AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004257.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004257.01.T02 MTR_1g076130 99.699 332 1 0 1 332 1 332 0.0 681
MsG0180004257.01.T02 MTR_1g043040 93.675 332 21 0 1 332 1 332 0.0 623
MsG0180004257.01.T02 MTR_8g005980 84.498 329 51 0 3 331 85 413 0.0 559
MsG0180004257.01.T02 MTR_1g090730 39.818 329 191 4 3 328 92 416 6.11e-74 234
MsG0180004257.01.T02 MTR_1g090730 41.852 270 153 1 3 272 92 357 1.91e-62 204
MsG0180004257.01.T02 MTR_7g037650 73.913 46 12 0 15 60 24 69 7.09e-14 72.8
MsG0180004257.01.T01 MTR_1g076130 99.699 332 1 0 1 332 1 332 0.0 681
MsG0180004257.01.T01 MTR_1g043040 93.675 332 21 0 1 332 1 332 0.0 623
MsG0180004257.01.T01 MTR_8g005980 84.498 329 51 0 3 331 85 413 0.0 559
MsG0180004257.01.T01 MTR_1g090730 39.818 329 191 4 3 328 92 416 6.11e-74 234
MsG0180004257.01.T01 MTR_1g090730 41.852 270 153 1 3 272 92 357 1.91e-62 204
MsG0180004257.01.T01 MTR_7g037650 73.913 46 12 0 15 60 24 69 7.09e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004257.01.T02 AT1G04410 90.060 332 33 0 1 332 1 332 0.0 605
MsG0180004257.01.T02 AT5G43330 89.458 332 35 0 1 332 1 332 0.0 597
MsG0180004257.01.T02 AT5G56720 72.590 332 91 0 1 332 40 371 0.0 510
MsG0180004257.01.T02 AT5G58330 41.641 329 185 4 3 328 96 420 5.38e-79 248
MsG0180004257.01.T02 AT5G58330 41.641 329 185 4 3 328 97 421 6.03e-79 248
MsG0180004257.01.T02 AT5G58330 41.587 315 177 4 17 328 2 312 5.18e-76 237
MsG0180004257.01.T01 AT1G04410 90.060 332 33 0 1 332 1 332 0.0 605
MsG0180004257.01.T01 AT5G43330 89.458 332 35 0 1 332 1 332 0.0 597
MsG0180004257.01.T01 AT5G56720 72.590 332 91 0 1 332 40 371 0.0 510
MsG0180004257.01.T01 AT5G58330 41.641 329 185 4 3 328 96 420 5.38e-79 248
MsG0180004257.01.T01 AT5G58330 41.641 329 185 4 3 328 97 421 6.03e-79 248
MsG0180004257.01.T01 AT5G58330 41.587 315 177 4 17 328 2 312 5.18e-76 237

Find 13 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTGAAACGACACCGCTTT+CGG 0.431818 1:-75645992 None:intergenic
CCTGTTCGTGTTCTTGTCAC+TGG 0.433814 1:+75646172 MsG0180004257.01.T01:CDS
CCAGTGACAAGAACACGAAC+AGG 0.438629 1:-75646172 None:intergenic
ACTATTTGTCTATTTATTCA+CGG 0.445259 1:-75646045 None:intergenic
AGAGAATATTCTATCTTGCT+TGG 0.464290 1:+75645911 MsG0180004257.01.T02:five_prime_UTR
GTTCTTGTCACTGGTGCTGC+AGG 0.494198 1:+75646181 MsG0180004257.01.T01:CDS
TAAAGGAGATTGCTGATAAG+TGG 0.498193 1:-75645941 None:intergenic
TTCATGAGTAGGAGTAGCAT+TGG 0.507726 1:+75646017 MsG0180004257.01.T01:five_prime_UTR
GTTTCACGCCTTTCATGAGT+AGG 0.532258 1:+75646006 MsG0180004257.01.T01:five_prime_UTR
ATATTCTATCTTGCTTGGCA+TGG 0.544371 1:+75645916 MsG0180004257.01.T02:five_prime_UTR
ATGCTACTCCTACTCATGAA+AGG 0.602446 1:-75646014 None:intergenic
AATAATAGAGGAACCGAAAG+CGG 0.626618 1:+75645979 MsG0180004257.01.T01:five_prime_UTR
CAGTGACAAGAACACGAACA+GGG 0.694789 1:-75646171 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTATTATTATTTAA+AGG - Chr1:75645961-75645980 None:intergenic 0.0%
!! AACATTTATCAATAAAATAG+TGG - Chr1:75649443-75649462 None:intergenic 15.0%
!! AGAAAATTGCATAATTATAA+GGG - Chr1:75646511-75646530 None:intergenic 15.0%
!! ATGTGAAAAATGTTATAATT+TGG + Chr1:75648166-75648185 MsG0180004257.01.T01:CDS 15.0%
!! CTTAATTTACAAATAAAAGA+CGG - Chr1:75648829-75648848 None:intergenic 15.0%
!! TGTGAAAAATGTTATAATTT+GGG + Chr1:75648167-75648186 MsG0180004257.01.T01:CDS 15.0%
!! TTCAAATTCTTTATATTGAA+TGG + Chr1:75648569-75648588 MsG0180004257.01.T01:intron 15.0%
!!! ACTCTTTGTTTTTTTTTTTT+TGG + Chr1:75649290-75649309 MsG0180004257.01.T01:three_prime_UTR 15.0%
!!! ATTTTTTTTGACATAAAATG+CGG - Chr1:75647392-75647411 None:intergenic 15.0%
!!! TTGTTTTATATAGCAAATAA+AGG + Chr1:75648855-75648874 MsG0180004257.01.T01:intron 15.0%
!! AAAAAATATGTTCCATTCTA+TGG + Chr1:75647405-75647424 MsG0180004257.01.T01:intron 20.0%
!! ACAACTTAATTAAATTCGTA+TGG - Chr1:75647694-75647713 None:intergenic 20.0%
!! ACTATTTGTCTATTTATTCA+CGG - Chr1:75646048-75646067 None:intergenic 20.0%
!! ATTTCAACATAAAATCTTTG+AGG + Chr1:75646796-75646815 MsG0180004257.01.T01:intron 20.0%
!! ATTTCTATAAACTCTCAAAT+AGG - Chr1:75646562-75646581 None:intergenic 20.0%
!! CAGAAAATTGCATAATTATA+AGG - Chr1:75646512-75646531 None:intergenic 20.0%
!! GTGAAAAATGTTATAATTTG+GGG + Chr1:75648168-75648187 MsG0180004257.01.T01:CDS 20.0%
!! GTTTCCAAAAATATGTAAAA+AGG - Chr1:75647935-75647954 None:intergenic 20.0%
!! TAGTGGTAAAATATCAAATA+CGG - Chr1:75649426-75649445 None:intergenic 20.0%
!! TTGTGAAAATTATTTGTTTC+AGG + Chr1:75647130-75647149 MsG0180004257.01.T01:intron 20.0%
!! TTTAGAAATAGAACTATGTT+TGG + Chr1:75646339-75646358 MsG0180004257.01.T01:intron 20.0%
!! TTTCTATAAACTCTCAAATA+GGG - Chr1:75646561-75646580 None:intergenic 20.0%
!!! AAAAATGTTTTGCCAATTTT+AGG + Chr1:75646838-75646857 MsG0180004257.01.T01:intron 20.0%
!!! AGATTTTGATAAATTATTGC+TGG + Chr1:75648752-75648771 MsG0180004257.01.T01:intron 20.0%
!!! CGTCTTTTATTTGTAAATTA+AGG + Chr1:75648827-75648846 MsG0180004257.01.T01:intron 20.0%
!!! CTTTTGTTTTGTTTTGTTTA+AGG + Chr1:75646242-75646261 MsG0180004257.01.T01:intron 20.0%
!!! GTAAACATTTTAACCATATT+TGG - Chr1:75646762-75646781 None:intergenic 20.0%
!!! TACGAATTTAATTAAGTTGT+TGG + Chr1:75647694-75647713 MsG0180004257.01.T01:intron 20.0%
!!! TAGTCCTTTTTACATATTTT+TGG + Chr1:75647928-75647947 MsG0180004257.01.T01:intron 20.0%
!!! TCAATTCTCAATTTTGAATA+AGG + Chr1:75649072-75649091 MsG0180004257.01.T01:three_prime_UTR 20.0%
! AAAAAATTCTAGTAGTTGCA+AGG + Chr1:75646427-75646446 MsG0180004257.01.T01:intron 25.0%
! AAATGCTTTGCAAGATTAAA+AGG + Chr1:75647105-75647124 MsG0180004257.01.T01:intron 25.0%
! AACTAAAATAAATTCAGTGC+AGG - Chr1:75647793-75647812 None:intergenic 25.0%
! AATTGAGAATTGACAAACAA+TGG - Chr1:75649065-75649084 None:intergenic 25.0%
! ACAGAGTTTAAAGAAATGTA+AGG - Chr1:75647980-75647999 None:intergenic 25.0%
! AGGTAACCATAATAAATTCT+GGG + Chr1:75646201-75646220 MsG0180004257.01.T01:intron 25.0%
! ATACTTCAGTAAATGCAAAA+TGG - Chr1:75648364-75648383 None:intergenic 25.0%
! TATCCAATTTGTCCTAAAAT+TGG - Chr1:75646853-75646872 None:intergenic 25.0%
! TCTTTCATCCAAAAAAACAA+TGG + Chr1:75646141-75646160 MsG0180004257.01.T01:exon 25.0%
! TGAAAGAGATACATGAAAAA+GGG - Chr1:75647441-75647460 None:intergenic 25.0%
! TGAAGTTGTTTATCCAAATA+TGG + Chr1:75646746-75646765 MsG0180004257.01.T01:intron 25.0%
! TTTATTTCCATAATCTCTCT+AGG + Chr1:75646613-75646632 MsG0180004257.01.T01:intron 25.0%
! TTTGAAAAGTTTATGTCTTC+TGG + Chr1:75649235-75649254 MsG0180004257.01.T01:three_prime_UTR 25.0%
!! AAACAAGAAATGTTTTTCTC+AGG - Chr1:75648081-75648100 None:intergenic 25.0%
!! TAAATGTTACTTGAACTTCT+TGG + Chr1:75647039-75647058 MsG0180004257.01.T01:intron 25.0%
!! TATCAATTGATGAGTTTTCA+AGG + Chr1:75648934-75648953 MsG0180004257.01.T01:CDS 25.0%
!! TTTTCATTGCAATATCATCA+GGG + Chr1:75648616-75648635 MsG0180004257.01.T01:intron 25.0%
!!! ATCTGTCTAATTTTTTAACC+AGG + Chr1:75648909-75648928 MsG0180004257.01.T01:intron 25.0%
!!! ATGTGTATTTTGGTCATATA+TGG + Chr1:75647847-75647866 MsG0180004257.01.T01:intron 25.0%
!!! TGAGTTTTCAAGGAAAAAAT+TGG + Chr1:75648944-75648963 MsG0180004257.01.T01:CDS 25.0%
!!! TGTTTTTTTGGATGAAAGAA+AGG - Chr1:75646140-75646159 None:intergenic 25.0%
AAAGAGATACATGAAAAAGG+GGG - Chr1:75647439-75647458 None:intergenic 30.0%
AAGTGAATAAGTGGGAAATT+CGG + Chr1:75649347-75649366 MsG0180004257.01.T01:three_prime_UTR 30.0%
AAGTTATCCTAGAGAGATTA+TGG - Chr1:75646623-75646642 None:intergenic 30.0%
ACTCCTTTAAGTGAATAAGT+GGG + Chr1:75649339-75649358 MsG0180004257.01.T01:three_prime_UTR 30.0%
AGAGAATATTCTATCTTGCT+TGG + Chr1:75645911-75645930 MsG0180004257.01.T02:five_prime_UTR 30.0%
AGATATTGAGAAACTCTTGA+TGG + Chr1:75649103-75649122 MsG0180004257.01.T01:three_prime_UTR 30.0%
ATATTGAATGGCTTCAACTA+AGG + Chr1:75648581-75648600 MsG0180004257.01.T01:intron 30.0%
ATCATCAACTCATACTATAC+TGG - Chr1:75649492-75649511 None:intergenic 30.0%
CAATCAAGAAACATGATACA+AGG - Chr1:75646313-75646332 None:intergenic 30.0%
CAGGTAACCATAATAAATTC+TGG + Chr1:75646200-75646219 MsG0180004257.01.T01:intron 30.0%
CTGAAAGAGATACATGAAAA+AGG - Chr1:75647442-75647461 None:intergenic 30.0%
CTTGTATCATGTTTCTTGAT+TGG + Chr1:75646311-75646330 MsG0180004257.01.T01:intron 30.0%
GAAAGAGATACATGAAAAAG+GGG - Chr1:75647440-75647459 None:intergenic 30.0%
GAGAAACCCAGAATTTATTA+TGG - Chr1:75646210-75646229 None:intergenic 30.0%
TAAGGACATTACAATATGAG+AGG - Chr1:75649387-75649406 None:intergenic 30.0%
TAGGGAAAATACTACAATTG+AGG - Chr1:75646543-75646562 None:intergenic 30.0%
TCAAATAAGTCAATCCAAAC+AGG - Chr1:75647466-75647485 None:intergenic 30.0%
TTTCCCACTTATTCACTTAA+AGG - Chr1:75649345-75649364 None:intergenic 30.0%
TTTCCTTGTGTATTTGTCAA+TGG + Chr1:75649034-75649053 MsG0180004257.01.T01:three_prime_UTR 30.0%
! AAACTCAAACCTTTAAGAAG+AGG - Chr1:75647009-75647028 None:intergenic 30.0%
! AACTCAAACCTTTAAGAAGA+GGG - Chr1:75647008-75647027 None:intergenic 30.0%
! AGAAATGTTTTTCTCAGGAA+TGG - Chr1:75648076-75648095 None:intergenic 30.0%
! AGACATAAACTTTTCAAACG+CGG - Chr1:75649233-75649252 None:intergenic 30.0%
! CAAGTGATGATTTGCATAAA+AGG - Chr1:75649138-75649157 None:intergenic 30.0%
! CATTTCTTGTTTGACAAGAT+TGG + Chr1:75648089-75648108 MsG0180004257.01.T01:CDS 30.0%
! CTTTTCATTGCAATATCATC+AGG + Chr1:75648615-75648634 MsG0180004257.01.T01:intron 30.0%
! TGATGTAATTTTCATCGTCA+AGG + Chr1:75648384-75648403 MsG0180004257.01.T01:intron 30.0%
! TTAGTTTAGTGCTGAAAAAG+TGG + Chr1:75647807-75647826 MsG0180004257.01.T01:intron 30.0%
! TTGTCATAGTTTTTGTGTGA+TGG + Chr1:75647742-75647761 MsG0180004257.01.T01:intron 30.0%
! TTTTCATCGTCAAGGTTAAA+TGG + Chr1:75648392-75648411 MsG0180004257.01.T01:intron 30.0%
!!! GTCTTTAGCCATTGTTTTTT+TGG - Chr1:75646152-75646171 None:intergenic 30.0%
!!! TTGCCAATTTTAGGACAAAT+TGG + Chr1:75646847-75646866 MsG0180004257.01.T01:intron 30.0%
AATAATAGAGGAACCGAAAG+CGG + Chr1:75645979-75645998 MsG0180004257.01.T01:five_prime_UTR 35.0%
AATACGGAGCATAACTTAGT+TGG - Chr1:75649410-75649429 None:intergenic 35.0%
AGAAACAGATTTCACCAGAA+TGG - Chr1:75647889-75647908 None:intergenic 35.0%
ATATTCTATCTTGCTTGGCA+TGG + Chr1:75645916-75645935 MsG0180004257.01.T02:five_prime_UTR 35.0%
ATTCAGTGCAGGTAATGTAT+TGG - Chr1:75647782-75647801 None:intergenic 35.0%
ATTCCCTAGAAAAGAAGGTA+TGG + Chr1:75647219-75647238 MsG0180004257.01.T01:CDS 35.0%
ATTGAGAAACTCTTGATGGA+TGG + Chr1:75649107-75649126 MsG0180004257.01.T01:three_prime_UTR 35.0%
CACTCCTTTAAGTGAATAAG+TGG + Chr1:75649338-75649357 MsG0180004257.01.T01:three_prime_UTR 35.0%
CCAACTTCAAACCAGAAATA+TGG - Chr1:75647069-75647088 None:intergenic 35.0%
CTTATTTCCATAAGCTCTAC+AGG + Chr1:75647575-75647594 MsG0180004257.01.T01:intron 35.0%
GACATTACAATATGAGAGGA+CGG - Chr1:75649383-75649402 None:intergenic 35.0%
GCTTGAGCATTCAGAATTAA+AGG - Chr1:75647336-75647355 None:intergenic 35.0%
TAAAGGAGATTGCTGATAAG+TGG - Chr1:75645944-75645963 None:intergenic 35.0%
TATTTGTCCATCAACATACC+AGG - Chr1:75648289-75648308 None:intergenic 35.0%
TTGTGACCACATTAGAGATT+GGG + Chr1:75648502-75648521 MsG0180004257.01.T01:CDS 35.0%
TTTAACACCGTTCAGTGATT+CGG - Chr1:75646962-75646981 None:intergenic 35.0%
! AACTCATCAATTGATAGTCC+TGG - Chr1:75648930-75648949 None:intergenic 35.0%
! AATATTGCTGTTATGGTTGG+TGG + Chr1:75647196-75647215 MsG0180004257.01.T01:CDS 35.0%
! AATGAATAGATTAGTCCAGC+GGG - Chr1:75648688-75648707 None:intergenic 35.0%
! ATCACTGAACGGTGTTAAAA+TGG + Chr1:75646963-75646982 MsG0180004257.01.T01:CDS 35.0%
! ATGTTTTTCTCAGGAATGGA+TGG - Chr1:75648072-75648091 None:intergenic 35.0%
! GAATGAATAGATTAGTCCAG+CGG - Chr1:75648689-75648708 None:intergenic 35.0%
! GTCAATATTGCTGTTATGGT+TGG + Chr1:75647193-75647212 MsG0180004257.01.T01:CDS 35.0%
! TCTCCATACCTTCTTTTCTA+GGG - Chr1:75647225-75647244 None:intergenic 35.0%
! TGGAGTCAATATTGCTGTTA+TGG + Chr1:75647189-75647208 MsG0180004257.01.T01:CDS 35.0%
!! AAGCAATTTTAACAGTTGCG+TGG - Chr1:75648227-75648246 None:intergenic 35.0%
!! CCATATTTCTGGTTTGAAGT+TGG + Chr1:75647066-75647085 MsG0180004257.01.T01:intron 35.0%
!! TGGTTGGTTCATGTGTATTT+TGG + Chr1:75647837-75647856 MsG0180004257.01.T01:intron 35.0%
!! TTTTTGGCGAATTAACCTGT+TGG + Chr1:75649306-75649325 MsG0180004257.01.T01:three_prime_UTR 35.0%
AAAAAGTGGTTCCATCTGGT+TGG + Chr1:75647821-75647840 MsG0180004257.01.T01:intron 40.0%
AAGCTATCCTGTAGAGCTTA+TGG - Chr1:75647585-75647604 None:intergenic 40.0%
AAGTCCTTGTGATTCCATTC+TGG + Chr1:75647872-75647891 MsG0180004257.01.T01:intron 40.0%
AGCGAATTCCTTCAAGATCA+AGG - Chr1:75648049-75648068 None:intergenic 40.0%
ATACACATGAACCAACCAGA+TGG - Chr1:75647835-75647854 None:intergenic 40.0%
ATGCTACTCCTACTCATGAA+AGG - Chr1:75646017-75646036 None:intergenic 40.0%
CACATTAGAGATTGGGTTCT+AGG + Chr1:75648509-75648528 MsG0180004257.01.T01:CDS 40.0%
CCTCCATTGACAAATACACA+AGG - Chr1:75649040-75649059 None:intergenic 40.0%
CCTTGTGTATTTGTCAATGG+AGG + Chr1:75649037-75649056 MsG0180004257.01.T01:three_prime_UTR 40.0%
CTAGAACCCAATCTCTAATG+TGG - Chr1:75648511-75648530 None:intergenic 40.0%
CTTGTGACCACATTAGAGAT+TGG + Chr1:75648501-75648520 MsG0180004257.01.T01:CDS 40.0%
GACAAGATTGGACCATAACA+GGG + Chr1:75648101-75648120 MsG0180004257.01.T01:CDS 40.0%
GGAGTGAATTTATAGCCAAC+AGG - Chr1:75649324-75649343 None:intergenic 40.0%
GTATCTCTTTCAGACCTGTT+TGG + Chr1:75647449-75647468 MsG0180004257.01.T01:intron 40.0%
TAAACTCTGTATCACCATGC+AGG + Chr1:75647990-75648009 MsG0180004257.01.T01:intron 40.0%
TCGATGGGAGTTTATTCTGA+TGG + Chr1:75648649-75648668 MsG0180004257.01.T01:CDS 40.0%
TGACAAGATTGGACCATAAC+AGG + Chr1:75648100-75648119 MsG0180004257.01.T01:CDS 40.0%
TGTTGTTGCTACAACTGATG+TGG + Chr1:75647153-75647172 MsG0180004257.01.T01:CDS 40.0%
TTCACCAGAATGGAATCACA+AGG - Chr1:75647879-75647898 None:intergenic 40.0%
TTCATGAGTAGGAGTAGCAT+TGG + Chr1:75646017-75646036 MsG0180004257.01.T01:five_prime_UTR 40.0%
TTCCTTCAAGATCAAGGCAT+TGG - Chr1:75648043-75648062 None:intergenic 40.0%
TTCTTGGACAGCCATATTTC+TGG + Chr1:75647055-75647074 MsG0180004257.01.T01:intron 40.0%
TTTCTCAAGAGCAGAAGCTT+GGG - Chr1:75647287-75647306 None:intergenic 40.0%
! CTAGAAAAGAAGGTATGGAG+AGG + Chr1:75647224-75647243 MsG0180004257.01.T01:CDS 40.0%
! CTCTCCATACCTTCTTTTCT+AGG - Chr1:75647226-75647245 None:intergenic 40.0%
! GAACGGTGTTAAAATGGAGT+TGG + Chr1:75646969-75646988 MsG0180004257.01.T01:CDS 40.0%
! GAGCATATGTCATTTGAGTC+AGG + Chr1:75649199-75649218 MsG0180004257.01.T01:three_prime_UTR 40.0%
! GGAGCATAACTTAGTTGGTA+AGG - Chr1:75649405-75649424 None:intergenic 40.0%
! GGTTTGAAGTTGGATTGTGT+TGG + Chr1:75647076-75647095 MsG0180004257.01.T01:intron 40.0%
AAGCACTTGTGAGACTGCTT+GGG + Chr1:75647506-75647525 MsG0180004257.01.T01:intron 45.0%
AGCAAGACATAACCAACCTC+AGG - Chr1:75648539-75648558 None:intergenic 45.0%
ATAAACTCCCATCGATACCC+AGG - Chr1:75648644-75648663 None:intergenic 45.0%
ATACATGAAAAAGGGGGAGG+AGG - Chr1:75647433-75647452 None:intergenic 45.0%
CACCAATGCCTTGATCTTGA+AGG + Chr1:75648038-75648057 MsG0180004257.01.T01:CDS 45.0%
CAGTGACAAGAACACGAACA+GGG - Chr1:75646174-75646193 None:intergenic 45.0%
GAGATACATGAAAAAGGGGG+AGG - Chr1:75647436-75647455 None:intergenic 45.0%
GATGATGCCTGGTATGTTGA+TGG + Chr1:75648279-75648298 MsG0180004257.01.T01:intron 45.0%
GCAATATCATCAGGGAACCT+GGG + Chr1:75648624-75648643 MsG0180004257.01.T01:intron 45.0%
GCTACAACTGATGTGGTTGA+GGG + Chr1:75647160-75647179 MsG0180004257.01.T01:CDS 45.0%
GCTGAAAAAGTGGTTCCATC+TGG + Chr1:75647817-75647836 MsG0180004257.01.T01:intron 45.0%
GCTGCATTCCCTCTTCTTAA+AGG + Chr1:75646997-75647016 MsG0180004257.01.T01:CDS 45.0%
GGAAACAAGCTGTCTAAGAG+AGG - Chr1:75649019-75649038 None:intergenic 45.0%
GGCGAATGGCAAATAGTTCA+AGG + Chr1:75648727-75648746 MsG0180004257.01.T01:CDS 45.0%
GTCTCACAAGTGCTTGCTTA+TGG - Chr1:75647501-75647520 None:intergenic 45.0%
GTTTCACGCCTTTCATGAGT+AGG + Chr1:75646006-75646025 MsG0180004257.01.T01:five_prime_UTR 45.0%
GTTTCTCAAGAGCAGAAGCT+TGG - Chr1:75647288-75647307 None:intergenic 45.0%
TCATAGCTACCGTCCAACAA+CGG + Chr1:75648420-75648439 MsG0180004257.01.T01:CDS 45.0%
TCTTGTCCCTATGATTGCCA+GGG + Chr1:75646876-75646895 MsG0180004257.01.T01:CDS 45.0%
TGCAATATCATCAGGGAACC+TGG + Chr1:75648623-75648642 MsG0180004257.01.T01:intron 45.0%
TGCTACAACTGATGTGGTTG+AGG + Chr1:75647159-75647178 MsG0180004257.01.T01:CDS 45.0%
! AGAAGAGCTTTCTGAGGAGA+AGG + Chr1:75648977-75648996 MsG0180004257.01.T01:CDS 45.0%
! CGGTGTTAAAATGGAGTTGG+TGG + Chr1:75646972-75646991 MsG0180004257.01.T01:CDS 45.0%
! GGTGGATTCCCTAGAAAAGA+AGG + Chr1:75647214-75647233 MsG0180004257.01.T01:CDS 45.0%
!! ATCAAGGCATTGGTGTTTGC+CGG - Chr1:75648033-75648052 None:intergenic 45.0%
!! CAGGGTACTGACTTGATGAA+TGG - Chr1:75648197-75648216 None:intergenic 45.0%
!! AATAATAATAATAATAATAG+AGG + Chr1:75645967-75645986 MsG0180004257.01.T01:five_prime_UTR 5.0%
ACAGTTGCGTGGTTGACATC+AGG - Chr1:75648216-75648235 None:intergenic 50.0%
ACATGCTGCTGCAAACTGCA+AGG + Chr1:75647306-75647325 MsG0180004257.01.T01:CDS 50.0%
ATAGCTACCGTCCAACAACG+GGG + Chr1:75648422-75648441 MsG0180004257.01.T01:CDS 50.0%
ATCACTCCCCTGGCAATCAT+AGG - Chr1:75646886-75646905 None:intergenic 50.0%
ATTGGACCATAACAGGGCTC+TGG + Chr1:75648107-75648126 MsG0180004257.01.T01:CDS 50.0%
CAAGCACTTGTGAGACTGCT+TGG + Chr1:75647505-75647524 MsG0180004257.01.T01:intron 50.0%
CAGTTGCGTGGTTGACATCA+GGG - Chr1:75648215-75648234 None:intergenic 50.0%
CATAGCTACCGTCCAACAAC+GGG + Chr1:75648421-75648440 MsG0180004257.01.T01:CDS 50.0%
CATCATCAGCCACGAGTTCA+CGG - Chr1:75648266-75648285 None:intergenic 50.0%
CCAGTGACAAGAACACGAAC+AGG - Chr1:75646175-75646194 None:intergenic 50.0%
CCTGTTCGTGTTCTTGTCAC+TGG + Chr1:75646172-75646191 MsG0180004257.01.T01:CDS 50.0%
CTCTTGTCCCTATGATTGCC+AGG + Chr1:75646875-75646894 MsG0180004257.01.T01:CDS 50.0%
CTTGTCCCTATGATTGCCAG+GGG + Chr1:75646877-75646896 MsG0180004257.01.T01:CDS 50.0%
GACAGCAGAAGAGCTTTCTG+AGG + Chr1:75648971-75648990 MsG0180004257.01.T01:CDS 50.0%
GAGCTCATGGAAACAGCTCA+TGG + Chr1:75647530-75647549 MsG0180004257.01.T01:intron 50.0%
GGATCTTACAATGTACCCGC+TGG + Chr1:75648670-75648689 MsG0180004257.01.T01:CDS 50.0%
GTCAAGCATGTGGAGGATCA+CGG - Chr1:75646926-75646945 None:intergenic 50.0%
TCAAGCATGTGGAGGATCAC+GGG - Chr1:75646925-75646944 None:intergenic 50.0%
TCACAACTCGCAATGGCGAA+TGG + Chr1:75648713-75648732 MsG0180004257.01.T01:CDS 50.0%
TCACTCCCCTGGCAATCATA+GGG - Chr1:75646885-75646904 None:intergenic 50.0%
TTCCCTGTCACAACTCGCAA+TGG + Chr1:75648706-75648725 MsG0180004257.01.T01:CDS 50.0%
TTGGACCATAACAGGGCTCT+GGG + Chr1:75648108-75648127 MsG0180004257.01.T01:CDS 50.0%
TTTGACCCAGAGCCCTGTTA+TGG - Chr1:75648116-75648135 None:intergenic 50.0%
! GCTTTCTGAGGAGAAGGCTT+TGG + Chr1:75648983-75649002 MsG0180004257.01.T01:CDS 50.0%
!! GTTCTAGGAACTCCTGAGGT+TGG + Chr1:75648524-75648543 MsG0180004257.01.T01:intron 50.0%
!! TCAAGGCATTGGTGTTTGCC+GGG - Chr1:75648032-75648051 None:intergenic 50.0%
!! TTGGGTTCTAGGAACTCCTG+AGG + Chr1:75648520-75648539 MsG0180004257.01.T01:CDS 50.0%
AAAGCCTGTCCGTGAACTCG+TGG + Chr1:75648254-75648273 MsG0180004257.01.T01:CDS 55.0%
AGACTGCTTGGGAGAGCTCA+TGG + Chr1:75647517-75647536 MsG0180004257.01.T01:intron 55.0%
ATTGCCAGGGGAGTGATGCT+CGG + Chr1:75646889-75646908 MsG0180004257.01.T01:CDS 55.0%
CCAGCAGCCGAATCACTGAA+CGG + Chr1:75646952-75646971 MsG0180004257.01.T01:CDS 55.0%
CGCCATTGCGAGTTGTGACA+GGG - Chr1:75648711-75648730 None:intergenic 55.0%
CTGGTGGGATGTCAAGCATG+TGG - Chr1:75646936-75646955 None:intergenic 55.0%
GATGTGGTTGAGGGATGCAC+TGG + Chr1:75647169-75647188 MsG0180004257.01.T01:CDS 55.0%
GCGTGAAACGACACCGCTTT+CGG - Chr1:75645995-75646014 None:intergenic 55.0%
GGCAACAACGAGAACCTGCA+TGG - Chr1:75648007-75648026 None:intergenic 55.0%
GGGGAGGAGGAACCATAGAA+TGG - Chr1:75647420-75647439 None:intergenic 55.0%
GGTTCTCGTTGTTGCCAACC+CGG + Chr1:75648011-75648030 MsG0180004257.01.T01:intron 55.0%
GTGGGATGTCAAGCATGTGG+AGG - Chr1:75646933-75646952 None:intergenic 55.0%
TCGCCATTGCGAGTTGTGAC+AGG - Chr1:75648712-75648731 None:intergenic 55.0%
TGATTGCAGCACCCCGTTGT+TGG - Chr1:75648436-75648455 None:intergenic 55.0%
! AACTCGTGGCTGATGATGCC+TGG + Chr1:75648268-75648287 MsG0180004257.01.T01:CDS 55.0%
! TCAGTGATTCGGCTGCTGGT+GGG - Chr1:75646951-75646970 None:intergenic 55.0%
! TTCAGTGATTCGGCTGCTGG+TGG - Chr1:75646952-75646971 None:intergenic 55.0%
!! CAGGGAACCTGGGTATCGAT+GGG + Chr1:75648634-75648653 MsG0180004257.01.T01:intron 55.0%
!! GTTCTTGTCACTGGTGCTGC+AGG + Chr1:75646181-75646200 MsG0180004257.01.T01:CDS 55.0%
!! TCAGGGAACCTGGGTATCGA+TGG + Chr1:75648633-75648652 MsG0180004257.01.T01:intron 55.0%
TCAGCCACGAGTTCACGGAC+AGG - Chr1:75648261-75648280 None:intergenic 60.0%
TGCAGCACCCCGTTGTTGGA+CGG - Chr1:75648432-75648451 None:intergenic 60.0%
! CCGTTCAGTGATTCGGCTGC+TGG - Chr1:75646955-75646974 None:intergenic 60.0%
!! GGCATTGGTGTTTGCCGGGT+TGG - Chr1:75648028-75648047 None:intergenic 60.0%
GCATGTGGAGGATCACGGGC+TGG - Chr1:75646921-75646940 None:intergenic 65.0%
GTGGTCACAAGCAGCGCTCG+CGG - Chr1:75648492-75648511 None:intergenic 65.0%
!! GGAGGATCACGGGCTGGTCA+GGG - Chr1:75646915-75646934 None:intergenic 65.0%
!! TGGAGGATCACGGGCTGGTC+AGG - Chr1:75646916-75646935 None:intergenic 65.0%
AGGGCCGAGCATCACTCCCC+TGG - Chr1:75646896-75646915 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 75645893 75649512 75645893 ID=MsG0180004257.01;Name=MsG0180004257.01
Chr1 mRNA 75645935 75649512 75645935 ID=MsG0180004257.01.T01;Parent=MsG0180004257.01;Name=MsG0180004257.01.T01;_AED=0.27;_eAED=0.27;_QI=191|1|1|1|0.85|0.75|8|487|332
Chr1 exon 75645935 75646088 75645935 ID=MsG0180004257.01.T01:exon:44644;Parent=MsG0180004257.01.T01
Chr1 exon 75646123 75646202 75646123 ID=MsG0180004257.01.T01:exon:44645;Parent=MsG0180004257.01.T01
Chr1 five_prime_UTR 75645935 75646088 75645935 ID=MsG0180004257.01.T01:five_prime_utr;Parent=MsG0180004257.01.T01
Chr1 five_prime_UTR 75646123 75646159 75646123 ID=MsG0180004257.01.T01:five_prime_utr;Parent=MsG0180004257.01.T01
Chr1 CDS 75646160 75646202 75646160 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 CDS 75646860 75647018 75646860 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 CDS 75647152 75647327 75647152 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 CDS 75648012 75648289 75648012 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 CDS 75648406 75648541 75648406 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 CDS 75648637 75648748 75648637 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 CDS 75648931 75649025 75648931 ID=MsG0180004257.01.T01:cds;Parent=MsG0180004257.01.T01
Chr1 three_prime_UTR 75649026 75649512 75649026 ID=MsG0180004257.01.T01:three_prime_utr;Parent=MsG0180004257.01.T01
Chr1 mRNA 75645893 75649512 75645893 ID=MsG0180004257.01.T02;Parent=MsG0180004257.01;Name=MsG0180004257.01.T02;_AED=0.26;_eAED=0.26;_QI=267|1|1|1|1|1|7|487|332
Chr1 exon 75645893 75646202 75645893 ID=MsG0180004257.01.T02:exon:44652;Parent=MsG0180004257.01.T02
Chr1 five_prime_UTR 75645893 75646159 75645893 ID=MsG0180004257.01.T02:five_prime_utr;Parent=MsG0180004257.01.T02
Chr1 CDS 75646160 75646202 75646160 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 CDS 75646860 75647018 75646860 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 CDS 75647152 75647327 75647152 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 CDS 75648012 75648289 75648012 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 CDS 75648406 75648541 75648406 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 CDS 75648637 75648748 75648637 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 CDS 75648931 75649025 75648931 ID=MsG0180004257.01.T02:cds;Parent=MsG0180004257.01.T02
Chr1 three_prime_UTR 75649026 75649512 75649026 ID=MsG0180004257.01.T02:three_prime_utr;Parent=MsG0180004257.01.T02
Gene Sequence

>MsG0180004257.01.T02

ATGGCTAAAGACCCTGTTCGTGTTCTTGTCACTGGTGCTGCAGGACAAATTGGATATGCTCTTGTCCCTATGATTGCCAGGGGAGTGATGCTCGGCCCTGACCAGCCCGTGATCCTCCACATGCTTGACATCCCACCAGCAGCCGAATCACTGAACGGTGTTAAAATGGAGTTGGTGGATGCTGCATTCCCTCTTCTTAAAGGTGTTGTTGCTACAACTGATGTGGTTGAGGGATGCACTGGAGTCAATATTGCTGTTATGGTTGGTGGATTCCCTAGAAAAGAAGGTATGGAGAGGAAAGATGTGATGACAAAAAATGTCTCTATTTACAAGTCCCAAGCTTCTGCTCTTGAGAAACATGCTGCTGCAAACTGCAAGGTTCTCGTTGTTGCCAACCCGGCAAACACCAATGCCTTGATCTTGAAGGAATTCGCTCCATCCATTCCTGAGAAAAACATTTCTTGTTTGACAAGATTGGACCATAACAGGGCTCTGGGTCAAATTTCTGAAAGACTGAACGTTGAAGTTTCTAATGTGAAAAATGTTATAATTTGGGGCAACCATTCATCAAGTCAGTACCCTGATGTCAACCACGCAACTGTTAAAATTGCTTCTGCAGAAAAGCCTGTCCGTGAACTCGTGGCTGATGATGCCTGGTTAAATGGAGAATTCATAGCTACCGTCCAACAACGGGGTGCTGCAATCATTAAAGCTAGAAAGCTTTCAAGTGCACTATCTGCCGCGAGCGCTGCTTGTGACCACATTAGAGATTGGGTTCTAGGAACTCCTGAGGGAACCTGGGTATCGATGGGAGTTTATTCTGATGGATCTTACAATGTACCCGCTGGACTAATCTATTCATTCCCTGTCACAACTCGCAATGGCGAATGGCAAATAGTTCAAGGACTATCAATTGATGAGTTTTCAAGGAAAAAATTGGACTTGACAGCAGAAGAGCTTTCTGAGGAGAAGGCTTTGGCTTACTCATGCCTCTCTTAG

>MsG0180004257.01.T01

ATGGCTAAAGACCCTGTTCGTGTTCTTGTCACTGGTGCTGCAGGACAAATTGGATATGCTCTTGTCCCTATGATTGCCAGGGGAGTGATGCTCGGCCCTGACCAGCCCGTGATCCTCCACATGCTTGACATCCCACCAGCAGCCGAATCACTGAACGGTGTTAAAATGGAGTTGGTGGATGCTGCATTCCCTCTTCTTAAAGGTGTTGTTGCTACAACTGATGTGGTTGAGGGATGCACTGGAGTCAATATTGCTGTTATGGTTGGTGGATTCCCTAGAAAAGAAGGTATGGAGAGGAAAGATGTGATGACAAAAAATGTCTCTATTTACAAGTCCCAAGCTTCTGCTCTTGAGAAACATGCTGCTGCAAACTGCAAGGTTCTCGTTGTTGCCAACCCGGCAAACACCAATGCCTTGATCTTGAAGGAATTCGCTCCATCCATTCCTGAGAAAAACATTTCTTGTTTGACAAGATTGGACCATAACAGGGCTCTGGGTCAAATTTCTGAAAGACTGAACGTTGAAGTTTCTAATGTGAAAAATGTTATAATTTGGGGCAACCATTCATCAAGTCAGTACCCTGATGTCAACCACGCAACTGTTAAAATTGCTTCTGCAGAAAAGCCTGTCCGTGAACTCGTGGCTGATGATGCCTGGTTAAATGGAGAATTCATAGCTACCGTCCAACAACGGGGTGCTGCAATCATTAAAGCTAGAAAGCTTTCAAGTGCACTATCTGCCGCGAGCGCTGCTTGTGACCACATTAGAGATTGGGTTCTAGGAACTCCTGAGGGAACCTGGGTATCGATGGGAGTTTATTCTGATGGATCTTACAATGTACCCGCTGGACTAATCTATTCATTCCCTGTCACAACTCGCAATGGCGAATGGCAAATAGTTCAAGGACTATCAATTGATGAGTTTTCAAGGAAAAAATTGGACTTGACAGCAGAAGAGCTTTCTGAGGAGAAGGCTTTGGCTTACTCATGCCTCTCTTAG
Protein sequence

>MsG0180004257.01.T02

MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVDAAFPLLKGVVATTDVVEGCTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVEVSNVKNVIIWGNHSSSQYPDVNHATVKIASAEKPVRELVADDAWLNGEFIATVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTTRNGEWQIVQGLSIDEFSRKKLDLTAEELSEEKALAYSCLS*

>MsG0180004257.01.T01

MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIPPAAESLNGVKMELVDAAFPLLKGVVATTDVVEGCTGVNIAVMVGGFPRKEGMERKDVMTKNVSIYKSQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVEVSNVKNVIIWGNHSSSQYPDVNHATVKIASAEKPVRELVADDAWLNGEFIATVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTTRNGEWQIVQGLSIDEFSRKKLDLTAEELSEEKALAYSCLS*