Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017551.01.T01 | GAU49970.1 | 87.166 | 187 | 21 | 1 | 1 | 184 | 1 | 187 | 9.43E-117 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017551.01.T01 | A0A2Z6PGB9 | 87.166 | 187 | 21 | 1 | 1 | 184 | 1 | 187 | 4.50e-117 | 339 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0380017551.01 | -0.831666 | 1.466742e-55 | 1.602511e-52 |
| MsG0080047869.01 | MsG0380017551.01 | -0.800012 | 1.759168e-48 | 8.103043e-46 |
| MsG0080047912.01 | MsG0380017551.01 | -0.806840 | 6.765698e-50 | 3.710408e-47 |
| MsG0080047944.01 | MsG0380017551.01 | -0.814936 | 1.195176e-51 | 8.127666e-49 |
| MsG0080047957.01 | MsG0380017551.01 | -0.851304 | 9.686749e-61 | 1.952305e-57 |
| MsG0080047959.01 | MsG0380017551.01 | -0.864433 | 1.220709e-64 | 3.832169e-61 |
| MsG0080048461.01 | MsG0380017551.01 | -0.827736 | 1.326830e-54 | 1.292004e-51 |
| MsG0080048462.01 | MsG0380017551.01 | -0.822268 | 2.594750e-53 | 2.161298e-50 |
| MsG0080048785.01 | MsG0380017551.01 | -0.819843 | 9.384699e-53 | 7.304098e-50 |
| MsG0080048809.01 | MsG0380017551.01 | 0.884143 | 2.467061e-71 | 1.609172e-67 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017551.01.T01 | MTR_3g108900 | 91.848 | 184 | 15 | 0 | 1 | 184 | 23 | 206 | 9.68e-117 | 330 |
Find 51 sgRNAs with CRISPR-Local
Find 277 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGCGAGGTACGAACGGGA+GGG | 0.566860 | 3:+97786201 | None:intergenic |
| AAGTATCGATCAGATCACGC+CGG | 0.740521 | 3:+97786010 | None:intergenic |
| ACGAAGATACAATTGACTTA+GGG | 0.534074 | 3:+97782904 | None:intergenic |
| ACGCTTCTGTTTCCGCGGTT+TGG | 0.395935 | 3:-97785954 | MsG0380017551.01.T01:CDS |
| ACGTAGACTGTGAACATTTG+CGG | 0.514078 | 3:+97786272 | None:intergenic |
| ACTAAACTTCGTTCTTGAGA+AGG | 0.577942 | 3:+97786182 | None:intergenic |
| ACTTCGTTCTTGAGAAGGCG+AGG | 0.622634 | 3:+97786187 | None:intergenic |
| AGGCGAGGTACGAACGGGAG+GGG | 0.553596 | 3:+97786202 | None:intergenic |
| AGTGGTGGAATCGGTGGAGG+CGG | 0.421097 | 3:+97786092 | None:intergenic |
| AGTTGATGTGGAGAGAGTGG+TGG | 0.659985 | 3:+97786077 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAGAAGCAAAAAAAGCA+GGG | + | Chr3:97784294-97784313 | None:intergenic | 30.0% | |
| AAACCAATGTCTTAAGGTTT+GGG | + | Chr3:97783716-97783735 | None:intergenic | 30.0% | |
| AATTTGGTCTACGAATTAAC+TGG | - | Chr3:97786013-97786032 | MsG0380017551.01.T01:CDS | 30.0% | |
| ACCAAAACTCTCTTAAGTTT+AGG | - | Chr3:97785001-97785020 | MsG0380017551.01.T01:intron | 30.0% | |
| AGAAAACATGAAATCCTAGA+AGG | + | Chr3:97785740-97785759 | None:intergenic | 30.0% | |
| ATCTAAATTCTCGAAACTGA+AGG | + | Chr3:97785625-97785644 | None:intergenic | 30.0% | |
| ATTAGGTACTAGTTGACATT+TGG | - | Chr3:97784493-97784512 | MsG0380017551.01.T01:intron | 30.0% | |
| CATAAGAAAAGCAAACTCAA+AGG | + | Chr3:97785578-97785597 | None:intergenic | 30.0% | |
| CCAAAACTCTCTTAAGTTTA+GGG | - | Chr3:97785002-97785021 | MsG0380017551.01.T01:intron | 30.0% | |
| CTAAATTCTAATTGACAGAG+GGG | + | Chr3:97784220-97784239 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 97782818 | 97786295 | 97782818 | ID=MsG0380017551.01;Name=MsG0380017551.01 |
| Chr3 | mRNA | 97782818 | 97786295 | 97782818 | ID=MsG0380017551.01.T01;Parent=MsG0380017551.01;Name=MsG0380017551.01.T01;_AED=0.46;_eAED=0.46;_QI=36|1|1|1|1|1|2|57|184 |
| Chr3 | exon | 97782818 | 97783057 | 97782818 | ID=MsG0380017551.01.T01:exon:21367;Parent=MsG0380017551.01.T01 |
| Chr3 | exon | 97785888 | 97786295 | 97785888 | ID=MsG0380017551.01.T01:exon:21366;Parent=MsG0380017551.01.T01 |
| Chr3 | five_prime_UTR | 97786260 | 97786295 | 97786260 | ID=MsG0380017551.01.T01:five_prime_utr;Parent=MsG0380017551.01.T01 |
| Chr3 | CDS | 97785888 | 97786259 | 97785888 | ID=MsG0380017551.01.T01:cds;Parent=MsG0380017551.01.T01 |
| Chr3 | CDS | 97782875 | 97783057 | 97782875 | ID=MsG0380017551.01.T01:cds;Parent=MsG0380017551.01.T01 |
| Chr3 | three_prime_UTR | 97782818 | 97782874 | 97782818 | ID=MsG0380017551.01.T01:three_prime_utr;Parent=MsG0380017551.01.T01 |
| Gene Sequence |
>MsG0380017551.01.T01 ATGGCTTACACCCTTCCTACGCTTCGTCTCTTCAACCCCTCCCGTTCGTACCTCGCCTTCTCAAGAACGAAGTTTAGTGTCACTTTACACGCGGCCTCTTTTTCAAACCGGACGAGTCAGCTCACCTTTAACTCAATGAGTCAACCCGCCTCCACCGATTCCACCACTCTCTCCACATCAACTGATTCGCCGGAAAACGTCGATGTTGTGGTCCAATACGTGGTGCTCCGGCGTGATCTGATCGATACTTGGCCATTAGGCAGTGTGGTTACTCAAGGTTGTCACGCTTCTGTTTCCGCGGTTTGGTCCAACAAAGACGATCCTGTCACCGTTGATTATTGTAGCCCCGACAAAATTGATTCTATGCACAAAGTCACACTTGAAGTGAAGGGAGAACCCCAGATCAAAAACTTGTCTGAGAAGCTGACATCTGGTGGGATCATTCATAAAGTGTGGGTTGAGCAACCTGAAAACATACCTACTTGCCTTGCTACAAAACCTTACCCTAAGTCAATTGTATCTTCGTATTTTAAGAAGTTGAAACTCTGCAAGTGA |
| Protein sequence |
>MsG0380017551.01.T01 MAYTLPTLRLFNPSRSYLAFSRTKFSVTLHAASFSNRTSQLTFNSMSQPASTDSTTLSTSTDSPENVDVVVQYVVLRRDLIDTWPLGSVVTQGCHASVSAVWSNKDDPVTVDYCSPDKIDSMHKVTLEVKGEPQIKNLSEKLTSGGIIHKVWVEQPENIPTCLATKPYPKSIVSSYFKKLKLCK* |
