AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029825.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029825.01.T01 MTR_5g093520 92.683 82 6 0 50 131 227 308 6.06e-49 162
MsG0580029825.01.T01 MTR_5g093520 80.851 47 9 0 3 49 85 131 7.82e-19 81.3
MsG0580029825.01.T01 MTR_7g051910 42.647 136 63 3 10 132 154 287 1.46e-26 102
MsG0580029825.01.T01 MTR_2g026080 50.602 83 40 1 49 131 211 292 1.22e-24 97.4
MsG0580029825.01.T01 MTR_4g133620 34.351 131 81 3 5 132 162 290 2.10e-20 85.5
MsG0580029825.01.T01 MTR_8g009880 47.561 82 42 1 50 131 213 293 3.59e-17 76.6
MsG0580029825.01.T01 MTR_5g097620 46.341 82 43 1 50 131 253 333 7.27e-16 73.2
MsG0580029825.01.T01 MTR_8g028075 42.857 84 43 2 49 132 399 477 1.36e-15 72.4
MsG0580029825.01.T01 MTR_3g058510 39.759 83 49 1 50 132 209 290 1.72e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029825.01.T01 AT3G25290 51.111 90 42 2 44 132 212 300 2.54e-24 96.3
MsG0580029825.01.T01 AT3G25290 51.111 90 42 2 44 132 212 300 2.54e-24 96.3
MsG0580029825.01.T01 AT4G12980 46.429 84 44 1 49 132 219 301 6.04e-23 92.8
MsG0580029825.01.T01 AT5G35735 48.810 84 40 1 49 132 209 289 6.16e-22 90.1
MsG0580029825.01.T01 AT4G17280 47.619 84 43 1 49 132 217 299 1.43e-21 89.0
MsG0580029825.01.T01 AT5G47530 47.619 84 43 1 49 132 209 291 1.95e-21 88.6
MsG0580029825.01.T01 AT2G04850 42.105 95 54 1 35 129 203 296 2.85e-17 77.0
MsG0580029825.01.T01 AT3G07570 38.554 83 47 1 49 131 211 289 3.13e-13 65.5
MsG0580029825.01.T01 AT3G61750 44.444 81 40 2 51 131 220 295 4.37e-13 65.1
MsG0580029825.01.T01 AT3G61750 44.444 81 40 2 51 131 143 218 4.84e-13 64.7
MsG0580029825.01.T01 AT3G59070 34.862 109 57 5 10 105 169 276 2.24e-11 60.5

Find 46 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACTGCTGGTTGGGCTAT+TGG 0.222937 5:-102483216 MsG0580029825.01.T01:CDS
TGGGCTATTGGATTGGTTCT+TGG 0.243224 5:-102483204 MsG0580029825.01.T01:CDS
CATGGAGTTCTAAACATTAT+AGG 0.297798 5:-102483360 MsG0580029825.01.T01:CDS
TGCCAAAGGTTCGATGAGTT+TGG 0.326228 5:+102483157 None:intergenic
TCTCATTGGTACTGCTGGTT+GGG 0.341554 5:-102483223 MsG0580029825.01.T01:CDS
CACATTGGTTGCCAAACAAT+TGG 0.361854 5:-102483249 MsG0580029825.01.T01:CDS
ATTGGAACAAAGGCAATCAT+AGG 0.380791 5:-102483999 MsG0580029825.01.T01:CDS
TGCTGGTTGGGCTATTGGAT+TGG 0.383851 5:-102483211 MsG0580029825.01.T01:CDS
TGGTTCCTTAGTAACATCAT+AGG 0.384440 5:+102483941 None:intergenic
CAATGAGAAAACCAATTGTT+TGG 0.385134 5:+102483238 None:intergenic
TTCCAAACTCATCGAACCTT+TGG 0.390160 5:-102483159 MsG0580029825.01.T01:CDS
TTCTCATTGGTACTGCTGGT+TGG 0.395802 5:-102483224 MsG0580029825.01.T01:CDS
ATCATTTCAGCCCTTTCCCC+TGG 0.395994 5:+102484020 None:intergenic
TAAAAGCCTCCAACCCTGTT+TGG 0.400789 5:+102483921 None:intergenic
GGGGAAAGGGCTGAAATGAT+TGG 0.432198 5:-102484017 MsG0580029825.01.T01:CDS
ATGGAGTTCTAAACATTATA+GGG 0.438643 5:-102483359 MsG0580029825.01.T01:CDS
ATGTTACTAAGGAACCAAAC+AGG 0.440334 5:-102483935 MsG0580029825.01.T01:CDS
TGTATTGAAATTTAGGTACA+TGG 0.442348 5:-102483378 MsG0580029825.01.T01:intron
GGATACACTCGAAAGTATCT+TGG 0.442467 5:+102483303 None:intergenic
GGGTGTAAATCCAGGGGAAA+GGG 0.460004 5:-102484030 MsG0580029825.01.T01:CDS
GTGGGGAACATTGTTACCTA+TGG 0.468400 5:-102483337 MsG0580029825.01.T01:CDS
AAGGGGATGGGTGGATAGCA+TGG 0.470518 5:-102484052 MsG0580029825.01.T01:CDS
ATAGCATGGGGTGTAAATCC+AGG 0.477364 5:-102484038 MsG0580029825.01.T01:CDS
GCCAATATGGATCAAGGGGA+TGG 0.480674 5:-102484065 None:intergenic
TTCGGTGCCAATATGGATCA+AGG 0.487774 5:-102484071 None:intergenic
CTTGGAATTATTACTCCCAT+AGG 0.497045 5:+102483321 None:intergenic
TAAAACCTATGATGTTACTA+AGG 0.510200 5:-102483946 MsG0580029825.01.T01:CDS
AGGGGATGGGTGGATAGCAT+GGG 0.510652 5:-102484051 MsG0580029825.01.T01:CDS
GGGGTGTAAATCCAGGGGAA+AGG 0.530797 5:-102484031 MsG0580029825.01.T01:CDS
ATCATAGGGATCAAATATCA+TGG 0.537405 5:-102483984 MsG0580029825.01.T01:CDS
GGCTGAAATGATTGGAACAA+AGG 0.548087 5:-102484009 MsG0580029825.01.T01:CDS
CCAATATGGATCAAGGGGAT+GGG 0.551351 5:-102484064 None:intergenic
GTTCTAAACATTATAGGGTG+GGG 0.552349 5:-102483354 MsG0580029825.01.T01:CDS
ATATGGATCAAGGGGATGGG+TGG 0.557364 5:-102484061 MsG0580029825.01.T01:CDS
AGTTCTAAACATTATAGGGT+GGG 0.567799 5:-102483355 MsG0580029825.01.T01:CDS
TAGCATGGGGTGTAAATCCA+GGG 0.574940 5:-102484037 MsG0580029825.01.T01:CDS
TCGGTGCCAATATGGATCAA+GGG 0.576863 5:-102484070 None:intergenic
GGGGATGGGTGGATAGCATG+GGG 0.582355 5:-102484050 MsG0580029825.01.T01:CDS
TTGGAACAAAGGCAATCATA+GGG 0.588140 5:-102483998 MsG0580029825.01.T01:CDS
TGGGGAACATTGTTACCTAT+GGG 0.591485 5:-102483336 MsG0580029825.01.T01:CDS
CGGTGCCAATATGGATCAAG+GGG 0.603107 5:-102484069 None:intergenic
GAGTTCTAAACATTATAGGG+TGG 0.623171 5:-102483356 MsG0580029825.01.T01:CDS
AGTGAAAATGAGAATGCCAA+AGG 0.626910 5:+102483143 None:intergenic
TGTTACTAAGGAACCAAACA+GGG 0.643040 5:-102483934 MsG0580029825.01.T01:intron
ATGTGCAAGTAAAACCACCA+TGG 0.696565 5:+102483267 None:intergenic
AGCATGGGGTGTAAATCCAG+GGG 0.707308 5:-102484036 MsG0580029825.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCATTAGTTTTTTT+TGG - Chr5:102483714-102483733 MsG0580029825.01.T01:intron 10.0%
!! CTTATTATGTATTGAAATTT+AGG - Chr5:102483805-102483824 MsG0580029825.01.T01:intron 15.0%
!! TTATGTTATATTTATCAGAA+GGG + Chr5:102483385-102483404 None:intergenic 15.0%
!! CAATCATGTTTAATTAATTG+TGG - Chr5:102483601-102483620 MsG0580029825.01.T01:intron 20.0%
!! CTGATAAATATAACATAACA+AGG - Chr5:102483387-102483406 MsG0580029825.01.T01:intron 20.0%
!! GATTGATCTGTTAATAATAT+TGG + Chr5:102483750-102483769 None:intergenic 20.0%
!! GTTATGTTATATTTATCAGA+AGG + Chr5:102483386-102483405 None:intergenic 20.0%
!! TCTATATTGTGAATATTTGT+TGG + Chr5:102483469-102483488 None:intergenic 20.0%
! TAAAACCTATGATGTTACTA+AGG - Chr5:102483244-102483263 MsG0580029825.01.T01:CDS 25.0%
! TGTATTGAAATTTAGGTACA+TGG - Chr5:102483812-102483831 MsG0580029825.01.T01:intron 25.0%
! TGTTTAATTAATTGTGGTTC+AGG - Chr5:102483607-102483626 MsG0580029825.01.T01:intron 25.0%
!! GCTTTTATCTACAATGATAT+TGG - Chr5:102483285-102483304 MsG0580029825.01.T01:CDS 25.0%
!! TATTGTGAATATTTGTTGGA+TGG + Chr5:102483465-102483484 None:intergenic 25.0%
!!! ATGGAGTTCTAAACATTATA+GGG - Chr5:102483831-102483850 MsG0580029825.01.T01:intron 25.0%
!!! TGTTGTGAATCTTTATTTTG+CGG - Chr5:102483644-102483663 MsG0580029825.01.T01:intron 25.0%
!!! TTTTTTGTCAATAAATCACC+TGG + Chr5:102483784-102483803 None:intergenic 25.0%
ACATAGTAGCAAAAGAGAAA+AGG + Chr5:102483573-102483592 None:intergenic 30.0%
ATCATAGGGATCAAATATCA+TGG - Chr5:102483206-102483225 MsG0580029825.01.T01:CDS 30.0%
CAATGAGAAAACCAATTGTT+TGG + Chr5:102483955-102483974 None:intergenic 30.0%
!! AGTTCTAAACATTATAGGGT+GGG - Chr5:102483835-102483854 MsG0580029825.01.T01:intron 30.0%
!! ATCCTTTTAAAAAGGATCCA+TGG - Chr5:102483906-102483925 MsG0580029825.01.T01:intron 30.0%
!! CATGGAGTTCTAAACATTAT+AGG - Chr5:102483830-102483849 MsG0580029825.01.T01:intron 30.0%
!! TCGAGTGTATCCTTTTAAAA+AGG - Chr5:102483898-102483917 MsG0580029825.01.T01:intron 30.0%
!!! CAAACAATTGGTTTTCTCAT+TGG - Chr5:102483953-102483972 MsG0580029825.01.T01:CDS 30.0%
AGTGAAAATGAGAATGCCAA+AGG + Chr5:102484050-102484069 None:intergenic 35.0%
ATATGCTCGATTCAAGATGA+AGG - Chr5:102483324-102483343 MsG0580029825.01.T01:CDS 35.0%
ATGTTACTAAGGAACCAAAC+AGG - Chr5:102483255-102483274 MsG0580029825.01.T01:CDS 35.0%
ATTGGAACAAAGGCAATCAT+AGG - Chr5:102483191-102483210 MsG0580029825.01.T01:CDS 35.0%
CTTGGAATTATTACTCCCAT+AGG + Chr5:102483872-102483891 None:intergenic 35.0%
TAACAAGGTTGAACCATGTT+TGG - Chr5:102483402-102483421 MsG0580029825.01.T01:intron 35.0%
TATGCTCGATTCAAGATGAA+GGG - Chr5:102483325-102483344 MsG0580029825.01.T01:CDS 35.0%
TGCAGCAAAAATTGCAATTG+CGG - Chr5:102483672-102483691 MsG0580029825.01.T01:intron 35.0%
TGGTTCCTTAGTAACATCAT+AGG + Chr5:102483252-102483271 None:intergenic 35.0%
TGTTACTAAGGAACCAAACA+GGG - Chr5:102483256-102483275 MsG0580029825.01.T01:CDS 35.0%
TGTTAGGGTGATATACCTTT+GGG - Chr5:102483439-102483458 MsG0580029825.01.T01:intron 35.0%
TTGGAACAAAGGCAATCATA+GGG - Chr5:102483192-102483211 MsG0580029825.01.T01:CDS 35.0%
! CACCATGGATCCTTTTTAAA+AGG + Chr5:102483911-102483930 None:intergenic 35.0%
! GTTCTAAACATTATAGGGTG+GGG - Chr5:102483836-102483855 MsG0580029825.01.T01:intron 35.0%
!! CTTTTAAAAAGGATCCATGG+TGG - Chr5:102483909-102483928 MsG0580029825.01.T01:intron 35.0%
!! GAGTTCTAAACATTATAGGG+TGG - Chr5:102483834-102483853 MsG0580029825.01.T01:intron 35.0%
AAAGGATTGTTACCGATCGA+AGG + Chr5:102483555-102483574 None:intergenic 40.0%
ATCAATCTTGCATTGCATCC+AGG - Chr5:102483763-102483782 MsG0580029825.01.T01:intron 40.0%
ATGTGCAAGTAAAACCACCA+TGG + Chr5:102483926-102483945 None:intergenic 40.0%
CTGTTAGGGTGATATACCTT+TGG - Chr5:102483438-102483457 MsG0580029825.01.T01:intron 40.0%
GAAACTGAGTTTCCTTCGAT+CGG - Chr5:102483540-102483559 MsG0580029825.01.T01:intron 40.0%
GGCTGAAATGATTGGAACAA+AGG - Chr5:102483181-102483200 MsG0580029825.01.T01:CDS 40.0%
TGAACCATGTTTGGCAAGTT+GGG - Chr5:102483411-102483430 MsG0580029825.01.T01:intron 40.0%
TGGGGAACATTGTTACCTAT+GGG - Chr5:102483854-102483873 MsG0580029825.01.T01:intron 40.0%
TTCCAAACTCATCGAACCTT+TGG - Chr5:102484031-102484050 MsG0580029825.01.T01:CDS 40.0%
TTGAACCATGTTTGGCAAGT+TGG - Chr5:102483410-102483429 MsG0580029825.01.T01:intron 40.0%
! ATATTTGTTGGATGGCCCAA+AGG + Chr5:102483457-102483476 None:intergenic 40.0%
! CACATTGGTTGCCAAACAAT+TGG - Chr5:102483941-102483960 MsG0580029825.01.T01:CDS 40.0%
!! GGATACACTCGAAAGTATCT+TGG + Chr5:102483890-102483909 None:intergenic 40.0%
!! TGGTGGTTTTACTTGCACAT+TGG - Chr5:102483926-102483945 MsG0580029825.01.T01:intron 40.0%
ACTAAGGAACCAAACAGGGT+TGG - Chr5:102483260-102483279 MsG0580029825.01.T01:CDS 45.0%
ATAGACTTGACTTCTCCCGA+TGG - Chr5:102483502-102483521 MsG0580029825.01.T01:intron 45.0%
ATAGCATGGGGTGTAAATCC+AGG - Chr5:102483152-102483171 MsG0580029825.01.T01:CDS 45.0%
GTGGGGAACATTGTTACCTA+TGG - Chr5:102483853-102483872 MsG0580029825.01.T01:intron 45.0%
TAAAAGCCTCCAACCCTGTT+TGG + Chr5:102483272-102483291 None:intergenic 45.0%
TAGCATGGGGTGTAAATCCA+GGG - Chr5:102483153-102483172 MsG0580029825.01.T01:CDS 45.0%
TGCCAAAGGTTCGATGAGTT+TGG + Chr5:102484036-102484055 None:intergenic 45.0%
TTCTCATTGGTACTGCTGGT+TGG - Chr5:102483966-102483985 MsG0580029825.01.T01:CDS 45.0%
! GGTTTTCTCATTGGTACTGC+TGG - Chr5:102483962-102483981 MsG0580029825.01.T01:CDS 45.0%
! TCTCATTGGTACTGCTGGTT+GGG - Chr5:102483967-102483986 MsG0580029825.01.T01:CDS 45.0%
!! TGGGCTATTGGATTGGTTCT+TGG - Chr5:102483986-102484005 MsG0580029825.01.T01:CDS 45.0%
AAGGAACCAAACAGGGTTGG+AGG - Chr5:102483263-102483282 MsG0580029825.01.T01:CDS 50.0%
AGCATGGGGTGTAAATCCAG+GGG - Chr5:102483154-102483173 MsG0580029825.01.T01:CDS 50.0%
ATCATTTCAGCCCTTTCCCC+TGG + Chr5:102483173-102483192 None:intergenic 50.0%
CCTTCCCAACTTGCCAAACA+TGG + Chr5:102483418-102483437 None:intergenic 50.0%
GGGGAAAGGGCTGAAATGAT+TGG - Chr5:102483173-102483192 MsG0580029825.01.T01:CDS 50.0%
GGGTGTAAATCCAGGGGAAA+GGG - Chr5:102483160-102483179 MsG0580029825.01.T01:CDS 50.0%
! CCATGTTTGGCAAGTTGGGA+AGG - Chr5:102483415-102483434 MsG0580029825.01.T01:intron 50.0%
! GCAAGTTGGGAAGGCTGTTA+GGG - Chr5:102483424-102483443 MsG0580029825.01.T01:intron 50.0%
! TGCTGGTTGGGCTATTGGAT+TGG - Chr5:102483979-102483998 MsG0580029825.01.T01:CDS 50.0%
AAGGGGATGGGTGGATAGCA+TGG - Chr5:102483138-102483157 MsG0580029825.01.T01:CDS 55.0%
AGGGGATGGGTGGATAGCAT+GGG - Chr5:102483139-102483158 MsG0580029825.01.T01:CDS 55.0%
GGGGTGTAAATCCAGGGGAA+AGG - Chr5:102483159-102483178 MsG0580029825.01.T01:CDS 55.0%
! GGCAAGTTGGGAAGGCTGTT+AGG - Chr5:102483423-102483442 MsG0580029825.01.T01:intron 55.0%
! GGTACTGCTGGTTGGGCTAT+TGG - Chr5:102483974-102483993 MsG0580029825.01.T01:CDS 55.0%
CCACCCATCCCCTTGATCCA+TGG + Chr5:102483132-102483151 None:intergenic 60.0%
CTGTCCCTTGCTGCGACCAT+CGG + Chr5:102483521-102483540 None:intergenic 60.0%
GGGGATGGGTGGATAGCATG+GGG - Chr5:102483140-102483159 MsG0580029825.01.T01:CDS 60.0%
TCTCCCGATGGTCGCAGCAA+GGG - Chr5:102483514-102483533 MsG0580029825.01.T01:intron 60.0%
TGTCCCTTGCTGCGACCATC+GGG + Chr5:102483520-102483539 None:intergenic 60.0%
TTCTCCCGATGGTCGCAGCA+AGG - Chr5:102483513-102483532 MsG0580029825.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 102483131 102484081 102483131 ID=MsG0580029825.01;Name=MsG0580029825.01
Chr5 mRNA 102483131 102484081 102483131 ID=MsG0580029825.01.T01;Parent=MsG0580029825.01;Name=MsG0580029825.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|0|0|2|0|134
Chr5 exon 102483935 102484081 102483935 ID=MsG0580029825.01.T01:exon:8798;Parent=MsG0580029825.01.T01
Chr5 exon 102483131 102483385 102483131 ID=MsG0580029825.01.T01:exon:8797;Parent=MsG0580029825.01.T01
Chr5 CDS 102483935 102484081 102483935 ID=MsG0580029825.01.T01:cds;Parent=MsG0580029825.01.T01
Chr5 CDS 102483131 102483385 102483131 ID=MsG0580029825.01.T01:cds;Parent=MsG0580029825.01.T01
Gene Sequence

>MsG0580029825.01.T01

ATGGATCAAGGGGATGGGTGGATAGCATGGGGTGTAAATCCAGGGGAAAGGGCTGAAATGATTGGAACAAAGGCAATCATAGGGATCAAATATCATGGTACATTTTTGCAAGTTAAAACCTATGATGTTACTAAGGAACCAAACAGGGTACATGGAGTTCTAAACATTATAGGGTGGGGAACATTGTTACCTATGGGAGTAATAATTCCAAGATACTTTCGAGTGTATCCTTTTAAAAAGGATCCATGGTGGTTTTACTTGCACATTGGTTGCCAAACAATTGGTTTTCTCATTGGTACTGCTGGTTGGGCTATTGGATTGGTTCTTGGACATTCTTCTAGATACTATGTCTTCCAAACTCATCGAACCTTTGGCATTCTCATTTTCACTTTAGTACAATCC

Protein sequence

>MsG0580029825.01.T01

MDQGDGWIAWGVNPGERAEMIGTKAIIGIKYHGTFLQVKTYDVTKEPNRVHGVLNIIGWGTLLPMGVIIPRYFRVYPFKKDPWWFYLHIGCQTIGFLIGTAGWAIGLVLGHSSRYYVFQTHRTFGILIFTLVQS