AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010779.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010779.01.T01 MTR_2g090660 88.618 246 23 3 1 244 1 243 2.68e-128 363
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010779.01.T01 AT1G03820 32.157 255 129 10 1 244 1 222 5.39e-14 69.3

Find 34 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAGTGACACAAGATTTC+TGG 0.224853 2:-74966474 MsG0280010779.01.T01:CDS
GAGAAGTATAACAACGTTAA+TGG 0.257730 2:-74966413 MsG0280010779.01.T01:CDS
TTTCCTTGGTTGGAGAATAT+TGG 0.318029 2:+74966750 None:intergenic
AACGTTAATGGATATGAATC+AGG 0.326674 2:-74966401 MsG0280010779.01.T01:CDS
TCAAAATGAGTTTGAGACTA+TGG 0.340281 2:-74966333 MsG0280010779.01.T01:CDS
GCAGCTGCAGGTTCTTGTGC+TGG 0.400119 2:+74966875 None:intergenic
GTCGAAACAGGAAACGGTTA+CGG 0.431424 2:-74966794 MsG0280010779.01.T01:CDS
GGAAACGGTTACGGTCTCTA+TGG 0.434656 2:-74966785 MsG0280010779.01.T01:CDS
ACAATCTCTGGTGCAGCTGC+AGG 0.454852 2:+74966863 None:intergenic
TCTTGTGCTGGTGCTGATAC+CGG 0.463050 2:+74966887 None:intergenic
TTGAGGGTCTTCAACATTGT+GGG 0.464139 2:+74966940 None:intergenic
GACACAAGATTTCTGGAGAA+TGG 0.469127 2:-74966467 MsG0280010779.01.T01:CDS
GTATCAACTACTCTACCTTC+AGG 0.472495 2:-74966842 MsG0280010779.01.T01:CDS
GCTGGTGCTGATACCGGTGC+TGG 0.477279 2:+74966893 None:intergenic
GCTGATACCGGTGCTGGTGC+CGG 0.484693 2:+74966899 None:intergenic
AGAGTAGTTGATACAATCTC+TGG 0.485770 2:+74966851 None:intergenic
AACAATTACAACAGCTACAA+TGG 0.499851 2:-74966548 MsG0280010779.01.T01:CDS
TCCAGCACCGGCACCAGCAC+CGG 0.523732 2:-74966906 MsG0280010779.01.T01:intron
GGTTCTGGTGCTGGTCCTGA+AGG 0.535734 2:+74966827 None:intergenic
TTGTTGCGGAAGTTTGTTTG+AGG 0.543740 2:+74966614 None:intergenic
ATTGAGGGTCTTCAACATTG+TGG 0.549263 2:+74966939 None:intergenic
AGGGTCTTCAACATTGTGGG+TGG 0.554411 2:+74966943 None:intergenic
TAGCTTGAATTTGGGGTGAG+AGG 0.561749 2:+74967005 None:intergenic
TAGCCAATATTCTCCAACCA+AGG 0.580385 2:-74966753 MsG0280010779.01.T01:CDS
AACAACAATTATGAGAGGAA+AGG 0.589017 2:-74966503 MsG0280010779.01.T01:CDS
AATTATGAGAGGAAAGGAGA+AGG 0.592187 2:-74966497 MsG0280010779.01.T01:CDS
AATGGATATGAATCAGGGAG+AGG 0.596788 2:-74966395 MsG0280010779.01.T01:CDS
AGAGGAAGCACTGAAAATGA+AGG 0.608381 2:-74966377 MsG0280010779.01.T01:CDS
TGTACACTTCATTGTTGTTG+CGG 0.612560 2:+74966600 None:intergenic
TTACAACAGCTACAATGGAA+AGG 0.631436 2:-74966543 MsG0280010779.01.T01:CDS
ACGTTAATGGATATGAATCA+GGG 0.631473 2:-74966400 MsG0280010779.01.T01:CDS
ACAATAACAACAATTATGAG+AGG 0.640620 2:-74966508 MsG0280010779.01.T01:CDS
GAGAAGCAGCAAGAAGCACA+AGG 0.650203 2:-74966302 MsG0280010779.01.T01:CDS
GGTTACACATACACACCATG+AGG 0.736316 2:-74966281 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATAAAAGCTACAAAAA+AGG - Chr2:74966697-74966716 MsG0280010779.01.T01:intron 20.0%
!!! AAATCTGTTAATGTTGTTTT+TGG + Chr2:74966616-74966635 None:intergenic 20.0%
! ACAATAACAACAATTATGAG+AGG - Chr2:74966830-74966849 MsG0280010779.01.T01:CDS 25.0%
AACAACAATTATGAGAGGAA+AGG - Chr2:74966835-74966854 MsG0280010779.01.T01:CDS 30.0%
AACAATTACAACAGCTACAA+TGG - Chr2:74966790-74966809 MsG0280010779.01.T01:CDS 30.0%
ACAAGAAAAAGAAAGAGACT+TGG + Chr2:74966303-74966322 None:intergenic 30.0%
! AACGTTAATGGATATGAATC+AGG - Chr2:74966937-74966956 MsG0280010779.01.T01:intron 30.0%
! ACGTTAATGGATATGAATCA+GGG - Chr2:74966938-74966957 MsG0280010779.01.T01:intron 30.0%
! AGTCTCTTTCTTTTTCTTGT+TGG - Chr2:74966303-74966322 MsG0280010779.01.T01:CDS 30.0%
! GAGAAGTATAACAACGTTAA+TGG - Chr2:74966925-74966944 MsG0280010779.01.T01:intron 30.0%
! TCAAAATGAGTTTGAGACTA+TGG - Chr2:74967005-74967024 MsG0280010779.01.T01:CDS 30.0%
!! GTTTTGTCTAGCTTGAATTT+GGG + Chr2:74966344-74966363 None:intergenic 30.0%
!! TGTTTTGTCTAGCTTGAATT+TGG + Chr2:74966345-74966364 None:intergenic 30.0%
!! TTTTGTCTAGCTTGAATTTG+GGG + Chr2:74966343-74966362 None:intergenic 30.0%
!! TTTTTAGTCGAAACAGGAAA+CGG - Chr2:74966538-74966557 MsG0280010779.01.T01:CDS 30.0%
AATGAGTGACACAAGATTTC+TGG - Chr2:74966864-74966883 MsG0280010779.01.T01:CDS 35.0%
AATTATGAGAGGAAAGGAGA+AGG - Chr2:74966841-74966860 MsG0280010779.01.T01:CDS 35.0%
CCTGTTTCGACTAAAAATTG+AGG + Chr2:74966535-74966554 None:intergenic 35.0%
TCGACTAAAAATTGAGGTTC+TGG + Chr2:74966529-74966548 None:intergenic 35.0%
TTACAACAGCTACAATGGAA+AGG - Chr2:74966795-74966814 MsG0280010779.01.T01:CDS 35.0%
TTTCCTTGGTTGGAGAATAT+TGG + Chr2:74966591-74966610 None:intergenic 35.0%
! AGAGTAGTTGATACAATCTC+TGG + Chr2:74966490-74966509 None:intergenic 35.0%
! TGTACACTTCATTGTTGTTG+CGG + Chr2:74966741-74966760 None:intergenic 35.0%
!!! CCTCAATTTTTAGTCGAAAC+AGG - Chr2:74966532-74966551 MsG0280010779.01.T01:CDS 35.0%
!!! TGTTGTTTTTGGAGTTTCCT+TGG + Chr2:74966605-74966624 None:intergenic 35.0%
AATGGATATGAATCAGGGAG+AGG - Chr2:74966943-74966962 MsG0280010779.01.T01:intron 40.0%
ATTGAGGGTCTTCAACATTG+TGG + Chr2:74966402-74966421 None:intergenic 40.0%
CTGGAGATTGAGGTAATTGA+GGG + Chr2:74966417-74966436 None:intergenic 40.0%
GTATCAACTACTCTACCTTC+AGG - Chr2:74966496-74966515 MsG0280010779.01.T01:CDS 40.0%
TAGCCAATATTCTCCAACCA+AGG - Chr2:74966585-74966604 MsG0280010779.01.T01:CDS 40.0%
TTGAGGGTCTTCAACATTGT+GGG + Chr2:74966401-74966420 None:intergenic 40.0%
! AAAAATTGAGGTTCTGGTGC+TGG + Chr2:74966523-74966542 None:intergenic 40.0%
! GACACAAGATTTCTGGAGAA+TGG - Chr2:74966871-74966890 MsG0280010779.01.T01:CDS 40.0%
! TTGTTGCGGAAGTTTGTTTG+AGG + Chr2:74966727-74966746 None:intergenic 40.0%
!! AGAGGAAGCACTGAAAATGA+AGG - Chr2:74966961-74966980 MsG0280010779.01.T01:CDS 40.0%
!!! GTTTTTGGAGTTTCCTTGGT+TGG + Chr2:74966601-74966620 None:intergenic 40.0%
ATTACCTCAATCTCCAGCAC+CGG - Chr2:74966420-74966439 MsG0280010779.01.T01:CDS 45.0%
GCTGGAGATTGAGGTAATTG+AGG + Chr2:74966418-74966437 None:intergenic 45.0%
GTCGAAACAGGAAACGGTTA+CGG - Chr2:74966544-74966563 MsG0280010779.01.T01:CDS 45.0%
! TAGCTTGAATTTGGGGTGAG+AGG + Chr2:74966336-74966355 None:intergenic 45.0%
AGGGTCTTCAACATTGTGGG+TGG + Chr2:74966398-74966417 None:intergenic 50.0%
GGAAACGGTTACGGTCTCTA+TGG - Chr2:74966553-74966572 MsG0280010779.01.T01:CDS 50.0%
! GAGAAGCAGCAAGAAGCACA+AGG - Chr2:74967036-74967055 MsG0280010779.01.T01:CDS 50.0%
!! TCTTGTGCTGGTGCTGATAC+CGG + Chr2:74966454-74966473 None:intergenic 50.0%
ACAATCTCTGGTGCAGCTGC+AGG + Chr2:74966478-74966497 None:intergenic 55.0%
GCAGCTGCAGGTTCTTGTGC+TGG + Chr2:74966466-74966485 None:intergenic 60.0%
!! GGTTCTGGTGCTGGTCCTGA+AGG + Chr2:74966514-74966533 None:intergenic 60.0%
!! GCTGATACCGGTGCTGGTGC+CGG + Chr2:74966442-74966461 None:intergenic 65.0%
!! GCTGGTGCTGATACCGGTGC+TGG + Chr2:74966448-74966467 None:intergenic 65.0%
!! GGTGCCGGTGCTGGAGATTG+AGG + Chr2:74966427-74966446 None:intergenic 65.0%
!! TCCAGCACCGGCACCAGCAC+CGG - Chr2:74966432-74966451 MsG0280010779.01.T01:CDS 70.0%
!! ACCGGTGCTGGTGCCGGTGC+TGG + Chr2:74966436-74966455 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 74966283 74967077 74966283 ID=MsG0280010779.01;Name=MsG0280010779.01
Chr2 mRNA 74966283 74967077 74966283 ID=MsG0280010779.01.T01;Parent=MsG0280010779.01;Name=MsG0280010779.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|3|0|244
Chr2 exon 74966283 74966671 74966283 ID=MsG0280010779.01.T01:exon:11098;Parent=MsG0280010779.01.T01
Chr2 exon 74966702 74966923 74966702 ID=MsG0280010779.01.T01:exon:11097;Parent=MsG0280010779.01.T01
Chr2 exon 74966954 74967077 74966954 ID=MsG0280010779.01.T01:exon:11096;Parent=MsG0280010779.01.T01
Chr2 CDS 74966954 74967077 74966954 ID=MsG0280010779.01.T01:cds;Parent=MsG0280010779.01.T01
Chr2 CDS 74966702 74966923 74966702 ID=MsG0280010779.01.T01:cds;Parent=MsG0280010779.01.T01
Chr2 CDS 74966283 74966671 74966283 ID=MsG0280010779.01.T01:cds;Parent=MsG0280010779.01.T01
Gene Sequence

>MsG0280010779.01.T01

ATGGCTTCTCTTGCAAACCAAGTCTCTTTCTTTTTCTTGTTGGTACTTTTCCTCTCACCCCAAATTCAAGCTAGACAAAACAAGTTCTTTAGCTTCTTTACTCAATTCAAAACCACCCACAATGCACCGGCACCAGCACCGGTATCAGCACCAGCACAAGAACCTGCAGCTGCACCAGAGATTGTATCAACTACTCTACCTTCAGGACCAGCACCAGAACCTCAATTTTTAGTCGAAACAGGAAACGGTTACGGTCTCTATGGCATTGATTCTAGCCAATATTCTCCAACCAAGGAAACTCCAAAAACAACATTAACAGATTTTGAAGATGAGCTTCTCAATGAAGATTTCAATGATAATAAAAGCTACAAAAAAGGATATCCTCAAACAAACTTCCGCAACAACAATGAAGTGTACACAAAAAACTACAACAATGAAGAGAACAAGAACAATTACAACAGCTACAATGGAAAGGAGTTTTACAACAACAATAACAACAATTATGAGAGGAAAGGAGAAGGAATGAGTGACACAAGATTTCTGGAGAATGGAAAGTACTATTACAATGTAAATAGTGAAAATGAGAAGTATAACAACGTTAATGGATATGAATCAGGGAGAGGAAGCACTGAAAATGAAGGTAACTATGAAAAAAATCAGTATCAAAATGAGTTTGAGACTATGGAAGAGTATGAGAAGCAGCAAGAAGCACAAGGTTACACATACACACCATGA

Protein sequence

>MsG0280010779.01.T01

MASLANQVSFFFLLVLFLSPQIQARQNKFFSFFTQFKTTHNAPAPAPVSAPAQEPAAAPEIVSTTLPSGPAPEPQFLVETGNGYGLYGIDSSQYSPTKETPKTTLTDFEDELLNEDFNDNKSYKKGYPQTNFRNNNEVYTKNYNNEENKNNYNSYNGKEFYNNNNNNYERKGEGMSDTRFLENGKYYYNVNSENEKYNNVNGYESGRGSTENEGNYEKNQYQNEFETMEEYEKQQEAQGYTYTP*