e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGGAGGTACCATTCTTT+TGG 0.182584 1:-73362541 None:intergenic
ATCAATATCGTCAAGCTTAA+AGG 0.190937 1:-73362588 None:intergenic
GGATTCCCATTGTGGATAAT+TGG 0.226633 1:+73365191 MsG0180004101.01.T01:CDS
GCCATTGATTGGTACATCTT+TGG 0.251729 1:-73362510 None:intergenic
TCTGCCTCAACTCAGATTTC+TGG 0.271962 1:+73362697 MsG0180004101.01.T01:CDS
TGGATTCTCTTGTTGTGTTA+TGG 0.321584 1:+73363299 MsG0180004101.01.T01:CDS
GGAGCTGAGATTTCTGTTCT+TGG 0.330206 1:-73362559 None:intergenic
AAACCACAAGAAGTGATAAA+AGG 0.343304 1:+73365139 MsG0180004101.01.T01:CDS
TCGTCAAGCTTAAAGGGATT+AGG 0.343557 1:-73362581 None:intergenic
CTACCATTGTTTGCCTGTAC+TGG 0.344239 1:+73363329 MsG0180004101.01.T01:CDS
GAGCTGAGATTTCTGTTCTT+GGG 0.379012 1:-73362558 None:intergenic
CGTCAAGCTTAAAGGGATTA+GGG 0.380837 1:-73362580 None:intergenic
GGGGCAGCATGGTCAGCTTT+CGG 0.411532 1:+73363233 MsG0180004101.01.T01:CDS
GCCTGTACTGGTAACCTTGT+CGG 0.419441 1:+73363341 MsG0180004101.01.T01:CDS
TGGGTCGTAGTGCCATTGAT+TGG 0.424583 1:-73362521 None:intergenic
GGGTGATATAAACTTTGTTA+AGG 0.430161 1:-73362613 None:intergenic
TCAATATCGTCAAGCTTAAA+GGG 0.433331 1:-73362587 None:intergenic
GATAAAAGGTTTCAAGAAAG+AGG 0.440536 1:+73365153 MsG0180004101.01.T01:CDS
GTCAGCTTTCGGTTATGACT+TGG 0.459956 1:+73363244 MsG0180004101.01.T01:CDS
TGTTGGAGACAAGCTTGAAA+AGG 0.463060 1:-73362669 None:intergenic
CACCCATGTCAAAGATTATG+AGG 0.466569 1:+73362631 MsG0180004101.01.T01:CDS
GATTCCCATTGTGGATAATT+GGG 0.467568 1:+73365192 MsG0180004101.01.T01:CDS
ATTTCGAGATTAGACAGCAT+TGG 0.478991 1:+73365070 MsG0180004101.01.T01:intron
TCATCCCAATTATCCACAAT+GGG 0.497534 1:-73365196 None:intergenic
TCCGACAAGGTTACCAGTAC+AGG 0.500684 1:-73363342 None:intergenic
AGAATCCATTGCATAGCATC+AGG 0.501216 1:-73363284 None:intergenic
TCCAAAGATGTACCAATCAA+TGG 0.506446 1:+73362509 MsG0180004101.01.T01:CDS
GTTGGAGACAAGCTTGAAAA+GGG 0.520637 1:-73362668 None:intergenic
AGTTGAGGCAGAGAGGATGT+TGG 0.533320 1:-73362686 None:intergenic
GGCACTACGACCCAAAAGAA+TGG 0.536228 1:+73362530 MsG0180004101.01.T01:CDS
CTGAGATTTCTGTTCTTGGG+AGG 0.538881 1:-73362555 None:intergenic
TTCCTCATAATCTTTGACAT+GGG 0.560884 1:-73362633 None:intergenic
ATTCCTCATAATCTTTGACA+TGG 0.565424 1:-73362634 None:intergenic
TTCATCCCAATTATCCACAA+TGG 0.566307 1:-73365197 None:intergenic
CTTTGCCTGATGCTATGCAA+TGG 0.584464 1:+73363279 MsG0180004101.01.T01:CDS
CATTAAACAGTGGGGCAGCA+TGG 0.590489 1:+73363222 MsG0180004101.01.T01:intron
AGTTCCAGAAATCTGAGTTG+AGG 0.604866 1:-73362701 None:intergenic
TACATCTTTGGACAATGCAG+TGG 0.608358 1:-73362498 None:intergenic
TTTGGACAATGCAGTGGACA+TGG 0.617395 1:-73362492 None:intergenic
AAATCTGAGTTGAGGCAGAG+AGG 0.625519 1:-73362693 None:intergenic
TTACCAGTACAGGCAAACAA+TGG 0.693046 1:-73363332 None:intergenic
AGAACTTACGATATCCGACA+AGG 0.722231 1:-73363355 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAA+TGG - Chr1:73364094-73364075 None:intergenic 0.0%
!! ATATTATATATATATATATA+GGG + Chr1:73363835-73363854 MsG0180004101.01.T01:intron 0.0%
!! TATATATATATATATATAAT+GGG - Chr1:73364095-73364076 None:intergenic 0.0%
!! TATATTATATATATATATAT+AGG + Chr1:73363834-73363853 MsG0180004101.01.T01:intron 0.0%
!! TATTATCAGATTATAAAAAA+AGG + Chr1:73363471-73363490 MsG0180004101.01.T01:intron 10.0%
!! AAATGCTAATTACTATATAT+TGG + Chr1:73363618-73363637 MsG0180004101.01.T01:intron 15.0%
!! ATAAAAAAAAAAAACTCCAT+CGG + Chr1:73362799-73362818 MsG0180004101.01.T01:intron 15.0%
!! TAAAAGATAACATAGTAAAA+GGG - Chr1:73364985-73365004 None:intergenic 15.0%
!! TAGTATATGAAGAAAAAATT+GGG - Chr1:73364776-73364795 None:intergenic 15.0%
!!! TTGACATTCTTTTTTATTTT+AGG - Chr1:73364337-73364356 None:intergenic 15.0%
!!! TTTTATTAATCTGTATGAAA+TGG + Chr1:73362873-73362892 MsG0180004101.01.T01:intron 15.0%
!! AATCTGAGTACAATTATATT+GGG - Chr1:73364380-73364399 None:intergenic 20.0%
!! AGTAAATTTCATTAACATGT+CGG - Chr1:73364533-73364552 None:intergenic 20.0%
!! CACATGAAAATTAATCTTAA+TGG - Chr1:73364479-73364498 None:intergenic 20.0%
!! CTAGTATATGAAGAAAAAAT+TGG - Chr1:73364777-73364796 None:intergenic 20.0%
!! GTAAAAGATAACATAGTAAA+AGG - Chr1:73364986-73365005 None:intergenic 20.0%
!! GTACATAAATAATACATATG+TGG + Chr1:73363553-73363572 MsG0180004101.01.T01:intron 20.0%
!! TATATACATGTAAGTGTAAA+TGG + Chr1:73363423-73363442 MsG0180004101.01.T01:intron 20.0%
!! TATTCTTACTTGGTATAAAA+AGG + Chr1:73364848-73364867 MsG0180004101.01.T01:intron 20.0%
!! TCTTGTAAATGAATAAAACA+TGG + Chr1:73364425-73364444 MsG0180004101.01.T01:intron 20.0%
!! TGAAATCATATCATTCAAAA+TGG - Chr1:73364613-73364632 None:intergenic 20.0%
!!! ATCAAATACACCAATATTTT+AGG + Chr1:73363747-73363766 MsG0180004101.01.T01:intron 20.0%
!!! CGTAATTAATTTGTTAATGT+CGG + Chr1:73362738-73362757 MsG0180004101.01.T01:intron 20.0%
!!! TGATTTAAAGAAGCTTTAAA+AGG - Chr1:73364235-73364254 None:intergenic 20.0%
! ATGTATATACGATATAGTGA+CGG - Chr1:73363412-73363431 None:intergenic 25.0%
! GAATCTGAGTACAATTATAT+TGG - Chr1:73364381-73364400 None:intergenic 25.0%
! GTAAATTTCATTAACATGTC+GGG - Chr1:73364532-73364551 None:intergenic 25.0%
! TAAATTTCATTAACATGTCG+GGG - Chr1:73364531-73364550 None:intergenic 25.0%
! TAATTATCCTTATTAACCGA+TGG - Chr1:73362818-73362837 None:intergenic 25.0%
! TACTAAATGATTACTATGCA+GGG + Chr1:73364701-73364720 MsG0180004101.01.T01:intron 25.0%
! TTACTAAATGATTACTATGC+AGG + Chr1:73364700-73364719 MsG0180004101.01.T01:intron 25.0%
! TTGGGATCACTATAAAAAAA+TGG - Chr1:73364362-73364381 None:intergenic 25.0%
! TTGTTTCATGTATTCTTACT+TGG + Chr1:73364838-73364857 MsG0180004101.01.T01:intron 25.0%
!! AATGGTTCTTTTATTCTTGT+AGG + Chr1:73363441-73363460 MsG0180004101.01.T01:intron 25.0%
!! ATGGTTCTTTTATTCTTGTA+GGG + Chr1:73363442-73363461 MsG0180004101.01.T01:intron 25.0%
!! ATGTATTCATGTGTTTTACA+AGG - Chr1:73364677-73364696 None:intergenic 25.0%
!!! ATATTTTAGTCACACCAATA+TGG + Chr1:73364945-73364964 MsG0180004101.01.T01:intron 25.0%
AAACCACAAGAAGTGATAAA+AGG + Chr1:73365139-73365158 MsG0180004101.01.T01:CDS 30.0%
AAAGTGAAAATCACACCTTA+AGG - Chr1:73363949-73363968 None:intergenic 30.0%
AAGATAACATAGTAAAAGGG+CGG - Chr1:73364982-73365001 None:intergenic 30.0%
AATCACGTTAGAAATTGCAT+TGG + Chr1:73364250-73364269 MsG0180004101.01.T01:intron 30.0%
AATTGGGTAATAATCTAGTG+TGG - Chr1:73364760-73364779 None:intergenic 30.0%
ACTAAATGATTACTATGCAG+GGG + Chr1:73364702-73364721 MsG0180004101.01.T01:intron 30.0%
ATCAATATCGTCAAGCTTAA+AGG - Chr1:73362591-73362610 None:intergenic 30.0%
ATCACGTTAGAAATTGCATT+GGG + Chr1:73364251-73364270 MsG0180004101.01.T01:intron 30.0%
ATTCAATCATCAGAACACTA+TGG + Chr1:73363109-73363128 MsG0180004101.01.T01:intron 30.0%
ATTCCTCATAATCTTTGACA+TGG - Chr1:73362637-73362656 None:intergenic 30.0%
CAAAATGGAAAACAGAAATC+TGG - Chr1:73364598-73364617 None:intergenic 30.0%
GATAAAAGACACCTTCATAA+AGG + Chr1:73364029-73364048 MsG0180004101.01.T01:intron 30.0%
GATAAAAGGTTTCAAGAAAG+AGG + Chr1:73365153-73365172 MsG0180004101.01.T01:CDS 30.0%
GGGTGATATAAACTTTGTTA+AGG - Chr1:73362616-73362635 None:intergenic 30.0%
GTACTCAGATTCTTTAAGTA+AGG + Chr1:73364389-73364408 MsG0180004101.01.T01:intron 30.0%
GTTACAAGACATTAAACAGT+GGG + Chr1:73363213-73363232 MsG0180004101.01.T01:intron 30.0%
TCAATATCGTCAAGCTTAAA+GGG - Chr1:73362590-73362609 None:intergenic 30.0%
TGAACTTGTCACATTCTTAT+TGG + Chr1:73363186-73363205 MsG0180004101.01.T01:intron 30.0%
TGTATATTCCCTACACAAAT+TGG - Chr1:73363646-73363665 None:intergenic 30.0%
TGTTACAAGACATTAAACAG+TGG + Chr1:73363212-73363231 MsG0180004101.01.T01:intron 30.0%
TTAAAGCACAAGAGCAAAAA+TGG - Chr1:73364885-73364904 None:intergenic 30.0%
TTACAAGACATTAAACAGTG+GGG + Chr1:73363214-73363233 MsG0180004101.01.T01:intron 30.0%
TTCCTCATAATCTTTGACAT+GGG - Chr1:73362636-73362655 None:intergenic 30.0%
! AAAAACTCCATCGGTTAATA+AGG + Chr1:73362808-73362827 MsG0180004101.01.T01:intron 30.0%
! AAACCTTTTATCACTTCTTG+TGG - Chr1:73365145-73365164 None:intergenic 30.0%
! AAGAGGATTTCAAGATTTTG+TGG + Chr1:73365170-73365189 MsG0180004101.01.T01:CDS 30.0%
! TAATTGTCAATGCCTGTTTT+TGG - Chr1:73364303-73364322 None:intergenic 30.0%
! TTTTATCAAGTCACACCTTA+AGG + Chr1:73363931-73363950 MsG0180004101.01.T01:intron 30.0%
!! ATATTGGTTCCAATTTGTGT+AGG + Chr1:73363634-73363653 MsG0180004101.01.T01:intron 30.0%
!! CTTGTTGTTGCCTAAAATAT+TGG - Chr1:73363760-73363779 None:intergenic 30.0%
!! TATTGGTTCCAATTTGTGTA+GGG + Chr1:73363635-73363654 MsG0180004101.01.T01:intron 30.0%
!!! AATTTTTTTCTTCCCAACAC+AGG + Chr1:73365010-73365029 MsG0180004101.01.T01:intron 30.0%
!!! ATTTTTTTCTTCCCAACACA+GGG + Chr1:73365011-73365030 MsG0180004101.01.T01:intron 30.0%
AGAACCTCCTAAAAGTAAGT+TGG + Chr1:73363989-73364008 MsG0180004101.01.T01:intron 35.0%
ATTTCGAGATTAGACAGCAT+TGG + Chr1:73365070-73365089 MsG0180004101.01.T01:intron 35.0%
GAACCTTTCTGAATTGAACT+TGG - Chr1:73363155-73363174 None:intergenic 35.0%
GAATAAAACATGGTTGTGAG+TGG + Chr1:73364435-73364454 MsG0180004101.01.T01:intron 35.0%
GACAATATAGTTAGACACTC+AGG - Chr1:73363801-73363820 None:intergenic 35.0%
GATAGAAGACACCTTTATGA+AGG - Chr1:73364043-73364062 None:intergenic 35.0%
GATAGACGAAAGAATCAAGA+TGG - Chr1:73362850-73362869 None:intergenic 35.0%
GATTCCCATTGTGGATAATT+GGG + Chr1:73365192-73365211 MsG0180004101.01.T01:CDS 35.0%
GGTGCAATTACGATCTTAAT+AGG + Chr1:73363086-73363105 MsG0180004101.01.T01:intron 35.0%
TAAAAAAAGGCTCTCTCATG+TGG + Chr1:73363484-73363503 MsG0180004101.01.T01:intron 35.0%
TAGCCAAGTTCAATTCAGAA+AGG + Chr1:73363149-73363168 MsG0180004101.01.T01:intron 35.0%
TCATCCCAATTATCCACAAT+GGG - Chr1:73365199-73365218 None:intergenic 35.0%
TCCAAAGATGTACCAATCAA+TGG + Chr1:73362509-73362528 MsG0180004101.01.T01:CDS 35.0%
TGACAAGTTCAGTTATTCAC+AGG - Chr1:73363177-73363196 None:intergenic 35.0%
TTAGAAATTGCATTGGGAAG+TGG + Chr1:73364257-73364276 MsG0180004101.01.T01:intron 35.0%
TTAGGCAACAACAAGTAAAC+AGG + Chr1:73363765-73363784 MsG0180004101.01.T01:intron 35.0%
TTCATCCCAATTATCCACAA+TGG - Chr1:73365200-73365219 None:intergenic 35.0%
TTGATAAAAGACACCACCAA+GGG - Chr1:73363920-73363939 None:intergenic 35.0%
TTTAGTCACACCAATATGGA+TGG + Chr1:73364949-73364968 MsG0180004101.01.T01:intron 35.0%
TTTCTTGAGACTTCTGATCA+AGG + Chr1:73363714-73363733 MsG0180004101.01.T01:intron 35.0%
! TGGATTCTCTTGTTGTGTTA+TGG + Chr1:73363299-73363318 MsG0180004101.01.T01:CDS 35.0%
!! ATGAGCACCAACTTACTTTT+AGG - Chr1:73363999-73364018 None:intergenic 35.0%
!!! AATGCCTGTTTTTGGATTCA+CGG - Chr1:73364295-73364314 None:intergenic 35.0%
AAGACTTCAATACCCTGTGT+TGG - Chr1:73365026-73365045 None:intergenic 40.0%
ACAAGTAAACAGGTTAGTGC+TGG + Chr1:73363775-73363794 MsG0180004101.01.T01:intron 40.0%
ACTTGGCTAAGTTGCTTCAA+TGG - Chr1:73363138-73363157 None:intergenic 40.0%
AGAACTTACGATATCCGACA+AGG - Chr1:73363358-73363377 None:intergenic 40.0%
AGAATCCATTGCATAGCATC+AGG - Chr1:73363287-73363306 None:intergenic 40.0%
AGACTTCAATACCCTGTGTT+GGG - Chr1:73365025-73365044 None:intergenic 40.0%
AGTTCCAGAAATCTGAGTTG+AGG - Chr1:73362704-73362723 None:intergenic 40.0%
ATGTTTGTGCAAGCGAGATT+TGG + Chr1:73364184-73364203 MsG0180004101.01.T01:intron 40.0%
CACCCATGTCAAAGATTATG+AGG + Chr1:73362631-73362650 MsG0180004101.01.T01:CDS 40.0%
CGTCAAGCTTAAAGGGATTA+GGG - Chr1:73362583-73362602 None:intergenic 40.0%
CTCATTTGGAAGTTGCAAAG+TGG + Chr1:73363524-73363543 MsG0180004101.01.T01:intron 40.0%
CTTGATAAAAGACACCACCA+AGG - Chr1:73363921-73363940 None:intergenic 40.0%
GAGCTGAGATTTCTGTTCTT+GGG - Chr1:73362561-73362580 None:intergenic 40.0%
GCACAAGAGCAAAAATGGTT+TGG - Chr1:73364880-73364899 None:intergenic 40.0%
GGAAGTTGCAAAGTGGAAAT+TGG + Chr1:73363531-73363550 MsG0180004101.01.T01:intron 40.0%
GGATTCCCATTGTGGATAAT+TGG + Chr1:73365191-73365210 MsG0180004101.01.T01:CDS 40.0%
GTTGGAGACAAGCTTGAAAA+GGG - Chr1:73362671-73362690 None:intergenic 40.0%
TAACTCTAGCTGCATCTCTT+TGG + Chr1:73363058-73363077 MsG0180004101.01.T01:intron 40.0%
TACATCTTTGGACAATGCAG+TGG - Chr1:73362501-73362520 None:intergenic 40.0%
TCGTCAAGCTTAAAGGGATT+AGG - Chr1:73362584-73362603 None:intergenic 40.0%
TGAGAATCCACATTGTTGTC+TGG - Chr1:73362999-73363018 None:intergenic 40.0%
TGTTGGAGACAAGCTTGAAA+AGG - Chr1:73362672-73362691 None:intergenic 40.0%
TTACCAGTACAGGCAAACAA+TGG - Chr1:73363335-73363354 None:intergenic 40.0%
TTGTCCGTGAATCCAAAAAC+AGG + Chr1:73364288-73364307 MsG0180004101.01.T01:intron 40.0%
! GATTTTGTGGATTCCCATTG+TGG + Chr1:73365183-73365202 MsG0180004101.01.T01:CDS 40.0%
! GCCATTGATTGGTACATCTT+TGG - Chr1:73362513-73362532 None:intergenic 40.0%
! TATCAATGCTTTTGCTTCCC+AGG - Chr1:73363040-73363059 None:intergenic 40.0%
! TTAACGAGTTGAGGCTCATT+TGG + Chr1:73363510-73363529 MsG0180004101.01.T01:intron 40.0%
! TTATGTTAGAAGCACACCCT+TGG + Chr1:73363901-73363920 MsG0180004101.01.T01:intron 40.0%
!! AGCACCAACTTACTTTTAGG+AGG - Chr1:73363996-73364015 None:intergenic 40.0%
!!! TTGGGAGGTACCATTCTTTT+GGG - Chr1:73362543-73362562 None:intergenic 40.0%
AAAAAGGCTCTCTCATGTGG+TGG + Chr1:73363487-73363506 MsG0180004101.01.T01:intron 45.0%
ATCCAGCATAAGGCATAGTG+TGG + Chr1:73364728-73364747 MsG0180004101.01.T01:intron 45.0%
ATGTGGTGGTTAACGAGTTG+AGG + Chr1:73363501-73363520 MsG0180004101.01.T01:intron 45.0%
CACACCAATATGGATGGTCT+AGG + Chr1:73364955-73364974 MsG0180004101.01.T01:intron 45.0%
CTACCATTGTTTGCCTGTAC+TGG + Chr1:73363329-73363348 MsG0180004101.01.T01:CDS 45.0%
CTGAGATTTCTGTTCTTGGG+AGG - Chr1:73362558-73362577 None:intergenic 45.0%
CTTTGCCTGATGCTATGCAA+TGG + Chr1:73363279-73363298 MsG0180004101.01.T01:CDS 45.0%
GCGAGATTTGGCAAAAACCA+CGG + Chr1:73364196-73364215 MsG0180004101.01.T01:intron 45.0%
GGAGCTGAGATTTCTGTTCT+TGG - Chr1:73362562-73362581 None:intergenic 45.0%
GTCAGCTTTCGGTTATGACT+TGG + Chr1:73363244-73363263 MsG0180004101.01.T01:CDS 45.0%
TCTGCCTCAACTCAGATTTC+TGG + Chr1:73362697-73362716 MsG0180004101.01.T01:CDS 45.0%
! AAATCTGAGTTGAGGCAGAG+AGG - Chr1:73362696-73362715 None:intergenic 45.0%
!! AGCTGCATCTCTTTGGAGTA+TGG + Chr1:73363065-73363084 MsG0180004101.01.T01:intron 45.0%
!!! CTTGGGAGGTACCATTCTTT+TGG - Chr1:73362544-73362563 None:intergenic 45.0%
AGCCACACTATGCCTTATGC+TGG - Chr1:73364733-73364752 None:intergenic 50.0%
AGTTGAGGCAGAGAGGATGT+TGG - Chr1:73362689-73362708 None:intergenic 50.0%
ATCACCTCAGAGACTTCTCC+TGG + Chr1:73363019-73363038 MsG0180004101.01.T01:intron 50.0%
CATTAAACAGTGGGGCAGCA+TGG + Chr1:73363222-73363241 MsG0180004101.01.T01:intron 50.0%
CGGACCTAGACCATCCATAT+TGG - Chr1:73364962-73364981 None:intergenic 50.0%
GGCACTACGACCCAAAAGAA+TGG + Chr1:73362530-73362549 MsG0180004101.01.T01:CDS 50.0%
GTGCACACCAGACAACAATG+TGG + Chr1:73362989-73363008 MsG0180004101.01.T01:intron 50.0%
TCACCTCAGAGACTTCTCCT+GGG + Chr1:73363020-73363039 MsG0180004101.01.T01:intron 50.0%
TCCGACAAGGTTACCAGTAC+AGG - Chr1:73363345-73363364 None:intergenic 50.0%
! AAGGTAGTTCTGCTGAACCG+TGG - Chr1:73364216-73364235 None:intergenic 50.0%
! GCCTGTACTGGTAACCTTGT+CGG + Chr1:73363341-73363360 MsG0180004101.01.T01:CDS 50.0%
! TGACAGATGATAACGACCCG+CGG + Chr1:73362955-73362974 MsG0180004101.01.T01:intron 50.0%
! TGGGTCGTAGTGCCATTGAT+TGG - Chr1:73362524-73362543 None:intergenic 50.0%
! TGTTAGAAGCACACCCTTGG+TGG + Chr1:73363904-73363923 MsG0180004101.01.T01:intron 50.0%
CTTCCCAGGAGAAGTCTCTG+AGG - Chr1:73363026-73363045 None:intergenic 55.0%
GGGGCAGCATGGTCAGCTTT+CGG + Chr1:73363233-73363252 MsG0180004101.01.T01:CDS 60.0%
GTGCACATTCTGCTGTGCCG+CGG - Chr1:73362975-73362994 None:intergenic 60.0%
TGCACATTCTGCTGTGCCGC+GGG - Chr1:73362974-73362993 None:intergenic 60.0%
!! GCAGGGGAGCATCCAGCATA+AGG + Chr1:73364718-73364737 MsG0180004101.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 73362494 73365246 73362494 ID=MsG0180004101.01;Name=MsG0180004101.01
Chr1 mRNA 73362494 73365246 73362494 ID=MsG0180004101.01.T01;Parent=MsG0180004101.01;Name=MsG0180004101.01.T01;_AED=0.50;_eAED=0.55;_QI=0|0|0|1|1|1|3|0|181
Chr1 exon 73362494 73362738 73362494 ID=MsG0180004101.01.T01:exon:5271;Parent=MsG0180004101.01.T01
Chr1 exon 73363232 73363368 73363232 ID=MsG0180004101.01.T01:exon:5272;Parent=MsG0180004101.01.T01
Chr1 exon 73365083 73365246 73365083 ID=MsG0180004101.01.T01:exon:5273;Parent=MsG0180004101.01.T01
Chr1 CDS 73362494 73362738 73362494 ID=MsG0180004101.01.T01:cds;Parent=MsG0180004101.01.T01
Chr1 CDS 73363232 73363368 73363232 ID=MsG0180004101.01.T01:cds;Parent=MsG0180004101.01.T01
Chr1 CDS 73365083 73365246 73365083 ID=MsG0180004101.01.T01:cds;Parent=MsG0180004101.01.T01
Gene Sequence

>MsG0180004101.01.T01

ATGTCCACTGCATTGTCCAAAGATGTACCAATCAATGGCACTACGACCCAAAAGAATGGTACCTCCCAAGAACAGAAATCTCAGCTCCCTAATCCCTTTAAGCTTGACGATATTGATATCCTTAACAAAGTTTATATCACCCATGTCAAAGATTATGAGGAATATGATAATGAACCCTTTTCAAGCTTGTCTCCAACATCCTCTCTGCCTCAACTCAGATTTCTGGAACTAATTCTGCACTTAACTGGGGCAGCATGGTCAGCTTTCGGTTATGACTTGGACGCTGTTCATTCTTTGCCTGATGCTATGCAATGGATTCTCTTGTTGTGTTATGGACAGTTGCTACCATTGTTTGCCTGTACTGGTAACCTTGTCGGATATCACAGCATTGGAGATGAGATTTTGATGTTAAATTCAATCTATAAAAGATTGCAAGACAAACCACAAGAAGTGATAAAAGGTTTCAAGAAAGAGGATTTCAAGATTTTGTGGATTCCCATTGTGGATAATTGGGATGAAGACAGCAAGAATGAGTTTAATATTTGA
Protein sequence

>MsG0180004101.01.T01

MSTALSKDVPINGTTTQKNGTSQEQKSQLPNPFKLDDIDILNKVYITHVKDYEEYDNEPFSSLSPTSSLPQLRFLELILHLTGAAWSAFGYDLDAVHSLPDAMQWILLLCYGQLLPLFACTGNLVGYHSIGDEILMLNSIYKRLQDKPQEVIKGFKKEDFKILWIPIVDNWDEDSKNEFNI*